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All Journal Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner Indonesian Journal of Applied Sciences Media Kedokteran Hewan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Biosains Pascasarjana JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Aceh Journal of Animal Science Jurnal Ilmiah Kedokteran Wijaya Kusuma Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Ilmu Nutrisi dan Teknologi Pakan Jurnal Medik Veteriner NERSMID: Jurnal Keperwatan dan Kebidanan Jurnal Ilmiah Fillia Cendekia Journal of Aquaculture and Fish Health Veterinary Biomedical and Clinical Journal Jurnal Ilmu Peternakan Terapan Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology Bioscientist : Jurnal Ilmiah Biologi journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Fisheries & Marine
Mufasirin
Divisi Parasitologi Veteriner, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

 1 
Deteksi Cryptosporidium canis pada Anjing di Kota Surabaya (CRYPTOSPORIDIUM CANIS DETECTION IN DOGS IN THE CITY OF SURABAYA) Romy Muhammad Dary Mufa; Nunuk Dyah Retno Lastuti; Fedik Abdul Rantam; Lucia Tri Suwanti; Endang Suprihati; Didik Handijatno; Mufasirin Mufasirin
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cryptosporidiosis is a disease caused by Cryptosporidium spp. protozoan parasites and are zoonotic. Cryptosporidium canis is the main species that infects dogs. Transmission of C. canis in dogs to humans is possible. This study aims to detect microscopic C. canis infection based on morphology and molecularity using the Polymerase Chain Reaction (PCR) in dogs in Surabaya City. A total of 80 diarrhea dog feces samples were taken from Animal Hospitals and animal clinics in several areas in the Surabaya City, then added potassium dichromate and stored at 4OC. Detection was made of the presence of Cryptosporidium spp. oocysts microscopically which is then confirmed by molecular examination using the PCR method. The results showed 40 positive samples containing Cryptosporidium spp., oocysts, with a size of 2-6 ?m. Ten samples from the total positive sample of Cryptosporidium spp. oocysts by microscopic examination, with the PCR test there were seven positive samples of C. canis. Based on the results of the study it can be concluded that the species that causes Cryptosporidiosis in dogs in Surabaya City is C. canis. The high cases of Cryptosporidiosis in dogs can be a warning to be able to prevent Cryptosporidium spp. infections, especially in pets that have the potential as a reservoir in spreading disease.
Deteksi Molekuler Blastocystis sp. pada Babi Terinfeksi di Kabupaten Tabanan dan Badung, Provinsi Bali, Indonesia (MOLECULAR DETECTION OF BLASTOCYSTIS INFECTION IN PIGS AT TABANAN AND BADUNG DISTRICT, BALI PROVINCE, INDONESIA) Doohan Mahendra; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Endang Suprihati; Wiwik Misaco Yuniarti; Ni Komang Aprilina Widisuputri
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aims to identify morphology and molecular of Blastocystis infection in pigs in the Tabanan and Badung District, Bali Province, Indonesia. 100 samples of pig stool were taken, fifty samples from the Tabanan and fifty samples from Badung District. Blastocystis sp. isolated and identified microscopically and confirmed by polymerase chain reaction (PCR) as a molecular identification method. 63% (63/100) Blastocystis is found in pig feces from these two District by microscopic detection. Positive samples were found 27 samples from Badung and 36 samples from Tabanan. Five samples were taken from each District conducted a confirmation test using the PCR method and found 60% (6/10) positive samples, two samples from Badung and four samples from Tabanan. The results of this study indicate that in healthy pigs, they also contain parasites that have the potential to spread disease to humans. Therefore, the high incidence of Blastocystis in pigs can be an alarm or warning to prevent Blastocystis infection from livestock that can potentially to be a reservoir in the spread of disease.
Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya Fransiska Cicilia Beka; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Koesdarto Koesdarto; Mufasirin Mufasirin; Endang Suprihati; Dyah Ayu Kurniawati
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.657 KB) | DOI: 10.19087/jveteriner.2021.22.3.383

