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All Journal International Journal of Electrical and Computer Engineering Torani Journal of Fisheries and Marine Science Lintas Ruang: Jurnal Pengetahuan dan Perancangan Desain Interior Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Jurnal Ilmiah Mahasiswa FEB Journal of Biomedicine and Translational Research Jurnal Ilmu Kelautan Spermonde JURNAL IPTEKS PEMANFAATAN SUMBERDAYA PERIKANAN Biomedical Journal of Indonesia Sinkron : Jurnal dan Penelitian Teknik Informatika Medical Laboratory Technology Journal Pendas mahakam : jurnal pendidikan dan pembelajaran Sekolah Dasar JURNAL MUQODDIMAH : Jurnal Ilmu Sosial, Politik dan Hummaniora Jurnal Ilmiah Mahasiswa Kedokteran Medisia Jurnal Pendidikan Matematika (JUDIKA EDUCATION) Fair Value: Jurnal Ilmiah Akuntansi dan Keuangan Jurnal Teknologi Sistem Informasi dan Aplikasi JURNAL PENDIDIKAN TAMBUSAI SRIWIJAYA JOURNAL OF MEDICINE ACTIVE: Journal of Physical Education, Sport, Health and Recreation Jurnal Teknologi Terpadu Jurnal Logistik Indonesia Bioscientia Medicina : Journal of Biomedicine and Translational Research Jurnal Menssana Jurnal Mantik Riyadhoh : Jurnal Pendidikan Olahraga Imajeri: Jurnal Pendidikan Bahasa dan Sastra Indonesia Scientia Psychiatrica Journal of SPORT (Sport, Physical Education, Organization, Recreation, and Training) REMIK : Riset dan E-Jurnal Manajemen Informatika Komputer Jurnal PORKES RESLAJ: RELIGION EDUCATION SOCIAL LAA ROIBA JOURNAL Sriwijaya Journal of Surgery Open Access Indonesia Journal of Social Sciences Sriwijaya Journal of Ophthalmology JURNAL PENDIDIKAN SAINS SOSIAL DAN AGAMA Eureka Herba Indonesia Jurnal Kesehatan Tambusai Jurnal Pengabdian Masyarakat: Humanity and Medicine PRAJA observer: Jurnal Penelitian Administrasi Publik Natural Sciences Engineering and Technology Journal Oikonomia: Jurnal Manajemen Open Access Indonesian Journal of Medical Reviews Indonesian Community Empowerment Journal Jurnal Abdimas Bina Bangsa Archives of The Medicine and Case Reports Bioscientia Medicina : Journal of Biomedicine and Translational Research Open Access Indonesia Journal of Social Sciences Arkus Abditeknika - Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Kepada Masyarakat Patikala International Journal of Research and Applied Technology (INJURATECH) Muhasabatuna: Jurnal Akuntansi Syariah Journal of Informatics and Communication Technology (JICT) Journal of Anesthesiology and Clinical Research Journal Of Human And Education (JAHE) Scriptura : Jurnal Ilmiah Sustainability Science and Resources Journal International of Lingua and Technology Proceeding ISETH (International Summit on Science, Technology, and Humanity) Indonesian Journal Computer Science (ijcs) Journal Health Information Management Indonesian Indonesian Research Journal on Education Dikdimas : Jurnal Pengabdian Kepada Masyarakat Jurnal Muara Olahraga Jurnal Multidisiplin Sahombu Jurnal Pengabdian West Science Sinergi International Journal of Communication Sciences Jurnal Sosial, Sains, Terapan dan Riset (Sosateris) Technology and Society Perspectives (TACIT) Scientific Journal of Pediatrics Jurnal Spektrum Komunikasi Sriwijaya Journal of Radiology and Imaging Research Journal of Social Science Utilizing Technology Sriwijaya Journal of Internal Medicine Sriwijaya Journal of Obstetrics and Gynecology Sriwijaya Journal of Otorhinolaryngology Jurnal Pengabdian Sosial Informatics and Software Engineering (ISE) Jurnal Perikanan Terpadu Journal of Informatics and Communication Technology (JICT) Journal of Social Science Utilizing Technology Journal of Industrial Engineering Innovation Acta Psychologia ACTIVE: Journal of Physical Education, Sport, Health and Recreation Communica : Journal of Communication Bambu Laut: Jurnal Pengabdian Masyarakat Journal of Legal Contemplation Lentera: Journal of Islamic Studies
RACHMAT HIDAYAT
Department Of Medical Biology, Faculty Of Medicine, Universitas Sriwijaya, Palembang, Indonesia
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Other

