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Pertumbuhan cacing tanah Eisenia fetida sp. Pada kompos limbah fleshing Prayitno, Prayitno
Majalah Kulit, Karet, dan Plastik Vol 31, No 2 (2015): Majalah Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.009 KB) | DOI: 10.20543/mkkp.v31i2.499

Abstract

The use of earthworm as a source of income and a means for managing organic solid waste such as fleshing waste has been widely applied. The aim of this research was to find the optimum ratio between fleshing waste and cow dung in the growing medium of Eisenia fetida sp. This research was conducted by growing the E. fetida sp. earthworm in the medium containing fleshing waste mixed with cow dung. The ratio of fleshing waste and cow dung was varied at 0:100; 10:90; 20:80; 30:70; 40:60; and 50:50. In addition, 2 parts of chopped stubbles per part of every sample was added as a carbon source. The prepared media were fermented for three weeks with EM4 as a starter, followed by incubation of the earthworm for six weeks. The weight and the number of earthworm were evaluated every two weeks. The optimum growth of earthworm was achieved at the 2nd week of incubation with 185.48% and 121.10% increase of weight and number of earthworm, respectively, at 40:60 ratio of fleshing waste and cow dung in the growing medium.Keywords: earthworm, fleshing waste, incubation.ABSTRAKPenggunaan cacing tanah sebagai sumber pendapatan dan juga sarana untuk penanganan limbah padat organik seperti limbah fleshing saat ini telah banyak dilakukan. Penelitian ini bertujuan untuk mengetahui kondisi optimal perbandingan antara limbah fleshing dan kotoran sapi untuk pertumbuhan cacing tanah E. fetida sp. Penelitian dilakukan dengan menumbuhkan cacing tanah E. fetida sp. dalam media campuran limbah fleshing dan kotoran sapi dengan variasi perbandingan berturut-turut 0:100; 10:90; 20:80; 30:70; 40:60; dan 50:50 dan sebagai tambahan sumber karbon ditambahkan cacahan jerami sebanyak 2 bagian untuk setiap perlakuan. Semua media perlakuan difermentasikan selama tiga minggu dengan bantuan larutan starter EM4, dilanjutkan dengan inkubasi cacing tanah selama enam minggu. Berat serta jumlah cacing tanah dievaluasi setiap dua minggu. Pertumbuhan optimum cacing tanah dicapai pada minggu ke-2 dengan kenaikan bobot cacing tanah sebesar 185,48% dan jumlah 121,10% pada perlakuan perbandingan limbah fleshing dengan kotoran hewan 40:60.Kata kunci: cacing tanah, inkubasi, limbah fleshing.
Pengurangan nitrogen pada limbah cair terolah industri penyamakan kulit menggunakan sistem wetland buatan Prayitno, Prayitno
Majalah Kulit, Karet, dan Plastik Vol 30, No 2 (2014): Majalah Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (768.282 KB) | DOI: 10.20543/mkkp.v30i2.129

