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Analisis Kandungan Merkuri Pada Krim Pemutih Yang Beredar Di Kota Manado Parengkuan, Kissi; Fatimawali, Fatimawali; Citraningtyas, Gayatri
PHARMACON Vol 2, No 1 (2013): pharmacon
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.886

Abstract

ABSTRACT Whitening cream is a mix of chemicals which whiten skin or bleach black fleck on the skin. The use of Mercury in whitening cream effects many things, such as skin color changes, and at the end cause black flecks, allergy, and irritation. The use of high dose cause permanent brain damage and cancer. The objectives of this research were to identify and determine Mercury (Hg) in some whitening creams marketed in Manado City.Sample of whitening cream were 10 samples.Identification of Mercury using color test, and Cold Vapour-Atomic Absorption Spectrophotometry (CVAAS). The results shows that five samples contains Mercury (Hg)0,0004 ppm, 0,0003 ppm, 0,0006 ppm, 0,0004 ppm, and 0,0005 ppmwithin ten samples analysis. Key words : Whitening cream, Mercury, CV-AAS (Cold Vapour Atomic AbsorbtionSpectrophotometry).
ANALISIS BORAKS PADA TAHU YANG DIPRODUKSI DI KOTA MANADO Triastuti, Endang; Fatimawali, Fatimawali; Runtuwene, Max R.J.
PHARMACON Vol 2, No 1 (2013): pharmacon
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.910

Abstract

ABSTRACK Tofu is a food made by coagulating soy juice and then pressing the resulting curds into soft white blocks. The use of food additive in food process needs to be aware. Borax were restricted to be used, it cause brain damage, liver, and kidney. Prolonged use may cause death. The objectives of this research was to find out the presence of borax in tofu which produced in Manado city. Investigated tofu were sample A (Bahu), B (Batukota I), C (Batukota II), D (Kleak), and E (Pakowa). Borax were evaluated by flame test, curcuma paper color test, turmeric color test and spectrophotometry UV-vis. The results shows that there were five samples that did not contains borax using flame test, curcuma paper test, turmeric paper test and Spectrophotometry UV-Vis analysis.   Key words : Borax, Tofu, Qualitative analysis, Spectrophotometry UV-Vis, Manado.
UJI EFEK ANTIINFLAMASI EKSTRAK ETANOL KULIT BATANG JAMBU BIJI (PSIDIUM GUAJAVA) TERHADAP EDEMA KAKI TIKUS JANTAN GALUR WISTAR Lanti, Deliyana; Fatimawali, Fatimawali; Wullur, Adeanne
PHARMACON Vol 1, No 2 (2012)
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.1.2012.508

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Inflamation is a protective response of body from injury of cell. Edema is one ofinflammation sign. Guava stem leather contains of flavonoid which has potency to inhibitedema. The aims of this research were to study antiinflamation effect of ethanolic extractofguava stem leather (Psidium guajava L.) on edema of white mice strain wistar legs.Thesubjects in this research were 25 wistar male which divided into 5 groups, namely negativecontrol (aquades), positive control (indometasin) and extract group with concentration 1%,2% and 4%. Every group consists of five mices. Aquades, indometasin and extractsupplementation were done per oral, followed with 5% of white egg induction on mice leftleglead to edema formation.Measurement of edema volume was done using plestimometer,and observation of edema were made every hour during 7 hours. Data which obtained wereanalyzed with ANOVA, if there is a differences between treatment, analysis continue to LSDtest. The result shows that supplementation of ethanolic extract of guava stem leatherconcentration 1% and 2 % decrease edema significantly with percent of inhibition 37,37 and32,59%, but supplementation of ethanolic extract of guava stem leather concentration 1% didnot shows inhibition effect .Keywords: guava stem leather, antiinflamation, edema
ANALISIS RHODAMIN B PADA LIPSTIK YANG BEREDAR DI PASAR KOTA MANADO Mamoto, Lidya Valda; Fatimawali, Fatimawali; Citraningtyas, Gayatri
PHARMACON Vol 2, No 2 (2013): pharmacon
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.1689

