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Effect of Clove Powder Syzygium aromaticum Supplementation on Growth and Health Status of Cantang Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) in Floating Net Cage Inem Ode; Sukenda Sukenda; Widanarni Widanarni; Dinamella Wahjuningrum; Munti Yuhana; Mia Setiawati
Jurnal Perikanan Universitas Gadjah Mada Vol 25, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.78348

Abstract

Cantang grouper is a hybrid grouper which is one of the main grouper species cultivated in Indonesia. This study aimed to evaluate the growth and health status of cantang grouper in floating net cages supplemented with clove powder. This study used a completely randomized design with three treatments, each with three replications. Two levels of clove powder dose used were 10 and 15 g kg-1 and one treatment without clove powder dose (control). Feed treatment using the repelletting method. Parameters observed were growth, survival rate, levels of malondialdehyde and superoxide dismutase enzyme, blood parameters, phagocity activity, total bacterial count (TBC) and total vibrio count (TVC). The results showed that the treatment of clove powder supplementation in cantang grouper feed for 30 days was able to increase the growth, survival rate, activity of the enzyme superoxide dismutase (SOD), and differential leukocyte (lymphocyte). The application of clove powder in the feed did not significantly affect the total erythrocytes, leukocytes, hematocrit, phagocytic activity, total bacterial count (TBC) and total vibrio count (TVC) of hybrid grouper in floating net cages. The conclusion of this study, administration of clove powder at a dose of 15 g kg-1 feed was able to significantly increased growth, differential leukocyte (lymphocyte), and survival rate of cantang grouper in floating net cages.
APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei)) Widanarni Widanarni; Sukenda Sukenda; Ghita Ryan Septiani
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.787 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5041

Abstract

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.493±0.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.93±0.025-6.97±0.019% and had higher values than controls (A and B) (P0.05). Meanwhile, feed conversion value in C and D (1.54±0.142 and 1.58±0.117) were lower than controls (P0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.
Administration of Microencapsulated Probiotic at Different Doses to Control Streptococcosis in Tilapia (Oreochromis niloticus) DIAH AYU SATYARI UTAMI; WIDANARNI - WIDANARNI; M AGUS SUPRAYUDI
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.398 KB) | DOI: 10.5454/mi.9.1.3

Abstract

This study aimed to produce microencapsulated probiotic and determine its optimal dose to control streptococcosis in tilapia (Oreochromis niloticus). The probiotic used in this study was Bacillus sp. NP5 RfR that was encapsulated by sterile 10% maltodextrin solution and dried by spray dryer. The experimental fish were reared 28 days and fed by the administration of microencapsulated probiotic in feed with different doses (0,5% (A), 1% (B), and 2% (C)) which were 1010 CFU g-1 as the concentration and control without administration of microencapsulated probiotic, including negative (K-) and positive (K+) control. On day 30, all of the fish except K- were challenged by injecting 0.1 mL/fish  of Streptococcus agalactiae (105 CFU ml-1) by intra-peritoneal (IP) route. This study showed that administration of 0.5% microencapsulated probiotic was effective to control streptococcosis in tilapia with higher post-challenge survival rate, better hematological parameter values, and could inhibit S. agalactiae growth in the host target organs.
Immersion Effect of Estradiol-17β on Cannibalism of Asian Redtail Catfish (Hemibagrus nemurus) Post Larvae Benny Heltonika; Agus Oman Sudrajat; Muhammad Junior Zairin; Widanarni Widanarni; Muhammad Agus Suprayudi; Wasmen Manalu; Yani Hadiroseyani
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.38173

Abstract

Highlight Research The dose, duration of immersion, and time interval of immersion of the estradiol-17β affect the rate of cannibalism and increase the seed survival of Asian redtail catfish in low treatment in this research. Over optimum treatment increased the incidence of cannibalism at Asian redtail catfish and decrease growth performance. Treatment of estradiol-17β, give the ability of Asian redtail catfish to live on high density The treatment of estradiol-17β, has an effect on concentration plasma of body testosterone and cortisol   Abstract The cannibalistic behavior of Asian redtail catfish greatly hampers the supply of these fish seeds, invoking the need to be controlled. Estradiol is one of the hormones that has been known to reduce cannibalistic behavior on fish. This study aimed to evaluate the effect of estradiol-17β immersion on the incidence of cannibalism in the rearing of post larvae of Asian redtail catfish. Post larvae was treated with a combination of doses of estradiol hormone 0, 1, and 2 ppm and immersion time of two and four hours with immersion intervals of three and six days with a completely randomized design. Each treatment had a fish density of 10 fish L-1, with mean size individual length of 6.73 ± 0.73 mm (three days of age after hatched), with four replications. Fish were fed with tubifex up until satiation point and reared for 30 days. The results showed that the administration of the estradiol-17β through immersion with a dose of 1 ppm for two hours and interval of six days was able to reduce the level of cannibalism and increase the survivability. A further increase in dose, immersion time, and interval had the opposite effect. Estradiol-17β immersion with certain dose, duration, and interval affected the rate of cannibalism, growth rate, and survival. Estradiol-17β immersion at a dose of 1 ppm for two hours and six days interval was the best to reduce the level of cannibalism and normal mortality in post larvae of Asian redtail catfish.
GROWTH, IMMUNITY, AND RESISTANCE OF CATFISH (Clarias sp.) REARED IN BIOFLOC SYSTEM SUPPLEMENTED WITH Bacillus NP5 AGAINST Aeromonas hydrophila INFECTION Suleman, Gabriella Augustine; Widanarni, Widanarni; Yuhana, Munti; Afiff, Usamah
Indonesian Aquaculture Journal Vol 19, No 1 (2024): (June, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.1.2024.45-56

Abstract

Catfish Clarias sp. is one of the leading commodities in demand and has economic value. Low survival due to cannibalism and disease is a serious problem. To overcome these problems is application of bioflocs and probiotics Bacillus NP5. This study aimed to analyze the appropriate dose of Bacillus NP5 probiotic addition to the biofloc system to improve the health status, growth performance, water quality, and resistance to A. hydrophila. The catfish fry (Clarias sp.) with an average weight of 1.79±0.05 g was used in the experiment for 40 days, reared in tanks with the dimension of 60×30×35 cm3. The experiment applied a completely randomized design (CRD) of five treatments with three replicates, namely KN (negative control), KP (positive control), BFT (biofloc application without probiotic bacteria), BFT4 (biofloc application with Bacillus NP5 104 CFU mL-1), BFT6 (biofloc application with Bacillus NP5 106 CFU mL-1). Treatments tested were challenged with A. hydrophila density 104 CFU mL-1 by immersion, except the negative control. After 40 days of rearing, biofloc and Bacillus NP5 treatments had a significant effect (P < 0.05) on growth performance, immune response, water quality, total bacteria in water and the gut compared to the control treatment (P < 0.05). In addition, total A. hydrophila in liver, kidney and water were lower (P < 0.05) in BFT4 and BFT6 treatments than the control. The conclusion of this study is that the bioflocs supplemented with Bacillus NP5 improved the growth performance, immune response and resistance of catfish to A. hydrophila infection.
COMMERCIAL HERBAL ADMINISTRATION FOR PREVENTING Vibrio parahaemolyticus INFECTION IN VANNAMEI SHRIMP (Litopenaeus vannamei) Putra, The Best Akbar Esa; Widanarni, Widanarni; Wahjuningrum, Dinamella; Yuhana, Munti
Indonesian Aquaculture Journal Vol 19, No 1 (2024): (June, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.1.2024.11-23

Abstract

Vibrio parahaemolyticus is one of the pathogens in crustaceans that can cause mass death in vannamei shrimp farming. This study aimed to evaluate the effect of administering the commercial herbal supplement Phycurma Aquatic (PA) through feeding at different doses to prevent V. parahaemolyticus infection in vannamei shrimp. This study used a completely randomized design (CRD) consisting of five treatments and three replications, which are negative control group, positive control group, and PA at doses of 2.5, 5.0, and 7.5 mL kg-1 feed. Shrimps were reared for 30 days and fed five times a day. On the 31th day, shrimps were challenged with V. parahaemolyticus at a dose of 105 CFU mL-1, except for the negative control group. The results showed that the administration of 5.0 mL kg-1 of PA in the feed gave the best growth performance (P<0.05) compared to other treatments. The administration of 5.0 mL kg-1 PA in feed also enhanced shrimp health status and significantly increased the total hemocyte count, phagocytic activity, respiratory burst, and phenoloxidase. Furthermore, the administration of PA also increased antioxidant activity, reduced malondialdehyde levels, decreased V. parahaemolyticus population in the intestine, and reduced hepatopancreas tissue damage. Moreover, the survival rate of vannamei shrimp before and after the challenge test in the treatment group with a dose of 5.0 mL kg-1 of PA was significantly higher (P<0.05) compared to other treatments.
CANNIBALISM PERFORMANCE OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) FED RATION SUPPLEMENTED WITH DIFFERENT DOSES OF 17α-METHYLTESTOSTERONE Heltonika, Benny; Sudrajat, Agus Oman; Junior, Muhammad Zairin; Widanarni, Widanarni; Suprayudi, Muhammad Agus; Manalu, Wasmen; Hadiroseyani, Yani
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.27-35

Abstract

One of the obstacles in Asian redtail catfish hatchery is the high cannibalism incidence. Cannibalism is associated with aggressive behavior caused by hormonal metabolism especially of testosterone. The purpose of this study was to evaluate the effect of testosterone administration on the cannibalism incidence in the Asian redtail catfish juveniles.  The experiment was conducted by rearing fish with initial length of 4.09±0.19 cm in 16 of 20 L aquariums with a density of 2 fish L-1. The experiment was designed with a completely randomized design with 4 treatments which were different level of 17α-methyltestosterone supplementation in feed, i.e., 0 mg kg-1 feed (A) as control, 7.5 mg kg-1 feed (B), 15 mg kg-1 feed (C), and 30 mg kg-1 feed (D). Each treatment has 4 replications. Fish fed experimental diet (40% protein) 4 times a day to satiation for 30 days. The parameters observed were type and index of cannibalism, aggressive behavior, survival rate, normal mortality, growth performance, hormones concentrations (estradiol, testosterone, and cortisol), and water quality. The results showed that cannibalism type II (the fish eaten completely or missing) and cannibalism index increased with the increasing doses of testosterone administration in the feed with the highest cannibalism incidence was 40.63%. The highest survival rate was found in treatment B (73.75±2.50%) and was not significantly different from the control treatment (69.38±2.39%). No differences in testosterone concentration and in the growth performance among the treatments.  However, there was a trend of decrease in the estradiol concentration of Asian redtail catfish juveniles fed ration supplemented with the increasing doses of 17α-methyltestosterone. Based on the results obtained in this research, estradiol changed in the body's plasma, it appears that there was a role for plasma estradiol concentration in controlling cannibalism of Asian redtail catfish juveniles. It concluded that the testosterone administration affected the cannibalism incidence in the Asian redtail Catfish. 
Bekerja Sama Untuk Menjaga Kebersihan Pantai Wonogoro: Aksi Nyata Untuk Konservasi Bersama LPPM STIE Malangkucecwara dan Mitra Internasional Hariadi, Sugeng; Rahmawati, Rina; Maria, Evi; Pudjiastuti, Widanarni; Aryani, Dwi Nita
J-ADIMAS (Jurnal Pengabdian Kepada Masyarakat) Vol 12, No 1 (2024)
Publisher : (STKIP) PGRI Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29100/j-adimas.v12i1.5533

Abstract

Kebersihan lingkungan pantai merupakan salah satu faktor penting dalam menjaga kelestarian alam dan keberlangsungan pariwisata. Pantai Wonogoro, meskipun memiliki potensi wisata yang besar, masih menghadapi permasalahan terkait sampah organik dan anorganik yang mencemari lingkungan pantai. Untuk mengatasi permasalahan tersebut, Tim Pengabdian Masyarakat STIE Malangkucecwara bekerja sama dengan mahasiswa asing dari International Community Service (ICS) melakukan aksi bersih sampah di Pantai Wonogoro. Kegiatan ini bertujuan untuk meningkatkan kebersihan pantai, menanamkan kesadaran masyarakat tentang pentingnya menjaga kebersihan lingkungan, serta menjadi contoh aksi nyata dalam pelestarian lingkungan. Melalui kegiatan ini, Pantai Wonogoro menjadi lebih bersih dari sampah organik dan non-organik, masyarakat mendapatkan edukasi tentang pentingnya menjaga kebersihan lingkungan, dan tim pengabdian menyumbangkan 6 tong sampah untuk memudahkan warga dalam membuang sampah. Kegiatan ini diharapkan dapat mendorong masyarakat untuk turut berpartisipasi dalam menjaga kebersihan area wisata Pantai Wonogoro
PENINGKATAN PRODUKTIVITAS TAMBAK UDANG VANAME MELALUI PENGEMBANGAN SUMBERDAYA MANUSIA DI DESA KARANGSEWU, KECAMATAN GALUR, KABUPATEN KULON PROGO, DIY : Increasing the Productivity of White Shrimp Ponds Through Human Resources Development in Karangsewu Village, Galur District, Kulon Progo Regency, DIY Diatin, Iis; Budiardi, Tatag; Hadiroseyani, Yani; Effendi, Irzal; Widanarni, Widanarni; Vinasyam, Apriana; Astari, Belinda; Apriani, Ita
Jurnal Pepadu Vol 5 No 4 (2024): Jurnal PEPADU
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v5i4.5895

Abstract

Quality of human resources is a key factor in the success of cultivation. Farmers who have good technical knowledge and skills tend to be more successful in managing their ponds. Therefore, through training and mentoring programs, it is hoped that the knowledge and skills needed to increase shrimp pond productivity can be provided. The purpose of this activity is to improve farmers' understanding of effective and efficient white shrimp cultivation techniques, identify problems, and create networks between farmers to share experiences and information. The training was held in July 2024 in Karangsewu Village, Galur District, Kulon Progo Regency, D.I.Yogyakarta Province. The training attended by 35 participants consisting of white shrimp farmers, fisheries extension workers, and the surrounding community. After completing the training, participants received support in the form of mentoring for shrimp cultivation activities for three months. Pre-tests and post-tests were conducted to measure the increase in participants' knowledge. The results showed that the level of knowledge of participants increased by 6.64%. As many as 79% of participants strongly agreed that the training could improve their knowledge, 71% strongly agreed that the training they attended was relevant to their jobs, and 86% of participants stated that they could develop their potential after attending the program. The results of post-training monitoring showed that productivity of white shrimp pond increased from an average of 12 tons/ha to 23.16 tons/ha. This program not only increases productivity and business sustainability, but also has a positive impact on the economy of the surrounding community. There needs to be a follow-up program to improve shrimp farmers' understanding of financial management, marketing strategies, and the development of value-added products.
Histopathology of liver, kidney, intestine, spleen, and bile of catfish with Jaundice Asrido, Farhan; Nuryati, Sri; Widanarni, Widanarni
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.250-261

Abstract

Jaundice is a disease that can affect catfish, resulting in low or unsalable selling prices. The yellow colour in catfish is associated with tissue/organ disorders, particularly bile or liver function. This study aims to compare the histopathological structure of the liver, kidney, intestine, spleen, and bile of naturally jaundiced catfish and those injected with bacteria associated with jaundice. The study employed the observation method, using three variables: healthy catfish as a control (K), yellow catfish treated with Aeromonas spp. bacteria associated with jaundice (KP), and yellow catfish from the farm (KL). The results of the observation showed that the field scale variable (KL) had a higher level of necrosis damage in each organ compared to the laboratory scale variable (KP). The yellow colour in catfish is caused by disrupted organ tissue, particularly in the bile or liver. This can be observed in the liver, intestines, kidneys, spleen, and bile, where the field variable consistently indicates high levels of necrosis damage, with scores ranging from 1.11 to 2.48. Some of these scores indicate moderate damage, with a necrosis percentage of 40% ≤ P < 60% in the liver, kidneys, and bile organs. Keyword: Clarias sp., histopathology, jaundice, necrosis ABSTRAK Salah satu penyakit yang menyerang ikan lele adalah penyakit kuning atau dikenal juga dengan jaundice, yang dapat mengakibatkan harga jual menjadi rendah atau bahkan tidak dapat dijual. Warna kuning pada ikan lele dikaitkan dengan gangguan jaringan / organ, terutama empedu atau fungsi hati. Penelitian ini bertujuan untuk membandingkan struktur histopatologi organ hati, ginjal, usus, limpa, dan empedu ikan lele yang sakit kuning baik yang alami maupun yang diinjeksi dengan bakteri yang berasosiasi dengan penyakit kuning. Penelitian ini menggunakan metode observasi dengan tiga variabel yaitu ikan lele sehat sebagai kontrol (K), ikan lele kuning yang diberi perlakuan injeksi dengan bakteri yang berasosiasi dengan penyakit kuning yaiatu bakteri Aeromonas spp. (KP), dan ikan lele kuning yang berasal dari farm (KL). Hasil pengamatan yang diperoleh menunjukkan bahwa variabel skala lapangan (KL) memiliki tingkat kerusakan nekrosis paling tinggi pada setiap organnya dibandingkan dengan variabel skala laboratorium (KP). Warna kuning pada ikan lele berhubungan dengan terganggunya jaringan organ terutama empedu atau fungsi hati. Terlihat pada organ hati, usus, ginjal, limpa, dan empedu bahwa variabel lapangan selalu mendapatkan skor tingkat kerusakan nekrosis yang tinggi dengan kisaran nilai skoring 1,11-2,48 yang sebagian tergolong kerusakan sedang dengan persentase nekrosis 40% ≤ P<60% pada organ hati, ginjal, dan empedu. Kata kunci: Clarias sp., histopatologi, nekrosis, penyakit kuning
