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All Journal EJIP BIOL Media Eksakta Jurnal Penelitian dan Pengembangan Pendidikan Jurnal Kreatif Tadulako Online Biocelebes Biology, Medicine, & Natural Product Chemistry EduChemia: Jurnal Kimia dan Pendidikan EDUKASI Jurnal Penelitian Pendidikan IPA (JPPIPA) INKUIRI : Jurnal Pendidikan IPA Jurnal Review Pendidikan Dasar : Jurnal Kajian Pendidikan dan Hasil Penelitian Bubungan Tinggi: Jurnal Pengabdian Masyarakat Jurnal Riset Pendidikan MIPA Koordinat : Jurnal Pembelajaran Matematika dan Sains Bioscientist : Jurnal Ilmiah Biologi EduLine: Journal of Education and Learning Innovation Bioed : Jurnal Pendidikan Biologi PTK: Jurnal Tindakan Kelas Edukasia: Jurnal Pendidikan dan Pembelajaran Jurnal Ilmiah Profesi Guru (JIPG) Edu Research : Jurnal Penelitian Pendidikan Jurnal Kreatif Online Jurnal Indonesia Sosial Teknologi DIROSAT: Journal of Education, Social Sciences & Humanities Indonesian Journal of Education Research (IJoER) Equator Science Journal (ESJ) Mitra Sains Jurnal Akademika Kimia Jurnal Pendidikan MIPA Journal of Biology Science and Education
Mohammad Jamhari
Program Studi Pendidikan Biologi, Fakultas Keguruan Dan Ilmu Pendidikan, Universitas Tadulako, Palu, Sulawesi Tengah, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Identification of Salmonella spp. Contamination in Gado-Gado Sold Around the Tadulako University Campus Pebi Angreni; I Nengah Kundera; Yulia Windarsih; Mohammad Jamhari; Vita Indri Febriani; Abdul Ashari
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.787-793

Abstract

Salmonella spp. are Gram-negative rod-shaped bacteria commonly found in the gastrointestinal tract of humans and animals and are recognized as one of the major causes of foodborne diseases. This study aimed to identify the presence of Salmonella spp. in gado-gado sold in the canteens of Tadulako University. This research employed a descriptive exploratory laboratory design. Samples were collected from five different canteens using a random sampling technique. Microbiological analyses were conducted through presumptive and confirmatory tests using the Most Probable Number (MPN) method, Total Plate Count (TPC), Gram staining, cultivation on selective Salmonella–Shigella Agar (SSA), and biochemical testing using Triple Sugar Iron Agar (TSIA). The MPN results showed an average value of 4,900 MPN/mL of sample, while the TPC reached 35,800/ml sample. These values indicate that the food product does not meet the microbiological safety standards established by the Indonesian Ministry of Health, as the TPC exceeds the maximum permissible limit of 104 CFU/mL.
Identification of Escherichia coli in Ice Dawet Sold in the Palu City Area Yosphita Sevianti; I Nengah Kundera; Musdalifah Nurdin; Yulia Windarsih; Abdul Ashari; Mohammad Jamhari
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.795-801

Abstract

Escherichia coli is a Gram-negative bacterium from the family Enterobacteriaceae found in the human body and is used as an indicator of hygiene in water, food, or beverages. The purpose of this study was to identify the presence of E. coli in ice dawet sold in the Palu City area. This study used a descriptive-exploratory design with the Most Probable Number (MPN) method, Total Plate Count (TPC), and biochemical testing using Triple Sugar Iron Agar (TSIA) medium. Samples were collected from five locations in the Palu City area using simple random sampling. Based on the results, the Most Probable Number (MPN) test produced an average value of 18.700 MPN/mL of sample, while the Total Plate Count (TPC) test showed an average value of 26.800 CFU/mL of sample, indicating that the samples did not meet the standards set by the Indonesian National Standard (SNI) under the Ministry of Health, which states that the maximum allowable microbial contamination in beverages is 0/100 mL. Confirmatory tests on Eosin Methylene Blue Agar showed colony growth with a metallic green sheen and reddish coloration, characteristic of Gram-negative bacilli. The results of the Triple Sugar Iron Agar biochemical test were positive, indicating biochemical characteristics consistent with E. coli. Therefore, the findings confirm the presence of E. coli in the analyzed samples.
Species and Cladystic of Butterflies (Lepidoptera) in Malonas Village, Dampelas District, Central Sulawesi Devi Devi; Mohammad Jamhari; Manap Trianto; Yulia Windarsih; Rafiqa Rafiqa; Fatmah Dhafir
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.763-769