Abstract

Rhodamin B is a synthetic dye that banned for use in cosmetics and is certified as ahazardous material according to Minister of Health of Indonesian Republic No.376/Menkes/Per/1990 because it cause liver damage, kidney and lymph glands damage,followed with anatomy changes such as organ enlargement. Rhodamin B dye is often usedfor food and beverage products. The study was conducted to identify Rhodamin B in lipstickin the market of Manado City. Samples was taken from 3 main market in Manado city,namely Karombasan market, Pasar 45 market, and Bersehati market. Samples were soakedwith amonia solution, then using wool yarn to extract rhodamin B dye, followed byidentification using thin layer chromatography (TLC) then detected with UV light 254 and366 nm. Rhodamin B was evaluated using UV-Vis spectrophotometry. The result shows that9 examined samples doesn’t contain rhodamin BKeywords : Rhodamin B, Lipstick, thin layer chromatography (TLC), UV-VisSpectrophotometry.
Analisis Kandungan Akrilamida Dalam Pisang Goreng Yang Beredar di Kota Manado Menggunakan Kromatografi Cair Kinerja Tinggi (KCKT) Tandi, Debora; Fatimawali, Fatimawali; Wehantouw, Frenly
PHARMACON Vol 1, No 2 (2012)
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.1.2012.490

Abstract

Acrylamide can be formed as a consequence of heating athigh temperature (more than1200C) in food contains high of carbohydrate. Acrylamide was classified as carcinogeniccompound to human being. Junk food which mostly eaten by people is fries. The objectiveof this research is to identify acrylamide in banana fries marketed in Manado city usingHigh Performance Liquid Chromatography (HPLC). Samples were taken from 12 places,by 6 sub district Manado City. Acrylamide were analyzed by HPLC using Shim-Pack VPODS(4,6 x 250 nm) column, comparison of mobile phase acetonitrile and phospate acid11,45 mM (5:95), flow rate 1 ml/minuteand at wave lenght 210 nm. The result shows thatAcrylamide contains in banana fries with retention time ±4,284 minutes. Acrylamidecontained in banana fries which marketed in Manado City were 39,65 – 1789,52 μg/kg.Acrylamide level in banana fries is at under lethal dose (LD50)Keywords : Acrylamide, banana fries, HPLC, Manado
IDENTIFIKASI DAN PENETAPAN KADAR ASAM BENZOAT PADA KECAP ASIN YANG BEREDAR DI KOTA MANADO Sumarauw, Windy; Fatimawali, Fatimawali; Yudistira, Adithya
PHARMACON Vol 2, No 1 (2013): pharmacon
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.881

Abstract

ABSTRACT   The salty ketchup is one of the food additives commonly added to food. The purpose of this study was to identily and determine levels of use of benzoic acid compound as a preservative in salty ketchup turn in Manado. The samples used were four different ketchup brands produced locally in Manado. Sample identification is done by extracting the sample using diethyl ether solvent. Ether was evaporated to obtain dried ketchup extract. The extracted residue was reacted with 5 % FeCl3 resulting in brown precipitate which proves that it contains benzoic acid. Determination of the preservative (benzoic acid) was determined using UV-Vis spectrophotometer. The results showed that four samples of ketchup used benzoic acid as a preservative. Using preservative not weather threshold set out in Decree PERMENKES RI No 722/MenKes/Per/IX/88.   Keywords : benzoate, ketchup, Manado, UV-Vis spectrophotometer.
Hubungan antara aktivitas olahraga dengan ritme sirkadian dan stres Poluakan, Ricko Johanes; Manampiring, Aaltje E.; Fatimawali, .
Jurnal Biomedik : JBM Vol 12, No 2 (2020): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.12.2.2020.29441

