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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Veteriner Jurnal Ilmu Ternak Zuriat Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Science and Technology Indonesia Animal Production : Indonesian Journal of Animal Production Jurnal Ilmu Nutrisi dan Teknologi Pakan ANNALES BOGORIENSES Jurnal Aplikasi Teknologi Pangan Jurnal Ilmiah Fillia Cendekia Building of Informatics, Technology and Science ZIRAA'AH MAJALAH ILMIAH PERTANIAN Indonesian Journal of Electrical Engineering and Computer Science Jurnal Ilmiah Sains Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Agripet Animal Production Annales Bogorienses
Cece Sumantri
Department Of Animal Production And Technology, Faculty Of Animal Science, IPB University, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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SNP Discovery of Chicken Liver with Divergent Unsaturated Fatty Acid using Next Generation RNA Sequencing Asep Gunawan; Mutasem Ali M. Abuzahra; Kasita Listyarini; Jakaria Jakaria; Cece Sumantri
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.625 KB) | DOI: 10.33772/jitro.v6i1.5807

Abstract

ABSTRAKRNA sequencing memberikan peluang baru untuk mendeteksi variasi SNP (Single Nucleotide Polymorphism) pada perbedaan jaringan dengan perbedaan fenotipe. Tujuan dari penelitian ini adalah untuk mengkarakterisisasi penemuan SNP terbaru terkait perbedaan asam lemak tak jenuh pada ayam dengan menggunakan RNA sequencing. Sebanyak 6 sampel dipilih dari 62 sampel masing-masing 3 sampel tinggi dan 3 sampel rendah  yang merepresentasikan perbedaan fenotip yang kontras terkait asam lemak tak jenuh dianalisis dengan menggunakan RNA Sequensing. Hasil identifikasi SNP memperlihatkan  sebanyak 1208 SNP pada sampel tinggi dan rendah setelah disejajarkan dengan genom ayam Gallus gallus (GGA) v4.0. Sekitar 91% dari total SNP yang ditemukan memiliki tingkat polimorfisme yang tinggi pada 5 gen yang ditemukan terkait asam lemak yaitu gen SCD, COL6A2, CYP2J2L4, HSD17B4, dan SLC23A3. Gen SCD, HSD17B4, dan SLC23A3 memiliki jumlah titik mutasi dengan jumlah yang paling tinggi masing-masing berturut-turut  18, 13, dan 12 SNP. Tingkat level signifikan yang tinggi dan peranan dari ketiga gen tersebut yang sangat penting terkait komposisi asam lemak mengindikasikan bahwa gen SCD, HSD17B4, dan SLC23A3 merupakan tiga gen baru dan potensial untuk digunakan sebagai penanda seleksi kandungan asam lemak tak jenuh tinggi. Namun, hasil penelitian ini perlu divalidasi dan dikonfirmasi sebagai potensial kandidat gen dalam jumlah ayam yang lebih besar dan breed yang berbeda.Kata kunci: asam lemak, ayam, RNA-Seq, variasi transkriptomikABSTRACTRNA sequencing (RNA-Seq) reveals new opportunity for identification  SNP discovery in different tissues with divergent phenotype. The objective of this study was to characterize SNP profile from divergent unsaturated fatty acids using RNA-Seq. Six liver samples were selected from 62 chicken which classified 3 high and 3 low unsaturated fatty acids were analyzed using RNA-Seq. The SNP identification showed 1208 SNPs in chicken samples and a large number of those corresponded to differences between high and low chicken genome assembly Gallus gallus (GGA) v4.0. Among them, about 91% of genes had multiple polymorphisms within 5 genes (SCD, COL6A2, CYP2J2L4, HSD17B4, and SLC23A3). The SCD, HSD17B4, and SLC23A3 contained the largest number of mutations with 18, 13, and 12 SNPs respectively. Combining the significant level of SNPs and gene function related with fatty acid composition  allow us to suggest SCD, SLC23A3, HSD17B4 as the three novel and promising candidate genes for selecting unsaturated fatty acids. However, further validation is required to confirm the effect of these candidate genes in larger chicken populations.Keywords: chicken, fatty acids, RNA-Seq, transcriptome variants
Pengaruh Konsentrasi IgY Induk yang Berbeda Terhadap Gambaran Darah Anak Ayam Sentul Melly Pratiwi Setyawati; Niken Ulupi; Sri Murtini; Cece Sumantri
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 2 (2019): JITRO, Mei
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.147 KB) | DOI: 10.33772/jitro.v6i2.7609

