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All Journal Media Kesehatan Masyarakat Indonesia Jurnal Natur Indonesia JURNAL SAINS DAN TEKNOLOGI INDONESIA Journal of Marine Research BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Forestry Research Jurnal AgroBiogen Buletin Iptek Tanaman Pangan EDUKASIA MEDISAINS Bioeksperimen: Jurnal Penelitian Biologi Forum Geografi Jurnal Florea JOURNAL ONLINE OF PHYSICS Jurnal Bioedukasi BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Penelitian Karet Jurnal Pendidikan Biologi SASINDO Science and Technology Indonesia Journal of Language and Literature Jurnal Geologi dan Sumberdaya Mineral (Journal of Geology and Mineral Resources) Jurnal Kesehatan Bakti Tunas Husada Metode : Jurnal Teknik Industri ANNALES BOGORIENSES Al-Madrasah: Jurnal Ilmiah Pendidikan Madrasah Ibtidaiyah Jurnal Medik Veteriner Media Keperawatan Indonesia Bioedusiana: Jurnal Pendidikan Biologi JURNAL Al-AZHAR INDONESIA SERI HUMANIORA Jurnal Riset Kesehatan Poltekkes Depkes Bandung International Journal for Educational and Vocational Studies Jurnal Kesehatan Karya Husada Jurnal Abdimas Madani dan Lestari (JAMALI) Jurnal Geofisika REiLA: Journal of Research and Innovation in Language Jurnal Metaedukasi : Jurnal Ilmiah Pendidikan Lahjah Arabiyah: Jurnal Bahasa Arab dan Pendidikan Bahasa Arab Bioeduca : Journal of Biology Education Journal of Earth and Marine Technology (JEMT) Euler : Jurnal Ilmiah Matematika, Sains dan Teknologi MAHESA : Malahayati Health Student Journal JoMMiT : Jurnal Multi Media dan IT Journal of Applied Sciences, Management and Engineering Technology (JASMET) Muhammadiyah Journal of Geriatric Indonesian Journal of Earth Sciences Journal of Applied Nursing and Health Jurnal Ilmiah Kesehatan (JIKA) Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan QULUBANA: Jurnal Manajemen Dakwah At-Tajdid: Jurnal Ilmu Tarbiyah HEUTAGOGIA: Journal of Islamic Education Tanfidziya: Journal of Arabic Education Makara Journal of Science Jurnal Manajemen, Organisasi, Dan Bisnis Jurnal Abdi Kesehatan dan Kedokteran Investama : Jurnal Ekonomi dan Bisnis Eduvest - Journal of Universal Studies Jurnal Pengabdian Pada Masyarakat Jurnal Teknologi Informasi Jurnal Keperawatan Innovative: Journal Of Social Science Research Sasindo : Jurnal Pendidikan Bahasa dan Sastra Indonesia Transforma: Jurnal Manajemen Journal Economic Management and Business Kapuas Al Itimad: Jurnal Dakwah dan Pengembangan Masyarakat Islam Jurnal Pengabdian Masyarakat Al-Wasatiyah: Journal of Religious Moderation Jurnal Agro Veteriner Jurnal Geosaintek Annales Bogorienses Al-I'timad: Jurnal Dakwah dan Pengembangan Masyarakat Islam
Suharsono Suharsono
Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian, PO Box 66 Malang Telp. (0341) 801468
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PlGF Levels and Blood Pressure in Pregnant Women with Hypertension Simanjuntak, Veronica; Runjati, Runjati; Suharsono, Suharsono; Arwani, Arwani
Jurnal Ilmiah Kesehatan (JIKA) Vol. 6 No. 1 (2024): Volume 6 Nomor 1 April 2024
Publisher : Sarana Ilmu Indonesia (salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v6i1.647