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Abstract: The aims of this study to know the description and the relationship between sports activity, circadian rhythm, and stress. The method used is quantitative research. Sample were determine by the total sampling amounted to 60 in the interests of Occupational Health and Safety in the Faculty of Public Health Sam Ratulangi University class of 2017. Analysis of test data using spearman correlation coefficient. The results of the research is the activity of the sports category was 48.3%, low 20.7%, the category of circadian rhythm less 56.9%, and the category of bad 20.7%. Category of stress was 56.9%, lower 19.1%. Test spearman correlation coefficient found a significant relationship between sports activity with stress, is known to significant (2 tailed) i.e. 0.01 with the level of the strength of the relationship moderates as well as the direction of the relationship positive. Test spearman correlation coefficient between the circadian rhythm of stress found a significant relationship, it is known the value of significant (2 tailed) is 0.000 with the level of the strength of the relationship moderates as well as the direction of the relationship positive. Conclusion in this study is that there is a relationship between sports activity and circadian rhythm with the stress on the students evidenced significant.Keywords: sports activities, circadian rhythm, stress  Abstrak: Penelitian ini bertujuan untuk mengetahui gambaran dan hubungan antara aktivitas olahraga, ritme sirkadian dan stres. Metode yang digunakan yaitu jenis penelitian kuantitatif. Sampel ditentukan secara total sampling berjumlah 60 pada peminatan Kesehatan dan Keselamatan Kerja (K3) di Fakultas Kesehatan Masyarakat (FKM) Universitas Sam Ratulangi Angkatan 2017. Analisis data menggunakan uji koefisien korelasi spearman. Hasil penelitian ialah aktivitas olahraga kategori sedang 48.3%, rendah 20.7%, kategori ritme sirkadian kurang 56.9%, dan kategori buruk 20.7%. Kategori stres sedang 56.9%, rendah 19.1%. Uji koefisien korelasi spearman ditemukan adanya hubungan signifikan antara aktivitas olahraga dengan stres, diketahui nilai signifikan (2 tailed) yaitu 0.01 dengan tingkat kekuatan hubungan moderat serta arah hubungan positif. Uji koefisien korelasi spearman antara ritme sirkadian dengan stres ditemukan adanya hubungan yang signifikan, diketahui nilai signifikan (2 tailed) yaitu 0.000 dengan tingkat kekuatan hubungan moderat serta arah hubungan positif. Simpulan dalam penelitian ini ialah terdapat hubungan antara aktivitas olahraga dan ritme sirkadian dengan stres pada mahasiswa dibuktikan signifikan.  Kata kunci: aktivitas olahraga, ritme sirkadian, stres
Analisis Senyawa Tannin Dan Aktifitas Antibakteri Fraksi Buah Sirih (Piper betle L) Terhadap Streptococcus mutans Makatambah, Venila; Fatimawali, Fatimawali; Rundengan, Gerald
Jurnal MIPA Vol 9, No 2 (2020)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.9.2.2020.28922

Abstract

Kerusakan gigi salah satunya adalah karies gigi yang di sebabkan oleh bakteri Streptococcus mutans. Buah sirih (Piper betle L) dapat di gunakan untuk  tanaman obat karena memiliki kandungan senyawa tanin sebagai antibakteri. Tujuan penelitian untuk mengetahui kandungan senyawa tanin dan uji aktifitas antibakteri fraksi n-heksan, kloroform dan etil asetat terhadap bakteri Streptococcus mutans. Jenis penelitian ini ialah Deskriptif Analitik. Pengujian kandungan senyawa tanin secara kualitatif ekstrak buah sirih menggunakan kromatografi lapis tipis (KLT) dan spektrofotometer Uv-Vis serta pengujian antibakteri pada konsentrasi 3%, 4%, 5% dan 6% untuk masing-masing fraksi. Hasil penelitian menunjukan bahwa ketiga fraksi: n-heksan, kloroform dan etil asetat positif mengandung senyawa tanin. Pengujian antibakteri pada konsentrasi 3%, 4%, 5% dan 6% menunjukan adanya aktifitas antibakteri dari ketiga fraksi : n-heksan ,kloroform dan etil asetat terhadap bakteri Streptococcus mutans dengan daya hambat berturut-turut untuk fraksi n-heksan 12.883±0.510; 12.600±1.455; 13.916±2.877; 13.550±3.347, untuk fraksi kloroform 16.400±1.646; 16.450±1.053; 17.183±1.830; 17.916±1.338 dan fraksi etil asetat 16.400±1.200; 16.000±0.327; 16.850±1.253; 17.450±1.297. Sehingga dapat disimpulkan ekstrak buah sirih mengandung senyawa tanin dan ketiga fraksi n-heksan ,kloroform dan etil asetat memiliki aktifitas antibakteri terhadap Streptococcus mutans dengan kategori kuat.Tooth decay one of which is dental caries caused by Streptococcus mutans bacteria. Betel fruit (Piper betle L) can be used for medicinal plants because it contains tannin compounds as antibacterial. The purpose of this study was to determine the content of tannin compounds and test the antibacterial activity of the n-hexane, chloroform and ethyl acetate fractions against Streptococcus mutans. This type of research is Descriptive Analytic. Testing the content of tannin compounds qualitatively betel fruit extract using thin layer chromatography (TLC) and Uv-Vis spectrophotometer and antibacterial testing at concentrations of 3%, 4%, 5% and 6% for each fraction. The results showed that all three fractions: n-hexane, chloroform and ethyl acetate positively contained tannin compounds. Antibacterial testing at concentrations of 3%, 4%, 5% and 6% showed antibacterial activity of the three fractions: n-hexane, chloroform and ethyl acetate against Streptococcus mutans with inhibitions for the n-hexane fraction 12.883 ± 0.510; 12,600 ± 1,455; 13,916 ± 2,877; 13,550 ± 3,347, for the chloroform fraction 16,400 ± 1,646; 16,450 ± 1,053; 17,183 ± 1,830; 17,916 ± 1,338 and the ethyl acetate fraction 16,400 ± 1,200; 16,000 ± 0.327; 16,850 ± 1,253; 17,450 ± 1,297. So it can be concluded that betel fruit extract contains tannin compounds and the three fractions of n-hexane, chloroform and ethyl acetate have antibacterial activity against Streptococcus mutans with a strong category.
Molecular Docking Senyawa Gingerol dan Zingiberol pada Tanaman Jahe sebagai Penanganan COVID-19 Ratu, Belinda D. P. M.; Bodhi, Widdhi; Budiarso, Fona; Kepel, Billy J.; Fatimawali, .; Manampiring, Aaltje
eBiomedik Vol 9, No 1 (2021): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.9.1.2021.32361

Abstract

Abstract: COVID-19 is a new disease. Many people feel the impact of this disease. There is no definite cure for COVID-19, so many people use traditional medicine to ward off COVID-19, including ginger. This study aims to determine whether there is an interaction between compounds in ginger (gingerol and zingiberol) and the COVID-19’s main protease (6LU7). This study uses a molecular docking method using 4 main applications, namely Autodock Tools, Autodock Vina, Biovia Discovery Studio 2020, and Open Babel GUI. The samples used were gingerol and zingiberol compounds in ginger plants downloaded from Pubchem. The data used in this study used Mendeley, Clinical Key, and PubMed database. The study showed that almost all of the amino acid residues in the gingerol compound acted on the 6LU7 active site, whereas the zingiberol did not. The results of the binding affinity of ginger compounds, both gingerol and zingiberol, do not exceed the binding affinity of remdesivir, a drug that is widely researched as a COVID-19 handling drug. In conclusion, gingerol and zingiberol compounds in ginger can’t be considered as COVID-19’s treatment.Keywords: molecular docking, gingerol, zingiberol Abstrak: COVID-19 merupakan sebuah penyakit yang baru. Banyak masyarakat yang merasakan dampak dari penyakit ini. Belum ada pengobatan pasti untuk menyembuhkan COVID-19, sehingga banyak masyarakat yang menggunakan pengobatan tradisional untuk menangkal COVID-19, termasuk jahe. Penelitian ini bertujuan untuk mengetahui apakah ada interaksi antara senyawa pada jahe (gingerol dan zingiberol) dengan main protease COVID-19 (6LU7). Penelitian ini menggunakan metode molecular docking dengan menggunakan 4 aplikasi utama, yaitu Autodock Tools, Autodock Vina, Biovia Discovery Studio 2020, dan Open Babel GUI. Sampel yang digunakan yaitu senyawa gingerol dan zingiberol pada tanaman jahe yang diunduh di Pubchem. Data yang digunakan dalam penelitian ini menggunakan database Mendeley, Clinical Key, dan PubMed. Penelitian menunjukkan bahwa hampir semua residu asam amino pada senyawa gingerol bekerja pada sisi aktif 6LU7, sedangkan tidak demikian pada zingiberol. Hasil binding affinity senyawa jahe, baik gingerol maupun zingiberol tidak  melebihi binding affinity remdesivir, obat yang banyak diteliti sebagai obat penanganan COVID-19. Sebagai simpulan, senyawa gingerol dan zingiberol pada tanaman jahe tidak dapat dipertimbangkan sebagai penanganan COVID-19Kata Kunci: molecular docking, gingerol, zingiberol
Molecular Docking terhadap Senyawa Isoeleutherin dan Isoeleutherol sebagai Penghambat Pertumbuhan SARS-CoV-2 Prasetio, Nathanael F.; Kepel, Billy J.; Bodhi, Widdhi; Fatimawali, .; Manampiring, Aaltje; Budiarso, Fona
e-Biomedik Vol 9, No 1 (2021): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.v9i1.31809

