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All Journal HAYATI Journal of Biosciences Media Veteriner Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Jurnal Sain Veteriner Jurnal Kebidanan Journal of Biomedicine and Translational Research Majalah Obat Tradisional Indonesian Journal of Cancer Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Jurnal Ilmiah Kedokteran Wijaya Kusuma Universa Medicina Jurnal Penelitian Kesehatan Suara Forikes Journal Of Vocational Health Studies Jurnal Ilmu Kesehatan Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner Care : Jurnal Ilmiah Ilmu Kesehatan JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan Majalah Biomorfologi (Biomorphology Journal) Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Heart Science Journal Journal of Marine and Coastal Science Indian Journal of Forensic Medicine & Toxicology Bioscientist : Jurnal Ilmiah Biologi journal of Basic Medical Veterinary Asian Journal of Health Research Indonesian Journal of Cancer Jurnal Kedokteran Hewan Current Biomedicine Journal of Education Technology and Inovation Strada Jurnal Ilmiah Kesehatan (SJIK) JURNAL KEPERAWATAN Medica Majapahit Folia Medica Indonesiana

Widjiati Widjiati, Widjiati

Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 596972

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Pengaruh Suplementasi Ekstrak Daun Kelor (Moringa pterygosperma Gaertn) terhadap Ekspansi Kumulus Oosit dalam Medium Maturasi In Vitro Nur Anindya Syamsudi; Widjiati Widjiati; Hendy Hendarto
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 11, No 1 (2020): Januari 2020
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf11109

Background: Cumulus cells are mediators providing energy transport, micronutrients, and carrier molecules for oocyte development, and mediate the influence of hormones on the oocyte cumulus complex. Cumulus cells play a role during oocyte growth and maturation which shows the quality of oocyte maturation. The use of basic maturation medium with the addition of various substances and compounds has been extensively studied to study oocyte maturation in vitro. Utilization of drugs from natural ingredients has unique advantages because in general cheap prices, have lower toxicity and side effects, and good therapeutic potential. One of the plants that can be used as a source of natural antioxidants, namely Moringa leaves. Objective: Determine the effect of supplementation Moringa pterygosperm Gaertn extract on the expansion of oocyte cumulus in in vitro maturation medium. Method: This research was true experimental with Post Test Only Control Group Design approach. Divided into 4 groups, namely K: control group, P1: treatment of 10 mg / mL Moringa leaf extract, P2: treatment of 15 mg / mL Moringa leaf extract, and P3: treatment of 20 mg / mL Moringa leaf extract. Furthermore oocytes are matured for 24 hours. Cumulus expansion was evaluated according to the level of expansion achieved where the minimum response (+), medium response (++), and maximum response (+++). Statistical analysis used the One Way Anova test and continued post HoC LSD. Results: There was an influence of supplementation Moringa pterygosperm Gaertn extract on the expansion of moderate response cumulus (++) (p = 0.007) and maximum response (+++) (p = 0.000), but there was no effect on the expansion of cumulus minimal response (+) (0.065 ). Doses of 20 mg / mL are effective for cumulus expansion (+++), but not effective for cumulus expansion (+), (++), and control. Conclusion: Supplementation Moringa pterygosperm Gaertn extract affected the expansion of moderate response (++) and maximum response (+++) oocytes in in vitro maturation medium. Keywords: moringa leaf extract, cumulus expansion, in vitro maturation. ABSTRAK Latar Belakang: Sel kumulus merupakan mediator penyedia transpor energi, mikronutrisi, dan atau molekul pembawa untuk perkembangan oosit, dan menjadi mediasi pengaruh hormon pada kompleks kumulus oosit. Sel kumulus berperan selama pertumbuhan dan maturasi oosit yang menunjukkan kualitas maturasi oosit. Penggunaan medium maturasi dasar dengan penambahan berbagai zat dan senyawa banyak diteliti untuk mempelajari maturasi oosit secara in vitro. Pemanfaatan obat dari bahan alam memiliki kelebihan unik karena pada umumnya harga murah, memiliki toksisitas dan efek samping lebih rendah, serta potensi terapeutik baik. Salah satu tumbuhan yang dapat digunakan sebagai sumber antioksidan alami, yaitu daun kelor. Tujuan: mengetahui pengaruh suplementasi ekstrak daun kelor (Moringa pterygosperma Gaertn) terhadap ekspansi kumulus oosit dalam medium maturasi in vitro. Metode: Jenis penelitian ini adalah eksperimental murni dengan dengan pendekatan Post Test Only Control Group Design. Dibagi menjadi 4 kelompok, yaitu K: kelompok kontrol, P1: perlakuan 10 mg/mL ekstrak daun kelor, P2 : perlakuan 15 mg/mL ekstrak daun kelor, dan P3 : perlakuan 20 mg/mL ekstrak daun kelor. Selanjutnya oosit di maturasi selama 24 jam. Ekspansi kumulus di evaluasi sesuai dengan tingkat ekspansi yang dicapai dimana respon minimal (+), respon sedang (++), dan respon maksimum (+++). Analisa statistik menggunakan uji one way Anova dan dilanjutkan post HoC LSD. Hasil: Terdapat pengaruh suplementasi ekstrak daun kelor terhadap ekspansi kumulus respon sedang (++) (p=0.007) dan respon maksimal (+++) (p=0.000), namun tidak terdapat pengaruh terhadap ekspansi kumulus respon minimal (+) (p=0.065). Kesimpulan : Suplementasi ekstrak daun kelor (Moringa pterygosperma Gaertn) berpengaruh terhadap ekspansi kumulus oosit respon sedang (++) dan respon maksimal (+++) dalam medium maturasi in vitro. Kata kunci: ekstrak daun kelor, ekspansi kumulus, maturasi in vitro.

