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AT3 (Acyltransferase) Gene Isolated From Capsicum frutescens cv. Cakra Hijau Habibi, Mohamad; Manggabarani, Andi Madhihah; Sulasmi, Eko Sri; Listyorini, Dwi
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Chili pepper is widely used and cultivated by Indonesian people. There are three species of chili pepper, i.e.: Capsicum annuum L., Capsicum frutescens L., and Capsicum violaceum HBK. Capsicum frutescens L. has a higher economic value due to its pungency and carotenoid content. C. frutescens has several cultivars, one of those is Capsicum frutescens cv. Cakra Hijau. This cultivar is resistant against pest and disease and has very high pungency. This special character of chili pepper is born by its secondary metabolic, Capsaicin. Moreover, capsaicin also serves as defense mechanism, antiarthritis, analgesic, and anticancer. This study aimed to isolate Acyltransferase (AT3) gene which encoding Capsaicin Synthase (CS) enzyme. AT3 gene was isolated through PCR using forward primer 5-ATG GCT TTT GCA TTA CCA TCA-3 and reverse primer 5-CCT TCA CAA TTA TTC GCC CA-3. Data were analyzed using DNA Baser, BLAST, and ClustalX. This study has successfully isolated 404 bp fragments of AT3 gene. This fragments located at 1918-1434 bp referred to AT3 gene from Capsicum frutescens cv. Shuanla. Isolation of upstream and downstream fragments of AT3 gene from Capsicum frutescens cv. Cakra Hijau is undergoing.
Morphological Study for Identification Improvement of Poeciliidae Family Based on Gonopodium structures and Morphometric analysis Rahayu, Dwi Anggorowati; Listyorini, Dwi; Ibrohim, Ibrohim
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.2.%x

Abstract

Poeciliidae family consists of small live-bearer fishes with sexual dimorphic. This fishes are widely distributed across Indonesian archipelago, but biological studies on this fish are very limited. This research is aimed to identify the species of Peciliidae fishes which lives in Sari Lake Pasuruan based on gonopodium structures and morphometric analysis. A specific characteristic of the Poeciliidae family is its gonopodium structures which is a modification of 3rd, 4th, and 5th rays of the males anal fin. Each species represented 50 samples male and female from each type we found and measured 26 characters (in mm). Cladogram based on morphometric measurements showed that sample 1 and 2 had 100% similarity, sample 3 had 94% similarity with sample 1 and 2. Sample 4 had 54% similarity with sample 1, 2, and 3. Based on gonopodium structures sample 1 and sample 2 included Xiphophorus hellerii Heckel possess large claw on the terminus of ray 5 hook is small crescent-moon shaped on 3rd ray and lacking of gonopodium palp, sample 3 possesses distal platform with 11 retrosse serrae,and membranous hook so we were able to identify the sample 3 as Poecilia mexicana Steindachner, whilst sample 4 was Poecilia reticulata Peter had a long-pointed comb-like spines on ray 3rd and and has a small hook on the tip of 5p ray.
Observation of Frog Species in State University of Malang as a Preliminary Effort on Frog Conservation Wulandari, Dian Ratri; Habibi, Muhammad; Listyorini, Dwi
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.1.%x