Abstract

Butterflies are a group of insects belonging to the order Lepidoptera, commonly known as scale-winged insects. This study examines the kinship relationships of butterfly species (Lepidoptera) based on morphological and morphometric characters in Malonas Village, Dampelas District, Central Sulawesi, an area that has not been previously documented. The objectives of this study were to identify butterfly characters through morphological and morphometric analyses and to construct kinship patterns that reflect the level of phylogenetic similarity among butterfly species. The research was conducted using a descriptive exploratory method. Sampling was carried out across all areas of Malonas Village, including residential areas, rivers, forests, and plantations, using a roaming survey method with free collection techniques, resulting in 10 butterfly species with a total of 30 individuals. Morphological and morphometric analyses included measurements of wing dimensions and major body parts. Data were analyzed using Principal Component Analysis (PCA) and the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) to determine kinship patterns. The UPGMA analysis showed that all species had a relatively high level of similarity, with a similarity value of approximately 0.78, indicating close relationships among species. PCA results revealed that two principal components (PC1 and PC2) explained 67.0% of the total morphological variation, accounting for 45.3% and 21.7%, respectively. Loading plots indicated that morphometric characters related to wing size and proportions were the main contributors to the formation of the principal components. Biplot graphs demonstrated species grouping based on similarities in morphological and morphometric characters, as well as positive correlations among variables within the same quadrant. The results of this study confirm that wing morphometric characters play an important role in explaining morphological variation and phylogenetic relationships of butterflies in Malonas Village.
Co-Authors A.A. Ketut Agung Cahyawan W Abd Hakim Laenggeng, Abd Hakim Abd Rauf Abd. Rauf Abdul Ashari Abdul Rahman Abdul Rahman Achmad Ramadhan Achmad Ramadhan Adianingsih Adianingsih Adianingsih, Adianingsih Afadil Agus Rahmatullah Aidiyanda, siskha Ainun Sampede Aisyah Alivinda Aulia Safira Musyarofah Amalia Buntu Amiruddin Kade Amiruddin Kasim Amram Rede, Amram Amran Rede Amran Rede Andi Tanra Tellu Angelia, Helena Ani Sintiawati Ardiansyah Arham Arham, Arham Ashari, Abdul Asmara, Dudun Candra Asmawir Astija Astija Aulia, Azni Ayu - Lestari, Ayu - Aziza, Nurul Inaya Azza Liarista Anggraini Azzahra, Fitri Azzajjad, Muhammad Fath Bialangi, Mursito S. Binti Non Shamdas, Gamar Budiarsa , I Made Bustamin Bustamin Bustamin Bustamin Bustamin Datubaringan, Jein Daud Karel Walanda Devi Devi Dewi Purwasi Samaela Dewi Satria Ahmar Dewi Tureni Eka Dewi Erica Meilia Safitri Fadila, Nurul Faisah, NUrul Fajri, Ruri Rizkia Fatmah Dhafir Fatrah, Fatrah Febriawan, Aan Fhauzan, Fuad Finta Amalinda Fitri Azzahra Gaffar, Musdalifa Gamar Binti Non Shamdas Grasela, Nadia Hadi Pramono Haeruddin Haeruddin Haeruddin Haeruddin Haeruddin Haeruddin Haeruddin Haerudin Haerudin Handayani, Estin Nursanti Handayani, Rif’ati Dina Hasan, Isharyadi Hayyatun Mawaddah Hiska, Hiska Hj. Musdalifah Nurdin Humaira humaira I Made Budiarsa I Nengah Kundera Irda Irda Irma Wati Irsan Irsan Istikomah Jamaludin M Sakung Jumalia Jumalia Kairupan, Youla Fristin Kusumaningrat Laenggeng , Abd. Hakim Laksono Trisnantoro Lamuke, Siti Rosima I. Latifa, Latifa Lestari M.P Alibasyah Lestari M.P. Alibasyah Lilies Lilies Lilies Lilies Lilies Lilies Lilies Lilies Lilies N Tangge Lilies N Tangge Lismawati Sahib Louto, Fadlun Fadilawati Lusiana Lusiana M. Thahir, Faizah Manap Trianto Mardiana, Indhira Marungkil Pasaribu Masriani . Masyita Masyita Maulidiyah, Arum Mestawaty Mestawaty Moh Fadil Moh Tri Ramadhan Moh. Nasihin Muchlis L. Djirimu Muhammad Zaky Munifa, Siti Murniati . Mursito S. Bialangi Musdalifah Nurdin Muslimin Musripan Abd Muis Nadia Ni Gusti Ayu Galuh Candra Kirana Nur Fahmi Safrianti Nur Rahmah Nurfatia, Nurfatia Nuriyani, Emy Nurlira Mei Widhiyanti Nursetya, Kartini Palangi, Julmita Palewang, Yuliana Y. Pebi Angreni Putri Rachmawati, Putri Putri, Riska Ananta Rafia Rafiqa Rafiqa Rafiqa RAFIQA RAFIQA, RAFIQA Rafiqah Rafiqah, Rafiqah Rahmadani, Nurlaila Wulan Ramadani, Ersa Ramadhan, Moh Tri Ramadhanil Ramadhanil Ramayana Ratman Ratman Ratman Ratman Ratman Raya Agni Renold Renold Reskywanti, Reskywanti Resmin Djafar Rif’ati Dina Handayani Rif’ati Dina Handayani Rif’ati Dina Handayani Ritman Ishak Paudi Ritman Ishak Paudi Ritman Paudi Rizka Fardha Rukmana Rukmana Rukmana, Rukmana Safarni, Safarni Safitri, Erica Meilia Safrianti, Nur Fahmi Salim Mulyadi Koolang Samsurizal, M. Sulaeman Sarnan, Etry Melatry Shamdas, Gamar B.N Shamdas, Gamar B.N. Sintiawati, Ani Sri Wahyuni Sri Wahyuni Sudarti Sudarti Sudarti Sudarti Sudarti Sudarti Sulastriani Sunarti Sunarti Supriyatman, Supriyatman Susanna Susanna Syamsul Arifin Syarifah Hadijah Syech Zainal Tasriah, Tasriah Tiro, Frelyana Tiwow, Vanny M. Vita Indri Febriani Wanty Aprilianti Wati, Irma Widyastuti Umar Widyawati, Dyah Windarsih, Yulia Yandi ary Pratama Yosphita Sevianti Yuniarti Zazila, Siqna Al Zulaikhah Dwi Jayanti Zulham A. Ranya