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Hasmiwati
Fakultas Kedokteran Universitas Andalas
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Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer Nabila Priscilla Putri; Hirowati Ali; Tofrizal Tofrizal; Eryati Darwin; Restu Susanti; Hasmiwati Hasmiwati
Jurnal Kesehatan Andalas Vol 12, No 2 (2023): Online July 2023
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v12i2.2142

Abstract

The increased production of beta-amyloid marks Alzheimer's disease. PPAR-γ shows involvement in Alzheimer's disease. PPAR-γ Gene is suspected to reduce beta-amyloid plaques and reduce inflammation and oxidative stress in Alzheimer's disease. Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) is expected to increase the PPAR-γ Gene expression. Objective:  To observed the PPAR-γ Gene expression in Alzheimer's Mice. Methods: The mice are induced using AlCl3 and were given MSC-WJ. This study was an experimental study with a post-test-only control group design on 18 samples of RNA from Alzheimer's mice that were divided into 3 groups. Negative control group (K-), positive control group (K+), and experimented group (P). The mean of PPAR-γ gene expression was obtained by comparing the PPAR-γ gene with the GAPDH gene. This study used semiquantitative methods using ImageJ. Data analysis was used with the Kruskal-Wallis test. It is significant if the value is <0,05. Results: The mean ratio of PPAR-γ gene expression that was obtained in K-, K+, and P were 0.12, 0.06, and 0.08, respectively. There were significant differences between each group, with a p-value of 0,023 (p<0,05). Conclusion: Giving MSC-WJ could increase the PPAR-γ gene expression. Further study using real-time PCR is highly needed to increase the MSC-WJ treatment in patients with Alzheimer's disease.Keywords:  Alzheimer disease, MSC-WJ, PPAR- γ
Time and Concentration-dependent Cytotoxicity of Piperine on Hela Cells Sri Oktavia; Arni Amir; Fatma Sri Wahyuni; Hasmiwati Hasmiwati
Majalah Kedokteran Andalas Vol 46, No 7 (2023): Supplementary December 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i7.p1174-1179.2023

Abstract

Piperine, the primary alkaloid of Piper nigrum and Piper longum, is believed to have anticancer properties. Although research on cervical cancer is still in its early stages, piperine's anticancer capabilities have been established in numerous types of cancer and have been shown to target pathways involved in the cell cycle and apoptosis. This study aims to determine how piperine administration time and concentration affect the viability of Hela cells. The MTT method was used to conduct this study. The concentration of piperine utilized was 1,000; 500; 250; 125, and 62.5 µg/mL, and it was administered for 24 and 48 hours. The IC50 values, obtained after incubation for 24 and 48 hours, were 711.21 µg/mL and 419.75 µg/mL, respectively. We may conclude that the viability of HeLa cells is influenced by piperine in a time- and concentration-dependent manner (p<0.05).
Deteksi Resistensi Piretroid Secara Bioassay dan Mutasi Gen kdr pada Aedes aegypti dan Aedes albopictus Puti Reno Diandra K.; Hasmiwati Hasmiwati; Beni Indra; Selfi Renita Rusjdi; Wirsma Arif Harahap; Nice Rachmawati Masnadi
Majalah Kedokteran Andalas Vol 46, No 7 (2023): Supplementary December 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i7.p1217-1228.2023

Abstract

Objective: The aim of this study is to detect the insecticide resistance by bioassay test and to detect the kdr mutation in Aedes aegypti and Aedes albopictus.Methods: This study was a narrative review that evaluated various articles related to detection of pyrethroid resistance by bioassay and mutation of the kdr gene in Ae. aegypti and Ae. albopictus sourced from PubMed, Google Scholar, and ProQuest database.Results: The results of the study review show that almost the entire population of Ae. aegypti and Ae. albopictus is already resistant to pyrethroid insecticide based on the bioassay detection using the WHO Susceptibility Test and CDC Bottle Assay protocols, and several variations of the kdr gene allele have been found, especially the V1016G, F1534C, and S989P genesConclusion: Ae. aegypti is resistant to pyrethroid insecticides, especially to deltamethrin and permethrin groups, Ae. albopictus are generally still in the susceptible status, and mutations in kdr gene are mostly found in the population of Ae. aegypti. The genotypes of the kdr that experienced many mutations are V1016G, F1534C, and S989P.
ISOLASI PIPERIN DARI LADA HITAM (Piper nigrum L.) DAN UJI KEMURNIANNYA Octavia, Maria Dona; Zaini, Erizal; Hasmiwati, Hasmiwati; Revila, Gusti
Jurnal Farmasi Higea Vol 16, No 1 (2024)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52689/higea.v16i1.621