Abstract

Recently constructed wetland have been widely used in wastewater treatment because of its low energy consumption and its simple operation. A research for removing nitrogen in the treated tannery wastewater by using constructed wetland have been conducted. Two unit of constructed wetland were used. Each unit was vegetated with Equisetum hyemale and Echinodorus palaefolius respectively. Both unit were then operated with subsurface flow system by varying the residence time at 3.125; 2.083; and 1.563 days. The results showed that the optimum effectiveness of nitrogen reduction were 83.67% with a residence time of 3.125 days and 70.29% with a residence time of 2.083 days for Equisetum hyemale and Echinodorus palaefolius respectively. Nitrogen reduction rate constant of 0.275 by using the formula of the USEPA was only suitable for wetland with Equisetum hyemale with R2 of 0.880.Keywords: constructed wetland, nitrogen removal, Equisetum hyemale, Echinodorus palaefolius.ABSTRAKSaat ini wetland buatan sangat luas digunakan untuk pengolahan limbah cair dikarenakan rendahnya energi yang diperlukan dan pengoperasiannya yang sederhana. Penelitian untuk penurunan kadar nitrogen limbah industri kulit terolah dengan menggunakan wetland buatan telah dilakukan. Penelitian dengan menggunakan dua unit wetland buatan dimana masing-masing ditanami dengan tumbuhan bambu air dan melati air. Kedua unit wetland buatan dioperasikan dengan sistem aliran di bawah permukaan dengan memvariasi waktu tinggal berturut-turut 3,125; 2,083; dan 1,563 hari. Hasil penelitian menunjukan bahwa efektifitas optimum penurunan nitrogen untuk unit wetland dengan tanaman bambu air adalah sebesar 83,67% dengan waktu tinggal 3,125 hari, sedangkan untuk melati air penurunan nitrogen optimum terjadi pada wetland dengan waktu tinggal 2,083 hari dengan efektifitas penurunan sebesar 70,29%. Konstanta kecepatan penurunan nitrogen sebesar 0,275 dengan menggunakan formula dari USEPA hanya sesuai untuk wetland dengan tumbuhan bambu air dengan R2 sebesar 0,880.Kata kunci: wetland buatan, pengurangan nitrogen, bambu air, melati air. 
Pengurangan COD dan BOD limbah cair terolah industri penyamakan kulit menggunakan taman tanaman air dengan tanaman melati air Prayitno, Prayitno
Majalah Kulit, Karet, dan Plastik Vol 29, No 1 (2013): Majalah Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.327 KB) | DOI: 10.20543/mkkp.v29i1.217

Abstract

ABSTRACTA research was performed to treat the treated tannery wastewater using mexican swordplant(Echinodorus palaefolius) in constructed wetland. It was designed by uplow system with 3m x 1 m x 1 m in dimension equal with 3 m3 in volume, whereas the media consist of 0.3 m thickfirst layer of rock in the bottom; 0.3 m thick gravel in second layer and 0.1 m third layer of Blacksugar-palm fiber and at the top was sand layer with 0.3 m in thickness. The experiment was run byCOD and BOD loading varied at 0.137; 0.137; 0.392; 0.409; 0.643; 0.648 kg/d for COD and0.049; 0.051; 0.125; 0.133; 0.464; 0.467 kg/d for BOD. Effectivity of constructed wetland wasdetermined by COD and BOD removal. Optimum effectivity of COD removal by 68.75% wasfound by operational condition of influent debit 0.576 m3/d ; detention time 2 days and 2 hours;COD loading 0.405 kg/d, whereas optimum effectivity of the BOD removal by 61.79 % was foundby operational condition at influent debit1.440 m3/d ; detention time 1 days 13 hours and 30minutes and COD loading 0.466 kg/d and reducing constant (k) = 0.008 d-1.Keywords: constructed wetland, treated tannery wastewater, Echinodorus palaefoliusABSTRAKTelah dilakukan penelitian untuk mengolah limbah cair terolah industri penyamakan kulitdengan metode taman tanaman air menggunakan tanaman Melati air (Echinodorus palaefolius).Penelitian ini dilakukan dengan membangun taman tanaman air yang memiliki dimensi panjangx lebar x dalam yaitu 3m x 1m x 1m atau identik dengan volume sekitar 3 m3. Media pengisi tamantanaman air dibuat dari: lapisan paling bawah koral ketebalan 0,3 m, lapisan kedua kerikildengan diameter ketebalan 0,3 m, lapisan ketiga ijuk dengan ketebalan 0,1 m dan lapisan palingatas berupa pasir dengan ketebalan 0,3 m, sistem aliran menggunakan sistem upflow. Tanamanyang digunakan adalah tanaman Melati air (Echinodorus palaefolius var. Latifolius). Perlakuanoperasional dengan memvariasi COD dan BOD loading, berturut-turut untuk COD: 0,137;0,137; 0,392; 0,409; 0,643; 0,648 kg/d sedangkan untuk BOD : 0,049; 0,051; 0,125; 0,133; 0,464 5dan 0,467 kg/d. Efektivitas kerja taman tanaman air diukur dengan penurunan kadar BOD dan 5COD. Efektivitas optimal penggurangan COD limbah yang telah diolah dengan taman tanamanair sebesar 68,75% diperoleh pada perlakuan dengan debit influent 0,576 m3/d, waktu tinggal2,083 hari dengan COD loading 0,405 kg/d, sedangkan untuk pengurangan BOD efektivitas 5pengurangan sebesar 61,79% diperoleh pada perlakuan dengan debit influent 1,440 m3/d, waktutinggal 1,563 hari dengan COD loading 0,464 kg/d, diperoleh konstanta k = 0,008 d-1.Kata kunci: Taman tanaman air, air limbah penyamakan terolah, melati air
Pemanfaatan trimming kulit pikel sebagai flokulan melalui hidrolisis kolagen menggunakan basa untuk penjernihan air Sugihartono, Sugihartono; Sutyasmi, Sri; Prayitno, Prayitno
Majalah Kulit, Karet, dan Plastik Vol 31, No 1 (2015): Majalah Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (513.021 KB) | DOI: 10.20543/mkkp.v31i1.221

