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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Indonesian Journal of Cancer The Indonesian Biomedical Journal Universa Medicina Journal Of Vocational Health Studies Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner Care : Jurnal Ilmiah Ilmu Kesehatan Ovozoa: Journal of Animal Reproduction Bioscientist : Jurnal Ilmiah Biologi journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Current Biomedicine Journal of Education Technology and Inovation Folia Medica Indonesiana
Widjiati Widjiati, Widjiati
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Kartikasari, Dhesy; Mulyati, Sri; Utama, Suzanita; srianto, Pudji; Widjiati, Widjiati; Plumeriastuti, Hani
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.15283

Abstract

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis.A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL.The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies.Positive results showed a brownish color on the oocyte and its cumulus.The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased.It can be concluded that the addition of urea does not affect the level of apoptosis.
Efek Kadar Urea Nitrogen Darah (BUN) pada Tikus Putih (Rattus norvegicus) Bunting Setelah Pemberian Monosodium Glutamat (MSG) Organik dan Sintetis Tias, Eriza Putri Ayu Ning; Restiadi, Tjuk Imam; Widjiati, Widjiati; Wicaksono, M Galih; Setyaningsih, Endang
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15781

Abstract

This study aims to explores blood urea nitrogen (BUN) levels in Wistar rats exposed to synthetic and organic MSG to identify potential renoprotective effects. The study design involved a control group (K) that was fed a normal diet only, and six treatment groups (P1–P6) that received doses of organic or synthetic MSG at 60, 120, or 240 mg/kg BW for 19 days. Each group consisted of six rats. MSG was administered orally and BUN levels were measured using the colorimetric method. The results showed no significant difference in BUN levels between the control group and the treatment groups (p > 0.05). The highest BUN levels were found in the control group, while the lowest levels were found in the group that received the highest dose of synthetic MSG (240 mg/kg BW). Additionally, BUN levels were lower with organic MSG at 120 and 240 mg/kg BW than with the 60 mg/kg BW dose, suggesting that bioactive compounds in tempeh and pineapple bromelain may have renoprotective effects. While the lowest BUN levels were observed in the group receiving the highest dose of synthetic MSG (240 mg/kg BW), this may be due to reduced protein intake or other metabolic adaptations rather than safety factors. Synthetic MSG at a dose of 240 mg/kg BW produced the lowest BUN levels, which may indicate reduced protein intake or other metabolic adaptations, rather than safety factors.
MUSIK MOZART MEMILIKI JUMLAH SEL GLIA TERBANYAK PADA CEREBRUM DAN CEREBELLUM RATTUS NORVEGICUS BARU LAHIR DIBANDINGKAN DENGAN GAMELAN JAWA, SUNDA DAN BALI SELAMA KEHAMILAN Khasanah, Rima Nur; Joewono, Hermanto Tri; Widjiati, Widjiati
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 9, No 2 (2021): EDITION JULY 2021
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jc.v9i2.1394

