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Isolation and Identification of Genus Salmonella and Shigella from Sumatran Orangutan (Pongo abelii) Feces in Orangutan Reintroduction Center, Jantho Erdiansyah Rahmi; Dina Agustina; Faisal Jamin
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i1.3316

Abstract

This study was done to identified bacteria of genus Salmonella and Shigella from sumatran orangutan (Pongo abelii) feces. Nine semi wild orangutan fecal samples were obtained in Orangutan Reintroduction Center, Cagar Alam Pinus Jantho, Aceh Besar. The samples were processed using Carter methode. The selective media used were Salmonella Shigella Agar (SSA), Eosin Methylen Blue (EMB), and MacConkey Agar. The separated colonies which grawn in SSA media were observed for their morphology, and followed by Gram staining methode then continued with biochemical test such as Indol, Methyl Red (MR), Voges Proskauer (VP), Sulfid Indol Motility (SIM), Simmons Citrate (SC), and sugar test such as glucose, sucrose, lactose, and manitol. From nine samples, one orangutan was infected with Salmonella and another one was infected with Shigella. All of the samples were identified with Escherichia. In Conclusion, orangutan lived at Reintroduction Center, Jantho infected with Salmonella and Shigella bacteria.Key words: feces, orangutan, Salmonella, Shigella
4. Case Study: Gallstones (Cholelithiasis) in Long-tailed Macaques (Macaca fascicularis) J. Susilo; B. Tri Wibowo; Y. Tinasari; M. Nur Salim; Dian Masyitha; Erdiansyah Rahmi; M. Isa; Herialfian Herialfian; Fakhrurrazi Fakhrurrazi; T. Fadrial Karmil; Fadli A. Gani
Jurnal Medika Veterinaria Vol 15, No 2 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i2.25204

Abstract

The purpose of this case study is to diagnose the cause of death of the long-tailed macaque (Macaca fascicularis). Two cadaver of Macaca fascicularis were received by the Lampung Veterinary Center from a customer. Necropsy was carried out at the Pathology Laboratory of the Lampung Veterinary Center by observing macroscopic changes. The samples taken were then observed by gram staining in the Bacteriology laboratory and hematoxylin-eosin staining at the Pathology laboratory. The results of macroscopic observations showed an enlarged gallbladder with a length of 4 cm and a width of 2 cm, blackish in color. Gallbladder surgery found 15 gallstones measuring 2 mm in diameter, brownish-colored bile, fibrin clots, and blood. The native gram stain of bile showed several types of rod-shaped bacteria form and gram-negative cocci (eosinophilic), and gram-positive rods (basophilic). Specific histopathological changes in the ductus choleductus are multifocal neutrophil inflammatory cell infiltration, enlarged gallbladder lumen filled with multi-bacterial, cell debris, fibrin, and erythrocytes. Multifocal hepatitis occurs by neutrophils, multibacterial, and fibrin. It can be concluded that the causes of death of Macaca fascicularis were cholelithiasis and hepatitis.
PREVALENSI PERUBAHAN PERILAKU ANJING LOKAL (Canis familiaris) JANTAN YANG DIKANDANGKAN DENGAN PRINSIP KESEJAHTERAAN HEWAN SELAMA 60 HARI (Prevalence of Behavioural Changes in Male Local Dog (Canis familiaris) Caged Based on Animal Welfare Principles for 60 Days) Nofri Alfi; Teuku Reza Ferasyi; Erdiansyah Rahmi; Mulyadi Adam; Idawati Nasution; Ismail Ismail
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3823

