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Prevalence and Risk Factor of Repeat Breeder Syndrome in Dairy Cows in KUD Bebarengan Anggayuh Tentrem Urip, Batu City, East Java Province Frida Aulya Arningdiah; Rahmatullah, Aldin Akbar; Cindy Ercha Aulia Putri; Mufasirin; Hermin Ratnani; Rimayanti; Sri Pantja Madyawati; Tita Damayanti Lestari; Muhammad Al-Syafiq bin Abdul Halim; Nuurin Ajrin Karim
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.232-242

Abstract

This study aims to contribute valuable insights to readers and serve as a reference for dairy farmers looking to enhance their operations. By examining the prevalence and risk factors associated with repeat breeder syndrome in dairy cows within the Village Unit Cooperation (KUD) Bebarengan Anggayuh Tentrem Urip, Batu City, East Java Province, this study aims to aid in preventing such occurrences and ultimately improve dairy cow productivity. The survey method was employed, utilizing data sourced from cooperative and farmer records, as well as observations, interviews, and questionnaire responses. Analysis revealed that out of 50 farmers owning a total of 281 cows, 30 farmers possessed a combined total of 58 dairy cows classified as repeat breeders. Consequently, the prevalence rate of repeat breeders among farmers was found to be 20.64%. Descriptive analysis was conducted on the collected data, with additional SPSS Chi-Square (X²) analysis performed to identify risk factors associated with repeat breeders. These risk factors include breeder experience, knowledge of reproduction and estrus phases, communication with artificial inseminators and veterinarians, housing conditions, as well as factors related to livestock feed and water.
Epidemiological Studies of Subclinical Mastitis in Dairy Goats in Lumajang Regency, East Java, Indonesia Fatmawati, Mira Fatmawati; Lucia Tri Suwanti; Mufasirin; Mirni Lamid; Nunuk Dyah Retno Lastuti; Endang Suprihati; Mustofa Helmi Effendi; Anam Al Arif; Widi Nugroho; Masdiana C Padaga
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 3 (2023): December 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.03.07

Abstract

Subclinical mastitis is an economically important disease in dairy goat farming. Lumajang is a center for dairy goats in East Java. However, routine examination of subclinical mastitis for screening tests has no data. This study aimed to provide prevalence data and identification factors that influenced subclinical mastitis. Therefore, this study was carried out as part of the national surveillance program. The research design is a cross-sectional study with simple random sampling. Samples came from 19 farms with a total individual sample of 224 dairy goats. Detection of subclinical mastitis using an undirect test with the California Mastitis Test (CMT). Identification of management factors and characteristics that can influence subclinical mastitis in dairy goat farms using a structured questionnaire. The results showed that the prevalence of subclinical mastitis in Lumajang District was 36.2%. There was a significant difference in subclinical mastitis in each village (p<0.05). The highest prevalence of subclinical mastitis was in Kandangtepus village. A farmer's characteristic factor that significantly influences subclinical mastitis is the farmer's experience. The aspect of livestock management that significantly affects subclinical mastitis is structured work management. Subclinical mastitis was prevalent in dairy goats in Lumajang District and requires prevention efforts. Prevention approach through education for farmers to increase knowledge, attitude, and socialization to improve livestock management.
PENGARUH PEMBERIAN MADU TERHADAP STAGING SPERMATOGENESIS DAN SEL LEYDIG PADA MENCIT (Mus musculus) YANG DIINFEKSI Toxoplasma gondii Fitri, Paraswita Eindah; Wurlina, Wurlina; Chusniati, Sri; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Plumeriastuti, Hani
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.345 KB) | DOI: 10.20473/ovz.v8i1.2019.10-16

Abstract

The aim of this research is to know about the staging of spermatogenesis and leydig cells in mice that injected by Toxoplasma gondii after giving honey as a preventive substance. This research used 25 mice in age 2-4 month with 20-30 weight. Mice were divided into 5 groups (P0, P1, P2, P3 dan P4). P0 as a control teratment were given aquades in peroral, P1 was a group given honey in doses I (0.08ml/0.2ml/day) during 14 days in peroral, P2 was a group that given infection by T.gondii in doses 103 in peritonial, P3 was given honey in doses I 0.08ml/0.2ml/day) then injected by T.gondii, and P4 was given honey in doses II (0.12ml/0.2ml/day) then injected by T.gondii. This research conducted in 18 days, in day 14th mice were given honey and the day 15th–18th mice injected by T.gondii doses 103. The data result was analyzed with Analysis of Variant (ANOVA) and cotinued with Duncan. Comparision statistical showed with SPSS statistic 20 for Windows. The result of analysis, P0 and P1 were significantly different (p<0.05), with the increase number of spermatogenic and leydig cells. P0 and P2 also significantly different, with the decrease number of spermatogenic and leydig cells. In P2 and P3 group were not significantly different (P>0.05), also in P4 group were not significantly different with P0 and P1. The results of this research showed that honey in doses II can maintain the number of sprematogenic and leydig cells as a preventive substance against T.gondii.
THE DILATATION OF BRAIN VENTRICLE DUE TO CONGENITAL TOXOPLASMOSIS IN MICE CORRELATED WITH APOPTOSIS BUT NOT WITH TRANSFORMING GROWTH FACTOR BETA Suwanti, Lucia Tri; Mufasirin, Mufasirin; Plumeriastuti, Hani
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.5428

Abstract

This study aimed to determine the occurences of mice brain ventricles dilatation that congenitally infected with Toxoplasma gondii (T. gondii) as a marker of hydrocephalus and cellular changes in the brain. A total of twenty pregnant mice (11.5 days pregnacy) were divided into 2 groups, which were control (P1) group and treatment (P2) group. The mice in the treatment group were infected with 101 tachyzoites of T. gondii. All mice were maintained until delivery. The newborn mice were sacrificed and their brain were removed and fixed in 10% buffered formalin to prepare histology slides with HE staining for observation of ventricular width, TUNEL assay for apoptosis observation, and immunohistochemistry for the expression of transforming growth factor beta (TGF-) observations. The data were analyzed using t test and linear regression. The results showed that ventricular width and apoptosis index significantly increased (P0.01) in the treatment group compared to control group, but there was no difference in the expression of TGF- (P0.05) in both groups. Dilatation of ventricle correlated with the apoptotic index of brain cells but did not correlated with the expression of TGF-.
PENINGKATAN TNF- DAN INDEKS APOPTOSIS PADA TULANG MENCIT YANG DIINFEKSI Toxoplasma gondii Suwanti, Lucia Tri; M, Mufasirin
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2808

Abstract

Penelitian ini bertujuan mengetahui peningkatan tumor necrosis factor-alpha (TNF-) dan indeks apoptosis pada tulang mencit yang diinfeksi Toxoplasma gondii (T. gondii). Tiga puluh dua ekor mencit dibagi menjadi dua kelompok. Kelompok 1 (K1), merupakan kelompok kontrol, tidak diinfeksi sedangkan Kelompok 2 (K2) diinfeksi dengan 10 takizoit T. gondii secara intraperitoneal. Enam hari setelah infeksi mencit dikorbankan, diambil tulang femur dan dilakukan pembuatan preparat histologis dengan pengecatan immunohistochemistry (IHC) dan Tunel assay. Hasil penelitian menunjukkan jumlah sel tulang yang mengekspresikan TNF- pada K2 (27,046,92) berbeda sangat nyata dibandingkan dengan K1 (11,423,92). Indeks apoptosis pada K1 dan K2 masing-masing adalah 9,173,04 dan 16,283,37. Dari hasil penelitian disimpulkan bahwa infeksi T. gondii meningkatkan TNF- dan indeks apoptosis sel tulang femur.
Analisis Molekuler Tilapia Lake Virus (TiLV) di Sentra Budidaya Benih Ikan Gurami (Osphronemus goramy) Jawa Timur Jumria, Andi; Amin, Muhamad; Mufasirin, Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9515

Abstract

Tilapia Lake Virus (TiLV) is a New Emerging Disease that has been defined and included in the WOAH disease list (WOAH, 2022). This virus can infect both freshwater and marine fish, causing high mortality in Ttilapia up to 90%. Reverse transcyptase method - Semi Nested Polymerase Chain Reaction (RT-snPCR) for detection of TiLV in Osphronemus goramy. TiLV was discovered for the first time in Thailand and was also found in Jogjakarta in 2021. Further research is needed to see the damage and genetic variation caused by TiLV in Osphronemus goramy. This study aims to study the genetic variability and homology of TiLV isolates in Osphronemus goramy as definitive hosts in East Java. The results showed clinical symptoms of lethargic fish, decreased appetite, abnormal behavior, reduced reflexes, sluggish movements, darker body color, congestion, thinning fins and exopthalmia. The PCR results showed that the Osphronemus goramy showing clinical symptoms of TiLV infection were all positive. The sequencing results were further identified using the BLAST program, showing the highest similarity of the genetic identity of the sample at 96.8% with TiLV from Israel (Genebank Accession Number NC 029927.1).
Kekerabatan Zeylanicobdella arugamensis pada Ikan Kerapu Cantang dengan Pendekatan Molekuler di Jawa Timur dan Lombok Ochtavia, Sherly; Mahasri, Gunanti; Mufasirin, Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10627

Abstract

Giant grouper is a highly sought-after cultivated fish in Indonesia due to its high market value. One of the challenges in grouper aquaculture is the infestation of the sea leech ektoparasite Zeylanicobdella arugamensis. The prevalence rate of ektoparasite infestation reaches up to 100%. An effective method to manage ektoparasite infestation is through molecular profiling, serving as preliminary literature in the development of molecular biology efforts to overcome cultivation challenges in giant grouper. This research aims to study the homology and relationship of Zeylanicobdella arugamensis on giant grouper in East Java, specifically in Lamongan, Mandangin, Situbondo, and Lombok. The method used is RT-PCR with mtDNA COI gene primers. Molecular sequence data were collected from samples and compared with data from NCBI GenBank, and analyzed using Neighbor-joining phylogenetic tree method and homology data analysis on BLAST. The research results indicate clinical symptoms in giant grouper including swelling, lesions/wounds, pale skin, decreased appetite, and fin erosion. Electrophoresis results from PCR products show a single band at 750bp for samples from Lamongan, Situbondo, Mandangin, and Lombok. DNA sequencing results on NCBI BLAST and homology analysis against the reference gene were conducted. Homology analysis results between samples from Situbondo and Lamongan show the highest similarity at 98.28%, whereas the lowest similarity is between samples from Mandangin and Situbondo at 88.51%. Based on phylogenetic tree analysis, samples from Lamongan and Situbondo waters show a close relationship with a genetic distance of 0.009272.
Prevalence and Clinical Characteristics of Human Hepatic Capillariasis in Asia: Review Choirunnisa, Indaka Rachmah; Suwanti, Lucia Tri; Mufasirin; Puspitasari, Heni
Media Kedokteran Hewan Vol. 37 No. 1 (2026): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v37i1.2026.98-113

Abstract

Human hepatic capillariasis in Asia is likely under-detected rather than rare. This review aimed to synthesize prevalence signals and describe clinical characteristics of hepatic capillariasis caused by Calodium (Capillaria) hepatica across Asian countries. A systematic search of major databases through 2025 identified case reports, case series, and hospital-based studies on human hepatic capillariasis. We extracted data on study design and diagnostic approaches, including histopathology, imaging, and molecular assays such as 18S rRNA sequencing. Clinical outcomes were recorded separately. Evidence was sparse and geographically clustered, with 20 publications from 7 countries concentrated in East and Southeast Asia. Most reports described single patients or small series. Clinical presentations commonly included hepatic mass-like lesions and eosinophilia, and misdiagnosis as neoplasia or other hepatobiliary disease was frequent. Histopathology was the primary diagnostic tool. Molecular confirmation was applied inconsistently, but, when used, enabled species-level identification. Because of heterogeneity in diagnostics (microscopy, histopathology, PCR), study populations (single cases vs hospital series), and locations (sites across East and Southeast Asia), and because denominators were small, we did not calculate a pooled prevalence. Nevertheless, high carriage rates in urban rodent reservoirs suggest under-recognition in humans. As part of integrated One Health surveillance, this review highlights the need to integrate histopathology with molecular testing, adopt standardized reporting that distinguishes genuine infections from spurious findings, and strengthen cross-sector collaborations to refine burden estimates and guide prevention across Asia.
Cestodiasis: A Narrative Review of Neglected Zoonotic Cestodes from Wild Rodents Nurmayani, Seli; Tri Suwanti, Lucia; Mufasirin
Journal of Parasite Science Vol. 10 No. 1 (2026): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v10i1.76600

Abstract

Zoonotic cestodes associated with wild rodents constitute an underrecognized component of neglected zoonotic diseases, particularly in resource-limited settings where ecological and sanitary conditions facilitate transmission. Although several rodent-borne cestodes, including Hymenolepis nana, Hymenolepis diminuta, Raillietina spp., and Inermicapsifer madagascariensis, are known to infect humans, their epidemiological significance remains insufficiently understood. This review synthesizes current evidence on the morphology, life cycles, host associations, clinical manifestations, and diagnostic challenges of zoonotic cestodes linked to wild rodents, with emphasis on their public health relevance. Available studies indicate that these infections are globally distributed but frequently underdiagnosed and underreported. Their predominantly asymptomatic nature, coupled with nonspecific clinical presentations and diagnostic limitations, contributes substantially to their neglected status. Children appear disproportionately affected, likely due to behavioral and hygiene-related factors that increase exposure risk. Rodents serve as important reservoirs, with numerous investigations documenting zoonotic cestode infections in peri-domestic and urban rodent populations. However, significant knowledge gaps persist regarding species diversity, geographical distribution, and transmission dynamics between rodents and humans. From a public health perspective, the overlooked nature of rodent-associated cestode infections highlights the need for improved surveillance, strengthened diagnostic capacity, and greater clinical awareness. Preventive strategies should prioritize sanitation, food safety, and health education, particularly in vulnerable populations. Addressing these gaps is essential for enhancing understanding of the epidemiology of zoonotic cestodes and for developing effective control and prevention measures.
Molecular Identification and Phylogenetic Reconstruction of Horseshoe Crab (Xiphosura, Limulidae) from the East Java Region Sapto Andriyono; Catlya Tasya Adella Tasya Adella; Mufasirin Mufasirin; Lailatul Lutfiyah; Heru Pramono; Nur Izzati Mohd Noh; Dwi Anggorowati Rahayu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 31, No 2 (2026): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.31.2.181-194

Abstract

Horseshoe crabs, ancient marine arthropods of the Limulidae family, are currently facing significant threats due to overexploitation driven by high demand for their eggs, which are widely consumed, and their hemolymph, which is critical for biomedical applications. This study aims to evaluate the molecular identification and phylogenetic placement of horseshoe crabs in East Java, a region previously unreported in this context. DNA barcoding was employed to analyze the mitochondrial COI gene region, complemented by detailed morphological identification, to provide a comprehensive understanding of the species in this area. A universal primer LCO/HCO was used for PCR, as described in a previous report, and Sanger sequencing was performed to confirm the approximately 655 bp amplification product. This research identifies horseshoe crabs from six distinct locations in East Java as Tachypleus gigas using molecular methods, with genetic analysis indicating>99% similarity to T. gigas sequences in the GenBank database. Morphological examinations of specimens from seven areas further corroborated the presence of T. gigas, distinguished by characteristic serrations along the telson and a single spine on the posterior region of the opisthosoma. The phylogenetic tree, constructed from our genetic data, shows that Tachypleus gigas populations in East Java are clustered in the same clade as those in Southeast Asia, as supported by GenBank records. This study underscores the importance of integrating genetic and morphological approaches for accurate species identification. Moreover, it provides crucial baseline data for developing conservation strategies to preserve Tachypleus gigas populations in East Java, thereby contributing to broader conservation efforts for this species.
Co-Authors Abdul Samik Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Adikara, Tatang Santanu Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Al Arif, Muhammad Anam Alasrorik, Muhammad Hizbulloh Alfina Azkiana Amalia Rosydinasari Rosydinasari Anam Al Arif Andi Jayawardhana andi jayawardhana Ardianto Ardianto Arif Pratiwi Arif Rahman Nurdianto Arimbi Aryaloka, Suhita Aryati Aryati Aryati Aryati Azizah Bilqis Nurkarimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Benjamin Christoffel Tehupuring, Benjamin Christoffel Berlina, Cyrcilia Relita Boedi Setiawan Catlya Tasya Adella Tasya Adella Choirunnisa, Indaka Rachmah Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Desty Shafira Dewi Purwatiningsih Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Djoko Legowo Djoko Legowo Doohan Mahendra Doohan Mahendra Dwi Anggorowati Rahayu DWI PUTRI RAHMAWATI Dyah Ayu Kurniawati Dyah Ayu Kurniawati Edward Yonas Kristijanto Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Elok Apriliawati Emy Koestanti Sabdoningrum Endah Rochmatika Endang Suprihati Epy Muhammad Luqman Erma Safitri Fakhryyah Maharani Deviyanti Farezi, Reza Adrio Fatmawati, Mira Fatmawati Fauziah Fitri Hernanto Fedik Abdul Rantam Firdaus , Muhammad Aviv Fitri, Paraswita Eindah Fransiska Cicilia Beka Frida Aulya Arningdiah Galaxy Guardian Gunanti Mahasri Haditanojo, Wiyanto Hana Eliyani Hana Eliyani Hani Plumeriastuti Hanna Harnida, Hanna Hartono Hartono Hayuning Nurrodhiya Heni Puspitasari Herdiansyah, Akbar Dimas Hermin Ratnani Herry Agoes Hermadi Heru Pramono Hidanah , Sri Hidanah, Sri Ihda Hanny, Khurun'In Fadia IMAM MUSTOFA Indasari, Elly Nur Ira Sari Yudaniayanti Istiana, Izzatul Iwan Sahrial Hamid Jayanti Dian Eka Sari, Jayanti Dian Joko Prastowo Jumria, Andi Kenconojati, Hapsari Khairullah, Aswin Rafif Khalissa Farah Alifia Koesdarto Koesdarto Kurniawan, Muhammad 'Ahdi Kurniawan, Muhammad ‘Ahdi Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lailatul Lutfiyah, Lailatul Legowo, Djoko Lestari Wilujeng Lilik Maslachah Lisnanti, Ertika Fitri Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Luthfiyyah Nur Afifah Siswandi Mafruchati, Maslichah Maghfiroh, Nurutin Tutur Bifatikhatil M Masdiana C Padaga Melani Anggraini Melanie Aulia Ashfiyah Mirni Lamid Mirni Lamid Mochammad Amin Alamsjah Mohamad Yusuf Mohammad Guritno Suryokusumo Moses, Ikechukwu Benjamin Mufa, Ramy Inas Mahirah Muhamad Amin Muhammad Ahdi Kurniawan Muhammad Al-Syafiq bin Abdul Halim Muhammad Ridwan Muslimah, Bintang Mustofa Helmi Effendi Nedi Nedi Nenny Harijani Ni Komang Aprilina Widisuputri Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nur Izzati Mohd Noh Nurdianti Nurdianti Nurdianto, Arif Rahman Nurfaizah, Diza Ulya Nurmayani, Seli Nusdianto Triakoso Nuurin Ajrin Karim Ochtavia, Sherly P Kumaladewi Permata Sari, Dian Ayu Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastoetik Poedji Hastutiek Poetranto, Emmanuel Djoko Pradana, Giffari Danindra Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Pratiwi, Arif Primarizky, Hardany Puput Ade Wahyuningtyas Puput, Sesa Purbowati, Tri Endah Puspikawati, Septa Indra Rahadju Ernawati Rahardjo, Adi Prijo Rahmandari, Dina Agylia Rahmatullah, Aldin Akbar Ramadhana Ramadhana Ramadhanty, Miladhiyah Nabila Ratna Damayanti Rekasni Adallin A/P Morgan A/P Morgan Retno Yuli Rimayanti Rina Vitriasari Riwu, Katty Hendriana Priscilia Romy Muhammad Dary Mufa Romziah Sidik Rosyta, Pegy Saksono, Bayu Sapto Andriyono Sari, Fifin Kurnia Septian Hakim Susantoputro Setiawan Koesdarto Setiawan Kusdarto Sherly Ochtavia Silfia, Himatul Ilma Soeharsono Soeharsono Soetojo Soetojo Sri Agus Sudjarwo Sri Pantja Madyawati Sukarno, Gerda Sumartono Sumartono Sunarso, Agus Susilo, Rahadian Indarto Talita Yuanda Reksa Taufan Ary Handoko Tita Damayanti Lestari Tony Hartono Tri Wahyu Suprayogi Trifena Pristi Anindyta Tyasningsih, Wiwiek Vindo Rossy Pertiwi Wahidan Qodiip Maulana Warda Nafalizza Efendi Warsito, Sunaryo Hadi Widi Nugroho Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Win Darmanto Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wurlina, W Yudanianyi, Ira Sari Yudaniayanti , Irasari Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad ZULFAH