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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Aquaculture and Fish Health Serambi Journal of Agricultural Technology Buletin Jalanidhitah Sarva Jivitam Emasains : Jurnal Edukasi Matematika dan Sains Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Biodiversitas Journal of Biological Diversity

MUHAMMAD AGUS SUPRAYUDI

Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University, Bogor, West Java. Indonesia

Author-ID : 393767

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Library & Information Science Mathematics Mechanical Engineering Medicine & Pharmacology Veterinary

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Development of Siganid (Siganus guttatus) larvae during the transition period Darsiani Darsiani; Mia Setiawati; Dedi Jusadi; Muhammad Agus Suprayudi; Asda Laining
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Siganid is better known as rabbit fish. In hatcheries, constraint that is still faced is the low survival, which was assumed to occur because of the timing for initial feeding is not solidly known. This research aimed to examine the best initial feeding time for siganus, based on evaluation on eyes and yolk reserves during the transition. The research was conducted from 24-29 March 2021 in IPUW Barru, South Sulawesi. Larvae were obtained from the second progeny (G2) of domesticated Siganus guttatus. Larvae were reared for 5-6 days without feeding. Evaluated parameters include eyes diameter and yolk reserves. Samples were observed with microscope and will be explained descriptively. Water quality parameters were measured, namely DO, salinity, pH and temperature. Eyes diameter at 6 Hour After Hatching (HAH) ranges between 81.5-128.9 µm, 13 HAH= 125.5-167.7 µm, 24 HAH= 138.2-213.9 µm, two days after hatching 2 Day After Hatching (DAH) = 113.6-193.1 µm, 3 DAH= 163.1-219.2 µm, 4 DAH= 190.4-212.6 µm. Yolk reserves diameter ranged between 137-194µm (6 HAH), 13 HAH= 152-191µm, 24 HAH= 94.0-185µm, 2 DAH= 75.3-99.63µm, 3 DAH= 42.33-87.58µm, 4 DAH= 38.17-55.59µm. After age 5 DAH, there are no larvae found alive (dead). Eyes developed at age 6 HAH and experienced pigmentation at age 24 HAH. Conversely, yolk reserves diameters were getting smaller since age 24 HAH and completely disappear at age 4 DAH. It indicates that eyes effectively see feeds at age 2 DAH. Therefore, initial feeding should be started. The water quality parameters measured were still in normal conditions according to the life of S. guttatus larvae. From this research, it can be concluded that eyes have been well functioned at age 2 DAH and yolk reserves was finished at age 4 DAH. Therefore, the initial feeding should be done at the age of 2 DAH.Keywords:Development, Siganus guttatus larvae,Transition

Administration of Microencapsulated Probiotic at Different Doses to Control Streptococcosis in Tilapia (Oreochromis niloticus) DIAH AYU SATYARI UTAMI; WIDANARNI - WIDANARNI; M AGUS SUPRAYUDI
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

This study aimed to produce microencapsulated probiotic and determine its optimal dose to control streptococcosis in tilapia (Oreochromis niloticus). The probiotic used in this study was Bacillus sp. NP5 RfR that was encapsulated by sterile 10% maltodextrin solution and dried by spray dryer. The experimental fish were reared 28 days and fed by the administration of microencapsulated probiotic in feed with different doses (0,5% (A), 1% (B), and 2% (C)) which were 1010 CFU g-1 as the concentration and control without administration of microencapsulated probiotic, including negative (K-) and positive (K+) control. On day 30, all of the fish except K- were challenged by injecting 0.1 mL/fish Â of Streptococcus agalactiae (105 CFU ml-1) by intra-peritoneal (IP) route. This study showed that administration of 0.5% microencapsulated probiotic was effective to control streptococcosis in tilapia with higher post-challenge survival rate, better hematological parameter values, and could inhibit S. agalactiae growth in the host target organs.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22296