Abstract

Piperin merupakan senyawa alkaloid yang terdapat di dalam lada hitam (Piper nigrum L). Pipierin diisolasi dari lada hitam dengan menggunakan metode sokletasi. Penelitian ini bertujuan untuk mendapatkan piperin hasil isolasi yang memiliki kemurnian tinggi. Uji kemurnian dilakukan secara kualitatif dan kuantitatif, meliputi monografi, kelarutan, analisa morfologi dengan menggunakan Scanning Electron Microscopy, KLT, panjang gelombang maksimum piperin, differensial scanning calorimetry (DSC), x-ray difrafraction (XRD), fourier transform-infra red (FT-IR), kromatografi cair kinerja tinggi (KCKT) dan penetapan kadar. Hasil isolasi piperin dibandingkan dengan piperin murni. Hasil rendemen piperin hasil isolasi diperoleh sebesar 2,156%. Hasil morfologi piperin isolasi menunjukkan serbuk kristal berbentuk batang berwarna kuning. Hasil analisis KLT diperoleh nilai Rf 0,314. Hasil Identifikasi ƛserapan maksimum adalah 341,40 nm. Hasil pemeriksaan titik lebur dengan DSC sebesar 132,579 OC. Hasil difraktogram menunjukkan pola difraktogram yang berbeda pada rentang sudut 2Ɵ, namun masih menunjukkan sifat kristal yang sama. Analisis spektrum FT- IR menunjukkan puncak transmitan yang sama pada bilangan gelombang. Hasil KCKT dengan fase gerak asetonitril: air (90:10) menunjukkan pola kromatogram yang sama dengan waktu retensi  adalah 3,967 menit dan memiliki kadar 100,686+2,636. Hasil uji kemurnian piperin isolasi secara kualitatif dan kuantitatif menunjukkan piperin isolasi memiliki kemurnian yang tinggi yang sama dengan piperin murni sebagai pembandingnya.
Edukasi Tentang Anemia dan Pemeriksaan Hb pada Ibu Hamil serta Pengobatan pada Masyarakat di Nagari Carocok Anau Kec.Tarusan Kab. Pesisir Selatan Elmatris Sy; Dessy Arisanti; Almurdi Almurdi; Endrinaldi Endrinaldi; Bobby Indra Utama; Dian Pertiwi; Rauza Sukma Rita; Zelly Dia Rofinda; Hasmiwati Hasmiwati; Aswiyanti Asri; Julizar Nazar; Mohamad Reza
Warta Pengabdian Andalas Vol 31 No 3 (2024)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.31.3.591-598.2024

Abstract

The majority of people of Nagari Carocok Anau Kec. Tarusan Kab. Pesisir Selatan worked as a fisherman, farmer, a small number of civil servants and market traders, as well as a housewife. Pregnant women in Nagari Carocok Anau, most of whom are housewives, still do not receive health checks such as haemoglobin (Hb) checks. This activity aims to increase knowledge among the public and health cadres regarding anaemia and Hb examination in pregnant women, as well as treatment. The health of pregnant women is checked by checking blood pressure, Hb and medication. This Hb examination is an effort to minimize abnormalities that can occur in pregnant women and fetuses due to low Hb when the mother is pregnant. The examination results showed that more than half of the pregnant women (71.43%) who attended were in the age range 18 – 35 years. Nearly half (42.86%) of pregnant women had Hb levels below the normal limit (11 mg/dl). For a more valid diagnosis, it is necessary to carry out quantitative analysis from the hospital laboratory so that follow-up can be carried out. Education about anaemia and Hb examinations in pregnant women can increase people's knowledge so that preventive measures can be carried out early. Health checks on the general public (blood pressure, Hb) and treatment were also carried out, it was found that the Hb results were within normal limits.
Berat Badan Bayi Usia 4-5 Bulan berdasarkan Kadar CU Dalam ASI Di Puskesmas Lumpo Pesisir Selatan Dewi Asmawati; Ellyza Nasrul; Hasmiwati Hasmiwati
JIK-JURNAL ILMU KESEHATAN Vol 5, No 1 (2021): JIK-April Volume 5 Nomor 1 Tahun 2021
Publisher : STIKes ALIFAH PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33757/jik.v5i1.376

Abstract

Tembaga (Cu) salah satu elemen penting dalam tubuh manusia, kelebihan dan kekurangan Cu dapat menyebabkan gangguan pada kesehatan tubuh. Salah satu kandungan yang terdapat dalam ASI adalah Cu. Tujuan penelitian ini adalah mengetahui berat badan bayi umur 4-5 bulan berdasarkan kadar Cu dalam ASI. Penelitian ini menggunakan metode Analisis Deskriptif dan pengambilan sampel menggunakan teknik simpel random sampling dengan jumlah sampel 40 responden pada bayi yang lahir aterm dan mendapat ASI secara eksklusif (4-5bulan). Pemeriksaan kadar Cu dalam ASI dilakukan di Laboratorium Kesehatan Daerah Sumatera Barat. Hasil penelitian menunjukkan kadar tembaga dalam ASI ibu didapatkan hasil (0,209 ± 0,07 mg/dL) dan sesuai dengan kadar normal sesuai kebutuhan bayi, dimana berat badan bayi terdapat peningkatan(6345 ± 847,30) gram, sesuai dengan usia 4-5 bulan. Selisih penambahan berat badan bayi dari lahir hingga usia 4-5 bulan adalah (3286,25 ± 904,900) gram,.Kesimpulan penelitian ini adalah kadar tembaga dalam ASI didapatkan sesuai kadar normal dan kebutuhan bayi. Keywords: Kadar tembaga, ASI, berat badan bayi
Description Of Foreign Bodies in The Esophagus in The ORL-HNS Section Of RSUP M. Djamil Padang Period 2020-2021 (COVID-19 Pandemic) Kevin Bryan Alghifari; Effy Huriyati; Hasmiwati Hasmiwati
The International Science of Health Journal Vol. 2 No. 4 (2024): December: The International Science of Health Journal
Publisher : Sekolah Tinggi Ilmu Kesehatan Kesdam IV, Diponegoro Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59680/ishel.v2i4.1570

Abstract

Esophageal foreign bodies are sharp or blunt objects or food that get stuck and pinched in the esophagus due to swallowing, either intentionally or unintentionally. Symptoms of esophageal foreign body obstruction depend on the size, shape, type of foreign body and the location of the foreign body. The purpose of this study was to determine how the description of foreign bodies in the esophagus in the ORL-HNS section of Dr. M. Djamil Hospital during the period 2020-2021 (Covid-19 Pandemic). This study is a descriptive study using a cross-sectional design. The sample of this study was a population that met the inclusion criteria using the total sampling method. This data collection used patient medical records conducted at the center of the medical record facility at Dr. M. Djamil Padang Hospital. The subjects of this study were patients diagnosed with esophageal foreign body ingestion in the ORL-HNS department of Dr. M. Djamil Hospital Padang in the period 2020-2021 (COVID-19 Pandemic). In this study, 22 samples were obtained that met the inclusion and exclusion criteria of the study. The results of this study found that the percentage of sex ratio between men was comparable to women with the largest age group, namely 0-<10 years (59.09%). There were most main complaints felt by patients, namely swallowing pain (31.81%) with the duration of foreign body in the esophagus mostly >12-24 hours (50%). The location of the most foreign bodies was in the introitus of the esophagus (72.73%) with the most types of foreign bodies coins (50%) and the most management performed was esophagoscopy (100%) and no complications were found in 17 cases (77.27%) of 22 cases. During the covid-19 pandemic there was a decrease in the number of patients swallowed by foreign bodies compared to before the covid-19 pandemic, there were no differences in age, complaints, location, tye of foreign body, duration, management and complications.
HUBUNGAN KADAR D-DIMER DENGAN DERAJAT KEPARAHAN PENYAKIT DAN ANGKA KEMATIAN PASIEN CORONAVIRUS DISEASE 2019 Trisuliandre, Muhammad Rizki; Herman, Deddy; Yulistini, Yulistini; Efrida, Efrida; Meinapuri, Malinda; Hasmiwati, Hasmiwati
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 1 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Maret 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i1.973