Abstract

Frog is an amphibian which widely distributed around the world. Indonesia houses 450 species which represent 11% of frog species in the world. In Java Island living 42 species of frogs and toads. Frogs can be used as an environment indicator due to the presence of frog in a particular place indicates that the place is stay natural and unpolluted. State University of Malang Campus #1 which is located in the heart of Malang District has been developing rapidly, currently. Thus, it requires for the construction of new various facilities to support it widen activities. Extensive construction can be destructive even damaging to the habitat of frog, which potentially threat the frog life, if it does not take a consideration on the environmental impact, carefully. This study aimed to identify the species of frog which is survives in State University of Malang with consideration of the frog species found in 1995. Species identification conducted by observing the morphologicalcharacter. This study found four species with three species which are survive and as found in 1995; those are Duttaphrynus melanostictus, Polypedates leucomystax, and Kaloula baleta; and one new species that is Rana chalconota. This study also reveals there are four species which are extinct; those are Fejervarya cancrivora, Fejervarya limnocharis, Ingerophrynus biporcatus, and Occidoziga lima. This situation shows the decreasing amount of species from 7 to 4 within 17 years. This result indicates that there is a serious environmental degradation which caused the losing of frog habitats. Further research is needed to study the ecological condition changing in order to save the frog species.
Embryo Development of Tree Frog Polypedates leucomystax at Campus of State University of Malang Peralindah, Pearlindah; Kusumawati, Emy; Wulandari, Dian Ratri; Listyorini, Dwi
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Tree frogs live in natural places which are unpolluted. Regarding their role as an ecological indicator, the decrease of frogs population in a particular habitat indicates the danger of environment quality decrease. Moreover, this condition can harm the frogs themselves. All kinds of frogs breed in aqueous environment such as ponds, marshes, and farming fields. One of the tree frogs, Polypedates leucomystax, which belongs to Familia Rachophoridae, is widely spread in Indonesia. This frog has yellowish brown skin with black spots or six lines extending from head to the posterior tip of body. A breeding couple of the frog produces foam nests on the water or plants around water body, where they will nest their fertilized eggs. This species produces over a hundred embryos in one spawning season. These embryos require appropriate conditions to develop normally in the nature. Frog embryo development may becomes a reference to understand how the frog population survives. This study focused on P. leucomystax with regards to its decrease in number due to the drying up of the environment and a lot lost of trees in Campus of State University of Malang. The development of P. leucomystax embryos in the reproduction foam was observed until it reached a tadpole stage. The result showed that the embryos developed in the foam until they hatched then they move out of the foam into the water under which they would continue their development. Considering that water body is a critical requirement for the development of P. leucomystax embryos, it is our responsibility to make any efforts to conserve not only the trees but also any type of water bodies including ponds, marshes, and farming fields as well. Keywords: embryo development, Polypedates leucomystax, Tree frog conservation
Mutation Analysis and Single Nucleotide Polymorphism of TP53 Gene in Breast Cancer in East Java Radhiyah, Rizqa; Ardana, I Kade Karisma Gita; Wisnubroto, Wisnubroto; Listyorini, Dwi; Susanto, Hendra
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.12

Abstract

The Incidence of Indonesia breast cancer case in 2018 was reported at 20.7% or 160,653 in number. The factors that caused breast cancer is TP53 gene mutation and Single Nucleotide Polymorphism (SNP). This study aimed to determine the mutation and Single Nucleotide Polymorphism (SNP) of TP53 gene in breast cancer. Samples of this study were 9 people chosen based on a purposive technique. The methods include total DNA isolation, DNA quantification, PCR, and sequencing. The results of the sequencing were then analyzed using alignment and blast. The SNP is browsed by the SNP finder on NCBI both followed by protein modeling. The results of this study indicate the existence of mutation in the exon and intron regions. Substitution of Guanine (G) to base Adenine (A) is occur in codon 496 which is a coding region. SNP was also obtained in exon of sample 12. The conclusion of this study is breast cancer can be caused by mutations that occur in the exon and altered the structur of protein structure, so then affect the binding affinity between p53 and its target domain. Tp53 gene mutation is not the only cause of breast cancer staging development, there are other genes that also contribute to the development of cancer stage. In addition, Single Nucleotide Polymorphism in the TP53 gene can be used as a predisposition marker of breast cancer that has potential to be inherited
Haplotype Diversity of COI Gene of Hylarana chalconota Species Found at State University of Malang Wulandari, Dian Ratri; Ibrohim, Ibrohim; Listyorini, Dwi
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.1.%x

