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All Journal e-GIGI Prosiding Seminar Biologi Indonesian Journal of Cancer Chemoprevention Journal of Mathematical and Fundamental Sciences Indonesian Journal of Cancer Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Padjadjaran Journal of Dentistry Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Kreativitas PKM SONDE (Sound of Dentistry) Jurnal Muara Sains, Teknologi, Kedokteran dan Ilmu Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Poltekita: Jurnal Pengabdian Masyarakat ABDI MOESTOPO: Jurnal Pengabdian pada Masyarakat Narra J Proceeding Biology Education Conference Indonesian Journal of Cancer Jurnal Kedokteran Gigi Terpadu JURNAL ABDI MASYARAKAT INDONESIA (JAMIN) Jurnal Abdimas Kesehatan Terpadu
Ferry Sandra
Department Of Biochemistry And Molecular Biology, Division Of Oral Biology, Faculty Of Dentistry, Universitas Trisakti
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Other

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Human Umbilical Cord Blood Serum Has Higher Potential in Inducing Proliferation of Fibroblast than Fetal Bovine Serum Sandra, Ferry; Lahirin, Rita
Molecular and Cellular Biomedical Sciences Vol 1, No 2 (2017)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v1i2.4

Abstract

Background: Cytokines and growth factors were reported to play an important role in stimulating fibroblast proliferation. In vitro culture, fibroblast is mostly culture in medium containing fetal bovine serum (FBS).  Human umbilical cord blood (hUCB) has been reported to have low immunogenic property and potential in wound healing, so therefore hUCB serum (hUCBS) could be potential and were investigated in current study.Materials and Methods: Five hUCBs were collected from healthy volunteers with normal delivering procedure. hUCB was ex utero immediately collected from umbilical vein in vacutainers and processed. NIH3T3 cells were cultured in DMEM with 10% FBS or 5-20% hUCBS for 48 hours. Cells were then quantified using MTT assay. Protein concentration of FBS and hUCBS were quantified using Bradford assay.Results: NIH3T3 cells density grown in DMEM with 10% FBS was the lowest. NIH3T3 cells densities were increased along with the increment of hUCBS concentrations. MTT results showed that average number of NIH3T3 cells grown in DMEM with 10% FBS was 6,185±1,243. Meanwhile average numbers of NIH3T3 cells grown in DMEM with 5%, 10% and 20% hUCBS were 8,126±628, 9,685±313 and 12,200±304, respectively. Average numbers of NIH3T3 cells grown in DMEM with 5% hUCBS were significantly higher than the ones with 10% FBS (p=0.000). Bradford results showed that concentration of hUCBS was significantly higher than the one of FBS (p=0.000).Conclusion: hUCBS could induce higher proliferation rate of NIH3T3 cells than FBS. Hence hUCBS could be suggested as an alternate of FBS in inducing fibroblast.Keywords: NIH3T3, fibroblast, UCB, serum, FBS, proliferation
Caffeic Acid Induced Apoptosis in MG63 Osteosarcoma Cells Through Activation of Caspases Sandra, Ferry; Sidharta, Meta Ariyani
Molecular and Cellular Biomedical Sciences Vol 1, No 1 (2017)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v1i1.6

Abstract

Background: Caffeic acid has been reported that when it is combined with all-trans retinoic acid, it can inhibit proliferation activity of SaOS-2 or OSA-01 cells. In addition, caffeic acid merely could reduce cell viability of SaOS-2 cells. However, there is not any study in caffeic acids possible effect to induce apoptosis in osteosarcoma cell.Materials and Methods: MG-63 cells were cultured in Dulbecco’s Modified Eagle Medium containing 10% fetal bovine serum. Cells were treated with various concentrations of caffeic acid. Apoptosis were analyzed with Sub-G1 assay and activation of caspase-8, -9, and -3 were analyzed with immunoblotting. Caffeic acid-induced percentage of apoptotic cells and cleaved-8, -9, -3 were then statistically analyzed.Results: Sub-G1 results showed that caffeic acid significantly induced apoptosis in MG-63 osteosarcoma cells in concentration dependent manner. Immunoblotting results showed that caffeic acid induced cleavage of caspase-8, -9 and -3. Cleaved-caspase-8 and -9 were increased at 1-hour treatment of caffeic acid, while cleaved-caspase 3 was increased markedly at 6-hours treatment of caffeic acid.Conclusions: Caffeic acid induces apoptosis significantly in concentration dependent manner through caspase-dependent intrinsic apoptotic pathway.Keywords: caffeic acid, osteosarcoma, MG-63, apoptosis, caspase
Relationship between sRAGE and hsCRP as Markers of Cardiovascular Disease Risk Factors in Diabetic and Non-Diabetic Men with Central Obesity Hamuaty, Rambu Beppy; Sukmawati, Indriyanti Rafi; Sandra, Ferry
Molecular and Cellular Biomedical Sciences Vol 1, No 2 (2017)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v1i2.14

