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All Journal HAYATI Journal of Biosciences Media Veteriner Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Jurnal Sain Veteriner Jurnal Kebidanan Journal of Biomedicine and Translational Research Majalah Obat Tradisional Indonesian Journal of Cancer Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Jurnal Ilmiah Kedokteran Wijaya Kusuma Universa Medicina Jurnal Penelitian Kesehatan Suara Forikes Journal Of Vocational Health Studies Jurnal Ilmu Kesehatan Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner Care : Jurnal Ilmiah Ilmu Kesehatan JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan Majalah Biomorfologi (Biomorphology Journal) Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Heart Science Journal Journal of Marine and Coastal Science Indian Journal of Forensic Medicine & Toxicology Bioscientist : Jurnal Ilmiah Biologi journal of Basic Medical Veterinary Asian Journal of Health Research Indonesian Journal of Cancer Jurnal Kedokteran Hewan Current Biomedicine Journal of Education Technology and Inovation Strada Jurnal Ilmiah Kesehatan (SJIK) JURNAL KEPERAWATAN Medica Majapahit Folia Medica Indonesiana

Widjiati Widjiati, Widjiati

Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 596972

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Penurunan progesterone-induced blocking factor (PIBF) sebagai penanda preeklampsia Achmad Salman; Budi Wicaksono; Erry Gumilar Dachlan; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 24 No. 1 (2016): Januari - April
Publisher : Universitas Airlangga

Background: Pathogenesis of preeclampsia still a controversial research objects. Progesterone induced blocking factor has protective effects in pregnancy.Objectives: To compare PIBF serum level and placental weight and investigate correlation between both in preeclampsia.Methods: This experimental study used 16 Mus musculus with normal pregnancy and 16 Mus musculus that were injected with anti Qa-2 to create a preeclampsia model. Terminations of were performed in day 16th, followed by ELISA examination for PIBF serum level and placental weight measurement.Result: PIBF serum level significantly reduced on preeclampsia model compared with control (33,21±29,36 ng/ml vs 105,76±59,69 ng/ml; p<0,05). There was no different of placental weight in preeclampsia model compared with control (97,75±50,74 mg vs 116,09±44,45 mg; p>0,05). There was no significant correlation between PIBF serum level and placental weight.Conclusion: Decreased of PIBF serum level could be used as a marker of preeclampsia. Decreased of PIBF serum level may be correlate with placental pathology in preeclampsia.

Comparison of the provision of champedon trunk shell capsule extract and artesunate on placental histopathologic classification in pregnant mice (Mus musculus) malaria model Achmad Yunus; Budi Prasetyo; Erry Gumilar Dachlan; Aty Widyawaruyanti; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 25 No. 3 (2017): December
Publisher : Universitas Airlangga

Objectives: To compare the effect of chemphedon trunk shell capsule extract with artesunate on placental histopathologic classification in pregnant mice malaria’s models.Materials and Methods: This study was a randomized experi-mental laboratory study in BALBc strains mice with controls. A total of 30 pregnant mice were divided into 3 groups. On day 10 each group was infected with P. berghei. Furthermore, on day 11 each group was subjected to thin blood smear examination and subsequent infection when administered antimalarial positive. Group P1 received Chemphedon trunk shell extract 100 mg/kg BW/day per sonde two times per day for 5 days. Group P2 received 36.4 mg artesunate/kg BW/day for 3 days followed by CMC Na per sonde for 2 days, and group P3 received placebo (CMC Na) for 5 days. On day 16 the mice were dissected on 16 days of pregnancy and the placenta was taken and preparations were made to observe histopathological classification of the placenta according to Rogerson. Grouping was performed accor-ding to placental histopathological classification by Rogerson.Results: Groups receiving cemphedon trunk shell capsule extract, artesunate and placebo revealed p=0.004 (p<0.05), showing that there were two groups with significant difference. To determine which group had significant difference, the test was followed by Mann-Whitney post-hoc test. The results showed chempedon trunk shell capsule group and placebo obtained p=0.007 (p<0.05), indicating significance. Artesunate and placebo groups revealed p=0.003 (p<0.05), also indicating significance. The test results of chemphedon trunk shell capsule extract and artesunate groups showed p=0.475 (p<0.05), indicating no significant differences.Conclusion: Placental histopathologic classification on pregnant mice malaria’s model that received antimalarial chemphedon trunk shell capsule extract of 100 mg/kg BW is better than place-bo and equivalent to artesunate of 36.4mg/kg BW.

