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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Widyariset Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Indonesian Journal of Tropical Aquatic Widyariset Qadauna: Jurnal Ilmiah Mahasiswa Hukum Keluarga Islam Jurnal Media Akuakultur Indonesia Jurnal Pengabdian Inovasi dan Teknologi Kepada Masyarakat Jurnal Kedokteran Hewan International Journal of Educational and Life Sciences (IJELS) Journal of Fisheries & Marine Biodiversitas Journal of Biological Diversity

Alimuddin

Department Of Aquaculture, Faculty Of Fisheries And Marine Science, IPB University, Bogor, West Java, Indonesia

Author-ID : 2471926

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Performance of Nile tilapia juvenile fed diet containing different dosages ofrecombinant common carpgrowth hormone Hardiantho, Dian; Alimuddin, .; Prasetiyo, Arief Eko; Yanti, Dwi Hany; Sumantadinata, Komar
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The studywas conductedtodetermine thedose of recombinant common carp growth hormone (rCcGH) supplemented in the commercial diet that generates the best performanceonbodyweight, biomass, andfeed conversion rate of juvenile tilapia.The dose of rCcGH administered was10, 20, and30mg/kg of commercial feed, andnorGH supplementation as control. Eachtreatment was designed in triplicates. Juveniles at average bodylengthof about2cm(average body weight of 0.7 g) were reared in a density of25fish/m2, for three weeks in hapa net at a size of 2×2×1 m3. Fish were fed diet containing rCcGH twicea week with an interval ofthreedays. The juvenile were fed with the rCcGH supplemented diet three times daily at satiation. The results showedthat average body weight andbiomass of fish treated by rCcGH with the doses of 20 and 30 mg/kg feed was higher (p<0.05) than that of 10 mg/kg and control. In addition, feed conversionrate of 20 and 30 mg/kg feed treatments was lower (p<0.05) than that of 10 mg/kg and control. RT-PCR analysisshowedthat expression level of IGF-1 gene in juvenile liver was increased in parallel with the increasing of rCcGH dosages supplemented in diet.This suggestedthatIGF-1 plays an important rolein the induction ofgrowth,andfeed conversionefficiencyof tilapiafed diet containing rCcGH. The bestperformance of juvenile can be obtainedby feeding with diet containingrCcGH20‒30mg/kgof feed. Keywords: rCcGH, IGF-I, recombinant growthhormone,performance, Nile tilapia.

Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone Syazili, Aras; Irmawati, .; Alimuddin, .; Sumantadinata, Komar
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study was aimed to determine the immersion frequency of recombinant growth hormone (rGH) in the growth stimulation of giant gourami juvenile. A total of 50 larvae at second day after first feeding, was immersed in 30 ppt salt water for 2 minutes, and then transferred into 200 mL of 9 ppt salt water containing 30 mg/L rGH in inclusion bodies form. Immersion was performed for 60 minutes and repeated once a week for two, three, four, and five weeks. As the control, fish was immersed with inclusion bodies without rGH (control-1), bovine serum albumin (control-2), 9ppt saline water without rGH (control-3) and in freshwater (control-4). Fish were kept in 7L aquaria during the first four weeks, transferred into 50L aquaria for two weeks, and then in hapa (2×1×1 m3 in size) for two weeks. The results showed that the highest (p<0.05) average body weight of giant gourami juvenile (2.23±0.19 g) was obtained at four times (four weeks) immersion treatment. Similar body weight of fish in two times (1.72±0.29 g), three times (1.68±0.34 g), and five times (1.81±0.17 g) immersion was obtained. Fish body weight of all controls was lower than the rGH-immersed fish. Average body weight of four time rGH-immersed fish (4.01 g) remained higher than other treatment and control (1.96 to 2.36 g) on 8th week of fish rearing. Survival of four times (96.67±1.15%), and five times rGH-immersion fish (95.33±4.16%) was similar, and higher than other treatments and controls (77.33 to 89.33%). Thus, higher growth rate and survival of giant gourami juvenile could be achieved by four times rGH immersion. Keywords: recombinant protein, growth hormone, immersion frequency, giant gourami

