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Seroprevalensi Kasus Toxoplasmosis sebagai Infeksi Oportunistik pada Penderita HIV/ Acquired Immune Deficiency Syndrome (AIDS) Di Kabupaten Sidoarjo Andi Jayawardhana; Harnida, Hanna; Dewi Purwatiningsih; Heni Puspitasari; Mufasirin; Lucia Tri Suwanti; Arif Rahman Nurdianto
NERSMID : Jurnal Keperawatan dan Kebidanan Vol. 6 No. 1 (2023): Mei
Publisher :

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55173/nersmid.v6i1.133

Abstract

Toxoplasma gondii is an obligate intracellular parasite that can cause toxoplasmosis infection. About 60% of the world's population has been infected. Toxoplasma cerebri is one of the most common opportunistic infections in the central nervous system of HIV patients. The disease is acute and chronic, with mild symptoms that are non-specific so it is difficult to distinguish from other diseases or is often asymptomatic. The incidence of Human Immunodeficiency Virus (HIV) occupies the highest place in Asia with high morbidity and mortality. Toxoplasma is ranked in the top 10 opportunistic diseases as a direct cause of morbidity and mortality, with variable symptoms and reactivation. The purpose of the study: to detect Toxoplasma gondii from blood samples of patients with HIV/Acquired Immune Deficiency Syndrome (AIDS). In immunocompromised conditions, especially HIV/AIDS, symptoms of severe complications will arise, one of which is toxsoplasma encephalitis (TE). The high rate of morbidity and mortality of TE in HIV/AIDS patients, around 30-40%, makes an accurate diagnosis important. Research Methods: The study design used was cross sectional to determine the level of IgG Toxoplasma Gondii antibodies in HIV patients and serologically. The research subjects were 30 individual subjects who were positive for HIV infection, of which 30 patients would be screened for the levels of IgG Toxoplasma Gondii antibodies and then see the correlation with lifestyle and food consumed. The achievement target of this research is to find out whether HIV patients have levels of IgG Toxoplasma Gondii antibodies through serological tests, so that appropriate therapy and education can be given for the treatment of toxoplasmosis, so that these results can be published scientifically in national journals, and become paper material in scientific meetings.
Prevalensi Parasit Saluran Pencernaan pada Kerbau (Bubalus bubalis) Melalui Pemeriksaan Feses di Kabupaten Agam Sumatra Barat Ridwan, Muhammad; Suwanti, Lucia Tri; Suprayogi, Tri Wahyu; Mufasirin, Mufasirin; Kusnoto, Kusnoto; Hastutiek, Poedji
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.607 KB) | DOI: 10.33772/jitro.v9i1.19763

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ABSTRAKPenelitian ini bertujuan untuk mengetahui jenis, prevalensi, dan pengaruh jenis kelamin serta umur terhadap prevalensi parasit saluran pencernaan pada kerbau di Kabupaten Agam Provinsi Sumatra Barat. Sampel yang digunakan dalam penelitian ini adalah feses dari 105 ekor kerbau yang dikumpulkan dari tiga Kecamatan. Pemeriksaan sampel dilakukan dengan menggunakan metode natif, sedimentasi, dan pengapungan. Penelitian ini memberikan hasil bahwa ditemukan infeksi saluran pencernaan pada kerbau berupa cacing Paramphistomum sp. 31,43%, Fasciola sp. 23,81%, Oesophagustomum sp. 1,9%, Bunostomum sp. 0,95%, Strongyloides sp. 0,95%, Trichostrongylus sp. 0,95%, dan protozoa Eimeria sp. 2,86%. Prevalensi yang diperoleh sebesar 45,7% (48/105). Hasil analisis statistik menunjukkan bahwa jenis kelamin dan umur tidak mempengaruhi prevalensi parasit saluran pencernaan pada kerbau di Kabupaten Agam Sumatra Barat.Keywords: cacing dan protozoa, kerbau, prevalensi, saluran pencernaanPrevalence of Gastrointestinal Parasite in Buffalo (Bubalus bubalis) Through Feces Examination in Agam Regency West SumatraABSTRACTThis study aims to determine the type, prevalence, and influence of sex and age on the prevalence of gastrointestinal parasites on buffalo in the Agam Regency of West Sumatra Province. The samples used in this study were feces from 105 buffaloes collected from three Districts. Examination of the sample is carried out using by direct smear methods, sedimentation, and flotation. This study provides results that found gastrointestinal infections in buffalo by helminth Paramphistomum sp. 31.43%, Fasciola sp. 23.81%, Oesophagustomum sp. 1.9%, Bunostomum sp. 0.95%, Strongyloides sp. 0.95%, Trichostrongylus sp. 0.95%, and protozoa Eimeria sp. 2,86%. Prevalence is 45.7% (48/105). The results of statistical analysis showed that sex and age did not affect the prevalence of gastrointestinal parasites on buffalo in the Agam Regency of West Sumatra.Keywords: buffalo, gastrointestinal, helmint and protozoa, prevalence
Analisis Molekuler Tilapia Lake Virus (TiLV) di Sentra Budidaya Benih Ikan Gurami (Osphronemus goramy) Jawa Timur Jumria, Andi; Amin, Muhamad; Mufasirin, Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9515

