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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Agromet Jurnal Ilmu dan Teknologi Kelautan Tropis Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Widyariset KOVALEN: Jurnal Riset Kimia Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Jurnal Intek Akuakultur Kelola: Journal of Islamic Education Management Indonesian Aquaculture Journal Jurnal Riset Akuakultur Journal of Educational Research and Evaluation BJRA (Bongaya Journal of Research in Accounting) Efektor JSMA (Jurnal Sains Manajemen dan Akuntansi) Widyariset Jurnal Aktiva : Riset Akutansi dan Keuangan Qadauna: Jurnal Ilmiah Mahasiswa Hukum Keluarga Islam Journal of Applied Civil Engineering and Infrastructure Technology (JACEIT) Jumper : Jurnal Mahasiswa Pendidikan Olahraga Jurnal Media Akuakultur Indonesia Shautuna: Jurnal Ilmiah Mahasiswa Perbandingan Mazhab Jurnal Pengabdian Inovasi dan Teknologi Kepada Masyarakat SinarFe7 Private Law Journal of Applied Sciences in Accounting, Finance, and Tax Jurnal Pengabdian Masyarakat Olahraga dan Kesehatan Vokatek : Jurnal Pengabdian Masyarakat PEDAMATH: Journal on Pedagogical Mathematics Al Fuadiy: Jurnal Hukum Keluarga Islam Economos : Jurnal Ekonomi dan Bisnis Journal Sultra Research of Law ENLEKTURER: Journal of Islamic Studies HISABUNA: Jurnal Ilmu Falak Inovasi Teknologi Masyarakat Journal of Mathematics, Computation and Statistics (JMATHCOS) International Journal of Education, Vocational and Social Science Hikamatzu Journal of Multidisciplinary Research Bersama: Jurnal Pengabdian Kepada Masyarakat Issues in Mathematics Educations (IMED) At-Tadris: Journal of Islamic Education J-Sil (Jurnal Teknik Sipil dan Lingkungan)

Alimuddin

Departemen Budidaya Perairan, Fakultas Perikanan Dan Kelautan, Institut Pertanian Bogor Jl. Agatis, Kampus IPB Dramaga 16680

Author-ID : 2471926

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Evaluation of different promoters driving the GFP reporter gene in seaweed Kappaphycus alvarezii Muh. Alias L. Rajamuddin; A. Alimuddin; Utut Widyastuti; Irvan Faizal
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Promoter regulates expression level of foreign gene in transgenic organism. This study was performed to select asuitable promoter as the fi rst step towards production of valuable trait-enhanced seaweed by transgenic technology. Greenfl uorescent protein (GFP) gene was used as a reporter to determine the activity of promoter in seaweed Kappaphycusalvarezii. GFP gene constructs driven by cytomegalovirus (pCMV-GFP), caulifl ower mosaic virus (pCaMV-GFP),medaka β-actin (pmBA-GFP) and Japanese fl ounder keratin (pJfKer-GFP) promoters were introduced by electroporationmethod. Electroporation was performed using a gene pulser (BIORAD) with voltage of 300 V, pulse length of 0.5 ms,pulse numbers of 4, and pulse interval of 0.1 s. Promoter activity was determined by analyzing GFP gene expressionlevel using a fl uorescent microscope. The results showed that CMV regulated highest number of fi lament callus(34.10%±1.49) expressing GFP at medium to strong fl uorescence levels. CaMV promoter had relatively similar activitywith CMV, but lower number of fi lament callus expressing GFP (10.48%±0.25). mBA promoter drove GFP expressionat medium level and similar number of fi lament callus (8.85%±2.31) expressing GFP with CaMV, while JfKer promoterhad lowest activity by means in number of fi lament callus expressing GFP (4.79%±0.26) and GFP expression level. PCRanalysis for transgenic confi rmation showed a DNA band of PCR product from pCMV-GFP and pCaMV-GFP expressingfi lament callus in the same size (about 0.6 kb) with positive control of plasmid. Thus, CMV and CaMV promoters wasan appropriate promoter and foreign gene could be transferred to fi lament callus by electroporation method. Combiningthis achievement with developing a culture method of fi lament callus to be thallus, stable transgenic breeding in K.alvarezii can be feasible.