Efficacy of DNA vaccine encoding koi herpesvirus glycoprotein GP-25in common carp juvenile by immersion Nuswantoro, Soko; Alimuddin, .; Yuhana, Munti; Santika, Ayi; Nuryati, Sri; Zainun, Zakki; Mawardi, Mira
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Koi herpesvirus (KHV) is a herpesvirus that particularly infects and causes mass mortality to koi and common carp. Therefore, the protection of common carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine encoding KHV glycoprotein-25 by immersion method to increase survival of common carp against KHV infection. A total of 400 common carp juveniles at 30-day-old were immersed in 1-L water containing 1.3×108CFU/mL of the killed Escherichia coli cells carrying DNA vaccine. Three frequencies and three duration of fish immersion were tested, namely: 1×30 minutes, 1×60 minutes, 1× 90 minutes, 2×90 minutes and 3×90 minutes by interval of 24 hours. Reversetranscription polymerase chain reaction analysis showed that DNA vaccine was successfully expressed in the vaccinated fish. Fish at twenty eight days post vaccination were challenged by injecting 10-4 mL of KHV per fish. The result showed that vaccination by 1×30 minutes immersion allowed 61% of fish survived, and this was significantly higher (p<0.05) compared to control (without vaccination), but it was similar among vaccination treatments (p>0.05). The relative percent survival of vaccinated fish were also similar among treatments (p>0.05). DNA vaccination has increased fish survival about two fold higher compared to unvaccinated fish control (26.67%). Thus, DNA vaccination was effectively delivered by immersion for 1×30 minutes, and this technique can be useful to level up the resistance of common carp juveniles against KHV infection. Keywords: DNA vaccine, KHV, glycoprotein, immersion, common carp

Growth, feed conversion and retention, and proximate of eel juvenile treated by immersion of recombinant giant grouper growth hormone Handoyo, Boyun; Alimuddin, .; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study was aimed to determine the immersion dose of recombinant giant grouper growth hormone (rElGH) to increase growth of eel juvenile (glass eel). After shock salinity treatment (NaCl 3% for 2 min), glass eel were immersed in water containing 0.9% NaCl, 0.01% bovine serum albumin, and different of dose of rElGH (0, 0.12, 1.2, 12 and 120 mg/L). Glass eel were fed on blood worm for the first month, and commercial diet for the second month of rearing. Fish rearing was performed in 60 L glass aquarium at density of 150 fish per aquarium. The results showed that higher in growth body weight was obtained in immersion dose of 12 mg/L, by increment of about 37.4% higher compared to that of control. The immersion dose of 12 mg/L also increased specific growth rate by 29.3% higher (p<0.05) compared to control. Furthermore, at that immersion treatment, feed conversion ratio of blood worm and commercial diet decreased by 33.7% and 25.6% compared to control, respectively. Protein (7.15±0.08%) and lipid (9.95±0.10%) retentions in 12 mg/L rElGH-treated fish were higher (p<0.05) than those of control (protein 6.17±0.07%; lipid 5.73±0.06%). Lower in protein content (12.73%), while higher content in lipid (8.35%) and crude carbohydrate (3.22%) were found in 12 mg/L rElGH-treated fish compared to those of control (13.24%, 5.90%, and 1.76%, respectively). Thus, in general, rElGH immersion dose of 12 mg/L could generate high performances of eel juvenile, and application of rElGH can be useful to increase aquaculture eel production.Keywords: immersion dose, growth hormone, growth, glass eel