Abstract

The aim of this research was to determine the prevalence of behavioral changes of male local dog caged based on animal welfare principles. A total of three male local dogs was used as the experiment object. Dog behavior was observed using focal animal sampling method. Dogs were caged for 60 days, provided with sufficient food and water ad libitum. The dogs were identified based on their color and body size. Their behavior were observed three times each days in the morning, midday, and afternoon with 3 periods: period I (day 1 to day 20), period II (day 21 to day 40), and period III (day 41 to day 60). The observation was carried out for 60 days with allocation time was 30 minutes/ each observations. The result obtained indicated that the prevalence of behavior in dogs during the first period was dominated by sitting and sleeping. In addition, they were showed the behavior of running, walking, playing, sniffing, and eating. During this period, they were also expressed of stress behavior, such as barking, digging, backward/hiding, biting, and climbing. In the second and third periods of observation, the behaviors of sitting and sleeping are increased while behaviors of stress are reduced. Based on this results, it can be concluded that local dogs indicate stress behavior during the first 20 days caged, then they become more calm during the following days when animal welfare principle is fulfilled.Key words: local dog, dog behavior, animal welfare
GAMBARAN HISTOLOGI KELENJAR INTESTINAL PADA DUODENUM AYAM KAMPUNG (Gallus domesticus), MERPATI (Columba domesticus) DAN BEBEK (Anser anser domesticus)(Histological Feature of Intestinal Glands of Native Chicken (Gallus domesticus), Pigeon (Columba domesticus), and Duck (Anser anser domesticus)) Zainuddin Zainuddin; Dian Masyita; Sarayulis Sarayulis; M. Jalaluddin; Erdiansyah Rahmi; Idawati Nasution
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4028

Abstract

The aim of this research was to observe intestinal glands histology of duodenum from native chicken, pigeon, and duck. Samples used are duodenum from 3 native chickens, 3 pigeons, and 3 ducks with undifferentiated of sex and weight. Fowl was slaughtered, duodenum were taken out then proceeded for histology method using parraffination and stained with hematoxylin-eosin. Histological observation include the density and morphology of intestinal glands. Data were analyzed descriptively. The results showed that the intestinal glands histology differ among native chicken, pigeon, and duck but the morphology of intestinal gland was similar that was simple tubular. The number of intestinal glands in native chicken and pigeon almost similar and found in high density but lower than that found in pigeon. This difference assumed to be correlated with type and concentration of feed.
11. Study of Cat’s Welfare Kept at Pet Shops in Gajah Mada Mal Central Jakarta Putri Aisyah Hanifah; Mulyadi Adam; Roslizawaty Roslizawaty; Abdullah Hamzah; Erdiansyah Rahmi; T. Armansyah TR
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.3743

Abstract

The aim of this study was to find out correlation between cat’s welfare and caring management in a number of cats kept at pet shops in Gajah Mada mal Central Jakarta, from January to February 2016. This study used a questionnaire with ratings based on five aspects of animal welfare (freedom from hunger and thirst, freedom from discomfort, freedom from pain, injury, and disease,  freedom from fear and distress, and freedom to express normal behavior) and through direct observation. Data was analyzed by Correlation Test Chi Square with 95% confidence interval. The result shows that there is a significant correlation (P0,05) in freedom from hunger and thirst with freedom from fear and distress aspects, and there is no correlation (P0,05) between caring management and animal welfare standart in freedom from pain, injury, and disease aspects,  freedom from discomfort aspect, and freedom to express normal behavior with caring management aspects.
2. DETEKSI LEUKOSIT URINE ORANGUTAN SUMATERA (Pongo abelii) MENGGUNAKAN STRIP TEST SEMIKUANTITATIF DI PUSAT REINTRODUKSI JANTHO (Detection of Leucocyte on Sumatran Orangutan (Pongo abelii) Urine Using Semiquantitative Strip Test in Jantho’s Reintroduction Center) Meuthya Sr; Rusli Rusli; Erdiansyah Rahmi
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i2.4384

Abstract

The aim of this study was to find out the information about sumatran orangutan urine leucocyte as a medical record of them at Cagar Alam Pinus Jantho Reintroduction Center, Aceh Besar. Urine samples were collected from ten sumatran orangutans which come from forest, around cages, and in the cage. The urine was collected in the morning when orangutan woke up and left the nest or before first feeding for orangutans which were in the cages. The replication test was done six times within one month. Urine examination was carried out directly after urine collection by soaking the strip test in 5 ml urine for 120 second. The data was analyzed by qualitative descriptive method and the result was semiquantitative based on the discoloration scale: negative, trace (±15 leucocyte/μL), +1 (70 leucocyte/μL), +2 (125 leucocyte/μL), and +3 (500 leucocyte/μL). The result showed that the average of leucocyte from 4 orangutans which were in the cages (Ego, Wenda, Miky, and Ontong) was ±15 leucocyte/μL, while Ayu Ting Ting for the first test had the highest level of leucocyte (500 leucocyte/μL) then decreased on the next test. Coty, Ruben, Yusniar (arround the cages) had variative leucocyte values every week. Two orangutans in the forest (Kamelia and Brokoli) had negative value for urine leucoyte.Key words: leucocyte, sumatran orangutan, strip test, urine
Analysis of the Role of Keeper as a Risk Factor for Infection of Gastrointestinal Nematodes in Sumatran Orangutan in the Re-introduction Station of Jantho Ali Murtala; T. Reza Ferasyi; Erdiansyah Rahmi
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i2.3363

