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Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis Hanifah, Nur; Daulay, Anny Sartika; Rahman, Fatur; Nasution, Haris Munandar
Journal of Pharmaceutical and Health Research Vol 5 No 1 (2024): February 2024
Publisher : Forum Kerjasama Pendidikan Tinggi (FKPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jharma.v5i1.4788

Abstract

The Bajakah tampala tree (Spatholobus littoral hassk) is a native plant from the interior of the forests in Kalimantan, especially Central Kalimantan. This plant is a plant that stretches or grows and grows abundantly in the interior of the forest. The Bajakah plant is known to the people of Kalimantan as a medicinal plant and can cure various diseases, including diarrhea, rheumatism, and cancer. The aim of this research was to determine the alkaloid content of bajakah root extract in various concentrations. The research method was carried out experimentally, with the independent variable being an ethanol extract of bajakah roots with concentrations of 50, 70, and 96%. Meanwhile, the dependent variables are the characteristics of simplicia, the secondary metabolite content of Bajakah root ethanol extract, and the determination of the total alkaloid content of Bajakah root extract. Bajakah root simplicia extract is made with 96, 70, and 50% ethanol solvents. The parameters used in this study include water content, total ash content, acid insoluble ash content, water soluble extract content, ethanol soluble extract content, and secondary metabolite content, including alkaloids, tannins, flavonoids, saponins, steroids, and terpenoids, by conducting phytochemical screening. , making a standard caffeine solution, determining the maximum wavelength of caffeine, measuring the caffeine calibration curve, and determining the total alkaloid content of Bajakah root extract. The results of the phytochemical screening showed that Bajakah root was positive for containing alkaloid compounds, saponins, tannins, flavonoids, steroids, and terpenoids. And the results obtained from Bajakah root plants obtained from Central Kalimantan were a 96% ethanol concentration of 1.66797 ± 0.7088 mg/gram, a 70% ethanol concentration of 1.6679 ± 0.1124 mg/gram, and a 50% ethanol concentration of 0.46981 ± 1.0656 mg/gram
ANTIBACTERIAL TEST OF ETHANOLIC EXTRACT NANOPARTICLES FROM ARUM MANIS MANGO LEAVES (MANGIFERA INDICA L. VAR. ARUM MANIS) AGAINST STAPHYLOCOCCUS AUREUS Lubis, Nadya Frestika; Rahayu, Yayuk Putri; Nasution, Haris Munandar; Lubis, Minda Sari
Jurnal FARMASIMED (JFM) Vol 5 No 2 (2023): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v5i2.1687

Abstract

Secondary metabolites with antibacterial properties have been found in arummanis mango leaves. Alkaloids, flavonoids, tannins, saponins, triterpenoids, and steroids, especially the mangiferin molecule found in several mango varieties, are also present in arum manis mango leaf extract. Mangiferin has shown action on Staphylococcus aureus as an antibacterial agent that can prevent the development of bacteria. Staphylococcus aureus is one of the bacteria that triggers skin infections. In this research, extract nanoparticles and inhibition tests against staphylococcus aureus bacteria were prepared using extract nanoparticles and ethanol extract of arum manis mango leaves. The manufacture of nanoparticles in this study was carried out because smaller and nanometer-sized materials have more special chemical and glass characteristics; their small size has a greater surface area to volume ratio than larger particles. Nanoparticles are small particles ranging in size from 1 to 1000 nm. A particle size analyzer is used to characterize nanoparticles. Using the disc diffusion method, antibacterial activity tests were carried out at concentrations of 25%, 50%, and 75%, using nanoparticle extracts, and 2.5%, 5%, and 7.5% for extract concentration. The results of the extract nanoparticle size characterization were 40.2 nm. The bacterial inhibition zones of arum manis mango leaf extract obtained at concentrations of 25%, 50%, and 75% were 11.9, 14.4, and 15.4 mm, respectively. Meanwhile, the inhibition zones of nanoparticle bacteria extract 2.5%, 5%, and 7.5% were 15.5, 17.5, and 18.3 mm, respectively. The concentration of 2.5% nanoparticle extract has the inhibition power of bacteria equivalent to that of 75% ethanol extract, so it says that the nanoparticles can reduce the size dose and have an inhibition power in the moderate sensitivity category.
PENETAPAN KADAR FENOLIK TOTAL EKSTRAK METANOL KAYU KUNING (Arcangelisia flava (L.) Merr.) DENGAN BERBAGAI KONSENTRASI METANOL DETERMINATION OF TOTAL PHENOLIC CONTENT OF YELLOW WOOD (Arcangelisia flava (L.) Merr.) Methanol EXTRACT WITH VARIOUS METHANOL Hasanah, Qori; Daulay, Anny Sartika; Ridwanto, Ridwanto; Nasution, Haris Munandar
Medic Nutricia : Jurnal Ilmu Kesehatan Vol. 8 No. 3 (2024): Medic Nutricia : Jurnal Ilmu Kesehatan
Publisher : Cahaya Ilmu Bangsa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5455/nutricia.v8i3.6085

