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PUDJI ASTUTI

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CORRELATION OF PROGESTERONE AND CORTISOL PLASMA LEVELS BETWEEN OVULATED AND NON-OVULATED ETTAWA CROSSBREDS DOES Pudji Astuti; Diah Tri Widayati; Sunendar -; Kresno Suharto; Asmarani Kusumawati; Aris Junaidi
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This experiment was conducted to determine the correlation of progesterone and cortisol levels in plasma between ovulated and non-ovulated Crossbreds Ettawa does. Eight does were used in this experiment and they were divided equally into 2 groups, i.e. group I consisting of 4 normal ovulated does with the average body weight of 37.5 ± 3.109 kg and group II consisting of 4 non-ovulated does with the average body weight of 28 ± 2.160 kg as group II. The estrus cycles of all does were synchronized using intravaginal device of controlled internal drug release (CIDR) accompanied by intra-muscular injection of prostaglandin F2 (PGF2) alpha. Immediately after the onset of estrus, blood samples were collected from jugular every 3 and 6 hours for 72 hours after onset of estrus. The concentration of cortisol and progesterone in plasma was assayed by enzyme linked immunosorbent assay (ELISA). The results showed that the average concentrations of cortisol in ovulated does (90.89±26.22 ng/mL) was higher than in non-ovulated does (42.70±37.18 ng/mL). Similarly, the concentrations of progesterone in ovulated does (0.098±0.0423 ng/mL) was higher than in non-ovulated does (0.093±0.056 ng/mL). It was evident that the change in progesterone level was closely associated with the change in cortisol level in plasma.

Physiological Response of Bligon Buck to Transportation: Relation to Level of Thyroid Hormone Pudji Astuti; Sarmin -; Asmarani Kusumawati; Claude Mona Airin; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Transportated animals may subject to a variety of physical stimuli including metabolism, crowding,noise, handling, isolation, agitation, and extreme temperature .The aim of this study was to determine thechanges of serum T4 and T3 concentration, during animals transportation. Six adult Bligon buck with bodyweight ranging from 26-30 Kg were used in this study. Two weeks prior to the experiment, the animalswere given anthelmintic Albendazole to eliminate egg worm. All animals were fed standard diet in theirpen at 10% of their body weight per head daily and commercial concentrate also given everyday. Freshwater was provided ad libitum. All animals were transported around village for 16 hours starting from18.00 pm until 10.00 am in open small truck (3 x 2 m); eye contact each others would be possible. Bloodsamples were withdrawn from jugular vein using vacutainer tubes containing heparin into 1.5 mL glasstubes, then centrifuged at 500 g for 15 minutes. Plasma was collected to be stored at –20° C. The bloodwere collected every 4 hours from 8 hours before transportation ( at 10.00 am, 14.00 pm and 18.00 pm)until the time of arriving after transportation at 10.00 am. Plasma was harvested and stored at –20° Cuntil T3 and T4 concentrations were measured using ELISA method (enzyme linked immunosorbentassay) product DRG, Germany. The result showed that transportation of Bligon bucks for 16 hours havean affect on level of T4 only (P<0.05) and not for T3 concentration (P>0.05) due to physical stimuli such ascrowding, heat stress, noise, handling would be discarded so that the metabolic process was stable. Duringtransportation, decreasing of T4 levels indicated conversion of T4 to T3 to form active hormone.