ES-06 Effect of Rat Bone Marrow Stem Cell Administration to Rats (Rattus norvegicus) Using Infertile Model on Oocyte Apoptosis Widjiati Widjiati
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.053 KB)

Effect of Rat Bone Marrow Stem Cell Administration to Rats (Rattus norvegicus) Using Infertile Model on Oocyte Apoptosis

Mozart Music Increases The Number Of Glial Cells Compared To Indonesia Pop And Religious Music Pipit Sri Estuning Rahayu; Hermanto Hermanto; Widjiati Widjiati
Saintika Medika Vol. 15 No. 2 (2019): December 2019
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/sm.Vol15.SMUMM2.10265

Music stimulation is an important component for prenatal fetal development. Both pop and religious music are easy to listen and widely accepted in Indonesia. This study was to analyze the effect of Mozart, pop, and religious music exposure during pregnancy to the number of glial cells in the brain of Rattus norvegicus offspring. The samples were divided into three groups based on the exposure for each group, namely Mozart, pop, and religious music, duration of 60 minutes with 65dB intensity, initiated on the 10th day of pregnancy for 9 days in the soundproof chamber. Three brains of the offsprings were dissected and prepared for Hematoxylin-Eosin staining counted on 5 fields of view and 400 magnification strength.Different glial cells number of Rattus norvegicus brain between groups were observed. Mozart music (28,29) showed a highest mean and pop music (18,67) showed the lowest mean. Significant difference of the number of brain glial cells between Mozart music compared to pop and religious music groups were observed, with p value <0,005.The number of brain glial cells of Rattus norvegicus offsprings in the Mozart group were significantly higher than those in pop and religious groups.

Synapsis Expression Increased By Mozart’s Music Stimulation Compared With Indonesian Pop And Religious Music Ukhti Mukminah ilmi Amila; Hermanto Tri Joewono; Widjiati Widjiati
Saintika Medika Vol. 16 No. 1 (2020): June 2020
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/sm.Vol16.SMUMM1.10784

