Lucia Tri Suwanti, Lucia Tri
Department Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia. Institute Of Tropical Diseases, Airlangga University, Surabaya, East Java, Indonesia.

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The Potential of Laserpuncture Technology and Probiotics on Weight and Digestibility Crude Protein of Madura Bull at Tlagah Village, Sampang-East Java Muhammad Ahdi Kurniawan; Lucia Tri Suwanti; Mufasirin Mufasirin; R. Tatang Santanu Adikara; Soeharsono Soeharsono; Sunaryo Hadi Warsito; Hana Eliyani; Wahidan Qodiip Maulana
Journal of Applied Veterinary Science And Technology Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I1.2023.5-10

Abstract

Background: Laserpuncture is a technology that uses short-wave rays to receptors (acupuncture points) to increase the capacity and efficiency of organs to increase the productivity of animals’ livestock. This can improve feed efficiency to help breeders maximize their beef bull fattening business. Purpose: This study was to test the potential of laserpuncture and probiotics on body weight gain and the digestibility of crude protein in Madura bull. Methods: This study used 12 Madura bulls with an age range of 2-2.5 years. The bulls were divided into two treatment groups. The P1 group shot laserpuncture at a dose of 0.5 joules. The P2 group received laserpuncture at a dose of 0.5 joules with 5 ml of probiotics administration in 1 liter of water. Data was taken by recording the weight gain of Madurese bulls every week and the results of crude protein digestibility testing. The resulting data is then analyzed using the T-test. Results: This study showed no significant difference between P1 and P2 in male Madura cattle’s body weight gain and crude protein digestibility. Conclusion: The combination of laserpuncture at the growth point and probiotic administration in feed did not cause a significant difference in weight gain or digestibility of crude protein in male Madurese bull.
PENINGKATAN TNF-α DAN INDEKS APOPTOSIS PADA TULANG MENCIT YANG DIINFEKSI Toxoplasma gondii Lucia Tri Suwanti; Mufasirin M
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.115 KB) | DOI: 10.24815/jn.v%vi%i.2808

Abstract

Penelitian ini bertujuan mengetahui peningkatan tumor necrosis factor-alpha (TNF-α) dan indeks apoptosis pada tulang mencit yang  diinfeksi Toxoplasma gondii (T. gondii). Tiga puluh dua ekor mencit dibagi menjadi dua kelompok. Kelompok 1 (K1), merupakan kelompok kontrol, tidak diinfeksi sedangkan Kelompok 2 (K2) diinfeksi dengan 10 takizoit T. gondii secara intraperitoneal. Enam hari setelah infeksi mencit dikorbankan, diambil tulang femur dan dilakukan pembuatan preparat histologis dengan pengecatan immunohistochemistry (IHC) dan Tunel assay. Hasil penelitian menunjukkan jumlah sel tulang yang mengekspresikan TNF-α pada K2 (27,04±6,92) berbeda sangat nyata dibandingkan dengan K1 (11,42±3,92). Indeks apoptosis pada K1 dan K2 masing-masing adalah 9,17±3,04 dan 16,28±3,37. Dari hasil penelitian disimpulkan bahwa infeksi T. gondii meningkatkan TNF-α dan indeks apoptosis sel tulang femur.
THE DILATATION OF BRAIN VENTRICLE DUE TO CONGENITAL TOXOPLASMOSIS IN MICE CORRELATED WITH APOPTOSIS BUT NOT WITH TRANSFORMING GROWTH FACTOR BETA Lucia Tri Suwanti; Mufasirin Mufasirin; Hani Plumeriastuti
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.441 KB) | DOI: 10.21157/j.ked.hewan.v12i1.5428

