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Perbedaan Jumlah Sel Neuron Cerebrum dan Cerebellum Mus musculus pada Kehamilan Remaja dan Dewasa Tri Purwanti; Widjiati Widjiati; Muhammad Miftahussurur
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 9, No 1 (2020): MARET 2020 available online since April 2020
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.515 KB) | DOI: 10.30742/jikw.v9i1.639

Abstract

Teenage pregnancy contributes to emotional stress higher than adult women Pregnancy in adolescents will trigger negative thoughts and feelings of fear that become the root cause of stress reactions. The onset of stress will trigger the occurrence of Axis HPA activity and the release of corticotrophin releasing hormone (CRH) by the paraventricular nucleus of the hypothalamus, then stimulate the production of adrenencorticotropic hormone (ACTH) by the anterior pituitary gland. ACTH will stimulate glucocorticoids (cortisol) from the adrenal gland cortex to increase the production of CRH in the placenta and give an effect of increasing cortisol in the maternal, as well as the amount of cortisol in the fetus will also increase because it follows the blood placenta barrier. This affects the growth and development of the fetal brain, so that the process of proliferation and differentiation, migration, organization and synaptogenesis and myelination in brain cells. The growth of the brain decreases which affects the number of neuron cells. This study aims to analyze the differences in the number of neuron cells in the cerebrum and cerebellum Mus musculus newly born in adolescent and adult pregnancy. The division of the study group consisted of two groups, namely the adolescent and adult pregnancy groups each of 16 individuals. Taking the examination sample is by taking each of the 3 children from the parent with the heaviest, medium and lowest weights. Then the Mus musculus children were sacrificed by anesthesia and decapitation, then Hematoxilin-Eosin preparations were made from the child's brain. The next step is to examine Hematoxilin-Eosin to calculate the number of neuron cells in the cerebrum and cerebellum with the analysis of the Independent T test showing significant differences between the control and treatment groups with a value of p = 0,000 (p <0.05). Then the analysis of the number of brain neuron cells using the Mann Whitney test showed a difference that the control group was higher than the adolescent group.
IDENTIFIKASI GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) DARI MATURASI IN VITRO OOSIT SAPI DENGAN TEKNIK IMUNOSITOKIMIA Widjiati w; Desi Wulansari; Wurlina w; Ngakan Made Rai Widjaja
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.57 KB) | DOI: 10.21157/j.ked.hewan.v5i2.356

Abstract

Penelitian ini bertujuan mengindentifikasi GDF-9 di dalam oosit yang berasal dari preantral folikel sapi yang dimatangkan secara in vitro. Penelitian ini menggunakan metode imunositokimia ikatan antara molekul avidin dan biotin yang terkandung dalam antibodi sekunder bentuk kompleks avidin-biotin. Ovarium sapi diambil dari rumah potong hewan (RPH) Pegirian Surabaya. Penelitian ini menggunakan 105 oosit matang yang berasal dari 62 ovarium. Hasil imunositokimia menunjukkan bahwa 77,14% GDF-9 teridentifikasi.
THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Dhesy Kartikasari; Sri Mulyati; Suzanita Utama; Pudji srianto; Widjiati Widjiati; Hani Plumeriastuti
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.285 KB) | DOI: 10.21157/j.ked.hewan.v14i1.15283

Abstract

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.
IDENTIFIKASI PROTEIN EPIDERMAL GROWTH FACTOR (EGF) 46 kDa HASIL MATURASI OOSIT SAPI SECARA IN VITRO Widjiati w; Anike Rachmawati; Sri Mumpuni; Bambang Sektiari
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.059 KB) | DOI: 10.21157/j.ked.hewan.v6i1.734