Abstract

This study was aimed to identify the role of keeper as a risk factor for the infection of gastrointestinal nematodes on orangutans in Jantho Re-introduction Station. This study was conducted using a census method. A number of 8 orangutan’s keepers were involved as respondent. The data was collected by interview using a structural questionnaire. In addition, feces of 6 orangutans caged in this location were taken as sample. The feces sample was then examined for gastrointestinal nematodes using native and centrifugation methods in Parasitology Laboratory, Veterinary Medicine Faculty, Syiah Kuala University. The data obtained from this study were analyzed descriptively. The results of study by questionnaire showed that a possible potential risk factor for the infection of gastrointestinal nematode to orangutans in Jantho Re-introduction Station is the keeper avoids using gloves when supplying food to the animals. Then, the result of laboratory examination showed that it was confirmed that the feces of orangutans infected with gastrointestinal nematodes, which the prevalence of 16.5% for infection by Strongiloides sp. In conclusion, orangutans caged in Jantho Re-introduction Station are in a low infection of gastrointestinal nematodes of Strongiloides sp., and the keeper shows a good behavior to prevent gastrointestinal nematodes infection to orangutan.Key words: keeper, Sumateran orangutan, Pongo abelii, Strongyloides sp.
7. Density of Lumbal Vertebrae Bone Ovariectomized Rat (Rattus Norvegicus) Given the Extract Sipatah – patah ( Cissus quadrangularis Salisb ) Putri Dewi; Mustafa Sabri; Erdiansyah Rahmi; M Jalaluddin; Nuzul Asmilia; Al Azhar
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i1.4117

Abstract

The aims of this research was to determine the density level of lumbal vertebrae on ovariectomized rats administered with Cissus qudrangula Salisb stem extract with various doses. In this research twelve female rats were randomizedly divided into 4 groups with 3 treatment repetition. K1-groups were ovariectomized rats without Cissus quadrangula Salisb trunct extract administration; K2, K3, and K4, were ovariectomized and given with Cissus quadrangula Salisb extract  with 500 mg/kg/Bw, 700 mg/kg/Bw, and 900 mg/kg/Bw as doses perday for 30 days. On day-31, rats were euthanized using chloroform and the os lumbal-5 were collected, followed with histological preparations. Research parameters were the density of active osteoblasts, passive osteoblasts, and osteoclasts. The results showed that the damage in K1 was clearly marked with the high number of osteoclasts, K2 and K3 indicate lower osteoclasts levels than K1 and had repaired bone structure characterized with active cuboidal shaped  osteoblast  activity and passive flat shaped osteoblasts and K4 showed increasing  level of active osteoblasts. The administration of Cissus quadrangula Salisb stem extract at 900 mg/kg/bw for 30 days showed bone repair due to ovariectomy indicated with high level of active osteoblasts.
Histology and Histomorphometry of The Skin of Gallus gallus domesticus, Anas platyrhynchos and Columba livia on The Caudal Region Masyitha, Dian; Zainuddin, Zainuddin; Iskandar, Cut Dahlia; Rahmi, Erdiansyah; Sayuti, Arman; Nursalim, M; Ningrum, Aulia Putri Wahyu
Serambi Saintia : Jurnal Sains dan Aplikasi Vol 11, No 2 (2023): Serambi Saintia
Publisher : Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/jss.v11i2.7177