Co-Authors . Sunarto A. Sunarma A. Suwanto A.J. Sihombing Achmad Farouq Ade Dwi Sasanti ADNI OKTAVIANA Afiff , Usamah Agus Oman Sudrajat Aldy Mulyadin Alfabetian Harjuno Condro Haditomo Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimudin Alimudin, Alimudin Alit Brilliant Angela Mariana Kusumastuti, Angela Mariana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Al Rosjidi Anis Zubaidah Anja Meryandini Anja Meryandini Anjarweni, Vika Atinia Annisa Astri Anggraeni Antonius Suwanto ANTONIUS SUWANTO Ardana Kurniaji Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Asri Sutanti, Asri Asrido, Farhan Astari, Belinda Ayi Santika Bagus Ansani Takwin Bako, Surandha Benny Heltonika Bunyamin Bunyamin Cahyawati, Nadia Catur A. Pebrianto D. Augustine D. Djokosetiyanto D. Meha D. Shafruddin D.D. Mailana D.T. Soelistyowati Daniel Djokosetiyanto DEBY YUNIASARI DEDI JUSADI Dendi Hidayatullah, Dendi Dewi Nurhayati Dewi Rahmi DIAH AYU SATYARI UTAMI DIANA ELIZABETH WATURANGI Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Agung Saputra Dwi Nita Aryani E. Ayuzar Eddy Supriyono Edi Sudiarto Eko Sudjawoto Elizabeth Waturangi, Diana Enang Harris Enang Harris Enzeline, Valensia Evi Maria Fadilah, Iin Nur Fahmi Rajab Fauzi, Arini Resti Ferymon Mahulette Ferymon Mahulette, Ferymon Fiska Puspita Fitria Novianti Frid Agustinus Gentiga Muhammad Zairin Ghita Ryan Septiani Gustilatov, Muhamad H.J. Bugri Hamida Pattah Hamsah Hamsah Hamtini - Hamtini Hany Handajani Hariadi, Sugeng Harton Arfah Hasan Nasrullah Huda Salahudin Darusman Huda Shalahudin Darusman I. Effendi I. Tepu Iis Diatin Iis Sumartini Iman Rusmana Inem Ode Iqbal Kurniawinata, Mohamad Irma Melati Irzal Effendi Istiqomah, Amalia Ita Apriani Jeanni Indah Noermala Julie Ekasari Julyantoro, Pande Gde Sasmita Kautsar, Badar Kukuh Nirmala Lastriliah, Mira Lilik Setiyaningsih Linuwih Aluh Prastiti M. Tri Djoko Sunarno M. Yusuf Arifin M. Zairin Junior M.A. Lidaenni M.S. Arifin Marli, Marli Mas Tri Djoko Sunarno, Mas Tri Djoko Maulana, Fajar Mia Setiawati Mohammad Faizal Ulkhaq MUHAMMAD AGUS SUPRAYUDI Muhammad Junior Zairin Muhammad Subhan Hamka Muhammad Zairin Jr. Mulyasari Mulyasari Mulyasari Mulyasari MUNTI YUHANA Nasrullah, Hasan NISA RACHMANIA MUBARIK Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah O. Carman Ode, Inem Prassetyo Dwi Dhany Wijaya Puguh Widagdo Putra, The Best Akbar Esa Rahmi, Kurnia Anggraini Rakhmawati, Rakhmawati, Ramadhani, Dian Eka Ratnadilla, Thamara Reza Samsudin Rifqah Pratiwi Rina Rahmawati, Rina Rio Yusufi Subhan Rizki Praseto, Rizki Rizkiyanti, Ita Rr. Bellya Anasti Maharani Ruku Ratu Borut S. Sarah Safira Qisthina Ayuningtyas, Safira Qisthina Salamah Salamah Samsu Adi Rahman Saputra, Damar Auliawan Saputri, Rika Ani Septiani, Ghita Ryan Siregar, Khoirotun Nisa SITI MARYAM Siti Munfaqiroh Sri Nuryati Sri Nuryati Suci antoro Sudrajat, R Herman Sugiyo Hadi Pranoto Sujaka Nugraha Sujono Sujono Sukenda Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Suprianto, Dedi Suryadi Saputra Tambun, Andreas Tatag Budiardi Tatik Mufidah Tatik Mufidah Taufiq Abdullah Titin Kurniasih Tri Heru Prihadi Tri Rezeki, Nanda Tsani Untsa, Agista Turnip, Enita Romasni Vinasyam, Apriana W. Efiyanti Wahyu Afrilasari WAODE MUNAENI Wasmen Manalu Wicaksono, Baref Agung Wida Lesmanawati Wijaya, Sella Septian Windu Sukenda Wira H Saputra Wiyarni Wiyarni Y. Hadiroseyani Yani Aryati Yanti Inneke Nababan Yeni Elisdiana Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yuke Eliyani Yuke Eliyani Yulianti, Susy Anisa Yunarty Yunarty Zafril Imran Azwar Zairin Jr., Muhammad