Abstract

Tujuan dari penelitian ini adalah mengidentifikasi suatu protein dengan berat molekul 46 kDa yang diduga sebagai epidermal growth factor (EGF) yang diisolasi dari oosit sapi yang telah dimaturasi secara in vitro dengan metode elektroforesis. Ovarium sapi yang berasal dari rumah potong hewan, diaspirasi pada folikel dengan diameter permukaan ≤5 mm menggunakan spuit dan jarum. Oosit dimaturasi dalam tissue culture medium (TCM) 199 selama 22 jam pada suhu 38,5° C di dalam inkubator CO2. Preparasi protein dengan berat molekul 46 kDa menggunakan sodium dodecyl sulphate polyacrilamide gel elektroforesis (SDS PAGE). Berdasarkan perhitungan jumlah regresi dari protein marker, didapatkan 12 fraksi protein yaitu BM 172,7; 153,09; 118,24; 102,43; 89,27; 59,75; 46,41; 43,82; 40,2; 36,06; 23,45; dan 18,42 kDa. Protein dengan berat molekul 46,41 kDa yang tampak pada pita protein dapat diidentifikasi sebagai protein yang diduga EGF yang berperan dalam proses maturasi oosit.
EFEKTIVITAS TERAPI RAT BONE MARROW MESENCHYMAL STEM CELL PADA TIKUS (Rattus norvegicus) MODEL TERATOGENIK PARTICULATE MATTER TERHADAP EKSPRESI TNF- , BAX, DAN BCL-2 PLASENTA Sri Pantja Madyawati; Rimayanti R; Widjiati W; Agung Budianto Achmad
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.642 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2774

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Tujuan penelitian ini adalah membuktikan potensi dan efektivitas rat bone marrow mesenchymal stem cell (rbmmsc) sebagai terapi pada kasus teratogenik selama kebuntingan dengan melihat ekspresi tumor necrosis factor (TNF-α), Bax, dan Bcl-2. Dua puluh empat ekor tikus bunting model teratogenik dibagi menjadi empat kelompok perlakuan yang berbeda yaitu P1-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + Injeksi MEM 0,1 ml), P1-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml), P2-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-17 + Injeksi MEM 0,1 ml), P2-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umurkebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml). Data yang didapatkan kemudian dianalisis menggunakan uji MannWhitney. Hasil penelitian ini menunjukkan bahwa pemberian terapi rat bone marrow mesenchymal stem cell pada tikus model teratogenikparticulate matter tidak berpengaruh dalam menurunkan parameter ekspresi TNF-α dan Bax, serta meningkatkan ekspresi Bcl-2 pada plasenta.
Hubungan teknik pemerahan dengan jumlah Escherichia coli pada susu segar dari peternakan sapi perah di KUD Kertajaya, Kabupaten Kediri, Jawa Timur Rahadyan, Raihan Alif; Tyasningsih, Wiwiek; Puspitasari, Yulianna; Permatasari, Dian Ayu; Widjiati, Widjiati
Current Biomedicine Vol. 1 No. 1 (2023): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.1.19-24

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Background: The coliform bacteria group is a group of bacteria used as an indicator of food sanitation, one of which is in dairy products. Objective: This study aimed to determine the relationship of milking techniques with the number of Escherichia coli in raw milk compared to the bacterial contamination limit set by the National Standardization Agency. Methods: The design of this study was a descriptive study. Raw milk samples were taken from Lucky Farm and several traditional dairy farms in the KUD Kertajaya area, Kediri Regency, East Java. This study uses the most probable number (MPN) test. Results: The results showed 11 (73.3%) of raw milk samples using conventional milking techniques exceeding the maximum limit of E. coli bacterial contamination determined by the National Standardization Agency (< 3 MPN/mL). However, all 15 samples, or 100% of raw milk samples using machine milking techniques, were negative for E. coli and met the maximum bacterial contamination limit requirements. Conclusion: The milking techniques affect E. coli contamination limits in raw milk. The milk samples taken using machine milking techniques had better results with MPN values ​​< 3 MPN/mL than those taken using conventional milking techniques.
Pengaruh Paparan Uap Rokok Elektrik Terhadap Gambaran Histopatologi Organ Pulmo Tikus Putih (Rattus norvegicus) Ardy, Carolina; Hamid, Iwan Sahrial; Yudhana, Aditya; Widjiati, Widjiati
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (913.565 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.38-44

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Studi ini bertujuan untuk mengidentifikasi pengaruh paparan uap rokok elektrik terhadap gambaran histopatologi organ pulmo. Tikus putih yang digunakan sebanyak 20 ekor yang terbagi menjadi 5 kelompok, kontrol (K) adalah yang tidak diberikan paparan uap rokok elektrik dan kelompok perlakuan yang diberikan paparan uap dengan dosis yang berbeda, kelompok Perlakuan I (0,3 mg/ml), P2 (3 mg/ml), P3 (12 mg/ml), P4 (36 mg/ml). Setelah 14 hari perlakuan, tikus putih dieuthanasia dan dinekropsi serta organ pulmo diawetkan untuk dilakukan pembuatan preparat histopatologi dengan menggunakan pewarnaan Hematoxilin-Eosin. Hasil menunjukkan terdapat kerusakan pada gambaran histopatologi pulmo yang diamati pada perbesaran 400x. analisis data menggunakan Kruskal-Wallis dan dilanjutkan dengan uji Mann Whitney menunjukkan perbedaan yang signifikan (p<0.05). Rata-rata kerusakan organ pulmo tikus putih adalah kontrol (K)=3.00; (P1)=6.00; (P2)=12.00; (P3)=13.00 dan (P4)= 18.50 dan dosis minimal dari nikotin adalah 3 mg/ml. Kesimpulan, paparan uap rokok elektrik dapat menyebabkan kerusakan yang signifikan pada organ pulmo tikus putih.
Protective Effects of Apis Dorsata Honey Supplementation on Kidney Histopathology in Mice with Monosodium Glutamate Exposure Rao, Sherbrina Bai Seenivasa; Widjiati, Widjiati; Lokapirnasari, Widya Paramita; Triakoso, Nusdianto; Safitri, Erma; Kuncorojakti, Suryo; Proboningrat, Annise
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.63-69

Abstract

This study was conducted to investigate the protective effects of Apis dorsata honey on the histopathological changes of the kidney in mice exposed to monosodium glutamate. This study used 25 male mice as the experimental animal which were divided into five groups with five replication, respectively i.e. (C-) was administered basal diet, (C+) was administered 4 mg/g BW MSG, (T1) was administered A. dorsata honey of 53.82 mg/20g BW followed by MSG 4 mg/g BW, (T2) was administered A. dorsata honey of 107.64 mg/20g BW followed by MSG 4 mg/g BW, and (T3) was administered A. dorsata honey of 161.46 g/20g BW followed by MSG 4 mg/g BW. All treatments were carried out orally for 52 days. This study was observed tubular epithelial hydropic degeneration, epithelial necrosis, and glomerular necrosis. Data were analyzed using Kruskal-Wallis test followed by the Mann-Whitney test. As a result, for the epithelial and glomerular necrosis variables, the T3 group showed significant results when compared with C+, T1, and T2 groups. Moreover, T3 was not significantly different from the C- group. It can be concluded that A. dorsata honey in T3 group could protect mice kidneys from the damaging effect of MSG.
POTENSI KANDUNGAN ANTIOKSIDAN RUMPUT KEBAR (Biophytum Petersianum Klotzsch) TERHADAP APOPTOSIS DAN GAMBARAN HISTOPATOLOGIS TESTIS PADA MENCIT (MUS MUSCULUS) YANG DIPAPAR 2,3,7,8-Tetrachlorodibenzo-p-dioxin Dewita, Dewita; Widjiati, Widjiati; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Hariadi, Mas'ud; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19986

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic compound in the dioxin group. This compound is a pollutant for the environment and very harmful to human health and enter the body through the mucous membranes in the mouth and the respiratory tract and can be transmitted through the placenta and lactation. The aim of this study was to know the antioxidantpotency of to resolve reproduction disturbance caused by TCDD exposure.Thirty Balb/C male mice were divided into five different groups, the negative control group, a positive control group exposed to TCDD at a dose of 7 μg/kg BW, P1 group of groups exposed to TCDD doses of 7 μg / kg BW and given Biophytum petersianum extract 0.05mg/gBB/day, group P2 group exposed to TCDD doses of 7μg/kgBW and given 0.080mg/gBB/day, and group P3 were exposed to TCDD dose of 7μg/kgBB and given the extract of Biophytum petersianum 0.135mg/gBB/day during day 2 to day 55. On the 56th day the mice were sacrificed and apoptotic examination and spermatogenic cell histopathological features were performed on the testis. The results showed that: P2 (0,433 ± 0,497; p<0,05) and P3 (0,200 ± 0,000 p<0,05) groups were the most effective group in decreasing spermatogenic cell apoptosis compared to positive control group (2,933 ± 1,5832 p<0,05). The Johnsen score result showed that P2 (9,400 ± 0,420) and P3 (9,800 ± 0,253) groups improving the histopathologic picture of spermatogenic cells in seminiferous tubules compared to positive control group (7,20 ± 0,400) p<0,05. Conclusion of this study were Biophytum petersianum is effective to solve reproduction disturbances caused by exposure of TCDD and the P3 group is the most effective group.
DESCRIPTION OF HEMATOLOGICAL VALUE IN LEOPARD GECKO (Eublepharis macularius) Wiranata, Try Ardhi; Widjiati, Widjiati; Wahyuni, Retno Sri; Hidajati, Nove; Poetranto, Emmanuel Djoko; Yuliani, M. Gandul Atik
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19990

Abstract

The aim of this research was to explore the hematological value of leopard gecko (Eublepharis macularius). Total of 20 samples were examined, its consists of 10 males and 10 females, blood samples were taken from vena ventral abdominal and deposited into EDTA tubes, fresh blood leaves little to make blood smear, total blood examination conducted with automatic hematological analyzer ABX MICROS 60 and for total leukocyte and differential leukocyte count used standard manual method. The mean result of hematological value of leopard gecko in this research are WBC (x103/mm3) 7,12 male, 8,09 female, Lymphocyte (%) 46,2 male, 38,4 female, Monocyte (%) 17 male, 17,3 female, Heterophil (%) 18,3 male, 21,8 female, Eosinophil (%) 10,8 male, 13,9 female, Basofil (%) 7,7 male, 8,6 female, RBC (106/mm3) 0,64 male, 0,65 female, HGB (g/dl) 8,95 male, 9,46 female, Hematocrit (%) 10,55 male and 9,26 female. In all parameters showed that hematology value of male gecko leopard was lower than female gecko except on two parameters which are hematocrit and lymphocyte which showed male gecko has the higher value than females, but the hematology value difference of both sexes leopard gecko is not significant except in heterophil value. 
Co-Authors . AULANI’AM Achmad Salman Achmad Salman Faridz, Achmad Salman Achmad Yunus Adi Setyawan Prianto Agung Budianto Achmad, Agung Budianto Agus Sulistyono Agustina Hidayati Agustina Mar&#039;atus Sholichah Agustina, Widayati Ahmad Rukhani Lutfi Alfiah Hayati Ali Madinah Ali Medina Ali Medina Aliyah, Suhartatik Amila, Ukhti Mukminah ilmi Ancha Ayu Amishinta Andita Hapsari Anike Rachmawati Anike Rachmawati Anindya Marcha Angelina Djianto Anna Surgean Veterini Annis Catur Adi Annise Proboningrat Anwar Fauzi Ardy, Carolina Arief Boediono Arimbi Askandar Tjokroprawiro Aswin, R. Haryanto Aty Widyawaruyanti Aucky Hinting Ayu Putri Yani Azami D Azinar Bambang Poernomo Bambang Purwanto Bambang Sektiari Lukiswanto Bambang Wahjuprajitno Berlina, Cyrcilia Relita Binta Dwi Novitasari Boedi Setiawan Budi I Santoso Budi Prasetyo Budi Santoso Budi Santoso Budi Santoso Budi Susetyo Pikir Budi Susetyo Pikir Budi Utomo Budi Utomo Budi Wicaksono Budi Wicaksono Chairul Anwar Darsono, Roesno Desak Ketut Ayu Aryani Desi Wulansari Desi Wulansari Dessy Hidayati Fajrin Dewita Dewita Dhesy Kartikasari Dian Sukmawati Diana Estu Rumahastuti Dina Helianti Dwi Gustin, Mandasari Dwi Nurdi Puspita Sari Dwi Puji Wijayanti Eighty M Kurniawati Eighty Mardiyan K, Eighty Eka Nasrur Maulana Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Setyaningsih endra, priawasana Epy Muhammad Luqman Erma Safitri Ermawati Ermawati Erry Gumilar Dachlan, Erry Gumilar Dachlan, Erry Gumilar Exma Mu&#039;tatal Hikmah Faizah, Zakiyatul Fauzan Djunaidi Fedik A Rantam Fedik Abdul Rantam Fitria Desky Gandul Atik Yuliani Gatut Hardianto, Gatut H.M Bahkriansyah Hamdani Lunardhi Hana Eliyani Hani Plumeriastuti Hany Aryani Puspita Hari Basuki Notobroto Hari Paraton Hariadi, Mas'ud Harry Mangasi Binsar Panjaitan Haspita Rizki Syurya Handini Hendy Hendarto HENI RACHMAWATI HERAWATI, LILIK Herlina Alvianti N Herlina Puji Angesti Hermanto Hermanto Hermanto Hermanto Hermanto T Joewono Hermanto TJ Hermanto Tri J Hermanto Tri Joewono Hermanto Tri Juwono Heru Santoso Wahito Nugroho Hidajati, Nove Hidayatullah, Amiruddin Ika WAHYUNI Imam Mustofa IMAM MUSTOFA INDRA YULIATI, INDRA Inu Mulyantoro Iwan Sahrial Hamid Jeanita, Winny Jimmy Yanur Annas Jojor Sihotang Joseph Chandra Relmasira Kartikasari, Dhesy Khasanah, Rima Nur Kuncorojakti, Suryo Kurniawati, Sepvian Dewi Kusumawardani, Emi Laba Mahaputra Laili Muninggar Laksmi Sulmartiwi Lilik Maslachah Machmudia, Ainun Mafruchati, Maslichah Masyitah Hamidah Meitria Syahadatina Noor Meles, Dewa Ketut Mikra Latisfian Mikra Latisfian Mikra Latisfian Muhammad Miftahussurur Mulyanto Mulyanto Mulyanto Mulyanto Muninggar, Laili MY Ardianta W N Cininta M Nasrul Wahdi Nenny Harijani Ngakan Made Rai Widjaja Ngakan Made Rai Widjaja Ningrum, Nyna Puspa Noor Pramono Noor Pramono Noor Pramono Noor Pramono Noerpramana Nur Anindya Syamsudi Nur AR Widiatmoko Nur Cholila Nur Laila Faizah Nurbayatin, Anita Nursadida, Milla Nurvy Alief Aidillah Nusdianto Triakoso Nyna Puspa Ningrum Oktoria Indrapraja Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Liben Paulus Sugianto, Paulus Permatasari, Dian Ayu Pipit Sri Estuning Rahayu Pitria Permatasari Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Pudji Srianto Rachmad Suhanda Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Ratriana Via Parasti Raudatul Hikmah Rehatta, Nancy Margarita Relly Yanuari Primariawan, Relly Yanuari Rimayanti R Rimayanti R, Rimayanti Rimayanti Rimayanti RINA YUDIWATI BM, RINA Risna Y, Dyah Ayu Roro Rochmah Kurnijasanti Romi Ermawan Romi Ermawan, Romi Rusyawardani, Aldis Ingrid S.Pd. M Kes I Ketut Sudiana . Salmon Charles Pardomuan Tua Siahaan Sami, Abdul Sari, Gadis Meinar Sarmanu, Sarmanu Sarudji, Suryanie Seindy Glamour Shabira, Zahra Siti Nur Kholifah Siti Nur Kholifah Siti Nur Kholifah SOETJIPTO Sofiyanti Miftakhurohmah Sri Mulyati Sri Mulyati Sri Mumpuni Sri Mumpuni Sri Pantja Madyawati Sri Pantja Madyawati, Sri Sri Pantja Madyawati, Sri Pantja Sri Ratna Dwiningsih Subijanto Marto Soedarmo Suherni Susilowati Sulistiawati Sulistiawati Sutiman Bambang Sumitro Suwarno . Suzanita Utama Syarief T. Hidayat Syarief Thaufik Hidayat Syifa Fauziyah Tias, Eriza Putri Ayu Ning Tinta Julianawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Hartini Yuliawati Tri HS Hadi Tri Purwanti Tri Wahyu Suprayogi Trilas Sardjito TRINIL SUSILOWATI Tyasningsih, Wiwiek Ukhti Mukminah ilmi Amila Varianida Veterini Virki Widoyanti Viski Fitri Hendrawan Wahyuni, Retno Sri Wicaksono, M Galih Widodo Jatim P Widya Paramita Lokapirnasari Windhu Purnomo Wiranata, Try Ardhi Wurlina, W Yaner, Nurul Ramadhani Yeni Dhamayanti Yoes Priyatna Dachlan Yudhana, Aditya Yudi Her Oktaviono Yudi Her Oktaviono, Yudi Her Yuli Trisetiyono Yulia Putri Permatasari Yulianna Puspitasari