Abstract

This study aimed to determine the occurences of mice brain ventricles dilatation that congenitally infected with Toxoplasma gondii (T. gondii) as a marker of hydrocephalus and cellular changes in the brain. A total of twenty pregnant mice (11.5 days pregnacy) were divided into 2 groups, which were control (P1) group and treatment (P2) group. The mice in the treatment group were infected with 101 tachyzoites of T. gondii. All mice were maintained until delivery. The newborn mice were sacrificed and their brain were removed and fixed in 10% buffered formalin to prepare histology slides with HE staining for observation of ventricular width, TUNEL assay for apoptosis observation, and immunohistochemistry for the expression of transforming growth factor beta (TGF-β) observations. The data were analyzed using t test and linear regression. The results showed that ventricular width and apoptosis index significantly increased (P0.01) in the treatment group compared to control group, but there was no difference in the expression of TGF-β (P0.05) in both groups. Dilatation of ventricle correlated with the apoptotic index of brain cells but did not correlated with the expression of TGF-β.
PENINGKATAN TNF-α DAN INDEKS APOPTOSIS PADA TULANG MENCIT YANG DIINFEKSI Toxoplasma gondii Lucia Tri Suwanti; Mufasirin M
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2808

Abstract

Penelitian ini bertujuan mengetahui peningkatan tumor necrosis factor-alpha (TNF-α) dan indeks apoptosis pada tulang mencit yang  diinfeksi Toxoplasma gondii (T. gondii). Tiga puluh dua ekor mencit dibagi menjadi dua kelompok. Kelompok 1 (K1), merupakan kelompok kontrol, tidak diinfeksi sedangkan Kelompok 2 (K2) diinfeksi dengan 10 takizoit T. gondii secara intraperitoneal. Enam hari setelah infeksi mencit dikorbankan, diambil tulang femur dan dilakukan pembuatan preparat histologis dengan pengecatan immunohistochemistry (IHC) dan Tunel assay. Hasil penelitian menunjukkan jumlah sel tulang yang mengekspresikan TNF-α pada K2 (27,04±6,92) berbeda sangat nyata dibandingkan dengan K1 (11,42±3,92). Indeks apoptosis pada K1 dan K2 masing-masing adalah 9,17±3,04 dan 16,28±3,37. Dari hasil penelitian disimpulkan bahwa infeksi T. gondii meningkatkan TNF-α dan indeks apoptosis sel tulang femur.
THE DILATATION OF BRAIN VENTRICLE DUE TO CONGENITAL TOXOPLASMOSIS IN MICE CORRELATED WITH APOPTOSIS BUT NOT WITH TRANSFORMING GROWTH FACTOR BETA Lucia Tri Suwanti; Mufasirin Mufasirin; Hani Plumeriastuti
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.5428

Abstract

This study aimed to determine the occurences of mice brain ventricles dilatation that congenitally infected with Toxoplasma gondii (T. gondii) as a marker of hydrocephalus and cellular changes in the brain. A total of twenty pregnant mice (11.5 days pregnacy) were divided into 2 groups, which were control (P1) group and treatment (P2) group. The mice in the treatment group were infected with 101 tachyzoites of T. gondii. All mice were maintained until delivery. The newborn mice were sacrificed and their brain were removed and fixed in 10% buffered formalin to prepare histology slides with HE staining for observation of ventricular width, TUNEL assay for apoptosis observation, and immunohistochemistry for the expression of transforming growth factor beta (TGF-β) observations. The data were analyzed using t test and linear regression. The results showed that ventricular width and apoptosis index significantly increased (P0.01) in the treatment group compared to control group, but there was no difference in the expression of TGF-β (P0.05) in both groups. Dilatation of ventricle correlated with the apoptotic index of brain cells but did not correlated with the expression of TGF-β.
Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Muhammad Rofi' Prasetya; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Kusnoto Kusnoto; Muchammad Yunus
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18201

Abstract

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.
Efektivitas Vaksinasi Crude dan Soluble Protein Spora Myxobolus Koi terhadap Tingkat Kerusakan Usus Ikan Koi (Cyprinus carpio Koi) [ The Effectivety Crude and Soluble Protein of Myxobolus Koi Spore againts Intestine Different Degrees in Koi (Cyprinus carpio Koi)] Gunanti Mahasri; Rachma Woro Anggarani; Lucia Tri Suwanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i1.11233

