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All Journal ACTA VETERINARIA INDONESIANA Media Kedokteran Hewan Briliant: Jurnal Riset dan Konseptual Jurnal Medik Veteriner Jurnal Ilmiah Fillia Cendekia Jurnal Ilmu Peternakan Terapan Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Bantara Journal of Animal Science Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Indonesian Journal of Innovation Studies Journal of Fisheries & Marine Jurnal Agro Veteriner Folia Medica Indonesiana
Al arif, Mohammad Anam
Departemen Peternakan, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C UNAIR Mulyorejo, Surabaya, Jawa Timur, Indonesia 60115
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Oil-Adjuvanted Polyvalent Formalin-killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.82866

Abstract

Graphical Abstract  Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture.   Abstract Motile Aeromonas septicaemia (MAS), predominantly associated with Aeromonas hydrophila, remains a major constraint in giant gourami (Osphronemus goramy) aquaculture. This study evaluated formalin-inactivated A. hydrophila vaccines prepared from MAS-associated field isolates, comparing a monovalent formulation (P2), a non-adjuvanted polyvalent formulation (P3), and an oil-adjuvanted polyvalent formulation (P4) against PBS controls (P1). A total of 240 fish were used (60 per treatment) and assigned to two parallel cohorts (immunology and survival/challenge). Immune endpoints (agglutinating titres, NBT activity, and splenic il-1β and ifn-γ transcription) were assessed on days 7, 14, 21, 35, and 42 post-vaccination. The survival cohort was challenged intraperitoneally at day 21 with a homologous A. hydrophila strain and monitored for 14 days post-challenge. Vaccination was clinically well tolerated and improved survival relative to controls, with P4 showing the highest protection (RPS 81.8%). Agglutinating titres differed by treatment and time; at the peak sampling point (day 35), mean titres in P4 were ~200-fold higher than in P1, and model contrasts indicated significant differences versus controls (p<0.001). Splenic il-1β and ifn-γ transcript levels were higher in vaccinated groups than in controls at later time points. These findings support further evaluation of an oil-adjuvanted polyvalent inactivated A. hydrophila vaccine for gourami, including dose optimisation, extended safety assessment, heterologous challenge, and field validation.
Effect of High Fat Diet on Body Weight, Visceral Fat Weight, and PPARG Expressions on Visceral Fat in Mice Melyana, Cantika Putri; Rejeki, Purwo Sri; Mudjanarko, Sony Wibisono; Herawati, Lilik; Al-Arif, Mohammad Anam
Folia Medica Indonesiana Vol. 57, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Obesity becomes a global epidemic nowadays. The high-fat diet is used as an alternative therapy for obesity. The optimal composition of a high-fat diet to reduce body weight is still unknown. This study aimed to determine which components of a high-fat diet can decrease body weight, visceral fat, and PPARG expression of visceral fat. This study was conducted at the Faculty of Veterinary Medicine, Universitas Airlangga, for three months by using a randomized post-test only control group design. Fifty male mice, 2-3 months old, 18-30 grams were adapted for one week given standard diet AIN93-M, then mice were divided into five groups, namely K1 (control group, 12% fat, 20% protein, 62% carbs); K2 (30% fat, 60% proteins, 0% carbs); K3 (45% fat, 45% protein, 0% carbs); K4 (60% fat, 30% protein, 0% carbs); and K5 (75% fat, 15% protein, 0% carbs). Bodyweight was measured before and after treatment, then the visceral fat and PPARG expressions were evaluated. Statistical comparisons were performed using Statistical Package for the Social Sciences (SPSS) software. After treatment, there were forty-three mice. The body weight and visceral fat weight of the mice with a high-fat diet were decreased. The most significant changes in body weight were in K4 with -9,60 ± 3,806 grams reduction. The bodyweight of mice in K5, slightly increased than K2-K4. This could be caused by the hormesis phenomenon. PPARG expressions decreased in groups with a high-fat diet but increased in K5. The composition of a high-fat diet in group K4 was the most optimal to decrease the body weight, visceral fat, and PPARG expressions in mice
High-Fat Diet Increases Serum HDL, but Not for LDL and HDL/LDL Ratio in MICE Laksana, Iqbal; Rejeki, Purwo Sri; Herawati, Lilik; Arif, Mohammad Anam Al; Wardhani, Indrayuni Lukitra
Folia Medica Indonesiana Vol. 57, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

The use of a ketogenic diet with an increased proportion of fat is rampant lately, both as a therapy or even lifestyle. The use of a ketogenic diet is feared to cause dyslipidemia. It will also lead to atherosclerosis and thrombosis. This study aimed to determine the effect of a high-fat diet on HDL, LDL, and HDL/LDL ratio in mice. Thirty male Mus Musculus, 2-3 mo, 15-25 g divided into five groups diet. K1 (12% fat, 20% protein, 62% carb), K2 (30% fat, 60%protein), K3 (45% fat, 45% protein), K4 (60% fat, 30% protein) and K5 (75% fat, 15% protein) for 4 weeks. Feed and water by adlibitum. Blood for serum was taken from the ventricle, while in the measurement of HDL serum level, the LDL used ELISA. Normality of data analyzed by Shapiro Wilk, and homogeneity by Levene test. HDL serum was analyzed using equality test followed by Games Howell, LDL serum and ratio of HDL/LDL was tested by ANOVA followed by LSD with significance 0,05. HDL serum in K1 (62,50±9.94) mg/dL, K2 (78,40±18,76) mg/dL, K3 (79,00±3,81) mg/dL, K4 (80,00±2,16) mg/dL, and K5 (83,50±5,62) mg/dL with p<0,05 in K1, K2 to K3, K4 and K5. LDL serum in K1 (21,67± 4,80) mg/dL, K2 (23,00±12,70) mg/dL, K3 (18,40±4,34) mg/dL, K4 (24,00 ±1,83) mg/dL and K5 (22,00 ± 4,08) mg/dL with p>0,05. Ratio HDL/LDL K1 (3,01±0,91), K2 (4,10±1,86), K3 (4,53±1,5), K4 (3,35±0,34), and K5 (3,96 ± 1,25) with p>0,05.
Periodic Fasting Treatment on Growth Performance and Feed Efficiency of Nile Tilapia (Oreochromis niloticus, GIFT) Jacob Da Costa Pereira; Salustiano Zaret; Mateus Salvador; Kismiyati; Sapto Adriyono; Ahmad Shofy Mubarak; Mohammad Anam Al Arif
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.81125

Abstract

Fasting is a feed management strategy that reduces feeding frequency without inhibiting fish growth. This strategy involves specific intervals of feed deprivation, allowing fish to exhibit increased appetite once feeding resumes. This study aimed to evaluate the effects of periodic fasting on the growth performance and feed efficiency of GIFT Nile tilapia, as well as to identify the most effective fasting pattern for optimal results. The research used a completely randomized design (CRD) with four treatments, each replicated three times: continuous feeding without fasting (K, control); two days of feeding followed by one day of fasting (2E1F); three days of feeding followed by one day of fasting (3E1F); and four days of feeding followed by one day of fasting (4E1F). The primary parameters observed were absolute weight gain (g), absolute length growth (cm), and feed efficiency. Data were analyzed using the Kruskal–Wallis test (p < 0.05), followed by post hoc comparisons using independent samples tests to determine significant differences among treatments. The results showed that periodic fasting had a significant effect (p < 0.05) on absolute weight gain, absolute length growth, and feed efficiency of GIFT Nile tilapia. The best performance was observed in treatment 4E1F (four days of feeding followed by one day of fasting), while the lowest performance occurred in treatment 2E1F (two days of feeding followed by one day of fasting).
Feasibility and Sensitivity Analysis of Broiler Chicken Farming with the Partnership Pattern of the “Lumajang Broiler Group” in Lumajang Regency Fara Abafuri Permata; Imam Mustofa; Sunaryo Hadi Warsito; Mohammad Anam Al Arif; Sri Hidanah; Erma Safitri; Widya Paramita Lokapirnasari; Emy Koestanti Sabdoningrum
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.83597

Abstract

This study aimed to evaluate the financial feasibility and sensitivity of the broiler chicken farming business of the “Paguyuban Broiler Lumajang” Livestock Group in Lumajang Regency. The research employed a descriptive observational method using a survey approach, involving 20 farmers who were categorized into two groups based on partnership companies (national and multinational companies). Feasibility analysis was conducted using four key financial indicators: Net Present Value (NPV), Average Rate of Return (ARR), Benefit–Cost Ratio (B/C Ratio), and Payback Period (PP). Sensitivity analysis focused on the impact of changes in feed prices (increases of 5%, 10%, and 15%) and broiler selling prices (decreases of 5%, 10%, and 15%). The results indicated that all groups were financially feasible, with positive NPV values, ARR significantly exceeding prevailing interest rates, PP under five years, and B/C Ratio values above 1. Sensitivity analysis revealed that a decrease in broiler selling prices had a greater impact on profit generation than an increase in feed prices. In conclusion, all groups in the “Paguyuban Broiler Lumajang” were financially viable and showed strong potential for further development. Government support in stabilizing broiler prices and feed costs is essential to ensure business sustainability and farmers’ welfare.
Co-Authors A. Shofy Mubarak Agung Prasetyo Agustono, Bodhi Aksono HP., Eduardus Bimo Alfanindya, Evita Aisyah Ali Agus Amal, Mohammad Noor Azmai Anastasya, Jelita Andreas Berny Yulianto, Andreas Berny Andriani, Asafarid Annas Salleh Annisa Nur Aulia Purwantoro Ardianto Ardianto Aris Puji Nugroho Aryaloka, Suhita Ayuti, Siti Rani Balgis Al Basyarahil Bambang Prajogo Eko Wardojo Cantika Putri Melyana Caska - Chandra, Evania Haris Chusniati, Sri Dadik Rahardjo Dadik Rahardjo, Dadik Daruti Dinda Nindarwi Devi Sri Rohmani Dian Ayu Permatasari Dimas Kunto Satrio Dita Wisudyawati Djoko Legowo Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Emy Koestanti Sabdoningrum Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri Erma Safitri ERMA SAFITRI Faiqoh, Berliana Elok Faisal Fikri Fara Abafuri Permata Fatin Fadhilah Hasib Fauzia, Kartika Afrida Fauziah, Ima Fuddin, Muhammad Nazar Gandul Atik Yuliani Gazali Salim Gesa Cantya Primatara Hadiah Fitriyah Hani Plumeriastuti Hapsari, Tiara Hasib, Abdullah Hendarti, Gracia Angelina HERAWATI, LILIK Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Hussain, Muhammad Asif Imam Mustofa IMAM MUSTOFA Iqbal Laksana Ira Sari Yudaniayanti Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Jacob Da Costa Pereira Joel Jeevan Raj S/O Yogarajah Kadek Rachmawati Khairullah, Aswin Rafif Khanand, Nadeem Kirana, Amelia Lintang Putri Kismiyati , Koesnoto Soepranianondo Koesnoto Supranianondo Kuncorojakti, Suryo Kuncorojati, Suryo Kurniasih, Dea Anita Ariani Kusala, Muhammad Khaliim Jati Laksana, Iqbal Lilik Maslachah Lovela, Aprinda Ratna Ma'ruf, Muchammad Achsinul Fikri Mafruchati, Maslichah Maghfiroh, Nurutin Tutur Bifatikhatil M Mateus Salvador Melyana, Cantika Putri Miarsono, Sigit Mirni Lamid Mirni Lamid Mochamad Lazuardi Moh. Sofiul Anam Moh. Zaky ‘Ubaidillah Mohammad Noor Amal Azmai Mohammad Sofi’ul Anam Mohammad Sukmanadi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Agil Gumilang Muhammad Ilyas Wahyudi M’ Izi Kumala Lazuardi Sultoni Nabil Fariz Noorrahman Nenny Harijani Nina Nurmalia Dewi Nove Hidajati Novianti, Arindita Niatazya Nusdianto Triakoso Poedji Hastutiek Pramestya, Ninda Rubi Prasinta, Redilla Prastiya, Ragil Angga Pudji Srianto Putri Desi Wulan Sari Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmaniar Larasati Rahmawati, Kadek Rahmi Sugihartuti Rara Irbah Fitriana Karimah Ratna Damayanti Rehman, Saifur Rejeki, Purwo Sri Riza Zainuddin Ahmad Rizki, Andaru Rosyada, Zulfi Nur Amrina Rosyta, Pegy Rozi Salleh, Annas Salustiano Zaret Santanumurti, Muhammad Browijoyo Sapto Adriyono Saputro, Amung Logam Saraswati, Adyayutti Wijang Sari, Putri Desi Wulan Sarmanu, Sarmanu Setyantini, Woro Hastuti Sheila Marty Yanestria Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soepranianondo, Koesnoto Sony Wibisono Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Sri Pantja Madyawati Suharsono Suharsono Suherni Susilowati Sukmawati Lailatul Jannah Supriyadi Supriyadi Suwarno Suwarno Suwarno Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Titik Yuliarini Trilas Sardjito Tyasningsih, Wiwiek Utomo, Gogik Satrio Margo Wardhana, Dhandy Koesoemo Wardhani, Bantari Wisynu Kusuma Wardhani, Indrayuni Lukitra Wardiana, Nurul Ika Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wibowo, Syahputra Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwiek Tyasningsih Wiwik Misaco Yuniarti Wurlina, W Yunita, Maya Nurwartanti Yunus , Muchammad Yunus, Muchammad Zulfanisa, Rizky Asrin