Abstract

Hylarana chalconota is a cryptic species of frog endemic to Java Island . This species is small with long legs, and brown skin. The Snout-Vent Length (SVL) ranges between 30-40 mm for male and 45-65 mm for female. Reports the existence of this species in State University of Malang, which was not found in 1995. To reveal the haplotype diversity of COI gene in this species, we analyzed Cytochrome-c oxidase subunit-1 (COI) sequences of both samples. The both samples had 604 bp and 574 bp fragment length, respectively. These fragments showed polymorphism; with mutation position in sites 104, 105, and 124. Based on this result, we suggest that the two samples share a different haplotypes, proposed as UM1 and UM2
Embryo Development of Tree Frog Polypedates leucomystax at Campus of State University of Malang Pearlindah Peralindah; Emy Kusumawati; Dian Ratri Wulandari; Dwi Listyorini
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tree frogs live in natural places which are unpolluted. Regarding their role as an ecological indicator, the decrease of frogs population in a particular habitat indicates the danger of environment quality decrease. Moreover, this condition can harm the frogs themselves. All kinds of frogs breed in aqueous environment such as ponds, marshes, and farming fields. One of the tree frogs, Polypedates leucomystax, which belongs to Familia Rachophoridae, is widely spread in Indonesia. This frog has yellowish brown skin with black spots or six lines extending from head to the posterior tip of body. A breeding couple of the frog produces foam nests on the water or plants around water body, where they will nest their fertilized eggs. This species produces over a hundred embryos in one spawning season. These embryos require appropriate conditions to develop normally in the nature. Frog embryo development may becomes a reference to understand how the frog population survives. This study focused on P. leucomystax with regards to its decrease in number due to the drying up of the environment and a lot lost of trees in Campus of State University of Malang. The development of P. leucomystax embryos in the reproduction foam was observed until it reached a tadpole stage. The result showed that the embryos developed in the foam until they hatched then they move out of the foam into the water under which they would continue their development. Considering that water body is a critical requirement for the development of P. leucomystax embryos, it is our responsibility to make any efforts to conserve not only the trees but also any type of water bodies including ponds, marshes, and farming fields as well. Keywords: embryo development, Polypedates leucomystax, Tree frog conservation
Observation of Frog Species in State University of Malang as a Preliminary Effort on Frog Conservation Dian Ratri Wulandari; Muhammad Habibi; Dwi Listyorini
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.1.%x

Abstract

Frog is an amphibian which widely distributed around the world. Indonesia houses 450 species which represent 11% of frog species in the world. In Java Island living 42 species of frogs and toads. Frogs can be used as an environment indicator due to the presence of frog in a particular place indicates that the place is stay natural and unpolluted. State University of Malang Campus #1 which is located in the heart of Malang District has been developing rapidly, currently. Thus, it requires for the construction of new various facilities to support it widen activities. Extensive construction can be destructive even damaging to the habitat of frog, which potentially threat the frog life, if it does not take a consideration on the environmental impact, carefully. This study aimed to identify the species of frog which is survives in State University of Malang with consideration of the frog species found in 1995. Species identification conducted by observing the morphologicalcharacter. This study found four species with three species which are survive and as found in 1995; those are Duttaphrynus melanostictus, Polypedates leucomystax, and Kaloula baleta; and one new species that is Rana chalconota. This study also reveals there are four species which are extinct; those are Fejervarya cancrivora, Fejervarya limnocharis, Ingerophrynus biporcatus, and Occidoziga lima. This situation shows the decreasing amount of species from 7 to 4 within 17 years. This result indicates that there is a serious environmental degradation which caused the losing of frog habitats. Further research is needed to study the ecological condition changing in order to save the frog species.
AT3 (Acyltransferase) Gene Isolated From Capsicum frutescens cv. Cakra Hijau Mohamad Habibi; Andi Madhihah Manggabarani; Eko Sri Sulasmi; Dwi Listyorini
Journal of Tropical Life Science Vol. 3 No. 2 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Chili pepper is widely used and cultivated by Indonesian people. There are three species of chili pepper, i.e.: Capsicum annuum L., Capsicum frutescens L., and Capsicum violaceum HBK. Capsicum frutescens L. has a higher economic value due to its pungency and carotenoid content. C. frutescens has several cultivars, one of those is Capsicum frutescens cv. Cakra Hijau. This cultivar is resistant against pest and disease and has very high pungency. This special character of chili pepper is born by its secondary metabolic, Capsaicin. Moreover, capsaicin also serves as defense mechanism, antiarthritis, analgesic, and anticancer. This study aimed to isolate Acyltransferase (AT3) gene which encoding Capsaicin Synthase (CS) enzyme. AT3 gene was isolated through PCR using forward primer 5-ATG GCT TTT GCA TTA CCA TCA-3 and reverse primer 5-CCT TCA CAA TTA TTC GCC CA-3. Data were analyzed using DNA Baser, BLAST, and ClustalX. This study has successfully isolated 404 bp fragments of AT3 gene. This fragments located at 1918-1434 bp referred to AT3 gene from Capsicum frutescens cv. Shuanla. Isolation of upstream and downstream fragments of AT3 gene from Capsicum frutescens cv. Cakra Hijau is undergoing.
Morphological Study for Identification Improvement of Poeciliidae Family Based on Gonopodium structures and Morphometric analysis Dwi Anggorowati Rahayu; Dwi Listyorini; Ibrohim Ibrohim
Journal of Tropical Life Science Vol. 3 No. 2 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.2.%x