Growth and survival of giant gourami juvenile immersed in freshwater containing growth hormone Ratnawati, Pustika; Alimuddin, .; Arfah, Harton; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Growth of giant gourami is relatively slow. Administration of recombinant growth hormone (rGH) by severaltimes immersion in water containing 0.9% NaCl for one hour can significantly increase the growth. In thisstudy, giant gourami juvenile were immersed in freshwater containing 120 mg/L rGH to determine the longimmersion time that provides higher growth and survival. Juvenile at seventh-day-old after egg yolk absorbedwas immersed for 0.5, 1.0, 2.0, and 3.0 hours in rGH solution. As controls, fish was immersed in freshwaterwithout rGH (control 1: C1), and water containing 0.9% NaCl, and 120 mg/L rGH (control 2: C2). Fish waskept under controlled conditions in 20 L aquarium for three weeks, then in 50 L aquarium until at end ofexperiment, and fed at satiation. The results showed that highest growth in body weight, survival, and biomass(p<0.05) were obtained in rGH immersion treatment for 0.5 hour. Biomass of fish immersed by rGH for 0.5hour was about 32% higher compared to control C1, and 13% compared to C2. Survival of the 0.5 hour rGHimmersedfish (100%) was similar to control C1 and C2. Thus, immersion of giant gourami juvenile infreshwater containing rGH for 0.5 hour allowed highest growth rate, and this method is relatively easy to beadopted by fish hatchery.Keywords: immersion, hormone, growth, freshwater, giant gourami

Testicular cells transplantation of neon tetra Paracheirodon innesi in common carp Cyprinus carpio Firdaus, Muhammad; Alimuddin, .; Sumantadinata, Komar
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The neon tetra Paracheirodon innesi is one of the most popular ornamental fishes in the world. Its productivity seemed low because of the relatively low fecundity and hard to maintain the spawning condition. Transplantation of neon tetra testicular cells to common carp as surrogate broodstock, is a potential breakthrough for mass production of juvenile. We developed testicular cell transplantation by optimizing the size of donor that have highest spermatogonia proportion and testicular cell colonization level. The neon tetras divided into three groups of size based on the total body length (TL), namely: small (TL<18.00 mm), medium (TL=18.00–23.00 mm), and large (TL>23.00 mm). Then, we defined histomorphological characteristic of neon tetra testicular cells including diameter and volume of cell. Gonado somatic index (GSI) and proportion of spermatogonia from each group were also measured to determine group with highest spermatogonia proportion. Neon tetra testicular cells from each group that contain spermatogonial cells, were then injected into common carp larvae to examine it’s colonization level. The results of testicular cells histomorphological characteristic analysis showed that spermatogonia mean diameter and cell volume were 6.75±1.30 µm and 1,420.50±768.20 µm3, respectively. Medium-size fish group showed the highest spermatogonia proportion (20.95±1.29%) and also testicular cells colonization level (85.00±7.07%). In conclusion, as donor for testicular cells transplantation, medium-size neon tetra was better than large and small size. Keywords: testicular cells, transplantation, colonization, common carp, neon tetra ABSTRAK Ikan neon tetra Paracheirodon innesi merupakan salah satu ikan hias paling populer di dunia. Produktivitas ikan ini rendah karena memiliki fekunditas yang relatif sedikit dan kondisi pemijahan yang sulit dipenuhi. Transplantasi sel testikular ikan neon tetra kepada ikan mas sebagai induk semang merupakan terobosan potensial untuk produksi benih secara massal. Pada penelitian ini, transplantasi sel testikular dikembangkan melalui optimasi ukuran donor yang memiliki proporsi spermatogonia dan kolonisasi sel testikular tertinggi. Ikan neon tetra dibagi ke dalam tiga kelompok berdasarkan panjang total badan (PT), yaitu small (PT<18,00 mm), medium (PT=18,00–23,00 mm), dan large (PT>23,00 mm). Selanjutnya, ditentukan karakteristik histomorfologi dari sel testikular ikan neon tetra yang meliputi diameter dan volume sel. Indeks kematangan gonad (IKG) dan proporsi spermatogonia dari setiap kelompok juga diukur untuk menentukan kelompok dengan proporsi spermatogonia tertinggi. Sel testikular ikan neon tetra dari setiap kelompok ukuran yang mengandung sel spermatogonia selanjutnya diinjeksikan kepada larva ikan mas untuk mengevaluasi tingkat kolonisasinya. Hasil dari analisis karakteristik histomorfologi sel testikular menunjukkan bahwa rerata diameter dan rerata volume sel spermatogonia berturut–turut adalah 6,75±1,30 µm dan 1.420,50±768,20 µm3. Kelompok ikan ukuran medium memperlihatkan proporsi spermatogonia (20,95±1,29%) dan sekaligus tingkat kolonisasi sel testikular (85,00±7,07%) tertinggi. Dapat disimpulkan bahwa sebagai donor untuk transplantasi sel testikular, kelompok ikan neon tetra ukuran medium lebih baik dibandingkan dengan ukuran large dan small. Kata kunci: sel testikular, transplantasi, kolonisasi, ikan mas, ikan neon tetra

