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Antibacterial Activity of Ethyl Acetate and Cream Formulation of Coleus atropurpureus leaves Against Staphylococcus aureus Tarigan, Indra Lasmana; Muadifah, Afidatul; Susanto, Nindita Clourisa Clourisa Amaris; Huda, Choirul
Pharmaceutical Journal of Indonesia Vol. 7 No. 1 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2021.007.01.1

Abstract

Infectious diseases caused by Staphylococcus aureus (Sa) bacteria reaching 70% of cases in Asia and can attack and survive in epithelial cells including endothelial cells. Attempts to overcome the infection caused by Sa by giving antibiotics, which work as antibacterial. Myana (Coleus atropurpureus L. Benth) leaves contain several active compounds that might act as antibacterial agents. This study aimed to analyze the antibacterial activity of Coleus Extract in vitro and to evaluate the formulation of the extract cream against the bacteria causing infection, Sa. Coleus leaves ethyl acetate extract contains alkaloids and tannins, the absorbance of Sa at the 3rd hour was getting smaller, from 0.668 to 0.552, this showed that the longer the incubation time of the extract against bacterial inoculation, the more bacteria died. Our cream formulation with criteria: the pH value, homogeneity, spreadability, adhesion, and protection power. Our cream formulation results were then evaluated based on the standard, with criteria: the pH value, homogeneity, spreadability, adhesion, and protection power. It seems that our cream is per the cream standards. It is semi-solid, brownish-white in color, smells typical of Coleus, and pH 6. Moreover, the cream is homogeneous with 5 cm of spreadability, 7 seconds for adhesion, and colorless. Moreover, we found that Coleus extract cream has better antibacterial activity than positive control with a wound closure time of 7-14 days for extract while more than 14 days for positive control.
Profile Analysis of Fatty Acids of Tengkawang (Shorea Sumatrana) Oil Using GC-MS and Antibacterial Activity Riski, Diah Gusti; Maulana, Rico Gewana Resdy; Permana, Edwin; Lestari, Intan; Tarigan, Indra Lasmana
Indonesian Journal of Chemical Research Vol 8 No 2 (2020): Edition for September
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2020.8-dgr

Abstract

The Shorea sumatrana (tengkawang) plant is endemic to Indonesia, especially in Kalimantan and Sumatera regions, which produces chemical diversity especially as a natural drugs. Specific aims to investigate both the profile analysis of fatty acid and antibacterial potential of tengkawang oil. The extract of tengkawang oil was carried out using the soxhlet extraction method. The profile analysis of fatty acid was verified by GC-MS and the antibacterial activity was evaluated using disc-diffusion method. The profile analysis of fatty acid of tengkawang oil indicated the presence of palmitic acid (17.26%), stearic acid (60.68%), oleic acid (11.98%), oleic acid chloride (1.80%), stearic acid chloride (1.86%), glycidyl stearate (1.92%), diethyl phthalate (4%), and 2-monopalmitin (0.5%). We determined the antibacterial activity by the diameter of inhibition of growth zone against Salmonella enteritidis, Escherichia coli, Staphylococcus aureus, and Bacillus cereus at a concentration of 12.5%, 25%, 50%. These were compared with standard tetracycline as positive control and DMSO was assigned negative control. It was found that the highest percentage of fatty acid in tengkawang oil is stearic acid, at 60.68%, and that tengkawang oil is an antibacterial agent with a concentration optimum at 25% with more susceptibility to Gram-positive than Gram-negative bacteria.
Pertumbuhan Bibit Kopi Liberika Tungkal Komposit pada Aplikasi Pupuk Kandang Ayam Lizawati, Lizawati; Kartika, Elis; Latief, Madyawati; Tarigan, Indra Lasmana; Sutrisno, Sutrisno; Puspitasari, Ratih Dyah
Jurnal Agroekoteknologi Vol 16, No 1 (2024)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v16i1.22467

