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Pemberian Pregnant Mare Serum Gonadotropin Sebelum Perkawinan dan Jamu Selama Kebuntingan untuk Memperbaiki Performa Anak Domba (ADMINISTRATION OF PREGNANT MARE SERUM GONADOTROPIN AND JAMU DURING GESTATION TO IMPROVE FOR LAMB PERFOMANCE) Andriyanto .; Ridi Arif; Adi Winarto; Leo Sapelani Soinbala; Bondan Achmadi; Aulia Andi Mustika; Diah Nugrahani Pristihadi; Amrozi .; Wasmen Manalu
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Administration of pregnant mare serum gonadotropin (PMSG) hormone prior to mating increasesquality of lamb born on one and two litter sizes, but in three litter size the motality is higher. Administrationof traditional herbal medicine “jamu” consisting of ginger,green chiretta, cinnamon, Zingiberzerumbet, andpepper during gestation increases performance of lambs. This research was conducted to explore theeffectivity of PMSG injection prior to mating and jamu administration during gestation to increase lambsperformance. Eighteen priangan fat-tail ewes with weight around 20-25 kg were injected with PGF2á at adose of 10 mg/ewe twice with 11 days interval to synchronize estrous cycle. Injection of PMSG at a dose of200 IU/ewe was conducted at the second PGF2á injection. The experimental ewes were mated naturally anddivided into a randomized design with a 2 x 3 factorial arrangement with three replications. The firstfactor was dose of PMSG with two levels i.e., 0 and 200 IU/ewe. The second factor was dose of jamu withthree levels i.e., 0, 15, and 30 mL/ewe. Jamu was administered orally every week during gestation. Injectionof PMSG and administration of jamu decreased prenatal mortality, increased ratio of lamb per ewe, increased total lamb born with average birth weight and total birth weight were higher in jamu at a doseof 15 and 30 mL/ewe by 30.02 and 31.76%, repectively. During the first month postnatal, lambs born toewes injected by PMSG and administered jamu had higher number of lambs survive, average weight, totalweight around 3 times as compared to control. It was concluded that injection of PMSG prior to mating andadministration of jamu during pregnancy increase lamb birth weight and improve the quality of lamb.
Patogenesitas Virus Gumboro Isolat Lokal pada Ayam Pedaging Sutiastuti Wahyuwardani; Dewi Ratih Agungpriyono; Lies Parede; Wasmen Manalu
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Study of the pathogenicity of gumboro virus (very virulent Infectious Bursal Disease virus, vvIBDV) oflocal isolates was performed in broilers. The chickens were grouped into four: i) infected with vvIBDV; ii)vaccinated with commercial vaccine + challenge with vvIBDV; iii) vaccinated using locally produced vaccine+ challenged with vvIBDV; and iv) control group (unvaccinated animals). Pathogenecity was analyzedbased on the sequence of infection; the distribution and degree of gross pathology and histopathologylesions on bursa Fabricius, spleen and thymus in relation to the presence of the antigen (IBDV Ag) whichwas detected using immunohistochemistry technique. During the acute phase (1-3 days following theexperimental infection) hyperemia and exudation on the bursa Fabricius were observed in the infectedgroup and the two vaccinated-challenged groups. Similarly, hyperemia was also observed on the spleenand thymus. Whilst during the chronic stages (at 7 and 14 days following the experimental infection) thebursa showed atrophy. Upon histological examination, the bursa showed interstitial edema, hemorrhage,infiltration of heterophyl cells and fibroblast hyperplasia. The bursa lymphoid follicles were depleted dueto cells necrosis and apoptosis. Plica epithelial layer was wrinkled and the Goblet cells metaplasia formedhyper plastic cysts. In the spleen and thymus severe reticulo endothelial cells proliferation was observedduring the acute stage and tended to decline during the chronic stage. During the acute and chronic stagescells containing IBDV Ag were detected in the bursa Fabricius, distributed within the mantle zone andgerminal center of lymphoid follicles in the two vaccinated-challenge groups. Whereas in the infectedgroup, the IBDV antigen were detected up to 7 days post the infection. Nevertheless, neither IBDV antigenwas detected in the spleen nor in the thymus in all the experimental groups. The results of this studyclearly explained the ferocity of vvIBDV of local isolates.