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Tilapia Lake Virus (TiLV) is a New Emerging Disease that has been defined and included in the WOAH disease list (WOAH, 2022). This virus can infect both freshwater and marine fish, causing high mortality in Ttilapia up to 90%. Reverse transcyptase method - Semi Nested Polymerase Chain Reaction (RT-snPCR) for detection of TiLV in Osphronemus goramy. TiLV was discovered for the first time in Thailand and was also found in Jogjakarta in 2021. Further research is needed to see the damage and genetic variation caused by TiLV in Osphronemus goramy. This study aims to study the genetic variability and homology of TiLV isolates in Osphronemus goramy as definitive hosts in East Java. The results showed clinical symptoms of lethargic fish, decreased appetite, abnormal behavior, reduced reflexes, sluggish movements, darker body color, congestion, thinning fins and exopthalmia. The PCR results showed that the Osphronemus goramy showing clinical symptoms of TiLV infection were all positive. The sequencing results were further identified using the BLAST program, showing the highest similarity of the genetic identity of the sample at 96.8% with TiLV from Israel (Genebank Accession Number NC 029927.1).
Kekerabatan Zeylanicobdella arugamensis pada Ikan Kerapu Cantang dengan Pendekatan Molekuler di Jawa Timur dan Lombok Sherly Ochtavia; Gunanti Mahasri; Mufasirin Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10627

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Giant grouper is a highly sought-after cultivated fish in Indonesia due to its high market value. One of the challenges in grouper aquaculture is the infestation of the sea leech ektoparasite Zeylanicobdella arugamensis. The prevalence rate of ektoparasite infestation reaches up to 100%. An effective method to manage ektoparasite infestation is through molecular profiling, serving as preliminary literature in the development of molecular biology efforts to overcome cultivation challenges in giant grouper. This research aims to study the homology and relationship of Zeylanicobdella arugamensis on giant grouper in East Java, specifically in Lamongan, Mandangin, Situbondo, and Lombok. The method used is RT-PCR with mtDNA COI gene primers. Molecular sequence data were collected from samples and compared with data from NCBI GenBank, and analyzed using Neighbor-joining phylogenetic tree method and homology data analysis on BLAST. The research results indicate clinical symptoms in giant grouper including swelling, lesions/wounds, pale skin, decreased appetite, and fin erosion. Electrophoresis results from PCR products show a single band at 750bp for samples from Lamongan, Situbondo, Mandangin, and Lombok. DNA sequencing results on NCBI BLAST and homology analysis against the reference gene were conducted. Homology analysis results between samples from Situbondo and Lamongan show the highest similarity at 98.28%, whereas the lowest similarity is between samples from Mandangin and Situbondo at 88.51%. Based on phylogenetic tree analysis, samples from Lamongan and Situbondo waters show a close relationship with a genetic distance of 0.009272.
Pengaruh Acidifier Terhadap Performa Produksi Dan Analisis Usaha Pada Ayam Pedaging Yang Diinfeksi Avian Pathogenic Escherichia coli (APEC) : The Effect of Acidifier on Production Performance and Business Analysis of Broiler Chickens Infected with Avian Pathogenic Escherichia coli (APEC) Silfia, Himatul Ilma; Warsito , Sunaryo Hadi; Mufasirin; Hidanah , Sri; Lokapirnasari, Widya Paramita; Yudaniayanti , Irasari; Firdaus , Muhammad Aviv; Lisnanti, Ertika Fitri
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 22 No. 3 (2024): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.22.3.144-150

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This study aimed to provide the impact of acidifier supplementation on feed consumption, body weight gain (BWG), feed efficiency, and feed conversion ratio (FCR) in broiler chickens infected with Avian Pathogenic Escherichia coli (APEC). The use of acidifiers is proposed as a potential solution to improve gut health and nutrient absorption. This study employed a completely randomized design (CRD). Forty broiler chickens, ages 1 to 35, were utilized in this study, and the treatment started at 8 days of age. There were 5 treatments and 4 replications for each treatment, with 2 broiler chicks in each replication. The treatments consisted of negative control, without APEC infection (P0), positive control, with APEC infection (P1), infected with APEC with 0.1% of Antibiotic Growth Promoters in the diet (P2), and infected with APEC with additional feed acidifier 0.5% (P3). This study analyzed its effect on feed consumption, Body Weight Gain (BWG), Feed Conversion Ratio (FCR), and feed efficiency. Also, the business analysis was carried out by calculating the contribution margin (CM). Data was statistically analyzed using one way-analysis of variance (ANOVA), followed by Duncan's multiple range test to determine any statistical differences (p<0.05). The research results show that adding an acidifier to the diet does not reduce the feed consumption value but can increase BWG, reduce the FCR value (p<0.05), improve feed efficiency, and increase the contribution margin in broiler chicken farming. It concludes that adding 0.5% acidifier to the feed can improve broiler chickens infected with APEC production performance.
Limousin cattle production performance and farmer income post-FMD fed with super booster and immunobooster concentrate Maghfiroh, Nurutin Tutur Bifatikhatil M; Al Arif, Mohammad Anam; Lamid, Mirni; Mustofa, Imam; Mufasirin, Mufasirin; Rahardjo, Dadik
Jurnal Ilmu Peternakan Terapan Vol 8 No 2 (2025): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v8i2.5795

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This study aims to evaluate the administration of super booster, and immunobooster concentrates on production performance and farmers' income following an outbreak of foot and mouth disease (FMD) by proving the improvements in production performance, including feed consumption, weight gain, and revenue of crossbred Limousin cattle farmers. This study used 18 male Limousin crossbred cattle selected based on age (1.5-2 years) and having been infected with FMD virus that is divided into three treatments: T0 (control feed consisting of forage and local concentrate), T1 (control feed + 40 g of a super booster), and T2 (control feed + 600 g of immunobooster concentrate). Data collection and observation were conducted over 42 days. Statistical analysis was performed using analysis of variance (ANOVA) based on the completely randomized design (CRD), followed by Duncan’s multiple range test (DMRT). The results of the variance analysis showed a p-value of <0.05, indicating that the addition of super booster and immunobooster can improve feed consumption, body weight gain, and increase farmers income in crossbred Limousin cattle farming following an outbreak of FMD, so that it can be used as an alternative step in overcoming post-FMD losses and helping farmers maximize profits.
Toxoplasma Oocyst Contamination in Water Sources in Goat-Sheep Farming In Sidoarjo District Mufa, Ramy Inas Mahirah; Eka Pramyrtha Hestianah; Lucia Tri Suwanti; Hani Plumeriastuti; Widya Paramita Lokapirnasari; Tony Hartono; Rina Vitriasari; Retno Yuli; Galaxy Guardian; Heni Puspitasari; Mufasirin
Journal of Parasite Science Vol. 9 No. 1 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i1.67669

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Toxoplasmosis is one of the zoonotic diseases caused by the protozoa Toxoplasma gondii. The disease infects warm-blooded animals including humans. The purpose of this study was to determine the contamination of Toxoplasma oocysts in water sources in goat-sheep farms in Sidoarjo Regency. A total of 63 water samples were used for the study. Water samples were taken from 5 sub-districts that have many goat-sheep farms, each sub-district has 2 villages, with each village having 6-7 samples. The examination used the floating method and was examined under a microscope with a magnification of 400x. The results of the examination found 21 samples of Toxoplasma gondii oocysts or 33.33% (21/63). The morphology of T.gondii oocysts is round, ovoid or ellipsoid with sizes varying between 8.02 - 12.72 micrometers. Preventive measures are needed by treating water before giving it to livestock.
Detection of Protozoa Intestinal Zoonotic in Madura Cattle in Bangkalan Madura Regency Sunarso, Agus; Poedji Hastutiek; Mufasirin
Journal of Parasite Science Vol. 9 No. 1 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i1.69429

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The study aimed to detect the protozoan intestinal zoonotic that can potentially be a strategic disease in Madura cattle. The prevalence of the disease was linked to the sex and age of cattle in Bangkalan Regency. Cattle feces samples were collected 400 samples from eight sub-districts in Bangkalan District. Feces were examined natively, sedimentation, and floating. The species of protozoa was determined based on its morphology. The prevalence was expressed in percent, the study results were presented in figures and tables. The prevalence of protozoan intestinal zoonoses was 27.75%. The highest rate was found in Kwanyar (50%). Four species, namely Blastocystis hominis, Balantidium coli, Entamoeba coli, and Giardia bovis. In a single infection, B. hominis as the most protozoa was found to infect 85 cattle in eight sub-districts, B. coli was found in 16 cattle in four sub-districts and E. coli in 5 cattle in Kwanyar. A total of 107 samples were found to be infected with a single protozoan species, while 4 samples contained two species. The infection rate in bulls was 26.66%, which was slightly lower than in cows at 28.06%. Protozoan infections were more common in cattle aged 6 months to 2 years (42.4%) and those under 6 months (35.8%) compared to cattle over 2 years old (16.4%). This study confirmed the presence of intestinal zoonotic protozoa in cattle in Bangkalan District. Further research is needed to assess the prevalence of protozoa, identify risk factors, and analyze their geographic distribution, for developing effective prevention and control strategies.
Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia Kurniawan, Muhammad 'Ahdi; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Suprihati, Endang; Hastutiek, Poedji; Kusnoto, Kusnoto; Ansori, Arif Nur Muhammad; Puspitasari, Yulianna; Khairullah, Aswin Rafif; Moses, Ikechukwu Benjamin; Pratama, Bima Putra; Riwu, Katty Hendriana Priscilia
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.153-166

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.
ANALYSIS OF REPRODUCTIVITY OF MADRASIN CATTLE BEFORE AND AFTER FOOT AND MOUTH DISEASE ON GILI IYANG ISLAND, SUMENEP, MADURA Zulfah; Sabdoningrum, Emy Koestanti; Hidanah, Sri; Lokapirnasari, Widya Paramita; Mufasirin; Yudanianyi, Ira Sari
Jurnal Biosains Pascasarjana Vol. 27 No. 1 (2025): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v27i1.2025.32-35