Abstract

Wastes in the form of skin derivatives from leather tanning industries remain serious problems if not managed properly and quickly, since they can cause environmental pollution. This research aimed at studying the influence of base type and concentration for the hydrolysis of trimmed collagen from pickled sheepskin to the yield and properties of gelatin, as well as the gelatin’s potential as flocculant. The hydrolysis of trimmed collagen from pickled sheepskin was performed using KOH or NaOH solution, each with varied concentrations of 1, 2, 3, and 4% w/v, for 16 hours. The extraction of the resulting gelatin was performed using water (2.5 water part : 1 waste part), at 70-80 oC, for 3 hours. The results show that the yield of gelatin from hydrolysis using KOH (16.50-28.60%) is lower than that using NaOH (23.68-34.42%). The water and fat contents of the resulting gelatin were relatively similar, while the protein content from KOH treatment was higher than that from NaOH treatment. Hydrolysis with KOH 1% and NaOH 1% and 2% resulted in gelatin that can be used as flocculant. Hydrolysis with NaOH 2% was the best treatment to produce gelatin as flocculant.Keywords: pickled sheepskin, trimming waste, hydrolysis, gelatin,  flocculant.ABSTRAKLimbah berupa kulit turunan dari industri penyamakan kulit masih menjadi masalah serius apabila tidak ditangani secara tepat dan cepat, karena dapat menimbulkan pencemaran lingkungan. Penelitian ini bertujuan untuk mempelajari pengaruh jenis dan konsentrasi basa untuk hidrolisis kolagen hasil trimming kulit domba pikel terhadap rendemen dan sifat gelatin serta kemampuannya sebagai flokulan. Hidrolisis kolagen hasil trimming kulit domba pikel dilakukan dengan menggunakan larutan KOH dan NaOH, dengan konsentrasi berturut-turut 1, 2, 3, dan 4% b/v selama 16 jam. Ekstraksi gelatin dilakukan dengan menggunakan air (2,5 bagian air : 1 bagian kulit) pada suhu 70 – 80 oC, selama 3 jam. Hasil penelitian menunjukkan rendemen gelatin hasil hidrolisis menggunakan KOH adalah sebesar 16,5 - 28,60%, lebih rendah dibandingkan dengan menggunakan NaOH, yaitu sebesar 23,68 - 34,42%. Kandungan air dan lemak gelatin relatif sama, sedangkan kandungan protein hasil perlakuan KOH lebih tinggi dari pada NaOH. Sebaliknya, kandungan abu dan pH perlakuan KOH lebih rendah dibandingkan dengan perlakuan NaOH. Hidrolisis dengan KOH 1% dan NaOH 1% serta 2% menghasilkan gelatin yang dapat berfungsi sebagai flokulan. Hidrolisis menggunakan NaOH 2% merupakan perlakuan terbaik untuk menghasilkan gelatin sebagai flokulan.Kata kunci: kulit domba pikel, limbah trimming, hidrolisis, gelatin, flokulan.
THE INFLUENCE OF STARTER VOLUME AND AIR FLOWRATE IN HOSPITAL WASTE WATER TREATMENT USING AEROBIC FIXED FILM BIOFILTER BATCH (AF2B) REACTOR Prayitno, Prayitno; Saroso, Hadi; Rulianah, Sri; Prastika, Mita
Jurnal Bahan Alam Terbarukan Vol 6, No 1 (2017): June 2017 [Nationally Accredited]
Publisher : Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v6i1.7952

