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Sytematic Literature Review: Implementasi Teknologi CRISPR/dCas9 untuk Memperkaya Nutrisi Tanaman melalui Modifikasi Jalur Metabolisme Narsan, Zhalfariani; AnisahAmri, Huriyah; Hala, Yusminah
BIO-SAINS : Jurnal Ilmiah Biologi Vol. 6 No. 1 (2026): Bio-Sains Vol. 6 No. 1, Maret 2026
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/1fxffe10

Abstract

Biofortifikasi tanaman merupakan strategi penting untuk meningkatkan kandungan mikronutrien dan senyawa fungsional guna mendukung ketahanan pangan berkelanjutan. Pendekatan konvensional sering kali terbatas dalam mengatur jalur metabolisme yang kompleks dan melibatkan banyak gen. Artikel ini bertujuan untuk mengidentifikasi, menyeleksi, dan mensintesis bukti ilmiah terkini mengenai implementasi teknologi CRISPR/dCas9 (CRISPRa, CRISPRi, dan pendekatan epigenetik) dalam modifikasi jalur metabolisme untuk memperkaya nutrisi tanaman. Penelitian dilakukan menggunakan metode Systematic Literature Review (SLR) berbasis PRISMA terhadap artikel terindeks Scopus periode 2022–2026. Hasil kajian menunjukkan bahwa CRISPR/dCas9 mampu mengatur ekspresi gen secara presisi tanpa pemotongan DNA, baik melalui aktivasi, represi, maupun fine-tuning epigenetik. Strategi multipleks memungkinkan pengendalian beberapa gen dalam satu lintasan biosintesis untuk mengoptimalkan metabolic flux dan meningkatkan produksi metabolit bernilai nutrisi. Selain itu, pendekatan epigenetik berbasis dCas9 membuka peluang regulasi gen yang lebih fleksibel tanpa perubahan permanen pada sekuens genom. Meskipun demikian, tantangan seperti efek off-target, keterbatasan sistem pengiriman, dan stabilitas transgenerasional masih memerlukan optimalisasi lebih lanjut. Secara keseluruhan, CRISPR/dCas9 berpotensi menjadi teknologi biofortifikasi presisi yang mendukung pengembangan pertanian modern dan berkelanjutan.
DREB1 Gene in Regulating Drought Tolerance in Cereal Plants Todingan, Ajeng Klarica; Aprilian Pailang, Agista; Hala, Yusminah
Bioeksperimen: Jurnal Penelitian Biologi March 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10172

Abstract

This study aims to explain the role of the DREB1 gene in increasing drought tolerance in plants with an emphasis on recent advances in genetic engineering. In this research, the method used is a literature review by collecting library data, reading and taking notes, and critically managing research data related to the DREB1 gene. In this research, data analysis starts by examining the results from studies that are most relevant to the research problem. The results of the literature review obtained indicate that the DREB1 gene is a transcription factor that plays a role in regulating a number of other genes related to drought characteristics. The DREB genes participate in hormone signalling pathways and regulate proteins that interact with downstream genes to improve plant stress tolerance. When there is a drought, seedlings that have the DREB gene inserted have a survival rate of 36%, while plants without the DREB gene all die. These results indicate that the DREB1 gene is a positive regulatory gene for heat tolerance in plants. In this case, the transgenic lines keep their stomata partially closed, which helps maintain a minimum transpiration rate to cool the canopy and reduce the temperature of both the canopy and leaves. We achieve this by coordinating the control of stomata, minimising water loss, and preserving cell turgor, all of which prevent the plant from dying.
Enhancing Student Motivation and Learning Outcomes Through Teams Games Tournament Integration with Wordwall Wahidatul Munandar; Yusminah Hala; Syamsiah Syamsiah
Journal of Innovation and Research in Primary Education Vol. 5 No. 1 (2026)
Publisher : Papanda Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56916/jirpe.v5i1.2644