Abstract

Intelligence is one of the basic capital in advancing a country. Efforts that can be done during pregnancy is to provide adequate nutrition and stimulation, stimulation that is easily accepted by the fetus is sound and music is the most harmonious combination of sounds. Analyzing the differences in the number of glia cerebrum cells and the newly born Rattus norvegicus cerebellum from mothers exposed to Mozart music, traditional Javanese gamelan music, Sundanese gamelan, Balinese gamelan and those who were not exposed to music during pregnancy. Experimental research with randomized post-test only control group design. Used by experimental animals Rattus norvegicus as a model of music stimulation. Cutting was done with 10 IU PMSG injection, 10 IU HCG and monomating. randomized into 5 groups. The treatment was given from 10-19 days of pregnancy, for 1 hour in the soundproof room, intensity of 65 Db. Children of Rattus norvegicus were decapitated and dissected by the brain then counted the number of glia cells using Hematoksilin - Eosin and analyzed by statistics. There were significant differences in the number of newly born Rattus norvegicus glia cerebrum cells between groups. Mozart group (25.16 ± 1.72; Astrocytes), (26.16 ± 1.50; Oligodendrocytes) and (21.40 ± 4.27; Microglia) were the highest compared to the control group, Javanese gamelan music, Sundanese, and Bali. Average Number of newborn Rattus norvegicus glia cerebellum cells in the Mozart music group (24.48 ± 3.66; Astrocytes), (19.92 ± 3.77; Oligodendrocytes) and (19.92 ± 3.86; Microglia) the highest compared to control groups, Javanese gamelan music, Sundanese gamelan, and Balinese gamelan. The Mozart exposure group had more glia cerebrum and cerebellum cells than those exposed to Javanese, Sundanese, Balinese gamelan and those who were not exposed to music.
MUSIK MOZART MEMILIKI EKSPRESI SYNAPSIN 1 TERTINGGI PADA CEREBRUM DAN CEREBELLUM RATTUS NORVEGICUS BARU LAHIR DIBANDINGKAN DENGAN GAMELAN JAWA, SUNDA DAN BALI SELAMA KEHAMILAN Yaner, Nurul Ramadhani; Joewono, Hermanto Tri; Widjiati, Widjiati
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 9, No 2 (2021): EDITION JULY 2021
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jc.v9i2.1402

Abstract

Stimulation classical music has been shown to influence the increase in brain function and the development of child intelligence. Mozart's classical music has a frequency that can stimulate the growth of synaptic tissue. This research was analyze differences in the expression of synapsin 1 cerebrum and cerebellum Rattus norvegicus was born from mothers exposed to Mozart music, traditional Javanese gamelan music, Sundanese, Balinese and who were not exposed to music during pregnancy. This research was using methods experimental with post test only control group design. The treatment was given to Rattus norvegicus animals from pregnancy day to 10-19, for 1 hour in a soundproof room, intensity of 65 dB. On the 20th day of pregnancy, Rattus norvegicus was sacrificed and 3 of the heaviest, medium and lowest Rattus norvegicus children were selected and the heads of Rattus norvegicus children were decapitated and brain dissection and synapsin 1 expression was calculated using an immunohistochemical method and analyzed with appropriate statistics. Result There were significant differences in the expression of newborn synapsin 1 Rattus norvegicus cerebrum between groups with a value of p = 0.042 (mean in the control group IRS: 2.04 ± 0.26, in the Mozart group IRS: 3.84 ± 1.66, Javanese gamelan group IRS: 2.64 ± 0.51, Sundanese gamelan group IRS: 2.92 ± 0.41, and in the Balinese gamelan group IRS: 2.52 ± 1.18) and so in the cerebellum with a value of p = 0.001 (mean in the control group IRS: 2.02 ± 0.30, in the Mozart group IRS: 4.72 ± 0.54, the Javanese gamelan group was IRS: 3.20 ± 0.82, the Sundanese gamelan group was IRS: 3.12 ± 0.83, and in the Bali gamelan group IRS: 2.08 ± 0.30). Conclusion There are differences in the expression of synapsin 1 of the Rattus norvegicus offspring exposed to Mozart music, gamelan traditional music Javanese, Sundanese and Balinese in the cerebellum but there are no differences in the cerebrum during pregnancy. The highest synapsin expression was in the Mozart group.
Radiation effect of wireless fidelity (wi-fi) on oocyte number of oocyte stimulation in mice (mus musculus) Nurbayatin, Anita; Widjiati, Widjiati; Primariawan, Relly Yanuari; Poernomo, Bambang; Sulistiawati, Sulistiawati; Yudiwati, Rina
Folia Medica Indonesiana Vol. 53, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Infertility is a problem experienced by some women and men around the world. Most infertility problems in women is caused by impaired reproductive organs or disrupted ovulation.. One factor that causes impaired oocyte maturation is wireless fidelity (Wi-Fi) radiation which has a radio frequency field of 2.45 GHz. The radiation may increase the activity of free radical cells through the fenton reaction pathways that cause infertility because of the disrupted oocyte development. This study aimed to determine the effect of Wi-Fi radiation on the number of oocytes. This was an experimental study using control group design. The subjects were 32 female mice selected through complete random sampling and divided into two groups: control (R0) and exposure (R1). Each group consisted of 16 mice. The Wi-Fi radio frequency used was 2.5 GHz. Mice (R1) were placed closer to Wi-Fi source (± 15cm), and there were two types of laptop PCs and 3G mobile phones connected to internet placed next to mice for 15 hours/28 days. The mice underwent a simultaneous cycle with intraperitoneal injection of PMSG and HCG. Furthermore, the mice were mated with vasectomized male monomatingly to induce ovulation. The fertilization pouch in both oviducts were observed for oocyte collection. The number of oocytes was calculated using an inverted microscope. There was a difference in the number of oocytes between control and exposure group. Statistical tests were analyzed using Mann Whitney U and resulted in significant values (p value = 0.00). No oocytes count in exposure group. In other words, the group underwent anovulation. In conclusion, Wi-Fi radiation affected the number of oocyte stimulation in mice. Therefore, it was important to minimize the risk factors that trigger electromagnetic radiation on reproductive health.
Effects of Red Fruit (Pandanus conoideus Lam) Oil on Malondialdehyde Level and Spermatozoa Quality in Mice (Mus musculus) Exposed to Monosodium Glutamate Agustina, Widayati; Widjiati, Widjiati; Hayati, Alfiah
Folia Medica Indonesiana Vol. 54, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

