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CANNIBALISM PERFORMANCE OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) FED RATION SUPPLEMENTED WITH DIFFERENT DOSES OF 17α-METHYLTESTOSTERONE Heltonika, Benny; Sudrajat, Agus Oman; Junior, Muhammad Zairin; Widanarni, Widanarni; Suprayudi, Muhammad Agus; Manalu, Wasmen; Hadiroseyani, Yani
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.27-35

Abstract

One of the obstacles in Asian redtail catfish hatchery is the high cannibalism incidence. Cannibalism is associated with aggressive behavior caused by hormonal metabolism especially of testosterone. The purpose of this study was to evaluate the effect of testosterone administration on the cannibalism incidence in the Asian redtail catfish juveniles.  The experiment was conducted by rearing fish with initial length of 4.09±0.19 cm in 16 of 20 L aquariums with a density of 2 fish L-1. The experiment was designed with a completely randomized design with 4 treatments which were different level of 17α-methyltestosterone supplementation in feed, i.e., 0 mg kg-1 feed (A) as control, 7.5 mg kg-1 feed (B), 15 mg kg-1 feed (C), and 30 mg kg-1 feed (D). Each treatment has 4 replications. Fish fed experimental diet (40% protein) 4 times a day to satiation for 30 days. The parameters observed were type and index of cannibalism, aggressive behavior, survival rate, normal mortality, growth performance, hormones concentrations (estradiol, testosterone, and cortisol), and water quality. The results showed that cannibalism type II (the fish eaten completely or missing) and cannibalism index increased with the increasing doses of testosterone administration in the feed with the highest cannibalism incidence was 40.63%. The highest survival rate was found in treatment B (73.75±2.50%) and was not significantly different from the control treatment (69.38±2.39%). No differences in testosterone concentration and in the growth performance among the treatments.  However, there was a trend of decrease in the estradiol concentration of Asian redtail catfish juveniles fed ration supplemented with the increasing doses of 17α-methyltestosterone. Based on the results obtained in this research, estradiol changed in the body's plasma, it appears that there was a role for plasma estradiol concentration in controlling cannibalism of Asian redtail catfish juveniles. It concluded that the testosterone administration affected the cannibalism incidence in the Asian redtail Catfish. 
Studi Kasus Gallbladder Mucocele disertai Ehrlichiosis pada Anjing Beagle Deni Noviana; Jihan Fadilah Rachman Nurullah; Fitria Senja Murtiningrum; Dwi Utari Rahmiati; Wasmen Manalu
Acta VETERINARIA Indonesiana Vol. 11 No. 3 (2023): November 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.3.250-260

Abstract

Komplikasi gallbladder mucocele dan Ehrlichiosis akibat infeksi bakteri Ehrlichia canis (E. canis) dapat menyebabkan gangguan kesehatan hingga kematian pada anjing. Penelitian ini bertujuan mengetahui karakteristik gallbladder mucocele serta Ehrlichiosis pada anjing melalui interpretasi ultrasonografi abdomen, hematologi, dan kimia darah serta studi terkait terapi yang diberikan. Seekor anjing beagle betina berumur ±15 tahun milik RSHP SKHB IPB University memiliki keluhan muntah frekuen, penurunan nafsu makan, dan lesu. Pemeriksaan fisik pada hewan menunjukkan BCS rendah dan dehidrasi. Sonogram abdomen menunjukkan adanya pembesaran gallbladder disertai adanya endapan sekitar 90 % dengan ekhogenitas hiperekoik. Pemeriksaan darah menunjukkan peningkatan nilai ALT 132 U/L, total protein 8.3 g/dL, anemia (eritrosit 2.68 x 106 µL), trombositopenia (trombosit 20 x 103 µL), leukopenia (leukosit 4.8 x 103 µL), limfopenia (limfosit 0.6 x 103), dan kadar blood urea nitrogen yang tinggi (187 mg/dL). Evaluasi Rapid test kit menunjukkan anjing positif terinfeksi E. Canis. Kombinasi terapi asam ursodeoxycholic, hepatoprotektan, antibiotik, suplemen darah, dan vitamin diberikan selama 2 bulan. Evaluasi sonogram dan darah dilakukan setiap 4 minggu. Kondisi anjing sempat menunjukkan adanya perbaikan, namun akhirnya pasien mati setelah 8 minggu pengobatan.
Respons Fisiologis dan Gambaran Termografi Inframerah Kambing Sapera Induk Kondisi Bunting Pamungkas, Fitra Aji; Purwanto, Bagus Priyo; Manalu, Wasmen; Yani, Ahmad; Sianturi, Riasari Gail
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 8, No 3 (2021): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (44.813 KB) | DOI: 10.33772/jitro.v8i3.16436