Classical music stimulation is a proven method that affect intelligence as well as brain function. Mozart’s classical music is classical music that could stimulate the growth of synaptic networks. To analyze the difference of synapsin expression of the brain of Rattus norvegicus offspring on Mozart’s, pop, religious and without music stimulation during pregnancy. Experimental. Treatments were subjected to Rattus norvegicusas a subject organism, initiated on the 10th day of pregnancy, in 1 hour duration in a soundproof chamber, intensity of 65 dB. On the 19th day of pregnancy the subjects were sacrificed and three offsprings were chosen. The head of the chosen Rattus norvegicusoffsprings were decapitated and the brain subsequently sectioned to calculate synapsin expression by immunohistochemistry method with 400 magnification strength microscope on the 5 field of view. Significant difference of synapsin expression of the brain of Rattus norvegicus offspring was observed between Mozart’s and control group. Significant differences of synapsin expressions were observed in the cerebellum of the newly born Rattus novergicus between Mozart and pop music groups (p = 0,007), Mozart and religious music groups (p = 0,008), Mozart and without music groups (p = 0,004).Synapsin expression of Rattus norvegicus brain offsprings were higher in Mozart music stimulation compared with pop and religious music stimulation

Antibody of goat zona pellucida-3 (gzp3) protein of mice(Mus musculus) block in vitro fertilization of mice as an animal model= Antibodi protein zona pelusida-3 kambing (gZP3) asal mencit(Mus musculus) mencegah ... Imam Mustofa; Laba Mahaputra; Yoes Priyatna Dachlan; Fedik Abdul Rantam; Suwarno .; Widjiati .; Aucky Hinting
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The researchs of immunocontraception have done in ZP3 of several species, but have not been done in ZP3 of goat. In preliminary study, gZP3 protein was effective prohibited of graviditation of mice. The aim of this study was to prove the potency of gZP3 protein to prohibit in vitro fertilization of mice as an animal model. Antibody of gZP3 produced on mice. Immunized mice serum was analyzed using Elisa and Dot blotting method. Antibody of gZP3 supplemented into M-16 media for oocyte incubation, continued with in vitro fertilization. The result showed that antibody titer of immunized mice serum was higher (p

Apium graveolens Prevents Intrauterine Growth Restriction via Suppression of Antiangiogenic Factor Production Exma Mu'tatal Hikmah; Paulus Liben; Widjiati Widjiati
Majalah Obat Tradisional Vol 23, No 2 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Preeclampsia is the worldwide leading cause of fetomaternal morbidity and mortality which involves the placental dysfunction. A poor placentation and formed of non-dilated spiral artery caused utero-placental circulation insufficiency, resulted in inadequate supply of nutrients and oxygen to support normal aerobic growth of the fetus. Apium graveolens or celery has been widely known as antioxidant, antiinflammation and antihypertensive with flavonoid-apigenin as main active compound. Apigenin can inhibit TNF-α, HIF-1α and nitric oxide blocking as major pathophysiological pathway of preeclampsia. This study was aimed to find how the Apium graveolens can improve intrauterine growth and its correlation with suppression of anti-angiogenic factor sFlt-1 in anti-Qa2 preeclampsia animal model. Twenty female BALB/c Mus musculus were divided into 4 groups: control, anti-Qa2 and anti-Qa2 with 500 and 1000 mg/kgBW celery herbs extract. The fetal weights and lengths, placental weights and serum sFlt-1 levels were measured and analyzed with One Way ANOVA and further tested with Least Significance Difference in 95% confidence interval. The result showed a difference between control and treatments group (p≤0.05) with 1000 mg/kgBW significantly increase intrauterine growth and decrease sFlt-1, then there is a negative correlation between intrauterine weight and serum sFlt-1. This study suggests that celery herbs extract (CHE) has an apigenin-flavonoid compound which can prevent intrauterine growth restriction (IUGR) via suppression of antiangiogenic factor production in preeclampsia mice model.

The Differences of Malondialdehyde Serum Level, Expression of Tumor Necrosis Factor Alpha and Vascular Endothelial Growth Factor, and the Area of Endometriotic Implants in Administration of Kebar Grass Extract (Biophytum petersianum) and Green Tea Extract (Camelia sinensis) to Mice Yuli Trisetiyono; Noor Pramono; Syarief Thaufik Hidayat; Widjiati Widjiati
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45237

The pathological pathway of endometriosis remains unclear and involves complex etiologies. Increased oxidative stress is understood to be related to this disease. Oxidative stress produces reactive oxygen species, causes inflammation that is characterized by recruiting lymphocytes and phagocyte activation, produces cytokines that induce oxidation enzyme, and supports epithelium growth. Oxidative stress conjointly will increase angiogenesis and promotes the proliferation of endometriosis tissue within the peritoneal cavity. Kebar grass and green tea contain high antioxidants, are expected to extend antioxidant defense leading to reduced oxidative stress, inflammation, angiogenesis, and reduced endometriosis tissue implants. The objective is to analyze the consequences of Kebar grass and green tea extract to MDA serum level, TNF-α, and VEGF expression, and the area of the endometriotic implants in the mice models. The study was an experiment designed. It has been conducted within the Department of Obstetrics Gynecology, Faculty of Medicine Diponegoro University and Faculty of Veterinary Medicine Airlangga University. Twenty-one mice were divided into three groups, i.e., the first group of mouse models was given Kebar extract 3 mg/day; the second group was assigned green tea extract 1.1 mg/day; therefore the third group was a control group contains the untreated endometriosis mice. Each treatment was given for fourteen days. MDA serum level was measured by specto-photometric examination, the area of the endometriotic implants was measured by computer tracing technique, whereas TNF-α and VEGF expression of endometriotic implants were measured by IHC using Rammele Scale Index (ImmunoReactive Score). The MDA serum levels of the groups treated with Kebar grass extract and green tea extract were significantly lower than the control group (0.09±0.022 mmol, 0.07±0.019 mmol, and 0.30±0.062 mmol, respectively; p=0.001). TNF-α expression of the groups supplied with each treatment also lower than the control groups (2.43±1.521, 3.66±1.422, and 7.26±2.898, respectively; p=0.002). However, VEGF expression was not significantly different between Kebar grass extract group, green tea group, and the control (4.34±2.402, 4.57±1.998, 7.40±3.495, respectively; p=0.089). Finally, the area of the endometriotic implants of the mice models administered with all treatment was smaller than the control group (0.01±0.025 mm2, 8.76±18.776 mm2, and 34.80±13.079 mm2, respectively; p=0.003). Conclusion: Kebar grass extract, as well as green tea extract administration to endometriosis model mice, resulted in lower MDA serum level and TNF-α expression, smaller the area of endometriotic implants compared, but not resulted in a significant difference of VEGF expression.

Oxygen Hyperbaric Exposure Induces GLUT4 Expression Reduction and No Folliculogenesis Alterations in Rat PCOS with Insulin Resistance Model Varianida Veterini; Budi Santoso; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 23 No. 3 (2015): September - Desember 2015
Publisher : Universitas Airlangga

Objectives: To know effect of hyperbaric oxygen therapy to GLUT4 expression and folliculogenesis in rat PCOS with insulin resistent model.Materials and Methods: this is an analytic experimental study used 30 rats induced by testosterone propionate injection 1 mg/100 g body weight daily for 28 consecutive days as a PCOS with insulin resistent model. Animal models divided into 3 groups. Negative control was not given treatment, positive control was given placebo. Treatment group was given hyperbaric oxygen 2.4 ATA 90 minutes 2x5 days. GLUT4 expression determined by immunohystochemistry to m. Gastrocnemius. Hematoxylin Eosin staining to rat ovaries were performed to know differentiation in folliculogenesis. The results were then compared.Results: There were significant decrease of GLUT4 expression in treatment group (mean0,84+0,47)compared to negative and positive control (mean3,96+3,16and3,36+2,17). There were no different folliculogenesis in these groups.Conclusion: Hyperbaric oxygen 2.4 ATA 90 minutes 2x5 days induce decrease of GLUT4 expression and no alterations in folliculogenesis in rat PCOS with insulin resistent model, therefore can not yet be used as alternative therapy in PCOS.

Pemberian Topikal Ekstrak Daun Pepaya (Carica papaya) pada Hewan Coba Mencit (Mus musculus) Bunting Meningkatkan Kepadatan Kolagen Jaringan Vagina Fauzan Djunaidi; Eighty Mardiyan K; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 23 No. 3 (2015): September - Desember 2015
Publisher : Universitas Airlangga

Tujuan: Menganalisis pengaruh pemberian gel ekstrak air daun pepaya terhadap kepadatan kolagen jaringan vagina pasca melahirkan pada hewan coba mencit saat bunting.Bahan dan Metode: Desain penelitian randomized post test only control group,dilakukan di Departemen Embriologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga, pada bulan April-Juni 2015. Terdapat empat kelompok, masing-masing kelompok terdiri dari 8 mencit bunting. Kelompok kontrol (tanpa perlakuan) dan kelompok perlakuan berupa pemberian topikal ekstrak daun pepaya konsentrasi 5%, 10% dan 15%, 1x/hari pervaginam, sejak usia kebuntingan hari ke-12 sampai melahirkan. Lama perlakuan 6-8 hari. Setelah melahirkan, mencit dikorbankan dan diperiksa kepadatan kolagen dengan pewarnaan HE. Uji statistik yang dipakai uji parametrik dan nonparametrik disertai uji komparasi gandaHasil: Kepadatan kolagen berdasarkan skor didapatkan nilai median pada kelompok I = 3,5 (3-4); kelompok II = 4 (4-4); kelompok III = 3 (3-4); kelompok IV = 4 (3-4) dengan uji Kruskal-Wallis (p=0,036) menunjukkan perbedaan signifikan, dilanjutkan uji beda tiap kelompok Mann-Whitney, hasilnya tidak didapatkan perbedaan signifikan antara kelompok kontrol dengan masing-masing kelompok perlakuan. Kepadatan kolagen berdasarkan persentase didapatkan nilai rerata pada kelompok I = 90,64%±3,35; kelompok II = 96,19%±1,34; kelompok III = 87,85%±6,69 dan kelompok IV = 92,08%±4,84. Uji Anova menunjukkan perbedaan signifikan (p=0,032), dilanjutkan uji beda LSD menunjukkan perbedaan bermakna antara kelompok I dan II (p=0,045)Simpulan: pemberian topikal ekstrak daun pepaya konsentrasi 5% pervaginam saat masa kebuntingan mencit meningkatan kepadatan kolagen jaringan vagina pasca melahirkan.

Kurkumin Menurunkan Ekspresi Tumor Necrosis Factor (TNF)-α Kompleks Oosit-Kumulus Sapi pada Kultur dengan Zalir Peritoneum Penderita Infertil Terkait Endometriosis MY Ardianta W; Hendy Hendarto; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 23 No. 3 (2015): September - Desember 2015
Publisher : Universitas Airlangga

Tujuan: Mempelajari pengaruh pemberian kurkumin terhadap ekspresi tumor necrosis factor (TNF)-α kompleks oosit-kumulus (KOK) sapi pada kultur zalir peritoneum penderita infertil dengan endometriosis ringan dan berat.Bahan dan Metode: Penelitian ini merupakan penelitian eksperimental murni yang dilaksanakan di Laboratorium Embrio-logi Fakultas Kedokteran Hewan Universitas Airlangga antara April sampai Agustus 2014. Zalir peritoneum diambil dari penderita infertil dengan endometriosis ringan, endometriosis berat, dan non-endometriosis yang menjalani laparoskopi diagnosis di Klinik Fertilitas Graha Amerta RSUD dr. Sutomo Surabaya, RS Bersalin Putri Surabaya, RSIA Kendangsari Surabaya, RS Universitas Airlangga Surabaya. Masing-masing zalir peritoneum endometriosis ringan dan berat dibagi lagi menjadi zalir peritoneum endometriosis dengan kurkumin dan tanpa kurkumin. Zalir peritoneum non endometriosis dikelompok-kan menjadi zalir peritoneum penderita non endometriosis tanpa dan dengan kurkumin. KOK sapi diambil secara consecutive sampling dari aspirasi folikel antral diameter 3–8 mm dari ovarium sapi yang berasal dari Rumah Potong Hewan Surabaya kemudian secara acak dengan randomisasi sederhana dikultur dalam tujuh kelompok. Kelompok 1 ditempatkan dalam tissue culture medium 199 (TCM-199) saja. Kelompok 2, 3 dan 4 TCM-199 ditambah masing-masing 3% zalir peritoneum penderita infertil dengan endometriosis ringan, berat dan non-endometriosis. Kelompok 5, 6, dan 7 media TCM-199 ditambah masing-masing 3% zalir peritoneum penderita infertil dengan endometriosis ringan, berat dan non-endometriosis dan dengan kurkumin 20 µg/ml.Hasil: Uji Kruskal Wallis menunjukkan perbedaan yang bermakna ekspresi TNF-α pada ketujuh kelompok (p<0,0001). Uji Mann-Whitney juga menunjukkan bahwa ekspresi TNF-α KOK sapi pada kultur kelompok endometriosis ringan dengan kurkumin (ER+C) lebih rendah secara bermakna dibandingkan kelompok endometriosis ringan tanpa kurkumin (ER). Ekspresi TNF-α KOK sapi pada kultur kelompok endometriosis berat dengan kurkumin (EB+C) lebih rendah secara bermakna dibandingkan kelompok endometriosis berat tanpa kurkumin (EB). Uji Mann-Whitney menunjukkan ekspresi TNF-α KOK sapi pada kultur kelompok ER lebih tinggi secara bermakna dibandingkan kontrol ataupun kelompok non endometriosis (NE). Ekspresi TNF-α KOK sapi pada kultur kelompok EB lebih tinggi secara bermakna dibandingkan kontrol ataupun kelompok NE. Ekspresi TNF-α KOK sapi pada kultur kelompok EB tidak didapatkan perbedaan yang bermakna dibandingkan dengan kelompok ER.Simpulan: Pemberian kurkumin dapat dipertimbangkan untuk digunakan sebagai terapi tambahan pada penderita infertil terkait endometriosis.

Co-Authors . AULANI’AM Achmad Salman Achmad Salman Faridz, Achmad Salman Achmad Yunus Adi Setyawan Prianto Agung Budianto Achmad, Agung Budianto Agus Sulistyono Agustina Hidayati Agustina Mar'atus Sholichah Agustina, Widayati Ahmad Rukhani Lutfi Alfiah Hayati Ali Madinah Ali Medina Ali Medina Aliyah, Suhartatik Amila, Ukhti Mukminah ilmi Ancha Ayu Amishinta Andita Hapsari Anike Rachmawati Anike Rachmawati Anindya Marcha Angelina Djianto Anna Surgean Veterini Annis Catur Adi Annise Proboningrat Anwar Fauzi Ardy, Carolina Arief Boediono Arimbi Askandar Tjokroprawiro Aswin, R. Haryanto Aty Widyawaruyanti Aucky Hinting Ayu Putri Yani Azami D Azinar Bambang Poernomo Bambang Purwanto Bambang Sektiari Lukiswanto Bambang Wahjuprajitno Berlina, Cyrcilia Relita Binta Dwi Novitasari Boedi Setiawan Budi I Santoso Budi Prasetyo Budi Santoso Budi Santoso Budi Santoso Budi Susetyo Pikir Budi Susetyo Pikir Budi Utomo Budi Utomo Budi Wicaksono Budi Wicaksono Chairul Anwar Darsono, Roesno Desak Ketut Ayu Aryani Desi Wulansari Desi Wulansari Dessy Hidayati Fajrin Dewita Dewita Dhesy Kartikasari Dian Sukmawati Diana Estu Rumahastuti Dina Helianti Dwi Gustin, Mandasari Dwi Nurdi Puspita Sari Dwi Puji Wijayanti Eighty M Kurniawati Eighty Mardiyan K, Eighty Eka Nasrur Maulana Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Setyaningsih endra, priawasana Epy Muhammad Luqman Erma Safitri Ermawati Ermawati Erry Gumilar Dachlan, Erry Gumilar Dachlan, Erry Gumilar Exma Mu'tatal Hikmah Faizah, Zakiyatul Fauzan Djunaidi Fedik A Rantam Fedik Abdul Rantam Fitria Desky Gandul Atik Yuliani Gatut Hardianto, Gatut H.M Bahkriansyah Hamdani Lunardhi Hana Eliyani Hani Plumeriastuti Hany Aryani Puspita Hari Basuki Notobroto Hari Paraton Hariadi, Mas'ud Harry Mangasi Binsar Panjaitan Haspita Rizki Syurya Handini Hendy Hendarto HENI RACHMAWATI HERAWATI, LILIK Herlina Alvianti N Herlina Puji Angesti Hermanto Hermanto Hermanto Hermanto Hermanto T Joewono Hermanto TJ Hermanto Tri J Hermanto Tri Joewono Hermanto Tri Juwono Heru Santoso Wahito Nugroho Hidajati, Nove Hidayatullah, Amiruddin Ika WAHYUNI IMAM MUSTOFA Imam Mustofa INDRA YULIATI, INDRA Inu Mulyantoro Iwan Sahrial Hamid Jeanita, Winny Jimmy Yanur Annas Jojor Sihotang Joseph Chandra Relmasira Kartikasari, Dhesy Khasanah, Rima Nur Kuncorojakti, Suryo Kurniawati, Sepvian Dewi Kusumawardani, Emi Laba Mahaputra Laili Muninggar Laksmi Sulmartiwi Lilik