Lucia Tri Suwanti, Lucia Tri
Department Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia. Institute Of Tropical Diseases, Airlangga University, Surabaya, East Java, Indonesia.

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Peta Potensi Pengentasan Stunting di Kota Surabaya Endang Retno Surjaningrum; Eldatia Utari Putri; Nur Ainy Fardana N.; Lucia Tri Suwanti; Lutfi Agus Salim; Esti Yunitasari; Bani Bacan Hacantya Yudanagara; Lantip Muhammad Dewabrata
Media Gizi Indonesia Vol. 17 No. 1SP (2022): Media Gizi Indonesia (National Nutrition Journal) Special Issue: Internation
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mgi.v17i1SP.97-103

Abstract

Penelitian ini bertujuan menunjukkan faktor protektif atau pendukung, dan faktor resiko atau penghambat program penanganan stunting yang ditemui di kelurahan lokus stunting dengan kasus stunting terbanyak di Kota Surabaya. Penduduk kota Surabaya memiliki latar belakang sosial-budaya dan ekonomi serta lingkungan yang beragam. Penelitian dilakukan menggunakan Focus Group Discussion yang dilaksanakan bersama tim kelurahan yang terdiri dari Kader, PLKB, PKK, tim Rumah Dataku, guru PAUD, serta TPK di sepuluh (10) kelurahan di bulan September-Oktober 2022 menggunakan panduan pertanyaan yang disusun oleh tim peneliti. Data dianalisis secara deskriptif dengan kategori faktor protektif dan faktor resiko. Hasil data menggambarkan keragaman situasi di masing-masing kelurahan. Hasil akan dikombinasikan dengan hasil data audit stunting sebagai rekomendasi strategi penanganan stunting kepada Dinas terkait di Surabaya.
Inability of Polysaccharides of Spirulina Platensis to Protect Hepatocyte Cells Line on Toxoplasma Gondii Infection In Vitro Lucia Tri Suwanti
Journal of Global Pharma Technology Volume 12 Issue 01
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Toxoplasmosis is one zoonotic disease which caused by an obligate intracellular protrozoan parasite,  Toxoplasma gondii. The parasite infects humans and warm-blooded animals (mammals and birds). The drug of choice against toxoplasmosis is combination between pyrimethamin with sulfadiazine, but both can induce side effects and toxic to host cell, so alternative therapeutic compounds are needed.  Many reseachers have explored microalgae for food and medicine. One of the main components of  Spirulina platensis that plays a role in enhanching immunity is polysaccharides. The present study investigated effect of Spirulina polysaccharides on Toxoplasma gondii infection in Vitro. Polysaccharides of S. platensis were extracted with hot water and alkhaline method. Extraction Rate (ER), sugar and protein levels were compared between the two methods. The Hepatocyte Cells Line (HCL) were infected with T. gondii and treated with polysaccharides of S. platensis. The parameters were Parasite burden and Viability Host Cell. The result shows that Extraction Rate, carbohydrate and protein levels were affected by extraction method. Carbohydrate level in polysaccharides with hot water extraction was higher than alkhaline extraction, on the contrary, ER and protein level were lower. Polysaccharides of S. platensis did not protect HCL infected with T. gondii. More than 5 mg/mL polysaccharides of S. platensis was toxic to host HCL.  Keywords: Alkaline extraction, Hot water extraction, Polysaccharides, Spirulina platensis, Toxoplasma gondii.
The Potential of Laserpuncture Technology and Probiotics on Weight and Digestibility Crude Protein of Madura Bull at Tlagah Village, Sampang-East Java Muhammad Ahdi Kurniawan; Lucia Tri Suwanti; Mufasirin Mufasirin; R. Tatang Santanu Adikara; Soeharsono Soeharsono; Sunaryo Hadi Warsito; Hana Eliyani; Wahidan Qodiip Maulana
Journal of Applied Veterinary Science And Technology Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I1.2023.5-10

