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Journal : Jurnal Kedokteran Hewan

PENGARUH EKSTRAK ETANOL DAUN JALOH (Salix Tetrasperma Roxb) TERHADAP PERSENTASE PARASITEMIA PADA MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium berghei Nuzul Asmilia; Sugito Sugito; Erdiansyah Rahmi; Niko Febrianto
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.86 KB) | DOI: 10.21157/j.ked.hewan.v4i1.9796

Abstract

This study aimed to verify the effect of ethanol extract of jaloh leaves at various doses on parasitemia percentage of in mice (Mus musculus), wich inoculated Plasmodium berghei. This study used 12 male mice (Mus musculus) strains Balb / C with average body weight of 30.2 grams of 2-month-old. This research using Completely Randomized Design (CRD) with four treatments and three replications. All the mice were infected with Plasmodium berghei prior to treatment on three days post infected the mice in group 2,3 and 4 were given with extract ethanol jaloh leaves at dose 50, 100, 150 mg/kgbw inspectively. The mice in group 1 (K0) were not given anything and consident as control.Observations parasitemia percentage using microscopy on preparations stained thin blood smear Giemsa. The parasitemia percentage data were analyzed using one way ANOVA. On the average (±SD) percentage of parasitemia during the three days in four treatments (K0, K1, K2, and K3) on the first day were 24.87±1.86; 24.33±1.53; 24.20±1.06; and 24.93±1.01. On the second day were 22.67±2.52; 23.00±1.00; 22.80±1.20; and 24.13±2.01; and on the third day were 24.77±1.66; 22.97±3.76; 21.13±1.90; and 24.27±1.42. Statistical analysis showed that ethanol extract of the jaloh leaves for KO, KI, KII, and KIV respectively treatment did not affect the percentage of parasitemia in mice (Mus musculus) inoculated Plasmodium berghei.
The Distributions of Glycogen in the Stomach on Long Tailed Macaque (Macaca fascicularis) during Pre and Postnatal Period Erdiansyah Rahmi
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2447.781 KB) | DOI: 10.21157/j.ked.hewan.v2i2.9785

Abstract

The study was done to investigate the glycogen distribution in the stomach of long tailed macaques (Macaca fascicularis). The study used eight individuals of long tailed macaques consist of 6 fetuses (55, 70, 85, 100, 120, and 150 days pregnancy) and 2 infants (10 and 105 days). The gastrict tract were histologically stained using Hematoxyllin-Eosin (HE), and histochemically stained using Periodic Acid Schiff (PAS), to detect glycogen. The results revealed that the histogenesis pattern of stomach glandullar cells are developed in line with the age of the fetuses and infant. We also found that glycogen existance are increase in line with the age in the glandullar stomach. From the present study we concluded that the glycogen distribution in gastrict glandular cells were influenced by the age in the fetal period, while in the infant was under influence of the digestion activities, and the activities of the gastrict glandular cells was already seen at 55 days gestation.
INHIBIN B MENURUNKAN KONSENTRASI FOLLICLE STIMULATING HORMONE (FSH) PADA TIKUS PUTIH (Rattus norvegicus): UPAYA PENGEMBANGAN KONTRASEPSI HORMON PRIA BERBASIS PEPTIDA Muslim Akmal; Aulanni’am A; M. Aris Widodo; Sutiman B. Sumitro; Basuki B. Purnomo; Tongku Nizwan Siregar; Muhammad Hambal; Amiruddin A; Syafruddin S; Dwinna Aliza; Arman Sayuti; Mulyadi Adam; T. Armansyah; Erdiansyah Rahmi
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.745 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2788