Why Mozart compositions during pregnancy should be exposed in the night. Study on apoptotic index of Rattus norvegicus offsprings’ brain neurons Eka Nasrur Maulana; Hermanto Tri Joewono; Widjiati Widjiati; Windhu Purnomo
Majalah Obstetri dan Ginekologi Vol. 25 No. 3 (2017): December
Publisher : Universitas Airlangga

Objectives: To analyze the optimal time to expose Mozart compositions(in the night/ dark) by measuring brain neural apoptotic index of Rattus norvegicus offspringsMaterials and Methods: This study used experimental random-ized post test only control group design in pregnant Rattus norvegicus. Subjects were divided into three groups at random, ie control group, 1 (1 hour Mozart exposure in dark) and 2 (1 hour Mozart in light), each comprised 8 females. After delivery, 2 heaviest Rattus norvegicus offsprings were chosen, totally 15 neonates in each group, then they were sacrificed with decapita-tion and the brain was prepared and stained using TUNEL assay method and the index of neurons cell apoptosis was calculated using microscope in 1000x magnification. This study was conducted at the Faculty of Veterinary Medicine, University of Airlangga, after conducting ethical feasibility test.Results: There were no miscarriages, congenital malformation, preterm birth and deaths in all groups. The control group's neural apoptosis index was 55.98 ± 4.12, group one 24.87±3.45 and group two 23.82±3.89. Based on statistical test result, there was no significant difference of apoptosis index between treatment group 1 and treatment group 2, with p value 0,836 but significant difference was found between control group and treatment group 1 (p=0.002) and between control group and treatment group 2 (p=0.009). In dark group we found the lower average of apoptotic index, the lowest apoptotic index, 9 of 15 had the lowest, the heaviest head weight and 4 of 15 subjects had the heaviest head weightConclusion: There was no significant difference between the pro-vision of Mozart music in dark and light on brain neuron of Rattus norvegicus offsprings although lower average in the dark group especially if the extreme value exluded. In the dark group: we found the lower average of apoptotic index, the lowest apoptotic index, biggest proportion of the lowest index, the heaviest head and biggest proportion of heaviest head.

Therapeutic effectiveness of rat bone marrow stem cells in Poly Cystic Ovary Syndrome Mice Model on folliculogenesis, TGF-β, GDF-9 expression, and estrogen, TNF- and androgen Levels Budi Santoso; Agus Sulistyono; Salmon Charles S; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 24 No. 3 (2016): September - December
Publisher : Universitas Airlangga

Objectives: to identify therapeutic effectiveness of Rat Bone Marrow stem cell in PCOS rats model on folliculogenesis, TGF-beta and GDF-9 expression and on estrogen, TNF-a and androgen levels.Material and Methods: this study is a laboratory experimental research with using animal testing. PCOS was induced by the administration of testosterone propionate hormone into 30 mice. The subjects of this study are divided into 2 groups: stem cell group and control group. The mice were injected with testosterone then vaginal swab was performed to determine the mice cycle. After determining mice in anestrous cycle, stem cell was injected. TNF-a was measured with immunohistochemistry and androgen was examined using ELISA. The data was measured by student t-test.Result: The average number of TNF-a expression in control group was lower than stem cell group (5.35 vs 2.34; p= 0.0026). The average androgen level for stem cell group was lower than mean for control group (2.31 vs 0.40; p= 0.0026).Conclusion: In this study of polycystic model mice, stem cell decreased the expression of TNF-a and androgen level