Siganid is better known as rabbit fish. In hatcheries, constraint that is still faced is the low survival, which was assumed to occur because of the timing for initial feeding is not solidly known. This research aimed to examine the best initial feeding time for siganus, based on evaluation on eyes and yolk reserves during the transition. The research was conducted from 24-29 March 2021 in IPUW Barru, South Sulawesi. Larvae were obtained from the second progeny (G2) of domesticated Siganus guttatus. Larvae were reared for 5-6 days without feeding. Evaluated parameters include eyes diameter and yolk reserves. Samples were observed with microscope and will be explained descriptively. Water quality parameters were measured, namely DO, salinity, pH and temperature. Eyes diameter at 6 Hour After Hatching (HAH) ranges between 81.5-128.9 m, 13 HAH= 125.5-167.7 m, 24 HAH= 138.2-213.9 m, two days after hatching 2 Day After Hatching (DAH) = 113.6-193.1 m, 3 DAH= 163.1-219.2 m, 4 DAH= 190.4-212.6 m. Yolk reserves diameter ranged between 137-194m (6 HAH), 13 HAH= 152-191m, 24 HAH= 94.0-185m, 2 DAH= 75.3-99.63m, 3 DAH= 42.33-87.58m, 4 DAH= 38.17-55.59m. After age 5 DAH, there are no larvae found alive (dead). Eyes developed at age 6 HAH and experienced pigmentation at age 24 HAH. Conversely, yolk reserves diameters were getting smaller since age 24 HAH and completely disappear at age 4 DAH. It indicates that eyes effectively see feeds at age 2 DAH. Therefore, initial feeding should be started. The water quality parameters measured were still in normal conditions according to the life of S. guttatus larvae. From this research, it can be concluded that eyes have been well functioned at age 2 DAH and yolk reserves was finished at age 4 DAH. Therefore, the initial feeding should be done at the age of 2 DAH.Keywords:Development, Siganus guttatus larvae,Transition

Effect of feedings with different protein levels and dietary supplemental rElGH on culture performances of sex reversed Oreochromis niloticus (Linnaeus, 1758) Muhammad Safir; alimuddin alimuddin; Mia Setiawati; muhammad Agus Suprayudi; Muhammad Zairin Junior
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22550

This study aims to evaluate the culture performance of tilapia (Oreochromis niloticus) that have been treated with 17-methyltestosterone (MT) and without MT (w-MT), feed with different protein levels (20, 24, and 28%) and recombinant Epinephelus lanceolatus growth hormone (rElGH)-diet. The research was conducted in 9 treatments and triplicate. Tilapia larvae were soaked twice, totaling 500 larvae, at the age of 10 days after hatching (DAH) and the age of 14 DAH was soaked for 4 hours using 1/l MT 2 mg/l solution. Fish maintenance was conducted in an aquarium of 1.0x0.5x0.5 m3 in the first month, and three months later in net cages (2.0x2.0x1.5 m3). Daily growth rate (DGR) and biomass gain (BG) were increased in line with increasing feed protein content and rElGH supplementation. The highest DGR and BG values were MT+28+rElGH (P 0.05) treatment. The highest feed consumption and the lowest feed conversion ratio were also obtained in the MT+28+rElGH treatment (P 0.05). Fish survival was ranged from 79.89 to 90.28% (P 0.05). The highest profit potential was found in the MT+28+rElGH treatment. The efficient aquaculture can be obtained by feeding sex-reversed tilapia at a protein level of 28% and a diet supplemented with rElGH.Keywords:Feed conversion ratioGrowth hormoneProtein retention17-methyltestosterone

Immersion Effect of Estradiol-17β on Cannibalism of Asian Redtail Catfish (Hemibagrus nemurus) Post Larvae Benny Heltonika; Agus Oman Sudrajat; Muhammad Junior Zairin; Widanarni Widanarni; Muhammad Agus Suprayudi; Wasmen Manalu; Yani Hadiroseyani
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.38173

Highlight Research The dose, duration of immersion, and time interval of immersion of the estradiol-17β affect the rate of cannibalism and increase the seed survival of Asian redtail catfish in low treatment in this research. Over optimum treatment increased the incidence of cannibalism at Asian redtail catfish and decrease growth performance. Treatment of estradiol-17β, give the ability of Asian redtail catfish to live on high density The treatment of estradiol-17β, has an effect on concentration plasma of body testosterone and cortisol Abstract The cannibalistic behavior of Asian redtail catfish greatly hampers the supply of these fish seeds, invoking the need to be controlled. Estradiol is one of the hormones that has been known to reduce cannibalistic behavior on fish. This study aimed to evaluate the effect of estradiol-17β immersion on the incidence of cannibalism in the rearing of post larvae of Asian redtail catfish. Post larvae was treated with a combination of doses of estradiol hormone 0, 1, and 2 ppm and immersion time of two and four hours with immersion intervals of three and six days with a completely randomized design. Each treatment had a fish density of 10 fish L-1, with mean size individual length of 6.73 ± 0.73 mm (three days of age after hatched), with four replications. Fish were fed with tubifex up until satiation point and reared for 30 days. The results showed that the administration of the estradiol-17β through immersion with a dose of 1 ppm for two hours and interval of six days was able to reduce the level of cannibalism and increase the survivability. A further increase in dose, immersion time, and interval had the opposite effect. Estradiol-17β immersion with certain dose, duration, and interval affected the rate of cannibalism, growth rate, and survival. Estradiol-17β immersion at a dose of 1 ppm for two hours and six days interval was the best to reduce the level of cannibalism and normal mortality in post larvae of Asian redtail catfish.

Feminization of Maroon Clownfish (Amphiprion biaculeatus, Bloch 1790) with 17β-Estradiol Hormonal Induction Helena Sahusilawane; Agus Oman Sudrajat; Muhmmad Agus Suprayudi; Dinar Tri Soelistyowati; Ligaya I. T. A. Tumbelaka; Irzal Effendi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.42765

Highlight Research The 17β-estradiol hormone induction reduces the percentage of R values in skin and fin color by as much as 50-60%. The 17β-estradiol hormone induction increases total length and body weight, estradiol content, GIS and HIS, and also glucose content. The 17β-estradiol hormone induction shows mature development of the gonadal profile from functional male to functional female. Abstract As maroon clownfish (Amphiprion biaculeatus) is a protandrous hermaphroditic fish, feminization process with 17β-estradiol hormone can be applied to accelerate the female broodstock candidate supply for further spawning effort. This study aimed to evaluate the feminization of A. biaculeatus with 17β-estradiol hormonal induction. This study used a completely randomized design with several hormone dosage, namely P0 (without 17β-estradiol hormone induction), P1 (0.5 Î¼g 17β-estradiol/g body), P2 (1.0 Î¼g 17β-estradiol/g body), and P3 (1.5 Î¼g 17β-estradiol/g body). These treatments were applied with three replications. Five fish composed of α-fish, β-fish, and three Î³-fish were reared in each aquarium for 90 days with a flowing water system. The α- and β-fish were then removed, while the Î³-fish was injected with hormone. Otohime pellet feed was fed three times a day until apparent satiation. The results showed that the 17β-estradiol hormone could induce 100% of the feminization process of male A. biaculeatus. The dosage of P3 obtained the lowest value percentage of red, green, blue (RGB), but showing the highest total of length and body weight (6.67±0.42 cm and 6.40±0.78 g, respectively), estradiol content (149.73±4.24 Ïg/mL), GSI and HSI (0.38±0.07% and 3.59±0.49%), and glucose content (4.67±0.64 mg/dL), followed by more mature gonad profile than other treatments. This condition indicates that fish in P3 treatment has been reversed as functional female. The average survival rate for the treatment was as high as 60%. Therefore, the application of 17β-estradiol hormonal induction is effective for the feminization process in A. biaculeatus as a protandrous hermaphroditic fish.

CANNIBALISM PERFORMANCE OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) FED RATION SUPPLEMENTED WITH DIFFERENT DOSES OF 17α-METHYLTESTOSTERONE Heltonika, Benny; Sudrajat, Agus Oman; Junior, Muhammad Zairin; Widanarni, Widanarni; Suprayudi, Muhammad Agus; Manalu, Wasmen; Hadiroseyani, Yani
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.27-35

One of the obstacles in Asian redtail catfish hatchery is the high cannibalism incidence. Cannibalism is associated with aggressive behavior caused by hormonal metabolism especially of testosterone. The purpose of this study was to evaluate the effect of testosterone administration on the cannibalism incidence in the Asian redtail catfish juveniles. The experiment was conducted by rearing fish with initial length of 4.09±0.19 cm in 16 of 20 L aquariums with a density of 2 fish L-1. The experiment was designed with a completely randomized design with 4 treatments which were different level of 17α-methyltestosterone supplementation in feed, i.e., 0 mg kg-1 feed (A) as control, 7.5 mg kg-1 feed (B), 15 mg kg-1 feed (C), and 30 mg kg-1 feed (D). Each treatment has 4 replications. Fish fed experimental diet (40% protein) 4 times a day to satiation for 30 days. The parameters observed were type and index of cannibalism, aggressive behavior, survival rate, normal mortality, growth performance, hormones concentrations (estradiol, testosterone, and cortisol), and water quality. The results showed that cannibalism type II (the fish eaten completely or missing) and cannibalism index increased with the increasing doses of testosterone administration in the feed with the highest cannibalism incidence was 40.63%. The highest survival rate was found in treatment B (73.75±2.50%) and was not significantly different from the control treatment (69.38±2.39%). No differences in testosterone concentration and in the growth performance among the treatments. However, there was a trend of decrease in the estradiol concentration of Asian redtail catfish juveniles fed ration supplemented with the increasing doses of 17α-methyltestosterone. Based on the results obtained in this research, estradiol changed in the body's plasma, it appears that there was a role for plasma estradiol concentration in controlling cannibalism of Asian redtail catfish juveniles. It concluded that the testosterone administration affected the cannibalism incidence in the Asian redtail Catfish.