Effectivity of thyroxine and recombinant growth hormone on the growth of Siam-catfish larvae Sudrajat, Agus Oman; Muttaqin, Muhammad; Alimuddin, .
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Catfish hatchery requires technology and engineering to maximize the development and growth of fish. In this research the hormone thyroxine (T4), recombinant growth hormone (G) and mix of thyroxine and growth hormones (GT) were administered by immersion to enhance the development and growth of stripped catfish larvae. Research was using completely randomized design, with four treatments and five replications; A, control; B, thyroxine hormone (T4) 0.1 mg/L; C, T4 and G (GT) (0.1 mg/L and 10 mg/L); and D, G 10 mg/L. Immersion was performed for one hour. Results showed that the rate of yolk absorption at 16 hours post immersion was higher (P<0.05) in treatment B (96.98%) compared with treatments A (18.54%), C (20.59%), and D (32.90%). Larval growth of treatments B (24.85 mm) and C (24.00 mm) was higher (P<0.05) compared with treatments A (21.71 mm) and D (23.18 mm). Survival among treatments were similar (P>0.05). The size of liver cell and cytoplasm of treated larvae were larger than the control. Behavior of fish in the treatments B and C showed more active than the treatments A and D. Thus, combination of thyroxine and recombinant growth hormone treatment (C) has an efficient of yolk utilization, and higher in development and growth of stripped catfish larvae. Keywords: thyroxine, growth hormone, yolk absorption, growth, stripped catfish ABSTRAK Pembenihan ikan patin membutuhkan teknologi dan rekayasa untuk memaksimumkan perkembangan, dan pertumbuhan ikan. Pada penelitian ini dilakukan pemberian hormon tiroksin (T4), hormon pertumbuhan rekombinan (G) serta hormon gabungan antara hormon tiroksin dan hormon pertumbuhan (GT) melalui perendaman untuk memacu perkembangan dan pertumbuhan larva ikan patin siam. Penelitian menggunakan rancangan acak lengkap, dengan empat perlakuan dan lima kali ulangan; A, kontrol; B, hormon tiroksin (T4) 0,1 mg/L; C, T4 dan G (GT) (0,1 mg/L dan 10 mg/L); dan D, G 10 mg/L. Perendaman dilakukan selama satu jam. Hasil penelitian menunjukkan bahwa laju penyerapan kuning telur pada jam ke-16 setelah perendaman lebih cepat (P<0,05) pada perlakuan B (96,98%) dibandingkan dengan perlakuan A (18,54%), C (20,59%), dan D (32,90%). Pertumbuhan larva yang diberi perlakuan B (24,85 mm) dan C (24,00 mm) lebih tinggi (P<0,05) dibandingkan dengan perlakuan A (21,71 mm) dan D (23,18 mm). Tingkat kelangsungan hidup antarperlakuan tidak berbeda (P>0,05). Ukuran sel dan sitoplasma hati ikan perlakuan relatif lebih besar dibandingkan kontrol. Tingkah laku ikan pada perlakuan B dan C lebih aktif dibandingkan perlakuan A dan D. Dengan demikian kombinasi hormon tiroksin dan hormon pertumbuhan rekombinan secara bersama (C) memiliki efisiensi pemanfaatan kuning telur, perkembangan, dan pertumbuhan lebih tinggi pada larva ikan patin. Kata kunci: tiroksin, hormon pertumbuhan, penyerapan kuning telur, pertumbuhan, ikan patin siam

