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Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Bidara Ziziphus Spina-Christi Dan Uji Aktivitas Antibakteri Terhadap Bakteri Staphylococcus Aureus Cut Dian Mala Luthfia; Dikki Miswanda; Haris Munandar Nasution; Minda Sari Lubis
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 1 (2024): Januari : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i1.168

Abstract

Nanotechnology is currently growing rapidly due to its broad application in science and technology. Nanotechnology itself is a science based on nanoparticles, so a more environmentally friendly method is being developed, namely the "green synthesis" method using plant extracts as a bioreductor. Silver nanoparticles have been synthesized using extracts of bidara leaf as a reducing agent. The objective of this research was was to determine the synthesis of silver nanoparticles using bioreductors and their application as antibacterial agents using bidara leaf extract against Staphylococcus aureus bacteria. This research used the green synthesis method to produce silver nanoparticles. The bidara leaf extract used was divided into several concentrations, namely 1 mM, 2 mM, 3 mM and 4 mM. To characterize silver nanoparticles, UV-Vis and PSA spectrophotometers were used. Furthermore, the Kirby-Bauer method was used to test the antibacterial properties of silver nanoparticles against Staphylococcus aureus bacteria. The results of research that has been done show that the formation of silver nanoparticles is characterized by the emergence of maximum absorbance peaks in the wavelength range of 400-450 nm, while the PSA results at concentrations of 3 and 4µm showed nanoparticle sizes of 185 nm. Silver nanoparticles of bidara leaf extract with various concentrations of 1 mM, 2 mM, 3 mM and 4 mM have antibacterial activity that can inhibit the growth of Staphylococcus aureus bacteria with an average inhibition zone diameter of 13.0617 mm, 11.7333 mm, 9.05 mm and 13.19 mm. This shows that silver nanoparticles with bidara leaf extract have antibacterial properties.
Uji Aktivitas Antibakteri Sabun Transparan Putik Saffron (Crocus sativus L) terhadap Bakteri Staphylococcus aureus Emmy Wiriandini; Gabena Indrayani Dalimunthe; Minda Sari Lubis; Haris Munandar Nasution
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 2 (2024): March : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i2.343

Abstract

Transparent soap is a variation of solid bar soap that not only functions to clean dirt but also has a scrubbing function. Transparent solid soap is one of the soap innovations that has become an attractive soap. Saffron also has antibacterial properties due to the presence of flavonoids, tannins and saponins. Flavonoids are compounds that can have anti-inflammatory, anticancer and antioxidant effects, because they can inhibit the formation of free radicals. It has been used traditionally to treat various ailments including depression, cardiovascular disease, menstrual disorders, asthma, insomnia, digestive ailments, bone pain and several other ailments. Saffron pistil juice is made by dissolving in CO₂-free distilled water. Phytochemical screening was carried out on saffron pistil extract. Saffron pistil juice is made in various soap formulas, namely formula I, formula II and formula III. The positive control used was chloramphenicol antibiotic and the negative control was DMSO. There were several tests carried out on Saffron pistil extract in addition to phytochemical screening, namely antibacterial testing on transparent soap with Saffron pistil extract as a soap additive. The results of phytochemical screening showed that the pistil of saffron (Crocus sativus L) contained secondary metabolites, namely: flavonoids, alkaloids, tannins, and saponins. And for the results of the antibacterial activity research also showed that the Saffron pistil extract against transparent soap which can be used as a soap additive, the antibacterial results had a strong inhibition at the average value of formula III which was 20.5 and formula II was 18.4 mm and formula I 16.1 mm, against Staphylococcus aureus bacteria.
Penetapan Kadar Vitamin C dalam Sari Buah Nanas (Ananas comosus L. MERR) dengan Metode Spektrofotometri UV Maya Syafira; Anny Sartika Daulay; Ridwanto Ridwanto; Haris Munandar Nasution
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 2 (2024): March : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i2.345