Effectiveness of NSP enzyme and organic chromium supplementation in low-protein feed on carbohydrate utilization in Pomfret Colossoma macropomum Faiqotul Himmah, Maihardiyanti; Achmad Fauzi, Ichsan; Ekasari, Julie; Raditya Gumelar, Muhammad; Muhammad Agus Suprayudi
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.21-31

This study aims to evaluate the effectiveness of NSP enzyme and organic chromium supplementation in low-protein Colosoma macropomum diet to increase carbohydrate utilization. The diets contains isoprotein (20%) and isoenergy. Factorial design with two factors, namely NSP enzyme (0 and 1 g/kg) and organic chromium (0, 1, and 2 mg/kg). Fish are reared in an aquarium measuring 80 x 50 x 35 cm3 and containing 75 L of water and stock at the density of 15 fish and reared for 60 days. The test parameters used are postprandial blood glucose levels, antioxidant activity, glycogen in muscle and liver, and growth performance. The results showed that the highest postprandial blood glucose levels were when the NSP enzyme was administered. Furthermore, the lowest malondialdehyde (MDA) values and the highest superoxide dismutase (SOD) and glutathione peroxide (GPx) values were obtained in fish fed feed containing 1 g/kg of NSP enzyme and 1 mg/kg organic chromium. Fish fed feed containing 1 g/kg NSP enzyme and 1 mg/kg organic chromium showed significant values in liver and muscle glycogen as well as growth performance compared to other treatments. From this research, it can be concluded that supplementation of NSP enzyme and organic chromium at doses of 1 g and 1 mg per kg of feed produces the best growth performance and anti-oxidant capacity in pomfret fish. Keywords: antioxidant, glycogen, growth performance, postprandial blood glucose ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efektivitas suplementasi enzim NSP dan kromium organik pada pakan ikan bawal Colosoma macropomum rendah protein untuk meningkatkan pemanfaatan karbohidrat. Pakan yang digunakan mengandung iso protein (20%) dan isoenergi. Rancangan faktorial dengan dua faktor yakni enzim NSP (0 dan 1 g/kg) dan kromium organik (0, 1, dan 2 mg/kg). Ikan bawal dipelihara dalam akuarium dengan ukuran 80 x 50 x 35 cm3 dan berisi air 75 L diisi 15 ekor ikan dan dipelihara selama 60 hari. Adapun parameter uji yang digunakan adalah pengukuran kadar glukosa darah postprandial, aktivitas antioksidan, glikogen, dan kinerja pertumbuhan. Hasil penelitian menunjukkan kadar glukosa darah postprandial tertinggi pada pemberian enzim NSP. Selanjutnya nilai malondialdehyde (MDA) terendah dan superoxide dismutase (SOD) dan glutathione peroxide (GPx) tertinggi didapat pada ikan yang diberi pakan mengandung pada 1 g/kg enzim NSP dan 1 mg/kg kromium organik. Ikan yang diberi pakan yang mengandung 1 g/kg enzim NSP dan 1 mg/kg kromium organik menunjukkan nilai signifikan pada glikogen hati dan otot serta kinerja pertumbuhan dibanding perlakuan lainnya. Dari penelitian ini dapat disimpulakan bahwa suplementasi enzyme NSP dan Kromium organik pada dosis 1 g dan 1 mg per kg pakan menghasilkan kinerja pertumbuhan dan kapasitas antioksidan terbaik pada ikan bawal. Kata kunci: antioksidan, glikogen, glukosa darah postprandial dan kinerja pertumbuhan

