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Peningkatan Titer Antibodi Terhadap Avian Influenza Dalam Serum Ayam Petelur yang Divaksin Dengan Vaksin Komersial Ummu Balqis; Muhammad Hambal; Mulyadi Mulyadi; Samadi Samadi; Darmawi Darmawi
Jurnal Agripet Vol 11, No 1 (2011): Volume 11, No. 1, April 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v11i1.647

Abstract

Increasing of antibody titre against avian influenza in serum of vaccinated laying hens with commercial vaccineABSTRACT. The advantages of vaccination are that it reduces the risk of infection, and concurrently reduces morbidity, mortality and shedding of virus. The goal of the present study was to evaluate efficacy of Avian Influenza commercial vaccine based on humoral immunity responses of laying hens. Totally, 20 breakel silver layer hens were used in this research. The laying hens were vaccinated using Avian Influenza commercial vaccine (H5N1). Blood samples were collected from the axilaris vein (left or rigt) one time at prevaccination and repeated three times with an interval of one month at postvaccination. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that Avian Influenza commercial vaccine (H5N1) was a good protection because the vaccine was able to trigger protective humoral immunity of laying hens indicated by increasing of antibody titre in serum of vaccinated laying hens during three months.
Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli Darmawi Darmawi; Ummu Balqis; Risa Tiuria; Muhammad Hambal; Samadi Samadi
Jurnal Agripet Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v10i2.638

Abstract

Purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli L3 larvae stageABSTRACT. The main immunoglobulin fraction of poultry is called IgY, in order to distinguish it from the mammalian IgG. This article focus on purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli larvae to obtained purity IgY. Active vaccinations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 g. The vaccinations were repeated three times with dose of each 60 g with an interval of one week. The first vaccinations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by agar gel precipitation test (AGPT). The chickens eggs were collected from 49 day after vaccinations. IgY was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ekscretory/secretory IgY protein was determined by Bradford method ( = 280 nm). The result showed that antibody in yolk was begun detect with AGPT at four weeks after vaccination. IgY concentration after purification was 0,875 0.251 mg/ml. This study has shown that the product released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing antibody through yolk as biologic manufacturer could be a good choice.
Kajian Titer Antibodi Pada Yolk dari Ayam yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli Darmawi Darmawi; Ummu Balqis; Risa Tiuria; Muhammad Hambal; Samadi Samadi
Jurnal Agripet Vol 8, No 2 (2008): Volume 8, No. 2, Oktober 2008
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v8i2.611

Abstract

Evaluation of antibody titre in yolk from immunized chickens with excretory/secretory antigen of L3 stage of Ascaridia galliABSTRACT. The purpose of the present study was to trigger humoral immunity of chickens egg yolk exposed to excretory/secretory released in vitro by L3 stage of Ascaridia galli. Amount of 6 head chickens were divided into two groups. First group, the chickens were not immunized. Second group, the chickens were immunized with excretory/ secretory. Active immunizations with excretory/ secretory antigen were applied intra muscularly of chickens with an initial dose of 80 g. The immunizations were repeated three times with dose of each 60 g with an interval of one week. The first immunizations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by enzyme linked immunosorbant assay (ELISA). The result showed that antibody in yolk was begun detect with ELISA increased at two weeks after immunization, This study has shown that the excretory/secretory released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing IgY in yolk.
Perubahan Patologi Anatomi Hati dan Saluran Empedu Sapi Aceh Yang Terinfeksi Fasciola gigantica Ummu Balqis; Darmawi Darmawi; Siti Aisyah; Muhammad Hambal
Jurnal Agripet Vol 13, No 1 (2013): Volume 13, No. 1, April 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i1.554

Abstract

Pathology anatomy changes in liver and bile duct of Aceh cattle infected with fasciola giganticABSTRACT. This study was conducted in Banda Aceh Municipality, Aceh Province to report the pathological changes in liver of Aceh cattle infected with Fasciola gigantica. In this study, livers of male aceh cattles were collected from slaughter area when Qurban in Idul Adha during two years (2011 to 2012). A total of 15 aceh cattle livers were examined. Establishment of F. gigantica were observed, and pathological changes of liver were identified. The result showed that out of 15 male Aceh cattle livers 6 (40%) were found infected with F. gigantica. The common pathological changes found in this study were the hepatic damage, and the bile ducts were changes in size and shape with the dilated lumen. The bile ducts were found filled with blackish brown exudate, which contained adult F. gigantica. In conclusion, fascioliasis is greatly responsible for hepatic damage, and currently expanding as an important veterinary public health problem.
Populasi Ascaridia galli Dalam Usus Halus Ayam Yang Diberikan Kombinasi Ekskretori/Sekretori L3 dan Imunoglobulin Yolk Darmawi Darmawi; Ummu Balqis; Risa Tiuria
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v11i2.370

