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Synthesis of Two Analogues of Xylapeptide A and Their Potency as New Antimicrobial Agent Maharani, Rani; Muchlis, Handi Nugraha; Hidayat, Ace Tatang; Al-Anshori, Jamaludin; Nurlelasari, Nurlelasari; Harneti, Desi; Mayanti, Tri; Farabi, Kindi; Supratman, Unang
Indonesian Journal of Chemistry Vol 25, No 5 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.100353

Abstract

Xylapeptide A, derived from the fungus Xylaria sp. x Sophora tonkinensis, exhibits potent and selective antimicrobial properties. Our research group has successfully synthesized xylapeptide A. In our recent work, two xylapeptide A analogues (An1 and An2) were synthesized using a combination of solid- and solution-phase synthesis methods. The linear precursors of An1 and An2 were synthesized on 2-CTC resin with the Fmoc strategy. The coupling reagents HBTU/HOBt and HATU/HOAt were employed. Subsequently, the linear precursor was cleaved from the resin using either 20% TFA or a TFE mixture, and then cyclized in solution phase with HBTU. The synthesized products were purified using semi-preparative RP-HPLC, giving the percent yields 16% for An1 and 12% for An2. Both compounds were then characterized by HR-ToF-MS, 1H- and 13C-NMR. The synthesized xylapeptide A and its analogues were evaluated against Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Candida albicans. The result showed that An2, possessing arginine residue, exhibited higher activity compared to xylapeptide A and An1. This research suggests that xylapeptide A analogues hold great promise as novel antimicrobial agents.
Ethanol extract of mangosteen (Garcinia Mangostana Linn) peel effect in inhibiting the growth of human tongue cancer cells Supri’s Clone 1, invitro Suanto, Edi; Oewen, Roosje Rosita; Sasmita, Inne Suherna; Supriatno, S.; Supratman, Unang
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.616 KB) | DOI: 10.24198/pjd.vol23no2.14022

Abstract

The incidence of tongue cancer in Indonesia reached 1.01% of all cancers and 42% of oral cavity cancer. Tongue cancer therapies including chemotherapy, radiotherapy, surgery, and all three combined therapy. Search for anti-cancer drugs currently switched on herbal plants, one of which is the mangosteen. Has the properties of mangosteen peel extract inhibited the growth of cancer cells. The purpose of the study, obtain IC50 of ethanol extract of mangosteen peel in inhibiting the growth of human tongue cancer cells SP-C1. Research carried out on 96 preparations of human tongue cancer SP-C1 were incubated with ethanol extract of mangosteen peel, preparations were classified in two groups of incubation time (24 hours and 48 hours) and each group will be given preferential treatment over 6 randomly different concentrations: 0 (control), 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL and 1000 μg/mL. Model experiments were 2 x 6 factorial experiment with eight replication for each cell. Test results with ANAVA, incubation (24 and 48 hour) SP-tongue cancer cells with various concentrations of C1 ethanol extract of mangosteen peel gives a highly significant, indicating differences cancer cell growth inhibition. Incubation time factor showed the long incubation effect on cancer cell growth inhibition. Furthermore, by Newman Keuls test, showed 500μg/mL concentrations of 24-hour incubation had the best effect. Conclusion of the study of ethanol extract of mangosteen peel could achieve with IC50 values of cell growth resistance 50.3% at a concentration of 500 μg/mL and an incubation time of 24 hours.
STUDI EKSTRAK n-HEKSANA DARI KULIT BATANG KANDIS HUTAN (Garcinia cymosa) Darwati; Nurlelasari; Tri Mayanti; Unang Supratman
Jurnal Penelitian Hasil Hutan Vol. 39 No. 3 (2021): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2021.39.3.148-154