Effect of Kind and Dosage of Enrichment Materials on the Nutritional Quality of Rotifers Especially n3-HUFA Suprayudi, Muhammad Agus
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to evaluate the quality of rotifer enriched with four kinds of enrichement materials from the stand point of essensial fatty acids. Rotifer was enriched at 24 - 25oC for 18 hours at a density of 1000 ind/ml. Rotifers were treated by four kinds of enrichment materials such as oleic acid (R-OA), two different density of Nannochloropsis oculata, (4x107 and 16x107 cell/ml; R-N18 and R-N42) two different levels of eicosapentaenoic acids (EPA) triglyceride type (EPA-TAG) (20 and 40 ml/ml; R-E20, R-E40) and two different level of EPA ethyl ester (EPA-EE) (R-EE25 and R-EE50%) respectively. Rotifers enriched with Nannochloropsis oculata and EPA-EE type have a similar profile of essensial fatty acid especially on n3-HUFA that dominated by EPA, while DHA was in a trace amount or not detected. In addition Nannochloropsis oculata as an enrichment material showed the highest population density of rotifers during enrichment periods. Rotifer enriched with EPA-TAG has a more complete of essential fatty acid profile compared to other enrichment materials due to their contained both of EPA and DHA. We conclude that rotifer enriched with EPA-TAG as enrichment material showed the best nutritional quality of rotifers from the stand point of essential fatty acid. Key words : Rotifers, enrichment, eicosapentaenoic acid, docosaheksaenoic acid, n3-HUFA ABSTRAK Penelitian ini dilakukan untuk mengetahui profil asam lemak rotifera yang diperkaya dengan berbagai macam jenis dan dosis pengkaya. Rotifera dengan kepadatan 1000 ind./ml diperkaya dengan bebagai bahan pengkaya seperti asam oleat (R-OA); Nannochloropsis oculata dengan kepadatan 4x107 dan 16x107 sel/ml (R-N18 dan R-N42); eicosapentaenoic acid (EPA) tipe triglicerida (EPA-TAG) dengan dosis 20 dan 40 μl (R-E20 dan R-E40) dan EPA tipe ethyl esther (EPA-EE) dengan dosis 25 dan 50 μl per liter (R-EE25 and R-EE50%). Rotifera diperkaya selama 18 jam pada suhu 24-25oC. Rotifera yang diperkaya dengan Nannochloropsis oculata serta EPA-EE memiliki kesamaan profil asam lemak terutama pada incorporasi EPA, sedangkan DHA terkandung pada jumlah yang kecil atau tidak terdeteksi. Adapun rotifera yang diperkaya dengan EPA-TAG memiliki profil asam lemak yang lebih lengkap terutama ditinjau dari kandunga EPA dan docosahexaenoic acid (DHA) nya. Selanjutnya rotifera yang diperkaya dengan Nannochloropsis oculata memiliki populasi yang tertinggi dibanding perlakuan lainnya. Dari penelitian ini dapat disimpulkan bahwa pengkayaan dengan EPA-TAG memiliki kualitas rotifera yang terbaik ditinjau dari sisi kelengkapan kandungan asam lemaknya. Kata kunci : Kualitas rotifera, pengkayaan, asam eikosapentaenoik, asam dokosaheksaenoik, n3-HUFA

Growth and Feed Efficiency of Red Tilapia (Oreochromis sp.) Reared in Different Salinities Setiawati, Mia; Suprayudi, M. Agus
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The objective of this research was to know the effect of salinity on the growth and utilized of feed energy by red tilapia, Oreochromis sp. Four fishes with 4,15-4,42 g initial body weights were cultured in a 50x40x35 cm aquarium for 40 days. Fish were fed on these diets three times a day at satiation. Dietary growth rate, feed efficiency, protein and lipid retention increased with increasing salinity (p