Effect of prenatal Mozart composition on Brain Derived Neurotrophic Factor expression in cerebrum and cerebellum of Rattus norvegicus offspring from Food Restriction 50 model Yulia Putri Permatasari; Widjiati Widjiati; Hermanto Tri Joewono
Majalah Obstetri dan Ginekologi Vol. 26 No. 1 (2018): April
Publisher : Universitas Airlangga

Objectives: To analyze the difference of BDNF expression on Rattus norvegicus offspring FR50 model in cerebrum and cerebellum between Mozart composition stimulation group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animal subjects Rattus norvegicus FR50 model. This study was conducted at animal laboratory, Faculty of Veterinary Medicine, Airlangga University. Animal subjects were divided into Mozart music stimulation group and control. The BDNF expression was analyzed using comparison test, with significancy p<0,05.Results: There was no difference of BDNF expression on Rattus norvegicus offspring FR50 model between Mozart music stimu-lation group and control in cerebrum with p=0.495 (mean Mozart group 6.89+2.52 vs control 6.22+1.99), and cerebellum with p=0.146 (mean Mozart group 7.41+2.67 vs control 5.73+2.45).Conclusions: There was no difference of BDNF expression between Mozart composition stimulation group and control in the cerebrum and cerebellum of Rattus norvegicus offspring FR50 model.

Apoptosis index of cerebrum and cerebellum neuronal cells in Rattus norvegicus neonates born from mothers treated with 50% food restriction during gestation Andita Hapsari; Hermanto Tri Joewono; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 26 No. 2 (2018): August
Publisher : Universitas Airlangga

Objective: To analyze the difference of neuronal apoptotic index on Rattus norvegicus offspring in cerebrum and cerebellum between food restriction 50% group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animals subjects Rattus norvegicus. This study was conducted at animal laboratory, Veterinary Faculty, Universitas Airlangga. Animal subjects were divided into food restriction 50% group and control. The apoptotic index was analyzed using comparison test, with significancy p<0.05.Results: There was significant difference of neuronal apoptotic index on Rattus norvegicus offspring between FR 50% group and control in cerebrum with p= 0.002 (mean 6,12±3,51 in FR 50% group, 2,81±2,16 in control group), and cerebellum with p= 0.026 (mean 5,56±3,03 in FR group, 3,43±2,58 in control group).Conclusions: Food restriction 50% during gestation has signifi-cant influence on neuronal apoptotic index in cerebrum and cerebellum of Rattus norvegicus offspring.

Increased Brain Derived Neurothropic Factor in the cerebrum and cerebellum of Rattus norvegicus newborn with exposure to Mozart's music in default sequence compared with the reversed sequence and without exposure during gestation Agustina Hidayati; Hermanto Tri Joewono; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 26 No. 2 (2018): August
Publisher : Universitas Airlangga

Objectives: To analyze the differences in the expression of Brain Derived Neurothropic Factor (BDNF) in Rattus norvegicus cerebrum and cerebellum of newborn between those exposed to Mozart's music composition in default sequence, reverse sequence, and without exposure in the womb.Materials and Methods: Analytical laboratory experimental study with randomized post test only control group design using animal models Rattus norvegicus. The animal models were divided into three groups: control group without any exposure, the treatment groups with exposure to Mozart's music in default sequence and another group in reverse sequence since day 10 of gestation. We used a comparison test in the analysis of BDNF expression.Results: We found significant difference in BDNF expression with p value 0.004 (mean 8.98±1.31 default sequence group, 5.58±3.08 reverse sequence group, 6.80±1.95 control) in the cerebrum. We found significant difference of BDNF expression with p value 0.003 (mean 9.48±1.41 default sequence group, 6.02±3.25 reverse sequence group, 7.14±2.54 control) in the cerebellum. In cerebrum dan cerebellum we found significant difference between standard Mozart’s music and control (cere-brum p=0.018, cerebellum p=0.001), and we found significant difference between standard Mozart’s music and reverse Mozart’s music (cerebrum p=0.001, cerebellum p=0.008) and no significant difference in reverse Mozart and control (cerebrum p=0.264, cerebellum p=0.490)Conclusion: Sequence in Mozart’s music is very important in increase expression of BDNF.