Pemberian Vaksin Ichthyophthirius multifiliis untuk Mencegah Ichthyophthiriasis pada Ikan Mas (APPLICATION OF ICHTHYOPHTHIRIUS MULTIFILIIS VACCINE FOR PREVENTION OF ICHTHYOPHTHIRIASIS IN COMMON CARP) Henni Syawal; Nastiti Kusumorini; Wasmen Manalu; Ridwan Affandi
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was conductedto assess the physiological responses of commoncarp due to administrationof ich vaccines and kept at different water temperatures. Completely Randomized Factorial Designwasusedin this study. The number and the size of the experimental fish was720 and 5-7 cm, respectively. Fish wasvaccinated by immersing the fish in water that contained 3 mLL-1 of ich vaccines for 15 minutes. Then, fishwas kept for 21 days at various temperatures: at 18ºC which was room temperature without heater andfish had not been vaccinated and at 20, 24, and 28ºC where fish was given the vaccines and the aquariumis facilitated with electric heater. Atday15post immunization, challenge test with 5.000 cells of life therontfish-1was performed.Immobilization test, differential leukocyte, prevalence rates, and survival rates weremeasured. The measurements of these parameters were performed four times:before the treatment (day0), day7, day14, and day21, respectively. The results showed that the vaccine could reduce the stress inthefish that were kept at 20, 24, and 28ºC, and the prevalence of ichthyophthiriasis. In addition the vaccineincrease the fish survival rates after challenge test. The highest survival rate (100%) was found in fish thatwerekept in 28ºC.
Keragaman Gen Heat Shock Protein 70 pada Ayam Kampung, Ayam Arab, dan Ayam Ras (POLYMORPHISMS OF THE HEAT SHOCK PROTEIN 70 GENE IN KAMPONG, ARABIC, AND COMMERCIAL CHICKENS) Mohamad Hasil Tamzil; Ronny Rachman Noor; Wasmen Manalu; Cece Sumantri
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the research was to identify the polymorphisms of HSP-70 gene of kampong, Arabic, andcommercial chickens. The study used whole blood samples taken from the branchial vein at the age of 12weeks from a group of chickens that were kept since DOC. Kampong chickens DOC were obtained byhatching the eggs that were collected from lowlands, medium and high altitude areas in Lombok island,while the Arabic chickens DOC were obtained from farmer. DOC of commercial chickens were purchased atthe poultry shop. The genotyping results using PCR-SSCP analysis showed that kampong and arabicchickens were polimorphic, while commercial chickens were  classified as monomorphic. Kampong chickenshad seven genotypes of HSP-70, namely AA, AB, AC, CC, AD, DD, and BC.  The AD genotypes had thehighest frequency, whereas in arabic chickens had six genotypes, namely AA, AB, AC, CC, AD and BC,which AC genotype had the highest frequencies.  However, the commercial chickens contain only onegenotype which was DD. There were three polymorphic sites found in amplificated area, namely AÆGmutation at site 617, AÆG mutation at site 628, GÆC mutation at site 646,  CÆT mutation at site 661,AÆG mutation at site 699, GÆC mutation at site 754, and AÆG mutation at site 792.  Mutations at sites628, 646 and 661 were considered as silent mutation. HSP-70 gene in kampung and arabic chickenpopulation in this study was in equilibrium. The value of Ho, He, and PIC of kampung and arabic chickenswere not different.
Gambaran Patologi Bursa Fabricius Embrio Ayam Pascavaksinasi Gumboro Secara In Ovo Menggunakan Vaksin Lokal dan Komersial (PATHOLOGIC DESCRIPTION OF BURSA FABRICIUS CHICKEN EMBYROS AFTER IN OVO VACCINATED WITH LOCAL AND COMMERSIAL GUMBORO VACCINES) Sutiastuti Wahyuwardani; Dewi Ratih Agung Priyono; Wasmen Manalu
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bursa Fabricius is a target organ of gumboro virus infection which is often damaged after vaccinationusing hot intermediate gumboro live vaccine. The purpose of this study was to assess pathologic effect oflocal and commercial gumboro vaccines . As many as 45 embryo chicken eggs at nine day old were used inthis research, then grouped into three groups of 15 embryos chicken eggs each, these were: Embryo chickeneggs without vaccination (Group I), vaccinated with IBD intermediate plus commercial vaccine (Group II)and IBD intermediate plus local vaccine (Group III). Vaccinations were done at 14 days old. All groups thenterminated each three embryos at 12 hours, 1, 2, 3 days post vaccination. The results showed that pathologicanatomic lesions could not be detected. Whereas pathologic lesions were detected in the group that werevaccinated with intermediate plus local IBD observed more severe than in the group that vaccinated withintermediate plus commercial IBD. Lesions such as edema, hemorrhages, necrosis of lymphoid cells wereobserved microscopically in embryo at 12 hours, 1, 2 and 3 days post vaccination in Group II and group III.The lesions were more severe at two days post vaccination causing some lymphoid follicles disappeared at three days post vaccination. However, they were not detected again in the bursa Fabricius three days afterhatching. Cells containing antigens of gumboro were detected in the bursa Fabricius of chicken embryo atone day until three days post vaccination, then disappeared after three days post hatch. It was concludedthat pathologic description of bursa fabricius showed that virus vaccines used for vaccinated IBD in ovowere still virulent, that can cause histopathologic lesions. The viruses are suggested to be more attenuatedbefore using as vaccine in ovo.