Maslachah Machmudia, Ainun Mafruchati, Maslichah Masyitah Hamidah Meitria Syahadatina Noor Meles, Dewa Ketut Mikra Latisfian Mikra Latisfian Mikra Latisfian Muhammad Miftahussurur Mulyanto Mulyanto Mulyanto Mulyanto Muninggar, Laili MY Ardianta W N Cininta M Nasrul Wahdi Nenny Harijani Ngakan Made Rai Widjaja Ngakan Made Rai Widjaja Ningrum, Nyna Puspa Noor Pramono Noor Pramono Noor Pramono Noor Pramono Noerpramana Nur Anindya Syamsudi Nur AR Widiatmoko Nur Cholila Nur Laila Faizah Nurbayatin, Anita Nursadida, Milla Nurvy Alief Aidillah Nusdianto Triakoso Nyna Puspa Ningrum Oktoria Indrapraja Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Liben Paulus Sugianto, Paulus Permatasari, Dian Ayu Pipit Sri Estuning Rahayu Pitria Permatasari Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Pudji Srianto Rachmad Suhanda Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Ratriana Via Parasti Raudatul Hikmah Rehatta, Nancy Margarita Relly Yanuari Primariawan, Relly Yanuari Rimayanti R Rimayanti R, Rimayanti Rimayanti Rimayanti RINA YUDIWATI BM, RINA Risna Y, Dyah Ayu Roro Rochmah Kurnijasanti Romi Ermawan Romi Ermawan, Romi Rusyawardani, Aldis Ingrid S.Pd. M Kes I Ketut Sudiana . Salmon Charles Pardomuan Tua Siahaan Sami, Abdul Sari, Gadis Meinar Sarmanu, Sarmanu Sarudji, Suryanie Seindy Glamour Shabira, Zahra Siti Nur Kholifah Siti Nur Kholifah Siti Nur Kholifah SOETJIPTO Sofiyanti Miftakhurohmah Sri Mulyati Sri Mulyati Sri Mumpuni Sri Mumpuni Sri Pantja Madyawati Sri Pantja Madyawati, Sri Sri Pantja Madyawati, Sri Pantja Sri Ratna Dwiningsih Subijanto Marto Soedarmo Suherni Susilowati Sulistiawati Sulistiawati Sutiman Bambang Sumitro Suwarno . Suzanita Utama Syarief T. Hidayat Syarief Thaufik Hidayat Syifa Fauziyah Tias, Eriza Putri Ayu Ning Tinta Julianawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Hartini Yuliawati Tri HS Hadi Tri Purwanti Tri Wahyu Suprayogi Trilas Sardjito TRINIL SUSILOWATI Tyasningsih, Wiwiek Ukhti Mukminah ilmi Amila Varianida Veterini Virki Widoyanti Viski Fitri Hendrawan Wahyuni, Retno Sri Wicaksono, M Galih Widodo Jatim P Widya Paramita Lokapirnasari Windhu Purnomo Wiranata, Try Ardhi Wurlina, W Yaner, Nurul Ramadhani Yeni Dhamayanti Yoes Priyatna Dachlan Yudhana, Aditya Yudi Her Oktaviono Yudi Her Oktaviono, Yudi Her Yuli Trisetiyono Yulia Putri Permatasari Yulianna Puspitasari

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