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Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 4 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v5i4.229

Abstract

Western blotting is an important technique used in cell and molecular biology. Using the western blot, researchers can identify specific proteins from the complex mixture of proteins extracted from cells. This technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target proteins using appropriate primary and secondary antibodies to visualize. This paper will attempt to explain the techniques and theory behind western blot, and offer several ways to solve the problem
Preclinical Test Methods with Animal Mouse and Rats Biomedical Research: Technical Guideline Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 2 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i2.232

Abstract

A B S T R A C TTrials in pre-clinical trials are very complex multidisciplinary studies. Extrapolatingdata from animals to humans requires information from many fields of science. Frompre-clinical trials, more detailed information and data on efficacy and safety wereobtained, especially at doses equivalent to doses in humans, and the presence orabsence of cumulative effects and whether these effects could return to normal(reversible) after the administration of the test material was stopped. This test canbe used to predict the negative impact on humans if he is exposed to the materialfor a long time.
Euthanasia Procedure of Animal Model in Biomedical Research Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 6 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i6.310

Abstract

Regardless of the method, it is essential to induce unconsciousness as rapidly as possible if euthanasia is to be aesthetically and scientifically successful. Criteria that have been considered in recommending the methods of euthanasia include: time required to produce unconsciousness, time required to produce death, purposes, research results and compliance with the AVMA Guidelines on Euthanasia. There are special considerations for euthanizing rodent embryos, fetuses and neonates.
Protocol for Anesthesia Animal Model in Biomedical Study Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 7 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i7.311

Abstract

Recognition of pain depends upon intact pathways from pain receptors to the thalamus and cerebral cortex, as well as functional cerebral cortex and subcortical structures. Thus any means that renders the cerebral cortex nonfunctional, such as hypoxia or drug depression, prevents pain. When this happens, stimuli that evoke motor nerve reflexes that may be painful to the conscious animal are not painful in the unconscious animal. Equally painful stimuli administered to animals chemically paralyzed by curare or succinylcholine will not evoke a motor reflex simply because of paralysis, but will cause pain because of the conscious state. Hence, it is possible that unconscious animals may feel no pain but respond to certain stimuli, and paralyzed animals may feel pain but cannot respond.
Fluid and Drug Administration Procedure Animal Model in Biomedical Research Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 8 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i8.312

Abstract

Drugs, vaccines, injectable anesthetics or other agents are to be administered, one or more of several different routes may be selected. The routes selected are governed by the nature of the agent being administered, the animal, the purpose of the administration and other factors.
Handling of Common Laboratory Animals in Biomedical Study Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 9 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i9.313

Abstract

Mice are usually caught and lifted by the tail. The tail should be grasped abouttwo-thirds of the way down. With this simple method of holding, they may betransferred to another cage or a balance, identified or sexed; but such restraint isnot sufficient for treatment and close examination. For more effective control, themouse may be held by the tail and placed on a table or other surface, preferablyone that the mouse can grasp, and the loose skin over the neck and shouldersgrasped with thumb and fingers.
Cancer Treatment in Islamic Traditional Medicine Rachmat Hidayat
Arkus Vol. 7 No. 2 (2021): ARKUS
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/arkus.v7i2.93