Abstract

Plants of Garcinia genera (Fam: Guttiferae) that grows in Indonesia's tropical forests are potential of triterpenoidcompounds contents. Garcinia cymosa has been reported as the main source of triterpenoid compounds whichprovided useful biological activity such as anti-inflammatory, antibacterial, antifungal, antioxidant, cytotoxic, andanti-HIV. Currently, data and information of triterpenoid compounds in the G. cymosa is relatively low. Thispaper studies triterpenoid compound of G. cymosa. This compound was obtained from macerated G. cymosa stembark using n-hexane solvent. G. cymosa bark was macerated using n-hexsana solution, which was them separatedand purified by chromatography method, to produce pure isolate in the form of white needle crystals (10.8 mg). Thechemical structure was then determmined by using spectroscopy methods of IR, 1D-NMR, 2D-NMR, and massspectroscopy compared with published structure. Result show friedelin compound was succesfully isolated from extractedn-heksana of G. cymosa bark.
Eudesman-Type Sesquiterpenoids from Stem Bark Dysoxylum gaudichaudianum and Cytotoxic Evaluation Against Human HeLa Cervical Cancer Maira, Faizah; Naini, Al Arofatus; Mayanti, Tri; Farabi, Kindi; Fajriah, Sofa; Retnowati, Rurini; Supratman, Unang
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 2, November 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i2.46788

Abstract

Two eudesmane-type sesquiterpenoids were isolated from the stem bark of Dysoxylum gaudichaudianum: 6α-hydroxy-eudesm-4(15)-en-1-one (1) and eudesm-4(15),7-dien-1β-ol (2). This study represents the first report of these compounds not only from D. gaudichaudianum but also from the genus Dysoxylum. The cytotoxic potential of two sesquiterpenoids was assessed against human cervical carcinoma (HeLa) cells employing the Resazurin-based PrestoBlue assay. Using cisplatin as a positive control, compound 1 exhibited moderate cytotoxicity with an IC₅₀ of 28.04 µM, whereas compound 2 showed comparatively weaker activity, with an IC₅₀ of 58.37 µM. Their structures were elucidated through comprehensive spectroscopic analyses, including HR-ESI-MS, ¹H NMR, and ¹³C NMR. Structure–activity relationship analysis indicates that hydroxylation at C-6 enhances cytotoxic activity, whereas the C-6/C-7 olefinic moiety reduces potency, likely due to increased molecular rigidity, highlighting key structural features for activity modulation in the eudesmane scaffold.
The Phenolic Compounds Isolated from Myristica fragrans and Their Cytotoxic Effects on B16-F10 Melanoma Cancer Cell Lines Hasbilla, Raihan Fathurrahman; Riyadi, Sandra Amalia; Safriansyah, Wahyu; Hidayat, Ace Tatang; Susianti, Susianti; Salam, Supriyanto; Lesmana, Ronny; Retnowati, Rurini; Supratman, Unang
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.16265

Abstract

Phenolic compounds are a major type of secondary metabolite found in plants. These compounds are synthesized through shikimic and phenylpropanoid pathways, resulting in the formation of numerous unique structures and bioactivities. In addition, a significant amount has been reported in nutmeg, an endemic plant of Indonesia, which has been widely used in traditional medicine. A previous study also revealed that ethyl acetate extract of the plant has notable cytotoxic effects against melanoma B16-F10. Therefore, the purpose of this study is to isolate and evaluate phenolic compounds in nutmeg for their potential to inhibit B16-F10 melanoma cancer cell growth. The seeds extract of nutmeg was separated by various chromatographic techniques to yield a total of five compounds, which were identified through spectroscopic analysis (HR-TOF-ESI-MS, IR, and NMR) as well as comparison with literature. The compounds 1-5 were identified as (+)-veraguensin (1), 3',4',5'-trimethoxycinnamyl alcohol (2), (+)-galbegin (3), (-)-polysphorin (4), and 7-methoxycoumarin (5). Cytotoxic effects were then assayed against B16-F10 melanoma cell lines using the Resazurin method. Furthermore, compound 1 displayed the highest cytotoxic activity, with an IC50 value of 112.71 µM.
Kaempferol and Quercetin Isolated from The Leaves of Atingia Excelsa to Arrest Cell Cycle in G0/G1 Phase Human Tongue Cancer SP-C1 Cell Lines Risyandi Anwar; Arlette Setiawan; Supriatno; Unang Supratman
Denta Journal Kedokteran Gigi Vol 14 No 1 (2020): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i1.4

Abstract

The leaves of Altingia excelsa were found to strongly inhibit SP-C1 human tongue cancer cell lines. This study was focused on identifying the antiproliferative compound found in A. excelsa leaves and assessing its mechanism of action. The active compound was isolated using column chromatography and identified by the spectroscopic method and was also tested for its anti-proliferative properties and the cell cycle analysis in SP-C1 cells by flowcytometry analysis. This work resulted in the isolation of a flavonoid, which was identified to be kaempferol and quercetin. The compounds inhibited SP-C1 cell proliferation in a time- and dose-dependent manner with IC50 values of 0.72 µg/mL and 0. 70 µg/mL for the 24 hours treatments, respectively. Furthermore, the flowcytometry analysis suggested that the compounds exerted its anticancer activities by inhibiting cell cycle. These results suggested that compounds found in A. excelsa providies a basis for its potential use in cancer disease management.
Penta- and Tetra-cyclic Triterpenoids from Aglaia cucullata Stembark and Cytotoxicity against Cancer and Normal Cells Farabi, Kindi; Safriansyah, Wahyu; Harneti, Desi; Kuncoro, Hadi; Fajriah, Sofa; Supratman, Unang
Journal of Mathematical and Fundamental Sciences Vol. 57 No. 2 (2025)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2025.57.2.1

Abstract

Three triterpenoid compounds, belonging to the penta- and tetracyclic groups, were successively isolated from the stembark of Aglaia cucullata and identified as b-amyron (1), dammaradienon (2), and cabralealactone (3). After extensive extraction, chromatographic separation and purification, compounds 1 to 3 were gained. Spectroscopic analysis in addition to HRMS, FTIR, 1D and 2D NMR analysis were utilized to determine the chemical structure. After that, compounds 1 to 3 were tested against breast cancer, melanoma as well as normal kidney cells (MCF-7, B16-F10, and CV-1, respectively). The resulted cytotoxicity test revealed that compound 1 had the best cytotoxicity against all cells compared with the other isolated compounds. This result suggests that the pentacyclic triterpenoid core in compound 1 increases cytotoxicity compared with a tetracyclic skeleton.
Studi In Silico Aktivitas Senyawa Steroid Terhadap Antikanker Payudara Menggunakan Estrogen Alfa (ER-α) Nurlelasari, Nurlelasari; Widyana, Almas; Julaeha, Euis; Hardianto, Ari; Huspa, Desi Harneti Putri; Maharani, Rani; Mayanti, Tri; Darwati, Darwati; Hanafi, Muhammad; Supratman, Unang
ALCHEMY Jurnal Penelitian Kimia Vol 19, No 1 (2023): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.19.1.62384.44-52

Abstract

Kanker payudara merupakan penyebab kedua terbanyak kematian pada wanita akibat kanker setelah kanker paru-paru di seluruh dunia. Steroid merupakan kelompok senyawa aktif yang diantaranya berhasil diisolasi dari genus Chisocheton yang dilaporkan memiliki aktivitas melawan sel kanker payudara MCF-7. Tujuan penelitian ini adalah untuk mengetahui interaksi senyawa steroid terhadap estrogen alfa (ER-α) melalui metode in silico, yaitu penambatan molekul. Pemodelan struktur tiga dimensi (3D) senyawa steroid dilakukan dengan memperhatikan keadaan terprotonasinya pada pH 7,4. Metode in silico divalidasi melalui penambatan ulang struktur kristal ER-α, hingga diperoleh nilai RMSD < 2 Å, dengan program AutoDock 4.2.6. Dengan program yang sama, senyawa-senyawa steroid ditapis dengan metode penambatan molekul. Hasil penapisan menghasilkan nilai energi bebas dari kedua senyawa steroid yaitu -10,08 kkal/mol (7α-hidroksi-β-sitosterol) dan -10,75 kkal/mol (stigmast-5-en-3β-ol), yang nilainya lebih baik dari estradiol (-9,62 kkal/mol), sebagai ligan alami ER-α. Kedua senyawa ini berpotensi untuk menginhibisi estrogen alfa, dimana senyawa stigmast-5-en-3β-ol memiliki potensi yang lebih besar karena nilai energi bebasnya lebih rendah. Hal ini menandakan bahwa modifikasi struktur senyawa mampu mengubah nilai energi ikatan dan interaksi antara ligan dan reseptor.In Silico Study of Steroid Compound Activity Against Breast Cancer Using Estrogen Alpha (ER-α). Breast cancer is the second worldwide leading cause of cancer death in women after lung cancer. Steroids are a group of active compounds isolated from the Chisocheton genus that has activity against MCF-7 breast cancer cells. This study aimed to determine the interaction activity of steroid compounds against alpha estrogen receptor (ER-α) through in silico method specifically mlecular docking. The modeling of the three-dimensional structure (3D) of steroid compounds was performed by considering their protonation states at pH 7.4. The in silico method was validated by redocking the crystal structure of ER-α until obtaining an RMSD value < 2 Å, using the AutoDock 4.2.6 program. Steroids compounds were screened with the same program namely the molecular docking method. Screening results show that the free energy values of the steroid compounds were -10.08 kcal/mol (7α-hydroxy-β-sitosterol) and -10.75 kcal/mol (stigmast-5-en-3β-ol), which are stronger than estradiol (-9.62 kcal/mol) as native ligand of ER-α. Both of these compounds can inhibit the alpha estrogen receptor, in which the stigmast-5-en-3β-ol compound has a greater potential because of its lower free energy value. This finding indicates that modification of the compound's structure could change the binding energy value and interaction between ligands and receptors.
Triterpenoids from The Bark of Garcinia porecta and their Cytotoxic Activity against MCF7 Breast Cancer Lines Darwati Darwati; Alya Tsamrotul; Tati Herlina; Tri Mayanti; Nurlelasari Nurlelasari; Kansy Haikal; Unang Supratman
ALCHEMY Jurnal Penelitian Kimia Vol 15, No 1 (2019): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.15.1.20262.1-9

Abstract

The Garcinia genus is a well known tropical plant in the Indo-Malesiana region and mainly distributed in tropical countries including Indonesia, Thailand, and Malaysia. Previous phytochemical studies on Garcinia species have led to the identification and isolation of mainly prenylated xanthones. This research describes the isolation and structure elucidation of isolated triterpenoids compounds from the bark of Garcinia porecta. Dried powder bark of G. porecta was extracted with methanol and then partitioned with n-hexane, ethyl acetate, and n-butanol. The n-hexane extract then was separated and purified with chromatography techniques to obtain isolated compounds 1 and 2. The chemical structure of isolated compounds was elucidated by spectroscopic methods including one and two-dimensional NMR as well as high-resolution mass spectrometric analysis and identified as lanosterol (1) dan arabidiol (2), respectively. These triterpenoids were isolated from this plant for the first time. Compound 1 and 2 showed weak cytotoxic activity against MCF-7 breast cancer cells with IC50 values of 60.09 dan 46.17 µM, respectively.
A New Limonoid from the Seeds of Chisocheton lasiocarpus (Meliaceae) Ronauli Fitriana; Nurlelasari Nurlelasari; Darwati Darwati; Desi Harneti; Rani Maharani; Tri Mayanti; Unang Supratman
ALCHEMY Jurnal Penelitian Kimia Vol 17, No 2 (2021): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.17.2.44782.219-226