Abstract

One of the efforts to increase the growth of Liberica coffee of Tungkal composite seedlings is through organic fertilization as considered inorganic fertilizers replacement. The purpose of this study was to look for the best dose of chicken manure for the growth of Liberica coffee of Tungkal composite seedlings. This study used a randomized block design with 4 replications with one factor, namely chicken manure consisting of 7 treatment levels, namely 0, 50, 100, 150, 200, 250 and 300 g of chicken manure/polybag. The variables observed were increase in seedling height, increase in stem diameter, increase in number of leaves, leaf area, crown dry weight, and root dry weight. The results showed that the application of chicken manure was able to increase the increase in seedling height, increase in stem diameter, increase in the number of leaves, and crown dry weight of liberica coffee seedlings, but was not able to increase leaf area and root dry weight. The recommended dose of chicken manure to increase the growth of liberica coffee seedlings was 211 g of chicken manure/polybag.
Production Process Technology of Lecithin from Crude Palm Oil on The Physico Chemical Properties of Halal Dark Chocolate produced Latief, Madyawati; Nizori, Addion; Rahmi, Silvi Leila; Nabila, Annisa Candra; Tarigan, Indra Lasmana; Huda, Nurul; Yusof, Yus Aniza
Indonesian Food Science and Technology Journal Vol. 6 No. 2 (2023): Volume 6 Number 2, July 2023 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v6i2.29184

Abstract

This research was conducted to determine the effect of the addition of CPO (Crude Palm Oil) lecithin as an Emulsifier on the physicochemical and sensory characteristics of dark chocolate, and to determine the amount of lecithin addition as an appropriate Emulsifier to produce chocolate with the best physico-chemical and sensory characteristics. This study used a completely randomized design (CRD) method with a treatment level of 0%. 0.1%, 0.2%, 0.3%, 0.4% with 4 repetitions to obtain 20 experimental units. Parameters observed included color degee, antioxidant activity, fat blooming, stability level, color sensory test, taste, aroma, texture and overall acceptability. The data obtained were analyzed using ANOVA levels of 1% and 5%. If there is a significant effect on the treatment, it will be continued with the Duncan's Multiple Range Test (DNMRT) at the 5% level. The best concentration of adding lecithin to dark chocolate is 0.3% which contains 66.76% antioxidant activity, color degee L*28.58, a* 13.67, b* 22.67, hue 58.75, good stability (melting properties), able to prevent fat blooming, has good color very chocolatey (4.20), very distinctive aroma of chocolate (4.44), bitter taste (2.68), smooth texture (3.36) and the overall acceptance of the panelists really liked it (8.16).
Encapsulation of Ethanol Extract Perepat Leaves (Sonneratia alba) with Maltodextrin Coating as an Antioxidant Functional Food Candidate Heriyanti, Heriyanti; Putri, Yunika Eka; Irawan, Hengky; Raza'i, Tengku Said; Tarigan, Indra Lasmana; Kartika, Elis; Ichwan, Budiyati; Latief, Madyawati
Indonesian Food Science and Technology Journal Vol. 7 No. 2 (2024): Volume 7 Number 2, July 2024 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v7i2.34013

Abstract

Pemanfaatan Limbah Batang Pisang Dan Hama Keong Mas Sebagai Pakan Alternatif Pada Ternak Unggas Wahyuni, Linda; Khasanah, Misbahul; Jamilah, Jamilah; Lestari, Suci; Suci, Rasyifa; Fikram, Muhammad; Tusa’diah, Halimah; Ikhwal, Muhamad; Saputra, Ardiyanto; Prayoga, Adi; Tarigan, Indra Lasmana
Jurnal Pengabdian Masyarakat Pinang Masak Vol. 3 No. 2 (2022): Desember 2022
Publisher : Fakultas Sains dan Teknologi, Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jpm.v3i2.21429

Abstract

Batang pisang merupakan salah satu limbah yang tidak dimanfaatkan oleh masyarakat dan keong mas merupakan salah satu hama paling merugikan bagi petani, terutama pada awal masa tanam. Kedua bahan tersebut memiliki potensi yang baik untuk dimanfaatkan sebagai olahan pakan ternak unggas. Proses pengolahan pakan ternak ini melalui proses fermentasi yang dibantu dengan EM4 dan molase sebagai activator dan sumber nutrisi bagi bakteri. Bahan yang digunakan dalam penelitian ini adalah batang pisang, keong mas, EM4 peternakan, molase, dan garam. Sedangkan alat yang digunakan adalah mesin pencacah batang pisang, mesin penggiling keong mas, dan mesin penepung. Proses pengolahan pakan ternak unggas ini dimulai dengan mencacah batang pisang sampai halus kemudian di fermentasi beberapa hari dan dijemur hingga kering. Keong dipisahkan antara daging dengan cangkangnya kemudian di haluskan, lalu dijemur sampai kering. Kemudian batang pisang dan keong yang halus di masukan ke mesin penepung agar benar benar halus selanjutnya, dicampur rata. Pakan ternak unggas ini mengandung serat kasar %, kadar air %, protein %, abu %, dan lemak %. Pakan yang baik memiliki bau asam segar, warna kuning kecoklatan, tekstur lembut serta mudah dipisahkan. Berdasarkan Penelititan yang dilakukan, dihasilkan luaran berupa dedak pakan ternak unggas dengan bahan baku batang pisang dan keong mas
Phytochemistry and Antidiabetic Evaluation in-vitro and in-vivo of Ethyl Acetate Fractions from Sungkai Leaves (Peronema canescens Jack) Aini, Qurrota; Latief, Madyawati; Tarigan, Indra Lasmana; MASRURI, Masruri
The Journal of Pure and Applied Chemistry Research Vol. 13 No. 2 (2024): Edition May-August 2024
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2024.013.02.700

Abstract

The paper reports the in vivo and in vitro antidiabetic activity of bioactive compounds composed of ethyl acetate fractions from Sungkai (Peronema canescens Jack). The fractions are obtained from vacuum liquid chromatography (F1, F2 and F3). Phytochemicals screening, UV-Vis and FTIR spectrometry analysis is applied to detect secondary metabolite composed of the fractions. In addition, in vitro antidiabetic capability is measured inhibiting a-amylase activity, and in vivo evaluation is conducted using diabetic rat's model induced by alloxan. It was found that fraction collected as a white precipitate. The phytochemical analysis indicated positive for flavonoid. UV-Vis and FTIR spectra support the data. Moreover, capability of the fractions to inhibit a-glucosidase activity in the range 5-100 ppm does not show the activity. Conversely, the in vivo evaluation from fraction 2 at concentration 600 mg/Kg of body weight has antidiabetic in 50.16%. This result comparable to that of glibenclamide as a control. This finding lead to further evaluation for structural elucidation.
In Silico Study of Bioactive Compounds of Putat Leaf Extract (Planchonia valida) as Anti-Cancer Against the VEGFR2 Receptor Bela, Anisa; Farid, Faizar; Nelson; Dewi, Tribuana Tungga; Prabawa, Satria; Khatib, Alfi; Yusnaidar, Yusnaidar; Latief, Madyawati; Tarigan, Indra Lasmana
Journal of The Indonesian Society of Integrated Chemistry Vol. 16 No. 1 (2024): Journal of The Indonesian Society of Integrated Chemistry
Publisher : Pendidikan Kimia FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jisic.v16i1.32049

Abstract

Cancer is the process of forming new tissue that is abnormal and malignant. Efforts to discover anti-cancer drug compounds continue to be made to minimize the toxic effects of chemotherapy, so it is necessary to look for other alternatives to treat cancer. This research aims to determine the anti-cancer activity of putat plant bioactive compounds through identification of receptor targets and interaction studies using the molecular docking method against the VEGFR2 (Vascular endothelial growth factor receptor-2) receptor. The results showed that in the test of eight putat plant bioactive compounds the best results were obtained for the compound 3-oxo-N-(1,3-thiazol-2-yl) butanamide with a binding free energy value of -9.86 kcal-mol-1 with an inhibition constant value of 1.47 Micromolar. The best docking result has an inhibition constant value that is higher than the native ligand and has a binding free energy that is almost close to the binding free energy of the native ligand with an inhibition constant value of 42.38 Micromolar and a binding free energy value of 10.06 kcal-mol-1. Therefore, the results of the docking of the bioactive compound 3-oxo-N-(1,3-thiazol-2-yl) butanamide with the VEGFR-2 receptor are considered capable of being an alternative as a candidate for anti-cancer drugs.
Enhancement of Liberica Coffee Quality by Wet Fermentation using Bacillus subtilis Indra Lasmana Tarigan; Ericha Aulia; Heriyanti Heriyanti; Madyawati Latief; Sutrisno Sutrisno
ALCHEMY Jurnal Penelitian Kimia Vol 20, No 2 (2024): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.20.2.74248.162-177

Abstract

Coffee is a tree species in the Rubiaceae family and Coffea genus. One way to enhance the value of coffee commodities is by using fermentation technology. Fermentation can use probiotic bacteria found in mongoose digestive tracts, creating coffee with a unique taste and aroma. Bacillus subtilis is one of these bacteria, offering an alternative to those from mongoose. This study aimed to analyze the effects of fermentation using B. subtilis bacteria on the physicochemical and sensory properties of Liberica coffee. The study method included bacteria preparation, coffee fermentation, and testing. The samples' total phenolics, flavonoids, caffeine, and chlorogenic acid contents were estimated. The analysis was performed using UV-Vis and GC-MS spectrophotometers. The results revealed that the fermentation process using B. subtilis improved the sensory and physical qualities of the fermented Liberica coffee. The total values of phenolic and flavonoid contents and antioxidants of the fermented Liberica coffee were higher than that of the original coffee. GC-MS analysis exhibited compounds in the fermented coffee in which the highest was N-Dodecyl-N-(trifluoroacetyl)dodecanamide (C26H48F3NO2), with a retention time of 23.73 minutes and an area percentage of 18.96%. Moreover, the caffeine and chlorogenic acid of the fermented Liberica coffee was lower than that of the original coffee.
LC-MS Based Metabolite Profiling of Ethanol Extract From the Sungkai (Peronema canescens Jack) and In Silico Prediction of Antidiabetic Activity With α-Glucosidase Latief, Madyawati; Arum, Dewi; Nurjannah, Sofia; Tarigan, Indra Lasmana; Khatib, Alfi; Yusnaidar, Yusnaidar
Molekul Vol 19 No 3 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.3.8806

Abstract

Sungkai (Peronema canescens Jack) is a medicinal plant in the Verbenaceae family that is known to have antidiabetic activity. The secondary metabolite content found in sungkai leaves is in the form of alkaloids, flavonoids, triterpenoids, steroids, phenolics, and saponins. This research aimed to identify the biological activity of compounds in sungkai plants as antidiabetic agents using α-glucosidase inhibitors in silico. Sungkai leaf extract with ethanol solvent was identified using a Liquid Chromatography Mass Spectrometer (LC-MS/MS). There are 15 compounds resulting from LC-MS/MS analysis which will then predict antidiabetic activity using molecular docking. Molecular docking was carried out using AutoDock Tools software and visualized with Discovery Studio. The highest scoring result obtained from the molecular docking test was the compound C13H23N6S with a free bond energy result of -7.31 and an inhibition constant value of 4.41 μM, which binds three hydrogen bonds in GLU 78, ALA 75, and GLU 198. Keywords: In sillico, metabolite profilling, Peronema canescens
Co-Authors Addion Nizori Ade Lestari Adi Prayoga, Adi Adriliana, Zairaini Adriliana, Zairaini Adriliana Afidatul Muadifah Afidatul Muadifah Agustin, Putri Ahmad Fadhil Ahmad Sazali aini, annisa nurul Alfi Khatib, Alfi Ali Nurdin Hidayat Ali, Noha Amanda, Hilda Ananda, Hertanti Dwi Andini, Paziati Anggi Ayunda Triani Anggi Ayunda Triani Anggi Cahaya Nirwana Anggun Tri Fisesa Anisa Anisa Ardian Salsa Rusmana Arsa, Daniel Arum, Dewi Arum, Kartika Ayu Natalia Azira, Ayu Azizah, Regina Wan Bagus, Adhitya Eko Bambang Hariyadi Bela, Anisa Betharia Juli Mas Tambunan Betri Yanda Boy Candra Sitanggang Br Sembiring, Vera Lorensya Budiyanto Budiyanto Budiyati Ichwan Bulan, Catherine Putri Cintya, Henni Delviani, Delviani Denny Prabawa, Aditya Devi Safitri Dewi Arum Dewi Natalia R Dewi Susanti Dewi, Aisha Andini Indira Dewi, Tribuana Tungga Dhian Eka Wijaya Diah Riski Gusti Dian Natasya Dyah Puspitasari, Ratih Edwin Permana Edwin Permana Elis Kartika Ely Sulistiara Emi Lestari Ericha Aulia Etonihu, Anayo Christian Excel Emerlan Samiar Wasistha Faizar Farid Farah, Farah Fauzan Azima Fendri Lukmanto Fikram, Muhammad Fikriansyah, Muhammad Fiolita Etsa Aurora Fisesa, Anggun Tri FITRY TAFZI Hamdan Maruli Siregar Handaru, Windu Helda Wika Amini Hengky Irawan, Hengky Heriyanti Heriyanti Heriyanti Heriyanti, Heriyanti Heriyanti, Septina Is Hertanti Dwi Ananda Hertanti Hertanti Hertanti, Hertanti Hilda Amanda Humaryanto, Humaryanto Husna, Naimul Hutapea, Jobin Armando Ihsan, Mahya Ika Ani Ikhwal, Muhamad Intan Lestari Intan Lestari Jamaludin Al-Anshori Jamilah Jamilah Jovanncha, Czellicya Kartika Arum Wardani Kharisma, Tiara Khasanah, Misbahul Khusna, Mursyidah Lathifatul Lenny Marlinda Lizawati Lizawati Lumbantoruan, Ricardo M Ikrar Lagowa Madyawati Latief Madyawati Latief Madyawati Latief Madyawati Latief Madyawati Latief Madyawati Latief Maharani, Rizky Maharani, Vioala Giary Rizkillah Maharani, Viola Giary Rizkillah Marbun, Sarah Aknesia Marsella Oktavioni Marudut Sinaga Masruri Masruri Maulana, Rico Gewana Resdy Melani, Fitria Metik Ambarwati Moh Nabawi Muadifah, Afidatul Muhaimin Muhaimin Muhaimin Muhaimin Muhaimin Muhammad Iqbal Muhammad Iqbal Mursyid Djawas Murti, Sri Djangkung Sumbogo Nabila, Annisa Candra Nadia Rahmasari Nani Lestari Natasya, Dian Nelson Nelson Nelson Nelson Nelson Nelson Nelson Nelson Nelson Nelson Nelson Nelson Ngatijo Ngibad, Khoirul Niken Rarasati Nindita Clourisa Amaris Susanto Nindita Clourisa Amaris Susanto Novintan Elystia Nugraha, A. Prima Nugroho, Romy Triadi Nuralang Nuralang Nuralang Nuralang nuralang, nuralang Nurjannah, Sofia Nursofia, Yesi Nurul Huda Oktavioni, Marsella Patricia, Anggun Dwi Prabawa, Satria Prahandika, Graha Pratiwi, Putri Indah Puspitasari, Ratih Dyah Puspitasari, Ratih Dyah Puspitasari Putra, Adrisma Juanda Putra, Surya Andika Putri Maya Sari Putri Maya Sari Putri Maya Sari Putri Pebridayanti Putri, Alya Adiningtyas Putri, Silvi Ayudiah Putri, Yunika Eka qurrota aini Rahayu, Martina Asti Rahmadani Ramadhan, Iham Ifandi Ramadhan, Ilham Ifandi Ramlan Silaban Ratih Dyah Puspitasari Razai, Tengku Said Reski, Alpi Meitia Restina Bemis, Restina Ricardo Lumbantoruan Rizki Ananda Rizqi Asri Fauzi Nugraha Rosi Rahayu Safitri, Ayu Nadila Saharin, Siti Munirah Saputra, Ardiyanto Saputra, Gilang Saputra, Muhammad Furqon Novryan Sarah Aknesia Marbun Sari, Tika Seow, Eng Keng Septina Is Heriyanti Shadrina, Putri Nur Sihotang, Andreas Silaban, Desy Vania Silvi Leila Rahmi Sinurat, Julius Alvin Novensius Sitanggang, Boy Candra Siti Aisyah Sofia Nurjannah Sonia Sonia, Sonia Sowe, Momodou Salieu Sri Djangkung Sumbogo Murti Suci Lestari Suci, Rasyifa Sulistiara, Ely Susanto, Nindita Clourisa Amaris Susanto, Nindita Clourisa Clourisa Amaris Sutrisno Sutrisno, Sustrisno Sutrisno, Sutrisno Syahrum, Silvina Faradilla Taufik Salis Syaifudin Tiara Aninditha Tilarso, Dara Pranidya Tri Kurnia Astutik Tri Siswanto Tusa’diah, Halimah Uce Lestari Uce Lestari Vitadella, Veronika Wahyuni, Linda Warni, Tri Wasistha, Excel Emerlan Samiar Wazzan, Huda Widy Prasetyo Wiwik Handayani Yana Cahyana Yoga Andika Yuliantari, Sonia Devi Yulianti, Nike Desvi Yusnaidar Yusnaidar Yusnaidar, Yusnaidar Yusof, Yus Aniza Zaki, Nesfitra Dzakwan Zulnita, Anis