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Efektivitas Kombinasi Pemberian Minyak Ikan Lemuru (Sardinella longiceps) dan Aplikasi Hidroksiapatit terhadap Ekspresi FGF-2 pada Proses Bone Healing Tiaranita Ramadhani; Rima Parwati Sari; Widyastuti
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Bone healing on defect requiring the cells to form new bone. Fibroblast growth factor -2 belong to one of signaling control that play part in differentiation of fibroblast to osteoblast cell. Hydroxyapatite application on bone healing will aid bone regeneration as structure. Application of Sardinella longiceps oil that contain EPA and DHA as immunodulator could accelerate and optimize inflammatory phase in bone healing. Purpose: The aimed of this study to determine the effectivity of the combination of Sardinella longiceps oil and application of hydroxyapatite on the expression of fibroblast growth factor -2 (FGF-2) in bone healing process. Materials and Methods: The experimental units (male wistar rat, n=20) were devided into two groups, control (K) and treathment (P) groups. Treathment groups were given Sardinella longiceps oil per oral seven days before the surgery to make the defect, and continued for seven days later. Control groups were not given Sardinella longiceps at all. All of the wistar rat were have two defects in their right femur, which is use hydroxyapatite (K+ and P2) and no use hydroxyapatite (K- and P1). Defect was made as big as half diameter of round bur in femur. Rats were sacrificed seven days after surgery, and femur were sliced transversally and performed immunohistochemistry staining with Anti-bFGF Polyclonal antibody (Bioss®). Examination were done under light microscope. Data were analyzed using one-way Anova for FGF-2 expression. Result: Sardinella longiceps oil increased the mean of FGF-2 expression on P2 group : 15,29±5,251, compare to K- : 5,57±2,070 , K+ : 12,14±3,976 and P1 : 8,14±1,676 (P<0.05). Conclusion: The combination of Sardinella longiceps oil and hydroxyapatite application effective the expression of fibroblast growth factor-2 in the process of bone healing.
The Effect Of Sea Cucumber Powder And Hyperbaric Oxygen Therapy On The Expression Of Tumor Necrosis Factor Alfa In Rats With Diabetes Mellitus Induced By Porphyromonas gingivalis Caroline Prajnaparamitha Anggraini; Kristanti Parisihni; Widyastuti
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis have a bidirectional relationship with diabetes mellitus, both can increase oxidative stress that trigger an increase of pro-inflammatory cytokines, such as TNF-α. Sea cucumbers have anti-inflammatory component that act to inhibit the release of inflammatory mediators. Hyperbaric oxygen therapy reduce oxidative stress. Purpose: The aim of this study is to investigate the effect of the sea cucumber powder and hyperbaric oxygen therapy on the expression of TNF-α in rats with diabetes mellitus induced by P.gingivalis bacteria. Materials and Methods: The study was an experimental laboratories research with factorial design. Twenty wistar rats were divided into 5 groups. K0 negative control, K1 positive control. K1-K4 groups were induced with streptozotocin for diabetes condition and P.gingivalis bacteria for periodontitis condition. K2 group was treated with seacucumber 3% for 7 days, K3 with OHB 2.4 ATA 3 x 30’ interval 5’ for 7 days, and K4 group was treated with combination of sea cucumber and OHB. At the 51 st day all rats were sacrified, then the expression of TNF-α on periodontal macrophages were examined by immunohistochemistry stain. Data were analized by Kruskal Wallis followed by Mannwhitney. Result: Expression of TNF-α were increased in K1 (11.50±1.291) compare K0 (p<0.05). Sea cucumber treatment, OHB, and combination treatment decreased expression of TNF-α significantly in amount of 2.50±0.577 (K2), 8.25±2.217 (K3), 3.00±0.816 (K4). Conclusion: Sea cucumber powder and OHB therapy affected the expression of TNF-α in rats with diabetes mellitus induced by P. gingivalis bacteria.
The Role Of Calcium In Anadara granosa Shell Graft To Osteoblast Cells In Bone Healing Widyastuti
Denta Journal Kedokteran Gigi Vol 10 No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Angiogenesis and osteogenesis are pairs that occur in bone healing process. Blood vessels provide oxygen. Bone is constantly being remodelled process where osteoblasts are responsible for bone formation and osteoclasts for its resorption. Osteoblast function in the process of bone formation and an early activity can be seen in reparative bone healing phase. The use of Anadara granosa shell graft in which there are more calcium, is able to provide the same inorganic component of bone, and will influence osteoblast in bone healing. Purpose: To determine the effect of application Anadara granosa shell graft with content the large of calcium on the increased amount of osteoblast in the process of bone healing. Materials and Methods: Experimental animals used in this study were 21 male Wistar rats, which were than divided into 3 groups, namely the negative control group K(-), the positive control group K(+), and the treatment group P. In the negative control group without the application of graft, in the positive control group with the application of bovine graft and in the treatment group with the application of graft Anadara granosa on the femur rats. Results: Based on the result test showed a significant differences between K(-) with K(+), K(+) with P and K(-) with P. Conclusion: Calsium in Anadara granosa shell graft application effective to increased of osteoblasts in the process of bone healing.
Bioviabilitas Hidroksiapatit Ekstrak Cangkang Kerang Darah (Anadara granosa) Terhadap Sel Punca Mesenkimal Sebagai Bahan Graft Tulang Arlita Dewi Nastiti; Widyastuti; Fanny M Laihad
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: One of a bone defect fillers is ahydroxyapatite. Hydroxyapatite can be obtained from Anadara granosa shells extract that have high calcium content. The mineral content can be used as a bone filler material for bone grafting. However, the material is not known bioviability to against periodontal tissues so that was needed bioviability testing. This bioviability testing using mesenchymal stem cells as mesenchymal stem cells can differentiate into periodontal tissues. Purpose: To determine bioviability Anadara granosa shell extract on mesenchymal stem cells. Materials and Methods: This study was conducted using post-only control group design. Mesenchymal stem cells in 96 wells were divided into a control group of cells (n=7), media controls (n=7), and treatment (n=7). The treatment group were given various doses of the Anadara granosa shell extract with a concentration 54 mg/ml, 27 mg/ml, 13.5 mg /ml and 6.75 mg/ml. The mesenchymal stem cells were incubated for 24 hours before and after treatment. Once given MTT, the optical density is read by ELISA reader and calculated the percentage of viability. The cell viability data were analyzed with KruskalWallis statistical test, Mann-Whitney. Results: From the results showed that an increase in cell viability to Anadara granosa shell extract. Increased cell viability starting treatment group concentration of 54 mg/ml (23,,67%), 27 mg/ml (57,43%), 13,5 mg/ml (68,87%), 6,75 mg / ml (81,92%). The highest cell viability at concentrations of 6,75 mg/ml (81,92%). Conclusion: Bioviability extract blood clam Anadara granosa shell have the highestconcentration of 6,75 mg/ml and bioviabilitas lowest cell at a concentration of 54 mg/ml.
Daya Hambat Ekstrak Teripang Pasir (Holonthuria scabra) Terhadap Pertumbuhan Bakteri Mixed periodontopatogen In Vitro Susi Krestiana; Widyastuti; Vivin Ariestania
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis is caused by the occupation of gram-negative bacteria (mixed periodontopatogen) in oral cavity. Despite causing resistance, antibiotics are still used as a therapy for periodontitis. Sand sea cucumber (Holonturia scabra) extract hold somepotentials, if developed, to become the alternative therapy for periodontitis due to its antibacterial effects against gramnegative bacteria. Purpose: To determine sand sea cucumber extract (Holonturia scabra) in inhibiting the growth of mixed periodontopatogen bacteria. Materials and Methods: This study used a research design the post-test only control group design. 40 samples of BHI agar plates, contained mixed periodontopatogen bacteria samples, were divided into 5 groups samples. K1 (negative control) was given distilled water containing filter paper, K2 (positive control) was given the antibiotic tetracycline disc 30 g, while P1, P2, and P3 were given a filter paper containing sand sea cucumber extract with a concentration of 150 g/ml, 300 g/ml, and 450 g/ml. All sample from each group were anaerobically inoculated for 2x24 hours. After inoculation, the area aroundthe filter paper will appear to be clear and it will be measured with digital calipers (mm). The acquired data will be analyzed using the Kruskal-Wallis and Mann-Whitney test. Result: The inhibition rate of K1 (6,00 ± 0,00), K2 (28,975 ± 1,20049), P1 (6,8525 ± 0,30156), P2 (7,6237 ± 0,51281), and P3 (8,75 ± 0,3525). Conclusion: Sand sea cucumber extract (Holonthuria scabra) can inhibit the growth of Mixed periodontopatogen bacteria.
Translating Illocutionary Verbs of Quick Chat in Mobile Legends Bang Bang Dina Isnaeni Putriaji; Widyastuti
e-Journal of Linguistics Vol. 17 No. 2 (2023): July
Publisher : The Doctoral Studies Program of Linguistics of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/e-jl.2023.v17.i02.p07

Abstract

The global enthusiasm for online games requires the game developer andpublisher to provide various language translations. The players require tocommunicate and strategize well to win the match. The Quick Chat used byMLBB players to communicate and build strategy must be translated asappropriate to the context. This research investigated the translationtechniques applied to the Quick Chat verb in MLBB. Translation techniquesare important for transmitting the message from ST to TT. Since QuickChat primarily consists of verbs, this research focuses on the translation ofillocutionary verbs of Quick Chat in MLBB. This research appliedqualitative methods. Directive and assertive are the two illocutionary verbsfound in 30 randomly selected data. Furthermore, five translation techniquesapplied in the illocutionary verbs are the literal, transposition, amplification,modulation, and reduction technique. Additionally, this study also revealsthat the transposition technique changes the type of illocutionary acts fromdirective to assertive. Therefore, the selection of translation techniquesshould be appropriate to preserve the message and purpose of the QuickChat.
Permainan Edukasi Sigimbang Begesah: Literasi Pengenalan Aktivitas Fisik pada Anak Usia Dini di PAUD KB Al Hidayah Gasing Kabupaten Banyuasin Pratiwi, Rini; Kusumasari, Peni; Widyastuti
Jurnal Abdimas Mandiri Vol. 10 No. 1 (2026)
Publisher : UNIVERSITAS INDO GLOBAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36982/jam.v10i1.6329

Abstract

Aktivitas fisik merupakan komponen penting dalam mendukung tumbuh kembang anak usia dini, namun perubahan pola bermain akibat peningkatan penggunaan gawai menyebabkan menurunnya aktivitas gerak anak. Kegiatan pengabdian masyarakat ini bertujuan meningkatkan literasi aktivitas fisik dan keterampilan motorik anak usia 3–6 tahun melalui Permainan Edukasi Sigimbang Begesah di PAUD KB Al Hidayah Gasing, Kabupaten Banyuasin. Metode pelaksanaan meliputi literasi cerita edukatif melalui buku serial Sigimbang Begesah berjudul “Aktivitas Fisik”, permainan ular tangga edukatif, demonstrasi gerak sederhana, serta keterlibatan guru dalam integrasi aktivitas fisik ke dalam pembelajaran harian. Sebanyak 73 anak dan 6 guru berpartisipasi dalam kegiatan ini. Hasil menunjukkan peningkatan kemampuan motorik: 90% anak mampu mengikuti gerakan dengan benar, 93% menunjukkan peningkatan koordinasi tubuh, dan 96% mampu melakukan gerakan melompat dan berlari kecil secara stabil. Kegiatan ini juga meningkatkan pemahaman guru mengenai pentingnya aktivitas fisik serta strategi penerapannya di lingkungan PAUD. Program pengabdian ini membuktikan bahwa pendekatan literasi dan permainan edukatif efektif membangun kebiasaan hidup aktif serta mendukung perkembangan motorik, kognitif, dan sosial-emosional anak. Hasil ini menunjukkan bahwa pendekatan sederhana berbasis literasi dan permainan edukatif memiliki potensi besar sebagai intervensi efektif, berkelanjutan, dan mudah diadaptasi untuk membentuk perilaku hidup aktif sejak usia dini. 
Cultural Translation as a Means of Mediating Meaning in EFL Classrooms: A Systematic Literature Review Nur Alifah Sribudi Wahyuni; Widyastuti; Ali Mustofa
JADEs Journal of Academia in English Education Vol. 7 No. 1 (2026): JADEs Journal of Academia in English Education
Publisher : Tadris Pendidikan Bahasa Inggris IAIN Langsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32505/jades.v7i1.14796

Abstract

This study aims to analyze the role of cultural translation in bridging the gap between linguistic competence and cultural understanding in English as a Foreign Language (EFL) learning. The study employed a systematic literature review design with a qualitative approach, examining 27 articles from 2020 to 2025 obtained from Scopus, Web of Science, and Google Scholar through a screening process based on inclusion and exclusion criteria. Data were analyzed using reflective thematic analysis to identify key thematic patterns in the literature. The synthesis results revealed four main themes: linguistic and cultural gaps in EFL learning; cultural translation as a process of meaning negotiation; the pedagogical implications of cultural translation in EFL learning; and the challenges of its implementation in EFL classrooms. The research findings indicate that EFL learning needs to shift from a linguistically accuracy-based approach toward an interpretive approach that places meaning negotiation and cultural mediation at the core of intercultural communication. This study also repositions cultural translation not merely as a translation strategy or cultural instruction, but as a mechanism of meaning-making in language learning. Furthermore, this study proposes a conceptual model of cultural translation that places cultural interpretation and the negotiation of meaning at the core of EFL learning. Overall, this study demonstrates that cultural translation has the potential to serve as a new pedagogical and conceptual framework to support more reflective, contextual, and relevant EFL learning in the face of the multicultural and digital dynamics of global communication
HUBUNGAN ANTARA HARDINESS DENGAN BURNOUT PADA SANTRI PONDOK PESANTREN PERSATUAN ISLAM PUTRA BANGIL Abd Rochman Fahmi; Widyastuti
Jurnal Psikologi Poseidon Volume 1 Nomor 1
Publisher : Fakultas Psikologi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpp.v1i1.26

Abstract

Density of boarding activities demanding students to do more intense routines. These routines can be a pressure for students that experienced mental fatigue of Burnout. Burnout can be affected by external factors or internals. Internals factors become very important because individual assessnebt against external factors depend on its internal factors. The method of this research is quantitative correlational by using the boarding school’s students as subjects. Determination of the subject use proporsionate stratified random sampling. The method of data collection is spread the questioner wich contain Hardiness and Burnout scale based on aspects of each variables. This research analyze the relationship of Hardiness as internal factor with Burnout on boarding school’s students. This research use quantitative correlational method with a scale of Hardiness and Burnout that distribute on 97 boarding school’s students. The result of this research are calculated using the correlational product moment, then obtained a very significant result with a value of correlational is – 0,714. Based on the result , the correlation between Hardiness and Bunrout has proven with a negative direction of hypothesis. Based on simple regression analysis on the results, obtained F of 92,664 with sig = 0.000 which sig is less than 0.05. The effective contribution of Hardiness against Burnout is 50.5%, the meaning there is still a 49.5% chance of Burnout is affected by other variables.
PEMBERIAN EDUKASI PEMBIASAAN MENGGOSOK GIGI 21 HARI PADA SISWA SD AL AZHAR PROGRAM BULAN KESEHATAN GIGI NASIONAL 2024 “SCHOOL HEALTH PROGRAM”: PROVIDING EDUCATION ON THE HABIT OF BRUSHING TEETH FOR 21 DAYS TO STUDENTS OF AL AZHAR ELEMENTARY SCHOOL NATIONAL DENTAL HEALTH MONTH PROGRAM 2024 “SCHOOL HEALTH PROGRAM” Annariswati, icha Artyas; DA Retnowulan; Endah Wahjuningsih; Cane Lukisari; Arya Brahmanta; Emy Khoironi; Hansen Kurniawan; Eddy Hermanto; Hery Supriyadi; Meinar Nur Ashrin; MeraldaRsoyy; Widyastuti; Novendy Yoyada; Budi Santoso
Jurnal Pengabdian Masyarakat Pesisir VOLUME 4 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v4i1.136

Abstract

Oral health in Indonesia is still a health problem that needs attention. Based on the 2018 Basic Health Research (Riskesdas), it is reported that the prevalence of oral and dental problems in Indonesia is still very high. The above can be caused by the lack of knowledge and lack of awareness of the Indonesian population towards dental hygiene, to overcome oral health problems in Indonesia Training of Trainers for teachers and parents for 21-day morning and night brushing activities teaches children and adults about the benefits of good oral hygiene and shows them how to brush their teeth twice a day with fluoride toothpaste to realize caries-free Indonesia in 2030. This activity is held online through the zoom application. The target participants were elementary school and inclusive school students aged 7-12 years, 10 parents and 10 teachers at SD Al Azhar Kelapa Gading Surabaya. The mechanism of the activity is “Training of Trainer” (TOT) for students and parents, showing that Training of Trainer activities on teachers and parents of SD Al Azhar Kelapa Gading Surabaya students can increase their knowledge and awareness on oral health.
Co-Authors AA Sudharmawan, AA Aat Ruchiat Nugraha Abd Rochman Fahmi Abyan, Ramzi Ade Risna Sari Adesia Kurnia Tahir Agus Frianto Agustina Widianti Ahmad Munir Ahmad Ridfah Ali Mustofa Ali Mustofa Ali Saukah Almanda Meidynita Kadir Alvara, Kayla Mutiara Amalia Novarita Amri Mustaqim, Benor Anam Sutopo Andi Nur Annisa Andini, Pitaloka Andrian, Zulkifli Andro Ruben Runtu Anggraini, Dita Anindya, R.A.Sekar Ciptaning Aprilia Arfiansyah, Valdi Arlita Dewi Nastiti Arlita Gladys Tricia Charyadie Arya Ady Nugroho Arya Brahmanta Asnaniar, Wa Ode Sri Aulia, Danisya Ersadianis Aura Fitrah Aulia Awaluddin, Andi Sopy Bachmid, Sofyan Bambang Sumardjoko Bayu Prabowo, Puguh Benny Dhevyanto Brata, Raden Andalucija Rahmaniyya Budi Murtiasa Budi Santoso Cahyaningrum, Kartika Cane Lukisari Cane Lukisari, Cane Caroline Prajnaparamitha Anggraini Cholichul Hadi Cholifah , Siti DA Retnowulan Damar Prasmusinto Darsinah Destari Putri Chaerunnisa Dian Mulawarmanti Dian Rachmawati Afandi Dian Widya Damaiyanti Dina Isnaeni Putriaji dwi wahyono Dyah Ayu Retno Wulan Dyah Ayu Retnowulan Dyah Lestarining R Dyah Sulistyaningsih eddy hermanto Eko Ari Wibowo Emy Khoironi Endah Wahjuningsih Endang Fauziati Fahmawati, Zaki Nur Fajar Rezki Wahyuni Fani Pangabdian, Fani Fanny M Laihad Farra Dwi Susilo Wardhani Felicia, Ivana Fitria Rahmitasari Galih Mahardika Munandar Gangka, Nur Arrad Tenri Ghozali Rusyid Affandi Haerani Nur Hamim, Sumi Amariena Handayani, Budi Hansen Kurniawan Harnowo, Setyo Harsono Hasibuan, Anjani Vicka Prasasti Hery Supriyadi Hidayatullah Icha Artyas Annariswati Ida Betanursanti Ida Betanursanti Illahi, Ristu Adi Putri Ima Faizah Ismail Iwan Alim Saputra Jizaira Qaza Sanzabillah Harun Sally Jumiati Audina Rappan Karlina Samadi, Karlina Kemal Asyidiq, Muhammad Khafifah Indah Ramadhani Khoironi, Emy Kristanti Parisihni Kurniati Zainuddin, Kurniati Kurniawan, Hansen Kurniawan, Muhammad Fariez Kusumasari, Peni Lazuwardiyyah, Fairuz Maliki , Rendra Zainal Markhamah Marwani Saputri Meinar Nur Ashrin MeraldaRsoyy Moch. Bahak Udin By Arifin MONIKA TIARAWATI Muhamad Irfan Florid Muhammad Nur Hidayat Nurdin Muhammad Nur Wahyu Hidayah Muin, Suriati Abd. Murfiah Dewi Wulandari Nabila Fitri Rahmawati Nabila Putri Utami Nadia Asandimitra Haryono Nadia Azzahra Syamsuddin Narapati, Rama Aditya Natasya Azhara Mira Savira Nawangsari, Nur Ainy Fardana Nina Nilawati Nindria Untarini Nining Setya Ningsih Nisa, Alditta Khoirun Novendy Yoyada Novita, Yulia Nugroho, Him'mawan Adi Nur Akmal Nur Alifah Sribudi Wahyuni Nur Ashrin, Meinar Nur Ismy Latif Nur Octavia Nurfi Laili Nur‘ainingsih, Dyah Pamungkas, Krisna Adhi Patiroi, Andi Nurul Fadhilah Peni Kusumasari Pentatito Gunowibowo Pradani, Zalza Putri Pratama, Zikri Fatua Puspitasari, Anis Fadila Puspitasari, Elya Ika Putri, Febyani Amanda Rahman Abidin Rahmat Hidayat Rima Parwati Sari Rini Pratiwi RIRIN PUSPARINI Risma Fadhilia Arsy Rizqi, Additya Rosita Irmawati, Ari Sabar Narimo Salampessy, Maryam Saptaswari, Dianty Sari, Tanti Silvia Sayyidah Ummu Kalzum Septasari Cahya Nazhifah Septiawan, Darul SLAMET SETIAWAN Sulistyaningsih, Dyah Susi Krestiana Syamsulina Revianti Talitha Ayu Ramadhani Theofilus Tiaranita Ramadhani Ummu Hani Vivin Ariestania Wahyuningsih, Erfiana Watono Wibowo, Eko Ari Widaningsih Widianti, Agustina Widya Anggrianita Ramadhani Wulandari, Amalia Seha Ya'la , Zakira Raihani Yessy Artanti Zahira, Safna Tiara Zulkifli ZUMROTIN NISA