Abstract

Penelitian ini bertujuan untuk mendeteksi E. suis pada babi yang dipotong di Rumah PotongHewan (RPH) Pegirian, Surabaya. Sebanyak 63 sampel feses babi dikoleksi dari RPH Pegirian Surabaya. Deteksi morfologis Entamoeba spp. dilakukan dengan metode apung (analisis parasitologi). Selain itu, penentuan spesies E. suis dilakukan dengan PCR menggunakan primer Esuis764 (5’-ATC AAA TCA ATT AGG CAT AAC TA-3’) dan Esuis765 (5’-AAT TAA AAC CTT ACG GCT TTA AA-3’) dan prodak PCR yang diharapkan adalah 320 bp. DNA dari sampel positif diekstraksi dengan DNAzol. PCR dilakukan pada beberapa sampel positif yang dipilih secara acak dari analisis parasitologi. Hasil penelitian menunjukkan 57,14% (36/63) sampel secara morfologis positif Entamoeba sp. Hasil penentuan spesies berdasarkan analisis molekuler menemukan bahwa protozoa yang menginveksi babi di Rumah Potong Hewan Pegirian, Surabaya adalah E. suis.
Perbedaan Larva Stadium Kedua dan L2 Toxocara canis pada Jaringan Mencit Menggunakan Scanning Electron Microscopy (THE DIFFERENCES BETWEEN SECOND STAGE LARVAE AND L2 TOXOCARA CANIS ON MICE TISSUE BY USING SCANNING ELECTRON MICROSCOPY) Vindo Rossy Pertiwi; Kusnoto Kusnoto; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Mufasirin Mufasirin
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.144 KB) | DOI: 10.19087/jveteriner.2019.20.3.390

Abstract

Toxocariasis is one of zoonosis diseases that caused by Toxocara spp. that is Toxocara canis. Toxocara canis has several stages until it can infect animals and humans, namely the egg stage, larvae first stage (L1), larvae second stage (L2), larvae third stage (L3) to adult worms. Studies about the L2 and L2 tissue of T. canis found in paratenic hosts using Scanning Electron Microscopy (SEM) have not been widely performed. Some of the causes include L2 being not easily to found and identified, so research rarely raises the ultrastructural morphology of L2 and L2 tissues. Knowledge about the ultrastructural morphology of L2 and L2 tissue of T. canis worms is very important to determining the diagnosis, especially the etiological diagnosis. The purpose of this study is to detected morphology of L2 and L2 tissues of T. canis using SEM. Samples from this study is faeces of dogs that infected with toxocariasis and the digestive tract of dogs obtained from dog slaughter houses. The sample is an adult worm of T. canis; the female worm is dissected and taken uterus to collect worm eggs. The results of this study on microscopic and optilab examination showed a difference between L2 and L2 tissue that the length of L2 hatched from embryonic eggs was 390 ìm and with a width of 23.4 ìm at the midpoint of the body. Larvae second stage length from the infected somatic tissue is 410 ìm and the width is 22.5 ìm at the midpoint, and then difference in dorsal lip, cuticles, body ring, cervical alae, buccal capsul, tail.
Vaksininasi Protein Ekskretori-Sekretori Toxoplasma gondii Hasil Biakan in vivo Membangkitkan Respons Imun Non Protektif (THE VACCINATION OF Toxoplasma gondii EXCRETORY-EXCRETORY PROTEINS FROM IN VIVO CULTURE ENHANCED IMMUNE RESPONSE UNABLE PROTECTIVE) Mufasirin .; Endang Suprihati
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research was to explore the immune response and protectiveness of mice whichwere vaccinated with Toxoplasma gondii excretory-secretory proteins produced from in vivo culture. A totalof 50  Balb/c strain mice were allotted  into five groups. Mice in group 1 to 3 were vaccinated with 20.7 kDa,35.3 kDa, and 100.9 kDa of the protein, respectively.  Whereas mice in group 4 were given total  excretory-secretory antigen ( total ESA5), and mice in group 5 were used as  control (PBS).  Booster  vaccinated wasconducted at two weeks following the first vaccination. Prior the challenge test, five mice were sacrificed forimmunologlobulin-G (IgG) analysis. The analysis of IgG using enzyme linked immunosorbent assay (ELISA).Challenge test used 1x103 T. gondii tachyzoites which were given to the animals   intraperitoneally.  Theresults showed that vaccination using  T.gondii excretory-secretory proteins as well as T. gondii total ESAscould enhanced immune response as detected by the markedly  presence of T. gondii  IgG.  However, thiswas unable to protect against T. gondii RH strain infection.
Co-Authors Abdul Samik Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Adikara, Tatang Santanu Al arif, Mohammad Anam Al Arif, Muhammad Anam Alasrorik, Muhammad Hizbulloh Alfina Azkiana Amalia Rosydinasari Rosydinasari Anam Al Arif andi jayawardhana Andi Jayawardhana Ardianto Ardianto Arif Pratiwi Arif Rahman Nurdianto Arimbi Aryaloka, Suhita Aryati Aryati Aryati Aryati Azizah Bilqis Nurkarimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Benjamin Christoffel Tehupuring, Benjamin Christoffel Berlina, Cyrcilia Relita Boedi Setiawan Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Desty Shafira Dewi Purwatiningsih Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Djoko Legowo Djoko Legowo Doohan Mahendra Doohan Mahendra DWI PUTRI RAHMAWATI Dyah Ayu Kurniawati Dyah Ayu Kurniawati Eduardus Bimo Aksono Herupradoto Edward Yonas Kristijanto Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Elok Apriliawati Emy Koestanti Sabdoningrum Endah Rochmatika Endang Suprihati Endang Suprihati Erma Safitri Fakhryyah Maharani Deviyanti Farezi, Reza Adrio Fatmawati, Mira Fatmawati Fauziah Fitri Hernanto Fedik Abdul Rantam Firdaus , Muhammad Aviv Fitri, Paraswita Eindah Fransiska Cicilia Beka Frida Aulya Arningdiah Galaxy Guardian Gunanti Mahasri Haditanojo, Wiyanto Hana Eliyani Hana Eliyani Hani Plumeriastuti Hanna Harnida, Hanna Hartono Hartono Hayuning Nurrodhiya Heni Puspitasari Herdiansyah, Akbar Dimas Hermin Ratnani Herry Agoes Hermadi Hidanah , Sri Hidanah, Sri Ihda Hanny, Khurun'In Fadia IMAM MUSTOFA Indasari, Elly Nur Ira Sari Yudaniayanti Istiana, Izzatul Iwan Sahrial Hamid Jayanti Dian Eka Sari, Jayanti Dian Joko Prastowo Jumria, Andi Kenconojati, Hapsari Khairullah, Aswin Rafif Khalissa Farah Alifia Koesdarto Koesdarto Kurniawan, Muhammad 'Ahdi Kurniawan, Muhammad ‘Ahdi Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lestari Wilujeng Lilik Maslachah Lisnanti, Ertika Fitri Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Lutfiah Annisa Billa Luthfiyyah Nur Afifah Siswandi Mafruchati, Maslichah Maghfiroh, Nurutin Tutur Bifatikhatil M Masdiana C Padaga Melani Anggraini Melanie Aulia Ashfiyah Mirni Lamid Mirni Lamid Mochammad Amin Alamsjah Mohamad Yusuf Mohammad Guritno Suryokusumo Moses, Ikechukwu Benjamin Mufa, Ramy Inas Mahirah Muhamad Amin Muhammad Ahdi Kurniawan Muhammad Al-Syafiq bin Abdul Halim Muhammad Ridwan Muslimah, Bintang Mustofa Helmi Effendi Nedi Nedi Nenny Harijani Ni Komang Aprilina Widisuputri Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nurdianti Nurdianti Nurdianto, Arif Rahman Nurfaizah, Diza Ulya Nusdianto Triakoso Nusdianto Triakoso Nuurin Ajrin Karim Ochtavia, Sherly P Kumaladewi Permata Sari, Dian Ayu Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastoetik Poedji Hastutiek Poetranto, Emmanuel Djoko Pradana, Giffari Danindra Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Pratiwi, Arif Primarizky, Hardany Puput Ade Wahyuningtyas Puput, Sesa Purbowati, Tri Endah Puspikawati, Septa Indra Rahadju Ernawati Rahardjo, Adi Prijo Rahmandari, Dina Agylia Rahmatullah, Aldin Akbar Ramadhana Ramadhana Ramadhanty, Miladhiyah Nabila Ratna Damayanti Rekasni Adallin A/P Morgan A/P Morgan Retno Yuli Rimayanti Rina Vitriasari Riwu, Katty Hendriana Priscilia Romy Muhammad Dary Mufa Romziah Sidik Rosyta, Pegy Sabdoningrum, Emy Koestanti Saksono, Bayu Sapto Andriyono Sari, Fifin Kurnia Septian Hakim Susantoputro Setiawan Koesdarto Setiawan Kusdarto Sherly Ochtavia Silfia, Himatul Ilma Soeharsono Soeharsono Soetojo Soetojo Sri Agus Sudjarwo Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Sukarno, Gerda Sumartono Sumartono Sunarso, Agus Susilo, Rahadian Indarto Talita Yuanda Reksa Taufan Ary Handoko Tita Damayanti Lestari Tony Hartono Tri Suwanti, Lucia Tri Wahyu Suprayogi Trifena Pristi Anindyta Tyasningsih, Wiwiek Vindo Rossy Pertiwi Wahidan Qodiip Maulana Warda Nafalizza Efendi Warsito , Sunaryo Hadi Warsito, Sunaryo Hadi Warsito, Sunaryo Hadi Widi Nugroho Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Win Darmanto Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wurlina, W Yudanianyi, Ira Sari Yudaniayanti , Irasari Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad ZULFAH