Abstract

Salah satu tanaman yang mengandung senyawa metabolit sekunder yaitu kayu kuning (Arcangelisia flava (L.) Merr.) yang terkandung dalam batangnya adalah alkaloid, fenolik, flavonoid saponin, dan tanin. Senyawa fenolik mempunyai aktivitas sebagai antioksidan. Senyawa fenolik adalah senyawa kimia yang memiliki ikatan rangkap terkonjugasi dan gugus kromofor dapat ditentukan kadarnya menggunakan metode spektrofotometri. Perbedaan dari metode dan penggunaan pelarut untuk menyari ekstrak berbeda dapat mempengaruhi kadar dan jenis senyawa fenolik yang akan diperoleh. Tujuan dari penelitian ini adalah untuk mengetahui kandungan metabolit sekunder dan kandungan total kadar fenolik dari ekstrak metanol kayu kuning dengan berbagai konsentrasi dari pelarut methanol 99,8%, 70% dan 50% menggunakan Spektrofotometri UV–VIS. Hasil skrining fitokimia pada ekstrak metanol kayu kuning bahwa terdapat kandungan golongan senyawa kimia seperti alkaloid, flavonoid, fenolik, tanin, steroid/triterpenoid, saponi dan glikosida. Penentuan kadar fenolik total dilakukan dengan menentukan panjang gelombang maksimum asam galat, operating time, pengukuran kurva kalibrasi asam galat dan perhitungan kadar fenolik total dengan menggunakan metode spektrofotometri UV-Vis. Hasil penentuan kadar fenolik total pada ekstrak metanol kayu kuning 99,8% sebesar 81,61002 ± 0,248610176 mg GAE/g sampel, 70% sebesar 68,8756 ± 0,24837736 mg GAE/g sampel dan 50% sebesar 57,7894 ± 0,13110763709 mg GAE/g sampel. One of the plants that contains secondary metabolite compounds is yellow wood (Arcangelisia flava (L.) Merr.), which contains alkaloids, phenolics, flavonoids, saponins and tannins in its stems. Phenolic compounds have activity as antioxidants. Phenolic compounds are chemical compounds that have conjugated double bonds and chromophore groups that can be determined using spectrophotometric methods. Differences in methods and use of solvents to extract different extracts can influence the levels and types of phenolic compounds that will be obtained. The aim of this research was to determine the secondary metabolite content and total phenolic content of yellow wood methanol extract with various concentrations of 99.8%, 70% and 50% methanol solvent using UV-VIS Spectrophotometry. The research results Phytochemical screening of methanol extract of yellow wood showed that it contained chemical compounds such as alkaloids, flavonoids, phenolics, tannins, steroids/triterpenoids, saponi and glycosides. Determination of total phenolic content was carried out by determining the maximum wavelength of gallic acid, operating time, measuring the gallic acid calibration curve and calculating total phenolic content using the UV-Vis spectrophotometric method. The results of determining the total phenolic content in 99.8% methanol extract of yellow wood were 81.61002 ± 0.248610176 mg GAE/g, 70% were 68.8756 ± 0.24837736 mg GAE/g and 50% were 57.7894 ± 0 .13110763709 mg GAE/g sample).
KARAKTERISASI SIMPLISIA SKRINING FITOKIMIA DAN UJI TOKSISITAS EKSTRAK ETANOL HERBA BINTARO (Cerbera manghas L.) TERHADAP Artemia salina Leach Nasution, Haris Munandar; Fatimah, Cut; Syara, Nurdiani
Jurnal Farmanesia Vol 6 No 1 (2019): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v6i1.2741