GROWTH IN NURSERY AND GROW-OUT PHASES OF WHITE SHRIMP AFTER IMMERSED IN RECOMBINANT GIANT GROUPER GROWTH HORMONE Aulia Saputra; Odang Carman; . Alimuddin
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 7 No. 2 (2015): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

The growth of white shrimp (Litopenaeus vannamei) can be improved by using recombinant fish growth hormone through immersion. This research was performed to evaluate the white shrimp growth at nursery and grow-out phases after recombinant giant grouper growth hormone (rElGH) immersion. Shrimp were immersed at different stages in one liter seawater containing 15 mg rElGH for two hours. At the nursery stage 30,000 PL4 that previously immersed at nauplius stage (treatment A1), at PL4 (treatment B1) and control without rElGH immersion (K1) were reared in fiber tanks containing 750 L seawater for 8 days. At the grow-out phase, 100 PL11 that have been immersed in rElGH solution at nauplius stage (treatment A2), at PL4 (B2), PL11 (C2) and control without rElGH immersion (K2) were separately reared in fiber tanks containing 750 L seawater for 55 days. Each treatment consisted of three replications. The results showed that at the end of the nursery phase, B1 treatment increased 19% of body length, 30.2% of the body weight and decreased the coefficient of length variation 34.9% compared to control K1 (P<0.05). At the grow-out phase, C2 treatment enhanced 38.2% of body weight and 32% of biomass compared with control K2 (P<0.05). Thus, hatchery is better to immerse PL4, and the farmer should used rElGH-immersed PL11 for growing-out.Keywords: growth promoting, nursery, grow out, Litopenaeus vannamei, rElGH

IMPROVEMENT METHOD OF GENE TRANSFER IN Kappaphycus alvarezii St. Hidayah Triana; . Alimuddin; Utut Widyastuti; . Suharsono; Emma Suryati; Andi Parenrengi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Method of foreign gene transfer in red seaweed Kappaphycus alvarezii has been reported, however, li-mited number of transgenic F0 (broodstock) was obtained. This study was conducted to improve the method of gene transfer mediated by Agrobacterium tumefaciens in order to obtain high percentage of K. alvarezii transgenic. Superoxide dismutase gene from Melastoma malabatrichum (MmCu/Zn-SOD) was used as model towards increasing adaptability of K. alvarezii to environmental stress. The treat-ments were the culture media and recovery duration, and each treatment consisted of three replica-tions. The best method was co-cultivation using liquid media, then recovery was conducted in liquid media for 10 days. That treatment allowed higher transformation percentage (90%), regeneration effi-ciency (90%), putative bud efficiency (100%), number of buds and explants sprouted (100%) and transgenic explants (100%). The transgenic explants showed an amplification PCR product of Mm-Cu/Zn-SOD gene fragment, whereas the non-transgenic explants showed no amplification product. All results revealed that suitable method of transgenesis for K. alvarezii has been developed. Keywords: Agrobacterium tumefaciens, culture media, Kappaphycus alvarezii, recovery duration, transformation

BINARY VECTOR CONSTRUCTION OF KAPPA(κ)-CARRAGEENASE GENE AND TRANSFORMATION TO Agrobacterium tumefaciens AS MEDIATOR FOR SEAWEED TRANSGENIC GENERATION Muh Alias L. Rajamuddin; . Alimuddin; Utut Widyastuti; Enang Harris; Emma Suryati
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Increasing of kappa (κ)-carrageenan content in Kappaphycus alvarezii seaweed is potentially be achieved by applying transgenesis technology. This study was performed to obtain a construction of κ-Carrageenase gene and Agrobacterium tumefaciens to carry those construction genes. The κ-Carrageenase (κ-Car) gene was involved in κ-carrageenan biosynthesis. The κ-Car gene sequence was ligated between the 35S CaMV promoter and tNos terminator sequences to generate pMSH/κ-Car expression vector. Transformation of pMSH/κ-Car plasmid to Escherichia coli was performed by heat-shock method, and to Agrobacterium tumefaciens by tri-parental mating method. The results showed that several colonies of E. coli and A. tumefaciens grew in the selective culture mediums containing antibiotic. PCR analysis using primers 35S-Forward and tNos-Reverse with DNA template from those bacterial colonies resulted DNA fragment of about 2,000 bp, the same as the total length of 35S CaMV promoter, κ-Car gene and tNos terminator sequences. Therefore, the construction of pMSH/κ-Car gene was succeeded and a colony of A. tumefaciens transformant carrying pMSH/κ-Car plasmid was successfully produced. Keywords: Agrobacterium tumefaciens, kappa(κ)-Carrageenase gene, transgenesis, vector