Abstract

This research aims to analyze reproductivity (S/C and Pregnancy) and supply and demand for Madrasin cattle before and after FMD disease on Gili Iyang Island, Sumenep, Madura. This research used data from 450 madrasin cattle on Gili Iyang Island, Sumenep. The research was carried out by comparing data obtained before the FMD outbreak in January 2022 – May 2022 and after the FMD outbreak in June 2022 – November 2022. The results of the research showed that the reproductivity of Madrasin cattle before and after the FMD disease on Gili Iyang Island, Sumenep, Madura in S/C increased, while during pregnancy there is a decrease. The supply and demand for the Madrasin cattle business before and after the FMD on Gili Iyang Island, Sumenep, Madura, decreased.
Co-Authors Abdul Samik Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Adikara, Tatang Santanu Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Al Arif, Muhammad Anam Alasrorik, Muhammad Hizbulloh Alfina Azkiana Amalia Rosydinasari Rosydinasari Anam Al Arif Andi Jayawardhana andi jayawardhana Ardianto Ardianto Arif Pratiwi Arif Rahman Nurdianto Arimbi Aryaloka, Suhita Aryati Aryati Aryati Aryati Azizah Bilqis Nurkarimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Benjamin Christoffel Tehupuring, Benjamin Christoffel Berlina, Cyrcilia Relita Boedi Setiawan Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Desty Shafira Dewi Purwatiningsih Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Djoko Legowo Djoko Legowo Doohan Mahendra Doohan Mahendra DWI PUTRI RAHMAWATI Dyah Ayu Kurniawati Dyah Ayu Kurniawati Edward Yonas Kristijanto Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Elok Apriliawati Emy Koestanti Sabdoningrum Endah Rochmatika Endang Suprihati Endang Suprihati Erma Safitri Fakhryyah Maharani Deviyanti Farezi, Reza Adrio Fatmawati, Mira Fatmawati Fauziah Fitri Hernanto Fedik Abdul Rantam Firdaus , Muhammad Aviv Fitri, Paraswita Eindah Fransiska Cicilia Beka Frida Aulya Arningdiah Galaxy Guardian Gunanti Mahasri Haditanojo, Wiyanto Hana Eliyani Hana Eliyani Hani Plumeriastuti Hanna Harnida, Hanna Hartono Hartono Hayuning Nurrodhiya Heni Puspitasari Herdiansyah, Akbar Dimas Hermin Ratnani Herry Agoes Hermadi Hidanah , Sri Hidanah, Sri Ihda Hanny, Khurun'In Fadia IMAM MUSTOFA Indasari, Elly Nur Ira Sari Yudaniayanti Istiana, Izzatul Iwan Sahrial Hamid Jayanti Dian Eka Sari, Jayanti Dian Joko Prastowo Jumria, Andi Kenconojati, Hapsari Khairullah, Aswin Rafif Khalissa Farah Alifia Koesdarto Koesdarto Kurniawan, Muhammad 'Ahdi Kurniawan, Muhammad ‘Ahdi Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lestari Wilujeng Lilik Maslachah Lisnanti, Ertika Fitri Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Lutfiah Annisa Billa Luthfiyyah Nur Afifah Siswandi Mafruchati, Maslichah Maghfiroh, Nurutin Tutur Bifatikhatil M Masdiana C Padaga Melani Anggraini Melanie Aulia Ashfiyah Mirni Lamid Mirni Lamid Mochammad Amin Alamsjah Mohamad Yusuf Mohammad Guritno Suryokusumo Moses, Ikechukwu Benjamin Mufa, Ramy Inas Mahirah Muhamad Amin Muhammad Ahdi Kurniawan Muhammad Al-Syafiq bin Abdul Halim Muhammad Ridwan Muslimah, Bintang Mustofa Helmi Effendi Nedi Nedi Nenny Harijani Ni Komang Aprilina Widisuputri Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nurdianti Nurdianti Nurdianto, Arif Rahman Nurfaizah, Diza Ulya Nusdianto Triakoso Nuurin Ajrin Karim Ochtavia, Sherly P Kumaladewi Permata Sari, Dian Ayu Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastoetik Poedji Hastutiek Poetranto, Emmanuel Djoko Pradana, Giffari Danindra Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Pratiwi, Arif Primarizky, Hardany Puput Ade Wahyuningtyas Puput, Sesa Purbowati, Tri Endah Puspikawati, Septa Indra Rahadju Ernawati Rahardjo, Adi Prijo Rahmandari, Dina Agylia Rahmatullah, Aldin Akbar Ramadhana Ramadhana Ramadhanty, Miladhiyah Nabila Ratna Damayanti Rekasni Adallin A/P Morgan A/P Morgan Retno Yuli Rimayanti Rina Vitriasari Riwu, Katty Hendriana Priscilia Romy Muhammad Dary Mufa Romziah Sidik Rosyta, Pegy Sabdoningrum, Emy Koestanti Saksono, Bayu Sapto Andriyono Sari, Fifin Kurnia Septian Hakim Susantoputro Setiawan Koesdarto Setiawan Kusdarto Sherly Ochtavia Silfia, Himatul Ilma Soeharsono Soeharsono Soetojo Soetojo Sri Agus Sudjarwo Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Sukarno, Gerda Sumartono Sumartono Sunarso, Agus Susilo, Rahadian Indarto Talita Yuanda Reksa Taufan Ary Handoko Tita Damayanti Lestari Tony Hartono Tri Suwanti, Lucia Tri Wahyu Suprayogi Trifena Pristi Anindyta Tyasningsih, Wiwiek Vindo Rossy Pertiwi Wahidan Qodiip Maulana Warda Nafalizza Efendi Warsito, Sunaryo Hadi Widi Nugroho Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Win Darmanto Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wurlina, W Yudanianyi, Ira Sari Yudaniayanti , Irasari Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad ZULFAH