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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Buletin Peternakan Buletin Veteriner Udayana Jurnal Veteriner Prosiding Seminar Biologi Jurnal Kedokteran Brawijaya Dentika Dental Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kesehatan Masyarakat Journal of Dentistry Indonesia Jurnal Ilmu Ternak Veteriner Indonesian Journal of Tropical and Infectious Disease Majalah Kulit, Karet, dan Plastik The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia Indonesian Journal of Biotechnology Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology JCE (Journal of Childhood Education) JURNAL PENDIDIKAN TAMBUSAI JFIOnline Seling: Journal of PGRA Study Program Islamika: Jurnal Keislaman dan Ilmu Pendidikan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Thufuli: Jurnal Ilmiah Pendidikan Islam Anak Usia Dini Dinasti International Journal of Economics, Finance & Accounting (DIJEFA) Jurnal Kajian Ilmiah Annual Conference on Islamic Early Childhood Education Edu Society: Jurnal Pendidikan, Ilmu Sosial dan Pengabdian Kepada Masyarakat Mudabbir: Journal Research and Education Studies Jurnal Kedokteran Hewan Jurnal Multidisiplin Sahombu Triwikrama: Jurnal Ilmu Sosial VISA: Journal of Vision and Ideas
Widya Asmara
Department Of Microbiology, Faculty Of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
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Analisis Genetik Gen Protective Antigenic pada Bacillus anthracis Isolat Jawa Tengah dan Yogyakarta (GENETIC ANALYSIS ON PROTECTIVE ANTIGENIC GENE OF BACILLUS ANTHRACIS ISOLATES OF CENTRAL JAVA AND YOGYAKARTA) Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to determine sequence and genotype diversity of protective antigenic gene ofBacillus anthracis isolated from Central Java and Yogyakarta. Pag-A gene which encodes for antigenicprotein is one toxin component and the virulent factor of B. anthracis. As many as five isolates fromSemarang, Sragen, and Boyolali (Central Java) and Sleman (Yogyakarta) were used. The gene wassequenced and amplified used three set of primers PA1857/PA2436, PA8/PA5, and PA-5F/PA-5R. Theresult showed that the nucleotide sequences of gene from five isolates were identical and only had onenucleotide difference as compared to B. anthracis sterne M22589. All isolates were confirmed as genotypebased on pag-A sequence. It was concluded that all B. anthracis from Central Java and Yogyakarta haveidentical pag-A sequence and belong to genotypt-1. Further studies are needed to investigate B. anthracisisolates from other regions of Indonesia.
Non Coding Region dan Amino Terminus Gen Polimerase Asidik Virus Avian Influenza Subtipe H5N1 Asal Hewan Indonesia Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The knowledge on the species adaptation factor of avian influenza virus of H5N1 subtype (AIV H5N1)is very important as a signal for the emergence of a new strain with pandemic potential. This research wasconducted to find out the sequence variation of the Non-Coding Region (NCR) and Coding Region (CR) of 5’-terminal cRNA of the polymerase acidic (PA). Total RNA from twenty six (26) avian influenza virussubtype H5N1 isolates were amplified using reverse-transcriptase-polymerase chain reaction (RT-PCR)with a universal forward primer for influenza virus and specifically designed backward primers. Nineteen(19) gene fragments of PA could be amplified. RT-PCR products were sequenced and analyzed using Mega4 software. The length of NCR of PA gene was found to be 24 bases and conserved. A/T composition of PANCR was 58.3%. Species and geographical specificity could not be found in the genetic distance, the aminoacid polymorphism, as well as the phylogenetic analysis of the CR. RNA sequencing is discussed andrecomended to be studied further.
Antigen Ekskretori-Sekretori Cacing Jantung (Dirofilaria immitis) Jantan dan Betina yang Berpotensi Sebagai Marka Diagnosis (EXCRETORY-SECRETORY ANTIGENS OF MALE AND FEMALE HEART WORMS (DIROFILARIA IMMITIS) WHICH POTENTIALLY AS A DIAGNOSTIC MARKER) I Gusti Made Krisna Erawan; Ida Tjahajati; Wisnu Nurcahyo; Widya Asmara
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Heart worm (Dirofilaria immitis) is the causative agent of a serious parasitic disease in dogs.Dirofilariasis is generally diagnosed by microfilariae examination and specific antigen testing. Microfilariaeexamination has low sensitivity due to occult infections. The available antigen test at this time is able todetect circulating antigens secreted by adult female worms only. The aim of the present study was toidentify male (MES) and female (FES) heart worms excretory-secretory antigens which have the potentialas a diagnostic targets. Identification of antigen was done by sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE) and Western Blotting analysis. The results of this study indicated that therewere differences between the MES and the FES profiles. The results showed 12 bands in MES (14–118kDa) and 18 bands in FES (10–205 kDa). Protein with a molecular weight of 59 kDa has the potential asdiagnostic markers of dirofilariasis.
VARIATION OF NON-CODING REGION AND CODING REGION OF 5’-TERMINAL CRNA OF POLYMERASE BASIC 1 OF AVIAN INFLUENZA VIRUS SUBTYPE H5N1 Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The sequence of the Non-Coding Region (NCR) and Coding Region (CR) of 5’-terminal cRNA of thepolymerase basic 1 (PB1) gene as a major factor for the species adaptation of avian influenza virussubtype H5N1 (AIV H5N1) has been analysed. The information could be a virological signal for theemergence of a new strain with pandemic potential. Total RNA from twenty six (26) avian influenzasubtype H5N1 isolates were amplified using reverse-transcriptase-polymerase chain reaction (RT-PCR)with a universal forward primer for influenza virus and specifically designed backward primers. Fifteen(15) PB1 gene fragments could be amplified. RT-PCR products were sequenced and analyzed using Mega4software. The length of NCR of PB1 gene was found to be 24 bases and mostly shows conserved sequence,with an exception of Dk/Badung/2006 isolate which has C-7T substitution. A/T composition of PB1 NCRwas 54,2%, while the Dk/Badung/2006 isolate was 58,3%. Species and geographical specificity could not befound in the genetic distance, the amino acid polymorphism, as well as the phylogenetic analysis of t
Klasifikasi Numerik-fenetik Salmonella typhi Asal Jawa Tengah dan Daerah Istimewa Yogyakarta Berdasarkan Hasil Karakterisasi Fenotipik Sri Darmawati; Langkah Sembiring; Widya Asmara; Wayan T. Artama
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.67

Abstract

Demam tifoid adalah penyakit endemis yang disebabkan oleh strain bakteri S. typhi, bakteri tersebut dapat dikelompokkan berdasarkan perbedaan mikromorfologi, morfologi koloni, penggunaan sumber karbon, produksi enzim, kemampuan mendegradasi makromolekul. Oleh karena itu penelitian ini bertujuan melakukan klasifikasi numerik-fenetik 4 starin S. typhi asal Jawa Tengah dan 2 strain asal DIY dengan 2 strain acuan S. typhi NCTC 786 dan BLKS berdasarkan karakterisasi fenotipik dengan analisis hubungan similaritas yang didasarkan atas analisis Simple Mattching Coefficient (SSM) serta algoritme UPGMA (unweighted pair group methode with averages) yang kemudian dipresentasikan dalam bentuk dendogram. Hasilnya dapat dikelompokkan menjadi empat klaster, klaster pertama beranggotakan 3 strain berasal dari Jawa Tengah dan 1 strain acuan BLK Semarang (similaritas 100%). Klaster kedua beranggotakan satu strain acuan S. typhi NCTC 786 (similaritas 98,6%) dengan klaster pertama. Klaster ketiga beranggotakan 1 strain MDA dari Jawa Tengah (similaritas 96,8%) dengan klaster pertama dan kedua. Klaster keempat beranggotakan 2 strain S. typhi asal DIY yang memiliki (similaritas 96,4%) dengan ketiga klaster lainnya.
Kerentanan Palatogenesis Mencit (Mus musculus L.) terhadap Induksi Cleft Palate TCDD Salomo Hutahaean; Soesanto Mangkoewidjojo; Mammed Sagi; Widya Asmara
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.100

Abstract

Telah dilakukan percobaan untuk menentukan tahapan palatogenesis pada mencit (Mus musculus L.) yang rentan terhadap efek polutan 2,3,7,8-Tetraklorodibenzo-p-dioksin (TCDD). Percobaan dirancang mengikuti Rancangan Acak Lengkap dengan pola faktorial (4X3). Empat puluh delapan ekor mencit bunting dicekok TCDD dengan dosis 0 (kontrol), 5, 10, atau 20 μg/kg bb. Perlakuan diberikan pada hari kebuntingan (Hk) 9−10, 11−12, atau 13−14. Mencit kontrol dicekok pelarut saja (98,5% minyak wijen + 1,5% DMSO). Pada Hk 18 mencit dibius lalu dibunuh dengan teknik cervical dislocation, persentase fetus cleft palate (cp) dihitung, derajat penutupan palatum diberi skor, preparat dengan ketebalan 6 µm dibuat, dan mikrostruktur kraniofasial diamati. Hasil menunjukkan, pemberian TCDD antara hari ke 9 dan 12 menginduksi cacat cp, dengan kecenderungan hasil tertinggi pada pemberian Hk 910. Perlakuan TCDD dosis 10 atau 20 μg/kg bb pada Hk 910 menghasilkan fetus cacat cp >90%. Persentase fetus cp tetap tinggi pada pemberian Hk 1112, khususnya pada kelompok dosis 20 μg/kg bb (87,3%). TCDD dosis terendah (5 μg/kg bb) menginduksi cp dominan bercelah sempit, menunjukkan adanya hambatan pada tahap fusi. Dosis 10 dan 20 μg/kg bb menginduksi cp bercelah sedang atau lebar, mengisyaratkan terjadi hambatan pada tahap inisiasi atau elevasi. Disimpulkan, seluruh tahapan palatogenesis rentan terhadap efek TCDD, namun tahap paling rentan adalah tahap fusi palatum.
Analisis Kuantitatif Sel Purkinje Cerebellum Mencit (Mus musculus L.) setelah Induksi Ochratoksin A Selama Periode Organogenesis Arum Setiawan; Mammed Sagi; Widya Asmara; Istriyati Istriyati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.108

Abstract

Penelitian ini bertujuan mengetahui jumlah sel Purkinje cerebellum anak mencit umur 21 hari (pascasapih) setelah induksi Ochratoksin A selama periode organogenesis. Tiga puluh ekor mencit bunting dibagi secara acak menjadi 5 kelompok perlakuan dengan masing-masing 6 ulangan. Ochratoksin A dilarutkan dalam Sodium Bicarbonat, diberikan secara oral pada saat kebuntingan hari ke 7 sampai hari ke -14. Dosis perlakuan Ochratoksin A adalah 0,5 ; 1,0; 1,5 mg/kg bb dan sebagai kontrol tidak diberi perlakuan, serta kontrol placebo diberi perlakuan pelarut Sodium Bicarbonat. Induk mencit dipelihara sampai melahirkan. Pada umur ke 21 hari (pascasapih), anak mencit dikorbankan dan diambil bagian otaknya. Otak mencit selanjutnya dipreparasi dengan metode parafin dan pewarnaan menggunakan pewarnaan Haematoksilin Eosin. Data jumlah sel Purkinje dianalisis dengan Anava Satu Arah dan dilanjutkan dengan uji DMRT untuk mengetahui beda nyata antar perlakuan. Hasil penelitian menunjukkan bahwa Ochratoksin A yang diberikan pada mencit bunting selama periode organogenesis menyebabkan terhambatnya pertumbuhan jumlah sel Purkinje mencit perlakuan yang ditandai dengan semakin menurunnya jumlah sel Purkinje dibandingkan dengan kontrol dan kontrol placebo.
Histopatologi Ikan Kerapu Macan yang Diimbuhi Bakteri Asam Laktat dan Diuji Tantang Vibrio alginolyticus (HISTOPHATOLOGY OF TIGER GROUPER SUPPLEMENTED WITH LACTIC ACID BACTERIA AND CHALLENGED BY VIBRIO ALGINOLYTICUS) Nursyirwani .; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Triyanto .
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Supplementation of lactic acid bacteria (LAB) as probiotic in aquaculture has been reported toincrease fish growth and enhance their resistance against diseases. The aim of this study was to figureout histological changes of tiger grouper (Epinephelus fuscoguttatus) fed with LAB isolates followed bycha
Karakterisasi protease Bacillus sp. UGM5 Titik Purwati Widowati; Soemitro Djojowidagdo; Setiyono Setiyono; Widya Asmara
Majalah Kulit, Karet, dan Plastik Vol 14, No 26 (1999): Majalah Barang Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1359.555 KB) | DOI: 10.20543/mkkp.v14i26.297

Abstract

The objective of this experiment is to indentify the characters of proetease produced by Bacillus sp.UGM5.the protease secreted by Bacillus sp.UGM5 was first isolated,purified and then charactirezed.The crude enzyme has spesific actifity of 1.14 U/mg,however,the spesific activity of purified enzyme was increased by 23.8 times fold and recovery was 33.69%.The Page of nondenatured crude enzymes showes two type of proreases,however ,the SDS-Page of denatured purified enzyme showed four protein-bends with molecular weights of 55.5 kDa,18kDa respecetively.The optimum pH and temperature for the enzyme acrivity are 8.5 and 420C and belongs to serin protease type,with Km 3 X 10-3mM and Vmax 0.0890mM/30 minutes.The activity is not inhibited by Ca+2,Fe+2 and EDTA. INTISARITujuan penelitian ini adalah untuk mengkarakterisasi  protease yang diproduksi  Bacillus sp. UGM5. Protease yang disekresi oleh Bacillus sp.UGM5 pertama-tama diisolasi,dipurifikasi kemudian dikarakterisasi.Enzim kasar mempunyai spesifik 1,14 U/mg.Aktivitas spesifik enzim yang dimurnikan meningkat 23,8 kali dengan recovery 33,69%.Elektroforesis gel poliakrilamid enzim yang dipurifikasi dan didenaturisasi menunjukan ada empat pita protein dengan berat protein masing-masing 55,5 kDa,51 kDa,18 kDa,dan 15,5 kDa .ph dan temperatur optimum aktivitas enzim adalah 8,5 dan 420C,termasuk tipe protease serin,dengan nilai Km 3 X10-3mM dan Vmax 0,0890mM/30menit.Aktivitas enzim tidak dihambat oleh adanya ion Ca2+,Fe+3 dan EDTA.
PHYLOGENETIC PROFILE OF ESCHERICHIA COLI CAUSING BLOODSTREAM INFECTION AND ITS CLINICAL ASPECT Osman Sianipar; Widya Asmara; Iwan Dwiprahasto; Budi Mulyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 1 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i1.1152

Abstract

Escherchia coli merupakan satu dari bakteri yang paling sering ditemukan dalam infeksi aliran darah. Tujuan penelitian ini adalahuntuk mengeksplorasi profil filogenetik E. coli yang menyebabkan infeksi aliran darah dan aspek klinisnya. Ini merupakan penelitianobservasional yang melibatkan 12 subjek yang menderita infeksi aliran darah yang disebabkan oleh E.coli. Isolat klinis E.coli serta hasiluji kepekaan antimikroba diperoleh dari metode kaldu microdilution otomatis. Data klinis diperoleh dari rekam medis dan analisisfilogenetik yang dilakukan dengan polymerase chain reaction menggunakan gena chuA dan YjaA. Data dianalisis dengan menggunakanstatistik deskriptif. Sumber infeksi ini berasal dari saluran kemih, paru-paru, saluran pencernaan dan kulit yang ditemukan dalam 7kasus. Namun, sumber infeksi tidak diketahui dalam 5 kasus. Sebagian besar subjek adalah pria dewasa dengan keganasan sebagaipenyakit yang mendasarinya. Escherichia coli sebagai etiologi infeksi aliran darah sebagian besar (75%) menghasilkan enzim ESBL danresistensinya terhadap antimikroba seperti ampicilin, ampicilin/sulbactam, ceftazidime, ceftriaxon, cefepime, aztreonam, ciprofloxacindan trimetropim-sulfamethoxazol yang cukup tinggi. Kelompok filogenetik dari isolat klinis ini sebagian besar (75%) adalah grup B2dan grup D yang dikenal sebagai strain virulen ekstraintestinal. Isolat klinis yang tersisa (25%) dapat digolongkan sebagai kelompokfilogenetik A atau B1 dimana kelompok A dikenal sebagai strain komensal.
Co-Authors . Sismindari ., Wdjijono A. Alimuddin Adi Heru Sutomo Aditya Krishar Karim Aditya Krishar Karim AETH. Wahyuni AETH. Wahyuni, AETH. Ag. Yuswanto Agnes Sri Harti Agnes Sri Harti Agnesia Endamg Tri Hastuti Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agus Eko Srihanto Agus Eko Srihanto, Agus Eko Agustinus Joko Nugroho Agustinus Joko Nugroho, Agustinus Joko Aisida, Sufinatin Alma Linggar Jonarta Ambar Pertiwiningrum Andini, Syarifah Anwar Rosyidi April H Wardhana Arfian Rahma Shanti Artama , Wayan T. Arum Setiawan Arum Setiawan Asih Kurniawati Bambang Hariono Bambang Sumiarto Bambang Sutrisno Bancin, Girang Stevani Banun Kusumawardani Boy Bachtiar Budi Mulyaningsih Budi Mulyono BUDI SETIADI DARYONO Budi Setiadi Dayono Charles Rangga Tabbu Charles Rangga Tabbu Chatarina Behar Chatarina Behar, Chatarina Darmawati , Sri Darmayanti, Hervina Puspita Dewi Noor Hidayati Dewi Seswita Zilda Dito Anggoro Djaswadi Dasuki Doddi Yudhabuntara Dwi Aji Nugroho, Dwi Dyah Haryuningtyas Dyah Haryuningtyas Dyah Irnawati Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elfayetti Elfayetti Elsa Kardiana Eni Harmayani Eni Harmayani Enny Yusuf Wachidah Yuniwarti Ety Aryati Ety Aryati, Ety Febriani Putri, Devita Fiska Salsabilla Gaol, Riski Fanni Lumban Gintung Patantis Gintung Patantis Gusti Ayu Yuniati Kencana Hafiza, Rohmania Zuhro Hamzah Muhammad Mardi Putra hanifah hanifah Hanny Hafiar Hardyanto Soebono Hardyanto Subono Harefa, Meilinda Suriani Harefa, Sri Aswinda Hari Eko Irianto Hasibuan, Novita Hasibuan, Novita Annisah Heru Susetya Hutauruk, Mutiara Cristeofani I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Wayan Suardana I. Istriyati Ida Tjahajati Iisnawati, Iisnawati Ika Dyah Kusumawati Ika Dyah Kusumawati, Ika Dyah Indwiani Astuti Istriyati ., Istriyati Istriyati Istriyati Istriyati Istriyati Istriyati, . Istriyati, . Iwan Dwiprahasto JAKA WIDADA Juni Handajani Kardians, Elsa Karna Wijaya Kerin Sisca Octaviani Luahambowo Khairunnisa Khairunnisa Khairunnisa Nasution Khrisdiana Putri Khrisdiana Putri, Khrisdiana Koichi Akiyama Krisdiana Putri Kumalasari, Eliana Dwi Langkah Sembiring LANGKAH SEMBIRING Langkah Sembiring Lina Sri, Widyawati Liza Angeliya M. Haryadi Wibowo M. Mustofa Mammed Sagi Mammed Sagi Mangkoewidjojo , Soesanto Marla Anggita Marpaung, Viviana Marsetyawan Soesatyo Marsetyawan Soesatyo Masashi Kawaichi Masashi Kawaichi, Masashi Maxs Urias Ebenheizer Sanam Meilinda Suriani Harefa Michael Haryadi Wibowo MM.Firdiana Krisnaningsih Mustofa M, Mustofa Mustofa Mustofa Niken Irfa Nastiti Ning Rintiswati Ning Rintiswati Nobuyuki Harada Nursyirwani, Nursyirwani Nurul Islamiyah Nuryono ., Nuryono Nyoman Reishita Andriyani Osman Sianipar Prasetyo, Dimas Bayu Purba, Tondang Raja Pangihutan Purnama Edy Santosa Purwoko, Agus Putra, Mulhady Raden Wisnu Nurcahyo Rahma, Mawaddah Rahmat Setya Adji Rahmat Setya Adji Regina TC Tandelilin Reni Nurjasmi Reni Nurjasmi, Reni Rini Widayanti Risbue Siregar Rosa Delima Lumbantungkup S Muharsini S Rahmah Umniyati, S Rahmah S. Sismindari Sabela, Serli Sagi , Mammed Salomo Hutahaean Salsabilla, Friska Santoso, Ferdinand Prayogo Cahyo Sarwo Edy Wibowo SATRIYAS ILYAS Sebastian Margino Sebastian Margino Sembiring , Langkah Sembiring, Samariana Br Setiawan , Arum Setiyono Setiyono Setiyono Setiyono Setyawan Budiharta Sidna Artanto Sidna Artanto Sidna Artanto, Sidna Simaremare, Ermas Simarmata, Claudia Grace Siregar, Risbue Sismindari . Siti Sunarintyas Sitti, Ummiyati Rahmah Soemitro Djojowidagdo Soesanto Mangkoewidjojo Sri Darmawati Sri Darmawati Sri Darmawati Sri Lestari Subronto Prodjoharjono Sugeng, Mardihusodo Juwono Sugiharto Sulastri, Yati Surya Amanu Surya Amanu Surya Amanu Surya Amanu Susi Iravati Susi Iravati Susi Maulida, Susi Syaiful Anwar Syari’I Damanik, Muhammad Ridha Syukri Hidayat Titik Purwati Widowati Titik Purwati Widowati, Titik Purwati Tiyas Tono Taufiq Tondang Raja Pangihutan Purba Tri Ratnaningsih Tri Untari Tri Untari Tri Untari Tri Wibawa Triyanto . Tsutomu Nohno Vivina Marpaung W. Widjijono W. Widodo Wajar, Dony wayan T Artama wayan T Artama Wayan T. Artama Wayan T. Artama Wayan T. Artama Wayan Tunas Artama Wayan Tunas Artama Widagdo Sri Nugroho Widagdo Sri Nugroho Widodo Widodo Widya Hary Cahyati Wisnu Nurcahyo Wulandari, Anna Yahya, Adibah Yatri Drastini Yulita Kristanti Yundari, Yundari Yuni Wijayanti Yusro Nuri Fawzya Yusro Nuri Fawzya Zahwa, Afitzka Al Zilda, Dewi Zeswita