Abstract

ABSTRAKPenelitian ini bertujuan untuk menganalisis gambaran sel darah putih dan sel darah merah sebagai indikator ketahanan tubuh pada anak ayam sentul. Penelitian ini menggunakan 20 ekor ayam betina dan 20 ekor ayam jantan yang dikawinkan, masing masing terdiri dari 10 ekor ayam dengan konsentrasi IgY tinggi dan 10 ekor ayam dengan konsentrasi IgY rendah. Berdasarkan hasil perkawinan tersebut diperolah 34 ekor anak ayam yang terdiri dari 17 ekor anak ayam yang berasaldari induk dengan konsentrasi IgY rendah. Parameter yang diamati meliputi gambaran sel darah merah (eritrosit, hematocrit dan indeks eritrosit) dan gambaran sel darah putih (leukosit dan diferensiasinya). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 2 perlakuan, semua perlakuan diulang 10 kali. Data yang diperoleh dianalisis menggunakan t-test program SAS.  Hasil penelitian ini menunjukan bahwa perbedaan konsentrasi IgY pada induk tidak mempengaruhi sel darah merah dan sel darah putih. Namun terdapat kecendrungan nilai limfosit yang rendah dan H/L yang tinggi pada anak ayam yang berasal dari induk dengan konsentrasi IgY rendah. Hasil ini mengindikasi bahwa anak ayam yang berasal dari induk dengan konsentrasi IgY rendah lebih rentan terhadap cekaman panas. Kata Kunci: ayam sentul, IgY,  gambaran darah, ketahanan tubuhABSTRACTThis study was to aim the description of white blood cells and red blood cells as indicators of immunity in Sentul chicks. This study used 20 hen chickens and 20 cock-chicken mated, consisting of 10 chickens with high IgY concentration and 10 chickens with low IgY concentrations. Based on the results of mating, 34 chicks were obtained consisting of 17 chicks that were from the hen with low IgY concentration. The parameters observed included the description of red blood cells (erythrocytes, hematocrit and erythrocyte index) and the description of white blood cells (leukocytes and their differentiation). The experiment was conducted as a completely randomized design with 2 treatments, all treatments were repeated 10 times. The data obtained were analyzed using the SAS program t-test. The results of this study indicate that the difference in IgY concentration in the hen did not effect on red blood cells and white blood cells. However, there is a tendency for low lymphocyte values and high H/ L in chicks originating from hens with low IgY concentrations. These results indicate that chicks from hens with low IgY concentrations are more susceptible to heat stress.Keyword: blood picture, IgY, immunity, sentul chicken
Mikroklimat, Termoregulasi dan Produktivitas Sapi Perah Friesians Holstein pada Ketinggian Tempat Berbeda Elmy Mariana; Cece Sumantri; Dewi Apri Astuti; Anneke Anggraeni; Asep Gunawan
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.944 KB) | DOI: 10.33772/jitro.v6i1.5617

Abstract

ABSTRAKTujuan penelitian ini adalah untuk mengetahui pengaruh ketinggian tempat terhadap kondisi mikroklimat,termoregulasi dan produktivitas sapi Friesian-Holstein (FH). Penelitian dilakukan pada tiga lokasi dengan ketinggian berbeda, yaituPodok Ranggon (97mdpl), Ciawi (576mdpl), dan Lembang (1241mdpl). Sebanyak 63 sapi FH dalam kondisi laktasi normal digunakan dalam penelitian ini.  Aspek lingkungan meliputi suhu udara, kelembaban relatif dan Temperature-Humidity Index di dicatat setiap 2 jam dari pukul 08.00 sampai 16.00 WIB. Parameter termoregulasi yang diamati terdiri atas suhu kulit (Ts),suhu rektal (Tr), suhu tubuh (Tb), denyut jantung (Hr),laju pernapasan (Rr), dan Heat Tolerance Coeficient (HTC).Berdasarkan nilai Ta, Rr dan THI, dataran rendah memberikan dampak cekaman panas sedang, dataran sedangdan tinggi memberikan dampak cekaman panas ringan pada sapi perah. Hasil menunjukkan bahwa sapi dataran rendah memiliki Nilai HTC, Tr, Ts dan Tb tertinggi (P<0,05) dan Hr yang terendah (P<0,05). Produksi susu di ketiga lokasi penelitian berbeda nyata (P<0,05), dengan produksi susu tertinggi di dataran tinggi (13,1±3,52 kg),dataran sedang (11,3±4,73 kg) dan dataran rendah (7,0±3,36 kg). Secara umum sapi FH di dataran rendah, sedang dan tinggi tercekam panas akibat kondisi lingkungan yang berada pada kondisi di luar zona nyamanselama musim kemarau, akan tetapi mampu beradaptasi terhadap kondisi tersebut. Kata kunci: ketinggian tempat, produktivitas, sapi perah, cekaman panas, termoregulasiABSTRACTThe purpose of this research was to determine altitude effect on microclimate, thermoregulation and productivity of Friesian Holstein. Research was conducted in different altitudes, i.e.: Pondok Ranggon (97m asl), Ciawi (576m asl), and Lembang (1241 m asl). A total of 63 FH cows in normal lactation were used in this study. Microclimate aspects observation includes environmental temperature (Ta), relative humidity (RH) and Temperature-Humidity Index (THI) were recorded every 2 hours from 08.00 to 16.00. The physiological responses measurements consisted of skin temperature (Ts), rectal temperature (Tr), body temperature (Tb), heart rate (Hr), respiratory rate (Rr) and Heat Tolerance Coeficient (HTC). Lowland environmental provide moderate heat stress, while the medium and highland impacts with mild stress. The results showed that lowland cows have highest HTC, Tr, Ts and Tb (P<0.05) and lowest Hr (P<0.05). Milk production in the different altitude was significantly different (P<0.05), with the highest milk yields in the highlands (13.1±3.52 kg), medium (11.3±4.73 kg) and lowland (7.0±3.36 kg). In general, HF dairy cows in low-, medium- and highland are exposed to climatic stress during dry season conditions, although they have the ability to adapt physiologically and cope with environmental stress.  Keyworlds: altitude, dairy cattle, heat stress, productivity, physiological responses
Identifikasi Keragaman Gen FTO Pada Bangsa Sapi Potong Indonesia Sutikno Sutikno; Rudy Priyanto; Cece Sumantri; Jakaria Jakaria
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 2 (2019): JITRO, Mei
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.158 KB) | DOI: 10.33772/jitro.v6i2.5655

Abstract

ABSTRAK Gen FTO berfungsi sebagai regulasi homeostasis, deposisi lemak dan pengaturan obesitas. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme SNP g.125550A>T di ekson 3 gen FTO pada bangsa sapi potong Indonesia. Sampel darah diperoleh dari 209 ekor sapi, terdiri atas sapi bali (44), madura (20), Pesisir (20), katingan (20), Peranakan ongole (PO) (22), Pasundan (20), Sumba Ongole (SO) (11), brahman (20), simental (15), dan limousin (18). Polimorfisme gen FTO dianalisis menggunakan metode PCR-RFLP (HpyCH4III) dan direct sequencing. Hasil genotiping SNP g.125550A>T adalah polimorfik (genotipe AA, AT, dan TT) pada sapi madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, dan limousin. Frekuensi alel A dan T masing-masing adalah 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 dan 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31. Nilai Ho dan He masing-masing adalah 0,60-0,14 dan 0,44-0,18 serta dalam keseimbangan Hardy-Weinberg (P>0.05). Sementara pada sapi bali bersifat monomorfik hanya bergenotipe AA. Hasil sekuensing SNP g.125550A>T ditemukan mutasi tranvesi A menjadi T pada posisi nukleotida  g.125550. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa SNP 125550A>T gen FTO beragam dan berpotensi dijadikan marka genetik untuk kualitas daging pada bangsa sapi potong Indonesia.Kata Kunci: gen FTO, PCR-RFLP, Sapi, SNP g.125550A>TABSTRACTThe FTO gene functions as regulation of homeostasis, fat deposition and regulation of obesity. This study aimed to identify the polymorphism of SNP g.125550A>T in exon 3 of FTO gene in Indonesian beef cattle. Blood samples were collected from 209 cattle, including bali (44), madura (20), pesisir (20), katingan (20), PO (22), pasundan (20), SO (11), brahman (20), simental (15), and limousin (18). Polymorphism of the FTO gene was analyzed using PCR-RFLP (HpyCH4III) and direct sequencing methods. The results of genotyping SNP g.125550A>T was polymorphic (AA, AT and TT genotypes) in madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, and limousin cattle. The frequency of A and T alleles were 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 and 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31 respectively. The values of Ho and He were 0,60-0,14 and 0,44-0,18 respectively and in Hardy-Weinberg equilibrium (P>0,05). While in Bali cattle was monomorphic (AA genotype). Results of sequencing SNP g.125550A>T of the FTO gene found a transverse mutation A to T at the nucleotide position g.125550. As a result of this study, it can be concluded that SNP 125550A>T of the FTO gene was diverse and potentially used as genetic markers for meat quality in Indonesian beef cattle.Keywords: cattle, FTO gene, PCR-RFLP, SNP g.125550A>T.
Production Performance, Reproduction and Immunity of Sentul Hens at Different IgY Concentrations Melly Pratiwi Setyawati; Niken Ulupi; Sri Murtini; Cece Sumantri
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.35180