Abstract

BOD, COD, phenol and ammonia-free in the effluent of hospitals wastewater often exceed the quality standards. This was due to less optimal biological processes to degrade the pollutants. So we need an efforts to find optimal process conditions through the engineering process and the factors that affect the biodegradation of pollutants. On the other hand, AF2B reactor containing biofilter with bee nest shaped has a large specific surface area so as to maximize the biodegradation process of pollutants by microorganisms. While bacteria consortium consisting of several types of bacteria have a greater capacity than a single bacterium in the degradation of pollutants. The research aims to determine the effect of starter volume and air flowrate to decrease the concentration of pollutants (BOD, COD, phenol and ammonia-free) in AF2B reactor batch using a bacterial consortium. The research was conducted in three phase which are the making of growth curve, acclimatization and biodegradation of pollutants in an AF2B reactor batch using a bacterial consortium. The experiment variables are the starter volume (85%, 75%, 65% in volume), and the air flowrate (2.5; 5; and 7.5 liter/min). Materials used are hospital waste water and bacterial consortium. The experiment begins with setting up the AF2B reactor containing biofilter with bee nest shaped and then filled it with a starter from the acclimatization process on a given volume and followed by supplies of air at a certain flowrate. Then hospital waste water and 5% of nutrients were added till reach 10 liters of total volume in reactor. Samples were taken every 30 minutes for up to 360 minutes of biodegradation process. The samples were then analyzed its pollutants concentration (BOD, COD, phenol and ammonia-free). The BOD concentration was analyzed using Winkler bottles method, COD using open reflux method, while phenol and ammonia-free using UV-Vis spectrophotometry method. The results showed that the starter volume and air flowrate affect the decrease of pollutants concentration till reach quality standard, which at starter volume of 85%, air flowrate of 7.5 liter/min and biodegradation time of 360 min can reduce the BOD, COD, phenol, and ammonia-free to 92%, 86%, 88% and 76% respectively.
RFLP Marker Variation of Cytocrome b Gene and Genetic Relationship among Batur, Merino and Local Sheep Breeds Prayitno, Prayitno; Hartati, T; Pratiwi, R; Artama, WT
ANIMAL PRODUCTION Vol 13, No 3 (2011): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.057 KB)

Abstract

RFLP analysis of mitochondrial DNA cytochrome b gene was conducted to determine the diversity, status and close genetic relationships in a population of Batur sheep with the Merino and local sheep breeds (Garut, Thin Tail and Fat Tail). The research used genomic DNA of 27 samples of Batur, 15 Merino, 17 Garut, 15 Thin Tails and 15 Fat Tails sheep. The PCR process used two types of 25 nucleotides primers. The PCR products were checked by using 2% agarose gel electrophoresis. The PCR DNA fragment was digested by using Hae III at 37 oC and incubated for 10 hours. Similarities and differences of cytochrome b gene RFLP bands between individual samples of one and across populations, genetic distance, and close genetic relationship, were identified. The PCR process of the cytochrome b gene metochondrial DNA of the 45 samples of sheep yielded 359 bp band types. The digestion (cutting) of the PCR products of mitochondrial DNA cytochrome b gene by using Hae III resulted in RFLP band profiles of 128 up to 231 bp polymorphisms of cytochrome b gene. Although the Hae III restriction enzyme recognized only one restriction site, however, between samples of Batur, Merino, Garut, Thin Tail, and Fat Tails, there were monomorphism and polymorphism Hae III loci.Key Words:  RFLP, cytochrome b gene, genetic markers, genetic similarity, Batur sheeAnimal Production 13(3):156-165 (2011) 
The Content of Lactic Acid and Lactose of Yoghurt Fermented with Different Number and Percentage Starter Bacteria Prayitno, Prayitno
ANIMAL PRODUCTION Vol 8, No 2 (2006): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.716 KB)

Abstract

The objective of the research was to study rate of lactose fermentation and production of lactic acid  by  Streptococus  thermophillus and Lactobacillus  bulgaricus. Yoghurt  was  made from  skim milk 10 percent  inoculated  with  Streptococus  thermophillus and  Lactobacillus  bulgaricus different number  and  percentage.  The  research  was conducted  in a completely  randomzed design factorial  pattern  3 x 4.  The  first  factor was  different (R) of  Strepcocus  thermophillus and Lactobacillus  bulgaricus 109 : 109 (1 : 1), 109 : 2x109 (1 : 2)  and 2x109 : 109 (2 : 1), and second factor was  percentage (S)  of starter, with  were  1, 2, 3  and  4 percent.  Each  treatment  has three replicated.  Variabels  measured  ware lactic acid  and  lactose content  of yoghurt after  12 hours  fermented.  Lactic acid content  was determined   by titration  and lactose  content by  spectrofotometry.  Result  showed that number  of Streptococus thermophillus and Lactobacillus  bulgaricus and percentage  of starter, including  ther  interaction, have  very hight  significant  (P<0,01) affect  on  lactic  acid and  lactose  content  of yoghurt.   For  resulted  the yoghurt  with  the best  quality (midle   of  lactic  acid  and  lower lactose content) recommended  to used  Streptococus  thermophillus and  Lactobacillus  bulgaricus 1 : 2 with  4 percent  starter  addition. (Animal Production 8(2): 131-136 (2006) Key Words  : Streptococus thermophillus, Lactobacillus bulgaricus, starter, yoghurt, lactic acid, lactose
Increasing of Energy Quality of Waste Product with Trichoderma and Aspergillus Suwandyastuti, SNO; Subarjo, B; Rimbawanto, EA; Prayitno, Prayitno; Irawan, I; Zubaidah, S
ANIMAL PRODUCTION Vol 1, No 1 (1999): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Biomasa of agricultural residues are potensial as ruminant feeds. However due it is low palatability, digestibility and nutritive value limited their use. In order to improve their use, treatment need to be applied. Biological treatment by using microba seems to be an alternative because of their capability with no pollution problems. The first experiment aims to select the microorganism which have a potensial to degrade the crude fiber, based the production of reduction sugar. The second experiment aims to improve the protein and amino acid on rice straw, cassava, waste, and rice husk, by inoculated the starter of Candida utilis and or Sacharomyces cerevise. The second experiment has been conducted on Animal Nutrition and Feed Laboratory, Faculty of Animal Husbandry UNSOED for eight month Fermentation trial has been done in semi solid media, by the method of Kjic (1964), in Batch System, Variables measure were: (1) reduction sugar, (2) cellulose, (3) protein, (4) amino acids, (5) cellulase activity, (6) essensial mineral and (7) energy. Based on the all variables measured that were conclused that the quality of  rice straw can be improved by mixed culture of  T, viride – S. cerevise, the rice husk by A. niger – C. utilis, T. viride – C. utilis and A. niger – S cerevise while for cassava waste by  A. niger – S. cerevise and A. niger – C. utilis  (Animal Production 1(1) : 10-16 (1999). Key Words: Waste Product, Energy, Microorganism
Determination of α-Amylase Enzyme Activity and Blood Glucose Level in Local Duck Prayitno, Prayitno; Ismoyowati, Ismoyowati; Farida, Ida
ANIMAL PRODUCTION Vol 5, No 1 (2003): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.367 KB)

Abstract

A α-amylase is included in hydrolase’s enzyme (E.C. 3.2.1.3), which catalyzed the breaking down of α-1,3-glycosidic bound on amylase chain and produced glucose as end product. In mammalian and poultry, α-amylase enzyme has a function as starch breaking down or changed glycogen to glucose. It was used as energy resource in the body. A α-amylase enzyme is protein that resulted in expression from one or several genes, so that has various characteristics among individual. To study the existence and the characteristic of α-amylase enzyme, therefore it has been conducted a research about the connection of α-amylase enzyme unit number  with glucose content in Tegal, Magelang and Mojosari duck blood (each of them consisted of 28 birds). This research used Completely Randomized Design (CRD) with seven replicates for each treatment. The result research showed that either the unit number of α-amylase enzyme activity or glucose content in these local breed of duck has a highly significant different (P<0.01). This result showed that genetic factor (breed of duck) has influenced either enzyme unit number or their catalytic activity on substrate, so the capability to form blood glucose inter breed of duck also different. It was suggested that their enzyme characteristics have strong connection with the sequence of amino acid as α-amylase enzyme protein composer, which was the result of gene expression. From the result, it was concluded that the unit number and catalytic activity of α-amylase enzyme and blood glucose content in the breed of local duck was affected by genetic factor (breed of duck). (Animal Production 5(1): 50-56 (2003) Key words: Enzyme, K-Amylase, Blood, Glucose, Duck
Effect of Specific Lactate Dehydrogenase Antigen from Sheep with Different Prolific Type on Hematology and Antibody Responses of Chicken Sumaryadi, MY; Prayitno, Prayitno; Manalu, W
ANIMAL PRODUCTION Vol 9, No 1 (2007): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Maternal serum of thirty nine ewes were used to study hematological and antibody responses of chicken injected with antigen protein of specific LDH obtained from sheep with different littersizes (prolific type). The prolific type of ewes was determined base on littersize (LS), which were low ( 1 ≤  LS ≤ 1.5), medium ( 1.5 < LS ≤ 2.0), and high (LS > 2.0). There were ten ewes carrying specific LDH protein. The number of animals of low, medium, and high prolific-type ewes was 3, 5, and 2 heads, respectively. The serum was used to produce antigen and injected to chicken to promote antibody production of anti LDH. The results of research showed that different prolific-type has no significant (P>0.05) effects on hematological and antibody titer of chicken.  However, hematological status of chicken injected with specific LDH antigen was significantly (P<0.05) higher than control. It can be concluded that production of specific protein anti LDH can be done in layer by injection of antigen from ewes. Product of specific protein anti LDH of prolific ewes still needs field test for its accuracy for selection of ewes with different prolific type. (Animal Production 9(1): 40-44 (2007) Key Words: hematology, antibody, LDH, ewes 
Co-Authors A. Muri Yusuf A.A. Ketut Agung Cahyawan W Abdul Jami ABDUL MANAF Afrillia, Ellysa Rahma Agnes C Davinchi, Agnes C Agus Basukesti, Agus Agustin, Faadhilah Agustina, Nabilah Fauziah Aji, Yuangga AL-FAIZI, M. Al-Kamel, Mujib Alrahman Amalia, Nabila Tasya Amir Syamsudin Anas Anas, Anas Anatasya, Anatasya Anif, Anif Annisa, Fitri Any Setyaningsih, Any Ardhana, Naufal Reky Ardiansyah, Septian Dicky Arief Rahmatulloh Arifiyanti, Nurul Arleiny, Arleiny Arrayyan, Ahmad Zaki Asalil Mustain Astuti, Fina Andika Frida B Subarjo, B Bambang Priyono Basuki , Bryliani, Fadya Haya Budi Murtiyasa Chairunnisa, Nabila Chen, Hsing-Chung Christiana Maria Herry Purwanti, Christiana Maria Herry Dattadewi Purwantini Dedi Andrianto Dewajani, Heny Diana Rachmawati Dina Sukma Dwi Ningsih, Dwi Dwi Wahini Nurhajati, Dwi Wahini EA Rimbawanto, EA Eka Putra, Herman Estu Eka Sapti Cahyaningrum Ekasari, Silvia Rahmi Emiliana Kasmudjiastuti, Emiliana Endro Kismolo, Endro Fadhilah, Alya Nur Fadhli, Alfi Fahriah, Sirli Fanani, Fanisia Salsyabila Faniansyah, Adias Faris Nofandi Febrianto, Firman Fikri, Dimas R. A. Fitriana Tjiptasari Fitriyani, Rizki Putri Fitriyanti, Dini Fuadah, Puteri Annisa Tsamrotul Gunawan, Lieptiono Hadi Musthofa, Hadi Hadi Saroso Hafni Lissa Nuri Hafsah, Diana S. Hafsah, Diana Sayyidah Halim, Amran Hanum, Muthia Hardiyanti, Widya Dwi Hardjono Hardjono, Hardjono Haren, Hassan Ishag Hassan Hariyanto, Budi Hariyanto, Budi Hariyanto Harun Harun Heppi Sasmita Herman Nirwana Herminiwati Herminiwati, Herminiwati Hesti Hesti Hidayatinnisa, Nurul Huda, Chaerul I Irawan, I Imanto, Frenki Iryouw, Veronika Ismoyowati Isra, Fauzi Iswara, Mochammad Agung Indra Jamaris Jamaris Jaya, Yahya Jaya, Yahya Johana A. Rombang, Johana A. Joko Pamungkas Karneli, Yenni Kartika, Vinda Setya Khalimatus Sa'diyah Kuntardjo, Samuel Beta Kuntarjo, Samuel Beta Kurnianingsih Kurnianingsih, Kurnianingsih Kusuma, Rieza Mahendra Lavindi, Eri Eli Luchis Rubianto3 Lusiani, Cucuk Evi M. Pancoko M. Y. Sumaryadi Maharani, Oktaviana Mahardhika, Dimas Kembara Mahben Jalil Mardiyono Mardiyono Maria Y.M.A. Sumakud Markamah, Markamah Marliyadi Pancoko Matondang, Anas Monandar Mita Prastika, Mita Mochamad Mufti Moejiono, Moejiono Moh Djaeni mufid mufid Muhammad Anif MUHAMMAD SHOLEH Muhibbin, Muhibbin Mukhlis, Syaiful Mukhlis, Syaiful Mukhlis Mulyani, Indah Muji Muthmainah Muthmainah Muthmainah Nariswana, Rucirasatti Nelva Rolina Netrawati, Netrawati Nono Carsono Nur Cholimah Nur Hayati Nur Khoirani, Alfina Nur Wachid Sahadi, Nur Wachid Nur Widiastuti Nurfarida Deliani Nuria, Aghna Nurrachma, Alifia Luzza Nurseno Bayu Aji, Nurseno Bayu Paniklan, Vastu Kayhan Sang Prasetya, Desta Enggar Dwi Prasetyo Kusumo, Mahendro Pratama, Sakca Garda Prayogo, Imam Profiyanti Hermien Suharti Puji Ediari Suryaningsih, Puji Ediari Putra, Karisma Putri, Eka Emiliana Putri, Priskila O. R Pratiwi, R R. Jaka Susila, R. Jaka Rahkman, Rizqi Aini Rahmadani, Riyan Rahmadhani, Nurindah Rahmat Hidayat Rahmawati, Hawa Yaumi Rahmiati Rahmiati Rani Raharjanti Rezmelia Sari Ridantami, Vemi Rina Sofiana, Rina Rina Wulandari Rizal Isnanto, Rizal Rohmaniah, Siti Romanda Annas Amrullah Rulianah , Sri S Zubaidah, S S.N.O. Suwandyastuti Safitri, Zulfa Samsu Wasito Sandra Santosa Santi Novani Santosa, Naufal Adli Santoso, Kuwat Saputri, Sisca Meidina Saputri, Sisca Meidina Sari, Siska Difita Sensih, Dinda Gusti siti zubaedah Sofyan karani, Sofyan Sri bandi, Sri Sri Budiasih, Sri Sri Rulianah, Sri Sri Sutyasmi, Sri Sri Wahyuni Sri Waskito, Sri Subagyo Subagyo Sudaryanti Sudaryanti Sugihartono Sugihartono Sulha, Balgis Suliestiyah Wiryodiningrat Sulistio, Any Suprapto Suprapto Susilo , Sugeng Hadi Sutama SUTRISNO Sutrisno, Sutrisno Syafitri, SIti Balqis Syarif Maulidin T Hartati, T T Hartatik, T Thomas Tukirin, Thomas Titik Purwati Widowati, Titik Purwati Tri Kurniati, Meutea Tri Raharjo Yudantoro Triyono, Liliek Utomo, Dian Purbo W Manalu Wahono Wahono Wahyu Sulistiyo Wakib Kurniawan Wicaksana, Darma Widyastuti, Ervina Dewi Wijayanti Putri, Aprillia Wiktasari Wiktasari, Wiktasari WT Artama, WT Yanti, Nurma Yanwari, M. Irwan Yanwari, Muhammad Irwan Yeni Karneli Yudantoro, Tri Yuliani Indrianingsih Yunia, Yessy Prisca Z. Mawardi Effendi Z., Dini Arina Fasa Zahroh, Nur Alfiatuz Zakiyyah, Dini A. F.