Abstract

Abstract: Dayak onions are herbal plants used by Indonesians as an anti-inflammatory, anticancer, antimicrobial, antidiabetic, antihypertensive and antiviral. By consuming herbal plants, it can increase immunity which is the main key in preventing the virus, especially COVID-19. This study was aimed to determine the effect of dayak active compound anchoring on the growth of the corona virus. This study used molecular docking method. The results of visualization of molecular docking when compared to the binding affinity of remdesivir obtained a binding affinity of -7.3, while the binding affinity of isoeleutherin and isoeleutherol was -6.9, so the result was lower than remdesivir. isoeleutherin and isoeleutherol compounds have a lower binding affinity value than remdesivir, so isoeleutherin and isoeleutherol compounds have lower yields as inhibitors of COVID-19.Keywords: dayak onions, SARS-CoV-2, molecular docking  Abstrak: Bawang Dayak merupakan tanaman herbal yang digunakan masyarakat Indonesia sebagai antiinflamasi, antikanker, antimikroba, antidiabetes, antihipertensi dan antivirus. Dengan mengkonsumsi tanaman herbal dapat meningkatkan kekebalan tubuh yang menjadi kunci utama dalam mencegah virus terlebih COVID-19. Penelitian ini bertujuan untuk mengetahui pengaruh penambatan senyawa aktif bawang dayak terhadap pertumbuhan dari corona virus. Jenis penelitian ini menggunakan metode penelitian penambatan molekul (molecular docking). Hasil visualisasi molecular docking jika dibandingkan binding affinity dari remdesivir didapatkan binding affinity yaitu -7,3 sedangkan binding affinity dari isoeleutherin dan isoeleutherol adalah -6,9, maka diperoleh hasil yang lebih rendah dari remdesivir. senyawa isoeleutherine dan isoeleutherol memiliki nilai binding affinity yang lebih rendah dari remdesivir, maka senyawa isoeleutherin dan isoeleutherol memiliki hasil yang lebih rendah sebagai penghambat COVID-19.Kata Kunci: bawang dayak, SARS-CoV-2, molecular docking
Co-Authors Aaltje E. Manampiring Aaltje E. Manampiring Aaltje E. Manampiring Aaltje Ellen Manampiring Aaltje Manampiring Aaltje Manampiring Aaltje Manampiring Aaltje Manampiring Abas, Abdul Hawil Abdul Jalil Abubakar, Poetry M.S. Abubakar, Poetry Melinda Achmad, Mardhita Nilamsari Adeanne Wullur Adikila, Gregorius Giani Adithya Yudistira Adrian Umboh Ahmad Haekal Alamri, Firdaus Alexander S. L. Bolang Andini Andrew Pangemanan, Andrew Angelina Stevany Regina Masengi Angelique Baya, Gloria Prisca Susana Angelique C. M. Tanan Anggelia Nelisa Kapantow Anita Linda Yohana Pratasik Anita Mamuaya Anita Rahel Meilina Paendong Anjely Jessica Makatempuge Annisa Hamka Antasionasti, Irma Apolonia Amelintje Gusmão Awoah Asriyana S Kaseng Astrid Noviera Iksan Axl L. Windah Ayu Fauzia Malinda B H. R. Kairupan Baharuddin, Fatmawaty Bahter, Julia V.F. Barasarathi , Jayanthi Basalamah, Rizqah BEIVY JONATHAN