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EFFECTS OF DIETARY RECOMBINANT GROWTH HORMONE WITH WATERBORNE CALCIUM CARBONATE SUPPLEMENTATION ON GROWTH PERFORMANCE AND GH–IGF AXIS-RELATED GENE EXPRESSION IN JUVENILE SNAKEHEAD (Channa striata) Indah Permata Sari; Alimuddin Alimuddin; Agus Oman Sudrajat; Tatag Budiardi
Indonesian Aquaculture Journal Vol 21, No 1 (2026): (June, 2026)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.21.1.2026.23-33

Abstract

This study evaluated the effects of dietary recombinant growth hormone (rGH) and waterborne calcium carbonate (CaCO3) supplementation on growth performance, feed utilization, body composition, and growth-related gene expression in snakehead (Channa striata) juveniles. The experiment was conducted for 90 days using four treatments: control (R0C0, without rGH and CaCO3 supplementation), treatment 1 (R5C0, 5 mg kg-1 rGH and without CaCO3  supplementation), treatment 2 (R5C25, rGH and 25 mg L-1 CaCO3 supplementation), and treatment 3 (R5C50 rGH and 50 mg L-1 CaCO3  supplementation), each with six replicates. The results showed that rGH significantly increased final body weight (BWt) and specific growth rate, and reduced feed conversion ratio (FCR) compared with the control. Final body weight was significantly higher in R5C0 and R5C50 than in the control, with no significant difference between these treatments. In contrast, body length increased significantly with increasing CaCO3  dose, with the highest value observed in R5C50. Feed conversion ratio decreased significantly in all rGH treatments but did not differ among R5C0, R5C25, and R5C50. Protein and lipid retention were highest in R5C0 and decreased with CaCO3  supplementation. Body calcium content increased with CaCO3  supplementation, with the highest value observed in R5C50. Gene expression analysis showed that rGH significantly increased GH and IGF-1 expression compared to the control, while no consistent significant differences were observed among combined treatments. These findings suggest that rGH predominantly enhanced weight gain and feed utilization, whereas CaCO3  supplementation under rGH treatment was associated with increased body length. The combined treatments resulted in complementary but parameter-specific effects.
ESTIMATION OF HERITABILITY AND SELECTION RESPONSE FOR BODY LENGTH OF NEON TETRA (Paracheirodon innesi) FROM BOJONGSARI DISTRICT, INDONESIA Siti Zuhriyyah Musthofa; Alimuddin Alimuddin; Harton Arfah; Dinar Tri Soelistyowati; Odang Carman; Eni Kusrini
Indonesian Aquaculture Journal Vol 21, No 1 (2026): (June, 2026)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.21.1.2026.1-11

Abstract

The neon tetra (Paracheirodon innesi), a popular South American ornamental fish, has become a key species in Indonesian aquaculture, with production centered in Bojongsari, Depok. However, the industry faces declining genetic quality due to inbreeding. Therefore, implementing a selective breeding program is considered an effective approach to improve genetic quality. This study aimed to estimate the heritability and selection response of the body length trait in the base population of neon tetra fish. Selection was conducted on the entire population using a within-family selection method, specifically employing broodstock from the Curug and Bojongsari populations. A reciprocal breeding design was established using four mating combinations, each comprising 25 pairs of 4- to 5-month-old broodstock (3.28±0.15 cm total length; 0.43±0.06 g body weight) mated at a 1:1 sex ratio. The offspring were reared separately and grown out in an aquarium for 120 days. The results showed that the estimated heritability was 0.42, with a response to selection of 0.13 cm (4.9%). Our finding of moderate heritability implies that this trait can be effectively enhanced through a systematic breeding program. These figures suggest that the selection process for the next generation of neon tetra fish remains viable.
Evaluation of Genetic Variation in Hybrid Catfish (Sangkuriang × Thai) Employing PCR-RAPD Markers Kurnia Anggraini Rahmi; Putriku Nadzira Sativa; Alimuddin Alimuddin; Dinar Tri Soelistyowati
Jurnal Perikanan UGM (Journal of Fisheries Sciences) Vol 28, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.113208