Abstract

Abstract Myxobolus koi is one species of Myxobolus sp that can cause parasitic diseases in fish called Myxobolusis. Based on the Decree of the Minister of Marine and Fisheries No: KEP.03/MEN/2010 that Myxobolus koi in the list of Fish Quarantine Pests group I. Myxosporean diseases are most numerous in the water can cause Proliferative Kidney Disease (PKD) and Whirling Disease (WD). The aim of this research is to finding, analyzing and determining the protein of spores Myxobolus koi that can effectively reduce the level of damage to the intestinal organs as well as for the prevention myxobolusis on Koi's. Then for finding, analyzing and determining the protein of spores Myxobolus koi do isolation of spore proteins. The study consisted of three phases examination to preparation and identification of spores, isolation and analyze of crude and soluble protein spores for obtain dose and molecular weight each protein and histopathological test. This research uses descriptive method. The data presented may be narratives, images, tables or charts for each group. Intestinal histopathology test results of research carried scoring Koi's were analyzed using the Kruskal-Wallis. The results showed a profile crude protein and soluble proteins from spores Myxobolus koi showed that the molecular weight of crude protein Myxobolus koi in this study was 150 kDa and 72 kDa and for soluble protein was 73 kDa. Results scoring the degree of infection caused by exposure to Myxobolus koi then statistically processed with an average yield of scoring in a sequence of 0; 1.6; 0.64 and 0.32. Statistical analysis showed no significant difference in the treatment of K + with crude protein, and K + with soluble proteins. Statistical analysis showed that there were significant differences in treatment with K+ and K-, K- with soluble protein and crude protein and soluble protein. Histopathological changes in the intestine in the form of inflammatory cell infiltration, necrosis and haemorage
The Activity of Mixed Microalgae Polysaccharides from Indonesia as Anti-Malaria in Vitro Mahendra Pujiyanto; Zhaza Afililla; Lilik Maslachah; Thomas Valentinus Widiyatno; Mochamad Donny Koerniawan; Eko Agus Suyono; Arief Budiman; Ulfah Juniarti Siregar; Lucia Tri Suwanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.34766

Abstract

Highlight Research One of the content of microalgae that is beneficial for health is polysaccharides Polysaccharides of Indonesian microalgae can be promoted as anti-malarial Polysaccharides from Glagah, Spirulina and East Java microalgae inhibited the growth of plasmodium in vitro and had IC50 values of 3.18 µg/mL, 5.43µg/mL and 9.87 µg/mL, respectively   Abstract Malaria is an infectious disease caused by protozoan parasites of the genus Plasmodium that categorized as deadliest diseases in the world. Artemisinin and its derivatives are still recommended drugs for malaria therapy, however, there have been indications that Plasmodium parasites are resistant to this drug. Therefore, a study on polysaccharides from microalgae may be a potential as bioactive compound for anti-malaria. The aim of this study was to determine the effectiveness of the mixed microalgae polysaccharides as anti-malarial in vitro. Polysaccharides were extracted from three microalgae Spirulina sp., mixed microalgae Glagah and mixed microalgae East Java using the alkaline extraction method. The anti-malarial activity test refers to the concentration of polysaccharides used in calculating the IC50 value by probit analysis.  The concentration of polysaccharides of the three microalgae used were 0; 0.01; 0.01, 1, 10 and 100 µg/mL. The results showed that the IC50 values of polysaccharides of Glagah, Spirulina sp. and East Java microalgae were 3.18 µg/mL, 5.43µg/mL, and 9.87 µg/mL, respectively. In Conclusion, polysaccharides of Indonesian mixed microalgae can be promoted as anti-malarial.
Epidemiological Studies of Subclinical Mastitis in Dairy Goats in Lumajang Regency, East Java, Indonesia Mira Fatmawati Fatmawati; Lucia Tri Suwanti; Mufasirin; Mirni Lamid; Nunuk Dyah Retno Lastuti; Endang Suprihati; Mustofa Helmi Effendi; Anam Al Arif; Widi Nugroho; Masdiana C Padaga
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 3 (2023): December 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.03.07

Abstract

Subclinical mastitis is an economically important disease in dairy goat farming. Lumajang is a center for dairy goats in East Java. However, routine examination of subclinical mastitis for screening tests has no data. This study aimed to provide prevalence data and identification factors that influenced subclinical mastitis. Therefore, this study was carried out as part of the national surveillance program. The research design is a cross-sectional study with simple random sampling. Samples came from 19 farms with a total individual sample of 224 dairy goats. Detection of subclinical mastitis using an undirect test with the California Mastitis Test (CMT). Identification of management factors and characteristics that can influence subclinical mastitis in dairy goat farms using a structured questionnaire. The results showed that the prevalence of subclinical mastitis in Lumajang District was 36.2%. There was a significant difference in subclinical mastitis in each village (p<0.05). The highest prevalence of subclinical mastitis was in Kandangtepus village. A farmer's characteristic factor that significantly influences subclinical mastitis is the farmer's experience. The aspect of livestock management that significantly affects subclinical mastitis is structured work management. Subclinical mastitis was prevalent in dairy goats in Lumajang District and requires prevention efforts. Prevention approach through education for farmers to increase knowledge, attitude, and socialization to improve livestock management.
Identification, Prevalence, and Degree of Digestive Tract Protozoa Infection in Dairy Cows at KUTT Suka Makmur Grati Pasuruan Margaretha, Josephira Intan; Hastutiek, Poedji; Permatasari, Dian Ayu; Sunarso, Agus; Rahardjo, Dadik; Suwanti, Lucia Tri
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60454