KOLONDAM Bernadus, Zefanya Bernadus, Zefanya G Billy J Kepel Billy J Kepel Billy J. Kepel Billy J. Kepel Billy Johnson Kepe Billy Kepel Billy Kepel Billy Kepel Bodhi , Widdhi Budiarso, Fona Hermina Dwiana Budiarso, Fone D.H. Budiarso, Hermina Butue, Leobernard Catur Riany Celik, Ismail Changiz Mohiyeddini Cheisy Anastasya Gratia Lengkong Chintya G. Derek Christi Diana Mambo Damaris Damaris Datu, Olvie S. Datu, Olvie Syenni Debbie Retnoningrum Debora Tandi DEBY AFRIANI MPILA Defny S. Wewengkang, Defny S. Deliyana Lanti Desye Nurmalita Tanan Devianitta Sarapi Dian Pratiwi Dina V. Rombot Dinda V. Lomotu Dolongtelide, Jeclin Inebel Donald Andreas Chandra Dos D. L. E. Br Siagian Edward Nangoy Eka Mulya Muthalib Ekawati Tallei, Trina Ellen Manampiring, Aaltje Elly Suoth Endang Triastuti Engka, Joice Nancy Ermawati Ermawati Eva M. Mantjoro Farhan A. Bidullah Fatima Fatima Fatmawaty Badaruddin Febrisa Salsabila Feti Fatimah Feybe Ireine Kumayas Fona Budiarso Frans S. M. Mintardjo Frederika Filly Toad Frenly Wehantouw Frisky S. Badi, Frisky S. Friyan Criscanus Manopo Gabriela Clementine Ranti Gani Jumaat Gani, Maria A Gani, Maria A. Gayatri Citraningtyas Gazali Gazali Gemi Nastiti Gerungan, Yizreel Y. GONI, BRIGITA CHRISTANIA AURORA Grenshannya Anasthasya Pua Greta J. P. Wahongan Gueen L. G. Naray Gustaaf A. E. Ratag Hamdiyah Hamzah Hamidah Sri Supriati Hanna Elga Sulu Hariyanto, Yuanita Amalia Hasan, Puput Herawati Hasan, Puput Herawati Said Hasanuddin, Israyati R. Hebber, Tri Heedy M Tjitrosantoso Henki Rotinsulu, Henki Hermina Budiarso, Fona Dwiana Herny E.I. Simbala Hosea Jaya Edy I Dewa Ayu Accyuta Kirana I Dewa Ayu Prabawati I Putu Andika Arianta Inda V. M. Rawung, Inda V. M. Intan Baiduri Inti Rizcy Sari Sugeha Irawan Yusuf Irawan Yusuf Irma Antasionasti Irma Antasionasti Irma Antasionasti Irma Febrianti Wahongan Irma Tristanti Iswanto Toling Jainer P Siampa Jainer Pasca Siampa Jayanto, Imam Jeane Mongi Jimmy Posangi Jimmy Posangi Jimmy Posangi Jimmy Posangi Jimmy Posangi John Socrates Kekenusa John Socrates Kekenusa Johnson Kepel, Billy Josepin P Konda Julianri Lebang Julianri Sari Lebang Julianri Sari Lebang Junita M. Pertiwi Junita Maja Pertiwi Jurike Kaunang Kairupan, Tara S. Kalalo, Marko Jeremia Kalalo, Tekla Kamaruddin Kamaruddin Kambey, Debora F. Kapondo, Gwendolyn L. Karamoy, Eunike M. Kawengian, Shirley ES. Keintjem, Brigieta Kepel, Billy Kepel, Billy Johnson Kepel, Regina E.M. Kezia Pangemanan Kissi Parengkuan Konda, Josepin P Kuliahana, Ana Kumowal, Selphina Lady zha-zha Luntungan Lampus, Christania Landy A. Ch Lolaen LAPIAN, ALDA JULISTY GABRIELA Lasut, Misella R. C. Lasut, Misella Regina Laurencia Diva Bernadette Artantyo Lengkong, Cheisy Anastasya Gratia Leonardo Delvin Gosal Ley, Gabriella Therezia Ley Lidya Togelang Lidya Valda Mamoto Lisa Kepel M. Fadhel Nurmidin Ma'ruf, Nurul Qalbiyyah Makatambah, Venila Malino, Angeline Priscillia