Abstract

 A New Limonoid from the Seeds of Chisocheton lasiocarpus (Meliaceae). Chisocheton is one of Meliaceae genus, which has about 53 species spreading in subtropical and tropical regions. One of the species is Chisocheton lasiocarpus. Chisocheton is rich in limonoids that have various biological activities such as anticancer, antimalarial, anti-inflammatory, antifeedant, antiviral, neuroprotective, and antimicrobial properties. This study aims to isolate limonoids from the seeds of C. lasiocarpus, and determine the structures. The dry powder of seeds of C. lasiocarpus (203.75 g) was macerated with subsequent n-hexane, ethyl acetate, and methanol. Extract of ethyl acetate was separated and purified using chromatography methods until a new limonoid, lasiocarpines (1) and one known limonoid, 14β,15β-epoxynimonol (2) were obtained. The purification process was guided by Ehrlich reagent. The chemical structures were identified by UV, IR, 1H-NMR, 13C-NMR, 2D NMR, and mass spectrometry.
Co-Authors - Ruchiyat . Horizon . Susianti Ace Tatang Hidayat Achmad Zainuddin Achmad Zainuddin Ade Akbar Abdilla Ade Kania Ningsih Ade Kania Ningsih, Ade Kania Adel Zamri Agus Safari Agus Safari Agus Safari Agus Safari Agustini, Dewi Meliati Ahmad Darmawan Ahmad Kurniawan Ahmad Kurniawan Ahmad Ramdan Ahmad Yani Nelly Wahyuni Lia Destiarti Akhmad Darmawan Al Arofatus Naini Al Arofatus Naini Al Arofatus Naini Aldo Hartono Alya Tsamrotul Amir M. Suruwaky Anas Subarnas Anas Urbanas Anastasya Firdausi Andi Rahim Andre A. Sonda Aneu Wahyuni Anjari, Intan Hawina Aprilia Permata Sari Aprilia Permata Sari Aprina, Lutfia Silva Ari Hardianto Ari Widiyantoro Arif Rahman Hakim Arif Rahman Hakim Arif Rahman Hakim Arif Rahman Hakim Arlette Setiawan Arlette Setiawan Arlette Setiawan Arlette Setiawan Arlette Suzy Puspa Pertiwi Arto Yuwono Soeroto Asep Supriadin Asri Peni Wulandari Astrid Feinisa Khairani Aziiz Mardanarian Rosdianto Azmi Azhari Azmi Azhari, Azmi Azmi, Mohamad Nurul Benny Joy Betry Pujiastuti Budiman, Yudha P. C Hanny Wijaya Celcius Waranmaselembun Christina Marpaung Dadan Sumiarsa Danar Dono Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati, Darwati, Darwati Deden Indra Dinata Deden Indra Dinata, Deden Indra Desi Harnet Desi Harneti Desi Harneti Desi Harneti Putri Huspa Dewa G Katja Dewa Gede Katja Dewa Gede Katja Dewa Gede Katja Dikdik Kurnia Dimpuulina Erna Mariati Dini Oktaviani Dondin Sajuthi DUDI RUNADI Edi Suanto, Edi Edi Sukmana Edi Sukmana Edi Sukmana Elis Hastuti Elisabeth Krismayanti Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Erina Hilmayanti Erina Hilmayanti Erina Hilmayanti Erina Hilmayanti Euis Julaeha Euis Julaeha Euis Julaeha Evan Hadrian Ezatul Ezleen Kamarulzaman Fadlilah, Gina Faizah Maira Faizal Hermanto Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar, Mohamad Fajar, Mohamad Fani Rahma Yenita Farabi, Kindi Fathurachman Fauzan Zein Muttaqien Ferdyan Efza Ferry Ferdiansyah Sofian, Ferry Ferdiansyah Filza Yulina Ade Fizrul Indra Lubis Galih Bayu Pratama Galuga Sinalusur Sari Ghina Izdihar Gilang Muhamad Nur Iqbal Gunawan, Latifah Hadi Kuncoro Hadi Kuncoro Hana Goenawan Hanna Goeanawan Hanna Goenawan Harizon Harizon Harlia Harlia Hasbilla, Raihan Fathurrahman Hasnah Osman Hasnah Osman Hedi Paramita Herdanu Rizqullah Hersa Milawati Hersa Milawati Hersa Milawati Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hilmayanti, Erina Huda, Muhammad Badrul Husein Hernadi Bahti Ichsan Nurul Bari Ida Ayu Putu Sri Widnyani Ida Nur Farida Ida Nur Farida Ida Nurfarida Ihsan Rahadian Iis Intan Widiyowati Indri Indriyani Indriyani, Indri Inne Suherna Sasmita Intan Hawina Anjari Intan Rahmayanti Iqbal Musthapa Iqbal Wahyu Mustaqim Isa Mahendra Isramiharti Isramiharti Iwan