Abstract

The aim of this research is to identify, determine prevalence, and degree infection of protozoa gastrointestinal tract in dairy cow at KUTT Suka Makmur, Pasuruan district. The research was conducted from July until September 2023 by taking 100 dairy cow faeces sample from 4 villages of KUTT Suka Makmur. The samples were examined by sedimentation and floating methods. Species are identified by looking at morphology of protozoa gastrointestinal. There were of 85 (85%) samples positively infected with protozoan. There were 3 species that infect the digestive tract, those are Eimeria sp., Balantidium sp., and Blastocystis sp. The highest infection of protozoa in this research was Balantidium sp. (54%) followed by Eimeria sp. (47%). In this study, we found that 54 samples (54%) infected by one kind of protozoa and 31 samples (31%) infected by mixed protozoa. The result of Chi Square showed no significant difference in the age on the prevalence of digestive tract protozoa. The result of Kruskal Wallis showed no significant difference in the age and degree infection of Eimeria sp.
Co-Authors A'la, Rofiqul Abdul Samik Adi Sofyan Ansori, Muhammad Adikara, Tatang Santanu Agustina, Firanda Akbar Wijaya Putra Purnama Al Arif, M Anam Alasrorik, Muhammad Hizbulloh Alfina Azkiana Amara Lintang Pagati Amirotul Azhimah Ana Amaliah Anam Al Arif Andi Jayawardhana andi jayawardhana Anton Sulistiono Arief Budiman Arief Budiman Arif Pratiwi Arimbi Arimbi Aswin, Ahmad Azizah Bilqis Nurkarimah Bambang Sektiari Lukiswanto Bani Bacan Hacantya Yudanagara Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan Bryan Ahmad Affan Lubis Budiarto Budiarto Cyuzuzo Callixte Dadik Rahardjo, Dadik Desiandura, Kurnia Dewi Purwatiningsih Dewi, Aprilia Kurnia Didik Handijatno Didik Handijatno Dinda Eka Putri Diyantoro Diyantoro, Diyantoro Djoko Legowo Doohan Mahendra Dwi Ananda, Novita DWI PUTRI RAHMAWATI Dyah Ayu Kurniawati Dyah Ayu Kurniawati Eduardus Bimo Aksono Herupradoto Eka Noviya Fuzianingsih Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah Eka Pramyrtha Hestinah Eko Agus Suyono Eko Agus Suyono Eldatia Utari Putri Elok Apriliawati Endang Retno Surjaningrum Endang Suprihati Endang Suprihati Erma Safitri Esti Yunitasari Estoepangestie, Agnes Theresia Soelih Fadlilah, Shinta Levea Ni'matul Fania Selfiannisa Fauziah Fitri Hernanto Febrina Dian Permatasari Fedik Abdul Rantam Felita Widyaningsih Fransiska Cicilia Beka Galaxy Guardian Gandul Atik Yuliani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hanna Harnida, Hanna Harijani, Nenny Helen Susilowati, Helen Hemasayu Nirmala Putri Heni Puspitasari Heny Arwati Hernanda, Ary Setya Herry Agoes Hermadi Hidajanti, Nove Ida Yuniarti Idha Kusumawati Imam Mustofa Indah Norma Triana Indasari, Elly Nur Ine Karni Ira Sari Yudaniayanti Jola Rahmahani Kadek Rachmawati Khairullah, Aswin Rafif Koesdarto Koesdarto Koesnoto Supriandono Kuncorojakti, Suryo Kurniawan, Muhammad 'Ahdi Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lantip Muhammad Dewabrata Lilik Maslachah Lutfi Agus Salim M Iqbal Maulana Ginting Mafruchati, Maslichah Mahendra Pujiyanto Margaretha, Josephira Intan Marselaonety La’lang Masdiana C Padaga Melani Anggraini Meles, Dewa Ketut Meta Aprilia Mira Fatmawati Fatmawati Mirni Lamid Mochamad Donny Koerniawan Mochammad Donny Koerniawan Mohammad Sukmanadi Moses, Ikechukwu Benjamin Muchammad Yunus Mufa, Ramy Inas Mahirah Mufasirin Muhammad Ahdi Kurniawan Muhammad Ridwan Muhammad Rofi&#039; Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Mustofa Helmi Effendi Ngakan Made Rai Widjaja Ni Komang Aprilina Widisuputri Nidom, Chairul Anwar Niluh Selly Frantika Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nur Ainy Fardana N. Nurdianto, Arif Rahman Nursalim, Muhammad Tauhid Nusdianto Triakoso Paraswita Eindah Fitri Permatasari, Dian Ayu Poedji Hastutiek Prasetya, Muhammad Rofi' Pratama, Bima Putra Pratama, Ponasari Galuh Pratiwi, Arif Primarizky, Hardany Puput, Sesa Purnamasari, Kartika Rachma Woro Anggarani Rahadju Ernawati Rahmahani, Jola Ramandinianto, Sancaka Chasyer Ratna Damayanti Retno Yuli Rimayanti Rimayanti Rina Vitriasari Riwu, Katty Hendriana Priscilia Rochmah Kurnijasanti Romy Muhammad Dary Mufa Santoso, Kuncoro Puguh Saputra, Rakan Mahiid Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawan Koesdarto Setiawan Kusdarto Soeharsono Soeharsono Sri Chusniati Sri Mulyati Sri Mumpuni Sri Subekti Suherni Susilowati Suherni Susilowati Sunarso, Agus Supriyadi Suzanita Utama Tatik Hernawati Taufik Tri Laksono Tita Damayanti Lestari Tony Hartono Tri Wahyu Suprayogi Tyasningsih, Wiwiek Tyasningsih, Wiwiek Ulfah Juniarti Siregar Utami, Kinanti Putri Vanna Lidya Kharisma Vindo Rossy Pertiwi Wahidan Qodiip Maulana Wahyudi, Nurul Tri Warda Nafalizza Efendi Warsito, Sunaryo Hadi Widi Nugroho Widisuputri, Ni Komang Aprilina Widiyatno, Thomas Valentinus Widya Paramita Lokapirnasari Wijaya, Andi Yasmin Wisnu Nurcahyo Wiwik Misaco Yuniarti Wurlina, W Yonatan Dimascahyo Budianto Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla