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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Acta Aquatica: Aquatic Sciences Journal Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Dinamika Ekonomi - Jurnal Ekonomi dan Bisnis Biosaintifika: Journal of Biology & Biology Education Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Kelautan Nasional Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Penyuluhan Perikanan dan Kelautan J-ADIMAS (Jurnal Pengabdian kepada Masyarakat) Jurnal Abdi Insani Inspirasi Ekonomi : Jurnal Ekonomi Manajemen Jurnal ABM Mengabdi Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Rahmatan Lil’alamin Journal of Community Services (RLA) Jurnal Pepadu Jurnal Kedokteran Hewan Journal of Fisheries & Marine Journal of Vocational in Aquaculture (JAVA)

WIDANARNI WIDANARNI

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680

Author-ID : 415033

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Effectiveness of sinbiotic at different doses in Pacific white shrimp pond culture Sukenda, ,; Praseto, Rizki; Widanarni, ,
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The increasing demand of white shrimp Litopenaeus vannamei requires the application of intensive culture system. However, intensive culture system of white shrimp could increase the risk of disease outbreak. The application of sinbiotic may provide solution to the problem. This study was aimed to evaluate the effectiveness of the use of technical sinbiotic on the survival and growth of white shrimp in ponds. This study consisted of four treatments; treatment K (control), treatment A (probiotic 0.5% and prebiotic 1%), treatment B (probiotic 1% and prebiotic 2%), and treatment C (probiotic 2% and prebiotic 4%). The results showed that administration of sinbiotic had no significant differences on survival rate, growth rate, feed conversion ratio, size, and biomass of shrimp (P>0.05). However, based on analysis of business, sinbiotic A provided higher profits to the farmer (Rp10.230) compared to other symbiotic treatments and control. Keywords: Litopenaeus vannamei, sinbiotic, technical media ABSTRAK Permintaan terhadap udang vaname Litopenaeus vannamei yang semakin meningkat membuat sistem budidaya udang sebaiknya menggunakan sistem budidaya intensif. Namun demikian sistem intensif pada budidaya udang vaname dapat meningkatkan risiko timbulnya penyakit. Penggunaan sinbiotik diharapkan dapat memberikan solusi dalam mengatasi masalah tersebut. Penelitian ini bertujuan untuk menguji efektivitas penggunaan sinbiotik teknis terhadap sintasan, pertumbuhan, dan keuntungan usaha udang vaname yang dipelihara di tambak. Penelitian ini terdiri atas empat perlakuan, yaitu perlakuan K (kontrol), perlakuan A (probiotik 0,5% dan prebiotik 1%), perlakuan B (probiotik 1% dan prebiotik 2%), dan perlakuan C (probiotik 2% dan prebiotik 4%). Hasil penelitian menunjukkan bahwa pemberian sinbiotik tidak berbeda nyata dalam nilai sintasan, laju pertumbuhan, rasio konversi pakan, size, dan biomassa udang (P>0,05). Namun demikian, berdasarkan analisis usaha perlakuan A memberikan keuntungan lebih tinggi (Rp10.230) dibandingkan dengan perlakuan sinbiotik lainnya serta kontrol. Kata kunci: Litopenaeus vannamei, sinbiotik, media teknis

Stress responses of transportation on red tilapia which given feed containing chromium Rakhmawati, Rakhmawati,; Suprayudi, Muhammad Agus; Setiawati, Mia; Widanarni, Widanarni,; Junior, Muhammad Zairin; Jusadi, Dedi
Jurnal Akuakultur Indonesia Vol. 17 No. 1 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis study was conducted to evaluate stress responses of transportation on red tilapia Oreochromis sp. which given feed containing chromium. Three isonitrogenous and isocaloric experimental feeds were prepared, these diets were control (without chromium), CrPic 1 mg/kg, and CrYst 2 mg/kg supplementation in feed, all group were arranged triplicate. Satiation feeding was done three times a day. After a 60-day feeding experiment, the experimental fishes were fasted and distributed in polyethylene bags (N=60 fish/bag) containing 3 L of water, subjected to condition of transport simulation for 13 hours. Survival rate, levels of plasma cortisol, blood glucose, superoxide dismutase (SOD), and malondialdehyde (MDA) enzyme were observed at before transportation, after transportation, one day, and two days after transportation. The result showed that chromium supplementation reduced the levels of plasma cortisol before and after transportation, one day, and two days after transportation. Also, it decreased blood glucose compared with control significantly before transportation and one day after transportation. The SOD enzyme concentration increased significantly after fish was fed with feed containing chromium for 30 days, while the MDA enzyme concentration increased significantly after two days of transportation. However, there was no significant difference in the survival of red tilapia between treatments. The best result was obtained in the treatment of fish which fed with feed containing chromium. CrPic supplementation 1 mg/kg and CrYst 2 mg/kg increased the body resistance in red tilapia by decreasing the negative effect of stress while transportation. Keywords: stress, transportation, red tilapia, chromium ABSTRAKPenelitian dilakukan untuk mengevaluasi respons stres transportasi ikan nila merah Oreochromis sp. yang diberikan pakan yang mengandung kromium. Pada penelitian ini digunakan tiga jenis pakan, terdiri atas pakan tanpa suplementasi kromium (kontrol), pakan bersuplementasi kromium pikolinat (CrPic 1 mg/kg), dan kromium yeast (CrYst 2 mg/kg), semua perlakuan diulang sebanyak tiga ulangan. Pemberian pakan sebanyak tiga kali sehari dan dilakukan secara at satiation. Setelah 30 hari pemeliharaan, ikan uji dipuasakan dan didistribusikan dalam plastik polietilen (N=60 ekor ikan/kantong plastik) yang berisi 3 L air, dilakukan dengan simulasi transportasi selama 13 jam. Parameter yang diamati pada penelitian ini adalah kelangsungan hidup, kortisol, glukosa darah, enzim superoksida dismustase (SOD), dan malondialdehida (MDA) saat sebelum transportasi, sesaat setelah transportasi, sehari, dan dua hari setelah transportasi. Hasil yang didapatkan adalah suplementasi kromium menurunkan konsentrasi kortisol secara signifikan sebelum transportasi, sesaat, sehari, dan dua hari setelah transportasi. Suplementasi kromium menurunkan glukosa darah secara signifikan pada saat sebelum transportasi dan sehari setelah transportasi. Konsentrasi enzim SOD meningkat secara signifikan setelah pemberian pakan bersuplementasi kromium selama 30 hari, sedangkan konsentrasi enzim MDA meningkat secara signifikan setelah dua hari transportasi pada ikan yang diberi pakan bersuplementasi kromium. Namun, tidak ada perbedaan yang signifikan pada kelangsungan hidup ikan nila merah antarperlakuan. Hasil terbaik diperoleh pada perlakuan ikan dengan suplementasi kromium. Suplementasi 1 mg/kg CrPic dan 2 mg/kg CrYst dapat meningkatkan daya tahan tubuh pada budidaya ikan nila merah dengan menurunkan pengaruh negatif stres akibat transportasi. Kata kunci: stres, transportasi, nila merah, kromium

Grow-out of spiny lobster Panulirus sp. with high stocking density in controlled tanks Subhan, Rio Yusufi; Supriyono, Eddy; Widanarni, Widanarni,; Djokosetiyanto, Daniel
Jurnal Akuakultur Indonesia Vol. 17 No. 1 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThe aim of this research was to determine optimum stocking density for growing-out of spiny lobster Panulirus sp. in controlled tanks that conducted for 30 days. The experimental spiny lobsters have the initial average weight of 130.39 ± 0.32 g and initial average total length of 140.70 ± 0.06 mm. This study used completely randomized design with three different stocking densities (KT10: 10 ind/m3; KT18: 18 ind/m3; and KT26: 26 ind/m3) and two replications. The parameters observed in this study included water quality (temperature, pH, salinity, dissolved oxygen, and total ammonia nitrogen), physiological responses (total haemocyte count, haemolymph glucose, and frequency of molt), and production performances, such as growth, specific growth rate, feed conversion ratio, and survival rate. The results showed that the spiny lobster could be reared in high stocking density in controlled tanks. Water quality during the study in each treatment was; temperature 26.56–28.65oC, salinity 29.7–33.6 g/L, pH 7.5–8.5, dissolved oxygen 6.15–6.58 mg/L, and total ammonia nitrogen 0.11–0.34 mg/L. The best stocking densities for spiny lobster was 18 ind/m3 (KT18) with 2.5‒3.5×106cells/mL total haemocyte counts, 24.6‒28.3 mg/dL haemolymph glucose, and 38.37 ± 3.20% frequency of molt. The final average body weight and length were 145.06 ± 0.42 g and 142.77 ± 0.19 mm, respectively. The survival rate reached 86.11 ± 3.92% with a specific growth rate 0.35 ± 0.01%/day, and feed conversion ratio 7.87 ± 0.31.Keywords: high stocking density, Panulirus sp., physiological responses, productivity. ABSTRAKTujuan dari penelitian ini adalah untuk menentukan kepadatan terbaik dalam pembesaran lobster laut Panulirus sp. yang dipelihara dalam bak terkontrol selama 30 hari. Lobster laut yang digunakan pada awal penelitian memiliki bobot 130,39 ± 0,32 g dan panjang total 140,70 ± 0,06 mm. Penelitian dilakukan menggunakan rancangan acak lengkap dengan tiga perlakuan kepadatan berbeda, yaitu: 10 ekor/m3(KT10), 18 ekor/m3(KT18), dan 26 ekor/m3(KT26) dan dua ulangan. Parameter uji yang diamati dalam penelitian ini meliputi kualitas air (suhu, salinitas, pH, DO, dan TAN), respons fisiologis (total hemosit/THC, glukosa hemolim, dan frekuensi pergantian kulit), dan kinerja produksi meliputi pertumbuhan, laju pertumbuhan spesifik, rasio konversi pakan, dan tingkat kelangsungan hidup. Hasil penelitian menunjukkan bahwa lobster laut dapat dibesarkan dengan kepadatan tinggi dalam bak terkontrol. Pengukuran nilai kualitas air pada setiap perlakuan selama pemeliharaan adalah suhu berkisar 26,56–28,65oC, salinitas 29,7–33,6 g/L, pH 7,5–8,5, DO antara 6,15–6,58 mg/L dan TAN antara 0,11–034 mg/L. Perlakuan terbaik selama penelitian adalah dengan kepadatan 18 ekor/m3 (KT18) dengan nilai THC berkisar antara 2,5–3,5×106 sel/mL, glukosa hemolim 24,6–28,3 mg/dL,dan frekuensi pergantian kulit 38,37±3,20%. Bobot dan panjang lobster akhir rata-rata pada perlakuan tersebut masing-masing mencapai 145,06 ± 0,42 g, dan 142,77 ± 0,19 mm. Tingkat kelangsungan hidup mencapai 86,11 ± 3,92% dengan laju pertumbuhan spesifik 0,35 ± 0,01%/hari dan rasio konversi pakan selama penelitian adalah 7,87 ± 0,31.Kata kunci: padat pemeliharaan, Panulirus sp., produktivitas, respons fisiologis.

Selection of lactic acid bacteria as a probiotic and evaluated its performance on gnotobiotic catfish Clarias sp. Turnip, Enita Romasni; Widanarni, Widanarni,; Meryandini, Anja
Jurnal Akuakultur Indonesia Vol. 17 No. 1 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to select lactic acid bacteria (LAB) as a potential probiotic that producing anti‒microbial compounds in order to treat motile aeromonads septicemia diseases caused by Aeromonas hydrophila on catfish Clarias sp. and evaluated its performance on gnotobiotic catfish. The in vitro assay was done to select several LAB isolates based on antagonistic activity against pathogenic bacteria. The selected isolate was tested in vivo to observe their ability to improve growth performances of catfish. The study was conducted with five treatments consists of K‒ (normal catfish without addition probiotic, without challenge test), K+ (normal catfish without addition of probiotic, with challenge test), Np (normal catfish with addition of probiotic and challenge test), G (gnoto catfish without addition of probiotic, with challenge test), and Gp (gnoto catfish with addition of probiotic and challenge test). The results showed that the addition of Pediococcus pentosaceus E2211 as selected probiotic could increase survival rate, specific growth rate, and immune response towards infection of A. hydrophila. The best survival rate after challenge test was obtained in Np and Gp treatments (88.46%), followed by G treatment (65.38%), while the K+ was only 53.84%. The conclusion of this study was P. pentosaceus E2211 potentially used as a probiotic candidate for normal and gnotobiotic catfish. The presence of normal microflora with P. pentosaceus E2211 in Np treatment showed the best probiotic performance with daily growth rate 3.28%, feed conversion ratio 1.79, and total intestinal bacteria reached 108 CFU/mL significantly different from other treatments (P<0.05).Keywords: Aeromonas hydrophila, catfish, LAB, probiotic, screening ABSTRAKTujuan penelitian ini adalah menyeleksi bakteri asam laktat (BAL) sebagai probiotik potensial penghasil senyawa antimikrob guna menanggulangi penyakit motile aeromonad septicemia akibat Aeromonas hydrophila pada ikan lele Clarias sp. dan evaluasi kinerjanya pada ikan lele gnotobiotik. Pengujian in vitro dilakukan untuk menyeleksi beberapa isolat BAL sebagai kandidat probiotik berdasarkan aktivitas antagonis terhadap bakteri patogen. Isolat terpilih kemudian diuji in vivo untuk mengetahui kemampuannya dalam meningkatkan performa tumbuh ikan lele. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan, yaitu: K‒ (lele normal tanpa probiotik dan tanpa tanpa diuji tantang), K+ (lele normal tanpa probiotik dan diuji tantang), Np (lele normal diberi probiotik dan diuji tantang), G (lele gnoto tanpa probiotik dan diuji tantang), dan Gp (lele gnoto diberi probiotik dan diuji tantang). Hasil penelitian menunjukkan pemberian probiotik terpilih BAL Pediococcus pentosaceus E2211 mampu meningkatkan sintasan, laju pertumbuhan, dan respons imun ikan lele terhadap infeksi A. hydrophila. Sintasan terbaik pascauji tantang diperoleh pada perlakuan Np dan Gp yaitu sebesar 88,46%, diikuti perlakuan G sebesar 65,38%, sementara pada K+ hanya mencapai 53,84%. Kesimpulan dari penelitian ini ialah isolat BAL terpilih P. pentosaceus E2211 berpotensi sebagai kandidat probiotik untuk ikan lele normal dan lele gnotobiotik Clarias sp. Keberadaan mikroflora normal yang berasosiasi dengan P. pentosaceus E2211 pada perlakuan Np menunjukkan kinerja probiotik terbaik dengan nilai laju pertubuhan harian 3,28%, rasio konversi pakan 1,79 dan total bakteri usus mencapai 108 CFU/mL yang berbeda signifikan dibanding perlakuan lainnya (P<0,05).Kata kunci: A. hydrophila, BAL, ikan lele, probiotik, seleksi

Effectivity of prebiotic mannan oligosaccharides as the immunity enhancer and growth response on whiteleg shrimp Litopenaeus vannamei against white spot disease Prastiti, Linuwih Aluh; Yuhana, Munti; Widanarni, Widanarni,
Jurnal Akuakultur Indonesia Vol. 17 No. 1 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to evaluate the immune response and growth performance of white shrimp administered with prebiotic mannan oligosaccharides (MOS) with dosages of (0%, 0.2%, 0.4%, and 0.8% in diet) and used in the feeding trial. Shrimps (Litopenaeus vannamei) (the initial average weight was 3.416±0.064 g) were fed at satiation, three times a day. A completely randomized design was used in the study. Shrimps were cultured at the stock density of 15 shrimps 40/L for each treatment in triplicates. After 30 days of the feeding trial, shrimp were challenged with white spot syndrome virus filtrate by intramuscular injection. The total gut bacteria, total haemocyte count (THC), phenoloxydase (PO), and respiratory burst (RB) activity were observed 4 times, before the experiment, day 30th before challenge test, day 32nd after challenge test, and day 36th the end of the experiment. The shrimp survival was observed at day 36th to evaluate the immune responses. The results showed that THC, PO activity, RB activity, growth performance, and shrimp survival administered with prebiotic 0.8% were significantly higher (P<0.05) than control. The administration of prebiotic with dose 0.8% was the best result and could effectively improve the immune responses and growth performance of whiteleg shrimp. Keywords: prebiotic, whiteleg shrimp, white spot disease ABSTRAK Tujuan dari penelitian ini adalah untuk mengevaluasi respons imun dan performa pertumbuhan pada udang vaname yang diberi prebiotik mannan-oligosaccharides (MOS) dengan dosis berbeda (0%, 0,2%, 0,4%, dan 0,8%) pada pakan. Udang vaname (Litopenaeus vannamei) (dengan rata-rata bobot 3,41 ± 0,06 g) diberi pakan tiga kali sehari secara at satiation. Penelitian ini menggunakan rancangan acak lengkap. Udang dipelihara dengan kepadatan 15 ekor per 40/L pada setiap perlakuan dengan tiga kali pengulangan. Setelah 30 hari pemberian pakan, udang diuji tantang menggunakan white spot syndrome virus dengan diinjeksi secara intramuskular. Total bakteri usus, total haemocyte count (THC), aktivitas phenoloxydase (PO), dan aktivitas respiratory burst (RB) diamati 4 kali, yaitu sebelum perlakuan hari ke-30 sebelum uji tantang, hari ke-32 setelah uji tantang, dan hari ke-36 pada akhir penelitian. Hasil penelitian menunjukkan bahwa THC, aktivitas RB, aktivitas PO, performa pertumbuhan, dan kelangsungan hidup yang diberi prebiotik dengan dosis 0,8% lebih tinggi (P<0,05) jika dibandingkan dengan kontrol (dosis 0%). Pemberian prebiotik dengan dosis 0,8% merupakan hasil terbaik dan secara efektif mampu meningkatkan respons imun dan performa pertumbuhan pada udang vaname. Kata kunci: prebiotik, udang vaname, white spot disease

Microencapsulation of probiotics and its applications with prebiotic in Pacific white shrimp larvae through Artemia sp. Ramadhani, Dian Eka; Widanarni, Widanarni; Sukenda, Sukenda
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis study aimed to produce microencapsulated probiotic Pseudoalteromonas piscicida (1Ub) and evaluate it with prebiotic mannan-oligosaccharide (MOS) through the enrichment of Artemia sp., on bacterial population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Microencapsulation of probiotic was done by the freeze-drying method. The shrimp larvae were reared for 13 days and fed by the Artemia sp. enriched with microcapsule of probiotic 1Ub (10 g/L), prebiotic MOS (12 mg/L), synbiotic, and control without administration of microencapsulated probiotic and prebiotic, including negative (C-) and positive (C+) control. On the day 14, all of the experimental shrimp larvae except C- were challenged through immersion method with Vibrio harveyi MR5339 (107 CFU/mL). This study showed that the administration of microcapsule of probiotic 1Ub, prebiotic MOS, and synbiotic through the enrichment of Artemia sp. could increase the bacteria population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Moreover, synbiotic treatment demonstrated the best result compared to other treatments.Keywords: probiotic, prebiotic, synbiotic, Pacific white shrimp, microencapsulation ABSTRAKPenelitian ini bertujuan untuk membuat mikrokapsul probiotik Pseudoalteromonas piscicida (1Ub) dan mengevaluasinya dengan prebiotik mannan-oligosaccharides (MOS) melalui pengayaan Artemia sp. terhadap populasi bakteri, performa pertumbuhan, respons imun dan resistensi penyakit pada larva udang vaname. Mikroenkapsulasi probiotik dilakukan dengan metode freeze-drying. Larva udang dipelihara selama 13 hari dan diberi pakan Artemia sp. yang telah diperkaya dengan mikrokapsul probiotik 1Ub (10 g/L), prebiotik MOS (12 mg/L), sinbiotik, dan kontrol tanpa penambahan mikrokapsul probiotik dan prebiotik, termasuk kontrol negatif (C-) dan positif (C+). Pada hari ke-14, seluruh larva udang percobaan kecuali C- diuji tantang melalui metode perendaman dengan Vibrio harveyi MR5339 (107 CFU/mL). Hasil penelitian menunjukkan bahwa pemberian mikrokapsul probiotik 1Ub, prebiotik MOS, dan sinbiotik melalui pengayaan Artemia sp. dapat meningkatkan populasi bakteri, performa pertumbuhan, respons imun, dan resistensi penyakit pada larva udang vaname. Selain itu, perlakuan sinbiotik menunjukkan hasil terbaik dibandingkan perlakukan lainnya.Kata kunci : probiotik, prebiotik, sinbiotik, udang vaname, mikroenkapsulasi

Biofloc technology on the intensive aquaculture of bronze corydoras ornamental fish Corydoras aeneus with different stocking densities Diatin, Iis; Suprayudi, Muhammad Agus; Budiardi, Tatag; Harris, Enang; Widanarni, Widanarni
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Ornamental fish is non consumption fish which is an important source of Indonesian foreign exchange. The objective of this study is to analyze the productivity of bronze corydoras Corydoras aeneus ornamental fish through increased stocking density with biofloc technology. The average weight of the experimental corydoras was 0.61 ‒0.72 g with 2.32‒2.40 cm standard length. This study used a randomized design method with biofloc technology treatment in 3000, 4500, and 6000 fish/m2 stocking densities. The results showed that the daily length and weight-growth rate among treatments were not significantly different (P>0.05), while survival rate and the number of fish production on all treatments were significantly different (P<0.05). The water quality during the rearing period, such as temperature, pH, alkalinity, ammonia, nitrite, and nitrate, were in a tolerable range for corydoras culture. The total suspended solids tended to be higher due to higher stocking density. The best productivity using biofloc technology obtained from 6000 fish/m2 stocking density. Keywords: Biofloc technology, Corydoras aeneus, growth rate, stocking density, survival rate. ABSTRAK Ikan hias merupakan produk perikanan non konsumsi yang menjadi sumber devisa Indonesia yang cukup penting. Penelitian ini bertujuan untuk menganalisis produktivitas ikan hias koridoras melalui peningkatan padat tebar dengan teknologi bioflok. Ikan yang digunakan adalah ikan hias koridoras (Corydoras aeneus) berbobot 0,61‒0,72 g dan panjang baku 2,32‒2,40 cm. Penelitian ini menggunakan rancangan acak lengkap dengan perlakuan teknologi bioflok pada padat tebar 3000, 4500, dan 6000 ekor/m2. Hasil penelitian menunjukkan bahwa laju pertumbuhan harian panjang dan bobot antar perlakuan tidak berbeda nyata (P>0,05), sedangkan kelangsungan hidup dan jumlah produksi ikan pada semua perlakuan berbeda nyata (P<0,05). Nilai kualitas air selama pemeliharaan yakni suhu, pH, alkalinitas, amonia, nitrit, dan nitrat yang berada pada kisaran yang cukup baik untuk budidaya ikan. Total padatan tersuspensi cenderung tinggi akibat dari semakin tinggi padat tebar. Produktivitas terbaik pada budidaya ikan koridoras dengan teknologi bioflok adalah pada padat tebar 6000 ekor/m2. Kata kunci: Corydoras aeneus, kelangsungan hidup, padat tebar, pertumbuhan, teknologi bioflok

Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection Widanarni, Widanarni; Rahmi, Dewi; Gustilatov, Muhamad; Sukenda, Sukenda; Utami, Diah Ayu Satyari
Jurnal Akuakultur Indonesia Vol. 19 No. 2 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.19.2.118-130

ABSTRACT White spot disease caused by White Spot Syndrome Virus (WSSV) is the most serious viral disease and has a major impact on the decline in production of white shrimp farm. Improving the immune response through the application of prebiotic, probiotic and synbiotic is expected to be one of the environmentally friendly alternatives to prevent the disease. This study aimed to evaluate the effect of administrating Baccillus sp. NP5 probiotic, honey prebiotic and a combination both (synbiotic) in enhancing immune response and resistence of white shrimp to WSSV infection. This study consisted of five treatments and three replications, namely positive control (feeding without probiotics, prebiotics, and synbiotics then challenged with WSSV), negative control (feeding without probiotics, prebiotics, synbiotics, then injected with PBS), pro (feeding with the addition of Bacillus sp. NP5 probiotics then challenged against WSSV), pre (feeding with the addition of honey prebiotics then challenged against WSSV), and sin (feeding with the addition of synbiotics then challenged against WSSV). White shrimp with an average weight of 1.8±0.06 gram/shrimp were reared at a density of 15 shrimps per aquarium (60 cm x 30 cm x 30 cm and water height of 20 cm) for eight weeks, then challenge against WSSV at lethal dose (LD50) dose as much as 0.1 ml per shrimp. The results showed that the immune response and resistence of white shrimp after treatments of probiotics, prebiotics, and synbiotics were better than those in controls with the optimal found in prebiotics treatment. Keywords: Bacillus sp. NP5, honey, immune response, white shrimp, WSSV ABSTRAK White spot disease yang disebabkan oleh infeksi white spot syndrome virus (WSSV) merupakan penyakit viral paling serius dan berdampak besar terhadap penurunan produksi budidaya udang vaname. Perbaikan respons imun melalui aplikasi probiotik, prebiotik dan sinbiotik dapat menjadi salah satu alternatif ramah lingkungan untuk pencegahan serangan penyakit tersebut. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian probiotik Bacillus sp. NP5, prebiotik madu dan gabungan keduanya (sinbiotik) dalam meningkatkan respons imun dan resistansi udang vaname terhadap infeksi WSSV. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan yaitu kontrol positif (pemberian pakan tanpa probiotik, prebiotik, dan sinbiotik kemudian diuji tantang dengan WSSV), kontrol negatif (pemberian pakan tanpa probiotik, prebiotik, dan sinbiotik kemudian diinjeksi PBS), pro (pemberian pakan dengan penambahan probiotik Bacillus sp. NP5 kemudian diuji tantang dengan WSSV), pre (pemberian pakan dengan penambahan prebiotik madu kemudian diuji tantang WSSV), dan sin (pemberian pakan dengan penambahan sinbiotik kemudian diuji tantang dengan WSSV). Udang vaname dengan bobot rata-rata 1.8±0.06 gram/ekor dipelihara dengan kepadatan 15 ekor per akuarium (60 cm x 30 cm x 30 cm) selama delapan minggu, kemudian diuji tantang dengan WSSV sebanyak 0.1 ml per ekor pada dosis LD50. Hasil penelitian menunjukkan bahwa respons imun dan resistansi udang vaname setelah pemberian probiotik, prebiotik dan sinbiotik lebih baik (P<0.05) dibandingkan kontrol dengan hasil optimal pada perlakuan prebiotik. Kata kunci: Bacillus sp. NP5, madu, respons imun, udang vaname, WSSV.

Karakterisasi cairan fermentasi daun mangrove Avicennia marina dan daya hambatnya terhadap bakteri penyebab penyakit ice-ice Samsu Adi Rahman; Sukenda; Widanarni; Alimuddin; Julie Ekasari
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRAK Cairan fermentasi daun mangrove Avicennia marina mengandung mikroorganisme, nutrient, dan metabolit sekunder. Penelitian ini bertujuan untuk mengidentifikasi bakteri dan senyawa dalam cairan fermentasi daun mangrove A. marina dan mengukur daya hambatnya terhadap bakteri patogen Stenotrophomonas maltophilia penyebab penyakit ice-ice pada rumput laut. Hasil analisis molekuler dengan target gen 16S rRNA menunjukkan bahwa bakteri dalam cairan fermentasi terdiri atas delapan jenis Bacillus, yaitu Bacillus subtilis MSAR-01, Bacillus megaterium MSAR-02, Bacillus firmus MSAR-03, Bacillus thuringiensis MSAR-04, Bacillus subterranerus MSAR-05, Bacillus vietnamensis MSAR-06, Bacillus sp. MSAR-07, Bacillus circulans MSAR-08, dengan daya hambat terbaik ditunjukkan oleh B. subtilis MSAR-01, B. vietnamensis MSAR-06, dan Bacillus sp. MSAR-07. Pemberian asam laktat, bakteriosin, cairan fermentasi total, dan supernatan sebanyak 15 mL menghasilkan daya hambat terhadap bakteri S. maltophilia lebih baik daripada menggunakan salah satu atau kombinasi beberapa jenis bakteri isolat. Daya hambat cairan fermentasi dan supernatan yang diperkaya bakteri tunggal lebih baik daripada pengayaan kombinasi bakteri. Kata kunci: Avicennia marina, fermentasi, ice-ice, mangrove

Growth performance and immune response of catfish Clarias sp. given probiotics Bacillus megaterium PTB 1.4 and Pediococcus pentosaceus E2211 Muhammad Subhan Hamka; Anja Meryandini; Widanarni
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Motile aeromonad septicaemia (MAS) in catfish can be done by improving the immune responses through probiotic administration. Co-administering probiotics producing digestive enzymes are expected to have an impact on fish growth. This study was aimed to evaluate the effectivity of probiotics B. megaterium PTB 1.4 and P. pentosaceus E2211 to improve the growth performance and immune response of catfish infected by Aeromonas hydrophila. Catfish with the initial body weight of 7.36 ± 0.21 g were reared in a pond. This study was conducted with five treatments, i. e. K- (without probiotic addition and A. hydrophila injection), K+ (no probiotic addition with A. hydrophila injection), Bm (B. megaterium PTB 1.4 addition and A. hydrophila injection), Pp (P. pentosaceus E2211 addition and A. hydrophila injection), and Bm+Pp (B. megaterium PTB 1.4 addition and P. pentosaceus E2211 and A. hydrophila injection). The study results showed that Bm, Pp, and Bm+Pp treatments were able to improve the growth performance of catfish including digestive enzyme activity, daily growth rate, feed conversion ratio, and final biomass with the best results was Bm+Pp treatment. The immune response of catfish before and after the challenge test showed better survival rate, higher total leukocytes, phagocytic activity, respiratory burst, and lysozyme activity on Bm, Pp, and Bm+Pp treatment (P<0.05) than the control treatment. In conclusion, the combination of probiotics B. megaterium PTB 1.4 and P. pentosaceus E2211 in feed synergistically improved the growth performance and immune response on catfish against A. hydrophila infection. Keywords: Aeromonas hydrophila, catfish, growth performance, immune response, probiotics ABSTRAK Motile aeromonad septicaemia (MAS) pada ikan lele dapat dikendalikan melalui respons imun inang dengan pemberian probiotik. Pemberian bersama probiotik penghasil enzim pencernaan diharapkan dapat berdampak pada pertumbuhan ikan. Penelitian ini bertujuan mengevaluasi efektivitas pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 terhadap kinerja pertumbuhan dan respons imun ikan lele terhadap infeksi A. hydrophila. Ikan lele dengan bobot 7.36 ± 0.21 g dipelihara pada kolam beton yang diberi waring. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan, yaitu: K- (tanpa probiotik dan tanpa diinjeksi A. hydrophila), K+ (tanpa probiotik dan diinjeksi A. hydrophila), Bm (diberi B. megaterium PTB 1.4 dan diinjeksi A. hydrophila), Pp (diberi P. pentosaceus E2211 dan diinjeksi A. hydrophila), dan Bm+Pp (diberi B. megaterium PTB 1.4 dan P. pentosaceus E2211 dan diinjeksi A. hydrophila). Hasil penelitian menunjukkan perlakuan Bm, Pp, dan Bm+Pp mampu meningkatkan pertumbuhan ikan lele. Aktivitas enzim pencernaan, laju pertumbuhan harian, rasio konversi pakan, dan biomassa panen dengan hasil terbaik yaitu perlakuan Bm+Pp. Respons imun ikan lele dengan kombinasi probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 mampu meningkatkan sintasan, total leukosit, aktivitas fagositosis, respiratory burst, dan aktivitas lisozim, baik pada sebelum dan setelah uji tantang. Pemberian kombinasi probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 pada pakan mampu bekerja sinergis dalam meningkatkan kinerja pertumbuhan dan respons imun ikan lele terhadap infeksi A. hydrophila. Kata kunci: A. hydrophila, ikan lele, kinerja pertumbuhan, probiotik, respons imun