PENGARUH PEMBERIAN HORMON 17β-ESTRADIOL TERHADAP PERKEMBANGAN GONAD SIPUT GONGGONG Laevistrombus turturella Muzahar; Muhammad Zairin Jr.; Fredinan Yulianda; Muhammad Agus Suprayudi; Alimuddin; Irzal Effendi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 11 No. 3 (2019): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Dog conch Laevistrombus turturella is captured intensively, therefore, their population is decline. The dog conch culture is needed to maintain their population. The technology towards conch reproduction is still not developed yet in Indonesia. The 17β-estradiol hormone in conch and some mollusks has been stated by some researcher yet its main role in conch reproduction process has not widely known. The study about the addition of 17β-estradiol hormone in accelerating gonad development has never been reported. The aim of this study was to evaluate the impact of the addition of 17β-estradiol against dog conch’s gonad development. This study used three treatments for three groups of dog conch those were without injection (P1), injection by 30 µL of corn oil mixed with absolute ethanol (P2), and 30 µL of 17β-estradiol stock solution (P3). After injection, the dog conch was reared in pens culture in their natural habitat for 30 days. This study showed that the injection of a 17β-estradiol solution (P3) stimulated the dog conch’s gonad development as evidenced by greater oocyte mean diameter than another treatment. The mean of gonadal weight and GSI on P3 treatment was also higher than treatments P1 and P2. SDS-PAGE analysis showed that dog conch’s hemolymph has several kinds of proteins with varying molecular weights. Proteins with a molecular weight of 54-55 kDa are predicted as dog conch’s vitellogenin.

SELEKSI INDUK MATANG GONAD PADA SIPUT GONGGONG Laevistrombus turturella Muzahar; Muhammad Zairin Jr.; Fredinan Yulianda; Muhammad Agus Suprayudi; Alimuddin; Irzal Effendi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 12 No. 1 (2020): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Siput gonggong Laevistrombus turturella merupakan siput laut yang banyak dikonsumsi masyarakat di Provinsi Kepulauan Riau sehingga populasinya diperkirakan menurun. Upaya pelestarian siput ini perlu dilakukan salah satunya dengan budidaya. Kegiatan budidaya memerlukan ketersediaan induk matang gonad. Tujuan penelitian ini adalah menganalisis ukuran induk siput gonggong matang gonad berdasarkan panjang cangkang dan warna gonad, serta mengevaluasi pengaruh paparan suhu berbeda terhadap kecepatan penentuan jenis kelamin induk. Panjang cangkang siput gonggong diukur dengan jangka sorong, kemudian dipecahkan untuk mengamati warna gonadnya. Data dianalisis dengan student t-test. Perlakuan paparan suhu air 15°C (P1), 20°C (P2), dan 30°C (P3). Tujuh ekor induk siput gonggong dimasukkan ke dalam akuarium sesuai perlakuan, durasi waktu keluar tubuh lunak siput gonggong dari cangkang dicatat. Data dianalisis dengan uji Kruskal Wallis. Hasil penelitian menunjukkan ukuran rata-rata panjang cangkang induk siput gonggong matang gonad dari laut Madong-Tanjungpinang adalah 63,449±5,352 mm (jantan) dan 66,953±5,876 mm (betina). Penentuan jenis kelamin siput gonggong dapat dipercepat dengan pemberian kejutan suhu dengan suhu air 20°C.

TRANSPLANTATION OF GIANT GOURAMY (Osphronemus gouramy) TESTICULAR CELLS IN EARLY HATCHING TILAPIA (Oreochromis niloticus) LARVAE Jasmadi Jasmadi; Odang Carman; Alimuddin Alimuddin
Widyariset Vol 2, No 2 (2016): Widyariset
Publisher : Pusbindiklat - LIPI