Abstract

The use of anticancer chemotherapy with chemicals has not given optimal results because the drug does not work specifically, in addition to attacking cancer cells it also damages normal cells. Bintaro (Cerbera mangha L.) is a wild plant that contains secondary metabolites which have been shown to have a wide range of therapeutic applications. Several studies have stated that Bintaro has activities as aanti cancer. The purpose of this researchto know the characteristics of Bintaro herb simplicia (Cerbera manghas L.) which can be determined using the procedures listed in the Materia Medika Indonesia book, knowing the class of secondary metabolites contained in the ethanol extract of bintaro herbs (Cerbera manghas L.), andknowing the level of toxicity of the ethanol extract of the herb bintaro (Cerbera manghas L.). The method used in this research is experimental with the post-test only controlled group design. The results of the characterization on the organoleptic test of Bintaro herb simplicia, namely the leaves are dark brown, the taste is slightly bitter then bitter, the stems are dark brown and shrinking, the smell is distinctive and tasteless, and the flowers are dark brown, characteristic odor and tasteless, after being ground to a powder. dark brown. The results of the microscopic test, the leaf herb simplicia samples obtained fragments, namely diasitic type stomata, spiral vessel fragments and upper epidermis fragments with palisade cells. As for the stem herbs, there were tissue fragments of sclerenchyma fibers, vessel fragments, parenchymal fragments with clumps of brown secretions. Sclereid fragments were found in the floral herb samples.6%, for determination of water-soluble extract content obtained = 20.06%, ethanol soluble extract content = 2.18%, total ash content = 12.62%, acid insoluble ash content = 6.11%. positive bintaro herbs contain compounds of alkaloids, flavonoids, steroids, tannins and saponins. From the calculation of the LC50 value, it can be concluded that the entanol extract of the Bintaro herb is included in the very toxic category with a value ofLC50 < 30 g/mlthat is9.35g/mL.
SKRINING FITOKIMIA DAN PENETAPAN KADAR FLAVONOID TOTAL TERHADAP EKSTRAK ETANOL SAWI PAHIT (Brassica juncea (L.) Czern) DENGAN METODE SPEKTROFOTOMETRI UV-VIS Mambang, D. Elsya Putri; Nasution, Haris Munandar; Friyani, Lilah
Jurnal Farmanesia Vol 8 No 2 (2021): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v8i2.3421

Abstract

Mustard is a vegetable commodity that has good commercial value and prospects. Apart from being viewed from the climatological, technical and social economic point of view, it is also very supportive, so that it has the feasibility to be cultivated in Indonesia and this vegetable is a type of vegetable that is favored by all groups of people. Mustard is a vegetable that has many benefits for the body.The stages of this research include sample collection and plant processing, plant identification, manufacture of ethanol extract of bitter mustard leaf, determination of water content, phytochemical screening and determination of total flavonoid content by UV-Visible spectrophotometry.This study aims to determine the total flavonoid content of bitter mustard greens and to determine the characteristics, class of chemical compounds, and total flavonoid value of bitter mustard (Brassica juncea (L.) Czern) extract by UV-VIS spectrophotometry.
ANALISIS BIOAUTOGRAFI DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP BAKTERI Propionibacterium acne Nasution, Haris Munandar; Situmorang, Rati Satri
Jurnal Farmanesia Vol 9 No 1 (2022): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v9i1.3424

Abstract

Noni or (Morinda citrifolia L.) contains active anthraquinone, scaloptin, flavonoid, and adaptogen compounds that have antifungal and antimicrobial effects. Antibacterial chemicals have many negative side effects, and other alternatives are needed as antibacterials, one of which is by using noni. This study aims to analyze bioautography and determine the antibacterial activity of noni leaf ethanol extract against Propionibacterium acne bacteria.
Produksi Protein Sel Tunggal dari Kultur Bacillus cereus dengan Medium Limbah Cair Tahu Basaniah Basaniah; Yayuk Putri Rahayu; Haris Munandar Nasution; Gabena Indrayani Dalimunthe
Vitalitas Medis : Jurnal Kesehatan dan Kedokteran Vol. 1 No. 2 (2024): April : Vitalitas Medis : Jurnal Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/vimed.v1i2.152

Abstract

Single Cell Protein (PST) is the term used for proteins derived from microbes such as fungi, yeasts and bacteria. One way to deal with the abundance of tofu liquid waste is by using microbiological methods, namely utilizing tofu liquid waste as a substrate for the growth of Bacillus cereus microbial growth for single cell protein production. The purpose of this study was to determine whether tofu liquid waste could produce single cell protein from B. cereus culture and to determine the difference in protein production by adding nutrients to the fermentation medium. The research method used was experimental research, with the independent variables being MFLT1 fermentation medium (Tofu Liquid Waste Fermentation Medium with the addition of KH2PO4 nutrients and sugar) and MFLT2 medium (Tofu Liquid Waste Fermentation Medium with the addition of nutrients KH2PO4; (NH4)2SO4 and sugar); and the length of fermentation on days 0, 2, 4 and 6. While the dependent variables were the analysis of protein content, cell dry weight, glucose content, pH and temperature. The result data in this study were statistically analyzed using the two way Anova method. The results of this study showed that the highest protein content was obtained in MFLT2 medium, namely 0.73% (day 4); cell dry weight 0.253 g; glucose level 1.3339%; pH 3.9 and temperature 29.2oC. Meanwhile, in MFLT1 medium, the highest protein content was 0.46% (4th day); cell dry weight 0.286 g; glucose level 1.3342%; pH 3.9 and temperature 28.8oC. From the research that has been carried out, it can be concluded that tofu liquid waste can produce single cell protein from B. cereus culture and there are differences in protein production results with the addition of nutrients, where the protein content in MFLT2 medium is higher than protein content in MFLT1 medium.
UJI AKTIVITAS ANTIBAKTERI DAUN SENGGANI (Melastoma candidum D.Don) DENGAN VARIASI EKSTRAKSI MASERASI DAN MICROWAVE ASSISTED EXTRACTION TERHADAP S. aureus DAN E. coli Hasibuan, Supia Indah; Nasution, Muhammad Amin; Ridwanto; Nasution, Haris Munandar
Jurnal Kesehatan Tambusai Vol. 5 No. 3 (2024): SEPTEMBER 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v5i3.33460

Abstract

Penyakit infeksi merupakan salah satu permasalahan kesehatan yang sering terjadi.  Jenis infeksi bisa dipicu oleh bakteri gram postif maupun gram negatif. Salah satu mikroorganisme yang dapat menyebabkan infeksi yaitu Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan mengidentifikasi senyawa metabolit sekunder dalam ekstrak etanol daun Senggani (Melastoma candidum D.Don) dan mengevaluasi aktivitas antibakterinya terhadap Staphylococcus aureus dan Escherichia coli. Jenis penelitian yang dilakuakan adalah penelitian eksperimental. Sampel tumbuhan yang digunakan dalam penelitian ini adalah daun senggani (Melastoma candidum D.Don) yang diperoleh dari Kabupaten Tapanuli Tengah. Metode pengambilan sampel dengan metode purposive yaitu tanpa membandingkan tumbuhan serupa didaderah lain. Data yang diperoleh dengan pengukuran diameter zona hambat ekstrak etanol daun senggani (Melastoma candidum D.Don) dengan metode ekstraksi maserasi dan microwave assisted extraction dari masing-masing konsentrasi dengan 3 kali pengulangan. Data yang diperoleh dianalsis dengan menggunakan uji Oneway Annova. Parameter yang digunakan dalam penelitian ini adalah uji skrining fitokimia, metode ekstraksi maserasi dan microwave assisted extraction dan uji antibakteri. Hasil penelitian menunjukkan bahwa ekstrak daun Senggani yang diperoleh melalui metode maserasi mengandung alkaloid, flavonoid, saponin, tanin, dan steroid. Ekstrak dari metode MAE juga mengandung senyawa-senyawa tersebut, serta glikosida tambahan. Aktivitas antibakteri ekstrak daun Senggani terhadap Staphylococcus aureus dan Escherichia coli menunjukkan bahwa ekstrak dari metode MAE memberikan zona hambat yang lebih besar dibandingkan dengan ekstrak dari metode maserasi, menunjukkan efektivitas yang lebih tinggi dalam aktivitas antibakteri.
Aktivitas Antibakteri Ekstrak Etanol Daun Ruku-Ruku (Ocimum tenuiflorum L.) Terhadap Bakteri Staphylococcus epidermidis Alaina, Nadila; Mambang, Elysa Putri; Nasution, M. Pandapotan; Nasution, Haris Munandar
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 2 (2023): September 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i2.8107

