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All Journal ACTA VETERINARIA INDONESIANA Media Kedokteran Hewan Folia Medica Indonesiana Briliant: Jurnal Riset dan Konseptual Caraka Tani: Journal of Sustainable Agriculture Jurnal Medik Veteriner Jurnal Ilmiah Fillia Cendekia Jurnal Ilmu Peternakan Terapan Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Bantara Journal of Animal Science Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat LOSARI: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Innovation Studies Journal of Fisheries & Marine Jurnal Agro Veteriner
Al arif, Mohammad Anam
Departemen Peternakan, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C UNAIR Mulyorejo, Surabaya, Jawa Timur, Indonesia 60115
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Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches Rozi; Tyasningsih, Wiwiek; Rahmahani, Jola; Aksono, Eduardus Bimo; Yunus , Muchammad; Al-Arif, Mohammad Anam; Kuncorojati, Suryo; Kusdarwati, Rahayu; Sari, Putri Desi Wulan; Amal, Mohammad Noor Azmai; Salleh, Annas; Khanand, Nadeem; Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.62035

Abstract

Graphical Abstract Highlight Research The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity. In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity. Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation. The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines. Abstract Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate.  
PENGARUH pH LENDIR MUKOSA VAGINA SAAT BIRAHI TERHADAP PERSENTASE KEBUNTINGAN (Conception Rate) PADA SAPI PERAH DI KUD TANI WILIS KABUPATEN TULUNGAGUNG DAN KSU TUNAS SETIA BARU KABUPATEN PASURUAN Rizki, Andaru; Srianto, Pudji; Suprihati, Endang; Sardjito, Trilas; Ismudiono, Ismudiono; Al Arif, Mohammad Anam
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.312 KB) | DOI: 10.20473/ovz.v8i2.2019.154-158

Abstract

The purpose of this research is to determine the effect of mucos acidity of vaginal mucous when estrus towards conception rate at KUD Tani Wilis Tulungagung District and KSU Tunas Setia Baru Pasuruan District. Measured samples were taken from 30 dairy cattles in each KUD. Mucos acidity of vaginal mucous were obtained by using a digital acidity meter before insemination was done on dairy cattle. After measuring the acidity from mucosal vagina mucous, artificial insemination has done on estrus dairy cattle. On the 60th day after insemination, rectal palpation was done to check the conception rate. Research result from 30 dairy cattle on each KUD showed that mucos acidity of vaginal mucous has signifficant effect towards conception rate. Mucos acidity of vaginal mucous range 6.5-8.5 with the highest range of conception rate in KSU Tunas Setia Baru Pasuruan District 7.5-7.7 and in KUD Tani Wilis Tulungagung District with the highest range of conception range 7.2-7.4 and 7.5-7.7.
Supplementation with mineral mix or concentrate booster restored fertility in cows following foot-and-mouth disease infection Aris Puji Nugroho; Ali Agus; Moh. Sofiul Anam; Suherni Susilowati; Herry Agoes Hermadi; Dian Ayu Permatasari; Mohammad Anam Al Arif
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.127-137

Abstract

This study aimed to evaluate the effects of supplementing mineral mix and concentrate booster on the fertility of cows recovering from foot-and-mouth disease (FMD). Eighteen post-FMD cows were assigned to three treatment groups. Cows in the T0 group received a standard feed consisting of elephant grass and a local concentrate. In the T1 group, cows were provided the standard feed supplemented with 40 g/day of mineral mix, while cows in the T2 group received the standard feed supplemented with 600 g/day of concentrate booster. The feeding treatments were applied for 42 days, and fresh drinking water was provided ad libitum. The results showed that the onset of estrus occurred significantly earlier (p <0.05) compared to the anestrus period experienced during FMD infection. Both supplemented groups exhibited higher (p <0.05) estrus rates, pregnancy rates, and calving rates than the T0 group. The interval between the last estrus and the initiation of additional feeding was considerably longer than the onset of estrus observed after treatment. The pH and ferning scores of cervical mucus in cows from the T0 group were lower than those in the T1 and T2 groups. However, no significant differences (p >0.05) were found in cervical mucus pH or ferning scores between the T1 and T2 groups, between pregnant and non-pregnant cows, or between offspring sex categories. In conclusion, supplementation with either a mineral mix or a concentrate booster improved fertility parameter, including estrus rate, pregnancy rate, and calving rate, in cows recovering from FMD.
Polyvalent formalin-killed Aeromonas hydrophila vaccine with oil adjuvant drives coordinated humoral, innate, and cytokine responses in giant gourami (Osphronemus goramy) Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan 2026: JIPK VOLUME 18 ISSUE 1 YEAR 2026 (FEBRUARY 2026, ISSUE IN PROGRESS)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Graphical Abstract  Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture.   Abstract Bacterial septicaemia caused by Aeromonas hydrophila is an important constraint for giant gourami (Osphronemus goramy) culture in Southeast Asia. Inactivated whole-cell (bacterin) vaccines are widely used against bacterial diseases in aquaculture, but comparative data on monovalent versus polyvalent A. hydrophila vaccines, with and without oil-based adjuvant, remain scarce for this species. This study evaluated the safety, immunological responses, and protective efficacy of three formalin-killed cell (FKC) vaccines prepared from gourami-derived A. hydrophila isolates: a monovalent FKC (P2), a non-adjuvanted polyvalent FKC (P3), and an oil-adjuvanted polyvalent FKC (P4), using PBS (P1) as a control. Sub-adult giant gourami were vaccinated intraperitoneally and monitored for 42 days. Serum agglutinating antibody titres, nitroblue tetrazolium (NBT)-reducing activity, and splenic il-1β and ifn-γ mRNA expression were measured at multiple time points. At 21 days post-vaccination, a separate cohort was challenged intraperitoneally with virulent A. hydrophila Ah-S1, and survival was recorded for 14 days; relative percent survival (RPS) was calculated at day 14. All FKC formulations were clinically well tolerated, with only transient post-vaccination inappetence and no gross injection-site pathology. Vaccination induced clear, treatment- and time-dependent increases in agglutinating antibody titres, NBT-reducing activity, and splenic il-1β and ifn-γ transcription, with the strongest and most sustained responses in P4, intermediate responses in P3 and P2, and minimal changes in P1. Following homologous challenge, day-14 survival was 8.3% in P1, 61.7% in P2, 75.0% in P3, and 83.3% in P4, with corresponding RPS values of 58.2%, 72.7%, and 81.8%, respectively. Under these experimental conditions, the oil-adjuvanted polyvalent FKC (P4) produced the greatest enhancement of immune responses and protection against intraperitoneal A. hydrophila challenge in giant gourami. These findings support this formulation as a candidate for further vaccine development in O. goramy and highlight the need for dose optimisation, safety assessment, heterologous challenge, and field-validation studies before recommendations for large-scale use in aquaculture.
Co-Authors Agung Prasetyo Agustono, Bodhi Aksono HP., Eduardus Bimo Alfanindya, Evita Aisyah Ali Agus Amal, Mohammad Noor Azmai Amung Logam Saputro Anastasya, Jelita Andreas Berny Yulianto, Andreas Berny Andriani, Asafarid Annas Salleh Annisa Nur Aulia Purwantoro Ardianto Ardianto Aris Puji Nugroho Aryaloka, Suhita Ayuti, Siti Rani Balgis Al Basyarahil Bambang Prajogo E.W Cantika Putri Melyana Caska - Chandra, Evania Haris Chusniati, Sri Dadik Rahardjo Dadik Rahardjo, Dadik Daruti Dinda Nindarwi Devi Sri Rohmani Dewi, Nina Nurmalia Dian Ayu Permatasari Dimas Kunto Satrio Dita Wisudyawati Djoko Legowo Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri ERMA SAFITRI Faiqoh, Berliana Elok Faisal Fikri Fatin Fadhilah Hasib Fauzia, Kartika Afrida Fauziah, Ima Fuddin, Muhammad Nazar Gandul Atik Yuliani Gazali Salim Gesa Cantya Primatara Hadiah Fitriyah Hani Plumeriastuti Hapsari, Tiara Harijani, Nenny Hasib, Abdullah Hendarti, Gracia Angelina HERAWATI, LILIK Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Hussain, Muhammad Asif IMAM MUSTOFA Iqbal Laksana Ira Sari Yudaniayanti Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Joel Jeevan Raj S/O Yogarajah Kadek Rachmawati Khairullah, Aswin Rafif Khanand, Nadeem Kirana, Amelia Lintang Putri Koesnoto Soepranianondo Koesnoto Supranianondo Kuncorojakti, Suryo Kuncorojati, Suryo Kurniasih, Dea Anita Ariani Kusala, Muhammad Khaliim Jati Lilik Maslachah Lovela, Aprinda Ratna Ma'ruf, Muchammad Achsinul Fikri Mafruchati, Maslichah Maghfiroh, Nurutin Tutur Bifatikhatil M Miarsono, Sigit Mirni Lamid Mirni Lamid Mochamad Lazuardi Moh. Sofiul Anam Moh. Zaky ‘Ubaidillah Mohammad Noor Amal Azmai Mohammad Sofi’ul Anam Mohammad Sukmanadi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Agil Gumilang Muhammad Ilyas Wahyudi M’ Izi Kumala Lazuardi Sultoni Nabil Fariz Noorrahman Nove Hidajati Novianti, Arindita Niatazya Nusdianto Triakoso Poedji Hastutiek Pramestya, Ninda Rubi Prasinta, Redilla Prastiya, Ragil Angga Pudji Srianto Putri Desi Wulan Sari Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmaniar Larasati Rahmawati, Kadek Rahmi Sugihartuti Rara Irbah Fitriana Karimah Ratna Damayanti Rehman, Saifur Rejeki, Purwo Sri Riza Zainuddin Ahmad Rizki, Andaru Rosyada, Zulfi Nur Amrina Rosyta, Pegy Rozi Sabdoningrum, Emy Koestanti Salleh, Annas Santanumurti, Muhammad Browijoyo Saraswati, Adyayutti Wijang Sari, Putri Desi Wulan Sarmanu, Sarmanu Sheila Marty Yanestria Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soepranianondo, Koesnoto Sony Wibisono Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Sri Pantja Madyawati Suharsono Suharsono Suherni Susilowati Sukmawati Lailatul Jannah Supriyadi Supriyadi Suwarno Suwarno Suwarno Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Titik Yuliarini Trilas Sardjito Tyasningsih, Wiwiek Utomo, Gogik Satrio Margo Wardhana, Dhandy Koesoemo Wardhani, Bantari Wisynu Kusuma Wardhani, Indrayuni Lukitra Wardiana, Nurul Ika Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wibowo, Syahputra Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwiek Tyasningsih Wiwik Misaco Yuniarti Woro Hastuti Setyantini Wurlina, W Yunita, Maya Nurwartanti Yunus , Muchammad Yunus, Muchammad Zulfanisa, Rizky Asrin