This study aimed to determine the effects of red fruit (Pandanus conoideus Lam) oil on MDA levels and spermatozoa quality in mice (Mus musculus) exposed to MSG. The quality includes motility, viability, concentration, and morphology of spermatozoa. This experimental study used randomized post-test only control group design. The subjects of this study were 25 mice (Mus musculus), divided into 5 groups (5 mice per group). K- group received distilled water for 35 days. K+ group received 4 mg/g BW MSG for 21 days. P1, P2, and P3 treatment groups received 4 mg/g BW MSG for 21 days and 0.02; 0.04; 0.08 ml/g BW red fruit oil, respectively, from day 22 to 35. The results showed that mean spermatozoa morphology in K-, K+, P1, P2, P3 groups were as follows: 0.86; 0.56; 0.67; 0.61; and 0.87 (%). The spermatozoa concentrations were sequentially as follows: 21; 10; 15; 32,8,19 (107 cells/ml). The spermatozoa's vitalities were as follows: 0,64; 0,14; 0,24; P2: 0.36; 0.68 (%). MDA levels were respectively: 0.29; 0.60; 0.35; 0.23; and 0.19 (nm). As a conclusion, testicular MDA levels in mice exposed to MSG and given with red fruit oil were lower than those in mice exposed to MSG without receiving red fruit oil. The quality of spermatozoa in mice exposed to MSG and receiving red fruit oil was higher than that of mice exposed to MSG without being given with red fruit oil.
Effects of Folic Acid Intake during Pregnancy whose Mother was Exposed to Cigarette Smoke towards Brain Neurons Apoptosis Index and Quantity of Mice (Mus musculus) Offspring Ningrum, Nyna Puspa; Joewono, Hermanto Tri; Widjiati, Widjiati
Folia Medica Indonesiana Vol. 54, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Folic acid contains 5-methyltetrahydrofolate (5-MTHF), one of antioxidants that can be used to inhibit reaction caused by cigarette smoke exposure. Folic acid is able to inhibit the levels of Reactive Oxygen Species (ROS) so as to reduce the occurrence of neuronal apoptosis. The objectives of this study was to determine the effect of folic acid intake during pregnancy whose mother was exposed to cigarette smoke on brain neurons apoptosis index and quantity of mice (Mus musculus) offspring. This study was an experimental laboratory study with posttest-only control group design. The subjects were 24 female mice divided into 4 groups consisting of one control group and three treatment groups. Sampling used simple random sampling, each group consisting of 6 animals. Control group 1 was not given treatment during pregnancy, group 2 was given with folic acid orally on days 0-17 of gestational age as much as 0.06 mg/day/mice. Group 3 was given with cigarette smoke exposure on days 7-17 of gestational age, amounting to 2 cigarettes/day. Group 4 was given with cigarette smoke exposure on days 7-17 of gestational age, amounting to 2 cigarettes/day and folic acid orally on days 0-17 of gestational age as much as 0.06 mg/day/mice. The result showed significant differences in all variables. In neurons apoptosis index, there were significant differences between groups 1 and 3, groups 2 and 3, groups 3 and 4, significant differences in the quantity of neurons between groups 1 and 3, groups 2 and 3, groups 2 and 4. The study concluded that there were significant difference in neural apoptosis index between all groups, and there were also significant differences in the quantity of neurons between all groups.
The Effect of Methylmercury Exposure on Astrocyte of Cerebellar Cortex of White Rats (Rattus novergicus) Sugianto, Paulus; Pardede, Sabrina Melisa; Widjaja, Ngakan Made Rai; Widjiati, Widjiati
Folia Medica Indonesiana Vol. 55, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