Peningkatan Produktivitas Domba pada Skala Peternakan Rakyat Melalui Pemberian Hormon Pregnant Mare Serum Gonadotrophin (IMPROVEMENT OF SMALL HOLDER FARMS SHEEP PRODUCTIVITY THROUGH THE APPLICATION OF PREGNANT MARE SERUM GONADOTROPHIN) Andriyanto .; Wasmen Manalu
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The administration of Pregnant Mare Serum Gonadotrophin (PMSG) hormone to stimulate superovulation has been proven to improved fetal prenatal growth, birth weight, mammary gland growth anddevelopment, milk production, litter size, as well as pre and post weaning growth. This study was conductedto apply the administration of PMSG hormone technology in small-holder farms. One hundred ewes weredivided into two groups. Group I (control: 50 ewes) ewes without administration of PMSG and Group 2(treatment: 50 ewes) ewes with administration of PMSG to stimulate super ovulation and improveendogenous secretion of pregnant hormones. The application of PMSG increased the numbers of lamb bornby 26.8% (69 vs. 87) with average litter size of 20.7% (1.38 vs. 1.74). The lamb weight at birth was alsoincreased by 25.7% (2.76 ± 0.33 vs. 3.47 ± 0.35 kg). The milk production of the dam was also increased by29.7% (0.64 ± 0.25 vs. 0.91 ± 0.22 L/ewe/day). Lambs born to ewes administered PMSG had better weaningweight 22.1% (12.32 ± 2.44 vs. 15.04 ± 1.11 kg/ewe). The number of survival lamb to reach weaning age washigher compared to the control group. The weight at weaning age was almost twice (628.4 kg vs. 1202.8 kg)compared to the control group. Using simple economic analysis calculation, the application of this hormonetechnology could increase gross revenue by Rp 294.280- per ewes. It was concluded that the administrationof PMSG is economically feasible to be applied in to the small-holder farms.
KUALITAS DAN PERFORMA TELUR IKAN LELE (Clarias gariepinus ) YANG DIHASILKAN OLEH INDUK DENGAN SUPLEMENTASI KURKUMIN DAN HORMON TIROKSIN (Quality and Performance of Egg from African catfish (clarias gariepinus) Broodstock Supplemented with Curcumin) Livana Dethris Rawung; Damiana Rita Ekastuti; Ade Sunarma; Muhammad Zairin Junior; Min Rahminiwati; Wasmen Manalu
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 16, No 4 (2020): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.16.4.243-249

Abstract

Permasalahan utama dalam kegiatan usaha budidaya perikanan antara lain adalah ketersediaan benih ikan yang tepat waktu, tepat jumlah dan berkualitas. Ketersediaan benih ikan sangat tergantung pada jumlah telur yang dapat dibuahi dan ditetaskan. Semakin tinggi presentase pembuahan dan penetasan maka akan semakin tinggi pula jumlah larva yang dapat dihasilkan. Penelitian ini menggunakan ikan lele sebagai hewan coba dengan tujuan untuk mengetahui pengaruh suplementasi kurkumin dan tiroksin pada induk ikan lele terhadap kualitas dan performa telur yang dihasilkan. Suplematasi kurkumin dan hormon tiroksin melalui pakan selama 12 minggu pemeliharaan. Parameter yang dianalisis berupa konsentrasi vitelogenin telur, diameter telur, koefisien keragaman diameter telur, konsentrasi lipid dalam telur (triglserida, kolesterol dan HDL), derajat pembuahan dan derajat penetasan. Hasil penelitian menunjukkan bahwa kelompok perlakuan yang diberikan kombinasi penambahan kurkumin dan hormon tiroksin memiliki nilai konsentrasi vitelogenin (8.17+2.74 µg/mL) (p<0,05), diameter telur (1.43+0.00 mm) (p<0,05), dan konsentrasi trigliserida (4.89+0.53 mg/g) (p<0,05) tertinggi diantara semua perlakuan, sementara itu kelompok perlakuan yang baik hanya diberikan penambahan kurkumin, maupun kombinasi penambahan kurkumin dan tiroksin cenderung memiliki nilai derajat pembuahan dan penetasan yang lebih tinggi dibandingkan dengan kelompok perlakuan yang tidak diberikan penambahan kurkumin.  The main problem in aquaculture is the availability of fish seedlings on time, in the right quantity and quality. The availability of fish seeds is determined by the number of eggs that can be fertilized and hatched. The higher the percentage of fertilization and hatching, the higher the number of larvae that can be produced. This study uses catfish as experimental animals because catfish are one of the most consumed cultivation commodities. The aim of this study is to determine the effect of curcumin and thyroxine supplementation on catfish brooders on the quality and performance of eggs produced. Catfish used in this experiment were given the addition of curcumin and thyroxine hormone through the feed for 12 weeks of rearing. Parameters analyzed were egg vitellogenin concentration, egg diameter, coefficient of diversity in egg diameter, lipid concentration in eggs (triglycerides, cholesterol, and HDL), degree of fertilization and degree of hatching. The results showed that the group given the combination of the supplementation curcumin and the thyroxine hormone had the values of vitellogenin concentration (8.17+2.74 µg/mL) (p <0.05), egg diameter (1.43+0.00 mm) (p <0.05), and triglyceride concentration (4.89+0.53 mg/g) (p <0.05) the highest among all treatments, meanwhile the group is only given the addition of curcumin, and the combination of supplementation of curcumin and thyroxine tends to have higher values of fertilization and hatching compared to the group which is not given the addition of curcumin.
KONDISI NEONATUS ANAK BABI YANG DILAHIRKAN OLEH INDUK YANG DISUNTIK GONADOTROPIN SEBELUM PENGAWINAN Friska Mery Montolalu; Anita Esfandiari; Damiana Rita Ekastuti; Wasmen Manalu
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.373 KB) | DOI: 10.19087/jveteriner.2018.19.1.76

Abstract

Injections of sows with pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) prior to mating have been shown to improve endogenous secretions of pregnant hormones that improve and optimize the uterine environment and condition during pregnancy that eventually lead to give birth to superior piglets. This experiment was designed to study the effects of PMSG and hCG injections of sows prior to mating on prenatal growth as indicated by the conditions of the the neonate piglets. Thirty mature female Landrace pigs were divided into 2 groups i.e., 15 pigs were injected with PMSG and hCG prior to mating, and the other 15 pigs were injected with physiological NaCl solution as a control. Before mating, the estrus cycles of the experimental sows were synchronized by injecting prostaglandin two times with 14 days interval. The experimental pigs were injected with PMSG and hCG or 0.90% NaCl solution at the same time with the second prostaglandin injection and were further mated naturally. During pregnancy, the experimental sows were maintained with the normal management condition. The results showed that piglest born to sows injected with PMSG and hCG prior to mating had shorter and faster birth process with higher activities and survivals as compared to those born to control sows. Piglets born by sow that were injected with PMSG and hCG before mating had a more optimum birth weight accompanied by greater body length, limb height, and higher rear limb height. Piglest born to by sows that were injected with PMSG and hCG prior to mating showed superior conditions that would contribute to optimum and superior preweaning and postweaning growths until maturity.
Use of Herb Combination (Jamu Atoke) Before Mating to Improve Rat Pup Health Andriyanto Andriyanto; Leliana Nugrahaning Widi; Hamdika Yendri; Kharisma Mardathilah; Diky Yuliansah; Firda Agustin; Aulia Andi Mustika; Wasmen Manalu
Majalah Kedokteran Bandung Vol 54, No 1 (2022)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v52n4.2629

Abstract

Mortality and health disturbances in children often correlate with maternal health and fertility. Avocado, mung bean sprouts, and holy basil have been traditionally used to improve maternal health, before and during pregnancy. This study was aimed to assess the efficacy of herbal combination of avocado, mung bean sprouts, and holy basil (Jamu ATOKE) in optimizing reproductive health. Eighteen female Sprague Dawley rats (9–10 weeks old, BW: 180–250 g) were randomly divided into 3 treatment groups (n=6 rats for each group) of control, group I and II. ATOKE were added into rats drinking water and consumed for 30 days before pregnancy. After the rats were pregnant and gave birth, pup per parent ratio, pup mortality, and pup health performance (body weight gain, feed and drink consumption, motor activity, pup speed in finding light and feed, red blood cell (RBC) count, white blood cell (WBC) count and differential, SGPT, SGOT, blood urea nitrogen (BUN), creatinine, and proinflammatory mediators (TNF-α and IL-6) were analyzed. Pups born to in group I had a generally better health performance compared to the control group. There was a 100% mortality in the litter of group II due to the excessive aggressive behavior of the dams as a sign of toxicity. Administration of 2.5% Jamu ATOKE showed the best effects on the health and intelligence of the rat pup.
POTENSI EKSTRAK PEGAGAN (Centella Asiatica) DAN KUNYIT (Curcuma longa) UNTUK MENINGKATKAN AKTIVITAS ENZIM GLUTATION PEROKSIDASE (GSH Px) PADA JARINGAN HATI TIKUS Tuti Aswani; Wasmen Manalu; Agik Suprayogi; Min Rahminiwati
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i3.1832

Abstract

This experiment was designed to study the effect of pegagan (Centella asiatica) and turmeric (Curcuma longa) extracts on the activities of glutathione peroxidase (GSH- Px) in liver of white Spraque Dawley rat aged 2 months old with average weight of 200 grams. Pegagan was extracted using infuse method, and turmeric was extracted using dekokta method. The activity of GSH-Px was measured using Flohe and Gunzler’s methods. Extract pegagan and turmeric were assumed to have both preventive and curative treatments. For the preventive treat- ment, extract pegagan and turmeric at concentrations of 22.05 mg/ml : 184.1 mg/ml could increased the activity of GSH-PX enzyme, i.e. 232.60 ± 21.40 mU / mgprotein. This was approximately 79% than the levels of GSH- Px enzyme in normal liver without paracetamol which was 190.78 ± 9.28 mU/mgprotein. For the curative treatment, extract pegagan and tumeric at concentration of 22.05 mg/ml : 184.1 mg/ml could improved GSH- Px enzyme activity (i.e. 239.01 ± 47.40 mU/ mgprotein). This figure was about 92% higher than the levels of GSH-Px enzyme in the liver normal without paracetamol which was 190.78 ± 9.28 mU/mg protein. Pegagan and turmeric extracts with a high concentration could increased the activity of GSH-Px enzyme, thus potential as curative treatment.
Co-Authors - Hernawati . Safrida ., Chairul A Yani ABADI SUTISNA Abdul Zahid Adawiah . Ade Sunarma Ade Sunarma Adi Winanto Adi Winarto Adi Winarto Adi Winarto Agik Suprayogi Agus Oman Sudrajat Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Ahmad Subhan Ahmad Yani ahmad yani Aisjah Girindra Alfred O. M. Dima Alifiana Fitrianingrum Amrozi Andi Mu’nisa Andri Yanto Andriyanto Andriyanto Andriyanto Andriyanto . Andriyanto A Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Anita Esfandiari Anuraga Jayanegara Arief Boediono Aryani Satyaningtijas Aswani, Tuti Aulia Andi Mustik Aulia Andi Mustika Aulia Andri Mustika Awaludin Azam B. Zaidy B P Purwanto B. P. Purwanto Bagus Priyo Purwanto Bambang Gunadi Bambang Gunadi Bambang Gunadi Bambang Kiranadi Bambang Kiranadi BAMBANG KIRANADI Bambang Pontjo Priosoeryanto Benny Heltonika Bondan Achmadi Cece Sumantri Chairul - Chairul Chairul Chairun Nisa Christina Clarice Leksono Cut Dara Dewi Damiana Rita Ekastuti Debby Jacqualine Jochebed Rayer Dedy D. Solihin Deni Noviana Deni Radona, Deni Desrial DEWI APRI ASTUTI Dewi Ratih Agung Priyono Dewi Ratih Agungpriyono Dewi Ratih Agungpriyono Dewi Ratih Agungpriyono Diah Nugrahani Pristihadi Diky Yuliansah Diky Yuliansah Dwi Gunadi Dwi Setyaningsih Dwi Utari Rahmiati Dyah Iswantini Edwin Ligia Sastra Ekowati Handharyani Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Erli Chandra Etih Sudarnika Evy Ayu Arida EVY AYU ARIDA Fachriyan H. Pasaribu Firda Agustin Firda Agustin Firda Agustin, Firda Fitra Aji Pamungkas Fitra Aji Pamungkas Fitra Aji Pamungkas Fitria Senja Murtiningrum Friska Mery Montolalu Ganjar Maulana Nugraha HADI SUNARYO Hamdika Yendri Hamdika Yendri Hamdika Yendri Hamdika Yendri Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamny Sofyan Henni Syawal HERA MAHESHWARI Hera Maheswari, Hera Hernawati Hernawati Hesti Wahyuningsih Huda Shalahudin Darusman Hurip Pratomo Hurip Pratomo Husna, Hanifati I Ketut Mudite I Komang Gede Wiryawan Iman Hernaman Iman Supriatna Ira Agustina Dewi Gandasari Irfan Nurhidayat Irma Herawati Suparto Irzaman, Irzaman Isdoni Bustaman Jakaria Jakaria Jihan Fadilah Rachman Nurullah Jola J.M.R. Londok Joni Haryadi, Joni Kapelle, Imanuel B. D. Kardiyo Praptokardiyo Karin katina Hasya Kasiyati Kasiati Kharisma Mardathilah Kharisma Mardathilah Kiranadi Bambang Koekoeh Santoso Koekoeh Santoso koekoeh santoso Koeswinarning Sigit Komariah Komariah Komariah Krido Brahmo Putro Kusdiantoro Mohamad Kusumorini Nastiti La Eddy La Jumadin Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Widi Leliana Widi Lendrawati Lendrawati Leo Sapelani Soinbala Lies Parede Lina Noviyanti Sutardi Lis Rosmanah Lis Rosmanah Livana Dethris Rawung Lucia Johana Lambey Lynette Ong Huey M. Zairin Junior M.Y Sumaryadi Mas Yedi Sumaryadi Masriani . Meillisa Carlen Mainassy Mien Th. R. Lapian Mohamad Hasil Tamzil Mohamad Yamin Mohamad Yogie Hendrawan Mohammad Miftahurrohman Mokhamad Fakhrul Ulum, Mokhamad Muhaimin Hamzah MUHAMMAD AGUS SUPRAYUDI Muhammad Darjat Darulfalah Muhammad Junior Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Junior Murniaty Simorangkir Nabila Martha Ludi Miftahurahma Nahrowi Nahrowi Nastiti Kusomorini NASTITI KUSUMORINI Nastiti Kusumorini Nastiti Kusumorini NASTITI KUSUMORINI Nelda Fliza Zora Ni Made Ria Isriyanthi Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nurhidayat - Nurhidayat, Nurhidayat Odang Carman Pollung Hasiholan Siagian PONIMAN PONIMAN Pudji Achmadi Purwantiningsih Sugita Putut I. Pudjiono R G Sianturi Raden Roro Sri Pudji Sinarni Dewi Rahminiwati, Min Rarah Ratih Adjie Maheswari Resi Milna Revolson Mege Riasari Gail Sianturi Riasari Gail Sianturi Riasari Gail Sianturi Ridi Arif RIDWAN AFFANDI Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Risna Anggraeni Ronald Tarigan Ronald Tarigan Ronald Tarigan Ronny Rachman Noor Rosdianto, Aziiz Mardanarian Rosdianto, Aziiz Mardanarian Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih Ruqiah Ganda Putri Panjaitan Sabri Mustafa Safrida Safrida SATA YOSHIDA SRIE RAHAYU Sattabongkoch Maneewong Sela Septima Mariya Sharon Aurelia Sharon Aurelia Sharon Aurellia Silmy Kamila Widyanti Silmy Kamila Widyanti Sitti Wajizah Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih Sri Nuryati Sri Subekti Subangkit, Mawar Suhendro, Ikhsan Sumiati Sumiati Sumiati Sumiati Sumiaty Aiba Sunarno . Sunarno s Sunarno Sunarno Sutiastuti Wahyuwardani Sutiastuti Wahyuwardani SYAHRUN HAMDANI NASUTION TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Tiltje Andretha Ransaleleh TOHA SUTARDI Toto Toharmat Tri Budhi Murdiati Tri Budhi Murdiati Tuti Aswani Tutik Wresdiyati Umi Cahyaningsih Uus Saepuloh Wahyuni, Sri Wida Lesmanawati Widanarni Widanarni WIDANARNI WIDANARNI Wiranda G Piliang Wiranda G Piliang Wiranda G. Piliang Wiwin Winarsih Y. Hadiroseyani Yayuk Sri Rahayu Yetmaneli, Yetmaneli Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yulia Yellita Yulvian Sani yuri, Nurasmi yuri Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang Yusfiati Yusfiati Yuvensius Yuvensius Zulfa Zakiah