Abstract

Poeciliidae family consists of small live-bearer fishes with sexual dimorphic. This fishes are widely distributed across Indonesian archipelago, but biological studies on this fish are very limited. This research is aimed to identify the species of Peciliidae fishes which lives in Sari Lake Pasuruan based on gonopodium structures and morphometric analysis. A specific characteristic of the Poeciliidae family is its gonopodium structures which is a modification of 3rd, 4th, and 5th rays of the males anal fin. Each species represented 50 samples male and female from each type we found and measured 26 characters (in mm). Cladogram based on morphometric measurements showed that sample 1 and 2 had 100% similarity, sample 3 had 94% similarity with sample 1 and 2. Sample 4 had 54% similarity with sample 1, 2, and 3. Based on gonopodium structures sample 1 and sample 2 included Xiphophorus hellerii Heckel possess large claw on the terminus of ray 5 hook is small crescent-moon shaped on 3rd ray and lacking of gonopodium palp, sample 3 possesses distal platform with 11 retrosse serrae,and membranous hook so we were able to identify the sample 3 as Poecilia mexicana Steindachner, whilst sample 4 was Poecilia reticulata Peter had a long-pointed comb-like spines on ray 3rd and and has a small hook on the tip of 5p ray.
Co-Authors @Mustaffa, Madiawati Mamat Abdul Ghofur Abdul Gofur Abdul Gofur Abdul Gofur Achmad Rodiansyah Afrabiyas Firyal Hanasepti Agung Andana Agung Andana Agung Wibowo Agung Witjoro Ainul Fitria Mahmudah Aisyah Hidayati Algafari Bakti Manggara Alvian Guntur Prasetya Aziz Alvian Guntur Prasetya Aziz Anastacia Verena Salim Andi Madhihah Manggabarani Anggraini, Nina Bunga Anggun Sari Anjarwati Annasa Sabatia Ardana, I Kade Karisma Gita Arini, Kartika Ayu Dwi Assegaff, Muhamad Farid Aswan Aswan Atik Kurniawati Awalin, Hafidzatuz Zuhriatin Azizatuz Zahro Badri'ah, Badri'ah Bani Sudardi Caesar Adlu Hakim Caesar Adlu Hakim Choirun Nita Fikriani Choirun Nita Fikriani Cicik Tri Jayanti Dahlia Dahlia Dahlia Dahlia Darmawan, Taufik Delia Wahyu Pangesti Deni Ainur Rokhim Dermawan, Taufik DEWI ARIANI Dewi Ariani Dhikri, Ahmad Saiqu Dhion Meitreya Vidhiasi Dian Ratri Wulandari Didin Widyartono Djoko Saryono Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwita Novitasari Dyati Galuh Pratita Effendi, Desy Irafadillah Eko Agus Suyono Eko Sri Sulasmi Elhah Nailul Khasna Emy Kusumawati Endang Suarsini Endik Deni Nugroho Endriawati, Riskha Anggita Erry Hidayanto Evania Yafie Evi Susanti Fadhillah, Rayhan Rizky Fahriani, Dita Karisma Farida, Rully Fatchur Rohman Fauziah, Putri Nanda Febrianto, Aziza Restu Fikriani, Choirun Nita Firnanda, Sindy Galina Istiqhfarini GAYUH ELPANUSI, GAYUH ELPANUSI Gracela Noveline Christiana Telaumbanua Habibi, Mohamad Hadi Sowono Hadi Suwono Haryani Haryani Hasbi, Nur Hayuni Retno Widarti Helmi Muzaki Hendra Susanto Herawati Susilo Herdina Rahma Zalita Hikam, Ahmad Faruqil Huda, Marleni Rosalia Ndapa Hudayanto, Reynaldi Januar I Kade Karisma Gita Ardana I Kade Karisma Gita Ardana I Kade Karisma Gita Ardana Ibrohim Iin Murtini Ike Ratnawati Indra Kurniawan Saputra Intan Chairun Nisa Ira Junita Suryantoro Iza, Nikmatul Izdihara Arini Aulia Jamila Wijayanti Jayawarsa, A.A. Ketut Jeni Jeni Jiilan Hani Safitri JK, Adi Kus Rochman Juliandani, Fasha Jumain, Jumain Ken Raos Regina Khamdilah, Ali Kuncoro, Condro Ayu Mukti Kurniawan Setia Putra Kusubakti Andajani Kusumawati, Emy Laili, Rani Nuzulul Laily Abida Lathif, Yudrik Lathif, Yudrik Lea Assyifa Lestari, Nunik Martin M. Miftahul Aziz M. Nidhamul Maulana Madiawati Binti Mamat Manggabarani, Andi Madhihah Marestya, Alfina Dwi Maria Goretti Indah Della Conseta Mastika Marisahani Ulfah Mauluddin, Imam Mitra Istiar Wardhana Moh. Safii Mohamad Habibi Mona Lisa Mona Lisa, Mona Muh Ade Artasasta Muhammad Habibi Muharramah, Disa Hijratul Muhibbatul Ma'rifah Mulyani, Dita Tri Munika, Risa Dewi Murni Sapta Sari Muslim Mustayah ., Mustayah Muthia Naila Mazieda Naailatu Nur Azizah Natasya, Shella NIKMATUL IZA Nikmatul Iza Nila Kartika Sari Nila Kartika Sari Nina Bunga Anggraini Ningsih, Husdiani Norman Yoshi Haryono Nur Hasbi Nurastuti, Galuh Yuli Nurjannah, Andhika Afifah Nurul Annisa Putri Nurul Annisa Putri Pearlindah Peralindah Peralindah, Pearlindah Pradipta, Dristanta Setya Prasetyani, Rizki Nurul Prima Vidya Asteria Puguh Darmawan Pujiyanto, Fajar Putra, Kurniawan Setia Putra, Kurniawan Setia Putri, Aldila Rahmita Putri, Cindy Pradina Putri, Nurul Annisa Raden Bambang Sumarsono Radhiyah, Rizqa Rahmawati, Yusi Rahmawati, Yusi Rahmi Masita Ramadhan, Aprika Agung Ratna Juwita Rayhan Rizki Fadhillah Ria Novita Ayu Komalasari Rina Wahyuningsih Riri Wiyanti Retnaningtyas Riri Wiyanti Retnaningtyas Rismiawati, Riris Risqillah, Ulfa Rizki Nurul Prasetyani Rizkian, Selvi Rizqa Radhiyah Rizqullah Janitra Kristian Abadi Rofi'ah, Ndzani Latifatur Rohmah, Riska Nur Rohmawati, Uun Rozana, Kennis Sari, Nila Kartika Setiawan, Nur Candra Eka Setyowati, Istri Sholekah, Reeno Al Hikmatus Sholihah, Mar'atus Siti Salina Mustakim Siti Zubaidah Siti Zubaidah Sitoresmi Prabaningtyas Sri Sukaesih Suharti Suharti Suharti Sukiman Sunarmi Susriyati Mahanal Sutejo Sutejo Sutejo Sutejo Tamara, Widia Tita Putri Milasari Triutami, Dwinisari Tunggal Mutika, Winnie Tutut Indria Permana Umrotin, Emilia Utami Sri Hastuti Wahyudi Siswanto Wahyudi Siswanto Wahyuningtyas, Afifah Fitri Wibowo, Rachmat Ari Wibowo, Rahmat Ari Wigrahanto, Kifan Wilastari, Santhi Winda Dwi Astuti Windri Hermadhiyanti Winnie Tunggal Mutika Wisnubroto Wisnubroto Wisnubroto, Wisnubroto Wulandari, Dian Ratri Yessi Affriyenni Yudrik Lathif Zelen Surya Minata Zidan Aqillah Rasyid Zufahmi Zufahmi Zuhairi, Aminudin Zulfia, Firda Ama ‘Ainun Sayyidah Zakiyah