Abstract

Daun ruku-ruku (Ocimum tenuiflorum L.) merupakan salah satu tanaman obat yang dikenal masyarakat digunakan sebagai bumbu masakan karena aromanya yang dapat mengurangi bau tidak sedap. Berdasarkan kandungan kimianya, daun ruku-ruku banyak mengandung alkaloid, flavonoid, tanin, saponin dan steroid/triterpenoid. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak etanol daun ruku-ruku terhadap bakteri Staphylococcus epidermidis. Metode penelitian dilakukan secara eksperimental dengan variabel bebas yaitu ekstrak etanol daun ruku-ruku dengan konsentrasi 5%, 10% dan 20% sedangkan variabel terikatnya adalah aktivitas antibakteri terhadap Staphylococcus epidermidis. Ekstrak sederhana daun ruku-ruku dibuat dengan pelarut etanol 96%. Kontrol positif yang digunakan adalah Tetrasiklin dan kontrol negatif adalah DMSO. Pengujian yang dilakukan terhadap simplisia daun ruku-ruku meliputi pemeriksaan fitokimia, pemeriksaan makroskopis, pemeriksaan mikroskopis, pemeriksaan kadar air, pemeriksaan nira larut air, pemeriksaan kadar sari larut etanol, pemeriksaan kadar abu dan pemeriksaan kadar abu tidak larut asam. Hasil skrining fitokimia menunjukkan bahwa daun ruku-ruku (Ocimum tenuiflorum L.) mengandung senyawa metabolit sekunder yaitu alkaloid, flavonoid, tanin, saponin dan streroid/triterpenoid. Hasil penelitian aktivitas antibakteri menunjukkan bahwa daun ruku-ruku dapat digunakan sebagai antibakteri karena mempunyai daya hambat yang kuat pada konsentrasi 5%, 10% dan 20% yaitu 8,7 mm, 9,8 mm dan 12,1 mm terhadap bakteri Staphylococcus epidermidis
SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MATOA (Pometia pinnata J.R. FORST & G. FORST) TERHADAP PERTUMBUHAN BAKTERI Salmonella thypi Gultom, Syarifah Elena; Mambang, D. Elysa Putri; Nasution, Haris Munandar; Rahayu, Yayuk Putri
Jurnal Farmasi Klinik dan Sains Vol 3, No 1 (2023): Jurnal Farmasi Klinik dan Sains
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Gombong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jfks.v3i1.1062