Abstract

Although significant progress has been made in cancer treatment in recent decades, the effectiveness of modern therapeutic approaches is often limited by their toxic effects on other organs. In addition, many people in the world have limited or unequal access to cancer treatment services. Therefore, utilizing information from traditional medicine systems to identify alternative methods to prevent and control cancer. The use of traditional medicine can lead to the discovery of new bioactive compounds and drugs that are available, safe and affordable. In Islamic medicine, cancer is known as a disease with many treatment options. Traditional Islamic medicine suggests several strategies for cancer control and treatment. Surgical manipulation, venesection, dietary adjustment, and use of natural medicines including solid, semisolid, and liquid dosage forms by oral and external routes of administration are among these methods. This literature study will presents cancer from the perspective of traditional Islamic medicine, its aetiology and management.
The Effects of Sambiloto Leaf Extract (Andrographis paniculata) on Blood Sugar Regulation: An In Vivo Study Rachmat Hidayat; Lusia Hayati
Eureka Herba Indonesia Vol. 1 No. 1 (2020): Eureka Herba Indonesia
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ehi.v1i1.1

Abstract

Sambiloto (Andrographis paniculata) is one of the most common plants in Indonesia. Sambiloto contains quite varied secondary metabolites, where this plant is rich in flavonoids, alkaloids, terpenes and glycosides. This study aims to assess the effect of Sambiloto (Andrographis paniculata) leaf extract on blood sugar levels and the expression of GLUT4 protein in muscle tissue which indicates the potential of the test extract's ability to improve blood glucose intake to cells so that it can maintain blood sugar regulation. The process of extracting sambiloto is carried out by maceration in which 500 grams of simplicia are macerated with 96% ethanol for 72 hours. After 1 week of adaptation, the mice were randomly divided into the following six groups, each containing 5 animals: Normal control group, diabetes group (negative control), diabetes + metformin group (Met; 45 mg/kg), Diabetes + ES group (50 mg/kg), diabetes + ES group (100 mg/kg) and diabetes + ES group (200 mg/kg). The treatment with sambiloto extract was able to reduce blood sugar levels significantly, were at the ES 100 and 200 mg/kg BW doses it was able to reduce blood sugar levels to reach the target below 200 mg/kg BW. The dominant flavonoids in Sambiloto leaf extract are believed to be responsible for the effect of blood glucose regulation. In conclusion, Sambiloto extract affects lowering blood sugar levels in diabetes mellitus white rats by increasing glucose intake in cells and tissues.
Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in Rat Muscle Tissue Induced Aloxan Lusia Hayati; Rachmat Hidayat
Eureka Herba Indonesia Vol. 1 No. 1 (2020): Eureka Herba Indonesia
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ehi.v1i1.4

Abstract

Diabetes mellitus is a chronic condition that disturbs the body's blood sugar regulation. Bay leaves contain entirely various secondary metabolites, and this plant is rich in flavonoids, alkaloids, terpenes, and glycosides. This study aims to assess the effect of bay leaf extract (Syzygium polyanthum) on blood sugar levels and the expression of GLUT4 protein in muscle tissue. A total of 30 white rats (Rattus norvegicus) Wistar strains were obtained from the Eureka Research Laboratory (Palembang, Indonesia) weighing between 200 – and 250 grams. Bay leaf Simplicia was obtained from the Tawangmangu Herbal Research Center, Karanganyar, Indonesia. After 1 week of adaptation, the mice were randomly divided into the following six groups, each containing 5 animals: Normal control group, diabetes group (negative control), diabetes + metformin group (Met; 45 mg / kg), Diabetes + BLE (75 mg/kg), diabetes + BLE group (150 mg / kg) and diabetes + BLE group (300 mg/kg). Alloxan-induced white rats showed a very significant increase in blood sugar levels, where the use of the drug metformin was able to reduce blood sugar levels significantly even though they had not reached the target blood glucose target of less than 200 mg / dL. The treatment with bay leaf extract was able to reduce blood sugar levels significantly. The administration of metformin drugs or bay leaf extract showed the ability to increase the level of GLUT4 protein. In conclusion, bay leaf extract affects reducing blood sugar levels in diabetes mellitus white rats by increasing glucose intake in cells and tissues.
The Effect of Bay Leaf Extract (Syzygium polianthum) Against Cholesterol Regulation in Hypercholesterolemic Rats Lusia Hayati; Rachmat Hidayat
Eureka Herba Indonesia Vol. 1 No. 2 (2020): Eureka Herba Indonesia
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ehi.v1i2.6