Abstract

Pineapple or which has the Latin name Ananas Comosus L. MERR has two main ingredients, namely vitamin C and manganese. Vitamin C is very important for the growth and development of the immune system and can help the absorption of iron from food, meanwhile manganese is a natural mineral that has antioxidant properties or can ward off evil compounds in the body and help increase the body's immunity to avoid the Covid-19 disease outbreak.. Therefore, researchers are interested in researching pineapple juice, because what people consume every day is pineapple juice. The aim of this research was to determine the levels of vitamin C in pineapple juice (Ananas comosus L. MERR) and whether the vitamin C content in pineapple juice (Ananas comosus L. MERR) can meet daily nutritional adequacy rates. This research method uses quantitative methods. Where is the relationship between the independent variable and the dependent variable. The independent variable is pineapple filtrate and the dependent variable is vitamin C content. The parameters of this research are vitamin C levels using the UV Spectrophotometry method. The conclusion of this study shows that the results of the examination of pineapple juice levels are 54.6816 21.6678 mcg/g, and the vitamin C content in pineapple juice can meet the daily nutritional adequacy figure, namely 33 mg to 76 mg.
Produksi Protein Sel Tunggal dari Kultur Saccharomyces cerevisiae dengan Medium Limbah Kulit Nanas (Ananas comosus L. MERR) Krisna Juniharta Napitupulu; Yayuk Putri Rahayu; Haris Munandar Nasution; Minda Sari Lubis
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 2 (2024): March : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i2.346

Abstract

Single cell protein is the term used for peoteins derived from microbes such as fungi. Pineapple waste is one of the alternative media that has easiest source of carbohydrates to obtain, so it has the potential for the growth of Saccharomyces cerevisiae. The purpose of this study was to determine wether pineapple waste could produce single cell protein production by adding nutrients to the fermentation medium. The research used in this research is experimental research. To determine the relationship between the independent variable and the dependent variable. Where the independent variables are the addition of nutrients and fermentation time on days 0,2,4,6 to see the dependant variable, namely the analysis of protein content, dry weight analysis of cells, analysis of glucose levels, pH and temperature analysis. The results of this study showed that the highest protein content was obtained in H4 fermentation by MFKN2, namely 0,43%, cell dry weight 0,851 grams, glucose content 1,3402%, ph 4,5, temperature 27,1 ⁰C. Meanwile, MFKN1 showed the highest protein content in H6 fermentation, namely 0,38%, cell dry weight 0,817 grams, glucose content 1,3397%, pH 4, temperature 26,2 ⁰C. The yield of glucose and pH tended to decrease as the fermentation process progressed until the last day of fermentation. The results of the temperature analysis tend to be stable as the fermentation process progresses until the last day of fermentation. The conclusion of this study is that pineapple waste can produced single cell protein from Saccharomyces cerevisiae culture and has different protein production with the addition of nutrients in the fermentation medium.
Uji Intensitas Warna Kuning pada Campuran Sari Kunyit-Daun Salam dan Temulawak Daun Salam Sebangai Pewarna Pangan Putri Intan Sari; Anny Sartika Daulay; Ridwanto Ridwanto; Haris Munandar Nasution
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 3 (2024): May : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i3.363

Abstract

Some food industries still use synthetic dyes. Synthetic dyes, if consumed, can harm your health. Turmeric can be used as a natural food coloring. This natural coloring is applied to sweet jelly. This natural coloring agent must be used in fresh juice. To extend the use time of the natural dyes turmeric-bay leaves, ginger-bay leaves, this research was carried out in a variety of storage conditions without using preservatives. The aim of the research was to determine the results of the content of secondary metabolite compounds in turmeric-laurel extract, curcuma-laurel extract, turmeric extract and curcuma extract, in making solid preparations using preservatives, namely citric acid, maltodextin and sucrose. This research uses experimental methods with Sari method is done on a mixture of turmeric-bay leaves and ginger-bay leaves using a water solvent using the distillation method. Determination of variations in storage conditions was carried out for 7 days and the effect of storage temperature. Color stability and intensity measurements were carried out using visible spectrophotometry and characterization methods. The results of this research were obtained. The absorbance intensity test on turmeric-bay leaves was carried out at a wavelength of 425.14 nm with a result of 0.442 (day 1), ginger-bay leaves had an absorbance of 0.490 ( day 1). In the color stability test, there was a change in color from day 3 at drying cupboard temperature, at refrigerator temperature and at room temperature, the color remained stable.
Produksi Protein Sel Tunggal dari Aspergillus niger Kultur dengan Kulit Nanas (Ananas comosus L. MERR) Media Limbah Yuli Apriani Br.Lubis; Yayuk Putri Rahayu; Gabena Indrayani Dalimunthe; Haris Munandar Nasution
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 4 (2024): Juli : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i4.602