Abstract

Matoa memiliki sejarah panjang dalam penggunaan obat tradisional (Pometia pinnata J.R. Forst & G. Forst). Antioksidan termasuk vitamin C dan E, serta flavonoid, tanin, dan saponin dapat ditemukan dalam jumlah yang melimpah pada buah matoa. Penelitian ini bertujuan untuk mengetahui sifat antibakteri dari ekstrak etanol serbuk simplisia daun matoa dan metabolit sekundernya terhadap Salmonella thypi. Variabel bebas dalam penelitian ini adalah ekstrak etanol daun matoa (Pometia pinnata J.R. Forst & G. Forst). Pengujian dilakukan pada ekstrak 50%, 30%, dan 30%. Aktivitas antibakteri ekstrak etanol daun matoa ditentukan dari kemampuannya dalam menghambat pertumbuhan Salmonella typhi. Pelarut etanol digunakan untuk menguapkan ekstrak kental yang diperoleh dari hasil maserasi daun matoa. Ekstrak daun matoa diencerkan dengan DMSO 30%, 40%, dan 50%. Pengenceran tersebut akan menentukan seberapa baik ketahanan Salmonella typhi terhadap antibiotik. Metabolit sekunder seperti flavonoid, tanin, alkaloid, saponin, dan steroid dianalisis pada ekstrak etanol daun matoa dan simplisia. Salmonella thypi dihambat dengan rata-rata 12,6 mm pada konsentrasi 30% (Interpretasi menengah), 13,8 mm pada konsentrasi 40%, dan 15,3 mm pada konsentrasi 50% dengan menggunakan ekstrak etanol daun matoa. Aktivitas antibakteri dari ekstrak etanol daun matoa ditunjukkan dengan zona hambat dengan diameter rata-rata daerah hambat yang sesuai dengan interpretasi Intermediate (I). 
Co-Authors Adelia Ramadani Adjelie, Tiara Adyani Maulinda Ahmad Rasyid Ridho Alaina, Nadila Alfi Wahyudi Nasution Amaliana, Amaliana Ananda , Nadia Andjelie, Tiara Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Apmarja, Sarah Utami Aris Munandar Aryunda, Divia Asep Trizaldi Asmarani, Asmarani Aulia Fitri, Aulia Aulia Sari Auza , Maharani Ayu , Tri Indah Pagar Azhar, Gustika Barutu, Liza Anisa Shevia Basaniah Basaniah BJ Sutrisna Cici Andriani Cindy Priscilia Cindy Widya Rosa Br Simarmata Cut Dian Mala Luthfia Cut Erika Maulydya D Elysa Putri Mambang D Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang Daeng Elysa Putri Mambang Daulay , Anny Sartika Delvi Amira Hutagaol Dikki Miswanda Dina Agustia Parlin Dina Suciati Saragih Dinda Sari Utami Dini Rahmadani Ellyza, Chella Emmy Wiriandini Ercha Minanda Farida Yani Fathur Rahman Fatimah, Cut Fatma Dwi Jati fatur Rahman, fatur Febiyani, Rica Fithri Pulungan, Ainil Fitri Mulyani, Fitri Fitri, Nurul Fitri, Raissa Friyani, Lilah Gabena Indrayani Dalimunthe Gultom, Syarifah Elena Hafizha, Putri Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Supia Indah Hastri Kholifah Hutagalung , Septi Ratna Cempaka Hutagalung, Riris Br Hutagaol, Delvi Amira Huzeila Nisa Siregar Ihsan Fadhilah Ika Yeni Siahaan Ilmi Widya Sari Imelda Natasia Br S Meliala Indah Nasution Indah Triutami Harahap Indrayani, Gabena Junia Fitri Hayani Khofifah , Nur Kiki Rawitri Krisna Juniharta Napitupulu Leni Safriani Lestari, Sri Indah Linda, Rosa Lubis, Minda Sari Lubis, Nadya Frestika Luthfianida, Arifna M Pandapotan Nasution M Pandapotan Nasution M Pandapotan Nasution M. Alfaruqi M. Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution Mambang , D.Elysa Putri Mambang, D. Elsya Putri Mambang, Elysa Putri Maya Syafira Meutia, Cut Miftahul Jannah Minda Sar Lubis Mohd. Bilal Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Ari Mukhtizar Muhammad Hizbullah Muhammad Pandapotan Nasution Muhammad Wahyu Ikhsan Muhammad Wahyu Ikhsan Munaziatul Jannah Munthe, Herlina Nasution , Muhammad Amin Nasution, Kartika Zsaskia Nasution, M. Pandapotan Nasution, Nur Sahadah Natasya, Siti Adinda Nazirah, Nazirah Nazuhra, Oca Nelsa Fahira Ningtias, Anggitha Niswa, Khairun Nofa Lismandaria Novika Utami Nur Hanifah Nuraida Nuraida Nuratika, Eli Nurliansyah, Nurliansyah Nurul Hasanah Ovalina Sylvia Br. Ginting Pertiwi, Rahma Prasetyo , Healthy Aldriany Purba , Intan Sabila Putri Puteri, Cut Intan Annisa Putri , Adilla Putri Intan Sari Putri Khairani Putri Mambang, D. Elysa Putri Rahayu, Yayuk Putri, Nadya Iwani Rafika, Intan Maya Rafita Yuniari Rafita Yuniart Rafita Yuniarti Rafliza , Rista Rahayu , Yayuk Putri Rahmadani , Adevika Rapita Yuniarti Rati , Yuliandini Rati Satri Situmorang Rawitri, Kiki Ridho, Ahmad Rasyid Ridwanto Ridwanto Ridwanto Ridwanto Ridwanto Rina Azhari Ritonga , Septi Ani Riza Syafira RR. Ella Evrita Hestiandari Rukmana, Siti Sabila , Anisa Safira , Liyuza Safitri , Nurhaliza sahrianti, Hikmah Sari, Syilvi Rinda Sarmadansyah Sarmadansyah Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Sepriani, Ananda Sinaga, Novita Yulianti Siregar , Anisah Siti Aisyah Jamil Situmorang, Lusi Grasia Situmorang, Rati Satri Sony , Suyefri Sri Harti Sri Natalia Saragih Sri Ria Ranti Sri Wahyuni Sri Wahyuni Sri Wahyuni Sulwiyatul K Sani Supiyani, Supiyani Syafira, Nona Syalsabila Putri Syara, Nurdiani Syarifah Elena Gultom Tawir Rina Rezeki Tri Damaiyanti Ummi Khairani Rambe Wahdaniati , Tri Widya Sari Widya Sari Widya, Rizka Yanti, Rahma Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yuli Apriani Br.Lubis Yuniarti , Rafita Yuniarti, Rafita Zannah , Muhamad Zikra Maqfirah Ziza Putri Aisyia Fauzi Zulmai Rani