Abstract

Background: Laserpuncture is a technology that uses short-wave rays to receptors (acupuncture points) to increase the capacity and efficiency of organs to increase the productivity of animals’ livestock. This can improve feed efficiency to help breeders maximize their beef bull fattening business. Purpose: This study was to test the potential of laserpuncture and probiotics on body weight gain and the digestibility of crude protein in Madura bull. Methods: This study used 12 Madura bulls with an age range of 2-2.5 years. The bulls were divided into two treatment groups. The P1 group shot laserpuncture at a dose of 0.5 joules. The P2 group received laserpuncture at a dose of 0.5 joules with 5 ml of probiotics administration in 1 liter of water. Data was taken by recording the weight gain of Madurese bulls every week and the results of crude protein digestibility testing. The resulting data is then analyzed using the T-test. Results: This study showed no significant difference between P1 and P2 in male Madura cattle’s body weight gain and crude protein digestibility. Conclusion: The combination of laserpuncture at the growth point and probiotic administration in feed did not cause a significant difference in weight gain or digestibility of crude protein in male Madurese bull.
PENINGKATAN TNF-α DAN INDEKS APOPTOSIS PADA TULANG MENCIT YANG DIINFEKSI Toxoplasma gondii Lucia Tri Suwanti; Mufasirin M
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.115 KB) | DOI: 10.24815/jn.v%vi%i.2808

Abstract

Penelitian ini bertujuan mengetahui peningkatan tumor necrosis factor-alpha (TNF-α) dan indeks apoptosis pada tulang mencit yang  diinfeksi Toxoplasma gondii (T. gondii). Tiga puluh dua ekor mencit dibagi menjadi dua kelompok. Kelompok 1 (K1), merupakan kelompok kontrol, tidak diinfeksi sedangkan Kelompok 2 (K2) diinfeksi dengan 10 takizoit T. gondii secara intraperitoneal. Enam hari setelah infeksi mencit dikorbankan, diambil tulang femur dan dilakukan pembuatan preparat histologis dengan pengecatan immunohistochemistry (IHC) dan Tunel assay. Hasil penelitian menunjukkan jumlah sel tulang yang mengekspresikan TNF-α pada K2 (27,04±6,92) berbeda sangat nyata dibandingkan dengan K1 (11,42±3,92). Indeks apoptosis pada K1 dan K2 masing-masing adalah 9,17±3,04 dan 16,28±3,37. Dari hasil penelitian disimpulkan bahwa infeksi T. gondii meningkatkan TNF-α dan indeks apoptosis sel tulang femur.
THE DILATATION OF BRAIN VENTRICLE DUE TO CONGENITAL TOXOPLASMOSIS IN MICE CORRELATED WITH APOPTOSIS BUT NOT WITH TRANSFORMING GROWTH FACTOR BETA Lucia Tri Suwanti; Mufasirin Mufasirin; Hani Plumeriastuti
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.441 KB) | DOI: 10.21157/j.ked.hewan.v12i1.5428

Abstract

This study aimed to determine the occurences of mice brain ventricles dilatation that congenitally infected with Toxoplasma gondii (T. gondii) as a marker of hydrocephalus and cellular changes in the brain. A total of twenty pregnant mice (11.5 days pregnacy) were divided into 2 groups, which were control (P1) group and treatment (P2) group. The mice in the treatment group were infected with 101 tachyzoites of T. gondii. All mice were maintained until delivery. The newborn mice were sacrificed and their brain were removed and fixed in 10% buffered formalin to prepare histology slides with HE staining for observation of ventricular width, TUNEL assay for apoptosis observation, and immunohistochemistry for the expression of transforming growth factor beta (TGF-β) observations. The data were analyzed using t test and linear regression. The results showed that ventricular width and apoptosis index significantly increased (P0.01) in the treatment group compared to control group, but there was no difference in the expression of TGF-β (P0.05) in both groups. Dilatation of ventricle correlated with the apoptotic index of brain cells but did not correlated with the expression of TGF-β.
Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Muhammad Rofi' Prasetya; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Kusnoto Kusnoto; Muchammad Yunus
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18201