The aim of this research was to investigate the effect of different dose methylmercury (II) chloride on astrocyte in cerebellar cortex of white rat (Rattus norvegicus) exposed. This study used randomized control design using 15 adult female Wistar rats weight 180-200 g of body weight. Before treatment the white rats was adapted in a week, then randomly divided into 3 groups each consist of 5 rats. P0 as control were given 0.5 ml aquades, P1 and P2 were given 0.1 and 0.2 mg/kg/day respectively. All groups were given treatment per oral in 30 days with sonde. The data was analyzed by ANOVA, Duncan's multiple range (Duncan's Multiple Range Test). White rats exposed by methylmercury (II) chloride, had a significant differences in the percentage of necrotic astrocyte (p<0.05). Methylmercury chloride exposure increases the number of necrotic astrocytes on white rat.
Nanocurcumin Enhances Antioxidant Defense through GPx Upregulation in Ovarian Granulosa Cells of Endometriosis Mouse Model Handini, Haspita Rizki Syurya; Hendarto, Hendy; Purwanto, Bambang; Widjiati, Widjiati
The Indonesian Biomedical Journal Vol 17, No 6 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i6.3846

Abstract

BACKGROUND: Endometriosis impairs female reproductive function through oxidative stress and apoptosis, reducing oocyte quality and causing infertility. Current therapies are limited by suboptimal efficacy and side effects, including ovulation suppression. Curcumin offers antioxidant and anti-inflammatory benefits but has low bioavailability and poor solubility, which can be improved through nanoparticle formulation. Although nanocurcumin is suggested to act through multiple pathways, its mechanisms remain unclear. This study was conducted to evaluate the antioxidant and anti-apoptotic effects of nanocurcumin in a mouse model of endometriosis.METHODS: Thirty-five mice were allocated into five groups and induced to develop endometriosis using cyclosporine A, ethinyl estradiol, and human endometrial tissue. Nanocurcumin was formulated at three particle sizes (3.71; 3.98; and 25.60 nm) and administered orally at doses of 50, 100, or 200 mg/kg/day for 14 days. After treatment, the mice were euthanized, and ovarian tissues were collected for immunohistochemical analysis of glutathione peroxidase (GPx) and B-cell lymphoma-2 (Bcl-2) expression.RESULTS: The highest GPx expression was observed in the group receiving 50 mg/kg/day nanocurcumin (mean±SD= 6.31±1.97; p=0.042). The lowest expression of Bcl-2 was observed in control group with no treatment (mean±SD=4.15±2.48; p=0.582). Nanocurcumin administration significantly increased GPx expression in a dose-dependent manner compared with the untreated group, while no significant differences were found in Bcl-2 expression.CONCLUSION: Nanocurcumin increases GPx expression, particularly at 50 mg/kg/day, indicating its potential as an antioxidant in reducing oxidative damage associated with endometriosis. However, nanocurcumin did not significantly influence Bcl-2 expression. These findings support nanocurcumin’s role as an effective antioxidant agent in protecting ovarian granulosa cells in endometriosis.KEYWORDS: nanocurcumin, GPx, Bcl-2, endometriosis, granulosa cells
Enhancing 21st Century Skills through Role-Playing in Economics Classrooms: A Quasi-Experimental Study Dwi Gustin, Mandasari; Endra, Priawasana; Aliyah, Suhartatik; Widjiati, Widjiati
Journal of Education Technology and Inovation Vol. 8 No. 2 (2025): Journal of Education Technology and Inovation (December)
Publisher : Program Pasca Sarjana, Universias PGRI Argopuro Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/jeti.v8i2.2623

