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Newcastle Disease Case in Broiler Chicken Angreini, Meliyantika; Balqis, Ummu; Etriwati, Etriwati; Hasan, Denny Irmawati; Aisyah, Siti; Salim, M. Nur; Nazaruddin, Nazaruddin; Darmawi, Darmawi
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.35151

Abstract

Newcastle Disease (ND) is a highly contagious disease with a high mortality rate, infecting poultry, and chickens are very susceptible to ND infection. The purpose of this case report is to find out how to diagnose ND disease in chickens. A 30-day-old white male broiler cadaver obtained from Pasar Lambaro, Aceh Besar was found dead in a holding cage with conjunctival hemorrhage and hair loss. Based on the physical examination of the external condition of the cadaver, it can be seen that the eye membranes are experiencing hemorrhage. There was nasal mucosa hemorrhage and mucous exudate in the nasal cavity. The oral mucosa was also found to have mucous exudate, while the cloaca was bleeding and there was residual green feces. The results of anatomic pathology examination found ptechie in the proventriculus and ventricles, swollen lungs with blackish red color and hemorrhage,with the presence of cheese. There was hemorrhage in other organs including trachea, intestines and tonsils, whilst the brain experienced hyperemia. The spleen was swollen and there were foci of necrosis. Based on the anamnesis, external examination, pathological anatomical examination, and histopathology, the diagnosis of the cause of death of broiler chickens was Newcastle Disease (ND).
EFFECT OF BLACK CUMIN OIL ADMINISTRATION ON CORTISOL LEVEL AND LIVER HISTOPATHOLOGY OF HEAT STRESSED BROILER CHICKENS Hasan, Denny Irmawati; Sugito, Sugito; Sabri, Mustafa; Hambal, Muhammad; Balqis, Ummu
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.12858

Abstract

The aim of this study was to observe cortisol levels and liver histopathology of broiler chicken that were treated with black cumin oil (BCO) under heat stress. A total of 15 broiler chickens were used in this study and divided into 5 groups, K- (without treatment), K+ (given heat stress), P1 (given heat stress and 0.56mL BCO/400kg body weight, P2 (given heat stress and 1.11 mL BCO/400 g body weight), and P3 (given heat stress and 2.22 mL BCO/400 g body weight). Heat stress was given for 5 hours with temperature range of 34-35 C for 7 days. Cortisol was measured using the cortisol enzyme-linked immunosorbent assay (ELISA) kit. Liver histopathology was stained with hematoxylin eosin and observed with electron microscope. The data were analyzed using one way analysis of variance (ANOVA). This study found that application of heat stress to broiler chickens increased cortisol levels and induced histopathological changes in the liver. The BCO administration reduced cortisol level significantly (P0.05) in heat-stressed broilers. BCO administration also significantly reduced (P0.05) the degenerative changes in liver histopathology such as fat degeneration, hemorrhage and necrosis in broiler chickens under heat stress, but did not significantly influence the inflammatory cells infiltration. As conclusion, BCO administration to broiler chickens under heat stress can reduce cortisol levels and minimize histopathological changes in the liver.
Goblet Cells Proliferation of Duodenum, Jejunum, and Ileum of Laying Hens Immunized with Protein of Excretory-Secretory of Ascaridia galli Balqis, Ummu; Tiuria, Risa; Priosoeryanto, Bambang Pontjo; D, Darmawi
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3129

Abstract

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380g of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehosts defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells
L3 Populations in Laying Hens Infected with 6,000 L2 of Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D.; Pasaribu, Fachriyan H.
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3122

Abstract

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae
The Development of Ascaridia galli Infective Eggs by In Vitro Culture Balqis, Ummu; D, Darmawi; Hambal, Muhammad; Tiuria, Risa
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.3104

Abstract

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli, embrionated eggs
KONSENTRASI PROTEIN DAN PENENTUAN BERAT MOLEKUL EKSKRETORI/SEKRETORI L3 Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D; Pasaribu, Fachriyan H
Jurnal Kedokteran Hewan Vol 3, No 1 (2009): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i1.3074

Abstract

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100 ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 510 L3 dikultur secara in vitro dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 g ml-1 streptomisin, 5 g ml-1 gentamisin dan 0,25 g ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.
RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedoeno, Retno Damayanti; Pasaribu, Fachriyan Hasymi; Hambal, Muhammad; Daud, Razali
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.929

Abstract

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 g ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 g ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,631,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,510,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.
Isolation and Identification of Candida sp. in Vagina of Long-Tailed Macaque (Macaca fascicularis) Zico, Hanifa; Erina, Erina; Balqis, Ummu; Rahmi, Erdiansyah; NA, Zuhrawaty
Asian Journal of Social and Humanities Vol. 2 No. 8 (2024): Asian Journal of Social and Humanities
Publisher : Pelopor Publikasi Akademika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59888/ajosh.v2i8.304

Abstract

Candida sp. are commonly found as normal flora in vagina and in certain conditions are opportunistic pathogen. This study aimed to isolation and identification Candida sp. in vagina of the long-tailed macaque (Macaca fascicularis). The sample used in the study was a vaginal swab from five long-tailed macaque (Macaca fascicularis) from Seulawah Forest Aceh Besar. Identification of Candida sp. observed macroscopically by looking at the differences in the color of the colonies grown on the CHROMagar-Candida media. The data obtained were analyzed descriptively. The results of the examination showed that in the vagina of the long-tailed macaque (Macaca fascicularis) there were six species of Candida sp. like C. albicans, C. krusei, C. tropicalis, C. glabrata, C. dubliniensis and C. parapsilosis. It can be concluded that there are several species of Candida sp. which was successfully isolated and identified in vagina of a long-tailed macaque (Macaca fascicularis).
Co-Authors . Azhar . Darmawi . Darmawi . Darmawi . Darniati . Fakhrurrazi . Ismail . Muthmainnah . Muttaqien . Nurliana . Rasmaidar Abdul Harris Abdul Harris Abdul Harris Abdul Harris Abdullah Hamzah Abdullah Hamzah Adi Firmansyah Afifah Nur Oriyasmi Aminuddin Aminuddin Amiruddin Amiruddin Amiruddin Amiruddin Ananda Putri Lestari Angreini, Meliyantika Annisa, Aiza Aprilia Wardana Aprilia Wardana Arman Sayuti Arman Sayuti Astri Wulandari Awaluddin Awaluddin Ayu Agita Ginting Azhar Azhar Azhar Azhar Azhar Azhar Azhari Azhari Azmi Zul Baehaqi Bagus Dwijayanti bakti suganjar Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto budianto panjaitan Connie Januari Cut Dahlia Iskandar Cut Mentari Fatihah Amran Cut Nila Thasmi Cut S. Utami Cut Syeila Utami Darmawi . Darmawi D Darmawi D Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Denny Irmawati Hasan Dessy Florenstina BR. G Dian Masyitha Dian Masyitha dian masyithah Didik T Subekti divina dinda hayati Doly Ihsan Siregar Dwina Aliza Dwinna Aliza Dwinna Aliza Dwinna Aliza Eko Karunia Friyan Eliawardani Eliawardani Elsa Mariane Ramadani Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Erina Erina Erwin Erwin Erwin Erwin Erwin Erwin Erwin Erwin Etriwati E Eva Fitriyaningsih EVA FITRIYANINGSIH Fachriyan H Pasaribu Fachriyan H Pasaribu Fachriyan H. Pasaribu Fachriyan H. Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Fadli A Gani Faisal Mustafa Farida Athaillah Farida Athaillah Farida Athaillah Farida Athaillah Febiola Rama Sari Febiola Rama Sari febri ramadana Fera Febrina Ferdian, Riyan Fitriani Fitriani Fitriani Fitriani Fitriani Fitriani Gholib Gholib Ginta Riady Hamdan Hamdan Hamdani Budiman Hamdani Budiman Hamny Sofyan Hasan, Denny Irmawati Hasan, Desi Salwani Hasbalah, Kartini Helmi, T. Zahrial Henni Vanda Hidayati Hidayati Ismail Ismail Ismail Ismail Juli Melia Kulsum Kulsum, Kulsum laksmita patma ningrum M Aman Yaman M Daud AK M Jalaluddin M Nur Salim M Nur Salim M. Hanafiah M. Hasan M. Nur Salim M. Yogi Riyantama Isjoni Mahdi Abrar Maimun Syukri, Maimun Marwiyah M Maryam Maryam Maryam Maryam Masda Admi Mirna Safrani Fauzi Muhammad Fathur Ridho Muhammad Hambal Muhammad Hambal Muhammad Hambal Muhammad Hanafiah Muhammad Jalaluddin Muhammad Jalaluddin Mulyadi Adam Mulyadi Mulyadi Muslim Akmal Muslina Muslina, Muslina Mustafa Sabri Muttaqien Bakri Muttaqien Muttaqien Muttaqien Muttaqien N. Nazaruddin NA, Zuhrawaty Nabila Latifa Hafizsha Nabilah Putroe Agung Nanda Afrizan Nauval Gibran Lubis Nazaruddin Nazaruddin Nazaruddin Nazaruddin Nora Usrina Nur rachmatika Nur Salim Nurjannah Annah Nurliana Nurliana Nurul Fitria Nuzul Asmilia Nuzul Asmilia Rachmad Suhanda Rahmat Nazif Rasmaidar R Rastina Rastina Raudia Tuzzahra Razali Daud Razali Daud Razali Daud resti aulia putri Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno D. Soejoedono Retno Damayanti Soejoedono Rhoza Indra Rinidar Rinidar Rinidar Rinidar Risa Tiuria rizki nurliyanti siregar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Roza Dea Silvina Rusli Rusli Rusli Rusli Samadi Samadi sariati latif Siti Aisyah Siti Aisyah Siti Aisyah Siti Aisyiah sri yuliati Sugito - Sugito Sugito Sugito Sugito Sukma, Zahwa Tamara Syafruddin Syafruddin Syafruddin Syafruddin Syahrul Syahrul T Armansyah TR T. Armansyah T. Armansyah T. Armansyah TR T. Fadrial Karmil T. Fadrial Karmil T. Reza Ferasyi T. Zahrial Helmi Tapielaniari - Taufik Suryadi, Taufik Teuku R. Ferasyi Triva Murtina Lubis Utami, Cut Suraiya Wahyuni Vinky Claudya Fransiska viola erian Wahyuni, Sri Widya Wati Winaruddin Winaruddin winaruddin winaruddin Yudha Fahrimal Yudha Fahrimal Zainuddin Zainuddin Zainuddin Zainuddin Zamzami, Rumi Sahara zena fisdiora Zico, Hanifa Zuhrawati NA Zuhrawati Zuhrawati Zuraidawati Zuraidawati