Abstract

Hybridization is the process of crossing individuals with different genotypes to produce offspring with superior traits. In this study, Sangkuriang catfish were crossed with introduced Thai catfish to obtain heterosis in hybrid seeds. The objective was to analyze the genetic diversity of these hybrid seeds using the PCR-RAPD method. Specifically, the primers OPA-13, OPB-08, OPC-19, and OPD-02 were used. Crossbreeding involved Sangkuriang female and Thai male broodstock, with four pairs of broodfish used via a semi-natural spawning method. After spawning, hybrid seeds from each pair were reared separately in containers under uniform conditions for 1.5 months. DNA samples were then collected from the fin tissue of each replicate of the crosses, and were grouped according to size categories: large (7-8 cm), medium (5-6 cm), small (3-4 cm), and broodstock, with 30 samples per size category for each replicate. The results revealed a polymorphism percentage of 72.95-93.88%, heterozygosity values of 0.167-0.232, and an average genetic distance among hybrid seeds from the three size categories and broodstock ranging from 0.001-0.018.
cDNA ENCODING GROWTH HORMONE FROM HUMPBACK GROUPER (Cromileptes altivelis) Mochamad Syaifudin; Alimuddin; Utut Widyastuti; Agus Oman Sudrajat; Komar Sumantadinata; Ratu Siti Aliah
BIOTROPIA Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2007.14.2.14

Abstract

Growth hormone (GH) that plays an important role in growth, reproduction, seawater adaptation, and immune function was isolated and sequenced from humpback grouper, Cromileptes altivelis. The cDNA was isolated from pituitary using RT-PCR. The 618 bp open reading frame encodes a 205 amino acid (aa) protein, which represents an 18 aa signal peptide followed by a 187 aa mature GH polypeptide. The fragment contained conserved domain of somatotropin–1, somatotropin–2, casein kinase II phosphorylation, protein kinase C phosphorylation, N–myristoylation and N–glycosilation. The similarity of deduced protein of humpback grouper GH was 65.0 - 89.5% with other fishes
COMPARISON OF THREE DIFFERENT TECHNIQUES OF GENE TRANSFER IN HUMPBACK GROUPER ( ) CROMILEPTES ALTIVELIS KOMAR SUMANTADINATA; SLAMET SUBYAKTO; ALIMUDDIN; RUSTIDJA; M. SASMITO JATI; IRVAN FAIZAL; RATU SITI ALIAH; GEMI TRIASTUTIK
BIOTROPIA Vol. 18 No. 1 (2011): BIOTROPIA Vol. 18 No. 1 June 2011
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.406 KB) | DOI: 10.11598/btb.2011.18.1.134

Abstract

Humpback grouper is one of the most cultured fishes in Asia, including Indonesia. Themain problemfaced by humpback culture is its slow growth rate.One of themethods that willbe more effective and efficient to solve the problem is using transgenic technique. This studywas conducted to determine the effectiveness of transfection,microinjection and electroporationtechniques on gene transfer in humpback grouper. transfection was performed byincubating sperm to the foreign DNA (pktBP-ktGH gene construct)-transfectant complexsolution, while was by injecting those complex solution into testis of mature males.Microinjection was conducted in 2-4 cell stage embryos using 25 μg/ml of foreign DNAsolution, and duration of injection was 1, 2 and 3 seconds. Electroporation by 50 V, 30 ms ofpulse length, 5 of pulse number and 0.1 of pulse interval was performed to sperm using threeDNA concentration of 5, 10 and 20 μg/ml. The incorporation of foreign DNA in sperm andembryos were analyzed using PCR method. Based on PCR analysis, an optimum DNAconcentration for electroporation was 10 μg/ml. Limited number of embryos could bemicroinjected during 20-30 min to reach 2-4 cell stage. Microinjection for 1 second showedhigher survival rate of embryos, although none or very low number of larvae was hatched.Transfast was an effective DNA delivery reagent for humpback grouper sperm. Foreign DNAcould be detected in sperm from two out of ten transfected fish at least 36 hours posttransfection (hpt). By transfection, foreign DNA was detected in sperm at 48 hpt 25 Cincubation temperature. Our study revealed that transfection, microinjection as well aselectroporation could be used as transgenesis methods in humpback grouper. By means ofsimplicity and efficacy, however, electroporationwas an appropriate gene transfermethod.oIn vitroin vivoin vivoin vitro
GROWTH AND MEAT QUALITY ENHANCEMENT OF STRIPED CATFISH (Pangasianodon hypophthalmus) USING RECOMBINANT GROWTH HORMONE Reni Agustina Lubis; Alimuddin Alimuddin; Nur Bambang Priyo Utomo
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.589 KB) | DOI: 10.11598/btb.2019.26.1.710

Abstract

The application of fish recombinant growth hormone (rGH) has been known as one of the methods to improve the growth performance of cultured fishes, one of which is the striped catfish Pangasianodon hypophthalmus, a species that is becoming commercially attractive in Indonesia. Hence, this study was aimed to evaluate the effects of rGH supplementation in commercial diet on the growth, feed utilization and flesh quality in P. hypophthalmus grow out. The rGH was mixed with chicken egg yolk and sprayed on the commercial feeds with different protein levels (32, 28 and 23%). In the control, the feeds were also sprayed with chicken egg yolk but without rGH.  Striped catfish with body weights of 110.66 ± 1.32 g ind-1 were fed on rGH-supplemented diets two times a week during the first and third months, and during the rest of the months they were fed on diet without rGH supplementation. The fishes were reared for 120 days in 18 hapa (2×1×1.5 m3) with initial density of 20 fishes per hapa. The result showed that the highest weight gain, specific growth rate (SGR), and lowest feed conversion ratio (FCR) were obtained by fishes fed on 32% protein content with rGH-supplemented diet. No significant difference was observed in the weight gain, SGR and FCR in rGH treated group with 28% protein content with rGH supplemented diet and non-rGH control group receiving 32% protein diet. Similar moisture content of meat, protein content of meat, belly fat and edible portion were observed in rGH-supplemented diet and their control. Except in the treatment 23% protein content rGH supplemented diet that has lower lipid content in fish body and meat. The highest SGR was obtained when the fishes were fed on the 32% protein feed combined with rGH. Enrichment with rGH depleted the fat content in the meat of fish fed on all levels of protein in which the lowest fat was found in the 23% protein feed.
RESISTANCE AGAINST Aeromonas hydrophila INFECTION AND GROWTH OF SECOND GENERATION (F2) AFRICAN CATFISH (Clarias gariepinus) USING SELECTED MOLECULAR MARKERS Alimuddin Alimuddin; Fadhila Maharani Putri; Dinamella Wahjuningrum; Dian Hardiantho; Ade Sunarma; Sri Nuryati
BIOTROPIA Vol. 25 No. 2 (2018): BIOTROPIA Vol. 25 No. 2 August 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1246.846 KB) | DOI: 10.11598/btb.2018.25.2.742

Abstract

Aeromonas hydrophila is a pathogenic bacteria that causes mass mortality in catfish. In previous studies, specific pathogen resistant (SPR), A. hydrophila-resistant African catfish first generation (F1) has been cultivated by marker assisted selection using the major histocompatibility complex (MHC) I as a molecular marker. In this study, growth performance, inheritance of the MHC DNA marker in the second generation (F2) of catfish and disease resistance against A. hydrophila infection were observed. The F2 progenies were produced by crossing F1 fish between themselves. Nursery was performed in 80-L glass aquaria, 4 replications for each cross, at the same initial density, for 2 months of rearing. The results showed that daily growth rate of F2 progenies from the SPR broods was significantly higher than those from broods without the marker. Results of the PCR analysis showed that average number of F2 progenies from SPR broods carrying the MHC marker was about 91% higher than that of control. After the fish reached about 12 cm body length, they were challenged by intramuscularly injecting of 0.1 mL A. hydrophila (LD₅₀: 10⁵ CFU/mL) for 7 days. Results of challenge test showed that survival of F2 offspring from the crosses of SPR broods (77.2%) was about two times higher than those from brood without MHC marker (38.3%). Differential leukocyte count supported the high resistance of F2 progenies from F1 broods having MHC I marker against A. hydrophila infection. In conclusion, African catfish farming carrying MHC marker potentially have higher productivity and reduces fish loss due to infection by A. hydrophila.
GROWTH PERFORMANCE AND ENZYME ACTIVITIES IN CATFISH (Pangasianodon hypophthalmus) FED WITH WATER HYACINTH-BASED DIET Dewi Yuniati; Nur Bambang Priyo Utomo; Mia Setiawati; Alimuddin Alimuddin
BIOTROPIA Vol. 25 No. 2 (2018): BIOTROPIA Vol. 25 No. 2 August 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.2.840

Abstract

An alternative substitution of pollard as an imported feed ingredient is a necessity, and one of the potential ingredients is water hyacinth (Eichhornia crassipes). This study was conducted to evaluate growth performance and enzyme activity in catfish (Pangasianodon hypophthalmus). Diet with five different levels of water hyacinth substitute of pollard (0%, 25%, 50%, 75%, and 100%) were fed to catfish for 60 days. Seventy (70) catfish fry with average initial body weight of 2.45 ± 0.15 g were maintained in 100 cm × 80 cm × 60 cm aquaria. Fish fed at satiation level three times daily at 8 am, 12 pm, and 4 pm. With dietary 25% water hyacinth, growth performance and protease activity similar to 0% treatment. Feed intake, protein digestibility, feed efficiency, protein efficiency ratio, and protease and amylase enzyme activities decreased (p<0.05) in those fed with more than 25% water hyacinth. Catfish fed 25% water hyacinth showed significantly (p<0.05) higher daily growth rate, feed efficiency and protein digestibility than those with other treatments. Based on the growth performance and enzyme activity, we can conclude that the optimum dietary level of water hyacinth substitute pollard for fry catfish is 25%.
Dynamics of fish diversity across an environmental gradient in the Seribu Islands reefs off Jakarta HAWIS H. MADDUPPA; BEGINER SUBHAN; ENY SUPARYANI; ACHIS M. SIREGAR; DONDY ARAFAT; SUKMARAHARJA A. TARIGAN; ALIMUDDIN ALIMUDDIN; DENNY KHAIRUDI; FADHILLAH RAHMAWATI; ADITYA BRAHMANDITO
Biodiversitas Journal of Biological Diversity Vol. 14 No. 1 (2013)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d140103

Abstract

Madduppa HH, Subhan B, Suparyani E, Siregar AM, Arafat D, Tarigan SA, Alimuddin, Khairudi D, Rahmawati F, Bramandito A. 2013.Â&nbsp;Dynamics of fish diversity across an environmental gradient in the Seribu Islands reefs off Jakarta. Biodiversitas 14: 17-24. The reefs ofÂ&nbsp;Seribu Islands have been affected by multitude of anthropogenic pressures. However, the biodiversity of reef fishes across theÂ&nbsp;archipelago linked to environmental condition is poorly known. This study aimed to investigate the biodiversity and the trophic level ofÂ&nbsp;fish communities across the archipelago. The study on reef fish communities was conducted on 33 reef sites associated with islands orÂ&nbsp;shoal randomly chosen from each zone along environmental gradients from the inshore water nearest of Jakarta Bay to the offshoreÂ&nbsp;water of the outer islands. The study sites represented each sub-districts within the archipelago, namely Pari, Tidung, Panggang, Kelapa,Â&nbsp;and Harapan. A total of 46,263 individual fishes were counted, belonging to 216 species and 29 families. The multivariate analysis ofÂ&nbsp;fish abundance using the Bray Curtis similarity index and non-metric multidimensional scaling (MDS) clearly showed the clustering ofÂ&nbsp;sub-districts, near and far from Jakarta Bay. The results showed that the sub-districts can be clustered into three groups. Group oneÂ&nbsp;consists of one sub-district (Pari) located in the southern part of the Seribu Islands near Jakarta Bay. Group two consists of three subdistricts (Tidung, Panggang, Kelapa)Â&nbsp;located in mid of the archipelago. The third group consists of one sub-district (Harapan) located inÂ&nbsp;the northern part of the Seribu Islands. Based on species richness and fish diversity indices, the sub-districts can be clustered into twoÂ&nbsp;groups (1 = Pari and Tidung, 2 = Panggang Kelapa, Harapan). However, levels of similarities among sub-districts varied. The fishÂ&nbsp;community in sub-district of Pari was dominated by carnivorous, omnivorous and herbivorous fishes, while those in the rest of subdistricts were dominated by omnivorous and carnivorous fishes. The present study results showed that the biodiversity of reef fishesÂ&nbsp;across the Seribu Islands seemed to be linked to the environmental conditions.
Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus Hasan Nasrullah; Yanti Inneke Nababan; Ika Safitri; Dwi Yanti; Sri Nuryati; Muhammad Zairin Jr; Alimuddin Alimuddin
Biodiversitas Journal of Biological Diversity Vol. 21 No. 1 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210138

Abstract

Abstract. Authors. 2020. Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus. Biodiversitas 21: 311-317. The chicken-type lysozyme (LYSC) gene has been demonstrated to play important roles in the fish protection system against bacterial infection. In this present study, we aimed to identify the single nucleotide polymorphism (SNP) within the LYSC gene of African catfish Clarias gariepinus and its possible association with Aeromonas hydrophila resistance, a major pathogenic bacterium in African catfish. The gDNA of the African catfish LYSC coding sequence was 1559 bp long, comprising of four exons and three introns. Six SNPs were identified in African catfish LYSC, namely SNP1-6. After the A. hydrophila challenge, we regarded the surviving individuals after the infection as the resistant group and the dead fish as the susceptible group. High-resolution melting (HRM) analysis on SNP2 revealed that the allele frequencies of TT, CC, and TC were of 27.78%, 5.55%, and 66.67% in the resistant group, while the frequencies of TT, CC, and TC were 16.67%, 27.78%, and 55.55% in the susceptible group, respectively. The expression of LYSC and other immune-genes in the resistant group was also higher in the liver, head kidney, and spleen. These results indicated that the LYSC gene might play an essential role in bacterial resistance, and the SNP2 within the LYSC gene may be associated with the resistance to A. hydrophila in African catfish.
Co-Authors . Irmawati . Kurdianto . Suharsono A. Octavera A. Santika Abinawanto ACHIS M. SIREGAR Achmad Sudradjat Ade Sunarma ADITYA BRAHMANDITO Afif Abdurrahman Afpriyaningrum MD AGUS O. SUDRAJAT Agus Oman Sudrajat Agus Priyadi Agus Priyadi Agus Priyadi Ahmad Muzaki AKHMAD TAUFIQ MUKTI Amrullah Amrullah Anang Hari Kristanto Andi Aliah Hidayani Andi Aliah Hidayani Andi Aliah Hidayani Andi Haerul Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi Tenriulo Andi Tenriulo Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANNA OCTAVERA Anny Hary Ayu Aras Syazili Arief Boediono Arief Boediono Arief Eko Prasetiyo Aris Tri Wahyudi Asep Permana Asep Permana Asep Sopian Asep Sopian ASMI CITRA MALINA A. R. TASSAKKA Aulia Saputra Aulia Saputra Ayi Santika Ayi Santika Ayi Santika Azis Azis Bagus Rahmat Basuki Bambang Iswanto Beginer Subhan Boyun Handoyo Boyun Handoyo Budimawan DARMAWAN SETIA BUDI Darussalam DEDI JUSADI DENNY KHAIRUDI Deny Sapto Chondro Utomo Desy Nurul Astuti Dewi Yuniati Dian Hardiantho Dian Hardiantho Dian Novita Sari Dian R Herdianto Dinamella Wahjuningrum Dinar Tri Soelistiyowati Dinar Tri Soelistyowati Dinar Tri Sulistyowati Dinar Tri Sulistyowati Dody Dharmawan Trijuno Dody Dharmawan Trijuno Dondy Arafat Dwi Hany Yanti Dwi Hany Yanti Dwi Yanti Emma Suryati Emma Suryati Enang Harris Enang Harris Enang Harris Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eny Heriyati ENY SUPARYANI Erma Primanita Hayuningtyas Erma Primanita Hayuningtyas Estu Nugroho Evi Tahapari F.H. Pasaribu Fadhila Maharani Putri FADHILLAH RAHMAWATI Fajar Anggraeni Fajar Maulana Fauzan Wahib Alsani Fitriyah Husnul Khotimah Fredinan Yulianda G. Yoshizaki GEMI TRIASTUTIK Gloria Ika Satriani Goro Yoshizaki Goro Yoshizaki Goro Yoshizaki GORO YOSHIZAKI Gunarto Gunarto Gusrina Gusrina H. Arfah Hafidah, Riva Hardiantho D Harton Arfah Hary Krettiawan Haryanti Haryanti HASAN NASRULLAH Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah HAWIS H. MADDUPPA Herjayanto, Muh. Huria Marnis Huria Marnis I.W. Teguh Wibawan Iis Diatin Ika Safitri Ikhsan Khasani Imron Imron Imron Imron INCE AYU KHAIRANA KADRIAH Indah Mastuti Indah Permata Sari Indra Lesmana Intan Febrianti Irin Iriana Kusmini Irma Andriani Irma Andriani IRMA ANDRIYANI Irmawati IRMAWATI IRMAWATI Irmawati, Budimawan Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal IRVAN FAIZAL Irvan Faizal Irvan Faizal Irzal Effendi Ita Djuwita Ita Djuwita Jasmadi Jasmadi Jean-Christophe Avarre Joko Santoso Joni Haryadi Jr., Muhammad Zairin Julie Ekasari K. Chaitanya Deepti K. Sumantadinata Kamaruddin Ketut Mahardika Ketut Sugama khairuzzazi Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata KOMAR SUMANTADINATA Kukuh Nirmala Kurnia Anggraini Rahmi Lies Emmawati Hadie Luky Adrianto M. SASMITO JATI M. Zairin Junior M.Y. Jamal Maharani, Adinda Maskur Maskur Megawati, Novi MEIMULYA MEIMULYA Melta Rini Fahmi Mia Setiawati Mira Mawardi Mochamad Syaifudin Moh. Tauhid Umar Mohammad Zairin Mohammad Zairin Mubinun Mubinun Muh Alias L. Rajamuddin Muh. Alias L. Rajamuddin MUHAMMAD AGUS SUPRAYUDI Muhammad Firdaus Muhammad Hunaina Fariduddin Ath-thar Muhammad Hunaina Fariduddin Ath-thar Muhammad Husni Amarullah Muhammad Muhammad Muhammad Muttaqin Muhammad Safir Muhammad Yamin Muhammad Zairin Jr Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. MUHAMMAD ZAIRIN JR. Mulyasari Mulyasari MUNTI YUHANA Muslim Muslim Muzahar N.A. Maswan Nadia Ayuningthias NADIARTI NADIARTI NITA RUKMINASARI Nita Rukminasari Nita Rukminasari Novi Megawati Novi Megawati Nunak Nafiqoh Nunuk Listiyowati Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo NURBETY TARIGAN Nurly Faridah Nurly Faridah O. Carman O.Z. Arifin Odang Carman P. Ratna Kumar Petrus Rani Pong-Masak Priadi Setyawan Pustika Ratnawati Putriku Nadzira Sativa R Rifai R.D. Soejoedono Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi Ratu Siti Aliah Ratu Siti Aliah RATU SITI ALIAH Ratu Siti Aliah Rendy Ginanjar Reni Agustina Lubis Ridwan RIDWAN AFFANDI Rommy Suprapto Ruby Vidia Kusumah Rudhy Gustiano RUSTIDJA S. Mariam Samsu Adi Rahman Sari Budi Moria Sembiring Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Setiawati M Shabarullah Siska Aliyas Sandra Siti Aslamyah Siti Fadilah Siti Khodijah Siti Subaidah Siti Zuhriyyah Musthofa Sitti Fakhriyyah SLAMET SUBYAKTO Soelistyowati DT Soko Nuswantoro Sri Murtini Sri Nuryati Sri Nuryati St. Hidayah Triana Suci Antoro Sukenda, Sukenda, SUKMARAHARJA A. TARIGAN Sularto Sularto Sularto Sularto Sutanti Sutanti Syuhada Restu Danupratama T. Takeuchi Tatag Budiardi Toshio Takeuchi Tristiana Yuniarti Uswatun Hasanah Uswatun Hasanah Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Wartono Hadie Wartono Hadie Wartono Hadie Wartono Hadie Wartono Hadie Wedaraningtyas Nugrahani Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wiwien Mukti Andriyani Wiwien Mukti Andriyani Y. Hadiroseyani Yanti Inneke Nababan yogi Himawan Yulintine Yulintine Yuni Puji Hastuti Yushinta Fujaya Yushinta Fujaya Zairin Jr M Zakki Zainun