Cell transplantation technology has been widely developed in mammals, but less implemented in inland aquaculture fish. Giant gouramy (Osphronemus gouramy) is one of important comercial aquaculture commodities that has a relatively long first reproductive cycle compared to the tilapia (Oreochromis niloticus). Tilapia has a faster reproductive cycle and potential as giant gouramy surrogate broodstock. This study was aimed to applicate testicular cell transplantation technology of giant gouramy in tilapia larvae and to evaluate its success rate trough donor cell colonization. Tilapia larvae of 1-2 dph (days post hatching) and 3-4 dph were injected with ±20.000 giant gouramy testicular cells in the peritoneal cavity, and then groomed for two months. Cell colonization of spermatogonium donor was determined by Polymerase Chain Reaction (PCR), using giant gouramy F1-Growth Hormone and R1-Growth Hormone primers. The result of cell dissociation showed that a bigger giant gouramy (827 g) had lower of spermatogonia composition (4.45%) than a smaller gouramy (608 g) (14.96%). Donor cell colonization in 1-2 dph recipient was higher (100%) than in 3-4 dph recipient (75%). Whereas recipient Survival Rate (SR) was 89.34% for 1-2 dph recipient and 98.96% for 3-4 dph recipient respectively. This study suggests that testicular cell transplantation technology of giant gouramy could be appllied to tilapia (Xenotransplantation) and cell transplantation has a better performance in 1-2 dph than in 3-4 dph recipients. With such success, there is a high potential that the giant gouramy cultivation can be increased through the cell transplantation technology.

cDNA ENCODING GROWTH HORMONE FROM HUMPBACK GROUPER (Cromileptes altivelis) Mochamad Syaifudin; Alimuddin; Utut Widyastuti; Agus Oman Sudrajat; Komar Sumantadinata; Ratu Siti Aliah
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 14 No. 2 (2007)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2007.14.2.14

Growth hormone (GH) that plays an important role in growth, reproduction, seawater adaptation, and immune function was isolated and sequenced from humpback grouper, Cromileptes altivelis. The cDNA was isolated from pituitary using RT-PCR. The 618 bp open reading frame encodes a 205 amino acid (aa) protein, which represents an 18 aa signal peptide followed by a 187 aa mature GH polypeptide. The fragment contained conserved domain of somatotropinâ€“1, somatotropinâ€“2, casein kinase II phosphorylation, protein kinase C phosphorylation, Nâ€“myristoylation and Nâ€“glycosilation. The similarity of deduced protein of humpback grouper GH was 65.0 - 89.5% with other fishes

COMPARISON OF THREE DIFFERENT TECHNIQUES OF GENE TRANSFER IN HUMPBACK GROUPER ( ) CROMILEPTES ALTIVELIS KOMAR SUMANTADINATA; SLAMET SUBYAKTO; ALIMUDDIN; RUSTIDJA; M. SASMITO JATI; IRVAN FAIZAL; RATU SITI ALIAH; GEMI TRIASTUTIK
BIOTROPIA Vol. 18 No. 1 (2011)
Publisher : SEAMEO BIOTROP

Humpback grouper is one of the most cultured fishes in Asia, including Indonesia. Themain problemfaced by humpback culture is its slow growth rate.One of themethods that willbe more effective and efficient to solve the problem is using transgenic technique. This studywas conducted to determine the effectiveness of transfection,microinjection and electroporationtechniques on gene transfer in humpback grouper. transfection was performed byincubating sperm to the foreign DNA (pktBP-ktGH gene construct)-transfectant complexsolution, while was by injecting those complex solution into testis of mature males.Microinjection was conducted in 2-4 cell stage embryos using 25 Î¼g/ml of foreign DNAsolution, and duration of injection was 1, 2 and 3 seconds. Electroporation by 50 V, 30 ms ofpulse length, 5 of pulse number and 0.1 of pulse interval was performed to sperm using threeDNA concentration of 5, 10 and 20 Î¼g/ml. The incorporation of foreign DNA in sperm andembryos were analyzed using PCR method. Based on PCR analysis, an optimum DNAconcentration for electroporation was 10 Î¼g/ml. Limited number of embryos could bemicroinjected during 20-30 min to reach 2-4 cell stage. Microinjection for 1 second showedhigher survival rate of embryos, although none or very low number of larvae was hatched.Transfast was an effective DNA delivery reagent for humpback grouper sperm. Foreign DNAcould be detected in sperm from two out of ten transfected fish at least 36 hours posttransfection (hpt). By transfection, foreign DNA was detected in sperm at 48 hpt 25 Cincubation temperature. Our study revealed that transfection, microinjection as well aselectroporation could be used as transgenesis methods in humpback grouper. By means ofsimplicity and efficacy, however, electroporationwas an appropriate gene transfermethod.oIn vitroin vivoin vivoin vitro

GROWTH AND MEAT QUALITY ENHANCEMENT OF STRIPED CATFISH (Pangasianodon hypophthalmus) USING RECOMBINANT GROWTH HORMONE Reni Agustina Lubis; Alimuddin Alimuddin; Nur Bambang Priyo Utomo
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 1 (2019)
Publisher : SEAMEO BIOTROP