Setiawan Iwan Setiawan Iwan Setiawan Jamaludin Al-Anshori Jihan Mudrika Rahmi Julia Windi Gunadi Julia Windi Gunadi Julinton Sianturi Julinton Sianturi Kadarusman Kansy Haikal Kansy Haikal Kautsari, Arsi Khadijah Awang Khairunnisa, Shofiyah Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khlaijah Awang Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kok Tong Wong Kristin Shinta Dewi Kusumiyati Laode Rijai Lilis Siti Aisyah Lilis Siti Aisyah Lilis Siti Aisyah Lilis Siti Aisyah, Lilis Siti Lindung Tri Puspasari M. S. Soedjanaatmadja Maira, Faizah Mantiri, Sisilia A Maryati Maryati Mas Rizky A.A. Syamsunarno Max R.J Runtuwene Max R.J Runtuwene Mayshah Purnamasari MEGANTARA, SANDRA Melanie Melanie Milawati, Hersa Moelyono Moektiwardoyo Mohamad Fajar Mohamad Fajar Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi bin Mohamad Taib Mohamad Nurul Azmi Mohamad Taib Mohamad Nurul Azmi Mohamad Taib Mohamed Ashraf Ali Mohd. Zaheen Hassan Mohd. Zaheen Hassan Muchlis, Handi Nugraha Muhamad Nurul Azmi Muhamad Nurul Azmi Muhammad Hanafi Muhammad Hanafi Muhammad Solehin Abd Ghani Muhammad Solehin Abd Ghani Murtihapsari . Mustaqim, Iqbal Wahyu Mustofa, Hidayat Nurul Muttaqin, Fauzan Zein Nadia Mohamed Yusoff Nadya Thufaila Nafiah, Mohamad Azlan Naini, Al Arofatus Nasrudin Nasrudin Nayla Haraswati Noor Rain Abdullah Noor Rain Abdullah Nova Sylviana Nova Sylviana Nunung Kurniasih Nunung Kurniasih Nunung Kurniasih, Nunung Nur Insani Amir Nur Muhammad Miftah Nurabi Ferdiana Nurlelasari Nurlelasari Nurlelasari Okta Wismandanu Paramita, Hedi Ponis Tarigan Prasetyo, Wibowo Budi Pratama, Galih Bayu Prayudi Santoso Primahana, Gian Purbaya, Sari Purnama Purnama Purnama Purnama Purnama Purnama Purwoko, Agus Puspita Sari Putri, Ghesta Alifka Rahmawati Rahmawati Rani Maharani Rani Maharani Rani Maharani Ratu Safitri Ray, Hamidie Ronald Daniel Rejeki, Purwo Sri Revan Hardiawan Richa Mardianingrum Ricson Pemimpin Hutagaol Rika Meliansyah Risyandi Anwar Riza Apriani Rizky Abdulah Rizky Abdullah Romundza, Febbry Ronauli Fitriana Ronny Lesmana Ronny Lesmana Roosje Rosita Oewen, Roosje Rosita Roro Wahyudianingsih Rudi Hendra Rurini Retnowati Rymond Jusuf Rumampuk Safri Ishmayana Safriansyah, Wahyu Salam, Supriatno Salam, Supriyanto Samuel San Parulian Sandra Amalia Riyadi Sari Purbaya Sari Purbaya Sari, Aprilia Permata Satwika Nandiwardhana Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Shabarni Gaffar Shiono, Yoshihito Sianturi, Julinton Sinaga, Siska Elisahbet Sisilia A Mantiri Siska Elisahbet Sinaga Siska Mulya Octavia Siska Mulya Octavia Siti Hani Pratiwi Sofa Fajriah Sofa Fajriah Sofa Fajriah Sofa Fajriah Sofa Fajriah Sofa Fajriah Sofa Fajriah Srikandi, Srikandi Steffi Triany Arnov Subekti Mauluddin Sudarjat Sudarjat Sunarjati Sudigdoadi Supriatno Supriatno Salam Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriyatna Supriyatna Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna, - Suseno Amien Susianti Susianti Susianti Susianti Susianti Susianti, Susianti Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Teddy Budiyansyah Thaigarajan Parumasivam Tiara Prima Amalya Tjandrawati Mozef Toto Subroto Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Reksa Saputra Tri Reksa Saputra Tri Reksa Saputra, Tri Reksa Vicki Nishinarizki Vidia Afina Nuraini Vita Murniati Tarawan Vita Murniati Tarawan W.C. Taylor Wahyu Syafriansyah Wahyuni, Aneu Wawan Hermawan Wawan Hermawan Widyana, Almas Winda Sukmawati Winda Sukmawati Witriany Rayapratiwi Yeni Mulyani Yenny Febriani Yun Yenny Febriani Yun Yenny Febriani Yun, Yenny Febriani Yeong Keng Yoon Yessi Permana Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yum Eryanti Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi, Yuni Susanti Yusup Hidayat