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DREB1 Gene in Regulating Drought Tolerance in Cereal Plants Todingan, Ajeng Klarica; Aprilian Pailang, Agista; Hala, Yusminah
Bioeksperimen: Jurnal Penelitian Biologi March 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10172

Abstract

This study aims to explain the role of the DREB1 gene in increasing drought tolerance in plants with an emphasis on recent advances in genetic engineering. In this research, the method used is a literature review by collecting library data, reading and taking notes, and critically managing research data related to the DREB1 gene. In this research, data analysis starts by examining the results from studies that are most relevant to the research problem. The results of the literature review obtained indicate that the DREB1 gene is a transcription factor that plays a role in regulating a number of other genes related to drought characteristics. The DREB genes participate in hormone signalling pathways and regulate proteins that interact with downstream genes to improve plant stress tolerance. When there is a drought, seedlings that have the DREB gene inserted have a survival rate of 36%, while plants without the DREB gene all die. These results indicate that the DREB1 gene is a positive regulatory gene for heat tolerance in plants. In this case, the transgenic lines keep their stomata partially closed, which helps maintain a minimum transpiration rate to cool the canopy and reduce the temperature of both the canopy and leaves. We achieve this by coordinating the control of stomata, minimising water loss, and preserving cell turgor, all of which prevent the plant from dying.
Enhancing Student Motivation and Learning Outcomes Through Teams Games Tournament Integration with Wordwall Wahidatul Munandar; Yusminah Hala; Syamsiah Syamsiah
Journal of Innovation and Research in Primary Education Vol. 5 No. 1 (2026)
Publisher : Papanda Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56916/jirpe.v5i1.2644

Abstract

This quasi-experimental study investigated the differential effects of Teams Games Tournament (TGT) learning model with and without Wordwall integration on eleventh-grade biology students' motivation and learning outcomes at SMAN 8 Gowa, Indonesia. Seventy-two students were assigned to two experimental groups: R1 (TGT with Wordwall, n=36) and R2 (TGT only, n=36). Data were collected using a 20-item motivation questionnaire and a 30-item achievement test administered as pretests and posttests. Statistical analyses included descriptive statistics, one-way ANOVA, and normalized gain (N-Gain) calculations. Results revealed significant differences favoring TGT-Wordwall integration for both motivation (F(1,70)=38.42, p<.001, η²=.354) and learning outcomes (F(1,70)=86.77, p<.001, η²=.554). The R1 group achieved high N-Gain scores (g=0.78 for motivation; g=0.82 for learning outcomes) compared to R2's moderate gains (g=0.50 and g=0.63 respectively). Categorical analysis showed 66.7% of R1 students reached adequate or good performance levels versus only 8.3% in R2. These findings demonstrate that integrating interactive digital platforms with cooperative learning models substantially amplifies instructional effectiveness. The study provides empirical evidence supporting technology-enhanced pedagogy in secondary education and offers practical implications for educators implementing 21st-century learning approaches in biology instruction.
Predicting Biology Identity from Plant Literacy, Systems Thinking, and Reflective Judgment: A PLS-SEM Approach Ismail Ismail; Yusminah Hala; Nurhayati B; Suhartini Azis; Asham Bin Jamaluddin
International Journal of Educational Practice and Policy Vol. 4 No. 1 (2026): March-April 2026
Publisher : PT. Global Research Collaboration

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.66314/ijepp.v4i1.418

Abstract

This study examined the relationships among plant literacy, systems thinking, reflective judgment, and biology identity among university students. Using a quantitative non-experimental approach with a cross-sectional survey design, data were collected in January 2026 from students of the Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar. The data were obtained through a structured questionnaire using a 1–7 Likert scale and analyzed with Partial Least Squares Structural Equation Modeling (PLS-SEM). The results showed that the measurement model met the required reliability and validity criteria, with all indicator loadings above 0.70, AVE values above 0.50, and HTMT values below 0.90. In the structural model, plant literacy had a positive and significant effect on biology identity (β = 0.603, p < 0.001), and reflective judgment also had a positive and significant effect (β = 0.275, p < 0.001). In contrast, systems thinking had a negative but non-significant effect (β = -0.026, p = 0.647). The model explained 68.6% of the variance in biology identity. These findings indicate that biology identity is more strongly associated with students’ connection to core biological content and reflective engagement with knowledge than with systems thinking as a direct predictor.
EKSTRAK TANAMAN KUNYIT (Curcuma longa L.) SEBAGAI AGEN BIOPROTEKTAN TANAMAN PANGAN: LITERATUR REVIEW Rijal, Syamsu; Hala, Yusminah
JURNAL BIOSENSE Vol 9 No 2 (2026): Edisi April 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i2.6623

Abstract

Ketergantungan terhadap pestisida sintetis dalam sistem budidaya tanaman pangan menimbulkan berbagai dampak negatif, seperti residu pada pangan, pencemaran lingkungan, dan resistensi hama serta patogen. Berbagai tanaman obat dan rempah telah dieksplorasi sebagai sumber senyawa bioaktif untuk dikembangkan menjadi bioprotektan. Penelitian ini menggunakan metode literatur review. Salah satu kandidat penting adalah kunyit (Curcuma longa L.), yang kaya senyawa kurkuminoid dan minyak atsiri. Literatur menunjukkan bahwa ekstrak kunyit memiliki aktivitas antijamur, antioomiset, insektisida, dan nematisida terhadap beragam organisme pengganggu tanaman, baik pada fase budidaya maupun pascapanen. Review ini merangkum perkembangan penelitian mengenai fitokimia, mekanisme aksi, spektrum target organisme, serta prospek formulasi dan aplikasi ekstrak kunyit sebagai agen bioprotektan tanaman pangan yang ramah lingkungan.
INOVASI BIOREMEDIASI DALAM MENGATASI PENCEMARAN LINGKUNGAN: SYSTEMATIC LITERATURE REVIEW Kadir, St. Fatima; Ahqaf, Nurul Afifah; Hala, Yusminah
SCIENCE : Jurnal Inovasi Pendidikan Matematika dan IPA Vol. 6 No. 2 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia (P4I)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/science.v6i2.9939

Abstract

ABSTRAK Peningkatan pencemaran lingkungan global oleh hidrokarbon, logam berat, dan polutan baru menuntut solusi pemulihan yang berkelanjutan mengingat keterbatasan metode fisik dan kimia konvensional. Bioremediasi hadir sebagai alternatif ramah lingkungan, tetapi temuan inovasi terbarunya masih terfragmentasi dalam berbagai literatur tanpa adanya kajian komprehensif yang mengevaluasi efektivitas gabungan teknologi tersebut. Penelitian ini merupakan Systematic Literature Review (SLR) yang bertujuan untuk memetakan perkembangan inovasi bioremediasi terkini dan mengevaluasi efektivitas gabungan (hibridisasi) teknologinya. Metode penelitian mengadopsi protokol PRISMA dengan menelusuri pangkalan data Scopus (rentang tahun 2020–2026) yang secara sistematis menyaring dan menghasilkan 18 artikel jurnal relevan untuk dianalisis secara tematik. Hasil sintesis literatur menunjukkan adanya pergeseran paradigma bioremediasi dari pendekatan konvensional yang cenderung mengandalkan agen biologis tunggal dan proses yang terpisah, menuju pendekatan terintegrasi berbasis sistem hibrida yang mengombinasikan mikroba indigen, strategi peningkatan bioavailabilitas, serta teknologi rekayasa modern. Pergeseran ini tercermin dalam empat temuan utama, yaitu: (1) eksplorasi galur mikrobioma lokal (indigen), (2) pemanfaatan agen pemicu ketersediaan polutan (bioavailabilitas) seperti biosurfaktan dan quorum-sensing, (3) hibridisasi sistem biologis dengan rekayasa modern (seperti modifikasi genom CRISPR-Cas9 dan Sistem Bioelektrokimia Mikroba), serta (4) identifikasi tantangan implementasi dan kesenjangan adopsi teknologi di tingkat lokal. Kesimpulan penelitian ini menegaskan bahwa integrasi keempat inovasi mutakhir tersebut menjadikan bioremediasi sebagai salah satu intervensi yang adaptif, efisien, dan berkelanjutan dalam pemulihan ekosistem, sehingga mampu mencegah kerusakan ekologi jangka panjang. Pendekatan ini berimplikasi pada peningkatan efisiensi degradasi polutan, pengurangan risiko polusi sekunder, serta percepatan pemulihan kualitas lingkungan.
ADHERENCE AND PATHOGENICITY ASSAY OF VIBRIO HARVEYI IN TIGER SHRIMP (PENAEUS MONODON) LARVAE FOR SCREENING BIOCONTROL AGENT SUWANTO, ANTONIUS; HALA, YUSMINAH; AFFANDI, RIDWAN; Jr., MUHAMMAD ZAIRIN
BIOTROPIA No. 18 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2002.0.18.171

Abstract

Rifampicin-resistant marker was employed as a reporter to detect the adherence and colonization of V. harveyi  in shrimp larvae. Vibrio harveyi P1B  and YA32.2 were isolated from dead shrimp larvae in Besuki, Northern Coast of East Java, while V. harveyi HB3, was isolated from pristine sea water in Pacitan, Southern Coast of East Java. Vibrio metschnikovii used as biocontrol agent was isolated from healthy shrimp larvae in Serang, West Java. Spontaneous mutation was conducted to generate V. harveyi P1B, YA32.2 and HB3 resistant to rifampicin. These mutants exhibited similar survival ability to their parental (wild type) strains. Significant larval mortality was observed in shrimp larvae inoculated with YA32.2 than that of larvae inoculated with P1B. Larvae  inoculated with HB3 showed the lowest mortality. Bacterial cell count of Vibrio Rf*  in dead  larvae were 103-104 cells/larvae. Isolates of Vibrio metschnikovii Z and M as biocontrol candidates effectively reduced the growth and adherence ability of YA32.2 to shrimp larvae. Larval mortality in rearing water inoculated simultaneously with YA32.2 and V. metschnikovii was lower than the one inoculated with YA32.2 alone. Therefore, Vibrio metschnikovii Z or M could be developed as an effective probiotic or biocontrol agent for V. harveyi in shrimp hatcheries. Key words :   Biological control/Vibrio metschnikovii/shrimp \arvae/Penaeus mwu«fon/pathogenicity assay/Vibrio harveyi
The Effect of Android-Based Digital Poster Learning Media on Students' Digital Literacy in Ecosystem Suriyanto; Yusminah Hala; A. Mushawwir Taiyeb
Jurnal Penelitian Pendidikan IPA Vol 11 No 6 (2025): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i6.11495

Abstract

This research is motivated by the low digital literacy of biology of class X students of SMA Negeri 2 Luwu Timur. This study aims to determine the effect of android-based digital poster learning media on students' digital literacy in ecosystem material. This research is a quasi-experimental study with a Nonequivalent Control Group Design research design. The population of this study was all class X with a sample of 2 classes selected randomly. Which consists of 1 experimental class taught using android-based digital poster media, and 1 control class taught using wordwall-based open the box media. Data collection using a questionnaire given before and after treatment in the experimental class and control class. The results of the hypothesis testing at the digital literacy level showed a significance value of 0.023. This shows a sig value <α (0.05) so it can be concluded that H0 is rejected and H1 is accepted. This means that there is a difference in the influence of digital literacy of students taught using Android-based Digital Poster learning media and wordwall-based Open the Box learning media in class X Ecosystem material.
Peer Tutoring for Sustainable Biology Education: Enhancing Student Activity, Motivation, and Learning Outcomes in the Respiratory System Unit Nurul Arini; Yusminah Hala; Rachmawaty; Firdaus Daud; Ismail
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.14096

Abstract

This study aims to analyze the effect of the peer tutoring method on learning activity, learning motivation, and biology learning outcomes of grade XI students at SMAN 15 Gowa. This quasi-experimental research employed a pretest-posttest control group design involving 54 students divided into experimental (n = 27) and control groups (n = 27). Learning activity was measured using observation sheets based on Paul B. Diedrich indicators, motivation was assessed through questionnaires using Keller's ARCS model, and learning outcomes were evaluated using cognitive tests consisting of 25 multiple-choice and 5 essay questions based on revised Bloom's taxonomy. Data were analyzed using Independent Samples t-test to compare groups and Paired Samples t-test to analyze improvements within groups, with a significance level of α = 0.05. The results showed significant effects of peer tutoring on learning activity (p = 0.015), motivation (p = 0.000), and learning outcomes (p = 0.000). The experimental group demonstrated higher mean scores in activity (25.93 vs 24.44) and substantial improvements in motivation (N-Gain 0.61, medium category vs 0.12, low category) and learning outcomes (N-Gain 0.57, medium category vs 0.29, low category). The mastery level achievement in the experimental group (92.60%) was substantially higher compared to the control group (66.70%). Superior performance can be attributed to enhanced peer interaction facilitating deeper conceptual understanding through social constructivism and the Zone of Proximal Development. These findings indicate that the peer tutoring method effectively enhances students' learning activity, motivation, and outcomes in complex biology topics, with practical implications for improving biology education quality at the secondary school level.
The Effect of Skul.id-Based Blended Learning with the Station Rotation Model on Students’ Perceptions, Activities, and Science Learning Outcomes at the Junior High School Andi Hasnah; Firdaus Daud; Oslan Jumadi; Yusminah Hala; Faisal Faisal
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.14312

Abstract

The Skul.id platform is a local Learning Management System (LMS) designed to support blended learning through features such as access to learning materials, assignment submission, and online evaluation. This study aimed to examine the effectiveness of Skul.id-based blended learning with the station rotation model on students’ perceptions, learning activities, and science learning outcomes. A quasi-experimental method with a nonequivalent control group design was employed, involving 54 eighth-grade students. The experimental group received blended learning with station rotation, while the control group received blended learning without station rotation. Data were collected using questionnaires, observation sheets, and learning achievement tests. The results showed significant differences between the two groups. The experimental group demonstrated very positive perceptions (96.30%), very high learning activity (100%), and higher learning outcomes with a mean posttest score of 77.78 and an N-gain of 0.56. In contrast, the control group showed positive perceptions (77.78%), active learning activity (92.59%), and lower learning outcomes with a mean posttest score of 65.48 and an N-gain of 0.36. Although both N-gain values were categorized as moderate, the experimental group achieved a substantially higher improvement. Independent sample t-test results confirmed statistically significant differences in perceptions, learning activities, and learning outcomes (p < 0.05). These findings indicate that Skul.id-based blended learning with station rotation is more effective in improving students’ perceptions, learning activities, and science learning outcomes
Pengaruh Lembar Kerja Peserta Didik (LKPD) Berbantuan Video Animasi terhadap Kemampuan Pemecahan Masalah, Motivasi Belajar dan Hasil Belajar Kognitif Peserta Didik Dian Dwi Lestari; Yusminah Hala; Ismail; Firdaus Daud; Syamsiah
Jurnal Penelitian Pendidikan IPA Vol 12 No 4 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i4.14989

Abstract

This study aims to determine the effect of animated video-assisted student worksheets (LKPD) on problem-solving skills, learning motivation, and cognitive learning outcomes of eleventh-grade students at SMA Negeri 3 Palu. This study employed a quantitative method with a quasi-experimental design, with a non-equivalent pretest-posttest control group design. The population was all eleventh-grade science students at SMA Negeri 3 Palu, with a sample size of 72 students selected using purposive sampling. Data analysis was conducted using descriptive statistics analysis techniques to describe the effect of animated video-assisted LKPD through pretest and posttest data. Furthermore, inferential statistics analysis was conducted using analysis of covariance to test the research hypotheses. The results of descriptive analysis on each measured variable show that the experimental class taught using animated video-assisted student worksheets achieved higher scores than the control class. This is also proven through significant hypothesis testing on each variable, namely on problem-solving ability (p = 0.03), learning motivation (p < 0.01), and cognitive learning outcomes (p < 0.01). Through the results of this analysis, it can be concluded that animated video-assisted student worksheets have an effective effect in improving students' problem-solving abilities, learning motivation, and cognitive learning outcomes.
Co-Authors . Hasrah, . . Hijriah, . ., Hardiyanti ., Syafiuddin A. Irma Suryani A. Mushawwir Taiyeb A. Mushawwir Taiyeb, A. Mushawwir Abd Muis Adhytama S, Ryan Putra Adnan Adnan Adnan Adnan Ahmad Fudhail  Majid Ahqaf, Nurul Afifah Ainun Mardhiyah Akida Akida Alimuddin Ali Alimuddin Ali Am, Septian Dwi Cahyo. Amalia, Riska Dina Amelia, Nila Andi Asmawati Azis Andi Asmawati Aziz Andi Bida Purnamasari Andi Faridah Arsal Andi Hasnah Andi Mu’nisa Andi Nurfaizah Yusuf Andi Rahmat Saleh Andi Tenri Ola Rivai Andri Adi Mustika Andri Adi Mustika, Andri Adi Angraeni, Andi Tirta Anisa Oktobianti , Andi AnisahAmri, Huriyah Antonius Suwanto Aprilian Pailang, Agista Aqilah Fauziyah MF AR, Mira Arhamar, A. S. Muhammad Arifah Novia Arifin, Arifah Novia Arifin, Arifah Novia Armin Arif Arni Arni Arsad Bahri Asdaniar, Asdaniar Asham Bin Jamaluddin Asmirah, Nurul Asnir Andriani Usman Astuti S, Dewi Athirah Azis, Putri Atira, Aynun Azazi, Amalia Nur Citra Sakinah Dian Dwi Lestari Dirawan, Gufron D Elsa Erlin, Charisniaty Ernawati Ernawati Faisal Faisal Fatmawati, Fatmawati Firdaus Daud Firdaus Daud Fitriani Fitriani Gufran D. Dirawan Gufran D. Dirawan, Gufran D. Hadis, Abdul Hafsan Halifah Pagarra Hammado, Nururrahmah Hartati, A. Hartina Harjuna Hartono Hartono Hasan, Nur Haslinar Hasniar Hasniar, Hasniar Henriawang Henriawang Herlina Rante Hilda Karim I Ketut Suada Idris, Irma Suryani Ismail . Ismail Ismail Jamaluddin, Nurbina Septiani Jr., Muhammad Zairin Kadir, St. Fatima Khatimah, Khusnul Latif, Rahmawati Lodang, Hamka M. Nur, Risna Maghfirah, Yaumil Maghfirah Majid, Anna Marsa, Marsa Masrida, Masrida Mu'nisa, A. Muarifah, Muarifah Muh. Junda Muh. Wahyudi Jasman Muhammad Arsyad Muhammad Danial Muhammad Farid Muhammad Isrul Muhammad Junda Muhammad Wiharto Muhiddin Palennari Muis, Sahribulan Muliana Mulianti Muliyati M Mushawwir Taiyeb Muthmainna, Nur Muthmainnah, Thiara Adzkia Nani Kurnia Nani Kurnia, Nani Narsan, Zhalfariani Nia Febrianti Nur Aida, Nur Nur Fadila Harifa Nur Veryani, Andi Nur wahidah Nurhayati B Nurlia Nurlia Nurul Arini Nurul Fadhilah Nurul Muhlisa Oslan Jumadi Oslan Jumadi Pagarra, Halifah Patongai, Dian Dwi Putri Ulan Sari Rachmawaty Rachmawaty Muchtar Rachmawaty Muchtar, Rachmawaty Rachmawaty Rachmawaty, Rachmawaty Rachmawaty, Rachmawaty Ramadani, Rani Ramlawati Ramlawati Rauf, Asnaeni Razak, Muliana Resti Handayani, Resti RIDWAN AFFANDI Risma Syam Rosdiana Ngitung Ruli, Fitha Febrilia Sadriani, Andi Sahodding, Sahodding Sahribulan Saleh, Andi Rahmat Samad, Hamidah Saniah, St Sirniawan, Sirniawan Sitti Saenab Sitti Saenab, Sitti Sri Wahyuni St. Fatmah Hiola Suhartini Azis Suradi Tahmir, Suradi Suriyanto Syahriani Syamsiah Syamsiah Syamsiah, Syamsiah Syamsu Rijal Syamsul Syamsul Syamsul Tahmir, Suradi Taiyeb A. Mushawwir Taiyeb, Andi Mushawwir Tenri Ampa Nurfitria Papada, Andi Tenri Lalla, Putri Pati Todingan, Ajeng Klarica Wahidatul Munandar Widiana, Riska Dina Wiji Astuti, Wiwik Wirna Arsyad Wirna Arsyad Wulandari R, Wulandari R Yeheskiel, Wini Yuliana Yuliana Yundari, Yundari Yusnaeni Yusuf Yusuf, Syahriani Yusuf, Yusnaeni Zulkarnaen Zulkarnaen