Abstract

The aim of this study was to evaluate the effect of different IgY concentrations on the production, reproduction and immunity of Sentul chicken. This research used 20 month-old Sentul chickens which consisting of 10 chickens with high IgY concentration and 10 chickens with low IgY concentration Parameters observed included prelayer performance (feed consumption, weight gain, feed conversion, morbidity and mortality), layer performance (feed consumption, egg production, egg weight, feed conversion, hatchability, morbidity and mortality), and immunity at chicks (IgY concentration of eggs and chicks serum). The experiment was conducted as a completely randomized design. The treatments were high and low IgY concentration. All treatments were repeated 10 times. The data obtained were analyzed using the SAS program t-test. Morbidity and mortality were analyzed descriptively. The results of this research indicated that the prelayer period, chickens with high IgY concentration had a higher performance than chicken with low IgY concentration. This is caused by about 30% chickens with low IgY were in sick condition for approximately seven days. Further, the layer period showed that the chickens with high IgY concentration had lower production performance than the chicken with low IgY concentration. The study concluded that chickens at low IgY concentrations are susceptible to exposure to antigens that lead to decreased productivity. In normal conditions hens, Sentul with high IgY concentration resulted in low production. The observation of chicken immunity showed that concentration of IgY from chicks and eggs did not differ between hens with high IgY concentrations and hens with low IgY concentrations.
Thermoregulation, Haematological Profile and Productivity of Holstein Friesian Under Heat Stress at Different Land Elevations Elmy mariana; Cece Sumantri; Dewi Apri Astuti; Anneke Anggraeni; Asep Gunawan
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.37648

Abstract

The purpose of this research was to determine the effect of heat stress on thermoregulation, haematology, and productivity of Holstein Friesian (HF) dairy cows raised in different elevations. A total of 63 HF in a normal lactation period were used in this study. The research was conducted for 3 months during dry season in three different areas, which were at Pondok Ranggon (97 m.a.s.l) which categorized as a lowland, Ciawi (576 m.a.s.l) which categorized as a lower-upland, and Lembang (1241 m.a.s.l) which categorized as an upland. Observation on microclimate aspects which includes environmental temperature (Ta), relative humidity (RH) and Temperature-Humidity Index (THI) was done by recording each variable for every 2 hours starting from 08.00 to 16.00 WIB. The thermoregulation analysis was done based on the given physiological responses which consisted of the skin temperature (Ts), rectal temperature (Tr), body temperature (Tb), heart rate (Hr), respiratory rate (Rr), Heat Tolerance Coefficient (HTC), plasma cortisol level and haematological profile observation. The Ta, Rr, and THI measurements showed that in the lowland and lower-upland, the HF experienced moderate heat stress, while the HF raised in the upland area experienced less heat stress. The results showed that the dairy cows which raised in lowland had the highest HTC, Tr, Ts and Tb (P<0.05) and lowest Hr (P<0.05). All of the physiological and haematological parameters in the three study area showed a normal value.Furthermore, the erythrocyte, Hb and PVC concentration in a lowland raised HF were higher (P<0.05), while the plasma cortisol levels were not significantly different. The milk production of the observed dairy cows in different elevations was significantly different (P<0.05), with the highest milk yields, were found in the upland raised HF (13.1±3.52 kg), followed by the lower-upland (11.3±4.73 kg) and lowland (7.0±3.36 kg). In general, all of the HF raised in different land elevations was exposed to heat stress during dry seasons, even though the cows showed the ability to physiologically adapt and cope with the conditions. 
Physicochemical Characteristics and Microbiological Quality of the Topside and Longissimus Dorsi of Indonesian Local Buffalo Meat Noraimah Binti Sulaiman; Cece Sumantri; Irma Isnafia Arief; Cahyo Budiman
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.45544

Abstract

The physicochemical characteristics and microbiological quality of buffalo meat are influenced by differences in muscle type. This study aimed to evaluate the physiochemical characteristic and microbiological quality of the topside (active muscle) and longissimus dorsi (passive muscle) of Indonesian local buffalo meat. Samples used in this study were buffalo meat from local swamp buffalo, aged more than four years old on the topside and longissimus dorsi. This study used a completely randomized design, with three repetitions in each treatment. All data were analyzed using analysis of variance (ANOVA). The result of the study on the topside and longissimus dorsi area showed a significant difference in the pH and cholesterol levels of the buffalo meat. The longissimus dorsi area had a lower level of pH and cholesterol compared to the topside area. Furthermore, this longissimus dorsi meat has a higher color, protein, ash, fat, essential amino acid, and lactic acid bacterial (BAL) content than the topside meat. However, the topside meat had higher carbohydrate, essential fatty acid, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) content compared to the longissimus dorsi meat. Longissimus dorsi meat had better physicochemical characteristics and microbiological quality than the topside meat
Association of Cytochrome P450 2A6 (CYP2A6) Gene Polymorphisms with Fatty Acid Traits in Indonesian Native Sheep Gilang Kurnia Abdillah; Kasita Listyarini; Ratna Sholatia Harahap; Asep Gunawan; Cece Sumantri
Buletin Peternakan Vol 45, No 3 (2021): BULETIN PETERNAKAN VOL. 45 (3) AUGUST 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i3.65604

Abstract

A member of the cytochrome P450 superfamily enzyme that play a role in microsomal fatty acids oxidation process encoded by cytochrome P450 2A6 (CYP2A6). This study aimed to examined the CYP2A6 gene polymorphisms and their association on fatty acid traits in Indonesian native sheep. A total of 299 rams aged 10-12 months with body weights between 20-30 kg used to identify the CYP2A6 gene polymorphism are consisted of 36 samples of barbados cross sheep (BCS), 35 samples of compass agrinak sheep (CAS), 20 samples of javanese fat tailed sheep (JFT), 36 javanese thin tailed sheep (JTT), 20 samples of garut sheep (GS), 45 samples of garut composite sheep (GCS), meanwhile for the fatty acid analysis was carried out by using 107 of loin samples from the total sheep. Identification of the CYP2A6 gene polymorphisms were performed using PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) with the BSmAI restriction enzymes. The amplification product was 286 bp. Polymorphism were found in JFT, JTT, GCS and JS with GG and GT genotypes, while BCS, CAS, and GS were monomorphic with TT genotype. The CYP2A6 BSmAI polymorphism was in Hardy-Weinberg Equilibrium for JFT, JTT and JS, while BCS, CAS, GS, GCS, and combined was deviated based on chi square. A SNP g.49170107 G>T of the CYP2A6 gene polymorphism was significantly associated (P <0.05) with only erucic acid (C22:1n9). The GT genotypes had a higher value than GG genotypes The CYP2A6 gene could be used as a selection marker to improve fatty acid traits in Indonesian native sheep.
Determination of Morphological Characteristics in Kuantan Cattle using Multivariate Analysis Restu Misrianti; Jessy Mainidar; Hasrul Bani Asharudin; Yureni Sahril Dedi; Arsyadi Ali; Sony Hartono Wijaya; Cece Sumantri; Jakaria Jakaria
Buletin Peternakan Vol 45, No 3 (2021): BULETIN PETERNAKAN VOL. 45 (3) AUGUST 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i3.66868

Abstract

The objective of this research was to characterized morphology and estimated genetic distance between intra population of Kuantan cattle. A Total of 213 cattle (44 male and 169 female with age ranging from 2-3 years) were used in this study and collected from extensive ranging systems in Three sub-population (Cerenti, Inuman, and Kuantan Hilir regions) Kuantan Singingi Regency, Riau Province. Five variables were measured that is Body Length (BL)(cm), Wither Height (WH)(cm), Hip Height (HH)(cm), Chest Girth (CG)(cm), and Chest Depth (CD)(cm). Data obtained were descriptive analysis, Principal Components Analysis (PCA) and Hierarchichal Clustering Analysis (HCA)  using XLSTAT program. All variables of body measurement in the Kuantan Hilir region were higher than Cerenti dan Inuman, Kuantan Singingi Regency. The first factor in PCA described body measurement contributed 32.77%, and the second factor described body shape contribute 25.83% of total variability. The dendrogram showed there is Three clusters of Kuantan Cattle based on this research.
Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle Winni Liani Daulay; Putri Indah Ningtias; Cece Sumantri; Jakaria Jakaria
Buletin Peternakan Vol 46, No 1 (2022): BULETIN PETERNAKAN VOL. 46 (1) FEBRUARY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i1.69784

Abstract

Investigating Myostatin (MSTN) as a potent inhibitor of skeletal muscle growth and development to produce excessive muscles is extremely essential for livestock breeding. This study aimed to analyze the expression of the MSTN gene and its relationships with genotype and phenotype (normal-muscled vs double-muscled) of Belgian Blue (BB) x Ongole Grade (PO) crossbred cattle. For that purpose, 12 animals from BB, PO, BB x PO F1, and BB x PO F2 cattle (3 animals each) raised at Balai Embrio Ternak (BET) Cipelang Bogor, West Java were used for blood sample collection. Genotyping analysis was performed using the PCR-RFLP method withprimer F: 5’-CTC TTC TTT CCT TTC CAT ACA GAC-3’ and R: 5’-AGG GGA AGA CCT TCC ATG TT-3’, while the MSTN gene expression was analyzed using the qPCR technique. As results, three genotypes: del.11/del.11, +/del.11, and +/+ were detected. The del.11/del.11 genotype, which showed a double-muscled phenotype was found in BB cattle and BB x PO F2 cattle. The +/del.11 genotype was found in BB x PO F1 cattle and BB x PO F2 cattle. The +/+ genotype, which showed a normal phenotype was only detected in PO cattle. There was a significant difference of the MSTN gene expression in the sampled animals among genotypes and between phenotypes (normal-muscled vs double muscled). The MSTN expression in animals with del.11/del.11 genotype was higher than that in animals with +/del11 and +/+ genotypes (P<0.05). Animals with +/+ genotype showed the lowest MSTN expression. It was concluded that double-muscled animals showed higher MSTN expression than normal-muscled animals.
Co-Authors . Hidayati . Subandriyo A Anggraeni A Anggraeni A B Dharmayanthi A Gunawan A M Fuah A.B.L. Ishak A.B.L. Ishak Abdul Samad ACHMAD FARAJALLAH Achmad Gitaraka Archimilar Adi Teruna Effendi Agus Buono Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Romadhoni Surya Putra ahmad yani Akbanugra Yudhananda Al Mukhlas Fikri Aldri Frinaldi Amalia Rahma Aminuyati Andi Baso Lompengeng Ishak Anggraeni Anggraeni Anggraeni, A. Anggraeni Ani Rosmayanti Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Any Aryani Arief Boediono Aron Batubara Asep Anang Asep Gunawan ASEP SAEFUDDIN Asmarasari, Santiananda Arta Astira, Adinda Wanda B Purwantara Bambang Purwantara Ben J. Takaendengan Ben J. Takaendengan Bramada Winiar Putra Budiariati, Vista Cahyo Budiman Cesilia M Dwiriani Chalid Talib Chalid Talib Clara Meliyanti Kusharto D D Solihin D, Nurhayati Dadan Rohdiana Dairoh Dairoh Dairoh Dairoh, Dairoh Darlian Fachruddin Dedi Duryadi Solihin Dedi Rahmat Depison Depison Depison Depison DEWI APRI ASTUTI Diah Nugrahani Pristihadi Dudi Syadili Dwi Budiono Dwi Margi Suci E Andreas E Taufik E. Andreas E. Andreas E. Damayanthi E. Damayanthi E. I. Rianti E. I. Rianti, E. I. Eko Handiwirawan Eko Handiwirawan Elmy mariana Elmy Mariana Epi Taufik Eryk Andreas Evy Damayanthi Fadhilah Dhani Santika Falah Farajallah, Ahmad Ferdian Achmad Fisma Amri Gilang Kurnia Abdillah Gina, Safira Güler, Serdar Gushairiyanto Gushairiyanto Hadi Riyadi Harahap, Ahmad Saleh HARIMURTI MARTOJO Harini Nurcahya Mariandayani Hasrul Bani Asharudin Heni Rizqiati Heni Rizqiati Henny Nuraini herdis herdis Herpina Herpina Hidayat, Numan I Khaerunnisa I Wayan Mathius I wayan Teguh Wibawan Iman Supriatna Imas Batubara Irma . Irma . Irma Herawati Suparto Irma Irma Irma Isnafia Arief Irmanida Batubara Ishak, Andi Baso Lompengeng Ismeth Inounu Ismeth Inounu Isyana Khaerunnisa Isyana Khaerunnisa Iwan Prihantoro Jakaria Jakaria Jakaria Jakaria Jarmuji Jarmuji Jessy Mainidar Johar Arifin Joni Setiawan Junaedi Junaedi Junaedi Junaedi K Kinoshita Katrin Roosita Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komang Alit Paramitasari Komariah . Kuswandi, Wawan Laelatul Choiriyah Lailatul Rohmah Lia Budimulyati Salman Lilis Nuraida Listyarini, Kasita Luci Cyrilla M Imron M S Engel M. Restu Mala Nurilmala Maskur Maskur Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Mochammad Sriduresta Soenarno Mohamad Hasil Tamzil Mohamad Yamin Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muh. Rusdin MUHAMMAD AGIL Muhammad Alwi Muhammad Fikri Al Habib Muhammad Suhendra, Muhammad Muhsinin, Muhammad Mutasem Ali M. Abuzahra N Hamashima N Hamashima Nena Hilmia Nensy Tri Putri Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Niswati, Za'imatun Noer Muhammad Dliyaul Haq Noraimah Binti Sulaiman Novita Nur Anggraeni Noviyan Darmawan Nurhayati D Nurhayati, Nurhayati D. Nu’man Hidayat Okti Nadia Poetri Olfa Mega Oloan Parlindungan Pardede, Berlin Pandapotan Pramujo, Muhammad Pratama, Bondan Purwantiningsih Sugita Puspita, Poppy Satya Putri Indah Ningtias R Diyono R Priyanto R. Iis Arifiantini Rahmagiarti, Cintera Rarah Ratih Adjie Maheswari Ratna Sholatia Harahap Ratnawati, Diana Raudah Putri Ekawati Refdanita, Refdanita Restu Misrianti Retno Setyaningsih Rina Martini Rini Herlina Mulyono Rita Mutia Romantis, Sara Rona Saumy Safitry Ronny R. Noor Ronny R. Noor Ronny Rachman Noor Ronny Rahman Noor RR Noor Rudi Afnan Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih S Darwati S Kahono S Sopiyana Saiful Anwar Salatnaya, H. Salsabila Ma’shum Imawan Santi Ananda Asmarasari Santiananda Arta Asmarasari Santoso Santoso Sarah Tazkya SATRIYAS ILYAS Septaria Jodiansyah Shidiq, Fajrin Sigid Prabowo Sikin Sikin Simon Elieser Siti Darodjah Siti Zubaidah Slamet Mulyanto Sony Hartono Wijaya Sri Adiani Sri Adiani Sri Darwati Sri Darwati Sri Mulatsih sri murtini . Sri Nurdiati SRI RAHAYU Sri Sulandari Sri Wahyuni Siswanti Sri Wahyuni Siswanti Subandriyo . . Sugyono . Sumiati Sumiati Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syah, Setiawan Putra Syamsul Arifin T Akiyama T Morzumi T. Suryati TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tatan Kostaman TIKE SARTIKA Tike Sartika Triana Susanti Triana Susanti Tuti Suryati Tuti Suryati Tuty Laswardi Yusuf Ummah, Isna Mustafiatul Vianisa, Putri Wasmen Manalu Weni Kurniati Widiatmaka Widya Pintaka Bayu Putra Wike andre Septian Winni Liani Daulay Yong Soo Kim Yulianto Yulianto Yun Alwi Yureni Sahril Dedi Yusman Setiawan Yusuf, Salman Zakiah Wulandari Zuraida Hanum Zuraida Hanum Zuraida Hanum