Co-Authors . Sunarto A. Sunarma A. Suwanto A.J. Sihombing Achmad Farouq Ade Dwi Sasanti ADNI OKTAVIANA Afiff , Usamah Agus Oman Sudrajat Aldy Mulyadin Alfabetian Harjuno Condro Haditomo Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimudin Alimudin, Alimudin Alit Brilliant Angela Mariana Kusumastuti, Angela Mariana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Al Rosjidi Anis Zubaidah Anja Meryandini Anja Meryandini Anjarweni, Vika Atinia Annisa Astri Anggraeni ANTONIUS SUWANTO Antonius Suwanto Ardana Kurniaji Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Asri Sutanti, Asri Asrido, Farhan Astari, Belinda Ayi Santika Bagus Ansani Takwin Bako, Surandha Benny Heltonika Bunyamin Bunyamin Cahyawati, Nadia Catur A. Pebrianto D. Augustine D. Djokosetiyanto D. Meha D. Shafruddin D.D. Mailana D.T. Soelistyowati Daniel Djokosetiyanto DEBY YUNIASARI DEDI JUSADI Dendi Hidayatullah, Dendi Dewi Nurhayati Dewi Rahmi DIAH AYU SATYARI UTAMI DIANA ELIZABETH WATURANGI Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Agung Saputra Dwi Nita Aryani E. Ayuzar Eddy Supriyono Edi Sudiarto Eko Sudjawoto Elizabeth Waturangi, Diana Enang Harris Enang Harris Enzeline, Valensia Evi Maria Fadilah, Iin Nur Fahmi Rajab Fauzi, Arini Resti Ferymon Mahulette Ferymon Mahulette, Ferymon Fiska Puspita Fitria Novianti Frid Agustinus Gentiga Muhammad Zairin Ghita Ryan Septiani Gustilatov, Muhamad H.J. Bugri Hamida Pattah Hamsah Hamsah Hamtini - Hamtini Hany Handajani Hariadi, Sugeng Harton Arfah Hasan Nasrullah Huda Salahudin Darusman Huda Shalahudin Darusman I. Effendi I. Tepu Iis Diatin Iis Sumartini Iman Rusmana Inem Ode Iqbal Kurniawinata, Mohamad Irma Melati Irzal Effendi Istiqomah, Amalia Ita Apriani Jeanni Indah Noermala Julie Ekasari Julyantoro, Pande Gde Sasmita Kautsar, Badar Kukuh Nirmala Lastriliah, Mira Lilik Setiyaningsih Linuwih Aluh Prastiti M. Tri Djoko Sunarno M. Yusuf Arifin M. Zairin Junior M.A. Lidaenni M.S. Arifin Marli, Marli Mas Tri Djoko Sunarno, Mas Tri Djoko Maulana, Fajar Mia Setiawati Mohammad Faizal Ulkhaq MUHAMMAD AGUS SUPRAYUDI Muhammad Junior Zairin Muhammad Subhan Hamka Muhammad Zairin Jr. Mulyasari Mulyasari Mulyasari Mulyasari MUNTI YUHANA Nasrullah, Hasan NISA RACHMANIA MUBARIK Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah O. Carman Ode, Inem Prassetyo Dwi Dhany Wijaya Puguh Widagdo Putra, The Best Akbar Esa Rahmi, Kurnia Anggraini Rakhmawati, Rakhmawati, Ramadhani, Dian Eka Ratnadilla, Thamara Reza Samsudin Rifqah Pratiwi Rina Rahmawati, Rina Rio Yusufi Subhan Rizki Praseto, Rizki Rizkiyanti, Ita Rr. Bellya Anasti Maharani Ruku Ratu Borut S. Sarah Safira Qisthina Ayuningtyas, Safira Qisthina Salamah Salamah Samsu Adi Rahman Saputra, Damar Auliawan Saputri, Rika Ani Septiani, Ghita Ryan Siregar, Khoirotun Nisa SITI MARYAM Siti Munfaqiroh Sri Nuryati Sri Nuryati Suci antoro Sudrajat, R Herman Sugiyo Hadi Pranoto Sujaka Nugraha Sujono Sujono Sukenda Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Suprianto, Dedi Suryadi Saputra Tambun, Andreas Tatag Budiardi Tatik Mufidah Tatik Mufidah Taufiq Abdullah Titin Kurniasih Tri Heru Prihadi Tri Rezeki, Nanda Tsani Untsa, Agista Turnip, Enita Romasni Vinasyam, Apriana W. Efiyanti Wahyu Afrilasari WAODE MUNAENI Wasmen Manalu Wicaksono, Baref Agung Wida Lesmanawati Wijaya, Sella Septian Windu Sukenda Wira H Saputra Wiyarni Wiyarni Y. Hadiroseyani Yani Aryati Yanti Inneke Nababan Yeni Elisdiana Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yuke Eliyani Yuke Eliyani Yulianti, Susy Anisa Yunarty Yunarty Zafril Imran Azwar Zairin Jr., Muhammad

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