Abstract

Objective: This study aims to determine the relationship between D-dimer levels and the severity of the disease and the mortality rate of Coronavirus Disease 2019 patients treated at Dr. M. Djamil Padang General Hospital for the period June-August 2021. Method: This type of research is observational analytic using a retrospective cohort study design by taking a sample of 75 people through medical record data. Results: Most of the characteristics of the Coronavirus Disease 2019  patients were found in the age group of 56-65 years, male sex, most of them had an increased D-dimer value, and most of the patients did not die. This study found that there is a relationship between D-dimer levels and the severity of the disease and the mortality rate of Coronavirus Disease 2019 patients. Based on the Odd Ratio value obtained of 2.04 (1.53-2.71), it can be interpreted that people who have D -dimer increases have a risk of dying 2 times. Conclusion: There is a relationship between D-dimer levels and the severity of the disease and the mortality rate of 2019 Coronavirus Disease patients at Dr. M. Djamil Padang for the June-August 2021 period.
Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer Putri, Nabila Priscilla; Ali, Hirowati; Tofrizal, Tofrizal; Darwin, Eryati; Susanti, Restu; Hasmiwati, Hasmiwati
Jurnal Kesehatan Andalas Vol. 12 No. 2 (2023): Online July 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v12i2.2142

Abstract

The increased production of beta-amyloid marks Alzheimer's disease. PPAR-γ shows involvement in Alzheimer's disease. PPAR-γ Gene is suspected to reduce beta-amyloid plaques and reduce inflammation and oxidative stress in Alzheimer's disease. Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) is expected to increase the PPAR-γ Gene expression. Objective:  To observed the PPAR-γ Gene expression in Alzheimer's Mice. Methods: The mice are induced using AlCl3 and were given MSC-WJ. This study was an experimental study with a post-test-only control group design on 18 samples of RNA from Alzheimer's mice that were divided into 3 groups. Negative control group (K-), positive control group (K+), and experimented group (P). The mean of PPAR-γ gene expression was obtained by comparing the PPAR-γ gene with the GAPDH gene. This study used semiquantitative methods using ImageJ. Data analysis was used with the Kruskal-Wallis test. It is significant if the value is <0,05. Results: The mean ratio of PPAR-γ gene expression that was obtained in K-, K+, and P were 0.12, 0.06, and 0.08, respectively. There were significant differences between each group, with a p-value of 0,023 (p<0,05). Conclusion: Giving MSC-WJ could increase the PPAR-γ gene expression. Further study using real-time PCR is highly needed to increase the MSC-WJ treatment in patients with Alzheimer's disease.Keywords:  Alzheimer disease, MSC-WJ, PPAR- γ
Gambaran Antenatal Care Pada Pasien Preeklamsia dan Eklamsia Di RSUP Dr. M. Djamil Padang Periode 2021 Aisha, Fahra Audina; Ariadi, Ariadi; Hasmiwati, Hasmiwati
Jurnal Ilmu Kesehatan Indonesia Vol. 5 No. 4 (2024): Desember 2024
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v5i4.1037

Abstract

Latar Belakang: Salah satu penyebab utama morbiditas dan mortalitas ibu di dunia adalah preeklamsia. Bila terjadi kejang umum pada wanita preeklamsia, maka itu merupakan eklamsia. Beberapa faktor risiko preeklamsia dapat diketahui lebih awal dengan dilakukannya pemeriksaan antenatal care (ANC) secara teratur dan berkualitas. Objektif: Penelitian ini bertujuan untuk mengetahui gambaran ANC pada pasien preeklamsia dan eklamsia yang menjalani perawatan di RSUP Dr. M. Djamil Padang periode 2021 Metode: Penelitian ini adalah penelitian penelitian deskriptif retrospektif. Penelitian ini dilakukan di rekam medis RSUP Dr. M. Djamil Padang pada bulan Januari 2022 – Oktober 2022. Penelitian ini menggunakan teknik total sampling dengan jumlah sampel sebanyak 42 pasien preeklamsia dan eklamsia. Hasil: Sebagian besar sampel berusia 20-35 tahun 73.8%, pendidikan 52,4% SMA, sampel ibu rumah tangga sebanyak 73.8%. Terdapat 78.6% dengan diagnosis preeklamsia dan 21.4% dengan diagnosis eklamsia. Mayoritas sampel tidak memenuhi kunjungan antenatal K4 yaitu sebanyak 61.9%. Sampel sebagian besar telah memenuhi standar 7T yaitu sebanyak 88.1% Kesimpulan: Pasien preeklamsia dan eklamsia di RSUP Dr. M. Djamil mayoritas berusia 20-35 tahun, pendidikan terakhir SMA, dan mayoritas merupakan ibu rumah tangga dengan sebaran kasus 78,6% preeklamsia dan 21,4% eklamsia. Gambaran ANC pada seluruh pasien berdasarkan kunjungan ANC yaitu lebih banyak yang tidak memenuhi minimal kunjungan K4. Berdasarkan standar 7T ANC yang telah memenuhi standar 7T sebanyak 88.1%.
Co-Authors Abdiana Abdiana, Abdiana Adrial Adrial Adrial Adrial Adrial, Adrial Afriwardi Afriwardi Afrizal Afrizal Agustini, Cynthia Aisha, Fahra Audina Alioes , Yustini Almurdi Almurdi Amir, Arni Andani Eka Putra Ariadi, Ariadi Arina Widya Murni Arni Amir Arni Amir Aswiyanti Asri Aswiyanti Asri Beni Indra, Beni Bobby Indra Utama Citra Manela Daan Khambri Dahelmi Dahelmi Deddy Saputra Delmi Sulastri Desmawati Desmawati Desmiwarti Dessy Arisanti Dessy Arisanti Dewi Asmawati Dia Rofinda, Zelly Dia Dian Pertiwi Dian Pertiwi Diandra K., Puti Reno Effy Huriyati Efrida Efrida Eka Nofita Eka Novita Ellyza Nasrul Elmatris Sy Endrinaldi Endrinaldi Endrinaldi Erizal Zaini Erlina Rustam Erlina Rustam Eryati Darwin Fadrian, Fadrian Faisaelia, Rezka Nanda Fatma Sri Wahyuni Firdawati Firdawati Fortuna, Fory Gestina Aliska Ginting, Desi Scorpinasari Gusti Rati Hardisman Harminarti, dr Nora Hendriani, Intania Dwiputri Hendriati, Hendriati Henmaidi Henmaidi Henny Herwina Herman, Deddy Hirowati Ali Hirowati Ali, Hirowati Husna Yetti Husnil Wardiyah Huvaid, Sevilla Ukhtil Ida Rahmah Burhan Ilmiawati, Ilmiawati Intan, Shinta Ayu Julizar Julizar Julizar Nazar Karmia, Hudila Rifa Kevin Bryan Alghifari Kharisma Putra Kharisma Putra D Lestari, Rahmi Linosefa Linosefa Luois Joseph Joseph Mairawita, Mairawita Malinda Meinapuri Maria Dona Octavia Marwin, Fikri Alhafizd Masnadi, Nice Rachmawati Mohamad Reza Mohamad Reza Muhamad Diva Caesar Muhammad Nazri Janra Nabila Priscilla Putri Nesa Pratiwi Nice Rachmawati Masnadi Nita Afriani Noverika Windasari Noza Hilbertina, Noza Nur Afrainin Syah Nur Indrawaty Lipoeto Nurhalimah Juneldi Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati, Nurhayati Nursyirwan Effendi, Nursyirwan Nurul Badriyah Nuzulia Irawati Oktavia, Sri Puti Reno Diandra K. Putra, Fachri Putri, Nabila Priscilla Putri, Salsa Utami Raden Mohamad Herdian Bhakti Rahmatini . Ramadhan, Mario Arya Ratu Fawwaz Efendi Rauza Sukma Rita Resti Rahayu Restu Susanti Revila, Gusti Rifa, Farras Zahra Rika Susanti Rizanda Machmud Rizka Karima Husfa Rosfita Rasyid Roslaili Rasyid Rozi Sastra Purna Rustam, Rony Savitri, Melvinia Selfi Renita Rusdji Selfi Renita Rusjdi Selfi Renita Rusjdi, Selfi Siti Nurhajjah Sri Oktavia Suharti , Netti Sukri Rahman Syamel Muhammad Taufik Ramadhani Tiya Taslisia Tofrizal Tofrizal Tofrizal Trisuliandre, Muhammad Rizki Vaulline Basyir Vilza Maharani Syahnel Wirsma Arif Harahap Wirsma Arif Harahap Yenita . Yenita Yenita Yoseph De Nachs Yulistini, Yulistini Yuniar Lestari Zelly Dia Rofinda