Sambiloto (AS201-01) is better than standard antimalarial drug (DHP) in reducing Toll-Like Receptor 2 (TLR2) on placenta malaria model Masyitah Hamidah; Aty Widyawaruyanti; Widjiati Widjiati; Budi Prasetyo
Majalah Obstetri dan Ginekologi Vol. 26 No. 2 (2018): August
Publisher : Universitas Airlangga

Objectives: To compare the TLR2 expression in the placenta between treated by sambiloto EA-96 fraction tablet (AS201-01) and dihydroartemisinin-piperaquine phosphate (DHP)Materials and Methods: Experimental study using 24 pregnant mice. All sample divided into 4 groups with randomization are uninfected group, Plasmodium infected group and given placebo, sambiloto (AS201-01) and DHP. Then performed surgery and placental sampling were staining with adopting in tunnel assay method to measure the TLR2 expression of placental.Results: The expression of TLR2 in uninfected group has the lowest rate compared to other groups. The infected and placebo treated group has the highest TLR2 expression campared with sambiloto and DHP. The sambiloto group has not differ signi-ficantly with the group uninfected and lower than DHP.Conclusion: Tablet of sambiloto EA-96 fraction (AS201-01) decreased TLR2 expession better than with DHP tablet.

The effect of sambiloto tablet (AS201-01) on placental Chondroitin Sulfate A (CSA) expression of pregnant mice infected by Plasmodium berghei Nasrul Wahdi; Widjiati Widjiati; Aty Widyawaruyanti; Budi Prasetyo
Majalah Obstetri dan Ginekologi Vol. 26 No. 2 (2018): August
Publisher : Universitas Airlangga

Objectives: To determine the effect of Sambiloto tablet (AS201-01) in reducing the placental Chondroitin Sulfate A (CSA) Expression of pregnant mice infected Plasmodiumberghei.Materials and Methods: Experimental study using 24 pregnant mice were divided into 4 groups with randomization. Uninfected group, the placebo group, the Sambiloto tablet (AS201-01) group and the DHP tablet (as a standart drug) group. The last three groups, were infected with P. bergheion day 9th of pregnancy, and the treatment was started at day 11th of pregnancy, and samples were terminated at day 15th of pregnancy by surgery. Placental sampling were stained with Tunnel assay to measure placental CSA antibodies.Results: The placental Chondroitin Sulfate A (CSA) expression. Uninfected group compared to Sambiloto tablet (AS201-01) groups was not significantly different (p>0.05), uninfected group compared with the other treatment groups differ meaningfully (p<0.05). Placebo group compared with all groups significantly different (p<0.05). Sambiloto tablet (AS201-01) group compared to uninfected group (p>0.05) was not significantly different, with another group was significantly different (p<0.05). DHP tablet group compared to all the groups was significantly different (p<0.05).Conclusion: Placental Chondroitin Sulfate A (CSA)expression of mice infected by Plasmodium berghei treated with Sambiloto tablet (AS201-01) lower than DHP tablet.