Pemanfaatan Electronic Nose sebagai Sensor Kimiawi Urin Guna Melacak Birahi Sapi (ELECTRONIC NOSE AS URINARY CHEMICAL SENSOR FOR DETERMINING ESTROUS PHASE IN CATTLE) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Luthfiralda Sjahfirdi; Hera Maheshwari
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The timing of artificial insemination relies largely on behavioral observation of estrus. The problemfaced is that not all cattle shows signs of estrus significantly, which affect the accuracy of insemination,and therefore,the success rate of Artificial Insemination is less than 50%. Recently, the determination ofestradiol levels as an indicator of estrus is done by observation of physical signs and doing ELISA test,which is expensive and provide longer time. In order to solve these problems, a tool estrus detector is madenamely Electronic Nose (EN). Determination of estrus with EN is cheaper because it does not need to usecomplexmaterials, just only use the samples. Mechanism of action of EN is using a sensor that is vaporized,while animals estrus will emit pheromones that are vaporized. Theaim of this study was to determinewhether the stage of estrus can be detected by using EN. Urine of female Ongole Crossbred which maintainedin Kuwang, district of Cangkringan, Yogyakarta, with BCS of 3 was used in this research. The sample wascollected shortly before injection of dinoprost as estrus synchronization then it repeated when cattle got estrus phase. The urinary sample of the estrus cattle was sensitive to methane, propane, butane, whereasin non-estrus cattle, besides the three of these component (methane, propane, butane), sensor was alsocaptured hydrogen sulfide. Furthermore, our electronic nose had been able to distinguish estrus phase andnon-estrus based on domain area.Thus, the Electronic Nose is very prospect used as a detector of estrus incattle. Hydrogen disulfide could possibly be used as an indicator comparison between cow estrus and nonestrus.

Kadar Estradiol dan Progesteron Serum, Tampilan Vulva dan Sitologi Apus Vagina Kambing Bligon Selama Siklus Birahi Irkham Widiyono; Prabowo Purwono Putro; Sarmin -; Pudji Astuti; Claude Mona Airin
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was carried out to determine the serum estradiol and progesterone concentration, vulvaappearance, and vaginal cytology during estrous cycle in bligon goat. Four female non pregnant bligongoats (Indonesian indigenous) were used in this research. They were clinically healthy and showed normalestrous cycle. Estrous state was examined by using biological method. When the animals showed acceptanceto be mounted by a buck, they were confirmed as the first day of estrous cycle (estrous phase). Collectionof blood sample, preparation of vaginal smears, and measurement of some physical genital parameterswere conducted by standard veterinary clinical methods at day 11, day between 3-5, day between 6-16, andday between 17-19 of estrous cycle. Enzyme linked immunosorbent assay (ELISA) was used to determineserum progesterone and estradiol concentrations. Mean difference of each parameter was analyzed byanalyses of variance (Anova), followed by Tuckey HSD. The serum estradiol concentration ranged between211.25-247.77 pg/ml. Peak of the estradiol was occurred at the first day of estrous phase, namely 247.77pg/ml and to decrease at day 3-16. The serum progesterone concentration was at the level of 0.21-0.70 ng/ml and showed a significant increase at day 6-16 (p<0.05). The specific physical genital changes (swellingvulva, reddish mucous membrane of vulva, and excretion of viscous transparent vaginal discharge) wereidentified as the animals were at the estrous phase. Composition of exfoliative vaginal cytology did notshow any specific and consistent changes throughout the estrous cycle. Superficial and intermediate cellswere very dominant (>60%) during estrous cycle. In conclusion, serum estradiol, progesterone, the vulvaappearance combined with sexual receptivity could be a good estrous predictor for female cycling bligongoat.

Restriksi Pakan yang Diikuti dengan Refeeding Menurunan Level Fosfat Inorganik dan Kalsium pada Kambing Kacang Jantan Dewasa (FEED RESTRICTION FOLLOWED BY REFEEDING DECREASED THE INORGANIC PHOSPHATE AND CALCIUM LEVELS IN ADULT KACANG GOATS) Sarmin Sarmin; Irkham Widiyono; Pudji Astuti; Prabowo Purwono Putro