Mambo, Christi Diana Manampiring, Aaltje Ellen Mansur, Nurfauziah Mantjoro, Eva M. Maria Immaculata Tangkau Marina Mamarimbing Marko Jeremia Kalalo Marko Jeremia Kalalo Maulydia, Nur B. Max R.J. Runtuwene Meilani Jayanti Melkianus, Brando Miranti Aike Wemay Mohtar, Khumairah Monalisa Karinda Morgen Ekaputra Pangaila Muh. Rifaldi Latarang Muharli Qadri Kanon Mutiara Cantika Mutiara Ramadani Mutmainah Mutmainah Nainggolan, Ivana C. Natasya Ester Rebeca Tamahiwu Natsir, Rosdiana Ni Wayan Dianti Niode, Nurdjannah Jane Nova Hellen Kapantow Novelina Irianti Damanik Nurdjannah J. Niode Nurfitriani Nurfitriani, Nurfitriani Nurhamidin, Anastasia P.R. Oksfriani Jufri Sumampouw Olivia C. Mulalinda Olivia G. Mokolensang, Olivia G. Olvie Datu Olvie Syenni Datu Olvie Syenni Datu Olvie Syennie Datu Omega Agral Owu, Nadya M. PANI, PUTRI MARGARETHA GLAUDY Pantow, Natalia M. Patrick Muljono Patrick Muljono, Patrick Paukiran, Degol Paulina Yamlean Paulina yamlean Paulina Yamlean Pehino, Albrita Pendong, Christa Hana Angle Poluakan, Ricko Johanes Prasetio, Nathanael F. Prayoga, Deshanda Kurniawan Puasa, Novanda S. Purwanto, Diana Shintawati Putri, Ida A. R. Putri, Prisna Aswarita Rahmad Dian Rambi, Christani I J Ratte, Titah Amelia Ratu, Belinda D. P. M. Ratulangi, Andrew Johanes Refa Riski Aulia Rengur, Zul Aini Rifky Aditya Rahadian Rifqi Adithya Rika Wulandari Rimporok, Aprilia Rinaldi Idroes RINI Rio Windy Sondakh Ristanti Pratiwi Rizya Marchilia Mamahit Rotinsulu, Sarah Rotty, Linda WA. RUMALUTUR, CHRISTHALIA IEWANDA Rumangu, Chrisa P. Rumondor, Erladys M. Rundengan, Gerald Sahi, Makbul Renaldi Saragih, Nathin Loria , Sarah Mariana Pattuju Saranita V. G. Polii sari sakinah Sherly Dawile Shintia, Arini Siampa, Jainer P Siampa, Jainer Pasca Siboantua Broolin Simanjuntak Siringo-Ringo, Aurian Fricilia Siti Suhartini Sitti Romlah Sri Sudewi Sri Sudewi, Sri Sriwaningsih S Nahe Stevana F.A. Paat Sumakul, Gilbert Samuel Sumual, Acika Surya, Welong S. Syifa Katili Talla, Tamrin Tallei, Trina Ekawati Tania, Adinda Dwi Tendean, Lydia Estelina Naomi Terence Kanzil Titah Amelia Ratte Tjandra A. Rumagit Tjandra, Regina F. Trina E Tallei Trina E Tallei Trina E. Tallei, Trina E. Trina Ekawati Tallei Trina Ekawati Tallei TRINA EKAWATI TALLEI Trina Tallei Trina Tallei Trina Tallei Turalaki, Grace Lendawati Amelia Vanessa J. T. Seran, Vanessa J. T. Vibrila Berliana Djanas Watania, Marcela Wawo, Arsianita Ester Welong S. Surya Welong S. Surya Welong S. Surya Weny I Wiyono Weny Wiyono Wewengkang, Defny Widdhi Bodhi Widdhi Bodhi Widdhi Bodhi Widdhi Bodhi widdhi Bodhi Widdhi Bodhi Widhi Bodhi Widya Hana Putri Gerung Wijaya, Puspita Windy Sumarauw Wulan P. J. Kaunang Wulan Palilingan, Wulan Wulan Tuerah Wungouw, Herlina Ineke Surjane Wungouw, Herlina IS. Yohanes Adithya Koirewoa Yulia Maria Jarut Yulianty Sanggelorang Yuyun Andriani Agus Zahra Zahra Zainal Abidin Zulfikri Zulfikri, Zulfikri