Abstract

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.
Efektivitas Vaksinasi Crude dan Soluble Protein Spora Myxobolus Koi terhadap Tingkat Kerusakan Usus Ikan Koi (Cyprinus carpio Koi) [ The Effectivety Crude and Soluble Protein of Myxobolus Koi Spore againts Intestine Different Degrees in Koi (Cyprinus carpio Koi)] Gunanti Mahasri; Rachma Woro Anggarani; Lucia Tri Suwanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i1.11233

Abstract

Abstract Myxobolus koi is one species of Myxobolus sp that can cause parasitic diseases in fish called Myxobolusis. Based on the Decree of the Minister of Marine and Fisheries No: KEP.03/MEN/2010 that Myxobolus koi in the list of Fish Quarantine Pests group I. Myxosporean diseases are most numerous in the water can cause Proliferative Kidney Disease (PKD) and Whirling Disease (WD). The aim of this research is to finding, analyzing and determining the protein of spores Myxobolus koi that can effectively reduce the level of damage to the intestinal organs as well as for the prevention myxobolusis on Koi's. Then for finding, analyzing and determining the protein of spores Myxobolus koi do isolation of spore proteins. The study consisted of three phases examination to preparation and identification of spores, isolation and analyze of crude and soluble protein spores for obtain dose and molecular weight each protein and histopathological test. This research uses descriptive method. The data presented may be narratives, images, tables or charts for each group. Intestinal histopathology test results of research carried scoring Koi's were analyzed using the Kruskal-Wallis. The results showed a profile crude protein and soluble proteins from spores Myxobolus koi showed that the molecular weight of crude protein Myxobolus koi in this study was 150 kDa and 72 kDa and for soluble protein was 73 kDa. Results scoring the degree of infection caused by exposure to Myxobolus koi then statistically processed with an average yield of scoring in a sequence of 0; 1.6; 0.64 and 0.32. Statistical analysis showed no significant difference in the treatment of K + with crude protein, and K + with soluble proteins. Statistical analysis showed that there were significant differences in treatment with K+ and K-, K- with soluble protein and crude protein and soluble protein. Histopathological changes in the intestine in the form of inflammatory cell infiltration, necrosis and haemorage
The Activity of Mixed Microalgae Polysaccharides from Indonesia as Anti-Malaria in Vitro Mahendra Pujiyanto; Zhaza Afililla; Lilik Maslachah; Thomas Valentinus Widiyatno; Mochamad Donny Koerniawan; Eko Agus Suyono; Arief Budiman; Ulfah Juniarti Siregar; Lucia Tri Suwanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.34766

Abstract

Highlight Research One of the content of microalgae that is beneficial for health is polysaccharides Polysaccharides of Indonesian microalgae can be promoted as anti-malarial Polysaccharides from Glagah, Spirulina and East Java microalgae inhibited the growth of plasmodium in vitro and had IC50 values of 3.18 µg/mL, 5.43µg/mL and 9.87 µg/mL, respectively   Abstract Malaria is an infectious disease caused by protozoan parasites of the genus Plasmodium that categorized as deadliest diseases in the world. Artemisinin and its derivatives are still recommended drugs for malaria therapy, however, there have been indications that Plasmodium parasites are resistant to this drug. Therefore, a study on polysaccharides from microalgae may be a potential as bioactive compound for anti-malaria. The aim of this study was to determine the effectiveness of the mixed microalgae polysaccharides as anti-malarial in vitro. Polysaccharides were extracted from three microalgae Spirulina sp., mixed microalgae Glagah and mixed microalgae East Java using the alkaline extraction method. The anti-malarial activity test refers to the concentration of polysaccharides used in calculating the IC50 value by probit analysis.  The concentration of polysaccharides of the three microalgae used were 0; 0.01; 0.01, 1, 10 and 100 µg/mL. The results showed that the IC50 values of polysaccharides of Glagah, Spirulina sp. and East Java microalgae were 3.18 µg/mL, 5.43µg/mL, and 9.87 µg/mL, respectively. In Conclusion, polysaccharides of Indonesian mixed microalgae can be promoted as anti-malarial.
Identification, Prevalence, and Degree of Digestive Tract Protozoa Infection in Dairy Cows at KUTT Suka Makmur Grati Pasuruan Margaretha, Josephira Intan; Hastutiek, Poedji; Permatasari, Dian Ayu; Sunarso, Agus; Rahardjo, Dadik; Suwanti, Lucia Tri
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60454