Abstract

The skin is an organ that functions to coat, protect and wrap the surface of the body and can maintain the balance of fluids in the body, including in birds. Aves skin can also provide a thermal insulation function. In general, the skin layers of aves consist of the epidermis, dermis and hypodermis. This study aimed to describe the histological structure and histomorphometric skin of native chickens (Gallus gallus domesticus), ducks (Anas platyrhynchos) and pigeons (Columba livia) in the caudal region. The samples used were six individuals each of. The skin of the caudal region of the sample is then processed, started from preparation to a histological preparation with a thickness of 5 m, followed by Haematoxylin-Eosin (HE) staining. The results showed that the histological structure of the skin of the three types of aves consisted of the epidermis, dermis and hypodermis with a thinner thickness than the skin of mammals. The measured histomorphometric data showed that the epidermal layer thickness of the skin of the caudal region of native chickens, ducks and pigeons was not significantly different (P0.05), there was a significant difference (P0.05) in the thickness of the dermis and hypodermis layers. It was concluded there was no difference in the histological structure of the skin of native chickens, ducks and pigeons, the skin histomorphometry of the caudal region of native chickens, the dermis and hypodermis were thicker than those of ducks and pigeons.Keywords: Skin, aves, caudal, histology, histomorphometry.
Histologi, Histomorfometri, dan Histokimia Usus Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas Bagus Dwijayanti; Erdiansyah Rahmi; Ummu Balqis; Fitriani Fitriani; Dian Masyitha; Dwinna Aliza; Muslim Akmal
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.16012