Comparison of the effect of sambiloto (AS 201-01) tablet and dihydroartemisinin-piperaquine on macrophage MIF expression in mice placenta infected with Plasmodium berghei Desak Ketut Ayu Aryani; Budi Prasetyo; Aty Widyawaruyanti; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 26 No. 3 (2018): December
Publisher : Universitas Airlangga

Objective: To compare the administration of sambiloto tablets (AS201-01) and dihydroartemisinin-piperaquine phosphate tablets in pregnant mice infected by P. berghei on the expression of MIF in the placenta.Materials and Methods: Experimental laboratory research, 24 pregnant mice were divided into 4 groups with randomization, ie. non-infected, placebo, sambiloto (AS201-01) and DHP groups. On day 9 P. berghei was infected, on day 11 the treatment was given, day 15 the surgery was performed, placental samples were taken, immunohistochemical staining was given, and MIF expression was assessed.Results: The expression of MIF in the group not infected with P. berghei had the lowest mean, while the highest mean was found in the placebo group. Uninfected groups were not significantly different from sambiloto (AS201-01) tablet group. Sambiloto tablet group (AS201-01) had lower MIF expression than DHP group, but it was not significantly different. Sambiloto tablet group (AS201-01) showed lower MIF expression than placebo. MIF expression in DHP group was lower than that in placebo group. From the lowest, the MIF expressions were as follows: group not infected with P. berghei, group receiving sambiloto (AS201-01) tablet, DHP group and placebo group.Conclusion: MIF expression in the placenta of pregnant mice infected with P. berghei and receiving sambiloto (AS20-01) tablets was not different from those receiving DHP tablets.

Co-Authors . AULANI’AM Achmad Salman Achmad Salman Faridz, Achmad Salman Achmad Yunus Adi Setyawan Prianto Agung Budianto Achmad, Agung Budianto Agus Sulistyono Agustina Hidayati Agustina Mar'atus Sholichah Agustina, Widayati Ahmad Rukhani Lutfi Alfiah Hayati Ali Madinah Ali Medina Ali Medina Aliyah, Suhartatik Amila, Ukhti Mukminah ilmi Ancha Ayu Amishinta Andita Hapsari Anike Rachmawati Anike Rachmawati Anindya Marcha Angelina Djianto Anna Surgean Veterini Annis Catur Adi Annise Proboningrat Anwar Fauzi Ardy, Carolina Arief Boediono Arimbi Askandar Tjokroprawiro Aswin, R. Haryanto Aty Widyawaruyanti Aucky Hinting Ayu Putri Yani Azami D Azinar Bambang Poernomo Bambang Purwanto Bambang Sektiari Lukiswanto Bambang Wahjuprajitno Berlina, Cyrcilia Relita Binta Dwi Novitasari Boedi Setiawan Budi I Santoso Budi Prasetyo Budi Santoso Budi Santoso Budi Santoso Budi Susetyo Pikir Budi Susetyo Pikir Budi Utomo Budi Utomo Budi Wicaksono Budi Wicaksono Chairul Anwar Darsono, Roesno Desak Ketut Ayu Aryani Desi Wulansari Desi Wulansari Dessy Hidayati Fajrin Dewita Dewita Dhesy Kartikasari Dian Sukmawati Diana Estu Rumahastuti Dina Helianti Dwi Gustin, Mandasari Dwi Nurdi Puspita Sari Dwi Puji Wijayanti Eighty M Kurniawati Eighty Mardiyan K, Eighty Eka Nasrur Maulana Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Setyaningsih endra, priawasana Epy Muhammad Luqman Erma Safitri Ermawati Ermawati Erry Gumilar Dachlan, Erry Gumilar Dachlan, Erry Gumilar Exma Mu'tatal Hikmah Faizah, Zakiyatul Fauzan Djunaidi Fedik A Rantam Fedik Abdul Rantam Fitria Desky Gandul Atik Yuliani Gatut Hardianto, Gatut H.M Bahkriansyah Hamdani Lunardhi Hana Eliyani Hani Plumeriastuti Hany Aryani Puspita Hari Basuki Notobroto Hari Paraton Hariadi, Mas'ud Harry Mangasi Binsar Panjaitan Haspita Rizki Syurya Handini Hendy Hendarto HENI RACHMAWATI HERAWATI, LILIK Herlina Alvianti N Herlina Puji Angesti Hermanto Hermanto Hermanto Hermanto Hermanto T Joewono Hermanto TJ Hermanto Tri J Hermanto Tri Joewono Hermanto Tri Juwono Heru Santoso Wahito Nugroho Hidajati, Nove Hidayatullah, Amiruddin Ika WAHYUNI IMAM MUSTOFA Imam Mustofa INDRA YULIATI, INDRA Inu Mulyantoro Iwan Sahrial Hamid Jeanita, Winny Jimmy Yanur Annas Jojor Sihotang Joseph Chandra Relmasira Kartikasari, Dhesy Khasanah, Rima Nur Kuncorojakti, Suryo Kurniawati, Sepvian Dewi Kusumawardani, Emi Laba Mahaputra Laili Muninggar Laksmi Sulmartiwi Lilik Maslachah Machmudia, Ainun Mafruchati, Maslichah Masyitah Hamidah Meitria Syahadatina Noor Meles, Dewa Ketut Mikra Latisfian Mikra Latisfian Mikra Latisfian Muhammad Miftahussurur Mulyanto Mulyanto Mulyanto Mulyanto Muninggar, Laili MY Ardianta W N Cininta M Nasrul Wahdi Nenny Harijani Ngakan Made Rai Widjaja Ngakan Made Rai Widjaja Ningrum, Nyna Puspa Noor Pramono Noor Pramono Noor Pramono Noor Pramono Noerpramana Nur Anindya Syamsudi Nur AR Widiatmoko Nur Cholila Nur Laila Faizah Nurbayatin, Anita Nursadida, Milla Nurvy Alief Aidillah Nusdianto Triakoso Nyna Puspa Ningrum Oktoria Indrapraja Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Liben Paulus Sugianto, Paulus Permatasari, Dian Ayu Pipit Sri Estuning Rahayu Pitria Permatasari Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Pudji Srianto Rachmad Suhanda Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Ratriana Via Parasti Raudatul Hikmah Rehatta, Nancy Margarita Relly Yanuari Primariawan, Relly Yanuari Rimayanti R Rimayanti R, Rimayanti Rimayanti Rimayanti RINA YUDIWATI BM, RINA Risna Y, Dyah Ayu Roro Rochmah Kurnijasanti Romi Ermawan Romi Ermawan, Romi Rusyawardani, Aldis Ingrid S.Pd. M Kes I Ketut Sudiana . Salmon Charles Pardomuan Tua Siahaan Sami, Abdul Sari, Gadis Meinar Sarmanu, Sarmanu Sarudji, Suryanie Seindy Glamour Shabira, Zahra Siti Nur Kholifah Siti Nur Kholifah Siti Nur Kholifah SOETJIPTO Sofiyanti Miftakhurohmah Sri Mulyati Sri Mulyati Sri Mumpuni Sri Mumpuni Sri Pantja Madyawati Sri Pantja Madyawati, Sri Sri Pantja Madyawati, Sri Pantja Sri Ratna Dwiningsih Subijanto Marto Soedarmo Suherni Susilowati Sulistiawati Sulistiawati Sutiman Bambang Sumitro Suwarno . Suzanita Utama Syarief T. Hidayat Syarief Thaufik Hidayat Syifa Fauziyah Tias, Eriza Putri Ayu Ning Tinta Julianawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Hartini Yuliawati Tri HS Hadi Tri Purwanti Tri Wahyu Suprayogi Trilas Sardjito TRINIL SUSILOWATI Tyasningsih, Wiwiek Ukhti Mukminah ilmi Amila Varianida Veterini Virki Widoyanti Viski Fitri Hendrawan Wahyuni, Retno Sri Wicaksono, M Galih Widodo Jatim P Widya Paramita Lokapirnasari Windhu Purnomo Wiranata, Try Ardhi Wurlina, W Yaner, Nurul Ramadhani Yeni Dhamayanti Yoes Priyatna Dachlan Yudhana, Aditya Yudi Her Oktaviono Yudi Her Oktaviono, Yudi Her Yuli Trisetiyono Yulia Putri Permatasari Yulianna Puspitasari

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