Abstract

Cholesterol is an important precursor that plays a role in the production and regulation of various sterol group compounds, especially steroid hormone compounds, androgen hormone compounds, cortisol compounds, and estrogen compounds. This herb is known to have the effect of improving glucose regulation by increasing glucose intake into cells and tissues. Its ability to improve cell and tissue metabolism is mediated by the content of secondary metabolite compounds. This ability is believed to have the potential to improve cholesterol regulation. Bay leaf extract is effective in lowering cholesterol levels through regulation of Acetyl CoA production.
Co-Authors Aan Hardiyansah Abdul Mahatir Najar Abdulwahab Hakimi Abu Bakar Abu Bakar Abudarda, Andi Risda Fitrianti Achmar Mallawa Adnindya, Msy Rulan Agus Indra Jaya Agus Junaidi Agus Junaidi Agustian, Kresnawidiansyah Ahadi Aulia Rahman Ahmad Ahmad Ahmad Fatoni Ahmad Salim, Ahmad ahmad yani Ahmad Zain Sarnoto Ahmad, Arfandi Aji, Muhammad Pramono Alhan, Khusni Alvian, M. Andri Anang Tribowo Andayani, Nurfitri Andi Alfa Reza Andiani, Lia Andres Andres Andriana, Nadila Andronias Siregar Annisa Wimaulia Azlin Anuar, Arman Bin Aprilyanti, Cindy Apriwandi . Arifin, Nurdin Arindi Maretzka Aryanto, Hermawan Noval Ayeshah A Rosdah Azakhia, Yolanda Citra Baharuddin Baharuddin Bainamus, Putri Milanda Bella Stevanny Bemba, Tonia Buchari Buchari Budi Pratiti Burhan, Ahmad Syarif Carla R Marchira Catherine Christine, Debbie Cinthya Callathea Dahlan, Dadang - Dariyanto, Erwin David Usman Debby Handayanti Harahap Dessa, Rima Djoko Widodo Edith Humries edy susanto Ella Amalia, Ella Ellya Verawati Elsa Iskandar Erizka Rivani Erlina Erlina Erma Agusliani Ester G Pansserga Fahcreza Faisal Syahputra, Faisal Fajarwati, Lia Fathonah, Andina Nur Fatmawati Fatmawati Fatmawati Fildzah Hashifah Taufiq Fiona Widyasari Firdaus Muhammad Firjatullah, Andhika Fauziana Griselda Griselda Hadi, Dudi Abdul hadi, marwan Hammy, Hammy Hamzah, Fajri Handayani, Lisa Hani Subakti Haryanto Haryanto Herman Paninggiran Hermansyah Hermansyah Hermansyah Herry Hasnawi Herwansyah HUSNUL KHOTIMAH Ichsan, Amirul Ika Kartika Ikrima Ikrima Ilahi, Rahmat Imam Pribadi Inggarsih, Rara Irawan Sastradinata Irawan Sastradinata Irham Irham Irsan Saleh Ismar Hamid Iwan Setiawan Jamalullail Jayawarsa, A.A. Ketut Joko Marwoto Joko Marwoto Julian Sigap Rosadi Suwarso Junoretta Haviva Ernanto Kahar, Irsan Kahar, Irsan Kahar Kashardi, Kashardi Kemas Abdul Mutholib Luthfi Kemas Ya'kub Rahadiyanto Krishna, Hidan Bayu Krisna Murti Kumba Digdowiseiso, Kumba KURNIA KURNIA, KURNIA Lestari Barus, Irene Sukma Linda Purnama Listianingsih, Lona Lusia Hayati Lusia Hayati Lusia Hayati Lusia Hayati Lusia Hayati, Lusia Luthfi, Kemas Abdul Mutholib Mahmuddin Mahmuddin Mail, Abdul Maisya, Irin Makbruri Amin Malik, Aulia Januar Maria Margareth Wilson Marlinda, Dwi Martino Dwi Nugroho MARYANTI, LILIS Maslukin, Maslukin Mauladan, Ravy Maulana Saputra Maximillian Wilson Meilinda, Salsa Rizkia Mgs Irsan Saleh Mgs Irsan Saleh Mirna Dianita Muallin, Muallin Mubarok, E. Saefuddin Muhammad Anzarach Pratama Muhammad Bima Muhammad Damar Udin Muhammad Irsan Saleh Muhammad Reagan Muhammad Reagen Muhammad Ridwan Muhammad Rifani, Muhammad Muhammad Rizky Hidayatullah Muhlis Muhlis Mukti Zainuddin Murlena, Murlena Muslina Muslina Muthia, Putri Muthia, Putri Nadia Khoirina Najla Khairunnisa Nandang Iriadi Nani Solihati Nasriandi Nasriandi Ninuk Riesmiyantiningtias Nita Parisa Noorzaman, Sandi Noviyan, Rizky Dwi Nur Ainun Nur Riviati, Nur Nur Saqinah Galugu Nur, Rahmat Nur, Suaib Nurdina, Mutia Nurhasanah Nurhayati, M Sinta nurliah, nurliah Nurpahni, Siti Salma Nurrahmah, Andi Nurwijayanti Oktaviyani, Hikmatul Olivia Azalia Putri Pajarianto, Hadi Pamungkas, K J paosan, ahmad Patricia Wulandari Patry, Him Petty Purwanita Poku, Ham pongtiku, ita Prakarsa, Bambang Pramono, Yacob Agung Prayudhi, Risa Prihatina Anjela Purnamasari, Septi Puspa Sari, Puspa Puspa Zulaika Puspita, Sabrina Diah Puspitasari, Andini Putra, Suadi Sapta Putri Muthia Rachman, Andry Arifian Rachmat Hidayat Raden Ayu Adelia Safitri Raden Sunita Radiyati Umi Partan Radjikan Rahayu, Rosi Rahman, Ahadi Aulia Rahman, Ahadi Aulia Rahman, Alfi Rahmat Rais Rahmat Saleh Raihan Aqiilah N.L. , Andi Ahmad Ramadana, Radiyah Ramadhan, Hamzah Ramadhani, Anjas Ramli, Musdalifah Rara Inggarsih Rara Inggarsih Rashidah Unaib Al Zayid Rasyidah Jalil Reisha Notonegoro Resva Meinisasti Rety, Alghazali Riani Erna Rijaluddin, Khalid Rima Rachmawati Riri Juliantika Risnanosanti Risnanosanti Riswanto, A Heri Riswanto, A. Heri Riswanto, Andi Heri Riswanto, Heri Rosiyadi, Didi Safi'i, Imam Sahidan Sahidan Sahidan Sahidan Sahni Putri, Andi Rani Saksitha, Deassy Arestya Salni Salni Saman, Saman Samsuri, Fhadil Hidayat Sapinah, Pipin Sarah Sari , Windy Dian Sari H. S., Maharani Puspita Sari, Anggi Psutpita Sari, Windy Dian Sarmila, Sarmila Sarnato, Ahmad Zain Satriansyah , Arief Sekarini, Ratih Ayu Sepang, Rosa Marten Septi Purnamasari Septi Purnamasari SEPTIAN, DIAN Seruni, Seruni Shalita Dastamuar Shina Abdulkadir Sony Sanjaya Sonya Syarifah Sri Nita Sri Nita Sri Nita Sri Nita, Sri Sri Tuti Rahmawati Sri Vandayuli Riorini Sry Agustina St. Aisjah Farhum, St. Aisjah Sudaramanto, Bagus Sujud, Atsari Surya Ade Saputera Surya Darma Surya Darma Syarif Burhan, Ahmad Tanjung, Enha Syahrul Taufiq Indera Jayadi Tiara Fatrin Triwani Triwani Triwani Tungki Pratama Umar Tungki Pratama Umar Tunru, Andi Alif Udi Rusadi Uswatun Hasanah Veithzal Rivai Zainal Wahyudin Wahyudin Wardani, M. Fikri Wardiansyah Wiganepdo, Suryatno Wijaya, Maya Septa Winda Ramadianti, Winda Wirna, Risalah Sabila Yeni Agustin Yuliansyah, Chandra Yundari, Yundari Yuni sahruni, Ade Yusnaini Yusnia Budiarti Yusril Yusril Zaidan, Naufal Muhamad Zakaria, Khairunnisa Zen, Agustian Ziske Maritska