Abstract

This quasi-experimental study investigated the differential effects of Teams Games Tournament (TGT) learning model with and without Wordwall integration on eleventh-grade biology students' motivation and learning outcomes at SMAN 8 Gowa, Indonesia. Seventy-two students were assigned to two experimental groups: R1 (TGT with Wordwall, n=36) and R2 (TGT only, n=36). Data were collected using a 20-item motivation questionnaire and a 30-item achievement test administered as pretests and posttests. Statistical analyses included descriptive statistics, one-way ANOVA, and normalized gain (N-Gain) calculations. Results revealed significant differences favoring TGT-Wordwall integration for both motivation (F(1,70)=38.42, p<.001, η²=.354) and learning outcomes (F(1,70)=86.77, p<.001, η²=.554). The R1 group achieved high N-Gain scores (g=0.78 for motivation; g=0.82 for learning outcomes) compared to R2's moderate gains (g=0.50 and g=0.63 respectively). Categorical analysis showed 66.7% of R1 students reached adequate or good performance levels versus only 8.3% in R2. These findings demonstrate that integrating interactive digital platforms with cooperative learning models substantially amplifies instructional effectiveness. The study provides empirical evidence supporting technology-enhanced pedagogy in secondary education and offers practical implications for educators implementing 21st-century learning approaches in biology instruction.
Predicting Biology Identity from Plant Literacy, Systems Thinking, and Reflective Judgment: A PLS-SEM Approach Ismail Ismail; Yusminah Hala; Nurhayati B; Suhartini Azis; Asham Bin Jamaluddin
International Journal of Educational Practice and Policy Vol. 4 No. 1 (2026): March-April 2026
Publisher : PT. Global Research Collaboration

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.66314/ijepp.v4i1.418

Abstract

This study examined the relationships among plant literacy, systems thinking, reflective judgment, and biology identity among university students. Using a quantitative non-experimental approach with a cross-sectional survey design, data were collected in January 2026 from students of the Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar. The data were obtained through a structured questionnaire using a 1–7 Likert scale and analyzed with Partial Least Squares Structural Equation Modeling (PLS-SEM). The results showed that the measurement model met the required reliability and validity criteria, with all indicator loadings above 0.70, AVE values above 0.50, and HTMT values below 0.90. In the structural model, plant literacy had a positive and significant effect on biology identity (β = 0.603, p < 0.001), and reflective judgment also had a positive and significant effect (β = 0.275, p < 0.001). In contrast, systems thinking had a negative but non-significant effect (β = -0.026, p = 0.647). The model explained 68.6% of the variance in biology identity. These findings indicate that biology identity is more strongly associated with students’ connection to core biological content and reflective engagement with knowledge than with systems thinking as a direct predictor.
The Effect of Android-Based Digital Poster Learning Media on Students' Digital Literacy in Ecosystem Suriyanto; Yusminah Hala; A. Mushawwir Taiyeb
Jurnal Penelitian Pendidikan IPA Vol 11 No 6 (2025): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i6.11495

Abstract

This research is motivated by the low digital literacy of biology of class X students of SMA Negeri 2 Luwu Timur. This study aims to determine the effect of android-based digital poster learning media on students' digital literacy in ecosystem material. This research is a quasi-experimental study with a Nonequivalent Control Group Design research design. The population of this study was all class X with a sample of 2 classes selected randomly. Which consists of 1 experimental class taught using android-based digital poster media, and 1 control class taught using wordwall-based open the box media. Data collection using a questionnaire given before and after treatment in the experimental class and control class. The results of the hypothesis testing at the digital literacy level showed a significance value of 0.023. This shows a sig value <α (0.05) so it can be concluded that H0 is rejected and H1 is accepted. This means that there is a difference in the influence of digital literacy of students taught using Android-based Digital Poster learning media and wordwall-based Open the Box learning media in class X Ecosystem material.
Peer Tutoring for Sustainable Biology Education: Enhancing Student Activity, Motivation, and Learning Outcomes in the Respiratory System Unit Nurul Arini; Yusminah Hala; Rachmawaty; Firdaus Daud; Ismail
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.14096

Abstract

This study aims to analyze the effect of the peer tutoring method on learning activity, learning motivation, and biology learning outcomes of grade XI students at SMAN 15 Gowa. This quasi-experimental research employed a pretest-posttest control group design involving 54 students divided into experimental (n = 27) and control groups (n = 27). Learning activity was measured using observation sheets based on Paul B. Diedrich indicators, motivation was assessed through questionnaires using Keller's ARCS model, and learning outcomes were evaluated using cognitive tests consisting of 25 multiple-choice and 5 essay questions based on revised Bloom's taxonomy. Data were analyzed using Independent Samples t-test to compare groups and Paired Samples t-test to analyze improvements within groups, with a significance level of α = 0.05. The results showed significant effects of peer tutoring on learning activity (p = 0.015), motivation (p = 0.000), and learning outcomes (p = 0.000). The experimental group demonstrated higher mean scores in activity (25.93 vs 24.44) and substantial improvements in motivation (N-Gain 0.61, medium category vs 0.12, low category) and learning outcomes (N-Gain 0.57, medium category vs 0.29, low category). The mastery level achievement in the experimental group (92.60%) was substantially higher compared to the control group (66.70%). Superior performance can be attributed to enhanced peer interaction facilitating deeper conceptual understanding through social constructivism and the Zone of Proximal Development. These findings indicate that the peer tutoring method effectively enhances students' learning activity, motivation, and outcomes in complex biology topics, with practical implications for improving biology education quality at the secondary school level.
The Effect of Skul.id-Based Blended Learning with the Station Rotation Model on Students’ Perceptions, Activities, and Science Learning Outcomes at the Junior High School Andi Hasnah; Firdaus Daud; Oslan Jumadi; Yusminah Hala; Faisal Faisal
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.14312

Abstract

The Skul.id platform is a local Learning Management System (LMS) designed to support blended learning through features such as access to learning materials, assignment submission, and online evaluation. This study aimed to examine the effectiveness of Skul.id-based blended learning with the station rotation model on students’ perceptions, learning activities, and science learning outcomes. A quasi-experimental method with a nonequivalent control group design was employed, involving 54 eighth-grade students. The experimental group received blended learning with station rotation, while the control group received blended learning without station rotation. Data were collected using questionnaires, observation sheets, and learning achievement tests. The results showed significant differences between the two groups. The experimental group demonstrated very positive perceptions (96.30%), very high learning activity (100%), and higher learning outcomes with a mean posttest score of 77.78 and an N-gain of 0.56. In contrast, the control group showed positive perceptions (77.78%), active learning activity (92.59%), and lower learning outcomes with a mean posttest score of 65.48 and an N-gain of 0.36. Although both N-gain values were categorized as moderate, the experimental group achieved a substantially higher improvement. Independent sample t-test results confirmed statistically significant differences in perceptions, learning activities, and learning outcomes (p < 0.05). These findings indicate that Skul.id-based blended learning with station rotation is more effective in improving students’ perceptions, learning activities, and science learning outcomes
Pengaruh Lembar Kerja Peserta Didik (LKPD) Berbantuan Video Animasi terhadap Kemampuan Pemecahan Masalah, Motivasi Belajar dan Hasil Belajar Kognitif Peserta Didik Dian Dwi Lestari; Yusminah Hala; Ismail; Firdaus Daud; Syamsiah
Jurnal Penelitian Pendidikan IPA Vol 12 No 4 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i4.14989

Abstract

This study aims to determine the effect of animated video-assisted student worksheets (LKPD) on problem-solving skills, learning motivation, and cognitive learning outcomes of eleventh-grade students at SMA Negeri 3 Palu. This study employed a quantitative method with a quasi-experimental design, with a non-equivalent pretest-posttest control group design. The population was all eleventh-grade science students at SMA Negeri 3 Palu, with a sample size of 72 students selected using purposive sampling. Data analysis was conducted using descriptive statistics analysis techniques to describe the effect of animated video-assisted LKPD through pretest and posttest data. Furthermore, inferential statistics analysis was conducted using analysis of covariance to test the research hypotheses. The results of descriptive analysis on each measured variable show that the experimental class taught using animated video-assisted student worksheets achieved higher scores than the control class. This is also proven through significant hypothesis testing on each variable, namely on problem-solving ability (p = 0.03), learning motivation (p < 0.01), and cognitive learning outcomes (p < 0.01). Through the results of this analysis, it can be concluded that animated video-assisted student worksheets have an effective effect in improving students' problem-solving abilities, learning motivation, and cognitive learning outcomes.
The Effect of Project-Based Learning (PjBL) with STEM Approach on Students’ Metacognitive Awareness, Conceptual Understanding, and Biology Problem-Solving Ability Nia Febrianti; Firdaus Daud; Yusminah Hala; Muhiddin Palennari; Nurhayati B
Jurnal Penelitian Pendidikan IPA Vol 12 No 4 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i4.15006

Abstract

This study aims to analyze effect of the Project-Based Learning model based on the STEM approach on metacognitive awareness, conceptual understanding, and biology problem-solving abilities of grade XI students of SMA Negeri 13 Bone. The novelty of this study lies in integration of PjBL-STEM which simultaneously measures three high-level cognitive aspects in one experimental design. This study is a quasi-experimental study with a Pretest-Posttest Nonequivalent Control Group Design. The sample consisted of two classes (n=72) using a purposive sampling technique. Instruments included the MAI questionnaire, an essay test based on the SOLO taxonomy (prestructural to expanded abstract), and a problem-solving test based on Polya's stages, all of which have met high validity and reliability. Data were analyzed using ANCOVA (α = 0.05). The results showed that PjBL-STEM had a significant effect on metacognitive awareness (p=.004), conceptual understanding (p=.01), and biology problem-solving ability (p<.001). The experimental group showed a higher average increase compared to the control, indicating the effectiveness of PjBL-STEM in enhancing systematic and reflective thinking to support development of 21st century skills. Limitations of this study include quasi-experimental design without full randomization and sample size limited to one school. Further research is needed to test the generalizability of the findings.
The Relationship between Metacognitive Awareness, Self-Efficacy and Learning Motivation on Biology Learning Outcomes for Class XI State High School Students in Soppeng Regency Wirna Arsyad; Muhiddin Palennari; Alimuddin Ali; Firdaus Daud; Yusminah Hala
Jurnal Penelitian Pendidikan IPA Vol 12 No 5 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i5.15117

Abstract

This study aims to analyze the relationship between metacognitive awareness, self-efficacy, and learning motivation on biology learning outcomes of eleventh-grade students in public high schools in Soppeng Regency, both directly and indirectly. This quantitative study employed an ex post facto correlation approach with Structural Equation Modeling (SEM) analysis techniques. A sample of 505 students was selected using simple random sampling and proportional sampling techniques from a total population of 1.444 students. The instruments used included questionnaires on metacognitive awareness, self-efficacy, and learning motivation, as well as a biology learning achievement test. The results showed that metacognitive awareness was significantly related to learning motivation (p=0.00), but had no direct effect on learning outcomes (p=0.06). Self-efficacy was significantly related to learning outcomes (p=0.01), but not significantly to learning motivation (p=0.06). Learning motivation was also significantly related to learning outcomes (p=0.00). Indirectly, metacognitive awareness has a significant relationship with learning outcomes through the mediation of learning motivation (p=0.00) and self-efficacy (p=0.02). However, the indirect relationship between self-efficacy and learning outcomes through learning motivation was not significant (p=0.16). The conclusion of this study confirms that learning motivation plays a crucial role as a primary mediator. Increasing metacognitive awareness and self-efficacy needs to be optimized in an integrated manner to improve student learning outcomes.
Co-Authors . Hasrah, . . Hijriah, . ., Hardiyanti ., Syafiuddin A. Irma Suryani A. Mushawwir Taiyeb A. Mushawwir Taiyeb, A. Mushawwir Abd Muis Adnan Adnan Adnan Adnan Ahmad Fudhail  Majid Ainun Mardhiyah Akida Akida Alimuddin Ali Alimuddin Ali Am, Septian Dwi Cahyo. Amalia, Riska Dina Amelia, Nila Andi Asmawati Azis Andi Asmawati Aziz Andi Bida Purnamasari Andi Faridah Arsal Andi Hasnah Andi Mu’nisa Andi Mu’nisa Andi Nurfaizah Yusuf Andi Rahmat Saleh Andi Tenri Ola Rivai Andri Adi Mustika Andri Adi Mustika, Andri Adi Angraeni, Andi Tirta Anisa Oktobianti , Andi AnisahAmri, Huriyah Antonius Suwanto Aprilian Pailang, Agista Aqilah Fauziyah MF AR, Mira Arhamar, A. S. Muhammad Arifah Novia Arifin, Arifah Novia Arifin, Arifah Novia Arni Arni Arsad Bahri Asdaniar, Asdaniar Asham Bin Jamaluddin Asmayani Asmirah, Nurul Asnir Andriani Usman Astuti S, Dewi Atira, Aynun Azazi, Amalia Nur Citra Sakinah Dian Dwi Lestari Dirawan, Gufron D Elsa Erlin, Charisniaty Ernawati Ernawati Faisal Faisal Fatmawati, Fatmawati Firdaus Daud Firdaus Daud Fitriani Fitriani Gufran D. Dirawan Gufran D. Dirawan, Gufran D. Gufran D.Dirawan Hadis, Abdul Hafsan Halifah Pagarra Hamidah Samad Hammado, Nururrahmah Hartati, A. Hartina Harjuna Hartono Hartono Hasniar Hasniar, Hasniar Henriawang Henriawang Herlina Rante Hilda Karim I Ketut Suada Idris, Irma Suryani Imelia Ada Ismail . Ismail Ismail Jamaluddin, Nurbina Septiani Khatimah, Khusnul Latif, Rahmawati Lodang, Hamka M. Nur, Risna Majid, Anna Marsa Marsa, Marsa Masrida, Masrida Mu'nisa, A. Muh. Junda Muh. Wahyudi Jasman Muhammad Arsyad Muhammad Danial Muhammad Farid Muhammad Isrul Muhammad Junda Muhammad Wiharto MUHAMMAD ZAIRIN Jr. Muhiddin Palennari Muis, Sahribulan Muliana Muliana Razak Mulianti Muliyati M Mushawwir Taiyeb Muthmainna, Nur Muthmainnah, Thiara Adzkia Nani Kurnia Nani Kurnia, Nani Narsan, Zhalfariani Nia Febrianti Nur Aida, Nur Nur Fadila Harifa Nur Haslinar Hasan Nur Veryani, Andi Nur Wahidah Nurhayati B Nurlia Nurlia Nurul Afifah Ahqaf Nurul Arini Nurul Fadhilah Nurul Muhlisa Oslan Jumadi Oslan Jumadi Pagarra, Halifah Patongai, Dian Dwi Putri Ulan Sari Pujiati Rachmawaty Rachmawaty Muchtar Rachmawaty Muchtar, Rachmawaty Rachmawaty Rachmawaty, Rachmawaty Rachmawaty, Rachmawaty Ramadani, Rani Ramlawati Ramlawati Rani Ramadani Rauf, Asnaeni Razak, Muliana Resti Handayani, Resti RIDWAN AFFANDI Rifka Annisa Risma Syam Rosdiana Ngitung Ruli, Fitha Febrilia Sadriani, Andi Sahodding Sahodding Sahribulan Saleh, Andi Rahmat Saniah, St Sirniawan, Sirniawan Sitti Saenab Sitti Saenab, Sitti Sri Wahyuni St. Fatima Kadir St. Fatmah Hiola Suhartini Azis Suradi Tahmir, Suradi Suriyanto Syahriani Syahriani Yusuf Syamsiah Syamsiah Syamsiah, Syamsiah Syamsul Syamsul Syamsul Tahmir, Suradi Taiyeb A. Mushawwir Taiyeb, Andi Mushawwir Tenri Ampa Nurfitria Papada, Andi Tenri Lalla, Putri Pati Todingan, Ajeng Klarica Ulfa Auliah Dwiyanti Wahidatul Munandar Widiana, Riska Dina Wiji Astuti, Wiwik Wirna Arsyad Wirna Arsyad Wulandari R, Wulandari R Yeheskiel, Wini Yuliana Yuliana Yundari, Yundari Yusnaeni Yusuf Yusuf, Yusnaeni Zulkarnaen Zulkarnaen