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Efek Terapi Sardinella longiceps Terhadap Tinggi Tulang Kortikal Mandibula Tikus Model Periodontitis Nur Octavia; Widyastuti; Dianty Saptaswari; Dian Widya Damaiyanti
Denta Journal Kedokteran Gigi Vol 13 No 2 (2019): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis begins with accumulation of pathogenic plaque containing bacteria and toxins. Subgingival bacteria found in chronic periodontitis are one of them is Porphorymonas gingivalis. Porphyromonas gingivalis bacteria causes progressive damage to the supporting tissues of the teeth, especially the alveolar bone. Alternative medicine that can be used is with herbal ingredients, namely Sardinella longiceps. Sardinella longiceps contains omega 3 (DHA and EPA) which inhibits proinflammatory cytokines thereby accelerating the process of bone regeneration. Objective: This study aims to determine whether the administration of gel (Sardinella longiceps) affects the height of the cortical bone of the mandibular periodontitis-induced rat Porphyromonas gingivalis.Research Methods: 25 male Wistar rats were divided into 5 groups. P0 is the normal group that is not treated. P + is a group induced by Porphyromonas gingivalis. P1, P2, P3 are the groups induced by Porphyromonas gingivalis and given Sardinella longiceps gel with concentrations of 10%, 20%, 40% respectively. On the 55th day all model mice were treated and mandibular cortical bone height was measured using a digital caliper in the interdental region of the 1st molar and left lingual molars from the apex of the alveolar bone to the mandibular base. The data obtained were analyzed by one way ANOVA followed by LSD (p = 0.05). Results: Statistical tests showed the mean height of mandibular cortical bone was P0 (5.21 ± 0.21), P + (4.67 ± 0.25), P1 (5.12 ± 0.14), P2 (5.51 ± 0.12), and P3 (5.88 ± 0.13). There was a significant difference (p <0.05) between treatment groups and positive control groups (p <0.05). Conclusion: Sardinella longiceps affects the height of the cortical bone of the periodontitis rats model induced by Porphyromonas gingivalis bacteria. The most effective in the group of sardinella longiceps gel with a concentration of 40%.
Bioviability of Mangrove Leaves Extract (Sonneratia alba) on Fibroblast BHK-21 Cell Culture Arlita Gladys Tricia Charyadie; Aprilia; Widyastuti
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Dental caries is a major oral health problem which is most commonly found in Indonesia. One of the main factors in role of causing dental caries is Streptococcus mutans which is the primary target of the prevention of caries. Leaf extract of Sonneratia alba has been proven to have an antimicrobial effect towards Streptococcus mutans, but its cytotoxic activity on normal cells is still unknown. The initial cell viability in this study was conducted using cell line BHK-21. Purpose: The aim of this study is to determine the cell viability of fibroblast cells line BHK-21 by given Sonneratia alba’s leaf extract. Materials and Methods: The experiment was conducted using post test only control group design. BHK-21 fibroblast cell culture on 96 wells were divided into cell control group (n=9), media control group (n=9) and test groups (n=9). The test groups were given various concentration of Sonneratia alba’s leaf extract, they were: 5%; 10%; and 20%. The cell cultures were incubated for 24 hours before and after treatment using Sonneratia alba’s leaf extract. After dripping MTT into the microplates, OD were read using ELISA reader and the viability were counted. The data that was acquired from the viability calculation was statistically analyzed using One Way ANOVA and LSD test. Results: Data showed the increase of viability cell in all the test groups. The highest viability cell showed by the test group with 10% concentration of Sonneratia alba (153,7122%±7,42458), and the lowest found in the test group with 20% concentration of leaf extract (141,4682%±7,48752). Conclusion: In this experiment, the leaf extract of Sonneratia alba proved to be nontoxic on fibroblast cells line BHK-21, the extract increased the viability and also caused proliferation of fibroblast cells.
(Study of Hyperbaric Oxygen Theraphy and Gold sea Cucumber (Stichopus Hermanii) 3% for Collagen Density of Rat’s with Diabetes Mellitus Induced by Porphyromonas Gingivalis Bactery Dyah Lestarining R; Dian Mulawarmanti; Widyastuti
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis is complication of DM in chronic state. Periodontitis susceptibility increase threefold in patient DM compared non-DM. Debridement with SRP alone is less effective in DM. Hyperbaric Oxygen Therapy (HBO) has the effect of increasing insulin sensitivity and synthesis of collagen. Collagen is the largest extracellular matrix supporting periodontal tissue. Stchopus hermanii contains condrotin sulfate serves to inhibit the formation of mixed periodontopatogen bacteri. Purpose: To determine the therapeutic effects of HBO 2,4 ATA 3x3’ interval 5’ 7 days and stichopus hermanii against periodontal ligament collagen density in the healing process of periodontitis with DM. Materials and Methods:The experiment design is factorial design. Rats are inducted with STZ 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis, 20 male Wistar rats were divided into 4 groups. K0 (control group), K1(STZ+P. gingivalis), K2(STZ+P. gingivalis+Stichopus hermanii), K3(STZ+P. gingivalis+HBO), and K4(STZ+P.gingivalis+stichopus hermanii HBO) after treatment on day 52, all groups were sacrificed and done making preparations for the HPA of the mandible bone. Results: Data were analyzed using chi-square, Kruskal wallis, and Mann Whitney U with 95% significance level (p = 0.05). increase level of collagen significantly K1(1 ± 0.00) - K2(2±0.00), dan K1(1 ± 0.00) - K4(2.5 ± 0.289).Conclution:Therapy HBO combination of 2.4 ATA 3 x 30 with 5 minutes air breaks for 7 days and gel Stichopus hermanii3% effective on the Densityof collagen in the periodontal ligament healing process periodontitis with DM.
Analisis Work Engagement Karyawan sebagai Program Kerja Mahasiswa Magang di PT. Baruga Asrinusa Development Marwani Saputri; Rahman Abidin; Widyastuti; Nur Akmal
Gudang Jurnal Pengabdian Masyarakat Vol. 4 No. 1 (2026): GJPM - Januari
Publisher : PT. Gudang Pustaka Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59435/gjpm.v4i1.1901

Abstract

Work engagement merupakan salah satu aspek penting yang mencerminkan keterlibatan karyawan secara fisik, emosional, dan kognitif dalam pekerjaannya. Penelitian ini bertujuan untuk memperoleh gambaran tingkat work engagement karyawan PT. Baruga Asrinusa Development pada tiga dimensi utama, yaitu vigor, dedication, dan absorption. Penelitian menggunakan pendekatan kuantitatif dengan desain deskriptif. Populasi penelitian adalah seluruh karyawan produksi yang berjumlah 45 orang, dengan penentuan sampel menggunakan teknik purposive sampling. Pengumpulan data dilakukan melalui penyebaran Utrecht Work Engagement Scale-17 (UWES-17) versi Indonesia yang terdiri dari tiga dimensi penilaian. Analisis data menggunakan statistik deskriptif melalui IBM SPSS untuk melihat nilai rata-rata, standar deviasi, dan persentase masing-masing dimensi. Hasil penelitian menunjukkan bahwa tingkat work engagement karyawan berada pada kategori sedang–tinggi, dengan nilai rata-rata skor total sebesar 46,53. Dimensi dedication menjadi aspek yang paling dominan, sedangkan vigor berada pada kategori moderat. Secara keseluruhan, hasil ini menunjukkan bahwa karyawan memiliki keterlibatan emosional dan kognitif yang cukup baik dalam menjalankan pekerjaan, meskipun masih terdapat ruang untuk peningkatan pada aspek energi dan ketahanan kerja. Temuan ini diharapkan dapat menjadi dasar bagi perusahaan dalam merancang strategi pengembangan sumber daya manusia untuk meningkatkan keterikatan kerja secara optimal.
Efektivitas Kombinasi Pemberian Minyak Ikan Lemuru (Sardinella longiceps) dan Aplikasi Hidroksiapatit terhadap Ekspresi FGF-2 pada Proses Bone Healing Tiaranita Ramadhani; Rima Parwati Sari; Widyastuti
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Bone healing on defect requiring the cells to form new bone. Fibroblast growth factor -2 belong to one of signaling control that play part in differentiation of fibroblast to osteoblast cell. Hydroxyapatite application on bone healing will aid bone regeneration as structure. Application of Sardinella longiceps oil that contain EPA and DHA as immunodulator could accelerate and optimize inflammatory phase in bone healing. Purpose: The aimed of this study to determine the effectivity of the combination of Sardinella longiceps oil and application of hydroxyapatite on the expression of fibroblast growth factor -2 (FGF-2) in bone healing process. Materials and Methods: The experimental units (male wistar rat, n=20) were devided into two groups, control (K) and treathment (P) groups. Treathment groups were given Sardinella longiceps oil per oral seven days before the surgery to make the defect, and continued for seven days later. Control groups were not given Sardinella longiceps at all. All of the wistar rat were have two defects in their right femur, which is use hydroxyapatite (K+ and P2) and no use hydroxyapatite (K- and P1). Defect was made as big as half diameter of round bur in femur. Rats were sacrificed seven days after surgery, and femur were sliced transversally and performed immunohistochemistry staining with Anti-bFGF Polyclonal antibody (Bioss®). Examination were done under light microscope. Data were analyzed using one-way Anova for FGF-2 expression. Result: Sardinella longiceps oil increased the mean of FGF-2 expression on P2 group : 15,29±5,251, compare to K- : 5,57±2,070 , K+ : 12,14±3,976 and P1 : 8,14±1,676 (P<0.05). Conclusion: The combination of Sardinella longiceps oil and hydroxyapatite application effective the expression of fibroblast growth factor-2 in the process of bone healing.
The Effect Of Sea Cucumber Powder And Hyperbaric Oxygen Therapy On The Expression Of Tumor Necrosis Factor Alfa In Rats With Diabetes Mellitus Induced By Porphyromonas gingivalis Caroline Prajnaparamitha Anggraini; Kristanti Parisihni; Widyastuti
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis have a bidirectional relationship with diabetes mellitus, both can increase oxidative stress that trigger an increase of pro-inflammatory cytokines, such as TNF-α. Sea cucumbers have anti-inflammatory component that act to inhibit the release of inflammatory mediators. Hyperbaric oxygen therapy reduce oxidative stress. Purpose: The aim of this study is to investigate the effect of the sea cucumber powder and hyperbaric oxygen therapy on the expression of TNF-α in rats with diabetes mellitus induced by P.gingivalis bacteria. Materials and Methods: The study was an experimental laboratories research with factorial design. Twenty wistar rats were divided into 5 groups. K0 negative control, K1 positive control. K1-K4 groups were induced with streptozotocin for diabetes condition and P.gingivalis bacteria for periodontitis condition. K2 group was treated with seacucumber 3% for 7 days, K3 with OHB 2.4 ATA 3 x 30’ interval 5’ for 7 days, and K4 group was treated with combination of sea cucumber and OHB. At the 51 st day all rats were sacrified, then the expression of TNF-α on periodontal macrophages were examined by immunohistochemistry stain. Data were analized by Kruskal Wallis followed by Mannwhitney. Result: Expression of TNF-α were increased in K1 (11.50±1.291) compare K0 (p<0.05). Sea cucumber treatment, OHB, and combination treatment decreased expression of TNF-α significantly in amount of 2.50±0.577 (K2), 8.25±2.217 (K3), 3.00±0.816 (K4). Conclusion: Sea cucumber powder and OHB therapy affected the expression of TNF-α in rats with diabetes mellitus induced by P. gingivalis bacteria.
The Role Of Calcium In Anadara granosa Shell Graft To Osteoblast Cells In Bone Healing Widyastuti
Denta Journal Kedokteran Gigi Vol 10 No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Angiogenesis and osteogenesis are pairs that occur in bone healing process. Blood vessels provide oxygen. Bone is constantly being remodelled process where osteoblasts are responsible for bone formation and osteoclasts for its resorption. Osteoblast function in the process of bone formation and an early activity can be seen in reparative bone healing phase. The use of Anadara granosa shell graft in which there are more calcium, is able to provide the same inorganic component of bone, and will influence osteoblast in bone healing. Purpose: To determine the effect of application Anadara granosa shell graft with content the large of calcium on the increased amount of osteoblast in the process of bone healing. Materials and Methods: Experimental animals used in this study were 21 male Wistar rats, which were than divided into 3 groups, namely the negative control group K(-), the positive control group K(+), and the treatment group P. In the negative control group without the application of graft, in the positive control group with the application of bovine graft and in the treatment group with the application of graft Anadara granosa on the femur rats. Results: Based on the result test showed a significant differences between K(-) with K(+), K(+) with P and K(-) with P. Conclusion: Calsium in Anadara granosa shell graft application effective to increased of osteoblasts in the process of bone healing.
Bioviabilitas Hidroksiapatit Ekstrak Cangkang Kerang Darah (Anadara granosa) Terhadap Sel Punca Mesenkimal Sebagai Bahan Graft Tulang Arlita Dewi Nastiti; Widyastuti; Fanny M Laihad
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: One of a bone defect fillers is ahydroxyapatite. Hydroxyapatite can be obtained from Anadara granosa shells extract that have high calcium content. The mineral content can be used as a bone filler material for bone grafting. However, the material is not known bioviability to against periodontal tissues so that was needed bioviability testing. This bioviability testing using mesenchymal stem cells as mesenchymal stem cells can differentiate into periodontal tissues. Purpose: To determine bioviability Anadara granosa shell extract on mesenchymal stem cells. Materials and Methods: This study was conducted using post-only control group design. Mesenchymal stem cells in 96 wells were divided into a control group of cells (n=7), media controls (n=7), and treatment (n=7). The treatment group were given various doses of the Anadara granosa shell extract with a concentration 54 mg/ml, 27 mg/ml, 13.5 mg /ml and 6.75 mg/ml. The mesenchymal stem cells were incubated for 24 hours before and after treatment. Once given MTT, the optical density is read by ELISA reader and calculated the percentage of viability. The cell viability data were analyzed with KruskalWallis statistical test, Mann-Whitney. Results: From the results showed that an increase in cell viability to Anadara granosa shell extract. Increased cell viability starting treatment group concentration of 54 mg/ml (23,,67%), 27 mg/ml (57,43%), 13,5 mg/ml (68,87%), 6,75 mg / ml (81,92%). The highest cell viability at concentrations of 6,75 mg/ml (81,92%). Conclusion: Bioviability extract blood clam Anadara granosa shell have the highestconcentration of 6,75 mg/ml and bioviabilitas lowest cell at a concentration of 54 mg/ml.
Daya Hambat Ekstrak Teripang Pasir (Holonthuria scabra) Terhadap Pertumbuhan Bakteri Mixed periodontopatogen In Vitro Susi Krestiana; Widyastuti; Vivin Ariestania
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis is caused by the occupation of gram-negative bacteria (mixed periodontopatogen) in oral cavity. Despite causing resistance, antibiotics are still used as a therapy for periodontitis. Sand sea cucumber (Holonturia scabra) extract hold somepotentials, if developed, to become the alternative therapy for periodontitis due to its antibacterial effects against gramnegative bacteria. Purpose: To determine sand sea cucumber extract (Holonturia scabra) in inhibiting the growth of mixed periodontopatogen bacteria. Materials and Methods: This study used a research design the post-test only control group design. 40 samples of BHI agar plates, contained mixed periodontopatogen bacteria samples, were divided into 5 groups samples. K1 (negative control) was given distilled water containing filter paper, K2 (positive control) was given the antibiotic tetracycline disc 30 g, while P1, P2, and P3 were given a filter paper containing sand sea cucumber extract with a concentration of 150 g/ml, 300 g/ml, and 450 g/ml. All sample from each group were anaerobically inoculated for 2x24 hours. After inoculation, the area aroundthe filter paper will appear to be clear and it will be measured with digital calipers (mm). The acquired data will be analyzed using the Kruskal-Wallis and Mann-Whitney test. Result: The inhibition rate of K1 (6,00 ± 0,00), K2 (28,975 ± 1,20049), P1 (6,8525 ± 0,30156), P2 (7,6237 ± 0,51281), and P3 (8,75 ± 0,3525). Conclusion: Sand sea cucumber extract (Holonthuria scabra) can inhibit the growth of Mixed periodontopatogen bacteria.
The Innovation of Cake Learning Application on Student’s Speaking Ability at Tenth Grade of Senior High School Istikhomahningsih, Titis; Widyastuti; Slamet Setiawan
IDEAS: Journal on English Language Teaching and Learning, Linguistics and Literature Vol. 13 No. 2 (2025): IDEAS: Journal on English Language Teaching and Learning, Linguistics and Lite
Publisher : Universitas Islam Negeri Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24256/ideas.v13i2.6105

Abstract

Cake is an Android-based app that focuses on learning English but keeps it fun. There are many benefits of using this app because this app is completely free. This app can practice speaking, reading and even listening skills. The purpose of this study is to find out whether there is a significant effect between students' speaking ability before and after teaching using the classroom cake learning application X Senior High School. This study uses quantitative research. The design of this study was pre-experimental with a pre-test design and a one-group post-test design. This study took place from March to May 2023 at Senior High School. To collect data, researchers used an oral monologue test to determine students' speaking ability. The population includes all 10th grade students of Senior High School, and this study used one class as the research sample using convenience sampling technique, whereby the total sample was 33 students. Additionally, pretest, treatment, and posttest were used to collect data. The results of data analysis show that the average score of students after being taught with the Cake learning application (78.24) is higher than before being taught with the Cake learning application. Learning application Cake episode (57,64). It can be concluded that there is an influence between students' speaking ability before and after being taught using the cake learning application in class X Senior High School.
Co-Authors AA Sudharmawan, AA Aat Ruchiat Nugraha Abyan, Ramzi Ade Risna Sari Adesia Kurnia Tahir Agus Frianto Agustina Widianti Ahmad Irsyad Ahmad Munir Ahmad Ridfah Ali Mustofa Ali Mustofa Ali Saukah Almanda Meidynita Kadir Alvara, Kayla Mutiara Amalia Novarita Amri Mustaqim, Benor Anam Sutopo Andi Nur Annisa Andini, Pitaloka Andrian, Zulkifli Andro Ruben Runtu Anggraini, Dita Anindya, R.A.Sekar Ciptaning Aprilia Apsari, Anindita Arfiansyah, Valdi Ariestania, Vivin Arlita Dewi Nastiti Arlita Gladys Tricia Charyadie Arya Ady Nugroho Arya Brahmanta Arya Brahmanta, Arya Asnaniar, Wa Ode Sri Aulia, Danisya Ersadianis Aura Fitrah Aulia Awaluddin, Andi Sopy Bachmid, Sofyan Bambang Sumardjoko Bayu Prabowo, Puguh Benny Dhevyanto Budi Murtiasa Budi Santoso Cahyaningrum, Kartika Cane Lukisari Cane Lukisari, Cane Caroline Prajnaparamitha Anggraini Cholichul Hadi Cholifah , Siti Damar Prasmusinto Darsinah Destari Putri Chaerunnisa Devy Maharani Putri Dian Mulawarmanti Dian Rachmawati Afandi Dian Widya Damaiyanti Dina Isnaeni Putriaji dwi wahyono Dyah Ayu Retno Wulan Dyah Ayu Retnowulan Dyah Lestarining R Dyah Sulistyaningsih eddy hermanto Eddy Hermanto Eko Ari Wibowo Emy Khoironi Endah Wahjuningsih Endang Fauziati Fahmawati, Zaki Nur Fajar Rezki Wahyuni Fani Pangabdian, Fani Fanny M Laihad Farra Dwi Susilo Wardhani Felicia, Ivana Fitri, Karimatul Fitria Rahmitasari Galih Mahardika Munandar Gangka, Nur Arrad Tenri Ghozali Rusyid Affandi Haerani Nur Hamim, Sumi Amariena Handayani, Budi Hansen Kurniawan Harnowo, Setyo Harsono Hasibuan, Anjani Vicka Prasasti Henry Kodrat, Henry Hery Supriyadi Hidayatullah Icha Artyas Annariswati Ida Betanursanti Ida Betanursanti Illahi, Ristu Adi Putri Ima Faizah Ismail Istikhomahningsih, Titis Iwan Alim Saputra Jizaira Qaza Sanzabillah Harun Sally Jumi Oktaviasari Jumiati Audina Rappan Kanigara Hawari Karlina Samadi, Karlina Kemal Asyidiq, Muhammad Khafifah Indah Ramadhani Khoironi, Emy Kholisoh, Titik Dewi Kristanti Parisihni Kristina Sihaloho, Kristina Kurniati Zainuddin, Kurniati Kurniawan, Hansen Kurniawan, Muhammad Fariez Kusumasari, Peni Laili, Nurfi Lazuardi Fatahillah Hamdi Lazuwardiyyah, Fairuz Maliki , Rendra Zainal Manaf, Ahnaf Markhamah Marwani Saputri Meinar Nur Ashrin MeraldaRsoyy Moch. Bahak Udin By Arifin MONIKA TIARAWATI Muhamad Irfan Florid Muhammad Nur Hidayat Nurdin Muhammad Nur Wahyu Hidayah Muin, Suriati Abd. Murfiah Dewi Wulandari Nabila Fitri Rahmawati Nabila Putri Utami Nadia Asandimitra Haryono Nadia Azzahra Syamsuddin Narapati, Rama Aditya Natasya Azhara Mira Savira Nawangsari, Nur Ainy Fardana Nina Nilawati Nindria Untarini Nining Setya Ningsih Nisa, Alditta Khoirun Novendy Yoyada Novita, Yulia Nugroho, Him'mawan Adi Nur Akmal Nur Alifah Sribudi Wahyuni Nur Ashrin, Meinar Nur Ismy Latif Nur Octavia Nur‘ainingsih, Dyah Pamungkas, Krisna Adhi Patiroi, Andi Nurul Fadhilah Peni Kusumasari Pentatito Gunowibowo Pradani, Zalza Putri Pratama, Zikri Fatua Pratiwi, Safira Agnes Puspitasari, Anis Fadila Puspitasari, Elya Ika Putri, Febyani Amanda Rahman Abidin Rahmat Hidayat Ramanindia, Andiani Rima Parwati Sari Rima Parwati Sari, Rima Parwati Rini Pratiwi RIRIN PUSPARINI Risma Fadhilia Arsy Rizqi, Additya Rosita Irmawati, Ari Sabar Narimo Salampessy, Maryam Saptaswari, Dianty Sari, Maharani Dewinta Sayyidah Ummu Kalzum Septasari Cahya Nazhifah Septiawan, Darul SLAMET SETIAWAN Sulistyaningsih, Dyah Susanto, Michelle Rosalind Susi Krestiana Syafira Devi Anita Syamsulina Revianti Talitha Ayu Ramadhani Taufik, Giri Theofilus Tiaranita Ramadhani Ummu Hani Vivin Ariestania Wahyuningsih, Erfiana Watono Wibowo, Eko Ari Widaningsih Widianti, Agustina Widya Anggrianita Ramadhani Widyasri Prananingrum Wulandari, Amalia Seha Ya'la , Zakira Raihani Yani Sylvania Yessy Artanti Yusuf, Diajeng Ayu Permatasari Zahira, Safna Tiara Zulkifli ZUMROTIN NISA