Co-Authors , Alimuddin , Sarmin . Ridwan . Vera Dewi Mulia, Troef Soemarno A Halim A. Malik Serang A. Shofy Mubarak Achmad Noerkhaerin Putra Adang Saputra Adiasmara Giri, I Nyoman Agus Oman Sudrajat Ahmad Yazid Latif Aimma, Muhammad Ariful Akhmad Baihaqi Alimuddin alimuddin alimuddin Amelia Oktaviani, Amelia Anargya Pradipa Tristanandana Apriana Vinasyiam Aprilianti Pratiwi Arief Boediono Aris Sudiyanto Aris Tri Wahyudi Arradini, Dewi Artin Indrayati Asda Laining Asda Laining Atmojo, Joko Tri Azmi Afriansyah Benny Heltonika Burhanudin Faisal, Burhanudin D. Jusadi Dairun, Suclyadi DARMAWAN SETIA BUDI Darsiani Darsiani Darsiani Darsiani Deddy Yaniharto Deddy Yaniharto DEDI JUSADI Dedi Jusadi Dedi Jusadi Dedi Yaniharto dedy yaniharto Denny Wahyudi DIAH AYU SATYARI UTAMI Diamahesa, Wastu Ayu Dian Novita Sari Didi Humaedi Yusuf, Didi Humaedi Dinamella Wahjuningrum Dinar Tri Soelistyowati Dini Harianto Dodi Hermawan E. Mursitorini Eddy Supriyono Edy Marsudi Elas, Putri Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja Endriatmo Soetarto F. Hapsyari Faiqotul Himmah, Maihardiyanti Fatma Hajiali Fauzan Fredinan Yulianda Gadis Sri Haryani Gebbie Edriani Ghina Raudathul Jannah Giri Maruto Darmawangsa, Giri Maruto Hadi Pratama, Ricky Harton Arfah Hasan Abidin Hasan Nasrullah Hasan Nasrullah Hasrah Hasrah Helena Sahusilawane Hendriana, Andri Husnidar Husnidar I Dewa Putu Oka Suardi I Gede Setiawan Adi Putra I Gusti Agung Gede Wiadnyana I GUSTI AGUNG GEDE WIADNYANA . I Gusti Ayu Rai I MADE ARTIKA I Made Artika I Wayan Budiyasa I Wayan Nurjaya I Wayan Suanda I. Mokoginta Ichsan Achmad Fauzi Iis Diatin Imah, Nenden Imlani, Ainulyakin Hasan Inarto, Hendri ING MOKOGINTA Iqbal Kurniawinata, Mohamad Ira Akhdiana Irmawati Irvan Faizal Irzal Effendi Ismarica, Ismarica Jr., Muhammad Zairin Juli Ekasari Julie Ekasari Jullie Ekasari KATSUYUKI HAMASAKI KATSUYUKI HAMASAKI KETUT BUDI SUSRUSA Komang Adi Wika Wiguna Kusriyati Kusriyati L. Indriastuti Latifah Kosim Darusman Ligaya I. T. A. Tumbelaka M. Ikhsan M. Setiawati M. Tri Djoko Sunarno M. Zairin Junior Mas Tri Djoko Sunarno Meilisza, Nina Meritha, Wellya Wichi Mia Setiawati Muhaimin Hamzah Muhammad Jailani Muhammad Junior Zairin Muhammad Raditya Gumelar Muhammad Ridzky Adhory Muhammad Roikhan Amanullah Muhammad Safir Muhammad Zairin Jr Muhammad Zairin Jr Muhammad Zairin JR Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. MUHAMMAD ZAIRIN Jr. Muhammad Zairin Jr. Mulyasari Mulyasari Munawar Khalil Muslim Muslim Muzahar Niagara, Niagara Nina Meilisza Nur Bambang Priyo Utomo Nuraini Annisa, Nuraini Nuril Farizah Odang Carman Otie Dylan Soebhakti Hasan Padlilah, Rahmi Paramadina, Siwi Pattipeilohy, Christian Ernsz R Rifai R. Sutajaya Raditya Gumelar, Muhammad Rahminiwati, Min Rakhmawati, Rakhmawati, Ria Septy Anggraini, Ria Septy Ricky Ramadhan Ridhia Putri RIDWAN AFFANDI Rina Hirnawati Rizky Nafaida Samalei, Ermianus Sarwititi Sarwoprasojdo, Sarwititi Senja, Reza Karunia Setyorini, Catur Siti Komariyah Sri Nuryati Sri Nuryati Suardi Laheng Suci Antoro Suci antoro Suclyadi Dairun Sukenda, Sukenda, Supriyadi Hari Respati Suryadi Saputra T. Makmur Talita Shofa Adestia Tatag Budiardi Titin Kurniasih TOSHIO TAKEUCHI TOSHIO TAKEUCHI Tulas Aprilia Upmal Deswira Utomo, Nurbambang Priyo Wahyudi, Imam Tri Wasmen Manalu Wida Lesmanawati WIDANARNI WIDANARNI Widiyanto, Aris Wijianti, Hani Wijiwinarsih, Amallia Wika Wiguna, Komang Adi Winda Styani Irawan, Winda Styani Wiyoto Wiyoto Y. Hadiroseyani Yessy Rahmayanti Yogo Tri Saloko Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yutaka Haga Zairin Jr., Muhammad Zinatul Hayati

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