Abstract

Ascaridia galli populations in intestine of chickens treated with combination of excretory/secretory L3 and immunoglobulin yolkABSTRACT. The purpose of the present study was to determine the presence of worm populations in intestine of chickens vaccinated and combined with egg yolk to experimental Ascaridia galli infection. Amount of 18 head chickens were devided into six groups (A F). Group A, the chickens were not vaccinated. Group B, the chickens were vaccinated with excretory/secretory of A. galli L3. Group C, the chickens were vaccinated with excretory/secretory of A. galli L3, challenged with dose 1000 L2, and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group D, the chickens were vaccinated with excretory/secretory of A. galli L3 and challenged with dose 1000 L2. Group E, the chickens were challenged with dose 1000 L2 and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group E, the chickens were challenged with dose 1000 L2. Intestinal worm burdens of infected groups were recorded. The result showed that excretory/secretory of A. galli L3 combined with egg yolk decreased significantly A. galli survival in intestine of laying hens. Vaccinations were positively correlated with worm burden at 12 weeks after chalanged. The results suggest that A. galli L3 excretory/secretory product contain potential antigen and that antibody-mediated mechanisms contribute to immune protection.
Treatment Of Prolapsus Bulbus Oculi In Domestic Cats Sukma, Zahwa Tamara; Darmawi, Darmawi; Sayuti, Arman; Amiruddin, Amiruddin; Syafruddin, Syafruddin; Daud, Razali; Erwin, Erwin; Panjaitan, Budianto; Ferdian, Riyan; Zamzami, Rumi Sahara; Usrina, Nora; Akmal, Muslim; Balqis, Ummu
Jurnal Medika Veterinaria Vol 19, No 1 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i1.37430

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Bulbus oculi prolapse is a condition characterized by the protrusion of the eyeball from the eye socket, which can be caused by various factors such as trauma, elevated intraocular pressure, and abnormalities in the eye muscles. This case study aimed to examine the treatment of bulbus oculi prolapse in domestic cats. A female calico cat named Mici presented with a chronic left eye injury that had become infected, leading to inflammation and persistent ocular discharge. The cats body weight was 0.7 kg, and its temperature was 38.2C. Surgical treatment was performed using an enucleation technique. The procedure involved an incision along the mid-anterior eyelid, extending to the lower eyelid. Dissection was carried out to expose the bulbus oculi, which was then ligated and excised. The eyelid margins were sutured using simple interrupted sutures. Postoperatively, the cat was administered antibiotics and antihistamines.
Histopathologic Features of Trachea and Lungs in Chickens with Chronic Respiratory Disease Annisa, Aiza; Darmawi, Darmawi; Etriwati, Etriwati; Balqis, Ummu; Salim, M. Nur; Nazaruddin, Nazaruddin; Aliza, Dwinna; Aisyah, Siti; Awaluddin, Awaluddin; Hasan, Denny Irmawati; Akmal, Muslim; Helmi, T. Zahrial; Asmilia, Nuzul
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.37429

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The purpose of this case study is to determine the changes that exist in chickens infected with Chronic Respiratory disease (CRD). A broiler male chicken aged 40 days was found dead in a chicken basket at Ulee Kareng chicken slaughterhouse in a state of feather loss and feces attached to cloaca, on January 6th, 2023. The chicken was brought to pathology laboratory of Faculty of Veterinary Medicine, Universitas Syiah Kuala for necropsy and organ collection. Organs associated with CRD cases were trachea and lungs and histopathological preparations were carried out, stained with Haematoxylin Eosin, and observed under a microscope. The results of histopathological examination showed hemorrhage and hyperplasia of the tracheal epithelium. Hemorrhage was caused by edema which causes increased vascular permeability and epithelial cell hyperplasia occured due to the body's response to infection or inflammation. In the lungs there was bleeding due to damage to the blood vessel wall which caused increased vascular permeability, so that fluid and blood plasma come out of the blood vessels. Fibrinogen then underwent polymerization which resulted in fibrinous thrombi. Congestion happened as the result of fluid accumulation in a location that occured in a passive process so the fluid failed to escape. Because of the accumulation of fluid (congestion), the blood supply to the lung tissue is interrupted, resulting in several points of necrosis in the lungs.
Newcastle Disease Case in Broiler Chicken Angreini, Meliyantika; Balqis, Ummu; Etriwati, Etriwati; Hasan, Denny Irmawati; Aisyah, Siti; Salim, M. Nur; Nazaruddin, Nazaruddin; Darmawi, Darmawi
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.35151

Abstract

Newcastle Disease (ND) is a highly contagious disease with a high mortality rate, infecting poultry, and chickens are very susceptible to ND infection. The purpose of this case report is to find out how to diagnose ND disease in chickens. A 30-day-old white male broiler cadaver obtained from Pasar Lambaro, Aceh Besar was found dead in a holding cage with conjunctival hemorrhage and hair loss. Based on the physical examination of the external condition of the cadaver, it can be seen that the eye membranes are experiencing hemorrhage. There was nasal mucosa hemorrhage and mucous exudate in the nasal cavity. The oral mucosa was also found to have mucous exudate, while the cloaca was bleeding and there was residual green feces. The results of anatomic pathology examination found ptechie in the proventriculus and ventricles, swollen lungs with blackish red color and hemorrhage,with the presence of cheese. There was hemorrhage in other organs including trachea, intestines and tonsils, whilst the brain experienced hyperemia. The spleen was swollen and there were foci of necrosis. Based on the anamnesis, external examination, pathological anatomical examination, and histopathology, the diagnosis of the cause of death of broiler chickens was Newcastle Disease (ND).
EFFECT OF BLACK CUMIN OIL ADMINISTRATION ON CORTISOL LEVEL AND LIVER HISTOPATHOLOGY OF HEAT STRESSED BROILER CHICKENS Hasan, Denny Irmawati; Sugito, Sugito; Sabri, Mustafa; Hambal, Muhammad; Balqis, Ummu
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.12858

Abstract

The aim of this study was to observe cortisol levels and liver histopathology of broiler chicken that were treated with black cumin oil (BCO) under heat stress. A total of 15 broiler chickens were used in this study and divided into 5 groups, K- (without treatment), K+ (given heat stress), P1 (given heat stress and 0.56mL BCO/400kg body weight, P2 (given heat stress and 1.11 mL BCO/400 g body weight), and P3 (given heat stress and 2.22 mL BCO/400 g body weight). Heat stress was given for 5 hours with temperature range of 34-35 C for 7 days. Cortisol was measured using the cortisol enzyme-linked immunosorbent assay (ELISA) kit. Liver histopathology was stained with hematoxylin eosin and observed with electron microscope. The data were analyzed using one way analysis of variance (ANOVA). This study found that application of heat stress to broiler chickens increased cortisol levels and induced histopathological changes in the liver. The BCO administration reduced cortisol level significantly (P0.05) in heat-stressed broilers. BCO administration also significantly reduced (P0.05) the degenerative changes in liver histopathology such as fat degeneration, hemorrhage and necrosis in broiler chickens under heat stress, but did not significantly influence the inflammatory cells infiltration. As conclusion, BCO administration to broiler chickens under heat stress can reduce cortisol levels and minimize histopathological changes in the liver.
Goblet Cells Proliferation of Duodenum, Jejunum, and Ileum of Laying Hens Immunized with Protein of Excretory-Secretory of Ascaridia galli Balqis, Ummu; Tiuria, Risa; Priosoeryanto, Bambang Pontjo; D, Darmawi
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3129

Abstract

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380g of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehosts defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells
Co-Authors . Azhar . Darmawi . Darmawi . Darmawi . Darniati . Fakhrurrazi . Ismail . Muthmainnah . Muttaqien . Nurliana . Rasmaidar Abdul Harris Abdul Harris Abdul Harris Abdul Harris Abdullah Hamzah Abdullah Hamzah Adi Firmansyah Afifah Nur Oriyasmi Aminuddin Aminuddin Amiruddin Amiruddin Amiruddin Amiruddin Ananda Putri Lestari Angreini, Meliyantika Annisa, Aiza Aprilia Wardana Aprilia Wardana Arman Sayuti Arman Sayuti Astri Wulandari Awaluddin Awaluddin Ayu Agita Ginting Azhar Azhar Azhar Azhar Azhar Azhar Azhari Azhari Azmi Zul Baehaqi Bagus Dwijayanti bakti suganjar Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto budianto panjaitan Connie Januari Cut Dahlia Iskandar Cut Mentari Fatihah Amran Cut Nila Thasmi Cut S. Utami Cut Syeila Utami Darmawi . Darmawi D Darmawi D Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Denny Irmawati Hasan Dessy Florenstina BR. G Dian Masyitha Dian Masyitha dian masyithah Didik T Subekti divina dinda hayati Doly Ihsan Siregar Dwina Aliza Dwinna Aliza Dwinna Aliza Dwinna Aliza Eko Karunia Friyan Eliawardani Eliawardani Elsa Mariane Ramadani Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Erina Erina Erwin Erwin Erwin Erwin Erwin Erwin Erwin Erwin Etriwati E EVA FITRIYANINGSIH Eva Fitriyaningsih Fachriyan H Pasaribu Fachriyan H Pasaribu Fachriyan H. Pasaribu Fachriyan H. Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Fadli A Gani Faisal Mustafa Farida Athaillah Farida Athaillah Farida Athaillah Farida Athaillah Febiola Rama Sari Febiola Rama Sari febri ramadana Fera Febrina Ferdian, Riyan Fitriani Fitriani Fitriani Fitriani Fitriani Fitriani Gholib Gholib Ginta Riady Hamdan Hamdan Hamdani Budiman Hamdani Budiman Hamny Sofyan Hasan, Denny Irmawati Hasbalah, Kartini Helmi, T. Zahrial Henni Vanda Hidayati Hidayati Ismail Ismail Ismail Ismail Juli Melia Kulsum Kulsum, Kulsum laksmita patma ningrum M Aman Yaman M Daud AK M Jalaluddin M Nur Salim M Nur Salim M. Hanafiah M. Hasan M. Nur Salim M. Yogi Riyantama Isjoni Mahdi Abrar Marwiyah M Maryam Maryam Maryam Maryam Masda Admi Mirna Safrani Fauzi Muhammad Fathur Ridho Muhammad Hambal Muhammad Hambal Muhammad Hambal Muhammad Hanafiah Muhammad Jalaluddin Muhammad Jalaluddin Mulyadi Adam Mulyadi Mulyadi Muslim Akmal Muslina Muslina, Muslina Mustafa Sabri Muttaqien Bakri Muttaqien Muttaqien Muttaqien Muttaqien N. Nazaruddin NA, Zuhrawaty Nabila Latifa Hafizsha Nabilah Putroe Agung Nanda Afrizan Nauval Gibran Lubis Nazaruddin Nazaruddin Nazaruddin Nazaruddin Nora Usrina Nur rachmatika Nur Salim Nurjannah Annah Nurliana Nurliana Nurul Fitria Nuzul Asmilia Nuzul Asmilia Rahmat Nazif Rasmaidar R Rastina Rastina Raudia Tuzzahra Razali Daud Razali Daud Razali Daud resti aulia putri Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno D. Soejoedono Retno Damayanti Soejoedono Rhoza Indra Rinidar Rinidar Rinidar Rinidar Risa Tiuria rizki nurliyanti siregar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Roza Dea Silvina Rusli Rusli Rusli Rusli Samadi Samadi sariati latif Siti Aisyah Siti Aisyah Siti Aisyah Siti Aisyiah sri yuliati Sugito - Sugito Sugito Sugito Sugito Sukma, Zahwa Tamara Syafruddin Syafruddin Syafruddin Syafruddin Syahrul Syahrul T Armansyah TR T. Armansyah T. Armansyah T. Armansyah TR T. Fadrial Karmil T. Fadrial Karmil T. Reza Ferasyi T. Zahrial Helmi Tapielaniari - Taufik Suryadi, Taufik Teuku R. Ferasyi Triva Murtina Lubis Utami, Cut Suraiya Wahyuni Vinky Claudya Fransiska viola erian Wahyuni, Sri Widya Wati Winaruddin Winaruddin winaruddin winaruddin Yudha Fahrimal Yudha Fahrimal Zainuddin Zainuddin Zainuddin Zainuddin Zamzami, Rumi Sahara zena fisdiora Zico, Hanifa Zuhrawati NA Zuhrawati Zuhrawati Zuraidawati Zuraidawati