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Formulasi sediaan pasta daging lidah buaya (Aloe vera (L.)Burm.f.) dan uji aktivitas antibakteri terhadap bakteri Streptococcus mutans Meutia, Cut; Dalimunthe , Gabena Indrayani; Lubis , Minda Sari; Yuniarti , Rafita
Journal of Pharmaceutical and Sciences JPS Volume 7 Nomor 4 (2024)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v7i4.524

Abstract

Introduction: Aloe vera (Aloe vera (L.) Burm.f.) is a plant that contains 95% water. The rest is in the form of other active ingredients (essential oils, amino acids, minerals, vitamins, enzymes, and glycoproteins). The type of aloe vera used to make herbal aloe vera is the Barbadensis type, which contains substances for human needs, such as Vitamins A, B1, B2, B6, B12, Vitamin E, and Vitamin C. This plant can also cure diabetes, heart disease, and other diseases.  Aloe vera can be formulated into toothpaste preparations because it can improve hygiene and reduce plaque in the mouth. Objective: This study aimed to make a new formulation of (aloe vera (L.) Burm.f.) as a toothpaste preparation and evaluate its antibacterial activity against Streptococcus mutans bacteria. Methods: This study uses an experimental method. In this study, aloe vera was formulated as toothpaste. The physical evaluation carried out included tests for water content, total ash content, water-soluble extract content, ethanol-soluble extract content, acid-insoluble extract content, organoleptic test, oh test, homogeneity test, spreadability test, adhesion test, test viscosity, and foam formation test. Results: Based on the research results, it can be seen that aloe vera flesh has antibacterial activity against Streptococcus mutans bacteria. Conclusion: This research concludes to make a new formulation of (aloe vera (L.) Burm.f.) as a toothpaste preparation and evaluate its antibacterial activity against Streptococcus mutans bacteria.
Penentuan kadar flavonoid total ekstrak etanol kayu kuning (Arcangelisia flava (L.) Merr. ) dan uji antibakteri Staphylococcus aureus . Nuraida, Nuraida; Ridwanto, Ridwanto; Daulay , Anny Sartika; Nasution , Haris Munandar
Journal of Pharmaceutical and Sciences JPS Volume 7 Nomor 3 (2024)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v7i3.598

Abstract

Indonesia has a diversity of plant species used as traditional medicine, one of which is yellow wood (Arcangelisia flava (L.) Merr.), this yellow wood is antibacterial, anti-inflammatory, antiseptic and antipyretic. It is known that yellow wood contains saponins, flavonoids, alkaloids, tannins, and steroids that can treat malaria, fever, rheumatism and itching. This study aims to determine the value of total flavonoid content contained in yellow wood extract in various ethanol concentrations (50%, 70% and 96%) and to determine the antibacterial inhibition zone in yellow wood extract. The stages of this study include processing plant materials, making ethanol extracts of yellow wood with ethanol concentrations of 50%, 70% and 96% using the maceration method then the extract obtained is concentrated with a rotary evaporator, examining characteristics, phytochemical screening, determining the total flavonoid content of yellow wood extract concentrations (50%, 70% and 96%) by UV-Vis spectrophotometric method and antibacterial test of Staphylococcus aureus  by diffusion method. The results showed that ethanol extract of yellow wood contains alkaloid, flavonoid, tannin, saponin, and steroid/terpenoid compounds. The results of the determination of total flavonoid content in yellow wood extract at 50% concentration amounted to 0.815200888 ± 0.02445546 mg QE/g, 70% concentration amounted to 1.0660462967 ± 0.03265799 mg QE/g and at 96% concentration amounted to 1.6781149 ± 0.0085 mg QE/g. Antibacterial test on 96% yellow wood extract obtained an inhibition zone of 18.05 mm with a strong category.
Penentuan konsentrasi hambat minimum dan konsentrasi bunuh minimum ekstrak dan nanopartikel ekstrak daun pepaya (Carica Papaya L.) terhadap Bakteri Cutibacterium acnes Khofifah , Nur; Rahayu, Yayuk Putri; Nasution , Haris Munandar; Miswanda, Dikki
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.720

Abstract

One of the skin diseases that often occurs is acne (Acne vulgaris). Acne is caused by the activity of the Cutibacterium acnes bacteria. Current acne treatment still depends on the use of antibiotics, but long-term use of antibiotics can cause bacterial resistance. Therefore, it is necessary to develop alternative treatments for acne that are effective and safe, one of which is by using medicinal plants. One plant that has potential is papaya leaves (Carica papaya L.). This research aims to determine the minimum inhibitory concentration (MIC) and minimum killing concentration (KBM) of ethanol extract and papaya leaf extract nanoparticles as well as testing antibacterial activity against Cutibacterium acnes bacteria. The research was carried out experimentally. The independent variables are the concentration of papaya leaf extract (6.25% concentration, 12.5% ​​concentration, 25% concentration and 50% concentration), and the concentration of papaya leaf extract nanoparticles (0.625% concentration, 1.25% concentration, 2.5% concentration % and Concentration 5%). The dependent variable is the antibacterial activity of papaya leaf ethanol extract and nanoparticles extract against Cutibacterium acnes. Characterization of the size of the extracted nanoparticles using a Particle Size Analyzer (PSA). The characteristic results for the extract size were 2,203.45 nm while the size of the extract nanoparticles was 330.27 nm. The minimum inhibitory concentration (MIC) value of 1.25% papaya leaf ethanol extract nanoparticles is better than 12.5% ​​papaya leaf ethanol extract, and the minimum lethal concentration (KBM) value of 5% papaya leaf ethanol extract nanoparticles is better than papaya leaf ethanol extract 50% against Cutibacterium acnes bacteria. 5% papaya leaf ethanol extract nanoparticles have the same antibacterial ability as 50% papaya leaf ethanol extract and are sensitive to Cutibacterium acnes bacteria, so it can be said that 5% papaya leaf ethanol extract nanoparticles can reduce the dose concentration of antibacterial compounds up to one-tenth of the time compared to ethanol extract papaya leaves 50% (1:10)
Penetapan kadar flavonoid ekstrak metanol kulit kayu raru (Cotylelobium lanceolatum Craib) pada variasi konsentrasi pelarut menggunakan metode spektrofotometri UV-Vis Nasution, Kartika Zsaskia; Daulay, Anny Sartika; Ridwanto, Ridwanto; Nasution, Haris Munandar
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.724

Abstract

The use of plant-based traditional medicine remains a common practice in developing countries, with more than 80% of the population relying on it for healthcare and treatment. In Indonesia, natural medicines continue to be a primary choice for public health, aligning with local traditions and cultural practices. One such medicinal plant is raru bark, which possesses various pharmacological activities, including antidiabetic, astringent, antilaxative, antibacterial, antiseptic, antidiarrheal, and blood-clotting properties. This study aims to characterize the methanol extract of raru bark and determine its flavonoid content using UV-Vis spectrophotometry. The research stages included plant material processing, methanol extraction, characterization tests, macroscopic and microscopic examinations, phytochemical screening, and flavonoid quantification. Phytochemical analysis revealed that the methanol extract of raru bark contains flavonoids, tannins, saponins, and triterpenoids. Characterization tests showed that all evaluated parameters met the required standards. Flavonoid quantification was performed by determining the maximum wavelength of quercetin using UV-Vis spectrophotometry at different solvent concentrations. The results indicated that the total flavonoid content at a 96% solvent concentration was 1.625 ± 0.153 mgQE/g, at 70% was 1.318 ± 0.003 mgQE/g, and at 50% was 1.146 ± 0.006 mgQE/g.
Penetapan kadar flavonoid total ekstrak etanol 70% dan ekstrak etil asetat daun sambung nyawa (Gynura procumbens (Lour)Merr.) secara spektrofotometri UV-Vis Rahmadani , Adevika; Ridwanto, Ridwanto; Daulay , Anny Sartika; Nasution , Haris Munandar
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.751

Abstract

Sambung nyawa leaves (Gynura procumbens L.) are a simplicia with various medicinal properties. One of the chemical compounds in sambung nyawa leaves that plays a crucial role in treatment is flavonoids. Flavonoids in sambung nyawa leaves have benefits as antihypertensive, antioxidant, antihyperglycemic, and anti-inflammatory agents. Different drying methods for simplicia affect the total flavonoid content in the extract.The stages of this research included plant material processing, the preparation of ethanol and ethyl acetate extracts, characterization tests, phytochemical screening, and the determination of total flavonoid content in the ethanol extract and ethyl acetate extract of sambung nyawa leaves using visible spectrophotometry. The extracts of sambung nyawa leaves were made using the maceration method with 70% ethanol and ethyl acetate, and the obtained extracts were concentrated using a rotary evaporator. Total flavonoid content was then determined using visible spectrophotometry. Phytochemical screening of the ethanol and ethyl acetate extracts of sambung nyawa leaves revealed the presence of chemical compounds such as alkaloids, flavonoids, saponins, tannins, and steroids/triterpenoids. The total flavonoid content in the ethanol extract was found to be 6.5149 ± 0.0314 mcg/g of sample, while the ethyl acetate extract had a total flavonoid content of 77.6695 ± 0.1199 mcg/g of sample.
Formulasi orally disintegrating tablet dari serbuk kembang kol (Brassica oleracea var. botrytis L.) dengan variasi konsentrasi sari tapai sebagai disintegrant Lestari, Sri Indah; Dalimunthe, Gabena Indrayani; Lubis, Minda Sari; Nasution, Haris Munandar
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.773

Abstract

Cauliflower (Brassica oleracea var. botrytis L.) has a nutritional content consisting of calcium, magnesium, potassium, phosphorus, and low saturated fat. It has vitamin C, which helps increase body immunity. Orally Disintegrating Tablet preparations can dissolve quickly when placed on the tongue, formulated for ease of use of drugs for patients who have difficulty swallowing. Therefore, disintegrants are very important in ODT preparations. Tapai extract has a sweet, slightly sour taste and disintegrates immediately when placed on the tongue. This research aimed to determine whether cauliflower powder with tapai extract disintegrants can be formulated into ODT preparations and which formulation is the best.  This research included characterization of simplicia, making pre-formulation by mixing all ingredients, evaluating pre-formulation, and tablet printing by direct compression method. Then, the tablet evaluation was carried out. The disintegrant was tapai extract with 5%, 7.5%, and 10% concentrations. Data were analyzed using the One Way ANOVA method. The results of the formulation evaluation, the flow time test obtained <10 seconds, which is easy to flow; the angle of repose test obtained 26˚, the category is very good; and the compressibility test obtained 18-20%, which is quite good. The results of the tablet evaluation in the organoleptic test obtained round tablets, sweet taste, brownish-white color, and distinctive odor. The weight uniformity test on all formulas did not have any deviating weights; the size uniformity test met the requirements, the hardness test obtained 1-3 kg, the friability test obtained 0.4-0.7%, the petri dish disintegration time test obtained 44.07-52.48 seconds, and the mouth disintegration time test obtained 53.07-57.78 seconds and the wetting time test obtained 53.21-54.87 seconds. The best formula is formula 3, with a 10% tapai extract concentration.
Konsentrasi hambat minimum dan konsentrasi bunuh minimum ekstrak etanol dan nanopartikel ekstrak etanol daun matoa (Pometia pinnata) terhadap Cutibacterium acnes Safira , Liyuza; Rahayu, Yayuk Putri; Nasution , Haris Munandar; Miswanda, Dikki
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.786

Abstract

Infectious diseases are caused by the entry and proliferation of microorganisms, including bacteria, fungi, parasites, and viruses. These diseases occur when microbial interactions lead to host tissue damage, resulting in various clinical symptoms and signs. This study aimed to formulate nanoparticles of ethanol extract from matoa leaves and compare the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, as well as evaluate the antibacterial activity of both the extract and its nanoparticles against Cutibacterium acnes. This research was conducted experimentally. The independent variables were the concentrations of matoa leaf extract (6.25%, 12.5%, 25%, and 50%) and matoa leaf extract nanoparticles (0.625%, 1.25%, 2.5%, and 5%). The dependent variable was the antibacterial activity of both formulations against Escherichia coli and Cutibacterium acnes. Nanoparticle size characterization was performed using a Particle Size Analyzer (PSA), yielding an average size of 528.95 nm. The MIC values for C. acnes were 12.5% for the extract and 1.25% for the nanoparticles, while the MBC values were 50% and 5%, respectively. The highest antibacterial activity of matoa leaf ethanol extract against C. acnes was observed at a concentration of 50% (inhibition zone: 27.86 mm), whereas the nanoparticles exhibited a maximum inhibition zone of 26.53 mm at a 5% concentration. These findings indicate that matoa leaf extract nanoparticles exhibit strong antibacterial activity against C. acnes at lower concentrations compared to the crude extract, suggesting their potential as an effective antibacterial agent. 
Penetapan kadar flavonoid total dan aktivitas antioksidan ekstrak etanol, fraksi n-heksan, etil asetat daun senggani (Melastoma candidum D.Don) secara spektrofotometri visibel Apmarja, Sarah Utami; Nasution, Muhammad Amin; Nasution , Haris Munandar; Yuniarti , Rafita
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.797

Abstract

Medicinal plants are natural sources that have antioxidant activity, characterized by the content of phenolic and flavonoid components in reducing free radicals which depends on the number of hydroxyl groups in their molecular structure. One of the plants that contain flavonoid compounds which have antioxidant activity is senggani leaves (Melastoma candidum D.Don). This research aimed to determine the chemical compounds contained in the ethanol extract, to determine the total flavonoid value of the ethanol extract, the ethyl acetate fraction and the n-hexane fraction and to determine the antioxidant activity of the ethanol extract, the ethyl acetate fraction and n-hexane fraction. The stages of this research include processing plant materials, making ethanol extract, making ethyl acetate fraction and n-hexane fraction, characterization examination, phytochemical screening, and determining the total flavonoid content of ethanol extract, ethyl acetate fraction and n-hexane fraction of senggani leaves using Visible and Spectrophotometric methods. Determination of the antioxidant activity of ethanol extract, ethyl acetate fraction, and n-hexane fraction using different techniques, namely DPPH with ABTS. The results of phytochemical screening on simplicia powder and ethanol extract contained chemical compound groups such as alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids, and glycosides. The results of determining the total flavonoid content in the ethanol extract of senggani leaves were 44.7805 ± 0.176606439 mgQE/g; In the ethyl acetate fraction, it was 48.421 ± 0 mgQE/g, and in the n-hexane fraction it was 31.491 ± 0.091844121 mgQE/g. The results of antioxidant activity using the DPPH method obtained an IC₅₀ value for the ethanol extract of 12.51 (μg/ml); in the ethyl acetate fraction of 9.42 (μg/ml); in the n-hexane fraction, it was 40.45 (μg/ml), and in the vitamin C standard it was 4.32 (μg/ml). Antioxidant results using the ABTS method obtained an IC₅₀ value for the ethanol extract of 8.21(μg/ml); in the ethyl acetate fraction of 5.93(μg/ml); in the n-hexane fraction, it was 23.35(μg/ml), and in the vitamin C odor it was 2.65(μg/ml).
Uji toksisitas menggunakan metode BSLT dan uji antibakteri Staphylococcus aureus dan Escherichia coli ekstrak dan fraksi batang karamunting (Rhodomyrtus tomentosa (Aiton) Hassk.) Siregar , Anisah; Ridwanto, Ridwanto; Daulay , Anny Sartika; Nasution , Haris Munandar
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.822

Abstract

Natural ingredients have been widely used in medicine, commonly referred to as "back to nature," meaning "kembali ke alam." Herbal medicine is recognized as one of the most practical and effective treatment modalities available. One medicinal plant is the stem of Rhodomyrtus tomentosa (Aiton) Hassk., which belongs to the Myrtaceae family and has the potential as an antibacterial remedy. To ensure traditional medicine fulfills its responsibility, scientific research, such as toxicity testing, is necessary. The Brine Shrimp Lethality Test (BSLT) is a preliminary test to assess the toxic effects of plants. This study aims to determine the secondary metabolite compounds, toxicity levels, and antibacterial activity of ethanol extract and n-hexane and ethyl acetate fractions of Rhodomyrtus tomentosa stem. The study includes characterization, phytochemical screening, and toxicity testing by observing the mortality rate of Artemia salina Leach larvae, expressed as LC50. Antibacterial testing against Staphylococcus aureus and Escherichia coli was conducted using the disc diffusion method. The results of phytochemical screening of simplicia powder, ethanol extract, and n-hexane and ethyl acetate fractions of Rhodomyrtus tomentosa stem tested positive for alkaloids and triterpenoids/steroids. Simplicia powder, ethanol extract, and the ethyl acetate fraction also contained secondary metabolites such as flavonoids, tannins, and saponins. The toxicity test results using probit analysis showed that the LC50 value of the extract was 97.6787 µg/mL, the LC50 value of the ethyl acetate fraction was 71.4331 µg/mL, and the LC50 value of the n-hexane fraction was 57.4910 µg/mL. Therefore, it can be concluded that the extract and the n-hexane and ethyl acetate fractions of Rhodomyrtus tomentosa stem are toxic. The antibacterial test results showed that the extract and the n-hexane and ethyl acetate fractions exhibited inhibitory activity against Staphylococcus aureus and Escherichia coli.
Aktivitas Antibakteri Ekstrak Etanol Daun Ruku-Ruku (Ocimum tenuiflorum L.) Terhadap Bakteri Staphylococcus epidermidis Alaina, Nadila; Mambang, Elysa Putri; Nasution, M. Pandapotan; Nasution, Haris Munandar
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 2 (2023): September 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i2.8107

Abstract

Daun ruku-ruku (Ocimum tenuiflorum L.) merupakan salah satu tanaman obat yang dikenal masyarakat digunakan sebagai bumbu masakan karena aromanya yang dapat mengurangi bau tidak sedap. Berdasarkan kandungan kimianya, daun ruku-ruku banyak mengandung alkaloid, flavonoid, tanin, saponin dan steroid/triterpenoid. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak etanol daun ruku-ruku terhadap bakteri Staphylococcus epidermidis. Metode penelitian dilakukan secara eksperimental dengan variabel bebas yaitu ekstrak etanol daun ruku-ruku dengan konsentrasi 5%, 10% dan 20% sedangkan variabel terikatnya adalah aktivitas antibakteri terhadap Staphylococcus epidermidis. Ekstrak sederhana daun ruku-ruku dibuat dengan pelarut etanol 96%. Kontrol positif yang digunakan adalah Tetrasiklin dan kontrol negatif adalah DMSO. Pengujian yang dilakukan terhadap simplisia daun ruku-ruku meliputi pemeriksaan fitokimia, pemeriksaan makroskopis, pemeriksaan mikroskopis, pemeriksaan kadar air, pemeriksaan nira larut air, pemeriksaan kadar sari larut etanol, pemeriksaan kadar abu dan pemeriksaan kadar abu tidak larut asam. Hasil skrining fitokimia menunjukkan bahwa daun ruku-ruku (Ocimum tenuiflorum L.) mengandung senyawa metabolit sekunder yaitu alkaloid, flavonoid, tanin, saponin dan streroid/triterpenoid. Hasil penelitian aktivitas antibakteri menunjukkan bahwa daun ruku-ruku dapat digunakan sebagai antibakteri karena mempunyai daya hambat yang kuat pada konsentrasi 5%, 10% dan 20% yaitu 8,7 mm, 9,8 mm dan 12,1 mm terhadap bakteri Staphylococcus epidermidis
Co-Authors Adelia Ramadani Adjelie, Tiara Adyani Maulinda Ahmad Rasyid Ridho Alaina, Nadila Alfi Wahyudi Nasution Amaliana, Amaliana Ananda , Nadia Andjelie, Tiara Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Apmarja, Sarah Utami Aris Munandar Aryunda, Divia Asmarani, Asmarani Aulia Fitri, Aulia Aulia Sari Auza , Maharani Ayu , Tri Indah Pagar Azhar, Gustika Barutu, Liza Anisa Shevia Basaniah Basaniah Bilal, Mohd. BJ Sutrisna Cici Andriani Cindy Priscilia Cindy Widya Rosa Br Simarmata Cut Dian Mala Luthfia Cut Erika Maulydya D Elysa Putri Mambang D Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang Daeng Elysa Putri Mambang Daulay , Anny Sartika Delvi Amira Hutagaol Dikki Miswanda Dina Agustia Parlin Dina Suciati Saragih Dinda Sari Utami Dini Rahmadani Dwi Jati, Fatma Ellyza, Chella Emmy Wiriandini Ercha Minanda Fahmi Fahmi Farida Yani Fathur Rahman Fatimah, Cut fatur Rahman, fatur Febiyani, Rica Fithri Pulungan, Ainil Fitri Mulyani, Fitri Fitri, Nurul Fitri, Raissa Friyani, Lilah Gabena Indrayani Dalimunthe Gultom, Syarifah Elena Hafizha, Putri Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Supia Indah Hastri Kholifah Humaira, Yara Hutagalung , Septi Ratna Cempaka Hutagalung, Riris Br Hutagaol, Delvi Amira Huzeila Nisa Siregar Ihsan Fadhilah Ika Yeni Siahaan Ilmi Widya Sari Imelda Natasia Br S Meliala Indah Nasution Indah Triutami Harahap Indrayani, Gabena Junia Fitri Hayani Khofifah , Nur Kiki Rawitri Krisna Juniharta Napitupulu Leni Safriani Lestari, Sri Indah Linda, Rosa Lubis, Minda Sari lubis, muhammad fauzan Lubis, Nadya Frestika Luthfianida, Arifna M Pandapotan Nasution M Pandapotan Nasution M Pandapotan Nasution M. Alfaruqi M. Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution Mambang , D.Elysa Putri Mambang, D. Elsya Putri Mambang, Elysa Putri Maya Syafira Mayani, Nurhikma Meutia, Cut Miftahul Jannah Minda Sar Lubis Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Ari Mukhtizar Muhammad Hizbullah Muhammad Pandapotan Nasution Muhammad Wahyu Ikhsan, Muhammad Wahyu Ikhsan Munaziatul Jannah Munthe, Herlina Nasri Nasri, Nasri Nasution , Muhammad Amin Nasution, Kartika Zsaskia Nasution, M. Pandapotan Nasution, Nur Sahadah Nasution, Wardah Natasya, Siti Adinda Nazirah, Nazirah Nazuhra, Oca Nelsa Fahira Ningtias, Anggitha Niswa, Khairun Nofa Lismandaria Novika Utami Nur Hanifah Nuraida Nuraida Nuratika, Eli Nurliansyah, Nurliansyah Nursyafira, Arini Nurul Hasanah Ovalina Sylvia Br. Ginting Pandapotan Nasution, M. Pertiwi, Rahma Prasetyo , Healthy Aldriany Purba , Intan Sabila Putri Puteri, Cut Intan Annisa Putri , Adilla Putri Intan Sari Putri Khairani Putri Mambang, D. Elysa Putri Rahayu, Yayuk Putri, Nadya Iwani Rafika, Intan Maya Rafita Yuniari Rafita Yuniart Rafliza , Rista Rahayu , Yayuk Putri Rahmadani , Adevika Rapita Yuniarti Rati , Yuliandini Rawitri, Kiki Ridho, Ahmad Rasyid Ridwanto Ridwanto Ridwanto Ridwanto Ridwanto Rifqi, M. Ainur Rina Azhari Ritonga , Septi Ani Riza Syafira Rizki, Ayu Fadillah RR. Ella Evrita Hestiandari Rukmana, Siti Sabila , Anisa Safira , Liyuza Safitri , Nurhaliza sahrianti, Hikmah Sari, Syilvi Rinda Sarmadansyah Sarmadansyah Sartika Daulay, Anny Sasnita, Merida Satri Situmorang, Rati Selfia Lestary Sepriani, Ananda Sinaga, Novita Yulianti Siregar , Anisah Siti Aisyah Jamil Situmorang, Lusi Grasia Situmorang, Rati Satri Sony , Suyefri Sri Harti Sri Natalia Saragih Sri Ria Ranti Sri Wahyuni Sri Wahyuni Sri Wahyuni Suci, Nurul Sulwiyatul K Sani Supiyani, Supiyani Syafira, Nona Syaiful Bahri Syalsabila Putri Syara, Nurdiani Syarifah Elena Gultom Tawir Rina Rezeki Tri Damaiyanti Trizaldi, Asep Try Santi Kisria Darsih Ummi Khairani Rambe Vera Estefania Kaban Wahdaniati , Tri Widya Sari Widya Sari Widya, Rizka Yanti, Rahma Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yuli Apriani Br.Lubis Yuniarti , Rafita Yuniarti, Rafita Zannah , Muhamad Zikra Maqfirah Ziza Putri Aisyia Fauzi Zulmai Rani