Abstract

Background: Interaction between advanced glycation end product (AGE) and receptor for AGE (RAGE) triggers the escalation of inflammatory cytokine expressions. High-sensitivity C-reactive protein (hsCRP), an important inflammatory marker, has been reported to be modulated by soluble RAGE (sRAGE). However, the relationship between hsCRP and sRAGE in diabetes was not clearly described. Therefore present study was conducted to determine the relationship between sRAGE with hsCRP in men with central obese diabetic and non-diabetic. Materials and Methods: Adult men aged 25-60 years with central obese diabetes and non-diabetes, were recruited. Patient’s profiles were collected before the physical and blood examination. Physical examinations were conducted by measuring waist/abdomen, blood pressure, height, and weight. The routine blood test was performed to obtain concentrations of fasting blood glucose, HbA1c, hsCRP and sRAGE level.Results: Fifty-seven subjects with central obese and waist size ≥90 cm were selected. It was found that hsCRP values were significantly different (p=0.000) in HbA1c <6.5% dan HbA1c ≥6.5% groups. There was an inverse relationship between hsCRP and sRAGE levels for both in HbA1c <6.5% (r=-0.073) and HbA1c≥6.5% (r=-0.022) groups. In HbA1c ≥6.5% group, sRAGE showed strong positive correlation with 1 mg/dL ≤ hsCRP <3 mg/dL group (r>0.5).Conclusions: In the early stages of diabetes with hsCRP <1 mg/dL, the protective function was demonstrated with greater sRAGE levels. However, in further phase with 3 ≤ hsCRP < 10 mg/dL, the level of sRAGE was low, which is assumed to be associated with complications. Hence, sRAGE could be suggested as a complementary marker for hsCRP to evaluate diabetic men with central obesity.Keywords: sRAGE, hsCRP, diabetes, HbA1c, central obesity
CONDITIONED MEDIUM DARI KULTUR PRIMER SEL SYARAF MUS MUSCULUS Puspitasari, Riris L.; Boediono, Arief; Sandra, Ferry
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (679.322 KB)

Abstract

Secara  in vitro,  Embryonic Stem Cell  (ESC) dapat diarahkan perkembangannya menjadi sel neuron dan sel glia. Conditionedmedium  dari kultur primer sel syaraf mengandung sejumlah faktor pertumbuhan antara lain  nerve growth factor (NGF), glial derived-neurotrophic factor (GDNF), nestin, dan glial fibrillary acidic protein (GFAP). Dengan melakukan purifikasi protein yang terkandung  di dalam CM, maka diharapkan spektrum protein yang ada menjadi lebih sempit sehingga protein target dapat terdeteksi. Penelitian ini mempelajari kultur primer sel syaraf yang berasal dari hemisfer Mus musculus. Tujuan penelitian adalah untuk mendapatkan CM dari kultur primer sel syaraf  Mus musculus. Medium yang digunakan adalah Dulbecco?s Modified Eagle?s Medium (DMEM) highglucose FBS 10%. Penggantian medium kultur dilakukan setiap 2 hari sekali. Kepadatan sel sekitar 32x103 sel/2 cm2. Setelah hari ke-4 terlihat adanya pertumbuhan neuron bipolar dan neural progenitor cell (NPC). Sel-sel astrosit akan teramati ketika periode kultur diperpanjang. Sel mengalami konfluensi setelah 12 hari kultur. Sel-sel yang tumbuh berguna untuk penjelasan neurogenesis. Kultur primer sel syaraf secara monolayer yang berasal dari hemisfer neonatus mampu mendukung  pertumbuhan sel yang tergolong sebagai neurogenic dan nonneurogenic.  Kata kunci: kultur primer, sel syaraf, conditioned medium, neural progenitor cell, neurogenesis.
Knowledge and Attitudes Regarding Covid-19 Prevention in the Music Community Andayani, Lia H.; Sandra, Ferry; Soulissa, Abdul G.; Handojo, James
e-GiGi Vol 10, No 1 (2022): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v10i1.35659

Abstract

Abstract: The escalation of COVID-19 cases both in morbidity and mortality rates in Daerah Khusus Ibukota (DKI) Jakarta and its surrounding areas (JABODETABEK) continues to occur. Adherence in implementing health protocols depends on the knowledge and attitudes of the individuals. This study aimed to assess the knowledge and attitude regarding Covid-19 prevention in an informal community, namely music community. This study was conducted online with 44 participants obtained through convenience sampling method. Participants filled out the questionnaires covering individual characteristics, general knowledge regarding Covid-19, and attitudes towards protective measures against Covid-19. The Mann-Whitney test was used to analyze the differences in the mean score of knowledge based on selected variables. There were 52.4% of participants that had low level of knowledge. Meanwhile, 86.4% of participants had positive attitude towards protective measures against Covid-19. Significant differences were found in the mean scores of knowledge based on age (p=0.001), place of residence (p=0.004), and occupational status (p=0.032). In conclusion, most participants of the music community in Jakarta and surrounding areas have low level of knowledge, however, they have positive attitude towards protective measures against Covid-19.Keywords: Covid-19; knowledge; attitude; music community Abstrak: Peningkatan kasus Covid-19 baik dalam angka morbiditas maupun mortalitas di wilayah Daerah Khusus Ibukota (DKI) Jakarta dan sekitarnya (JABODETABEK) masih berlangsung. Kepatuhan dalam menerapkan protokol kesehatan bergantung pada pengetahuan dan sikap individu. Penelitian ini bertujuan untuk mengetahui gambaran pengetahuan dan sikap mengenai pencegahan Covid-19 pada salah satu komunitas informal di masyarakat, yaitu komunitas musik. Penelitian ini dilakukan secara daring dengan 44 peserta yang didapatkan melalui metode convenience sampling. Peserta mengisi kuesioner survei yang berisi data karateristik individu, pengetahuan umum, dan sikap terkait Covid-19. Uji Mann-Whitney digunakan untuk melihat perbedaan rerata nilai pengetahuan berdasarkan setiap variabel. Hasil penelitian mendapatkan sebanyak 52,4% responden memiliki tingkat pengetahuan rendah namun 86,4% responden memiliki sikap positif terhadap upaya pencegahan penularan Covid-19. Perbedaan rerata nilai pengetahuan secara bermakna ditemukan berdasarkan usia (p=0,001), wilayah tinggal (p=0,004), dan status pekerjaan (p=0,032). Simpulan penelitian ini ialah sebagian besar komunitas musik di DKI Jakarta dan sekitarnya memiliki tingkat pengetahuan rendah namun memiliki sikap positif terhadap upaya pencegahan penularan Covid -19. Kata kunci: Covid-19; pengetahuan; sikap; komunitas musik
Bactericidal and cytotoxic effects of Erythrina fusca leaves aquadest extract Janti Sudiono; Ferry Sandra; Nadya Saputri Halim; Timotius Andi Kadrianto; Melinia Melinia
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 1 (2013): March 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.705 KB) | DOI: 10.20473/j.djmkg.v46.i1.p9-13

Abstract

Background: Empirically, Erythrina fusca has been used as traditional herb for its antibacterial and antiinflammation properties. Periodontal disease is one of the most oral infectious diseases with microorganism predominated as the contributing factors. Porphyromonas gingivalis (P. gingivalis) is one of the main bacteria pathogen found in periodontal diseases. Purpose: The purpose of this study was to examine the bactericidal effect of Erythrina fusca Leaves Aquadest Extract (EFLAE) at various concentrations on P. gingivalis and cytotoxic effect on fibroblast. Methods: Pure P. gingivalis was cultured in Brain Heart Infusion (BHI) medium for 24 hours with or without various concentrations of treatment of EFLAE. Calculation and statistical analysis of remaining bacteria were performed by inhibitory zone method to evaluate the EFLAE bactericidal effect and compared to chlorhexidine as positive control. To evaluate the cytotoxic effect, NIH 3T3 cells were cultured in Dulbecco’s Modification of Eagle’s Medium (DMEM) containing of 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, pH 7.2, in 5% CO2, and stored in humidified incubator under temperature 370 C. Cells were treated with/without various concentrations of EFLAE for 48 hours. The viable cells were then counted using 3-(4,5- Dimethylthiazol-2-yl)-2,5 diphenyl tetrazodium bromide (MTT) method. Results: EFLAE have bactericidal effect on P. gingivalis in a concentration dependent manner starting from 78%. The concentration of 90% EFLAE had stronger bactericidal effect (35.004 ± 1.546) than those of chlorhexidine as positive control (32.313 ± 1.619). One-way ANOVA showed significant bactericidal effect differences among concentrations of EFLAE and chlorhexidine (p<0.05) while Tuckey HSD test showed significant difference only between lower concentration of EFLAE (78%, 79%) and chlorhexidine. With the highest concentration of EFLAE (100%) applied in the bactericidal test, no cytotoxic effect of EFLAE on NIH 3T3 cells was detected. Conclusion: EFLAE could inhibit the growth of P. gingivalis in a concentration dependent manner, starting from 78%. There was no evidence of EFLAE’s cytotoxic effect on fibroblast.Latar belakang: Erythrina fusca telah digunakan secara empiris sebagai tanaman obat tradisional untuk khasiat antibakteri dan antiradang. Penyakit periodontal merupakan salah satu penyakit infeksi mulut terbanyak dengan mikroorganisme sebagai faktor kontributor utama. Porphyromonas gingivalis (P. gingivalis) merupakan salah satu bakteri patogen utama yang ditemukan pada penyakit periodontal. Tujuan: Tujuan penelitian ini untuk mengamati efek bakterisid terhadap P. gingivalis dan efek sitotoksik terhadap sel fibroblast dari beberapa konsentrasi ekstrak akuades daun Erythrina fusca (EFLAE). Metode: P. gingivalis murni dikultur pada medium Brain Heart Infusion (BHI) selama 24 jam dengan atau tanpa pemberian beberapa konsentrasi EFLAE. Perhitungan dan analisis statistik terhadap bakteri yang masih hidup dilakukan dengan metode zona hambat untuk mengevaluasi efek bakterisid EFLAE dibandingkan dengan chlorhexidine sebagai kontrol positif. Untuk mengevaluasi efek sitotoksik, digunakan kultur sel NIH 3T3 pada medium Dulbecco’s Modification of Eagle’s Medium (DMEM) yang berisi fetal bovine serum (FBS) 10% dan penicillin-streptomycin 1%, pH 7.2, dalam CO2 5%, dan diinkubasi pada suhu 37° C. Sel diberi perlakuan dengan atau tanpa beberapa konsentrasi EFLAE selama 48 jam, kemudian sel yang masih hidup dihitung menggunakan metode 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazodium bromide (MTT). Hasil: EFLAE mempunyai efek bakterisid terhadap P. gingivalis mengikuti kenaikan konsentrasinya dimulai dari 78%. Pada konsentrasi 90%, EFLAE menunjukkan efek bakterisid lebih kuat (35.004 ± 1.546) dibandingkan dengan chlorhexidine (32.313 ± 1.619) sebagai kontrol positif ANOVA-1 jalan menunjukkan perbedaan efek bakterisid yang bermakna di antara beberapa konsentrasi EFLAE dan chlorhexidine (p<0.05) sedangkan uji Tuckey HSD menunjukkan perbedaan bermakna hanya ditemukan antara konsentrasi EFLAE yang lebih rendah (78%, 79%) dengan chlorhexidine. Efek sitotoksik terhadap sel NIH 3T3 tidak terdeteksi pada pemberian konsentrasi tertinggi EFLAE (100%) yang telah diaplikasikan pada uji bakterisid. Kesimpulan: EFLAE dapat menghambat pertumbuhan P. gingivalis sesuai dengan konsentrasinya dimulai dari 78%. Tidak ada efek sitotoksik EFLAE terhadap sel fibroblast.
Expression of CD133 in various premalignant and proliferative lesions Rahmi Amtha; Indrayadi Gunardi; Ferry Sandra; Diah Savitri Ernawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 2 (2015): June 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.723 KB) | DOI: 10.20473/j.djmkg.v48.i2.p64-68

Abstract

Background: In Jakarta, oral squamous cell carcinoma (OSCC) usually detected in late stage with very low survival rate ofabout 1.1 years. OSCC may be preceded by premalignant lesion, so that early detection of the lesion may decrease the mortality rate due to oral malignancy. CD133 is a hematopoietic stem cell that play role in tissue regeneration, inflammation and tumor. Upregulated of CD133 was reported on tumor progression. Purpose: The aim of study is to determine circulating CD133 expression on premalignant (PML) and proliferative (PL) lesion. Method: Observational research was carried out on patients who seek treatment of PML and PL at Oral Medicine clinic. CD133 was taken from peripheral blood serum, examined using PCR. Data was analyzed by Chi square test. Result: 15 subjects (each of five subjects for PML, PL and control) consist of 40% male and 60% female. Age group of above 41 years old was most affected PML and PL (66.7%). Tongue is common site for oral lesion (40%). There is a significant different of circulating CD133 rate among all groups lesion (p=0.039). Conclusion: CD133 express differently in premalignant and proliferative lesions.
Ser81 Survivin Induced Protein Kinase A (PKA)-dependent Phosphatidylinositol 3-kinase (PI3K) Activity Ferry Sandra; Roya Khosravi-Far
The Indonesian Biomedical Journal Vol 6, No 3 (2014)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v6i3.28

Abstract

BACKGROUND: Our previous report showed that phosphorylated-survivin at Ser81 induces survivin back loop to activate protein kinase A (PKA) in the cytoprotection mechanism. Activated PKA could possibly induce the cytoprotection via Phosphatydilinositol 3-kinase (PI3K). Therefore our current study was conducted to investigate the possibility of survivin-PKA-PI3K signaling pathway.METHODS: Viral productions by BOSC23 cells of Survivin, Antisense Survivin (Survivin-AS) and Ser81Ala mutant (Survivin-S81A) in pMSCV-IRES-GFP vector with cytomegalovirus (CMV) promoter were conducted. L929 cells were pretreated with/without PKI 6-22 amide and infected with viral particle of Survivin, Survivin-AS, Survivin-S81A or vector only. Cells were harvested, lysed and immunoprecipitated with anti-PI3K (p85) antibody and immunoblotted to detect PI3K (p85) and phospho-(Tyr) p85 PI3K. To confirm PI3K activation, PI3K Activity Assay was conducted by using phosphoinositide fraction containing PtdIns(4,5)P2 and [32P]ATP.RESULTS: Immunoblot and PI3K activity results showed similar results. Upon infection of virus with survivin, a markedly increased level of tyrosine phosphorylation of p85 PI3K or PI3K activity in L929 cells was seen. Low levels of tyrosine phosphorylation of p85 PI3K or PI3K activity were observed for Survivin-AS and Survivin-S81A-viral-infected L929 cells. With higher concentrations of Survivin-viral-infection, levels of tyrosine phosphorylation of p85 PI3K or PI3K activity in L929 cells were gradually increased. However, when L929 cells were pretreated with PKI 6-22 amide, prior to Survivin-viral-infection, level of tyrosine phosphorylation level of p85 PI3K or PI3K activity was detected much lower.CONCLUSION: Our result suggest that Ser81 Survivin play role in inducing PI3K activation and the Survivin-PI3K signaling pathway was PKA-dependent.KEYWORDS: Ser81, Survivin, PKA, PI3K, L929
Combination of Fibrinogen and High-sensitivity C-reactive Protein Measurements is Potential in Identification of Acute Coronary Syndrome Djanggan Sargowo; Ferry Sandra
The Indonesian Biomedical Journal Vol 7, No 1 (2015)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v7i1.19

Abstract

BACKGROUND: Acute myocardial infarction (AMI) is one of cardiovascular diseases with high morbidity and mortality rates. Novel biomarkers that can detect accurately acute coronary syndrome (ACS) at early stage, are necessary to improve current strategies and/or to identify subjects who are at risk. Fibrinogen and high-sensitivity C-reactive protein (hs-CRP) roles in inflammation process could be potential for ACS early detection. This study was conducted to evaluate measurements of fibrinogen and hs-CRP on ACS.METHODS: An analytic observational study with cross sectional approach was conducted on patients with Troponin I positive. After signing informed consent, anamnesis and complete blood count were conducted. Besides that, liver function, renal function, and blood glucose tests were conducted as well. Samples of selected subjects were quantified with enzyme-linked immunosorbent assay (ELISA) for Troponin I, fibrinogen and hs-CRP. Then statistical analyses were performed.RESULTS: There were 76 subjects in each ACS and non-ACS groups. ACS group showed significant higher levels of both fibrinogen and hs-CRP compared to Non-ACS group (p=0.000). Among evaluated risk factors, diabetes mellitus (DM) (p=0.003) and hypertension (p=0.000) were significantly higher in ACS group than in non-ACS group. Among evaluated clinical factors, blood glucose (p=0.001) and age (p=0.000) were significantly higher in ACS group than in non-ACS group. Combination of fibrinogen and hs-CRP measurements showed the highest sensitivity (75.00%), specificity (80.26%), accuracy (77.63%), positive predictive value (79.19%) and negative predictive value (76.25%).CONCLUSION: Since fibrinogen and hs-CRP were increased in ACS group and combination of fibrinogen and hs-CRP measurements showed the highest sensitivity, specificity, accuracy, positive predictive value and negative predictive value, we suggest that combination of fibrinogen and hs-CRP measurements could give added value to identify ACS.KEYWORDS: fibrinogen, hs-CRP, biomarker, ACS, acute coronary syndrome, atherosclerosis, inflammation
Conditioned Media of Human Umbilical Cord Blood Mesenchymal Stem Cell-derived Secretome Induced Apoptosis and Inhibited Growth of HeLa Cells Ferry Sandra; Janti Sudiono; Elina Ardiani Sidharta; Elisabeth Pricilia Sunata; Dea Jane Sungkono; Yanni Dirgantara; Angliana Chouw
The Indonesian Biomedical Journal Vol 6, No 1 (2014)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v6i1.44

Abstract

BACKGROUND: Secreted factors contained in conditioned media (CM) of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) known as secretome, was suspected to have important roles in regulating cells. This study was conducted to investigate the role of CM-hUCB-MSCs-derived secretome in apoptosis and growth of HeLa cells.METHODS: HeLa cells were treated with secretome in various concentrations (0, 0.2, 2 and 20%) for 24 and 48 hours. Trypan blue exclusion assay was performed to detect cell viability. Meanwhile sub-G1 apoptotic assay was performed to detect apoptotic cells. The transition of mitochondrial transmembrane potential (TMP), which occurs in the apoptotic process, was analyzed by mitochondrial membrane potential (ΔΨM) assay. Both sub-G1 and ΔΨM assays were performed using FACSCanto flow cytometer. Statistical analyses were conducted using IBM SPSS Statistics to detect significance level at p<0.05.RESULTS: Secretome significantly induced cell death starting at concentration of 0.2% within a 24-hour period (p<0.05). Secretome significantly induced cell death in concentration and time dependent manner (p<0.05). The cell death was then confirmed as apoptosis through sub-G1 analysis. Due to the underlying apoptotic mechanism, we found distinct decrease of TMP, indicating an increase in mitochondrial membrane permeability of HeLa cells. In addition, we found that HeLa cell growth was inhibited partially by secretome.CONCLUSION: Taken together, we conclude that CMhUCB-MSCs-derived secretome significantly induced apoptosis of HeLa cells in a concentration and time dependent manner through mitochondrial apoptotic pathway. The secretome might also play important role in inhibiting HeLa cell growth.KEYWORDS: umbilical cord blood, mesenchymal stem cell, secretome, apoptosis, growth, cancer
Co-Authors Abdul Gani Soulisa Abdul Gani Soulisa Achmad Guntur Hermawan Suryo Adji Achmad Kemal Harzif Addin Trirahmanto Ade Prijanti Dwisaptarini Adriana Todingrante Afiana Rohmani Ahmad Dian Wahyudiono Ahmad Kurnia Ahmad R. Utomo Ahmad R. Utomo Aida Fitri Nazillah Al Mazida Fauzil Aishaqeena Alfred Pakpahan, Alfred Ali Sulaiman Alida Roswita Harahap Alpha Fardah Athiyyah Amalia Isnaeni, Nur Amelia Astriani Putri Aminuddin Aminuddin Amirudin Eso Anang Endaryanto Andayani, Lia H. Andi Asadul Islam Andi Wijaya Andi Wijaya Andon Hestiantoro Andri Sutanto Andrian Nova Fitri Andrijono Andrijono Andy Darma Andy Wirahadikusumah Anggraeni, Rezky Angliana Chouw Angliana Chouw Angliana Chouw Angliana Chouw Ani Retno Prijanti Anie Apriani Anna Fuji Rahimah Annisa Amalia Annisa Putri Ariyani Annistri, Savrinda Anwar Santoso Aprisia, Kartika Dwi Ardian Rizal Aria Kekalih Arief Boediono Ariesanti, Yessy Arif Kurniawan Arif Santoso Arif Satria Arimaswti Arimaswati Ariyani, Annisa Putri Armelia Sari Arum Kartikadewi Arya Nur Ihsan Aryani Hayuningtyas, Ria Aryu Candra Asmarinah Asmarinah Ayasha Hajjar Audreyandra Wahid Ayu Rahadiyanti Ayunita Tri Wirattami Aznan Lelo Bambang Sutrisna Bambang Sutrisna Bastian Nova Benyamin Lukito Bethy Suryawathy Hernowo Bianda Claresta Bianda Claresta Blanca Sarizta Boedi Oetomo Roeslan, Boedi Oetomo Boenjamin Setiawan Britanto Dani Wicaksono Budi Utomo Budiono, Bernardus Parish Bukhari, Agussalim Caecilia Caroline Aliwarga Caesary Cloudya P Caesary Cloudya Panjaitan Cahyono Kaelan Camy F. Surjadi Caroline T. Sardjono Caroline Tan Sardjono Caroline Tan Sardjono Chandra Risdian Chandra Risdian Chantika Amardhia Paramita Putri Ciptadhi Tri Oka Binartha Ciptadhi Tri Oka, Ciptadhi Tri Cloudya P, Caesary Cloudya Panjaitan, Caesary Dea Jane Sungkono Denni Joko Purwanto Devi Nisa Hidayati Deviyanti Pratiwi Dewi Liliany Margaretta, Dewi Liliany Dewi Lilyani Margaretha Dewi Ranggaini Dewi, Dian Andriani Ratna Dhaniar, Afifah Yumna dharma Lindarto, dharma Diah Savitri Ernawati Dian R Laksmitawati Dian Ratih Laksmitawati Diana Nur Afifah, Diana Nur Dina Keumala Sari Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Djoko Wahono Soeatmadji Dominica Dian Saraswati Sumantri Dwi Davidson Rihibiha Dwifulqi, Hernindya Edi Handoko Eka Seftiana Indahsari Elina Ardiani Sidharta Elisabeth Pricilia Sunata Elline, Elline Ellyza Nasrul Elvita Rahmi Daulay Em Yunir, Em Enny Probosari Enos Tangke Arung, Enos Tangke Ergia Latifolia Eriawan Agung Nugroho, Eriawan Agung Erizal Sugiono Erlina Sih Mahanani, Erlina Sih Ervi Afifah Fahriza Abid Sonia Farhat, Farhat Farid Sastra Nagara Farid Sastra Nagara, Farid Sastra Fatmawati Fatmawati Fauzia Purnamasari Febriyeni, Febriyeni Felix Wong Fibryanto, Eko Fillah Fithra Dieny Fitriasih Fitriasih Fitriyadi Kusuma Franciscus D. Suyatna Frans D. Suyatna Frisca Frisca FX Budhianto Suhadi George Mathew Gita Pratama Gwendy Aniko Hadrian, Gary Halim, Johni HANA RATNAWATI Hariyono Winarto Harry Akza Putrawan Harry Murti Harryanto Wijaya Harryanto Wijaya Hartono, Tiffany Harun Iskandar Hayatun Nufus Hayuningtyas, Ria A. Hayuningtyas, Ria Aryani Hendradi Surjotomo Hendy Setyo Yudhanto Heri Wibowo Herqutanto Herqutanto Hidajat, Boerhan Hilary Limen Huda Shalahudin Darusman I Gusti Made Reza Gunadi Ranuh I Putu Sudayasa Ibnu Suryatmojo Ichwan, Solachuddin Jauhari Arief Ida Parwati Idrus Alwi Ihsan Rizal, Muhammad Ikhsan Nur Arifin Ilham Jaya Patellongi Ilhamjaya Patellongi Indra Bachtiar Indradewa, Rhian Indrayadi Gunardi Indriyanti Rafi Sukmawati Indriyanti Rafi Sukmawati Ingrid Suryanti Surono Intan Masyfufah Hanim Irawan Yusuf Irawan Yusuf Irianiwati Widodo Irvan Septrian S P R Irvanyuni Japutri Ivet Suriapranata Jackson Jackson, Jackson James Handojo Jamhari Jamhari Janti Sudiono Janti Sudiono Janti Sudiono Janti Sudiono Janti Sudiono Jasmine Shafira Jasmine Shafira, Jasmine Jeanne A. Pawitan Jeanne A. Pawitan Jenifer Christy Hadimartana Jennifer Putri Jessica Nathalia Chandra Jessica Nathalia Chandra, Jessica Nathalia Joceline Setiawan Johan Arief Budiman Juliani Hardja Buntara, Magdalena Juminten Saimin Junita Briskila Jusak Nugraha Jusuf Rachmat Karina Febriani Hudono Karyono Mintaroem Kent Wijaya Setiawan Ketherin Ketherin Ketherin Ketherin Ketherin Ketherin Ketherin, Ketherin Khairun Nisa Kharima Abdullah Kharima Abdullah, Kharima Khilmi Ainun Nadliroh Kirana, Farenza Okta Komariah Komariah Komariah Komariah Komariah Komariah Kusuma, Yohanna Laifa Annisa Hendarmin Laila Nuranna Lee, Kyung H. Lee, Kyung Hoon Lelyana, Shelly Leo Alberto Porjo Lia Hapsari Andayani Lidya Imelda Laksmi Linda Lison Linda Sari Sembiring Livia Alvita Liza Meutia Sari Louise Anastasya Luki Astuti Luthfiatul Khusna M Novo P Lubis Maesaroh Maesaroh Makmun, Armanto Mansyur Arif Mansyur Arif Marcellus Simadibrata Maria Celinna Maria Celinna Maria Evi Novianti Maria Verena Emeralda Marshel Tendean Marshel Tendean MARTHA ARDIARIA Maulanisa, Sinta C. Maurin Merlina Meita Hendrianingtyas Melanie S Djamil Melanie Sadono Djamil Melanie Sadono Djamil Melanie Sadono Djamil Melanie Sadono Djamil, Melanie Sadono Melina Setiawan Melinia Melinia Meta Ariyani Sidharta Meta Ariyani Sidharta, Meta Ariyani Mirta Hediyati Reksodiputro Mohammad Aris Widodo Mohammad Aris Widodo Mohammad Sadikin Mohammad Sadikin Monica Andajana Muhamad Rizki Fadlan Muhammad Diah Muhammad Hamdan Muhammad Ihsan Rizal, Muhammad Ihsan Muhammad Novo Perwira Lubis Muharam, Muharam Muharam, Raden Mulyawati, Sufiah Asri MUTMAINNAH, FITRI Nadhia Sari Afiana Nadhia Sari Afiana, Nadhia Sari Nadiah, Najla Nadya Saputri Halim Najla Nadiah Naliani, Silvia Nani Maharani Nanik Setjowati Nasrul Zubir Neni Susilaningsih Nenni Pratiwi Nova Adrian Noviasti Rahma Utami Novriansyah, Robin Nur Ahmad Tabri Nur Indah Purnamasari Nur Zam Zam Nuralifah Nuralifah, Nuralifah Nurani Hayati Nuri Dyah Indrasari Nurjannah Lihawa Nurrani Mustika Dewi Nurrani Mustika Dewi Nurrani Mustika Dewi, Nurrani Mustika Nurul Fauziah Nyoman Suci Widiastiti Oetomo Roeslan, Boedi Olivia Nauli Komala P Lubis, M Novo Pande Putu Erawijantari Pang, Tiffany Parawansah Parawansah Pendrianto Pendrianto Peter Kabo Peter Sugita Pieri Kumaladewi Prasetya, David Eka Pratitis, Visi Endah Primariadewi Rustamadji Primariadewi Rustamadji Pudji Rahajoe Pudji Rahaju Pukovisa Prawiroharjo, Pukovisa Putri Y Suyanto Putri Y Suyanto, Putri Y Putri, Indah Aprianti Quan Yong Tang Rachma Purwanti Rafika Indah Paramita Rahmawati Rahmawati Rahmawati, Dicha Yuliadewi Rahmi Amtha Rahmi Amtha Rahmi Annisa Ramadhan Karsono Ramadhan Ramadhan Rambu Beppy Hamuaty Rambu Beppy Hamuaty, Rambu Beppy Ranggaini, Dewi Ratna Akbari Ganie Respati, Danendra Rakha Putra Retno Asih Setyoningrum Reynaldo Lambertus Rifqi Fauzan Hakim Riko Nofrizal Rio Auricknaga Kintono Riris L. Puspitasari Riris L. Puspitasari Rita Lahirin Rita Lahirin, Rita Ritawaty Ritawaty Rizal Rizal Rizkia Milladina Hidayatulloh Roihatul Mutiah Ronald Winardi Kartika Rosalina Tjandrawinata Roya Khosravi-Far Roya Khosravi-Far Ryco Giftyan Ardika Saleha Sungkar Saptawati Bardosono Saraswati, Jaya Sari, Armelia Sarwono Waspadji Scania, Alifah Evi Seila Arumwardana Septrian S P R, Irvan Setiawan, Kent Wijaya Siti Mega Komariyah SITI NURUL HIDAYATI Soehartono Soehartono Solachuddin Jauhari Arief Ichwan Sonar Soni Panigoro Soulisa, Abdul Gani Soulissa, Abdul G. Sri Mutya Sekarutami Stefani, Rosita Steffi Kurniawan Steward Hadi Steward Steward Sudarmo, Subijanto Marto Suhendro Suhendro Sukartini, Ninik Susi Tjahyani Susy Tjahyani Suzzana Immanuel Syafiqa Amelia, Dinda Syafira Noor Pratiwi Syakib Bakri Syanindita Puspa Wardhani Syarifin, Andi Noor Kholidha Tadahiko Iijima Tadahiko Iijima Taramalinda, Elizabeth Yuliani Taslim, Nurpudji Astuti Tatsushi Muta Teguh Santoso Teguh, Sharren Timotius Andi Kadrianto Tjandra, Kevin Christian Tjandrawati Mozef Tjandrawati Mozef Tjandrawati Mozef Todung Silalahi Toshio Kukita Toshio Kukita Trijani Suwandi Trisfilha, Pretty Tugas Ratmono Tulandi, Togas Uleng Bahrun Victor Reiner Sutanto, Aldrich Virgi Saputra Wa Ode Siti Rahayu Fathanah WAHYU WIDOWATI Wahyu Widowati Wahyu Widowati Wahyu Widowati Wibi Riawan Widiastiti, Nyoman Suci Widya Wulandari Widyastuti, Wiena Wiena Widyastuti Wijaya, Haryanto William Yusuf, Timotius Winarko Luminturahardjo Wirsma Arif Harahap Yanni Dirgantara Yanni Dirgantara Yanni Dirgantara Yanuarita Tursinawati Yefta Moenadjat Yenti Purnamasari Yohanna Feter Yohanna Feter, Yohanna Yora Nindita Yudi Her Oktaviono, Yudi Her Yuliani Taramalinda, Elizabeth Yusra Yusra Yusrina Lukitasari Yuyus Kusnadi Zulfikar Lubis Zulfikri Muhktar