Abstract

Hypertension in pregnancy is one of the causes of morbidity and mortality in mothers and babies. PlGF has been shown to be associated with the diagnosis of hypertensive disorders in pregnancy. Pregnant women with hypertension are characterized by reduced circulating PlGF levels, even before clinical signs and symptoms. A decrease in the amount of PlGF is positively correlated with the severity of disease progression This study aimed to analyze the relationship between PlGF levels and blood pressure in pregnant women with hypertension. Observational analytic with cross sectional study design.  Sampling was total sampling. The number of subject were 34 peoples. The results showed that there was no significant correlation between PlGF levels and blood pressure in pregnant women with hypertension. PlGF levels have no relationship with blood pressure.
Effectiveness of Labor Dance on Duration of the First Active Phase of Labor in Nulliparous Women Meta, Raharni Rosfiyanti; Sumarni, Sri; Suharsono, Suharsono
Jurnal Ilmiah Kesehatan (JIKA) Vol. 6 No. 2 (2024): Volume 6 Nomor 2 Agustus 2024
Publisher : Sarana Ilmu Indonesia (salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v6i2.724

Abstract

Prolonged labor, also known as failure to progress, occurs when the total duration of labor is more than 24 hours and if not handled properly will cause various complications for the mother and baby and even lead to death. This study aimed to determine the effectiveness of labor dance on the duration of the first active phase of labor in nulliparous women. This study was a true experimental randomized controlled trial (single blind) with a posttest only with control group design. This research was conducted from 1 February to 31 March 2023 at the Central General Hospital Prof. dr.I.G.N.G Ngoerah Denpasar and Regional General Hospital Wangaya Denpasar. 42 primigravida mothers were randomly selected according to arrival, with 21 people in the intervention and control groups. Data were analyzed using the Mann Whitney test. The average length of the first stage of labor in the experimental group was 252,62 minutes and in the control group was 355,95 minutes (p-value=0,000); effect size of 1,61 (strong effect) with a percentage of 94,5%. There was a statistically significant difference in the duration of the active phase I stage of labor between the experimental and control groups. It is hoped that health workers, especially midwives, will
ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE SUHARSONO, SUHARSONO; TRISNANINGRUM, NIKEN; SULISTYANINGSIH, LULUT DWI; WIDYASTUTI, UTUT
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.362 KB) | DOI: 10.11598/btb.2009.16.1.64

Abstract

Metallothionein is an important protein for detoxifying heavy metal ions. This research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affine. Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. The MaMt2 cDNA was successfully isolated by PCR technique. The MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5α. DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. This cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T easy. The deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs.   Key words: cDNA, metallothionein, Melastoma affine, cloning, cysteine
Partial Purification, Characterization, and Application of Extracellular Aspartic Protease from Lactobacillus casei WSP in Producing the Bioactive Peptides with Antibacterial and Antioxidant Activity Solikhin, Akhmad; Mustopa, Apon Zaenal; Suharsono, Suharsono; Putranto, Wendry Setiyadi
Annales Bogorienses Vol. 22 No. 2 (2018): Annales Bogorienses
Publisher : BRIN

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Abstract

Lactobacillus casei WSP-derived an aspartic protease was sequentially purified by using chromatography gel filtration sephadex G-50. It resulted in a 22.81-fold increase of specific activity (51.5 U/mg) with a final yield of 1.9%. The estimated molecular weight of the purified enzyme was 37 kDa and showed gelatinolytic activity in zymogram assay. The enzyme exhibited optimum activity at 40ºC and pH 6 with casein as the substrate. Enzyme activity was significantly inhibited by pepstatin A (0.5 mM and 1 mM), confirming that this enzyme is a group of aspartic proteases, while other inhibitors such as EDTA, PMSF and iodoacetic acid showed no inhibition effect on the activity of enzyme. The addition of metal ion to the enzyme decreased enzyme activity, indicating the proteolytic enzyme was metal ion- dependent. Denaturant such as DDT tended to increase caseinolytic activity. Furthermore, this enzyme was capable of generating the new peptides from skimmed milk with the size 8 kDa, 10 kDa and 15 kDa. These peptides have potential as antibacterial and antioxidant agents.
Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Susanti, Idha; Suharsono, Suharsono; Widyastuti, Utut; Siregar, Ulfah Juniarti; Tjahjoleksono, Aris
Annales Bogorienses Vol. 21 No. 2 (2017): Annales Bogorienses
Publisher : BRIN

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Abstract

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media. This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment. Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”. All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos
Constitutive Expression of Candida antarctica Lipase B (CALB) in Pichia pastoris Using pGAPZα Vector Wahyuni, Febriana Dwi; Fuad, Asrul Muhamad; Suharsono, Suharsono
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
Publisher : BRIN

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Abstract

The synthetic gene of CalBsyn was previously constructed to encode Candida antarctica lipase B (CALB). Lipase of CalBsyn gene is slightly different from wild type CALB (CALB-wt) where it has three amino acids substitutions at different positions, i.e. V210I, A281E, and V221D, in order to improve its thermostability and catalytic efficiency. The CalBsyn gene was isolated from pJ912-CalBsyn vector by digestion using XhoI restriction enzyme. The 1136 bp fragment of CalBsyn gene was then ligated to pGAPZα expression vector and transformed into Escherichia coli TOP10F’ to obtain recombinant vector pGAPZα-CalBsyn. The result show that pGAPZα-CalBsyn recombinant vector was successfully transformed into E. coli TOP10F’ with transformation efficiency of 4.11x103 cfu/μg plasmid DNA. The pGAPZα-CalBsyn recombinant plasmid was successfully introduced into Pichia pastoris SMD1168H using electroporation method with transformation efficiency of 1.01x102 cfu/μg DNA. Recombinant protein expression was analyzed in several selected P. pastoris recombinant strains. Qualitative lipase activity assays results show that transformed P. pastoris-produced extracellular recombinant lipase (CALB) showing lipolytic activity; while results of quantitative lipase activity assays show that this Pichia-derived lipase achieved an activity of 6.35 Units/mL within 48 hours. SDS-PAGE analysis confirms the succesfull expression of CALB protein with molecular size was approximately 45 kDa.
Strategi Pengembangan Ekowisata Mangrove di Pesisir Pantai Kertomulyo, Kecamatan Trangkil, Kabupaten Pati Sirojuddin, Wildan; Suharsono, Suharsono; Alimuddin, Khasan
Al-I'timad: Jurnal Dakwah dan Pengembangan Masyarakat Islam Vol 2 No 1 (2024): April 2024
Publisher : Mafa Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35878/alitimad.v2i1.927

Abstract

This research aims to find out the strategy for developing Mangrove ecotourism in the coastal area of Kertomulyo village, Trangkil sub-district, Pati Regency. This research is classified as a type of field research, with a qualitative-descriptive method. The data collection techniques use observation, interviews and documentation. And data analysis using SWOT theory. The results of this research show that the strengths possessed by the organization in carrying out the strategy that has been determined are the availability of applicable laws and regulations, the availability of human resources both in quality and quantity, sufficient availability of supporting facilities and infrastructure, and awareness from the public. The weaknesses that an organization has in implementing the strategy that has been determined can be an obstacle to the success of the strategy, such as: Lack of professionalism of the employee apparatus. Public awareness regarding the implementation of development has not yet accumulated. Accurate data regarding the development that will be implemented is not yet available. There has not been a culture of transparency in budget management and good management. Opportunities or opportunities that an organization has in carrying out a predetermined strategic plan can be supporting factors that will have a positive impact on the success of the strategy, including: The existence of business opportunities. There is development in the business world. The occurrence is cross-sectoral. Increased mobility of goods and services. There is coordination from related agencies. Threats definitely occur in every organization which can be a trigger to increase an organization's efforts and creativity in carrying out planned strategies. Some of the threats in tourism strategies are: High business competition, lack of development of facilities and infrastructure, lack of government role, and lack of attractiveness and uniqueness of tourist attractions.
The Correlation between Self Efficacy with Problem Solving Ability of Student in Studying Ecosystem Rasyidina, Sumayya Ainur; Suharsono, Suharsono; Meylani, Vita
Bioeduca : Journal of Biology Education Vol. 5 No. 1 (2023): Bioeduca : Journal of Biology Education
Publisher : Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/bioeduca.v5i1.14524

Abstract

Students need the ability to explore, think critically and analyze a problem for a logical and appropriate solution. Problem solving abilities accompanied with a belief in their abilities (self efficacy) will maximize student abilities in and get maximum grades. This research aims to analyze the correlation between self efficacy and students' problem-solving abilities in ecosystem material. The method used is a quantitative method with a correlational approach (relationship study). The data collection technique was in the form of a problem-solving ability test in the form of an essay about ecosystem and a self efficacy questionnaire. The data analysis technique uses the Product Moment correlation test. Based on the results of data analysis and hypothesis testing, a correlation coefficient value of 0.675 was obtained, which means that the correlation coefficient level is strong. It can be concluded that there is a correlation between self efficacy and problem-solving abilities of students in ecosystem material with a strong correlation coefficient.
Agrobacterium-Mediated Genetic Transformation of Seaweed Kappaphycus alvarezii Using Gα Gene and Callus Cultures Sulistiani, Erina; Suharsono, Suharsono; Supena, Ence Darmo Jaya; Miftahudin, Miftahudin
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.176 KB) | DOI: 10.11598/btb.2019.26.1.989