The application of fish recombinant growth hormone (rGH) has been known as one of the methods to improve the growth performance of cultured fishes, one of which is the striped catfish Pangasianodon hypophthalmus, a species that is becoming commercially attractive in Indonesia. Hence, this study was aimed to evaluate the effects of rGH supplementation in commercial diet on the growth, feed utilization and flesh quality in P. hypophthalmus grow out. The rGH was mixed with chicken egg yolk and sprayed on the commercial feeds with different protein levels (32, 28 and 23%). In the control, the feeds were also sprayed with chicken egg yolk but without rGH.Â Striped catfish with body weights of 110.66 Â± 1.32 g ind-1 were fed on rGH-supplemented diets two times a week during the first and third months, and during the rest of the months they were fed on diet without rGH supplementation. The fishes were reared for 120 days in 18 hapa (2Ã—1Ã—1.5 m3) with initial density of 20 fishes per hapa. The result showed that the highest weight gain, specific growth rate (SGR), and lowest feed conversion ratio (FCR) were obtained by fishes fed on 32% protein content with rGH-supplemented diet. No significant difference was observed in the weight gain, SGR and FCR in rGH treated group with 28% protein content with rGH supplemented diet and non-rGH control group receiving 32% protein diet. Similar moisture content of meat, protein content of meat, belly fat and edible portion were observed in rGH-supplemented diet and their control. Except in the treatment 23% protein content rGH supplemented diet that has lower lipid content in fish body and meat. The highest SGR was obtained when the fishes were fed on the 32% protein feed combined with rGH. Enrichment with rGH depleted the fat content in the meat of fish fed on all levels of protein in which the lowest fat was found in the 23% protein feed.

Co-Authors . Irmawati . Kurdianto . Suharsono A. Octavera A. Santika Abdul Halim Talli Abyadul Fitriyah Ade Sunarma Adi Sucipto, Adi Adinda Rizky Tasrianty Afdal Madein Afif Abdurrahman Afpriyaningrum MD Agus Oman Sudrajat Agus Priyadi Ahmad Faisal Ahmad Muzaki Ahmad Ramzi Ghulam Syam Ahmad Zaki Alfiah Mutmainnah Ali Alyaminy, Humaidatul Aminah Zaim Aman Sentosa Panggabean Amin, Muhmmadiyah Andi Aliah Hidayani Andi Haerul Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi, K. Petta Lolo, Arjuni Andi, Maulana K. Angela Mariana Lusiastuti Anggriani, Emi Anhar ANNA OCTAVERA Anny Hary Ayu Apriwanto, Indra Aras Syazili Arief Boediono Arief Eko Prasetiyo Arien Heryansyah Asdar Asep Permana Asep Sopian Asri Ainun Amaliah Aulia Saputra Awi Dassa Ayi Santika Azis Ali Baderiah Baderiah Bahri, Nur Alim Benyamin Timotiwu, Paul Bohari Yusuf Boyun Handoyo Budimawan Chaeroni, Ahmad Chaerunnisa Chotban, Sippah DARMAWAN SETIA BUDI Darussalam Darwin, Khadijah DEDI JUSADI Delfian Masrura Deny Sapto Chondro Utomo Desy Nurul Astuti Devi Wardan Sari Dewi Yuniati Dian Hardiantho Dian Hardiantho Diki Nurbaldatun Islam Dinamella Wahjuningrum Dinar Tri Soelistyowati Djadir Dwi Hany Yanti Eko Pramono Emma Suryati Emma Suryati Enang Harris Enang Harris Endi Permata Eni Kusrini Eni Kusrini Erma Primanita Hayuningtyas F.H. Pasaribu Fachruddin Fachruddin Fadhila Maharani Putri Fajar Anggraeni Fatihah, Anariski Fatimah Akbal Fauzan Wahib Alsani Fauziah Zainuddin Firman Firmansyah Fitriadi Fitriyah Husnul Khotimah Fitry Hasdanita Fredinan Yulianda G. Yoshizaki GEMI TRIASTUTIK Gloria Ika Satriani Goro Yoshizaki GORO YOSHIZAKI Gunawan Gunawan Gustikasari Amin H. Arfah Hadi Nasbey Hamda Hamid Habbe Hamzah Hanipa Hardiantho D Harmayani, Ria Harton Arfah Hary Krettiawan Haryanti Haryanti Hasan Nasrullah Hassan, Muhammad Nasiru Herjayanto, Muh. Hisyam Ihsan Ibnu Izzah Idrus Sahar Muhammad Iis Diatin Ika Kartika Ikhwanul Arifan ILHAM Ilham Minggi Imron Imron Indah Mastuti Irma Andriani IRMA ANDRIYANI Irmawati, Budimawan IRVAN FAIZAL Irvan Faizal Irvan Faizal Irwan Irzal Effendi Ita Djuwita Jasmadi Jasmadi Jean-Christophe Avarre Joko Santoso Jubaedah Julie Ekasari Jumrah123 K. Chaitanya Deepti K. Sumantadinata Kadriani, Nur Annisa Kamaruddin Kartini, Selvira Febriani Katarina Manik, Tumiar Ketut Mahardika Ketut Sugama Khaerullah khairuzzazi KOMAR SUMANTADINATA Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Leman, Swanggi Lianasari, Ika Yekti Lidyasari Liza Luky Adrianto M. SASMITO JATI M. Zairin Junior M.Y. Jamal Madein, Afdal Maharani, Adinda Marwah, Andi Marwah Mashud MEDIATY Megawati, Novi Mia Setiawati Miftahul Jannah Mira Mawardi MMSI Irfan ,S. Kom Mochamad Syaifudin Muh Alias L. Rajamuddin Muh. Akmal, Andi Muh. Alias L. Rajamuddin MUHAMMAD AGUS SUPRAYUDI Muhammad Ammar Naufal Muhammad Awal Ramadhan Muhammad Firdaus Muhammad Ikhsan Muhammad Jufri Muhammad Muttaqin Muhammad Sabir Muhammad Safir Muhammad Wawan Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammadiyah Amin MUNTI YUHANA Muslim Muslim Mustika Sari, Mustika Musyfikah Ilyas Muzahar N.A. Maswan Nadhirah Nagu Nagu, Nadhirah Nasrullah Nenima Halawa Nining Asniar Ridzal Noerlitasari Nugraha, Adrianto Fahrizal Nunak Nafiqoh Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur, Rezki Amaliah Nurdin Arsyad Nurfadilla Nurhalija Nurhayati Nurhidayat, Muhammad Nurlaelah Nurlaelah Syarifuddin Nurly Faridah Nurly Faridah Nurul Fadhilah Nurwati Djam’an O. Carman O.Z. Arifin Odang Carman Oman Unju Subandi P. Ratna Kumar Permata Sari, Anggun Petrus Rani Pong-Masak Priatama, Rizki Purwaningsih, Yuli Pustika Ratnawati Putri, Angelina Atika R Rifai R.D. Soejoedono Rahmi, Kurnia Anggraini RATU SITI ALIAH Ratu Siti Aliah Ratu Siti Aliah Reni Agustina Lubis Ridwan RIDWAN AFFANDI Risfiyanty, Dwi Kartika Riswandi Rizky Ayu Fatimah Ridwan Rodliyah Rohayu, Rina Rosidah Rusli RUSTIDJA S. Mariam Samsu Adi Rahman Sani, NurHaslina Sanusi Saputra, Ade Maulana Sari , Nur Indah Sari Budi Moria Sembiring Sarmila Sekar Sulistyaning Hadiwibowo Setiawati M Shabarullah Shalsyabila , Zabina Shandi Irawan Sholeh Ridwan Siti Aslamyah Siti Fadilah Sitti Fakhriyyah SLAMET SUBYAKTO Soelistyowati DT Soerja Koesnarpadi Soko Nuswantoro Sri Khayati sri murtini . Sri Nuryati Sri Nuryati Srihartini, Yusi St. Fatmawati. L St. Hidayah Triana St. Mukarramah Sukenda . Sukenda Sukenda Sukmawati Ali Sumardin Raupu Sundari Sutamrin Syafrianto, Syafrianto Syuhada Restu Danupratama T. Takeuchi Tahrim, Tasdin Talib, Dr. Ahmad Tasdin Tahrim Tedy Murtedjo Tri Setyo Guntoro Usman Mulbar Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Wa Ode NurRadhiah Ridjalu Wahidah Sanusi Wartono Hadie Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Y. Hadiroseyani Yulintine Yulintine Yuni Puji Hastuti Yuningsih, Andri Yusra, Andi Zairin Jr M Zakki Zainun

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