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All Journal ACTA VETERINARIA INDONESIANA Media Kedokteran Hewan Briliant: Jurnal Riset dan Konseptual Caraka Tani: Journal of Sustainable Agriculture Jurnal Medik Veteriner Jurnal Ilmiah Fillia Cendekia Jurnal Ilmu Peternakan Terapan Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Bantara Journal of Animal Science Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat LOSARI: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Innovation Studies Journal of Fisheries & Marine Jurnal Agro Veteriner Folia Medica Indonesiana
Al arif, Mohammad Anam
Departemen Peternakan, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C UNAIR Mulyorejo, Surabaya, Jawa Timur, Indonesia 60115
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Supplementation with mineral mix or concentrate booster restored fertility in cows following foot-and-mouth disease infection Aris Puji Nugroho; Ali Agus; Moh. Sofiul Anam; Suherni Susilowati; Herry Agoes Hermadi; Dian Ayu Permatasari; Mohammad Anam Al Arif
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.127-137

Abstract

This study aimed to evaluate the effects of supplementing mineral mix and concentrate booster on the fertility of cows recovering from foot-and-mouth disease (FMD). Eighteen post-FMD cows were assigned to three treatment groups. Cows in the T0 group received a standard feed consisting of elephant grass and a local concentrate. In the T1 group, cows were provided the standard feed supplemented with 40 g/day of mineral mix, while cows in the T2 group received the standard feed supplemented with 600 g/day of concentrate booster. The feeding treatments were applied for 42 days, and fresh drinking water was provided ad libitum. The results showed that the onset of estrus occurred significantly earlier (p <0.05) compared to the anestrus period experienced during FMD infection. Both supplemented groups exhibited higher (p <0.05) estrus rates, pregnancy rates, and calving rates than the T0 group. The interval between the last estrus and the initiation of additional feeding was considerably longer than the onset of estrus observed after treatment. The pH and ferning scores of cervical mucus in cows from the T0 group were lower than those in the T1 and T2 groups. However, no significant differences (p >0.05) were found in cervical mucus pH or ferning scores between the T1 and T2 groups, between pregnant and non-pregnant cows, or between offspring sex categories. In conclusion, supplementation with either a mineral mix or a concentrate booster improved fertility parameter, including estrus rate, pregnancy rate, and calving rate, in cows recovering from FMD.
Oil-Adjuvanted Polyvalent Formalin-killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.82866