Artemia sp. as a DNA vaccine vector for common carp Cyprinus carpio larvae Nuryati, Sri; Hadiwibowo, Sekar Sulistyaning; Alimuddin, .
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Koi herpes virus (KHV) is one of the most common impetuses for disease on common carp Cyprinus carpio. Generally, viral disease is difficult to cure because virus is intra-cellular parasite, that virus survives, multiplies, and lives only if it on the host cell. Oral vaccine delivery through Artemia sp. is of one alternative way to overcome this problem. This experiment was carried out by analysis DNA vaccine expression encoding of glycoprotein gene (GP-11) on C. carpio. Bacteria containing plasmid Krt-GP-11 as vaccine is served through Artemia sp. as a vector. Artemia sp. was given for one and two times a week to three weeks old common carp. Organs of fish fed by Artemia sp. were analyzed every three days after vaccination. The expression of GP-11 in kidney in each treatment is also observed by the use of RT-PCR method, within ten days after vaccination. The experiment showed that dose of DNA vaccine in whole bacteria could be expressed is 106 cfu/mL in a once or twice provisions a week. DNA vaccine could be detected in three organs. RT-PCR analysis also showed that the expression of GP-11 can be detected in all tested organs. In conclusion, Artemia sp. can be used as a vector to carry plasmid GP-11 vaccine for common carp Cyprinus carpio larvae. Keywords: DNA vaccine, KHV, Artemia sp., common carp ABSTRAK Salah satu penyakit pada ikan mas (Cyprinus carpio) yang disebabkan oleh virus adalah koi herpes virus (KHV). Penyakit yang disebabkan oleh virus umumnya sulit untuk disembuhkan karena virus merupakan parasit intraseluler, yaitu virus hanya dapat hidup, bertahan hidup, dan memperbanyak diri di dalam sel inang. Metode pemberian vaksin DNA secara oral melalui Artemia sp. merupakan salah satu alternatif pengobatan yang diharapkan dapat menangani permasalahan penyakit pada ikan yang disebabkan oleh virus. Pada penelitian ini dilakukan uji ekspresi vaksin DNA yang menyandikan glikoprotein 11 (GP-11) pada ikan mas. Bakteri yang mengandung plasmid Krt-GP-11 sebagai vaksin diberikan melalui Artemia sp. sebagai pembawa vaksin. Pemberian Artemia sp. dilakukan satu dan dua kali seminggu pada ikan mas umur tiga minggu. Keberadaan DNA vaksin di usus, ginjal, dan insang dianalisis menggunakan metode PCR. Organ diambil setiap tiga hari setelah pemberian vaksin. Ekspresi gen GP-11 juga diamati pada organ ginjal di setiap perlakuan dengan menggunakan metode RT-PCR, pada sepuluh hari setelah pemberian vaksin. Hasil penelitian menunjukkan bahwa DNA vaksin yang diberikan dengan dosis 106 cfu/mL pada perlakuan satu dan dua kali seminggu dapat terdeteksi pada ketiga organ. Hasil RT-PCR menunjukkan bahwa ekspresi GP-11 dapat terdeteksi pada semua organ uji di setiap perlakuan. Dengan demikian Artemia sp. dapat digunakan sebagai vektor pembawa vaksin plasmid GP-11 dengan frekuensi pemberian vaksin untuk larva ikan mas. Kata kunci: vaksin DNA, KHV, Artemia sp., ikan mas

Karakterisasi cairan fermentasi daun mangrove Avicennia marina dan daya hambatnya terhadap bakteri penyebab penyakit ice-ice Samsu Adi Rahman; Sukenda; Widanarni; Alimuddin; Julie Ekasari
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRAK Cairan fermentasi daun mangrove Avicennia marina mengandung mikroorganisme, nutrient, dan metabolit sekunder. Penelitian ini bertujuan untuk mengidentifikasi bakteri dan senyawa dalam cairan fermentasi daun mangrove A. marina dan mengukur daya hambatnya terhadap bakteri patogen Stenotrophomonas maltophilia penyebab penyakit ice-ice pada rumput laut. Hasil analisis molekuler dengan target gen 16S rRNA menunjukkan bahwa bakteri dalam cairan fermentasi terdiri atas delapan jenis Bacillus, yaitu Bacillus subtilis MSAR-01, Bacillus megaterium MSAR-02, Bacillus firmus MSAR-03, Bacillus thuringiensis MSAR-04, Bacillus subterranerus MSAR-05, Bacillus vietnamensis MSAR-06, Bacillus sp. MSAR-07, Bacillus circulans MSAR-08, dengan daya hambat terbaik ditunjukkan oleh B. subtilis MSAR-01, B. vietnamensis MSAR-06, dan Bacillus sp. MSAR-07. Pemberian asam laktat, bakteriosin, cairan fermentasi total, dan supernatan sebanyak 15 mL menghasilkan daya hambat terhadap bakteri S. maltophilia lebih baik daripada menggunakan salah satu atau kombinasi beberapa jenis bakteri isolat. Daya hambat cairan fermentasi dan supernatan yang diperkaya bakteri tunggal lebih baik daripada pengayaan kombinasi bakteri. Kata kunci: Avicennia marina, fermentasi, ice-ice, mangrove

Identification of sex linked molecular markers in Indonesian giant freshwater prawn Macrobrachium rosenbergii Megawati, Novi; Alimuddin; Aliah, Ratu Siti
Jurnal Akuakultur Indonesia Vol. 20 No. 1 (2021): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.20.1.93-100

Male giant freshwater prawn grows faster than its female. Therefore, male mono sex culture is one of the solutions to improve aquaculture production. The all-male population of giant freshwater prawns can be produced by mating the neo-females (sex-reversed males) with the normal males. This study was aimed to identify the molecular markers related to the giant freshwater prawn sex. Specific primers were designed based on female-specific AFLP marker sequences to distinguish male and female sex on the prawns. Three locations for obtaining the Indonesian prawns in this study were Aceh, Sukabumi, and Solo. Based on the PCR analysis with MrMKn primers, 30 samples of female prawns had 100 % occurred DNA bands, while no DNA bands were obtained in all-male prawns from Solo. Nevertheless, MrMKn primers still detected 10–16 % male prawns from Sukabumi and Aceh. This indicated that MrMKn primers could not yet distinguish the male prawns for all populations. Moreover, the results suggested that the three prawn samples were different based on female-specific gene sequence. The MrMKn primers have the opportunity to be used in the selection of the female ZZ (neo-female) prawns from Solo without progeny test, so that the determination of female ZZ candidates can be identified more quickly. However, the primer still needs to be redesigned to distinguish neo-female prawns from Sukabumi and Aceh. Keyword: giant freshwater prawn, mono sex, neo-female, sex markers ABSTRAK Udang galah jantan lebih cepat tumbuh dibandingkan dengan betinanya sehingga budidaya udang galah monoseks jantan menjadi salah satu solusi untuk meningkatkan produksi budidaya. Populasi monoseks jantan udang galah dapat dihasilkan dengan mengawinkan neofemales (sex-reversed males) dengan jantan normal. Sistem kromosom pada udang galah berbeda dengan ikan. Individu betina bersifat heterogametik (WZ) dan jantan homogametik (ZZ). Dalam perkembangannya, terdapat kendala dalam menentukan individu neofemale yang memiliki kromosom ZZ. Berdasarkan pendekatan sistem kromosom tersebut, maka dapat dijadikan acuan untuk membuat marka molekuler terkait kelamin udang galah. Penelitian ini bertujuan mengidentifikasi marka molekuler terkait jenis kelamin pada udang galah. Primer spesifik didesain berdasarkan sekuen female specific AFLP marker untuk membedakan kelamin jantan dan betina pada udang galah. Tiga sumber udang galah digunakan dalam penelitian ini, yaitu Aceh, Sukabumi, dan Solo. Berdasarkan hasil analisis PCR dengan primer MrKNn, dari 30 sampel pada kelompok udang galah betina diperoleh hasil 100% pita DNA muncul, dan tidak terdapat pita DNA pada semua udang galah jantan asal Solo. Namun demikian, primer MrMKn tersebut masih mendeteksi sebesar 10–16% pada udang galah asal Sukabumi dan Aceh. Hal ini menunjukkan bahwa primer MrMKn belum dapat membedakan udang galah jantan dari semua populasi. Selain itu, dapat dikatakan bahwa ketiga udang galah uji adalah berbeda, khususnya sekuen gen spesifik betina. Primer MrMKn berpeluang digunakan dalam proses seleksi udang galah betina ZZ (neofemale) asal Solo tanpa harus melalui uji progeni sehingga penentuan kandidat betina ZZ lebih cepat teridentifikasi. Akan tetapi, primer masih perlu didesain ulang untuk membedakan neofemale asal Sukabumi dan Aceh. Kata kunci: marka kelamin, monoseks, neo-female, udang galah

Co-Authors . Irmawati . Kurdianto . Suharsono A. Octavera A. Santika Abinawanto ACHIS M. SIREGAR Achmad Sudradjat Ade Sunarma ADITYA BRAHMANDITO Afif Abdurrahman Afpriyaningrum MD AGUS O. SUDRAJAT Agus Oman Sudrajat Agus Priyadi Agus Priyadi Agus Priyadi Ahmad Muzaki AKHMAD TAUFIQ MUKTI Amrullah Amrullah Anang Hari Kristanto Andi Aliah Hidayani Andi Aliah Hidayani Andi Aliah Hidayani Andi Haerul Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi Tenriulo Andi Tenriulo Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANNA OCTAVERA Anny Hary Ayu Aras Syazili Arief Boediono Arief Boediono Arief Eko Prasetiyo Aris Tri Wahyudi Asep Permana Asep Permana Asep Sopian Asep Sopian ASMI CITRA MALINA A. R. TASSAKKA Aulia Saputra Aulia Saputra Ayi Santika Ayi Santika Ayi Santika Azis Azis Bagus Rahmat Basuki Bambang Iswanto Beginer Subhan Boyun Handoyo Boyun Handoyo Budimawan DARMAWAN SETIA BUDI Darussalam DEDI JUSADI DENNY KHAIRUDI Deny Sapto Chondro Utomo Desy Nurul Astuti Dewi Yuniati Dian Hardiantho Dian Hardiantho Dian Novita Sari Dian R Herdianto Dinamella Wahjuningrum Dinar Tri Soelistiyowati Dinar Tri Soelistyowati Dinar Tri Sulistyowati Dinar Tri Sulistyowati Dody Dharmawan Trijuno Dody Dharmawan Trijuno Dondy Arafat Dwi Hany Yanti Dwi Hany Yanti Dwi Yanti Emma Suryati Emma Suryati Enang Harris Enang Harris Enang Harris Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eny Heriyati ENY SUPARYANI Erma Primanita Hayuningtyas Erma Primanita Hayuningtyas Estu Nugroho Evi Tahapari F.H. Pasaribu Fadhila Maharani Putri FADHILLAH RAHMAWATI Fajar Anggraeni Fajar Maulana Fauzan Wahib Alsani Fitriyah Husnul Khotimah Fredinan Yulianda G. Yoshizaki GEMI TRIASTUTIK Gloria Ika Satriani Goro Yoshizaki GORO YOSHIZAKI Goro Yoshizaki Goro Yoshizaki Gunarto Gunarto Gusrina Gusrina H. Arfah Hafidah, Riva Hardiantho D Harton Arfah Hary Krettiawan Haryanti Haryanti HASAN NASRULLAH Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah HAWIS H. MADDUPPA Herjayanto, Muh. Huria Marnis Huria Marnis I.W. Teguh Wibawan Iis Diatin Ika Safitri Ikhsan Khasani Imron Imron Imron Imron INCE AYU KHAIRANA KADRIAH Indah Mastuti Indah Permata Sari Indra Lesmana Intan Febrianti Irin Iriana Kusmini Irma Andriani Irma Andriani IRMA ANDRIYANI Irmawati IRMAWATI IRMAWATI Irmawati, Budimawan Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal IRVAN FAIZAL Irzal Effendi Ita Djuwita Ita Djuwita Jasmadi Jasmadi Jean-Christophe Avarre Joko Santoso Joni Haryadi Jr., Muhammad Zairin Julie Ekasari K. Chaitanya Deepti K. Sumantadinata Kamaruddin Ketut Mahardika Ketut Sugama khairuzzazi KOMAR SUMANTADINATA Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Nirmala Kurnia Anggraini Rahmi Lies Emmawati Hadie Luky Adrianto M. SASMITO JATI M. Zairin Junior M.Y. Jamal Maharani, Adinda Maskur Maskur Megawati, Novi MEIMULYA MEIMULYA Melta Rini Fahmi Mia Setiawati Mira Mawardi Mochamad Syaifudin Moh. Tauhid Umar Mohammad Zairin Mohammad Zairin Mubinun Mubinun Muh Alias L. Rajamuddin Muh. Alias L. Rajamuddin MUHAMMAD AGUS SUPRAYUDI Muhammad Firdaus Muhammad Hunaina Fariduddin Ath-thar Muhammad Hunaina Fariduddin Ath-thar Muhammad Husni Amarullah Muhammad Muhammad Muhammad Muttaqin Muhammad Safir Muhammad Yamin Muhammad Zairin Jr Muhammad Zairin Jr Muhammad Zairin Jr. MUHAMMAD ZAIRIN JR. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mulyasari Mulyasari MUNTI YUHANA Muslim Muslim Muzahar N.A. Maswan Nadia Ayuningthias NADIARTI NADIARTI NITA RUKMINASARI Nita Rukminasari Nita Rukminasari Novi Megawati Novi Megawati Nunak Nafiqoh Nunuk Listiyowati Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo NURBETY TARIGAN Nurly Faridah Nurly Faridah O. Carman O.Z. Arifin Odang Carman P. Ratna Kumar Petrus Rani Pong-Masak Priadi Setyawan Pustika Ratnawati Putriku Nadzira Sativa R Rifai R.D. Soejoedono Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi Ratu Siti Aliah Ratu Siti Aliah RATU SITI ALIAH Ratu Siti Aliah Rendy Ginanjar Reni Agustina Lubis Ridwan RIDWAN AFFANDI Rommy Suprapto Ruby Vidia Kusumah Rudhy Gustiano RUSTIDJA S. Mariam Samsu Adi Rahman Sari Budi Moria Sembiring Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Setiawati M Shabarullah Siska Aliyas Sandra Siti Aslamyah Siti Fadilah Siti Khodijah Siti Subaidah Siti Zuhriyyah Musthofa Sitti Fakhriyyah SLAMET SUBYAKTO Soelistyowati DT Soko Nuswantoro Sri Murtini Sri Nuryati Sri Nuryati St. Hidayah Triana Suci Antoro Sukenda, Sukenda, SUKMARAHARJA A. TARIGAN Sularto Sularto Sularto Sularto Sutanti Sutanti Syuhada Restu Danupratama T. Takeuchi Tatag Budiardi Toshio Takeuchi Tristiana Yuniarti Uswatun Hasanah Uswatun Hasanah Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Wartono Hadie Wartono Hadie Wartono Hadie Wartono Hadie Wartono Hadie Wedaraningtyas Nugrahani Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wiwien Mukti Andriyani Wiwien Mukti Andriyani Y. Hadiroseyani Yanti Inneke Nababan yogi Himawan Yulintine Yulintine Yuni Puji Hastuti Yushinta Fujaya Yushinta Fujaya Zairin Jr M Zakki Zainun

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