Abstract

The integration of 21st-century skills into economics education is essential to prepare students for complex global challenges. This quasi-experimental study examined the effectiveness of role-playing pedagogy in enhancing critical thinking, collaboration, and communication skills among senior high school students in Jember, Indonesia. A total of 160 eleventh-grade students participated, divided into an experimental group (n = 80) receiving role-playing instruction and a control group (n = 80) taught through traditional lectures. Data were collected using validated rubrics and analyzed with SPSS 26.0, employing independent samples t-tests and ANCOVA with pretest scores as covariates. Results revealed that the experimental group significantly outperformed the control group across all measured domains, with large effect sizes (Cohen’s d > 1.0). Specifically, role-playing was found to foster deeper cognitive engagement, collaborative interactions, and effective communication within simulated economic contexts. These findings confirm the pedagogical potential of role-playing as a transformative instructional strategy that aligns with experiential and constructivist learning theories. The study contributes to the growing evidence base advocating for active learning approaches in secondary education and highlights the importance of embedding authentic, simulation-based activities into economics curricula. Implications for practice suggest that integrating role-playing can enhance both academic outcomes and transversal competencies, equipping students with critical skills necessary for future academic and professional success
Co-Authors Achmad Salman Faridz, Achmad Salman Agung Budianto Achmad, Agung Budianto Agustina, Widayati Alfiah Hayati Aliyah, Suhartatik Amila, Ukhti Mukminah ilmi Annise Proboningrat Ardy, Carolina Arimbi Askandar Tjokroprawiro Bambang Poernomo Bambang Purwanto Berlina, Cyrcilia Relita Budi Susetyo Pikir Budi Utomo Budi Wicaksono Chairul Anwar Darsono, Roesno Dewita Dewita Dwi Gustin, Mandasari Eighty Mardiyan K, Eighty Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Setyaningsih endra, priawasana Erma Safitri Erry Gumilar Dachlan, Erry Gumilar Gandul Atik Yuliani Hana Eliyani Handini, Haspita Rizki Syurya Hani Plumeriastuti Hariadi, Mas'ud Hendy Hendarto Hermanto Hermanto Hermanto Tri Joewono Hidajati, Nove Hidayatullah, Amiruddin IMAM MUSTOFA INDRA YULIATI, INDRA Iwan Sahrial Hamid Jeanita, Winny Jojor Sihotang Kartikasari, Dhesy Khasanah, Rima Nur Kuncorojakti, Suryo Kurniawati, Sepvian Dewi Lilik Maslachah Luqman, Epy Muhammad Machmudia, Ainun Mafruchati, Maslichah Meles, Dewa Ketut Mulyanto Mulyanto Muninggar, Laili Ngakan Made Rai Widjaja Ningrum, Nyna Puspa Nurbayatin, Anita Nursadida, Milla Nusdianto Triakoso Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Sugianto, Paulus Permatasari, Dian Ayu Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Pudji Srianto Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Relly Yanuari Primariawan, Relly Yanuari Rimayanti Rimayanti RINA YUDIWATI BM, RINA Risna Y, Dyah Ayu Roro Rochmah Kurnijasanti Romi Ermawan, Romi Rusyawardani, Aldis Ingrid Sami, Abdul Sarudji, Suryanie Shabira, Zahra Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistiawati Sulistiawati Suzanita Utama Tias, Eriza Putri Ayu Ning Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Tyasningsih, Wiwiek Viski Fitri Hendrawan Wahyuni, Retno Sri Wicaksono, M Galih Widya Paramita Lokapirnasari Wiranata, Try Ardhi Wurlina, W Yaner, Nurul Ramadhani Yeni Dhamayanti Yudhana, Aditya Yudi Her Oktaviono, Yudi Her Yulianna Puspitasari