Abstract

Graphical Abstract  Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture.   Abstract Motile Aeromonas septicaemia (MAS), predominantly associated with Aeromonas hydrophila, remains a major constraint in giant gourami (Osphronemus goramy) aquaculture. This study evaluated formalin-inactivated A. hydrophila vaccines prepared from MAS-associated field isolates, comparing a monovalent formulation (P2), a non-adjuvanted polyvalent formulation (P3), and an oil-adjuvanted polyvalent formulation (P4) against PBS controls (P1). A total of 240 fish were used (60 per treatment) and assigned to two parallel cohorts (immunology and survival/challenge). Immune endpoints (agglutinating titres, NBT activity, and splenic il-1β and ifn-γ transcription) were assessed on days 7, 14, 21, 35, and 42 post-vaccination. The survival cohort was challenged intraperitoneally at day 21 with a homologous A. hydrophila strain and monitored for 14 days post-challenge. Vaccination was clinically well tolerated and improved survival relative to controls, with P4 showing the highest protection (RPS 81.8%). Agglutinating titres differed by treatment and time; at the peak sampling point (day 35), mean titres in P4 were ~200-fold higher than in P1, and model contrasts indicated significant differences versus controls (p<0.001). Splenic il-1β and ifn-γ transcript levels were higher in vaccinated groups than in controls at later time points. These findings support further evaluation of an oil-adjuvanted polyvalent inactivated A. hydrophila vaccine for gourami, including dose optimisation, extended safety assessment, heterologous challenge, and field validation.
Effect of High Fat Diet on Body Weight, Visceral Fat Weight, and PPARG Expressions on Visceral Fat in Mice Melyana, Cantika Putri; Rejeki, Purwo Sri; Mudjanarko, Sony Wibisono; Herawati, Lilik; Al-Arif, Mohammad Anam
Folia Medica Indonesiana Vol. 57, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Obesity becomes a global epidemic nowadays. The high-fat diet is used as an alternative therapy for obesity. The optimal composition of a high-fat diet to reduce body weight is still unknown. This study aimed to determine which components of a high-fat diet can decrease body weight, visceral fat, and PPARG expression of visceral fat. This study was conducted at the Faculty of Veterinary Medicine, Universitas Airlangga, for three months by using a randomized post-test only control group design. Fifty male mice, 2-3 months old, 18-30 grams were adapted for one week given standard diet AIN93-M, then mice were divided into five groups, namely K1 (control group, 12% fat, 20% protein, 62% carbs); K2 (30% fat, 60% proteins, 0% carbs); K3 (45% fat, 45% protein, 0% carbs); K4 (60% fat, 30% protein, 0% carbs); and K5 (75% fat, 15% protein, 0% carbs). Bodyweight was measured before and after treatment, then the visceral fat and PPARG expressions were evaluated. Statistical comparisons were performed using Statistical Package for the Social Sciences (SPSS) software. After treatment, there were forty-three mice. The body weight and visceral fat weight of the mice with a high-fat diet were decreased. The most significant changes in body weight were in K4 with -9,60 ± 3,806 grams reduction. The bodyweight of mice in K5, slightly increased than K2-K4. This could be caused by the hormesis phenomenon. PPARG expressions decreased in groups with a high-fat diet but increased in K5. The composition of a high-fat diet in group K4 was the most optimal to decrease the body weight, visceral fat, and PPARG expressions in mice
High-Fat Diet Increases Serum HDL, but Not for LDL and HDL/LDL Ratio in MICE Laksana, Iqbal; Rejeki, Purwo Sri; Herawati, Lilik; Arif, Mohammad Anam Al; Wardhani, Indrayuni Lukitra
Folia Medica Indonesiana Vol. 57, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

The use of a ketogenic diet with an increased proportion of fat is rampant lately, both as a therapy or even lifestyle. The use of a ketogenic diet is feared to cause dyslipidemia. It will also lead to atherosclerosis and thrombosis. This study aimed to determine the effect of a high-fat diet on HDL, LDL, and HDL/LDL ratio in mice. Thirty male Mus Musculus, 2-3 mo, 15-25 g divided into five groups diet. K1 (12% fat, 20% protein, 62% carb), K2 (30% fat, 60%protein), K3 (45% fat, 45% protein), K4 (60% fat, 30% protein) and K5 (75% fat, 15% protein) for 4 weeks. Feed and water by adlibitum. Blood for serum was taken from the ventricle, while in the measurement of HDL serum level, the LDL used ELISA. Normality of data analyzed by Shapiro Wilk, and homogeneity by Levene test. HDL serum was analyzed using equality test followed by Games Howell, LDL serum and ratio of HDL/LDL was tested by ANOVA followed by LSD with significance 0,05. HDL serum in K1 (62,50±9.94) mg/dL, K2 (78,40±18,76) mg/dL, K3 (79,00±3,81) mg/dL, K4 (80,00±2,16) mg/dL, and K5 (83,50±5,62) mg/dL with p<0,05 in K1, K2 to K3, K4 and K5. LDL serum in K1 (21,67± 4,80) mg/dL, K2 (23,00±12,70) mg/dL, K3 (18,40±4,34) mg/dL, K4 (24,00 ±1,83) mg/dL and K5 (22,00 ± 4,08) mg/dL with p>0,05. Ratio HDL/LDL K1 (3,01±0,91), K2 (4,10±1,86), K3 (4,53±1,5), K4 (3,35±0,34), and K5 (3,96 ± 1,25) with p>0,05.
Co-Authors Agung Prasetyo Agustono, Bodhi Aksono HP., Eduardus Bimo Alfanindya, Evita Aisyah Ali Agus Amal, Mohammad Noor Azmai Amung Logam Saputro Anastasya, Jelita Andreas Berny Yulianto, Andreas Berny Andriani, Asafarid Annas Salleh Annisa Nur Aulia Purwantoro Ardianto Ardianto Aris Puji Nugroho Aryaloka, Suhita Ayuti, Siti Rani Balgis Al Basyarahil Bambang Prajogo E.W Cantika Putri Melyana Caska - Chandra, Evania Haris Chusniati, Sri Dadik Rahardjo Dadik Rahardjo, Dadik Daruti Dinda Nindarwi Devi Sri Rohmani Dewi, Nina Nurmalia Dian Ayu Permatasari Dimas Kunto Satrio Dita Wisudyawati Djoko Legowo Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri ERMA SAFITRI Faiqoh, Berliana Elok Faisal Fikri Fatin Fadhilah Hasib Fauzia, Kartika Afrida Fauziah, Ima Fuddin, Muhammad Nazar Gandul Atik Yuliani Gazali Salim Gesa Cantya Primatara Hadiah Fitriyah Hani Plumeriastuti Hapsari, Tiara Harijani, Nenny Hasib, Abdullah Hendarti, Gracia Angelina HERAWATI, LILIK Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Hussain, Muhammad Asif IMAM MUSTOFA Iqbal Laksana Ira Sari Yudaniayanti Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Joel Jeevan Raj S/O Yogarajah Kadek Rachmawati Khairullah, Aswin Rafif Khanand, Nadeem Kirana, Amelia Lintang Putri Koesnoto Soepranianondo Koesnoto Supranianondo Kuncorojakti, Suryo Kuncorojati, Suryo Kurniasih, Dea Anita Ariani Kusala, Muhammad Khaliim Jati Laksana, Iqbal Lilik Maslachah Lovela, Aprinda Ratna Ma'ruf, Muchammad Achsinul Fikri Mafruchati, Maslichah Maghfiroh, Nurutin Tutur Bifatikhatil M Melyana, Cantika Putri Miarsono, Sigit Mirni Lamid Mirni Lamid Mochamad Lazuardi Moh. Sofiul Anam Moh. Zaky ‘Ubaidillah Mohammad Noor Amal Azmai Mohammad Sofi’ul Anam Mohammad Sukmanadi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Agil Gumilang Muhammad Ilyas Wahyudi M’ Izi Kumala Lazuardi Sultoni Nabil Fariz Noorrahman Nove Hidajati Novianti, Arindita Niatazya Nusdianto Triakoso Poedji Hastutiek Pramestya, Ninda Rubi Prasinta, Redilla Prastiya, Ragil Angga Pudji Srianto Putri Desi Wulan Sari Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmaniar Larasati Rahmawati, Kadek Rahmi Sugihartuti Rara Irbah Fitriana Karimah Ratna Damayanti Rehman, Saifur Rejeki, Purwo Sri Riza Zainuddin Ahmad Rizki, Andaru Rosyada, Zulfi Nur Amrina Rosyta, Pegy Rozi Sabdoningrum, Emy Koestanti Salleh, Annas Santanumurti, Muhammad Browijoyo Saraswati, Adyayutti Wijang Sari, Putri Desi Wulan Sarmanu, Sarmanu Sheila Marty Yanestria Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soepranianondo, Koesnoto Sony Wibisono Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Sri Pantja Madyawati Suharsono Suharsono Suherni Susilowati Sukmawati Lailatul Jannah Supriyadi Supriyadi Suwarno Suwarno Suwarno Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Titik Yuliarini Trilas Sardjito Tyasningsih, Wiwiek Utomo, Gogik Satrio Margo Wardhana, Dhandy Koesoemo Wardhani, Bantari Wisynu Kusuma Wardhani, Indrayuni Lukitra Wardiana, Nurul Ika Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wibowo, Syahputra Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwiek Tyasningsih Wiwik Misaco Yuniarti Woro Hastuti Setyantini Wurlina, W Yunita, Maya Nurwartanti Yunus , Muchammad Yunus, Muchammad Zulfanisa, Rizky Asrin