Abstract

Penelitian ini bertujuan mengetahui efek injeksi inhibin B terhadap penurunan konsentrasi follicle stimulating hormone (FSH) di dalamserum pada tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 24 ekor tikus putih berjenis kelamin jantan dengan strain Wistar berumur 4 bulan dengan bobot badan 150-200 g. Tikus-tikus dikelompokkan secara acak ke dalam 4 kelompok, yaitu KK0, KP1, KP2, dan KP3, masing-masing kelompok terdiri atas 6 ekor. Kelompok KK0 merupakan kelompok kontrol hanya diinjeksi dengan phosphate buffer saline (PBS), sedangkan kelompok KP1, KP2, dan KP3 diinjeksi dengan inhibin B dengan dosis berturut-turut 25, 50, dan 100 pg/ekor. Injeksi inhibin B dilakukan secara intraperitoneum sebanyak 5 kali selama 48 hari dengan interval waktu 12 hari. Injeksi pertama inhibin B dilarutkan dengan0,05 ml PBS dan 0,05 ml Freud’s complete adjuvant (FCA). Injeksi kedua sampai kelima, inhibin B dilarutkan dengan 0,05 ml PBS dan 0,05 ml Freud’s incomplete adjuvant (FICA). Pada hari ke-6 setelah injeksi inhibin B terakhir, tikus dikorbankan secara dislocatio cervicalis,lalu darah dikoleksi langsung dari jantung dan didiamkan hingga didapatkan serum untuk pemeriksaan konsentrasi FSH dengan menggunakan metode enzyme-linked immunosorbent assay (ELISA). Hasil penelitian menunjukkan bahwa injeksi inhibin B dengan dosis 100 pg/ekor menurunkan konsentrasi FSH secara nyata (P0,05) bila dibandingkan dengan kelompok kontrol. Berdasarkan hal tersebut, inhibin B berpeluang untuk dikembangkan sebagai kandidat kontrasepsi pria hormon berbasis peptida.
The Effect of Caffeine Treatment during Organogenesis Period on the Birth Weight of the Rat Fetuses (Rattus norvegicus) Hery Wijayanto; Tri Wahyu Pangestiningsih; Erdiansyah Rahmi
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.818 KB) | DOI: 10.21157/j.ked.hewan.v1i2.3123

Abstract

The study was conducted to investigate the effects of caffeine treatment during organogenesis period to the fetal birth weight, using rat (Rattus norvegicus) as the animal model. Thirty-six primipararat obtained from Unit Pengembangan Hewan Percobaan, Gadjah Mada University (UPHP-GMU), 3 month old, 165-200 g body weight, were divided into 6 groups, consisted of 6 rats each. Six of the ratshave been selected based on the estrous cycles, and only rat with regular estrous were use for theexperiment. The rat then were mated, and during day 6-14 of the pregnancies were treated orally withcaffeine diluted in aquadest in dosage: placebo (1 cc aquadest) for group I (control), and 5.4, 10.8, 16.2,21.6, and 27 g/200 g body weight/day for treatment groups II-VI respectively. The pregnant rat bodyweights were determined at day 6 of pregnancies for calculating the caffeine treatment dosages. At day 20thof the pregnancies all of the pregnant rats were caesarotomized, and all of the fetuses were removed and weighed. The results showed that all of the treatment groups have significantly lower birth weightcompare to the groups control group. More over, fetal obtained from the treatment groups also showedserious subcutaneous hemorrhagic.Keywords: organogenesis, Rattus norvegicus, birth weight
CORRELATION BETWEEN PROGESTERONE HORMONE CONCENTRATION AND NUMBER OF FETUSES OF ETAWA CROSSBRED GOAT IN DIFFERENT GESTATION PERIOD Syafruddin Syafruddin; Khairul Rizal; Rosmaidar Rosmaidar; Juli Melia; roslizawaty roslizawaty; Zuhrawati Zuhrawati; Erdiansyah Rahmi; Mahdi Abrar
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.248 KB) | DOI: 10.21157/j.ked.hewan.v11i3.8687

Abstract

This study aimed to investigate the correlation between progesterone hormone concentration and number of fetus as well as the difference in progesterone hormone concentration from different gestation periods in Etawah crossbred (EC). Five EC goats used in this study were injected with 2 mL of 5.5 mg prostaglandin F2α (PGF2α, Capriglandin) intramuscularly, with an interval of 10 days. Goats that showed symptoms of estrus were mated naturally with male goats. Blood samples were taken on the 7th, 14th, 21st, and 75th days of gestation. Progesterone hormone concentration was determined using enzyme linked immunosorbent assay (ELISA). The number of fetus was determined at 35th gestational day using ultrasonography (USG). Four out of 5 goats were found to have 1 fetus and 1 goat had 2 fetuses. The progesterone concentration on the 7th, 14th, 21st, and 75th days of gestation in goats with single fetus were 5.21, 7.78, 11.97, and 18.78 ng/mL, respectively, while in goat with two fetuses were 8.44, 14.53, 16.81, and 22.73 ng/mL, respectively. The correlation (r) between progesterone hormone concentration and number of fetus on the 7th, 14th, 21st, and 75th days of gestation were 0.442, 0.854, 0.592, and 0.757, respectively. It is concluded that progesterone concentrations are highly correlated to the number of fetus in each gestation period in EC goats.
RESPONS HSP-70 DAN KADAR KORTISOL AKIBAT PEMBERIAN KOMBINASI EKSTRAK JALOH DAN KROMIUM PADA AYAM BROILER YANG MENGALAMI CEKAMAN PANAS Sugito s; Erdiansyah Rahmi; Muhammad Isa
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.518 KB) | DOI: 10.21157/j.ked.hewan.v6i2.341

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian kombinasi ekstrak jaloh dan kromium terhadap pembentukan heat shock protein-70 (HSP-70) dan kadar kortisol ayam yang mengalami cekaman panas. Dalam penelitian ini digunakan ayam broiler sebanyak 12 ekor yang dibagi atas empat perlakuan: Perlakuan I (EC), cekaman panas + dan kombinasi 1.000 mg ekstrak jaloh dengan 1.000 μg kromium per liter air minum, perlakuan II (E), cekaman panas + 1.000 mg ekstrak jaloh per liter air minum, perlakuan III (KD), cekaman panas + 0,0 mg ekstrak jaloh dan 0,0 μg kromium, dan Perlakuan IV (KL), tanpa cekaman panas dan 0,0 mg ekstrak jaloh dan 0,0 μg kromium. Masing-masing perlakuan terdiri atas tiga ulangan. Metode pemberian cekaman panas dilakukan dengan meningkatkan suhu dalam kandang pada 33±1° C selama lima jam per hari dalam waktu lima belas hari. Pemberian kombinasi ekstrak jaloh dan kromium dilakukan dengan cara melarutkannya dalam air minum dan diberikan selama dua jam (pemberian pukul 10.00) sebelum suhu di dalam kandang mencapai 33±1° C. Pengambilan sampel serum dan jaringan paru dilakukan pada hari ke-15 pelaksanaan penelitian (ayam umur 36 hari). Pengambilan sampel darah dilakukan sebelum ayam dipotong dan jaringan organ paru diambil setelah ayam dipotong. Pada sampel serum dilakukan pemeriksaan kortisol dengan enzymelinkedimmunosorbantassay (ELISA) dan deteksi HSP-70 di dalam jaringan paru dilakukan menggunakan metode imunohistokimia. Hasil penelitian ini menunjukkan bahwa pemberian cekaman panas pada suhu 33±1° C selama lima jam per hari dapat meningkatkan pembentukan HSP-70 dalam paru dan kadar kortisol dalam serum. Pemberian ekstrak jaloh secara tunggal lebih efektif menurunkan jumlah HSP-70 pada jaringan paru dibandingkan jika dikombinasi dengan kromium.
Morphological on the Macroscropic Development of the Stomach in the Long-tailed Macaque (Macaca fascicularis) during Pre Natal and Post Natal Period Erdiansyah Rahmi
Jurnal Kedokteran Hewan Vol 1, No 1 (2007): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1537.595 KB) | DOI: 10.21157/j.ked.hewan.v1i1.3119

Abstract

This research was conducted with aim to reveal the morphological changes on the stomach in thelong-tailed macaque (Macaca fascicularis) (Mf) during prenatal and postnatal development. Stomachtaken from seven fetuses (40, 55, 70, 85, 100, 120, and 150 gestation day) and two newborns (10 and 105day old) animals was used in this study. The stomach was studied at macroscopic for observation of thegeneral structure. Measurement conducted to stomach length, wide of stomach body, greater curvature,lesser curvature of the stomach in each of old age group. Perception result indicate that the stomach of Mf was red bean like in form with short minor kurvatura. These values increased along with the age of fetusand infant. The Lesser curvature very short. Greater curvature relative length (twice improvement) atpost natal (infant) compared to before birth (fetus). The morphological development of the stomachincreased with age, with very rapid further development after birth, which is possibly due to the age of thefetus and in addition, the start of digestive activity and optimalization of stomach function.Keywords: anatomy, digestion, primates
THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO Nuzul Asmilia; T Armansyah TR; Dwinna Aliza; Juli Melia; Erdiansyah Rahmi; Lingga Surya Maret Daulay
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.356 KB) | DOI: 10.21157/j.ked.hewan.v12i4.10215

Abstract

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.
EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT Aulia, Usma; Wahyuni, Sri; Gholib, Gholib; Dasrul, Dasrul; Adam, Mulyadi; Rahmi, Erdiansyah; Hamzah, Abdullah
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i1.30042

Abstract

The objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW.
MICROSTRUCTURAL CHARACTERISTICS OF THE Longissimus dorsi MUSCLE IN BRAHMAN CROSS CATTLE Sofyan, Hamny; Jalaluddin, Muhammad; Rahmi, Erdiansyah; Wahyuni, Sri; Hidayat, Taufan; Adam, Mulyadi; Gani, Fadli A; Sabri, Mustafa; Ritonga, Mudhita Zikkrullah
Jurnal Kedokteran Hewan Vol 16, No 2 (2022): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i2.25885

Abstract

This study aimed to analyze the muscle microstructure of longissimus dorsi Brahman cross (BX) cattle slaughtered at the slaughterhouse of North Aceh District. The meat samples for the study were taken from three male BX cattle aged 18-30 months with body weights ranging from 400 kg to 500 kg and the average body condition scores (BCS) of 3-4. The muscle used was the longissimus dorsi muscle. The mean (SE) diameter and cross-sectional area (CSA) of muscle fibers and the percentage of fast fibers in the longissimus dorsi BX muscle were 71.691.23 m, 4263.43305.69 m2 , and 48.178.81%, respectively. This study concluded that the longissimus dorsi muscle of the BX has relatively large muscle fibers, some of which in each fascicle are composed of fast muscle (fast fiber/type II).
Co-Authors A, Aulanniam A.A. Ketut Agung Cahyawan W Abdullah Hamzah Abdullah Hamzah, Abdullah Al Azhar Ali Murtala Ali, Ihfan Khaidir Amalia Sutriana Amiruddin . Amiruddin A Amiruddin Amiruddin Arman Sayuti Arman Sayuti Aulanni'am, Aulanni'am Aulia, Usma Awaluddin Awaluddin Azhari Azhari B. Tri Wibowo Bagus Dwijayanti Basuki B. Purnomo Basuki B. Purnomo budianto panjaitan Cut Dahlia Iskandar Cut Nila Thasmi Darmawi Darmawi Dasrul Dasrul Daulay, Lingga Surya Maret Dian Masyita Dian Masyitha Dian Masyitha Dian Masyitha Dina Agustina Dwinna Aliza Dwinna Aliza Dwinna Aliza Erina Erina Fachreza Oktavian Syahputri Fadli A. Gani Faisal Jamin Faisal Mustafa Fakhrurrazi Fakhrurrazi Febrianto, Niko Fitriani Fitriani Gani, Fadli A Gholib Gholib Gholib Gholib, Gholib Ginta Riady Habiburrahman Habiburrahman, Habiburrahman Hafizuddin Hafizuddin Hamdan Hamdan Hamny Hamny Hamny Sofyan Henni Vanda Herialfian Herialfian Hery Wijayanto Idawati Nasution Idawati Nasution Intan Regina Iqbar Iqbar Ismail Ismail J. Susilo Juli Melia Juli Melia Khairul Rizal Kusdiantoro Mohamad Lian Varis Riandi Lingga Surya Maret Daulay M Jalaluddin M Nur Salim M Nur Salim M. Aris Widodo M. Aris Widodo M. Hasan M. Isa M. Isa Mahdi Abrar Mardiana Mardiana Meuthya Sr Muhammad Hambal Muhammad Hanafiah Muhammad Isa Muhammad Isa Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Mulyadi Adam Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Mustafa Sabri NA, Zuhrawaty Niko Febrianto Ningrum, Aulia Putri Wahyu Nofri Alfi NURLIANA NURLIANA Nursalim, M Nuzul Asmilia Nuzul Asmilia Putri Aisyah Hanifah Putri Dewi Qathrinnada Ramadhana Rahmat Nazif Ramadhan, Rezky Razali Razali Reza Perdana Putra Rinidar Rinidar Rita Andini Ritonga, Mudhita Zikkrullah Ritonga, Mudhita Zikrullah Rizal, Khairul roslizawaty roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Rusli Rusli Rusli Sulaiman Safika S, Safika Sarayulis Sarayulis Sembiring, Rinawati Siska Pratiwi Siswandy Siswandy Siti Aisyah Siti Hajar Sri Wahyuni Sri Wahyuni Sugito - Sugito Sugito Sultan Fadhilla Taqwa Sutiman B. Sumitro Syafruddin s Syafruddin S Syafruddin Syafruddin Syafruddin Syafruddin T Armansyah TR T. Armansyah T. Armansyah T. Armansyah TR T. Fadrial Karmil T. Reza Ferasyi Tanjung, Fitri Aisah Taufan Hidayat Teuku Reza Ferasyi Tongku Nizwan Siregar TR, T Armansyah Tri Wahyu Pangestiningsih Triva Murtina Lubis Ummu Balqis Wahono Esthi Prasetyaningtyas Wahyuni Azani Wardhani, Lailia Dwi Kusuma Wenny Novita Sari Wisnu Nurcahyo Y. Tinasari Yandi Syahputra Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin, Zainuddin Zico, Hanifa Zuhrawati Zuhrawati Zuhrawati Zuhrawati, Zuhrawati Zuhrawaty Zuhrawaty Zulkifli, Baidillah