Abstract

ABSTRACT. Usus adalah salah satu organ pencernaan yang berperan penting pada proses absorpsi nutrisi sebagai sumber energi. Penelitian ini bertujuan mengetahui histologi, histomorfometri dan histokimia sebaran glikogen pada usus ayam buras (Gallus gallus domesticus) sebelum dan setelah menetas menggunakan pewarnaan Haematoksilin-Eosin (HE) dan pewarnaan Periodic Acid Schiff (PAS). Sampel penelitian terdiri atas empat kelompok tingkat umur berbeda, setiap kelompok berjumlah enam usus ayam. Hasil penelitian menunjukkan perbedaan nyata (P0,05) morfometri usus ayam buras secara makroskopis. Secara histologis pada setiap perkembangan sudah terlihat lapisan usus yang terdiri atas tunika mukosa, submukosa, muskularis, dan serosa. Pada tunika mukosa, didapati tiga lamina yaitu lamina epitelia, propria, dan muskularis mukosa yang mulai teramati pada masa inkubasi hari ke-14. Lamina epitelia berupa sel epitel silindris selapis dengan mikrovili, lamina propria berupa jaringan ikat, dan lamina muskularis mukosa berupa berkas otot polos. Pada tunika mukosa dijumpai adanya sel Goblet dan kripta Liberkuhn yang teramati pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. Hasil histomorfometri ketebalan lapisan usus, panjang vili, lebar vili, dan diameter lumen usus ayam buras pada masa inkubasi dan setelah menetas menunjukkan perbedaan nyata (P0,05). Temuan reaksi positif kuat terhadap pewarnaan PAS dijumpai pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7 dan positif lemah pada masa inkubasi hari ke-14. Dapat disimpulkan bahwa terdapat korelasi antara perkembangan usus ayam secara makroskopis, histomorfometri ketebalan tunika dan vili, serta sebaran reaksi positif kuat terhadap pewarnaan PAS pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. (Histological, histomorphometrical, and histochemical of intestine on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRAK. The intestines are one of the digestive organ that play an important role in the nutrient absorption process as an energy source. The aim of this study was to determine the histomorphometric and histochemical of glycogen distribution in the intestines of native chickens pre and post hatching periods using Haematoxylin-Eosin (HE) staining and Periodic Acid Schiff (PAS) staining. The samples of this study was consisted of four different age groups, each group consisting of six chicken intestines. The results showed a significant difference (P0.05) on macroscopic morphometry of the native chickens intestine. Histologically in each group has been found the intestines consisting of mucosa, submucous, muscularis, and serous tunic. In the mucosal tunic, there were three layers; ephitelial, propria, and muscularial mucous can be observed during the 14th day of incubation period. Ephitelial layer in the form of cylindrical epithelial cells with microvilli, proprial layer in the form of connective tissue, and layer of the muscularis mucosa form smooth muscle thread. In the mucosal tunic, there was Goblet cells and Liberkuhn crypts that can be observed during the 20th day incubation period and 7th day post hatch. Histomorphometry results of intestinal lining thickness, villi length, villi width, and lumen diameter of native chicken intestine during incubation and post hatching showed significant differences (P0.05). The findings of a strong positive reaction to the staining of PAS were found on the incubation period of the 20th day and post hatching the 7th day and positively weak on the incubation period of the 14th day. It can be concluded that there is a correlation between macroscopic development of chicken intestine, histomorphometric of tunica and villous thickness, as well as the distribution of strong positive reactions to the staining of PAS during the incubation period of the 20th day and post hatching the 7th day.
Co-Authors A, Aulanniam A.A. Ketut Agung Cahyawan W Abdullah Hamzah Abdullah Hamzah, Abdullah Al Azhar Ali Murtala Ali, Ihfan Khaidir Amalia Sutriana Amiruddin . Amiruddin A Amiruddin Amiruddin Arman Sayuti Arman Sayuti Aulanni'am, Aulanni'am Aulia, Usma Awaluddin Awaluddin Azhari Azhari B. Tri Wibowo Bagus Dwijayanti Basuki B. Purnomo Basuki B. Purnomo budianto panjaitan Cut Dahlia Iskandar Cut Nila Thasmi Darmawi Darmawi Dasrul Dasrul Daulay, Lingga Surya Maret Dian Masyita Dian Masyitha Dian Masyitha Dian Masyitha Dina Agustina Dwinna Aliza Dwinna Aliza Dwinna Aliza Erina Erina Fachreza Oktavian Syahputri Fadli A. Gani Faisal Jamin Faisal Mustafa Fakhrurrazi Fakhrurrazi Febrianto, Niko Fitriani Fitriani Gani, Fadli A Gholib Gholib Gholib Gholib, Gholib Ginta Riady Habiburrahman Habiburrahman, Habiburrahman Hafizuddin Hafizuddin Hamdan Hamdan Hamny Hamny Hamny Sofyan Henni Vanda Herialfian Herialfian Hery Wijayanto Idawati Nasution Idawati Nasution Intan Regina Iqbar Iqbar Ismail Ismail J. Susilo Juli Melia Juli Melia Khairul Rizal Kusdiantoro Mohamad Lian Varis Riandi Lingga Surya Maret Daulay M Jalaluddin M Nur Salim M Nur Salim M. Aris Widodo M. Aris Widodo M. Hasan M. Isa M. Isa Mahdi Abrar Mardiana Mardiana Meuthya Sr Muhammad Hambal Muhammad Hanafiah Muhammad Isa Muhammad Isa Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Mulyadi Adam Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Mustafa Sabri NA, Zuhrawaty Niko Febrianto Ningrum, Aulia Putri Wahyu Nofri Alfi NURLIANA NURLIANA Nursalim, M Nuzul Asmilia Nuzul Asmilia Putri Aisyah Hanifah Putri Dewi Qathrinnada Ramadhana Rahmat Nazif Ramadhan, Rezky Razali Razali Reza Perdana Putra Rinidar Rinidar Rita Andini Ritonga, Mudhita Zikkrullah Ritonga, Mudhita Zikrullah Rizal, Khairul roslizawaty roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Rusli Rusli Rusli Sulaiman Safika S, Safika Sarayulis Sarayulis Sembiring, Rinawati Siska Pratiwi Siswandy Siswandy Siti Aisyah Siti Hajar Sri Wahyuni Sri Wahyuni Sugito - Sugito Sugito Sultan Fadhilla Taqwa Sutiman B. Sumitro Syafruddin s Syafruddin S Syafruddin Syafruddin Syafruddin Syafruddin T Armansyah TR T. Armansyah T. Armansyah T. Armansyah TR T. Fadrial Karmil T. Reza Ferasyi Tanjung, Fitri Aisah Taufan Hidayat Teuku Reza Ferasyi Tongku Nizwan Siregar TR, T Armansyah Tri Wahyu Pangestiningsih Triva Murtina Lubis Ummu Balqis Wahono Esthi Prasetyaningtyas Wahyuni Azani Wardhani, Lailia Dwi Kusuma Wenny Novita Sari Wisnu Nurcahyo Y. Tinasari Yandi Syahputra Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin, Zainuddin Zico, Hanifa Zuhrawati Zuhrawati Zuhrawati Zuhrawati, Zuhrawati Zuhrawaty Zuhrawaty Zulkifli, Baidillah