Abstract

Introduction: Single Cell Protein (SCP) is a term used for proteins produced from microbes such as bacteria, algae, yeasts, and fungi. One of the fungi known to produce PST is Aspergillus niger. Objective: The purpose of this study was to determine whether pineapple peel waste could produce single cell protein from A. niger culture and to determine differences in protein production with the addition of nutrients in the fermentation medium. Methods: This research method is an experimental method with the independent variables being MFKN1 medium (Pineapple Skin Fermentation Medium with the addition of KH2PO4 and sugar), and MFKN2 medium (Pineapple Skin Fermentation Medium with the addition of KH2PO4, (NH4)2SO4 and sugar); and the length of fermentation time used are days-0, 2, 4, and 6. The dependent variables of this study were analysis of protein content, dry weight of cells, glucose levels, pH and temperature. The data from this study were statistically analyzed using the two way Anova method. Results: The results of this study obtained the highest protein content in MFKN2 media, namely 0.80% (day-2); cell dry weight 0.473 g; glucose level 1.3406%; pH 4.6 and temperature 31 oC. Meanwhile, the highest protein content in MFKN1 media was 0.59% (day- 4); cell dry weight 0.346 g; glucose level 1.3406%; pH 3.7 and temperature 26.6 oC. Conclusion: The conclusion of this study is that pineapple peel waste can produce single cell protein from A. niger culture and there are differences in protein production results with the addition of nutrients, where protein content in MFKN2 medium is higher than protein content in MFKN1 medium.
UJI AKTIVITAS ANTIBAKTERI NANOPARTIKEL EKSTRAK ETANOL DAUN MANGGA HARUM MANIS (Mangifera indica L. var. arum manis) TERHADAP BAKTERI Escherichia Hutagaol, Delvi Amira; Rahayu, Yayuk Putri; Nasution, M. Pandapotan; Nasution, Haris Munandar
Jurnal Farmasi Klinik dan Sains Vol 3, No 1 (2023): Jurnal Farmasi Klinik dan Sains
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Gombong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jfks.v3i1.1109

Abstract

Bakteri Escherichia coli merupakan salah satu bakteri penyebab penyakit diare. Daun mangga harum manis (Mangifera indica L. var. harum manis) diketahui memiliki kandungan senyawa antibakteri. Dalam ukuran nanopartikel, luas kontak permukaan partikel menjadi lebih besar sehingga dapat meningkatkan aktivitas antibakteri. Tujuan penelitian ini untuk membandingkan aktivitas nanopartikel dengan ekstrak etanol daun mangga harum manis terhadap bakteri E. coli. Penelitian ini merupakan penelitian eksperimental dengan variabel bebas yaitu konsentrasi ekstrak etanol daun harum manis (EEDM 25%; EEDM 50%; dan EEDM 75%) dan konsentrasi nanopartikel ekstrak etanol daun mangga harum manis (NEDM 2,5%; NEDM 5%; dan NEDM 7,5%) dengan antibiotic pembanding yaitu tetracyclin.Variabel terikat yaitu aktivitas antibakteri ekstrak etanol dan nanopartikel ekstrak daun mangga harum manis terhadap bakteri E. coli. Karakterisasi nanopartikel ekstrak etanol menggunakan Particle Size Analyzer (PSA). Pengujian aktivitas antibakteri menggunakan metode difusi agar cakram Kirby Bauer. Hasil karakteristik ukuran nanopartikel ekstrak adalah 40,2 nm. Nilai Zone of Inhibition (ZOI) ekstrak etanol daun mangga harum manis adalah 12,4 mm (EEDM 25%); 14,1 mm (EEDM 50%); dan 16,1 mm (EEDM 75%). Nilai ZOI nanopartikel ekstrak etanol daun mangga harum manis adalah 13,8 mm (NEDM 2,5%); 14,5 mm (NEDM 5%); dan 18,6 mm (NEDM 7,5%). Kesimpulannya adalah ekstrak etanol daun mangga harum manis dapat dijadikan nanopartikel ekstrak dengan daya aktivitas antibakteri konsentrasi nanopartikel ekstrak 5% setara efektivitasnya dengan konsentrasi ekstrak etanol daun mangga harum manis 50% terhadap E. coli dengan kategori intermediate dibandingkan dengan Tetracyclin 30 μg, sehingga dapat dikatakan bahwa nanopartikel esktrak dapat memperkecil dosis suatu obat.
Drug Formulasi paper soap ekstrak etanol dedak padi (Oryza sativa L.) dan uji aktivitas antibakteri terhadap bakteri Escherichia coli dan Staphylococcus aureus Zannah , Muhamad; Dalimunthe , Gabena Indrayani; Lubis , Minda Sari; Yuniarti, Rafita
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.229

Abstract

Paper soap is soap in the form of thin sheets resembling paper. Rice bran from rice milling waste contains antibacterial compounds. The aim of this research was to formulate paper soap from rice bran ethanol extract and to determine its antibacterial activity. This research is a type of experimental research carried out in a laboratory. Includes steps such as taking and identifying samples, preparing and making ethanol extract of rice bran (Oryza sativa L.), and testing antibacterial activity against Escherichia coli using the Disc Disc method, where the inhibition zone of the ethanol extract of rice bran and the paper soap preparation is containing rice bran extract (Oryza sativa L.) was observed and measured as a result of the antibacterial activity test. The results of this research show that the ethanol extract of rice bran contains chemical compounds, namely alkaloids, flavonoids, saponins, tannins, triterpenoids/steroids and glycosides. Rice bran ethanol extract has characteristics in accordance with the 1989 MMI requirements. The results of determining the antibacterial activity of rice bran ethanol extract produce an inhibition zone against Escherechia coli bacteria ranging from 5mm - 12.5mm and Staphylococcus aureus ranging from 5.5 mm-16.67 mm. The results of the paper soap evaluation test, organoleptic examination, thickness examination, weight uniformity test, stability test, foam power test met the requirements and in the irritation tests F0, F1, F2 and F3 did not irritate the skin, in the hedonic test the most preferred formula was F1. The results of determining the antibacterial activity of paper soap produced an inhibition zone for Escherechia coli bacteria ranging from 8.16mm - 12.5mm and Staphylococcus aureus ranging from 15mm-14.83mm. From the research results it can be concluded that rice bran ethanol extract can be formulated into paper soap and has antibacterial activity.
Uji aktivitas antibakteri fraksi n-heksan dan etil asetat daun kenanga (Cananga odorata (Lam.) Hook. F. & Thomson) terhadap bakteri Staphylococcus epidermidis Sepriani, Ananda; Nasution , Haris Munandar; Mambang, D. Elysa Putri; Rahayu, Yayuk Putri
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.371

Abstract

Human life always coexists with various types of microbes. One of them is kenaga leaves (Cananga odorata (Lam.) Hook. F. & Thomson), which is a plant that can be used as a traditional medicine, one of which can be used to treat itching on human skin and can inhibit bacteria, one of which is Staphylococcus epidermidis. This research aimed to determine the types of secondary metabolite compounds contained in ylang-ylang leaves (Cananga odorata (Lam.) Hook. F. & Thomson) and to test the antibacterial activity of the n-hexane and ethyl acetate fractions of ylang-ylang leaves (Cananga odorata (Lam.) Hook. F. & Thomson). This research method includes simplicia characterization and phytochemical screening on simplicia powder and 96% ethanol extract. Extraction was done by maceration with 96% ethanol solvent, then liquid-liquid fractionation with n-hexane and ethyl acetate solvents. The antibacterial activity test was carried out by measuring the diameter of the inhibition zone using the agar diffusion method. The results of the examination of the characteristics of ylang-ylang leaf Simplicia powder (Cananga odorata (Lam.) Hook. F. & Thomson) showed that the water content was 7.33%, the water-soluble essence content was 26.48%, the total ash was 3.52%, and acid insoluble ash content 0.18%. The results of phytochemical screening showed alkaloids, flavonoids, saponins, glycosides, tannins, and steroids. The activity test results on the n-hexane, ethyl acetate, and ethanol extract fractions had antibacterial activity. The n-hexane fraction gave strong results against Staphylococcus epidermidis bacteria at a 70 mg/ml concentration with an inhibitory zone diameter of 12.37 mm. The ethyl acetate fraction was strong against Staphylococcus epidermidis bacteria at a 70 mg/ml concentration with an inhibition zone diameter of 12.98 mm.
Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol daun mangga kasturi (Mangifera casturi Koesterm.) dari Gampong Drien Bungong, Pidie Jaya dengan metode DPPH Mulyani, Fitri; Rahayu, Yayuk Putri; Daulay, Anny Sartika; Nasution, Haris Munandar
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.374

Abstract

Antioxidants are chemical compounds that, in specific amounts, can inhibit or slow down cell damage caused by free radicals. Antioxidants function to protect the body from free radical attacks. The more free radicals in the body, the more cells will be damaged. One plant that contains antioxidant compounds is casturi mango (Mangifera casturi Koesterm.). Casturi mango (Mangifera casturi Koesterm.) is a plant that contains secondary metabolite compounds such as saponins, tannins, triterpenoids, flavonoids, and phenolics which have potential as antioxidants. This research aimed to determine differences in secondary metabolite compounds and antioxidant activity of ethanol extract of casturi mango leaves (Mangifera casturi Koesterm.) growing in Gampong Drien Bungong, Pidie Jaya. The stages of this research include sample processing, making ethanol extract of kasturi mango leaves, characterization examination, phytochemical screening, and antioxidant activity test. The sample used was musk mango leaves. The ethanol extract of casturi mango leaves (Mangifera casturi Koesterm.) was extracted using maceration with 96% ethanol solvent. The antioxidant activity test was carried out using the DPPH (1-1-Diphenyl-2-Picrylhydrazyl) method using a UV-Vis spectrophotometer at a maximum wavelength of 516 nm and vitamin C as a comparison. Based on the results of phytochemical screening tests, there are differences in secondary metabolites in the ethanol extract of kasturi mango leaves in Gampong Drien Bungong, Pidie Jaya, namely alkaloids, flavonoids, tannins, saponins, steroids, and glycosides, and the results of antioxidant activity obtained an IC50 value of 9.06 ppm with the category very strong, equivalent to the antioxidant potential of vitamin C, having an IC50 value of 4.63 ppm in the robust category.  
Co-Authors Adelia Ramadani Adjelie, Tiara Adyani Maulinda Ahmad Rasyid Ridho Ainil Fithri Pulungan Alaina, Nadila Alfi Wahyudi Nasution Amaliana, Amaliana Ananda , Nadia Andjelie, Tiara Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Apmarja, Sarah Utami Aris Munandar Aryunda, Divia Asmarani, Asmarani Aulia Fitri, Aulia Aulia Sari Auza , Maharani Ayu , Tri Indah Pagar Azhar, Gustika Barutu, Liza Anisa Shevia Basaniah Basaniah Bilal, Mohd. BJ Sutrisna Cici Andriani Cindy Priscilia Cindy Widya Rosa Br Simarmata Cut Dian Mala Luthfia Cut Erika Maulydya D Elysa Putri Mambang D Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang Daeng Elysa Putri Mambang Daulay , Anny Sartika Delvi Amira Hutagaol Dikki Miswanda Dina Agustia Parlin Dina Suciati Saragih Dinda Sari Utami Dini Rahmadani Dwi Jati, Fatma Ellyza, Chella Emmy Wiriandini Ercha Minanda Fahira, Nelsa Fahmi Fahmi Fathur Rahman Fatimah, Cut fatur Rahman, fatur Febiyani, Rica Fithri Pulungan, Ainil Fitri Mulyani, Fitri Fitri, Nurul Fitri, Raissa Friyani, Lilah Gabena Indrayani Dalimunthe Hafizha, Putri Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Supia Indah Hastri Kholifah Humaira, Yara Hutagalung , Septi Ratna Cempaka Hutagalung, Riris Br Hutagaol, Delvi Amira Huzeila Nisa Siregar Ihsan Fadhilah Ika Yeni Siahaan Ilmi Widya Sari Imelda Natasia Br S Meliala Indah Nasution Indah Triutami Harahap Indrayani, Gabena Junia Fitri Hayani Khofifah , Nur Kiki Rawitri Krisna Juniharta Napitupulu Leni Safriani Lestari, Sri Indah Linda, Rosa Liza Zulaini Lubis, Minda Sari lubis, muhammad fauzan Lubis, Nadya Frestika Luthfianida, Arifna M Pandapotan Nasution M Pandapotan Nasution M Pandapotan Nasution M. Alfaruqi M. Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution Mambang , D.Elysa Putri Mambang, D. Elsya Putri Mambang, Elysa Putri Maya Syafira Mayani, Nurhikma Meutia, Cut Miftahul Jannah Minda Sar Lubis Muhammad Amin Nasution Muhammad Ari Mukhtizar Muhammad Hizbullah Muhammad Pandapotan Nasution Muhammad Wahyu Ikhsan, Muhammad Wahyu Ikhsan Munaziatul Jannah Munthe, Herlina Nasri Nasri Nasri Nasri, Nasri Nasution , Muhammad Amin Nasution, Kartika Zsaskia Nasution, M Pandapotan Nasution, M. Pandapotan Nasution, Nur Sahadah Nasution, Wardah Natasya, Siti Adinda Nazirah, Nazirah Nazuhra, Oca Nelsa Fahira Ningtias, Anggitha Niswa, Khairun Niza Fonna Nofa Lismandaria Novika Utami Nur Hanifah Nuraida Nuraida Nuratika, Eli Nurliansyah, Nurliansyah Nursyafira, Arini Nurul Hasanah Ovalina Sylvia Br. Ginting Pandapotan Nasution, M. Pertiwi, Rahma Prasetyo , Healthy Aldriany Purba , Intan Sabila Putri Puteri, Cut Intan Annisa Putri , Adilla Putri Intan Sari Putri Khairani Putri Mambang, D. Elysa Putri Rahayu, Yayuk Putri, Nadya Iwani Rafika, Intan Maya Rafita Yuniari Rafita Yuniart Rafliza , Rista Rahayu , Yayuk Putri Rahmadani , Adevika Rapita Yuniarti Rati , Yuliandini Rawitri, Kiki Ridho, Ahmad Rasyid Ridwanto Ridwanto Ridwanto Rifqi, M. Ainur Rina Azhari Ritonga , Septi Ani Riza Syafira Rizki, Ayu Fadillah RR. Ella Evrita Hestiandari Rukmana, Siti Sabila , Anisa Safira , Liyuza Safitri , Nurhaliza sahrianti, Hikmah Sari, Syilvi Rinda Sarmadansyah Sarmadansyah Sartika Daulay, Anny Sasnita, Merida Satri Situmorang, Rati Selfia Lestary Sepriani, Ananda Sinaga, Novita Yulianti Siregar , Anisah Siregar, Huzeila Nisa Siti Aisyah Jamil Situmorang, Lusi Grasia Situmorang, Rati Satri Sony , Suyefri Sri Harti Sri Natalia Saragih Sri Ria Ranti Sri Wahyuni Sri Wahyuni Sri Wahyuni Suci, Nurul Sulwiyatul K Sani Supiyani, Supiyani Syafira, Nona Syaiful Bahri Syalsabila Putri Syara, Nurdiani Syarifa Mayly Syarifah Elena Gultom Tawir Rina Rezeki Tri Damaiyanti Trizaldi, Asep Try Santi Kisria Darsih Ummi Khairani Rambe Vera Estefania Kaban Vera Estefania Kaban Wahdaniati , Tri Widya Sari Widya Sari Widya, Rizka Yani, Farida Yanti, Rahma Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yuli Apriani Br.Lubis Yuniarti , Rafita Yuniarti, Rafita Zannah , Muhamad Zikra Maqfirah Ziza Putri Aisyia Fauzi Zulmai Rani