Abstract

The aim of this research is to identify, determine prevalence, and degree infection of protozoa gastrointestinal tract in dairy cow at KUTT Suka Makmur, Pasuruan district. The research was conducted from July until September 2023 by taking 100 dairy cow faeces sample from 4 villages of KUTT Suka Makmur. The samples were examined by sedimentation and floating methods. Species are identified by looking at morphology of protozoa gastrointestinal. There were of 85 (85%) samples positively infected with protozoan. There were 3 species that infect the digestive tract, those are Eimeria sp., Balantidium sp., and Blastocystis sp. The highest infection of protozoa in this research was Balantidium sp. (54%) followed by Eimeria sp. (47%). In this study, we found that 54 samples (54%) infected by one kind of protozoa and 31 samples (31%) infected by mixed protozoa. The result of Chi Square showed no significant difference in the age on the prevalence of digestive tract protozoa. The result of Kruskal Wallis showed no significant difference in the age and degree infection of Eimeria sp.
Prevalence and Infection’s Degree of Gastrointestinal Nematode Worm in Friesian Holstein Dairy Cows at KUTT Suka Makmur Pasuruan Regency Utami, Kinanti Putri; Santoso, Kuncoro Puguh; Suwanti, Lucia Tri; Hastutiek, Poedji; Permatasari, Dian Ayu; Sunarso, Agus
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60504