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui respons fisiologis dan gambaran hasil penginderaan termografi inframerah dalam evaluasi kemampuan adaptasi kambing sapera induk kondisi bunting. Sebanyak empat ekor kambing perah sapera induk kondisi bunting dengan kisaran bobot badan 35-40 kg digunakan dalam penelitian ini. Parameter respons fisiologis yang diamati meliputi suhu permukaan kulit, suhu rektal, suhu tubuh, denyut jantung, dan frekuensi pernapasan, sedangkan gambaran termografi inframerah (IRT) dilakukan pada beberapa bagian tubuh, yaitu area mata, hidung, kaki, badan,  ambing, vagina, dan anal. Pengamatan respons fisiologis dan termografi inframerah dilakukan mulai pukul 06.00-18.00 WIB dengan selang waktu pengukuran setiap 2 jam. Data yang terkumpul dianalisis statistik secara deskriptif menggunakan SAS. Hasil penelitian menunjukkan bahwa respons fisiologis dan hasil penginderaan IRT pada kambing sapera induk kondisi bunting menunjukkan pola ritme harian atau sirkadian di sepanjang hari pengamatan. Kambing sapera induk kondisi bunting memiliki kemampuan adaptasi yang cukup baik, hal ini ditunjukkan dengan nilai parameter respons fisiologis maupun hasil penginderaan IRT yang masih berada dalam kondisi normal.Kata kunci: parameter fisiologis, termografi, inframerah, kambing Physiological Response and Infrared Thermographic Features Sapera Dairy Goat in Pregnant ConditioABSTRACT This study aimed to determine the physiological response and the description of infrared thermography sensing results in evaluating the adaptability of sapera dairy goat in pregnant conditions. A total of four sapera dairy goats in pregnant conditions with a body weight range of 35-40 kg were used in this study. Physiological response parameters observed included skin surface temperature, rectal temperature, body temperature, heart rate, and respiratory rate, while infrared thermography (IRT) images were performed on several parts of the body, namely the eye area, nose, feet, body, udder, vagina, and anal. Observation of physiological responses and infrared thermography was carried out from 06.00-18.00 WIB with a measurement time interval every 2 hours. The collected data were analyzed statistically descriptively using SAS. The results showed that the physiological responses and results of IRT sensing in pregnant sapera dairy goats showed a daily or circadian rhythm pattern throughout the day of observation. Sapera dairy goat in pregnant conditions have a fairly good adaptability, this is indicated by the value of the physiological response parameters and IRT sensing results that are still in normal conditions.Keywords: physiological parameters, thermography, infrared, goat
Tingkat Aktivitas Sel Endokrin Penghasil Folikel Stimulating Hormon (FSH) Terkait Pemberian Pasak Bumi (Eurycoma Longifolia JACK) Pratomo, Hurip; Supriatna, Iman; Winarto, Adi; Manalu, Wasmen
Jurnal Kefarmasian Indonesia VOLUME 2, NOMOR 1, FEBRUARI 2012
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v2i1.3715

Abstract

Pasak bumi is popular as an aphrodisiac. Traditionally, pasak bumi infusion is used to increase libido and duration of erection. The research was focused on: Activity level of FSH cells after three days of pasak bumi treatment. The research was carried out by evaluation using immunohistochemistry method via antibody anti FSH staining. The result of the research showed that a dosage of 18 mg/200 g body weight (bw) orally of pasak bumi maintained certain endoceine positive cells e.g. FSH production cells and were found stabile in the third days. Pasak bumi serves a stabilizer for intracellular levels of FSH in the anterior hipophysis/pituatary.
The Potential Uses of Curcumin and PMSG Hormones to Increase Egg Production of Muscovy Ducks through Increasing Estradiol Concentrations and Shortening Laying Rest Period Dwi Gunadi; Rukmiasih Rukmiasih; Wasmen Manalu
ANIMAL PRODUCTION Vol. 23 No. 2 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.2.98

Abstract

The aim of this research was to study the effects of curcumin administration, injection of PMSG hormone, and the combination of curcumin supplementation and PMSG injection on the physiological parameters and performance of female Muscovy ducks. The objects of this research were 40 young female Muscovy ducks, aged 24 weeks, subjected to treatments: control (P1), curcumin supplementation at a dose of 24 mg/100 g feed (P2), intramuscular injection of PMSG hormone at a dose of 0.015 mL/hen (P3), and the combination of curcumin and PMSG (P4). The results showed that the treatment gave nonsignificant improvements in Muscovy duck performances, including feed consumption, egg production, and the day of laying period and molting period. Treatment gave a significant increase in serum estradiol concentration at molting period after treatment. This study concluded that the treatment provides no significant improvement in the performance of female Muscovy ducks because the genetic diversity of Indonesian ducks remains high.
Iklim Mikro dan Respon Fisiologis Sapi Pesisir di Dataran Rendah dan Dataran Tinggi Sumatera Barat Yetmaneli Yetmaneli; B. P. Purwanto; Rudi Priyanto; Wasmen Manalu
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16017

Abstract

ABSTRAK. Penelitian bertujuan melihat potensi iklim mikro dan respon fisiologis sapi Pesisir yang dipelihara di dataran rendah (Kota Padang (0-300 m dpl )) dan dataran tinggi (BPTU Padang Mengatas ( 600 m dpl)) Sumatera Barat. Materi yang digunakan dalam penelitian ini 8 ekor sapi Pesisir, variabel yang diukur terdiri dari 2 aspek yaitu lingkungan abiotik dan aspek fisiologis sapi Pesisir. Aspek lingkungan abiotik berupa suhu lingkungan (Ta), kelembapan udara (RH) serta Temperature Humidity Index (THI). Variabel fisiologis sapi meliputi suhu rektal (Tr), suhu kulit (Ts), frekuensi pernapasan (RR) dan denyut jantung (HR). Tr dan TS digunakan menghitung suhu tubuh sapi (Tb). Tr dan RR digunakan menghitung Heat Tolerance Coefficient (HTC). Uji beda (t-test) digunakan untuk mengetahui ada tidaknya perbedaan kondisi iklim dan respon fisiologis sapi Pesisir di dataran rendah dan tinggi Sumatera Barat. Hasil penelitian menunjukkan potensi iklim di dataran rendah adalah rerata suhu lingkungan 29,96C, rerata kelembapan 64,22%, rerata THI 79,96 sedangkan potensi iklim di dataran tinggi adalah rerata suhu lingkungan 25,42C, rerata kelembapan 69,48%, rerata THI 74,3. Rerata daya tahan panas sapi di dataran rendah 1,78 dan dataran tinggi 1,82. Kesimpulan penelitian menunjukkan potensi iklim dataran rendah untuk pemeliharaan sapi termasuk zona cekaman panas sedangkan dataran tinggi dalam cekaman ringan. Kondisi fisiologis sapi Pesisir di dataran rendah yang berbeda dengan sapi yang di dataran tinggi adalah suhu rektal, suhu kulit, suhu tubuh dan denyut jantung sedangkan frekuensi pernapasan didapatkan sama di kedua dataran. Daya tahan panas sapi Pesisir cukup baik ditemui di kedua dataran Sumatera Barat.(Microclimate and physiological responses of Pesisir cattle at lowland and highland of West Sumatra) ABSTRACT. This research aimed to investigate the potential of microclimate and physiological responses of Pesisir cattle that are maintained in lowland (Padang City (0-300 m asl)) and highland (Padang Mengatas BPTU ( 600 m asl)) in West Sumatra. The experimental animals were 8 pesisir cattle. The measured variables were abiotic environment and physiological responses of Pesisir cattle. Abiotic environmental measures were ambient temperature (Ta), humidity (RH) and Temperature Humidity Index (THI). Physiological variables were rectal temperature (Tr), skin temperature (Ts), respiratory rate (RR), and heart rate (HR). Tr and Ts were used to determine body temperature (Tb). Tr and RR are variables for calculating Heat Tolerance Coefficient (HTC). To determine whether there are differences in climatic conditions and physiological values in the two regions, the data were analyzed using a t-test. The results showed that climate potential in the lowlands was the average Ta 29.96 C, the average Rh 64.22% with an average THI 79.96 while the potential climate in the highlands was the average Ta 25.42C, the average Rh 69, 48% with a mean THI of 74.3. The average HTC of cattle in the lowlands is 1.78 and the highlands is 1.82. The conclusion showed the climate potential of lowland for raising beef cattle includes heat stress zones, while the highlands there was mild stress. The physiological conditions of Pesisir cattle in the lowlands different from the highlands are Tr, Ts, Tb, and HR while RR is found the same in both plains. HTC of Pesisir cattle is good in both plains of West Sumatera.
Respon Fisiologis dan Penyusutan Bobot Badan Domba Lokal Jantan terhadap Transportasi dengan Posisi Berbeda dalam Kendaraan Lendrawati Lendrawati; Rudi Priyanto; Mohamad Yamin; Anuraga Jayanegara; Wasmen Manalu; Desrial Desrial
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14877

Abstract

ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi respon fisiologis dan penyusutan bobot badan domba lokal jantan terhadap transportasi dengan posisi yang berbeda pada kendaraan. Penelitian ini menggunakan 10 ekor domba lokal jantan yang berumur 8-10 bulan dengan rataan bobot badan 16,11 2,27 kg. Penelitian ini menggunkan Rancangan Acak Lengkap dua faktor dengan 5 ulangan. Faktor pertama adalah: posisi domba di kendaraan terdiri dari: posisi berdiri dan berbaring. Faktor kedua adalah waktu pengambilan sampel yaitu: sebelum dan setelah transportasi. Semua domba perlakuan diangkut dalam satu mobil Pick up selama 8 jam perjalanan dengan tingkat kepadatan yang sama yaitu 0,27 m2/ekor. Data bobot badan, suhu rektal, laju respirasi, denyut nadi, hormon kortisol, glukosa, kreatinin, hemoglobin dan hematokrit dikumpulkan sebelum dan setelah transportasi. Hasil penelitian menunjukkan bahwa tidak terdapat interaksi yang nyata (P0,05) antara posisi domba dikendaraan dengan waktu pengambilan sampel pada semua parameter (kecuali denyut nadi). Penempatan domba dengan posisi berdiri dan berbaring selama 8 jam transportasi memperlihatkan respon yang sama terhadap penyusutan bobot badan, suhu rektal, laju pernapasan, hormon kortisol, konsentrasi glukosa, kreatinin, hemoglobin dan hematokrit. Kesimpulan penelitian ini adalah penempatan dengan posisi berdiri dan berbaring di kendaraan selama 8 jam transportasi memperlihatkan respon fisiologis dan penyusutan bobot badan yang sama pada domba lokal jantan.(Physiological responses and body weight loss of male local sheep during transportation with different position on the vehicle)ABSTRACT. The aim of this study was to evaluate the physiological responses and body weight loss of males local sheep to transportation with different positions on the vehicle. Ten males sheep with 16.112.27 kg of body weight and 8-10 months in aged were used in this study. Completely Randomized Design with two factors was used in this study. The first factor was positions on the vehicle (standing and lying down) and the second factors was sampling times (before and after transportation). All of sheep were transported in one Pick up (Mitsubishi L 300) for 8 hours with similar of density level of 0,27 m2/head. Data on body weight, rectal temperature, respiration rate, pulse, cortisol, glucose, creatinine, hemoglobin and hematocrit were collected before and after transportation. Results showed there was no significant interaction (P 0.05) between the position of the sheep in the vehicle with the time of sampling in all parameters (except pulse rate). Placing of sheep with standing and lying down position for 8 hours of transportation showed the same responses for body weight loss, rectal temperature, respiratory rate, cortisol level, glucose concentration, creatinine, hemoglobin and hematocrit. Based on those findings, it can be concluded that standing and lying position in the vehicle for 8 hours of transportation shows the similar effect on the physiological responses and body weight loss of local male sheeps.
Traumatic toenail wound in a Thai elephant (Elephas maximus indicus) : Toenail wound in elephant Lynette Ong Huey; Agik Suprayogi; Aryani Sismin Satyaningtijas; Damiana Rita Ekastuti; Hera Maheshwari; Isdoni Bustaman; Koekoeh Santoso; Pudji Achmadi; Wasmen Manalu; Sattabongkoch Maneewong; Ronald Tarigan
ARSHI Veterinary Letters Vol. 8 No. 1 (2024): ARSHI Veterinary Letters - February 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.1.3-4

Abstract

Elephant is Thailand’s national animal which represents three aspects: loyalty, longevity, and strength. Thai elephants are often used for deforestation and tourist activities, such as elephants, riding, and showering. This case report highlights a traumatic wound in the left foreleg nail of a showering Thai elephant. Nail wounds undergo infection and tissue necrosis. A bone fragment suspected to be part of the distal phalanges was found and confirmed by X-ray examination. However, a surgical approach is not recommended because of the high risk of sedation in elephants. Routine wound cleaning with antiseptics and topical antibiotics was performed on the traumatic wounds. The bone fragment was removed after necrosis of the surrounding tissues. The prognosis for this case was considered good based on the location and wound condition.
Daya tahan panas berbagai bangsa sapi pedaging di peternakan rakyat Kampung Astomulyo, Kabupaten Lampung Tengah Irfan Nurhidayat; Agik Suprayogi; Aryani Sismin Satyaningtijas; Damiana Rita Ekastuti; Hera Maheshwari; Isdoni Bustamam; Koekoeh Santoso; Pudji Achmadi; Wasmen Manalu; Ronald Tarigan
ARSHI Veterinary Letters Vol. 8 No. 1 (2024): ARSHI Veterinary Letters - February 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.1.17-18

Abstract

Animals' productivity and health status in tropical regions are significantly influenced by their ability to adapt to hot and humid tropical environments. This study aimed to compare the heat tolerance of the Ongole-cross (OC), Simmental-cross (SC), and Limousin-cross (LC) breeds raised in Astomulyo Village, Lampung Tengah District. This location had a high environmental stress index in the afternoon and moderate environmental stress in the morning and evening. Most cattle have a Heat Tolerance Coefficient (HTC) above 100, and there are no significant differences in HTC values among cattle breeds. The OC, SC, and LC cattle breeds exhibit good heat tolerance and can adapt to hot and humid environments in Lampung Tengah District.
Pengujian Toksisitas Akut Lethal Dose 50 (LD50) Infusa Ikan Nomei (Harpodon nehereus) dengan Menggunakan Mencit (Mus musculus) sebagai Hewan Uji yuri, Nurasmi yuri; I Ketut Mudite; Andriyanto; Wasmen Manalu
Acta VETERINARIA Indonesiana Vol. 12 No. 3 (2024): November 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.3.187-193

Abstract

Ikan Nomei (Harpodon nehereus) merupakan jenis ikan yang lembek dan memiliki nilai ekonomis yang cukup tinggi untuk dapat dimanfaatkan secara luas. Penelitian bertujuan menguji toksisitas akut ekstrak infusa daging ikan nomei Harpodon nehereus pada mencit betina dengan menentukan nilai lethal dose 50 (LD50), mengamati pengaruhnya pada organ tubuh mencit, dan menghitung konsentrasi ekstrak yang paling efektif. Hewan uji yang digunakan adalah mencit galur DDY sebanyak 25 ekor dibagi secara acak ke dalam 5 kelompok perlakuan, yaitu kelompok kontrol (pemberian akuades) dan kelompok perlakuan pemberian infusa nomei masing-masing dengan dosis 5, 10, 15, dan 20 g/kg BB secara per oral. Parameter yang diamati meliputi mortalitas, respons fisiologis, gejala klinis, bobot badan, bobot absolut organ, dan bobot relatif organ. Nilai LD50 menunjukkan pemberian infusa ikan nomei pada mencit betina bersifat tidak toksik. Pemberian infusa nomei sampai dengan dosis 20 g/kg BB tidak menimbulkan gejala klinis yang bersifat patologis, perubahan makroanatomi organ, atau kematian. Pemberian infusa tidak menunjukkan efek yang signifikan pada peningkatan atau penurunan bobot badan. Berdasarkan penelitian, pemberian infusa ikan nomei sampai dengan dosis 20 g/kg BB bersifat tidak menimbulkan kelainan baik secara klinis atau kelainan pada organ mencit. Kesimpulan dari penelitian ini adalah infusa ikan nomei termasuk dalam golongan bahan yang diklasifikasikan dalam sediaan praktis tidak toksik (aman).
Co-Authors - Hernawati . Safrida ., Chairul A Yani ABADI SUTISNA Abdul Zahid Adawiah . Ade Sunarma Ade Sunarma Adi Winanto Adi Winarto Adi Winarto Adi Winarto Agik Suprayogi Agus Oman Sudrajat Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Ahmad Subhan ahmad yani Ahmad Yani Aisjah Girindra Alfred O. M. Dima Alifiana Fitrianingrum Amrozi Andi Mu’nisa Andri Yanto Andriyanto Andriyanto Andriyanto Andriyanto . Andriyanto A Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Anita Esfandiari Anuraga Jayanegara Arief Boediono Aryani Satyaningtijas Aswani, Tuti Aulia Andi Mustik Aulia Andi Mustika Aulia Andri Mustika Awaludin Azam B. Zaidy B P Purwanto B. P. Purwanto Bagus Priyo Purwanto Bambang Gunadi Bambang Gunadi Bambang Gunadi Bambang Kiranadi BAMBANG KIRANADI Bambang Kiranadi Bambang Pontjo Priosoeryanto Benny Heltonika Bondan Achmadi Cece Sumantri Chairul - Chairul Chairul Chairun Nisa Christina Clarice Leksono Cut Dara Dewi Damiana Rita Ekastuti Debby Jacqualine Jochebed Rayer Dedy D. Solihin Deni Noviana Deni Radona, Deni Desrial DEWI APRI ASTUTI Dewi Ratih Agung Priyono Dewi Ratih Agungpriyono Dewi Ratih Agungpriyono Dewi Ratih Agungpriyono Diah Nugrahani Pristihadi Diky Yuliansah Diky Yuliansah Dwi Gunadi Dwi Setyaningsih Dwi Utari Rahmiati Dyah Iswantini Edwin Ligia Sastra Ekowati Handharyani Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Erli Chandra Etih Sudarnika EVY AYU ARIDA Evy Ayu Arida Fachriyan H. Pasaribu Firda Agustin Firda Agustin Firda Agustin, Firda Fitra Aji Pamungkas Fitra Aji Pamungkas Fitra Aji Pamungkas Fitria Senja Murtiningrum Friska Mery Montolalu Ganjar Maulana Nugraha HADI SUNARYO Hamdika Yendri Hamdika Yendri Hamdika Yendri Hamdika Yendri Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamny Sofyan Henni Syawal HERA MAHESHWARI Hera Maheswari, Hera Hernawati Hernawati Hesti Wahyuningsih Huda Shalahudin Darusman Hurip Pratomo Hurip Pratomo Husna, Hanifati I Ketut Mudite I Komang Gede Wiryawan Iman Hernaman Iman Supriatna Ira Agustina Dewi Gandasari Irfan Nurhidayat Irma Herawati Suparto Irzaman, Irzaman Isdoni Bustaman Jakaria Jakaria Jihan Fadilah Rachman Nurullah Jola J.M.R. Londok Joni Haryadi, Joni Kapelle, Imanuel B. D. Kardiyo Praptokardiyo Karin katina Hasya Kasiyati Kasiati Kharisma Mardathilah Kharisma Mardathilah Kiranadi Bambang koekoeh santoso Koekoeh Santoso Koekoeh Santoso Koeswinarning Sigit Komariah Komariah Komariah Krido Brahmo Putro Kusdiantoro Mohamad Kusumorini Nastiti La Eddy La Jumadin Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Widi Leliana Widi Lendrawati Lendrawati Leo Sapelani Soinbala Lies Parede Lina Noviyanti Sutardi Lis Rosmanah Lis Rosmanah Livana Dethris Rawung Lucia Johana Lambey Lynette Ong Huey M. Zairin Junior M.Y Sumaryadi Mas Yedi Sumaryadi Masriani . Meillisa Carlen Mainassy Mien Th. R. Lapian Mohamad Hasil Tamzil Mohamad Yamin Mohamad Yogie Hendrawan Mohammad Miftahurrohman Mokhamad Fakhrul Ulum, Mokhamad Muhaimin Hamzah MUHAMMAD AGUS SUPRAYUDI Muhammad Darjat Darulfalah Muhammad Junior Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Junior Murniaty Simorangkir Nabila Martha Ludi Miftahurahma Nahrowi Nahrowi Nastiti Kusomorini Nastiti Kusumorini NASTITI KUSUMORINI NASTITI KUSUMORINI Nastiti Kusumorini Nelda Fliza Zora Ni Made Ria Isriyanthi Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nurhidayat - Nurhidayat, Nurhidayat Odang Carman Pollung Hasiholan Siagian PONIMAN PONIMAN Pudji Achmadi Purwantiningsih Sugita Putut I. Pudjiono R G Sianturi Raden Roro Sri Pudji Sinarni Dewi Rahminiwati, Min Rarah Ratih Adjie Maheswari Resi Milna Revolson Mege Riasari Gail Sianturi Riasari Gail Sianturi Riasari Gail Sianturi Ridi Arif RIDWAN AFFANDI Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Risna Anggraeni Ronald Tarigan Ronald Tarigan Ronald Tarigan Ronny Rachman Noor Rosdianto, Aziiz Mardanarian Rosdianto, Aziiz Mardanarian Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih Ruqiah Ganda Putri Panjaitan Sabri Mustafa Safrida Safrida SATA YOSHIDA SRIE RAHAYU Sattabongkoch Maneewong Sela Septima Mariya Sharon Aurelia Sharon Aurelia Sharon Aurellia Silmy Kamila Widyanti Silmy Kamila Widyanti Sitti Wajizah Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih Sri Nuryati Sri Subekti Subangkit, Mawar Suhendro, Ikhsan Sumiati Sumiati Sumiati Sumiati Sumiaty Aiba Sunarno . Sunarno s Sunarno Sunarno Sutiastuti Wahyuwardani Sutiastuti Wahyuwardani SYAHRUN HAMDANI NASUTION TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Tiltje Andretha Ransaleleh TOHA SUTARDI Toto Toharmat Tri Budhi Murdiati Tri Budhi Murdiati Tuti Aswani Tutik Wresdiyati Umi Cahyaningsih Uus Saepuloh Wahyuni, Sri Wida Lesmanawati Widanarni Widanarni WIDANARNI WIDANARNI Wiranda G Piliang Wiranda G Piliang Wiranda G. Piliang Wiwin Winarsih Y. Hadiroseyani Yayuk Sri Rahayu Yetmaneli, Yetmaneli Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yulia Yellita Yulvian Sani yuri, Nurasmi yuri Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang Yusfiati Yusfiati Yuvensius Yuvensius Zulfa Zakiah