Abstract

Cottonii seaweed (Kappaphycus alvarezii Doty) is one of the most important commercial sources of carrageenan, which is widely used in the pharmaceutical and food industries. A major problem in the cultivation of this seaweed is ice-ice disease, which is caused by extreme changes in environmental conditions such as temperature and seawater salinity. Gene transformation to produce transgenic Kappaphycus lines that are tolerant to environmental stress is a potential solution to this problem. The Gα gene, which encodes the heterotrimeric G-protein alpha subunit, plays an important role in tolerance to biotic and abiotic environmental stress. This study aimed to: (a) introduce the Gα gene into the callus cells of K. alvarezii and regenerate transformed callus cells into transgenic plantlets; and (b) determine the appropriate concentration of acetosyringone and Agrobacterium tumefaciens strain for successful gene transfer into the callus of K. alvarezii. The callus cells of K. alvarezii were transformed using Agrobacterium tumefaciens strains LBA4404 and EHA105 carrying the expression vector pGWB502-Gα under the control of the CaMV-35S promoter. The calli and A. tumefaciens were co-cultivated in different concentrations of acetosyringone (20, 40, and 60 mg/L). The regeneration of transformed callus cells into transgenic plantlets was successfully achieved using the somatic embryogenesis technique. The results showed that the highest percentage of putative transgenic micropropagule formation occurred at acetosyringone concentrations of 20–40 mg/L. Polymerase chain reaction (PCR) analysis of twenty regenerated plantlets indicated that the Gα gene was successfully introduced into the genomic DNA of all samples. The highest transformation efficiency was obtained from the 20–40 mg/L acetosyringone co-cultivation treatment (22–28%). The transformation efficiency produced by Agrobacterium tumefaciens EHA105 (23%) was not significantly different from that produced using strain LBA4404 (15%).
Co-Authors AA Sudharmawan, AA Adi Isworo, Adi Adikara, Tatang Santanu Agung Setiabudi Aini, Kuratul Akhmad Solikhin AKIHO YOKOTA Al arif, Mohammad Anam Al Firah, Al Firah ALEX HARTANA Alimuddin, Khasan Ana Quthratun Nada Angga Sugiarto Anik Herminingsih Apon Zaenal Mustopa Arafat, Yasir Arif Rianto Budi Nugroho Aris Tjahjoleksono Arwani Arwani Ashadi Ashar N, Novema Aspariansyah, Aspariansyah Asrul Muhamad Fuad, Asrul Muhamad Astanto Kasno Avellyn Shinthya Sari Aviadi, Dedi Badriah, Liah Bambang Sarwono Bambang Yulianto Budianingsih, Budianingsih Cahyadi, Tedy Agung Caswita Caswita Cici Indriani Dalimunthe damayanti, dini Dani Ramdani, Dani Dessy Adelin Destiana, Destiana Didy Sopandie Diki Muhamad Chaidir Diponegoro, Muhammad Djaka Marwasta Egi, Egi Nuryadin Eko Teguh Paripurno Elfa Cahya Jamiati Endang Surahman Erawati, Erna Fachry Abda El Rahman Faisal Rachman Febriana Dwi Wahyuni Feri, Zefki Okta Ferry Faisal, Ferry Firdaus, Syarifin Fitri Damayanti Fitri Damayanti Fitri Wibowo Fitri, Novi Aryani Gandhi Sutjahjo Gesa Cantya Primatara Gunawan, Heru Hadiarto, Toto Hamim Hamim Hapsari, Agustina Retno Harijani, Nenny Hariyadi, Rahmat Hasan Labiqul Aqil Hermana, Salma Junaina Hidanah, Sri Hutabarat, Madya I, Heriberta Reny Idayati, Tutik Rosita Idha Susanti Iif Rahmat Fauzi, Iif Rahmat Ika Barokah Suryaningsih Ika Mariska IMAM MUSTOFA Irbah, Ariq Nurjannah Ishaq, Rizal Maulana Jayawarsa, A.A. Ketut Kagoya, Tundani Kartawan, Kartawan Karuwal, Ritha Lusian KINYA AKASHI Komul, Marsha Krisdiana Wijayanti Kristanto, Paustinus Edi Kusuma, Amino V.A. Kusumayudha, Sari Bahagiarti Kuswanto, Edi Lulut Dwi Sulistyaningsih Lulut Handayani Maemonah, Maemonah Mahmudah, Uswatun marwoto marwoto Meta, Raharni Rosfiyanti Miftahudin . Mochamad Widjanarko Muhammad Helmi Muhammad Jusuf Muhammad Sirril Wafa Muin, Muhammad Rusli Munasik Munasik Mustafawi, Wike Z. Mustofa, Romy Faisal Mustopa, Apon Z. Mutiara, Frista Mycelia Paradise Nabiila, Alyaa Nainggolan, Adinda Rachel Nasrul, Herni Widiyah Ningrum, Hanis Rachna Noor Faidah, Noor Noorikhsan, Faisal Fadilla Novriyandi Hanif Nugroho, Arif Rianto Budi Nugroho, Arif Riyanto Budi Nur Izzati, Aisyah Nuriyatul Fitriya Nurjanah, Fitri Nuryadin, Egi Oktavianti Oktavianti Palaniappan, Ananda Kumar Pausta Yugianus Pertiwi, Nadira Rasya Polosoro, Aqwin Pramono, Sidik Prasetya, Wandy Murti Prasetyadi, C Prasetyadi, Carolus Prasetyadi, Corolus Pratama, Yudha Agung Pujiastuti, Rr Sri Endang purwoko, devit RADITE TISTAMA Rasyidina, Sumayya Ainur RATIH DEWI HASTUTI Rikoh Manogar Siringoringo Rinaldi Rizal Putra, Rinaldi Rizal Rivai, Muhamad Rizal Rochadi Abdulhadi Rohananingsih, Rohananingsih Rojabtiyah, Rojabtiyah Rondhianto Rondhianto S. Hidayat, S. Safari, Neng Ayu Mustika Sahputra, Ngatno Sahroni, Sahroni Sahupala, Sapiah Samuel Agus Triyanto, Samuel Agus Santika, Wulan Puja Saputra, Muhammad Wahyu Saputri, Niken Anggraini Sri Sari Bahagiarti Kusumayudha Sari Bahagiarti Kusumayudha Sari, Avellyn Shintya Sari, Fatihah Fajar Sarmanu, Sarmanu Sarsidi Sastrosumarjo Septiana, Erni Setiahadiwibowo, Ajimas Pascaning Shohifaha , Salsabila Sholihin, Achmad Simanjuntak, Veronica Sirait, Budi Chrismanto Sirojuddin, Wildan Siti Zulaeha, Siti sodiq, alwi mahardhika Soekarta, Rendra Solly Aryza Sri Hidanah Sri Langgeng Ratnasari SRI RAHAYU Sri Sumarni Sugiyarto, Angga Sulistiani, Erina Sulistyaningsih, Yohana C Sulistyowati, Dina Indrati Dyah Sunarko Sunarko Supena, Ence Darmo Jaya Supranianondo, Koesnoto Susanto, Abiyyu Naufal Sutarto Sutarto Sutrisno, Sutrisno Suyanta Suyanta Ta'adi, Ta'adi Teuku Tajuddin Tita Damayanti Lestari Titik Sudartinah Titin Handayani Trisnaningrum, Niken Trisnowati, Yuni Dwi Trustinah Trustinah Ulfah Juniarti Siregar Utut Widy Astuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Vita Meylani Wahyu Hidayat Wamiliana Wamiliana Wendry Setiyadi Putranto Wening Enggarini Widya Paramita Lokapirnasari Widyastuti, Arum WIJAYANTO WIJAYANTO Winarso, Widyo Wiwik Misaco Yuniarti YanRiska Venata Sembiring, YanRiska Venata Yeni Yulistanti Yohana Noradika Maharani Yulianto, Bernawan Yuniati, Ratna YUSMANI PRAYOGO Zainal Muttaqin zulfitra, Zulfitra