Abstract

This study aims to determine the type, prevalence, and infection’s degree of gastrointestinal Nematode worm in Friesian Holstein Dairy Cows at KUTT Suka Makmur, Pasuruan Regency. The study was conducted in July – September 2023. One hundred of feces samples were collected from four districts at KUTT Suka Makmur working area. Samples were examined by sedimentation and floating methods. Positive samples were further examined by McMaster methods to determine the number of worm eggs per gram feces. The type of worms were Oesophagostomum sp. (24%), Haemonchus sp. (20%), Mecistocirrus sp. (13%), Trichuris sp. (8%), Ostertagia sp. (6%), Trichostrongylus sp. (6%), Nematodirus sp. (3%), and Strongyloides sp. (3%). The prevalence of gastrointestinal Nematode worm was 66% with mild and severe degrees of infection. The Chi Square test results showed that age had no effect on the prevalence and infection’s degree of gastrointestinal Nematode worm in Friesian Holstein dairy cows at KUTT Suka Makmur. While location had effect on the prevalence but had no effect on the infection’s degree.
Co-Authors A'la, Rofiqul Abdul Samik Adi Sofyan Ansori, Muhammad Adikara, Tatang Santanu Agustina, Firanda Akbar Wijaya Putra Purnama Aksono HP., Eduardus Bimo Al Arif, M Anam Alasrorik, Muhammad Hizbulloh Alfina Azkiana Amara Lintang Pagati Amirotul Azhimah Ana Amaliah Anam Al Arif Andi Jayawardhana andi jayawardhana Anton Sulistiono Arief Budiman Arief Budiman Arif Pratiwi Arimbi Arimbi Aswin, Ahmad Azizah Bilqis Nurkarimah Bambang Sektiari Lukiswanto Bani Bacan Hacantya Yudanagara Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan Bryan Ahmad Affan Lubis Budiarto Budiarto Budiarto Choirunnisa, Indaka Rachmah Chusniati, Sri Cyuzuzo Callixte Dadik Rahardjo, Dadik Desiandura, Kurnia Dewi Purwatiningsih Dewi, Aprilia Kurnia Didik Handijatno Didik Handijatno Dinda Eka Putri Diyantoro Diyantoro, Diyantoro Djoko Legowo Doohan Mahendra Dwi Ananda, Novita DWI PUTRI RAHMAWATI Dyah Ayu Kurniawati Dyah Ayu Kurniawati Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah Eka Pramyrtha Hestinah Eko Agus Suyono Eko Agus Suyono Eldatia Utari Putri Elok Apriliawati Endang Retno Surjaningrum Endang Suprihati Erma Safitri Esti Yunitasari Estoepangestie, Agnes Theresia Soelih Fadlilah, Shinta Levea Ni'matul Fania Selfiannisa Fatmawati, Mira Fatmawati Fauziah Fitri Hernanto Febrina Dian Permatasari Fedik Abdul Rantam Felita Widyaningsih Fitri, Paraswita Eindah Fransiska Cicilia Beka Fuzianingsih, Eka Noviya Galaxy Guardian Gandul Atik Yuliani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hanna Harnida, Hanna Harijani, Nenny Helen Susilowati, Helen Hemasayu Nirmala Putri Heni Puspitasari Heny Arwati Hernanda, Ary Setya Herry Agoes Hermadi Hidajanti, Nove Ida Yuniarti Idha Kusumawati Imam Mustofa Indah Norma Triana, Indah Norma Indasari, Elly Nur Ine Karni Ira Sari Yudaniayanti Kadek Rachmawati Khairullah, Aswin Rafif Koesdarto Koesdarto Koesnoto Supriandono Kuncorojakti, Suryo Kurniawan, Muhammad 'Ahdi Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lantip Muhammad Dewabrata Lilik Maslachah Lutfi Agus Salim M Iqbal Maulana Ginting Mafruchati, Maslichah Mahendra Pujiyanto Margaretha, Josephira Intan Marselaonety La’lang Masdiana C Padaga Melani Anggraini Meles, Dewa Ketut Meta Aprilia Mirni Lamid Mochamad Donny Koerniawan Mochammad Donny Koerniawan Mohammad Sukmanadi Moses, Ikechukwu Benjamin Muchammad Yunus Mufa, Ramy Inas Mahirah Mufasirin Muhammad Ahdi Kurniawan Muhammad Ridwan Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Ngakan Made Rai Widjaja Ni Komang Aprilina Widisuputri Nidom, Chairul Anwar Niluh Selly Frantika Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nur Ainy Fardana N. Nurdianto, Arif Rahman Nursalim, Muhammad Tauhid Nusdianto Triakoso Permatasari, Dian Ayu Poedji Hastutiek Prasetya, Muhammad Rofi' Pratama, Bima Putra Pratama, Ponasari Galuh Pratiwi, Arif Primarizky, Hardany Puput, Sesa Purnamasari, Kartika Rachma Woro Anggarani Rahadju Ernawati Raharjo, Dadik Rahmahani, Jola Ramandinianto, Sancaka Chasyer Ratna Damayanti Retno Yuli Rimayanti Rimayanti Rina Vitriasari Riwu, Katty Hendriana Priscilia Rochmah Kurnijasanti Romy Muhammad Dary Mufa Santoso, Kuncoro Puguh Saputra, Rakan Mahiid Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawan Koesdarto Setiawan Kusdarto Soeharsono Soeharsono Sri Mulyati Sri Mumpuni Sri Subekti Suherni Susilowati Sunarso, Agus Supriyadi Suzanita Utama Tatik Hernawati Taufik Tri Laksono Tita Damayanti Lestari Tony Hartono Tri Wahyu Suprayogi Tyasningsih, Wiwiek Tyasningsih, Wiwiek Ulfah Juniarti Siregar Utami, Kinanti Putri Vanna Lidya Kharisma Vindo Rossy Pertiwi Wahidan Qodiip Maulana Wahyudi, Nurul Tri Warda Nafalizza Efendi Warsito, Sunaryo Hadi Widi Nugroho Widisuputri, Ni Komang Aprilina Widiyatno, Thomas Valentinus Widya Paramita Lokapirnasari Wijaya, Andi Yasmin Wisnu Nurcahyo Wiwik Misaco Yuniarti Wurlina, W Yonatan Dimascahyo Budianto Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla