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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Folia Medica Indonesiana Indonesian Journal of Cancer The Indonesian Biomedical Journal Universa Medicina Journal Of Vocational Health Studies Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner Care : Jurnal Ilmiah Ilmu Kesehatan Ovozoa: Journal of Animal Reproduction Bioscientist : Jurnal Ilmiah Biologi journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Current Biomedicine
Widjiati Widjiati, Widjiati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Pengaruh urea dalam media maturasi in vitro terhadap tingkat maturasi oosit sapi Kurniawati, Sepvian Dewi; Sarudji, Suryanie; Widjiati, Widjiati
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.46-52

Abstract

This study was aimed to determine the effect of urea in maturation medium on in vitro oocyte maturation rate. The medium used was TCM-199 added with Hepes, NaHCO3, Kanamycin 0.15 IU/mL, PMSG, 0.15 IU/mL hCG, and 10% FBS. Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. Each petri dish contained three drops of maturation medium (300 µl/drops) according to the groups. Microdrops were coated with mineral oil and then incubated in a 5% CO2 incubator, at 39 ËšC with maximum humidity. Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. The result showed that the oocyte maturation rates of P0, P1, and P2 were 51.25, 52.43 (p >0.05), and 46.88 % (p <0.05) respectively. It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro.
Efek pemberian α-tokoferol terhadap jumlah sel spermatogenik dan sel Leydig pada tikus putih (Rattus norvegicus) yang dipapar 2,3,7,8 Tetrachlorodibenzo-p-dioxin Machmudia, Ainun; Eliyani, Hana; Widjiati, Widjiati; Wurlina, Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.72-80

Abstract

 This research aimed to determine the effect of α-tocopherol on the count of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (dioxin). Dioxin is an endocrine-disrupting agent that adversely affects reproductive health, while α-tocopherol maintains fertility. This research used 25 rats aged 10-12 weeks weighing 150-200 grams. Rats were divided into five groups (K, P0, P1, P2 and P3). The K (control group) was administered with corn oil 1 ml/day. P0 was exposed to 700 ng/kg/day dioxin. P1, P2 and P3 was exposed to dioxin at a dose of 700 ng/kg/day and administered with α-tocopherol at a dose of 77, 140 and 259 mg/kg/day respectively. Dioxin exposure, corn oil and α-tocopherol administration were conducted orally for 20 days. On day-21, all rats were sacrificed for histological slides preparation of testicles with hematoxylin-eosin staining. Data were analyzed with analysis of variance and continued with the Duncan test. The results indicated that exposure to dioxin caused a decrease in the number of spermatogenic and Leydig cells. The administration of α-tocopherol at a dose of 140 mg/kg/day eliminated the effect of reducing the number of spermatogenic and Leydig cells caused by exposure to dioxin. The conclusion was the administration of α-tocopherol at 140 mg/kg/day was effective in maintaining the number of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin.
THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Kartikasari, Dhesy; Mulyati, Sri; Utama, Suzanita; srianto, Pudji; Widjiati, Widjiati; Plumeriastuti, Hani
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.15283

Abstract

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis.A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL.The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies.Positive results showed a brownish color on the oocyte and its cumulus.The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased.It can be concluded that the addition of urea does not affect the level of apoptosis.
Efek Kadar Urea Nitrogen Darah (BUN) pada Tikus Putih (Rattus norvegicus) Bunting Setelah Pemberian Monosodium Glutamat (MSG) Organik dan Sintetis Tias, Eriza Putri Ayu Ning; Restiadi, Tjuk Imam; Widjiati, Widjiati; Wicaksono, M Galih; Setyaningsih, Endang
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15781

Abstract

This study aims to explores blood urea nitrogen (BUN) levels in Wistar rats exposed to synthetic and organic MSG to identify potential renoprotective effects. The study design involved a control group (K) that was fed a normal diet only, and six treatment groups (P1–P6) that received doses of organic or synthetic MSG at 60, 120, or 240 mg/kg BW for 19 days. Each group consisted of six rats. MSG was administered orally and BUN levels were measured using the colorimetric method. The results showed no significant difference in BUN levels between the control group and the treatment groups (p > 0.05). The highest BUN levels were found in the control group, while the lowest levels were found in the group that received the highest dose of synthetic MSG (240 mg/kg BW). Additionally, BUN levels were lower with organic MSG at 120 and 240 mg/kg BW than with the 60 mg/kg BW dose, suggesting that bioactive compounds in tempeh and pineapple bromelain may have renoprotective effects. While the lowest BUN levels were observed in the group receiving the highest dose of synthetic MSG (240 mg/kg BW), this may be due to reduced protein intake or other metabolic adaptations rather than safety factors. Synthetic MSG at a dose of 240 mg/kg BW produced the lowest BUN levels, which may indicate reduced protein intake or other metabolic adaptations, rather than safety factors.
MUSIK MOZART MEMILIKI JUMLAH SEL GLIA TERBANYAK PADA CEREBRUM DAN CEREBELLUM RATTUS NORVEGICUS BARU LAHIR DIBANDINGKAN DENGAN GAMELAN JAWA, SUNDA DAN BALI SELAMA KEHAMILAN Khasanah, Rima Nur; Joewono, Hermanto Tri; Widjiati, Widjiati
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 9, No 2 (2021): EDITION JULY 2021
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jc.v9i2.1394

Abstract

Intelligence is one of the basic capital in advancing a country. Efforts that can be done during pregnancy is to provide adequate nutrition and stimulation, stimulation that is easily accepted by the fetus is sound and music is the most harmonious combination of sounds. Analyzing the differences in the number of glia cerebrum cells and the newly born Rattus norvegicus cerebellum from mothers exposed to Mozart music, traditional Javanese gamelan music, Sundanese gamelan, Balinese gamelan and those who were not exposed to music during pregnancy. Experimental research with randomized post-test only control group design. Used by experimental animals Rattus norvegicus as a model of music stimulation. Cutting was done with 10 IU PMSG injection, 10 IU HCG and monomating. randomized into 5 groups. The treatment was given from 10-19 days of pregnancy, for 1 hour in the soundproof room, intensity of 65 Db. Children of Rattus norvegicus were decapitated and dissected by the brain then counted the number of glia cells using Hematoksilin - Eosin and analyzed by statistics. There were significant differences in the number of newly born Rattus norvegicus glia cerebrum cells between groups. Mozart group (25.16 ± 1.72; Astrocytes), (26.16 ± 1.50; Oligodendrocytes) and (21.40 ± 4.27; Microglia) were the highest compared to the control group, Javanese gamelan music, Sundanese, and Bali. Average Number of newborn Rattus norvegicus glia cerebellum cells in the Mozart music group (24.48 ± 3.66; Astrocytes), (19.92 ± 3.77; Oligodendrocytes) and (19.92 ± 3.86; Microglia) the highest compared to control groups, Javanese gamelan music, Sundanese gamelan, and Balinese gamelan. The Mozart exposure group had more glia cerebrum and cerebellum cells than those exposed to Javanese, Sundanese, Balinese gamelan and those who were not exposed to music.
MUSIK MOZART MEMILIKI EKSPRESI SYNAPSIN 1 TERTINGGI PADA CEREBRUM DAN CEREBELLUM RATTUS NORVEGICUS BARU LAHIR DIBANDINGKAN DENGAN GAMELAN JAWA, SUNDA DAN BALI SELAMA KEHAMILAN Yaner, Nurul Ramadhani; Joewono, Hermanto Tri; Widjiati, Widjiati
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 9, No 2 (2021): EDITION JULY 2021
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jc.v9i2.1402

Abstract

Stimulation classical music has been shown to influence the increase in brain function and the development of child intelligence. Mozart's classical music has a frequency that can stimulate the growth of synaptic tissue. This research was analyze differences in the expression of synapsin 1 cerebrum and cerebellum Rattus norvegicus was born from mothers exposed to Mozart music, traditional Javanese gamelan music, Sundanese, Balinese and who were not exposed to music during pregnancy. This research was using methods experimental with post test only control group design. The treatment was given to Rattus norvegicus animals from pregnancy day to 10-19, for 1 hour in a soundproof room, intensity of 65 dB. On the 20th day of pregnancy, Rattus norvegicus was sacrificed and 3 of the heaviest, medium and lowest Rattus norvegicus children were selected and the heads of Rattus norvegicus children were decapitated and brain dissection and synapsin 1 expression was calculated using an immunohistochemical method and analyzed with appropriate statistics. Result There were significant differences in the expression of newborn synapsin 1 Rattus norvegicus cerebrum between groups with a value of p = 0.042 (mean in the control group IRS: 2.04 ± 0.26, in the Mozart group IRS: 3.84 ± 1.66, Javanese gamelan group IRS: 2.64 ± 0.51, Sundanese gamelan group IRS: 2.92 ± 0.41, and in the Balinese gamelan group IRS: 2.52 ± 1.18) and so in the cerebellum with a value of p = 0.001 (mean in the control group IRS: 2.02 ± 0.30, in the Mozart group IRS: 4.72 ± 0.54, the Javanese gamelan group was IRS: 3.20 ± 0.82, the Sundanese gamelan group was IRS: 3.12 ± 0.83, and in the Bali gamelan group IRS: 2.08 ± 0.30). Conclusion There are differences in the expression of synapsin 1 of the Rattus norvegicus offspring exposed to Mozart music, gamelan traditional music Javanese, Sundanese and Balinese in the cerebellum but there are no differences in the cerebrum during pregnancy. The highest synapsin expression was in the Mozart group.
Co-Authors Achmad Salman Faridz, Achmad Salman Agung Budianto Achmad, Agung Budianto Amila, Ukhti Mukminah ilmi Annise Proboningrat Ardy, Carolina Arimbi Askandar Tjokroprawiro Bambang Poernomo Berlina, Cyrcilia Relita Budi Susetyo Pikir Budi Utomo Budi Wicaksono Chairul Anwar Darsono, Roesno Dewita Dewita Eighty Mardiyan K, Eighty Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Setyaningsih Erma Safitri Erry Gumilar Dachlan, Erry Gumilar Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Hermanto Hermanto Hermanto Tri Joewono Hidajati, Nove Hidayatullah, Amiruddin IMAM MUSTOFA INDRA YULIATI, INDRA Iwan Sahrial Hamid Jeanita, Winny Jojor Sihotang Kartikasari, Dhesy Khasanah, Rima Nur Kuncorojakti, Suryo Kurniawati, Sepvian Dewi Lilik Maslachah Luqman, Epy Muhammad Machmudia, Ainun Mafruchati, Maslichah Meles, Dewa Ketut Mulyanto Mulyanto Muninggar, Laili Ngakan Made Rai Widjaja Nurbayatin, Anita Nursadida, Milla Nusdianto Triakoso Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Sugianto, Paulus Permatasari, Dian Ayu Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Pudji Srianto Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Relly Yanuari Primariawan, Relly Yanuari Rimayanti Rimayanti RINA YUDIWATI BM, RINA Risna Y, Dyah Ayu Roro Rochmah Kurnijasanti Romi Ermawan, Romi Rusyawardani, Aldis Ingrid Sami, Abdul Sarudji, Suryanie Shabira, Zahra Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistiawati Sulistiawati Suzanita Utama Tias, Eriza Putri Ayu Ning Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Tyasningsih, Wiwiek Viski Fitri Hendrawan Wahyuni, Retno Sri Wicaksono, M Galih Widya Paramita Lokapirnasari Wiranata, Try Ardhi Wurlina, W Yaner, Nurul Ramadhani Yeni Dhamayanti Yudhana, Aditya Yudi Her Oktaviono, Yudi Her Yulianna Puspitasari