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objective of this research was to evaluate metabolism of inorganic phosphat (Pi) and calcium (Ca) in kacang goats subjected to 60-d feed restriction and 60-d full feeding (refeeding). Nine adult male goats were divided into three groups used in this study. Restrictions include 40% (group I), 50% (group II), and 60% (group III) of the ration of dry fodder. To obtain the baseline all animals from all groups were fullfeeding from the start of the study (day 0) to day 60 then followed by restriction stage on day 61 to day 120. The next stage is refeeding from day 121 to 180. Pi and Ca levels were analyzed on days 60, 120, and 180, respectively. The results showed that restriction followed by refeeding decreased Ca levels in all three groups and Pi of animals in groups II and III was below the baseline. The level of Pi reach above the baseline were seen only in animals in group I following restriction and refeeding, although there was a tendency to decrease after refeeding. Based on the results of the study it is concluded that the restriction followed by refeeding decreases the level of Pi and Ca in adult male kacang goats. ABSTRAK Tujuan penelitian ini adalah mengkaji metabolisme fosfat inorganik (Pi) dan kalsium (Ca) selama 60 hari restriksi (pembatasan) pakan yang diikuti dengan 60 hari pemberian pakan kembali secara fullfeeding (refeeding) pada kambing kacang jantan dewasa. Sembilan ekor kambing kacang jantan dewasa terbagi menjadi tiga kelompok digunakan dalam penelitian ini. Restriksi meliputi 40% (kelompok I), 50% (kelompok II), dan 60% (kelompok III) dari 3,5% bahan kering dari bobot badan. Tahap awal sebagai baseline dimulai dengan pemberian pakan fullfeeding pada hari ke-0 sampai hari ke-60 dilanjutkan dengan tahap restriksi dimulai pada hari ke-61 sampai hari ke-120. Tahap berikutnya adalah refeeding mulai hari ke-121 sampai dengan hari ke-180. Level Pi dan Ca dianalisis pada hari ke-60, 120, dan 180. Hasil penelitian menunjukkan bahwa restriksi yang diikuti refeeding menurunkan level Ca pada ketiga kelompok dan Pi kelompok II dan III di bawah baseline. Level Pi berada di atas baseline hanya pada kelompok I setelah restriksi dan refeeding, meskipun cenderung menurun setelah refeeding Berdasarkan hasil penelitian disimpulkan bahwa restriksi yang diikuti dengan refeeding menurunkan level Pi dan Ca pada kambing kacang jantan dewasa.

Profil Saraf Nitrergik Sekum Ayam Pedaging yang Diinfeksi Eimeria tenella (THE PROFILE OF NEORAL NITRERGIC IN THE COECUM OF BROILER INFECTED WITH Emeria tenella) Amelia Hana; Pudji Astuti; Yuda Heru Fibrianto; Sarmin .; Claude Mona Airin
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Neurotransmitter found in the enteric nervous system that plays an important role in a variety ofenteropathies, including inflammatory bowel disease. Alteration of nitrergic neurons has been reported to bedependent on the manner by which inflammation is caused. This study was performed to determine theprofile of neural nitrergic with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d)staining in the cecum of broiler infected by Eimeria tenella (E. tenella). Thirty six health free of coccidiosisbroiler one-day old chickens (DOC) used in this study. All of broiler adapted for 7 days with food and drinkgiven ad libitum then divided into three groups, each group consisted of 12 broilers. The 1st group wascontrol only given orally 1.0 ml of distilled water/ heads , while 2nd group was infected with a single dose of5x103 oocysts/ head orally, and 3rd group was infected with a single dose of 2x104 oocysts/ head orally. Onday 7 post infection, all of chickens were fasted for 12 hours then were euthanized and cecum was taken.Lesion score of cecum was assessed. Furthermore, tissue of the coecum was prepared for hisochemicalusing NADPH-d staining to determine morphology, and morhometric of nitrergic neurons. The resultshown that cecum lesion score of group I is 0, group II is +2, group III is +3. Neuron density in the cecum ofgroup I is 2794.96+4.65 neuron/cm2, group II is 4930.36+63.73 neuron/cm2 and group III is 7892.31+44.97 neuron/cm2. Neuron density of group II and III increased signifcantly (p<001) than group I. Nitrergic neurondesity of group III increased significantly (p<0.05) than that of group II. It was concluded that the infectionof E.tenella led to increase the number of neutrl mienteric nitrergic of the cecum. The higher lesion score ofcecum led to increase the number of mienteric nitrergic neuron.

Co-Authors . Harimurti . Sumartono A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA ABID BUDIMAN, ZAKI Achmad Gunadi Adi Sucipto, Adi Afandi, Muhammad Arif Affandi, Mohamad Arif Agung Sasongko Agustiana Putri, Erwinda Dekaria Agustin Wulan Suci Dharmayanti Agustin, Eny Widhia Agustin, Lidya Ayu Ahmad Farhan, Ahmad AHMAD HAFIDZ JIMMY PRASETYO Ahmad, Gelar Ali Aimim-Tham, Sukanya Airin, Claude M. Alfarisa Nururrozi Amal Witonohadi Amalia Marom, Amalia Amandia Dewi Permana Shita Ambarsari, Nila Amelia Dewi, Elyna Amelia Hana Amelia Hana Amelia Hana Amelia Hanna Amin, Izat Rolibi Putra Aminah Aminah Amir Husni Amrullah Anindito Amrulloh, Dimas Syahrul Andhika Dwi Nugroho Andriyani Astuti ANGESTI RAHAYU, DIAN Aranggraeni, Renda Arda Ariani Ardhana, Mahardhika Achmad Ardyan, Fitrada Ridlo Aries Andjar Sulistyono Aris Junaidi Arnes Faradilla Asih Kuswardinah Asmarani Kusumawati Astuti, Endah P. Asyhari . Atik Kurniawati Atmojo, Tomy Tri Audito, Yudhistira Yovi Azzahra, Novianti Sekar Ayu Bachrul Amiq Badru Tamam, Ahmad Agus BAMBANG SUGENG SURYATNA Bambang Sugeng Suryatna, Bambang Sugeng Baskoro, Moch Idham Betha Ariandini Bin Awang, Mohd Badrol Borman, M. Syahrul Cahyanti, Selvy Calista, Winna Dhara Calude Mona Airin Cholis S, Bambang Claude Mona Airin ClaudeM Mona Airin Dadan Umar Daihani Dadang Surjasa, Dadang Danang Dwi Saputro Dedy Sugiarto Dewi Erowati, Dewi Diah Tri Widayati Dian Mardi Safitri Didien Suhardini Didik Wahyudi DIKA CHANDRA, WIDYA Dina Mutia Sari1 Divani, Adelia Lintang Diyah Ayu Sari Arum Dasih Docki Saraswati Dondin Sajuthi Dorina Hetharia Dwi Budi Santoso DWINDA SAPUTRA, ADHITYA Dyah Nurani Setyaningsih Emelia Sari EMMILIA RUSDIANA, EMMILIA Erganto Jai, Alfansyi Maximilano ERIC HAYES Erna Sulistyani Ernie Maduratna Setiawatie Fajar Yuliawan Fanino Jati Maulana, Fanino Jati Farhan Firdiansyah Fathin, Sulafah Fauziyah, Atika Nur Febryanti, Ikka Ferdinal, Yan Fijay Sakti Pranata Fitriyah Fitriyah FRISTANTO, WIRANDA Gabriella Mistissy Galau, Argha Sirna Gemilang, Putri Surya Gesa Bimantara Gumilar, Mikhael Cahyo GUSTI AYU KOMANG NOVIANI, I Habyba, Anik Nur Hakim, Muhammad Abdul handoyo, setyo medy Hanum, Maulida Selma Harahap, Elfira Febriani HEMALINI HARAHAP, BUTET Hendra Setyo Haryadi Hendy Hendarto HERA MAHESHWARI Hermawati, Rr. Lilis Hilmi , Hafidz El I Gede Adhitya Wisnu Wardhana I GUSTI AYU KOMANG NOVIANI Idriwal Mayusda Iin Eliana Triwahyuni Indah Permata Sari Indiati Indiati, Indiati Indra Surjati Indriani, Rizka Intan Muchtadi Alamsyah Ira Larasati Irkham Widiyono Ismaya (Ismaya) isnaini, surya novinda Iveline Anne Marie Iwan Purwanto Jimny Suryo Pamungkas Kholidiya, Wulan Ratna Nur khusnul khotimah Koko Kurniawan Konara, Thamasi Kresno Suharto Kusniyanti, Elvira Laili Rachmawati Laiyan, Macaria Theresia Larasati Rizky Putri Lelana, Madya Daka Lestari, Alda Tri Lutfia, Eka LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji Lydia Sari M. Khoirul Anam Mahardhika, Vita Malikah, Ratu Keysha Marie Curie Habibah Maulida Selma Hanum Maulidya , Rahmi Meddiati Fajri Putri MEGA DEWI KARTIKA Merina, Bresca Michael Setiawan Miftah Farid Milanisa, Septia Atma millah, lutfia widatul Mimi Salmawati MOHAMMAD ALI FIKRI Mohammad Ali Wafa Mose, Windi Muflikhah, Dewi Muhamad, Fajar muhamad Muhammad Ansori Muhammad Ilham Nafila, Dwita Nanik Setyowati Nikki Aldi Nikki Aldi, Nikki Nilla Nilla Nilla, Nilla Nora Azmi Novia Rahmawati Novyanti, Heny Nugroho, Haryo Yudhistira Hamengku Nugroho, Mohammad Alvian Adi Nur Azizah, Syifa Nadia Nuraini Rahmawati Nurmasitah, Sita Nurul Hikmah Nurvina Septi Adifa Octavianti Paramita Oktadina Sekarwangi Oktaviani, Salsabila Pane, Muhammad Alwiadi Pangestu, Qori Y. Paramita, Nenci Yuniar Pardede, Sarah Berliana Parwadi Moengin Parwadi Moengin Penny Humaidah Hamid Permata, Dea Permatasari, Sabania Dinar Prabowo Purwono Putro Prabowo Purwono Putro Pradika, Alvin Pradityo Yoga Wibowo Prameswari, Galuh Ayu Prasetyaningrum, Andania Ulfa Yuga Prasetyowati, Fitria Nur Purwoko . Puspoayu, Elisabeth Septin Putri Krida Gita P. Putri, Sendy Uda Cantika R. Sapto Hendri Boedi Soesatyo Rachmadi, Moch Faizal Rachmania, Adinda Hilda Raden Ilham Akbar Ramadhan, Muchamad Firman Ramadhani, Anindya Ramadhani, Mitha Annisa Ramadhani, Poppy Sagita RAMADHANI, WISNU Ramadhani, Yudhistira Wahyu Ramadhanti, Wita Ramadhanti Ramadhanty, Debby Rarastoeti Pratiwi Ratna Dewanti, I Dewa Ayu REFTI HANDINI LISTYANI Rendra Chriestedy Prasetya Respitawulan, R Ririn Setyowati Risa Ummami RIZKA LUTFITANINGTYAS Rochmah, Jayanti Astridya ROHMAWATI, SITI ELVINA Rosagita, Anindya Aurin Rosandra, Befiria Meike Rosita Dwiana Prayudawisi Rositayanti Hadisoebroto RUHKAYAH, SITI Rujianto, Vinanda Ayu Putri Sakinata, Ariyanti Lady salafi, syafara alifia Sally Cahyati Salsabilla, Nasywa Saptariana Saptariana - saputri, vista purnama Saragi, Simon Jansen Sari Setyaningsih Sarmin - Sarmin . Sarmin Sarmin Sarmin Sarmin Savira Aulia Rachim SEPTIK PRIANDA, NISYA Setyaningsih, Sari Shanti, Elvika F. A. Shelina Putri Kurniadi Shihsalamadhina , Fahma Sihombing, Berkam Triputra Tulus Pangidoan Siti Fathonah Siti Hawa Siti Maghfiroh Siti Qotimah Situmorang, Marwan Bilton Slamet Widiyanto Soemanto, Rizki Anggerda Lopez Sulistyowati . Sunendar - Suroya, Zahrotus Susilo, Agung Hendro Swandari, Rindang Syah Alam Syah Putri, Veronica Vidia Syah, Muhammad E. Syahran Wael Taufik, Moh Teguh Budipitojo Tiena Gustina Amran Tiena Gustina Amran, Tiena Tri Sunarsih Tri Wahyu Pangestiningsih TRIANTO, HIZKIA Triwulandari Satitidjati Dewayana Triwulandari SD, Triwulandari Tuty Laswardi Yusuf Ummah, Maslakhatul Laila Utami, Gayung WAHYUNING RATRI, ROOSDIANA PUSPITA Wahyuningsih Wahyuningsih Widianti, Alifia Widodo, Alaya Dwi Salvahira Wijaya, Lutfi Winnie Septiani Winnie Septiani Yojana, Ratna Mira Yuda Heru Fibrianto Yuli Kurnia Ningsih Yuliana, Erika Yuliana, Fenty Dwi Yuneldi, Rizki Fitrawan Yunianti, Ervisya Nandya Zahara Meilawaty Zahra, Nafisa Az zain, rona suroya Zidan, Muhammad Faris

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