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Robusta Coffe-Derived Silver Nanoparticles as a Natural Photosensitizer for Photodynamic Inactivation of Bacterial Biofilms Amruloh, Yazid Muhammad; Astuti, Suryani Dyah; Arifianto, Deny; Nurdin, Dezy Zahrotul Istiqomah; Zaidan, Andi Hamim; Widiyanti, Prihartini; Yaqubi, Ahmad Khalil; Winarno; Abd Razak, Nasrul Anuar
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.74709

Abstract

Bacterial biofilm formation represents a major challenge in clinical settings, as it contributes to persistent infections and resistance to conventional antimicrobial treatments. This study investigates the effectiveness of blue laser therapy combined with AgNPs-CR in inhibiting biofilm formation by Escherichia coli and Staphylococcus aureus, common infection agents, in a four-group sample set. Silver nanoparticles, known for their antibacterial, antifungal, and antiviral properties, are widely used in biomedical devices. First, the control group (T0) included samples without laser irradiation. Second, the Escherichia coli and Staphylococcus aureus groups (A1 and A2) were irradiated with a 405 nm diode laser at various exposure times and silver nanoparticle concentrations. Third, the E. coli and S. aureus groups (A3 and A4) were also irradiated with the 405 nm diode laser under varying durations and concentrations of silver nanoparticles. In groups A2 and A4, the bacteria were treated with the photosensitizer AgNPs-CR, incubated for 30 minutes, and then irradiated for 90, 120, 150, and 180 seconds. The results were analyzed using a two-way factorial ANOVA and a Post-hoc Tukey test, with a significance level of p < 0.05. Photodynamic therapy (PDT) significantly inhibited bacterial biofilms in samples with irradiation, with the most significant reduction observed at a concentration of 2 mM and 180 seconds of irradiation, compared to samples without irradiation. Staphylococcus aureus was the most susceptible to AgNPs, showing a high biofilm inhibition percentage of 94.82% at 180 seconds of irradiation with a 2 mM concentration. Fluorescence microscope analysis revealed the presence of bacterial DNA corresponding to 180 colonies.
Penerapan Metode Pembelajaran Debat Aktif untuk Membangun Sikap Demokratis Siswa pada Mata Pelajaran Pendidikan Pancasila Wijayanto, Luqman; Al Rasyid, Machmud; Winarno
Civics Education and Social Science Journal (CESSJ) Vol. 7 No. 2 (2025)
Publisher : Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/cessj.v7i2.7279

Abstract

Penelitian Tindakan Kelas (PTK) ini meneliti penerapan metode debat aktif untuk membangun sikap demokratis siswa dalam mata pelajaran Pendidikan Pancasila di SMAN 2 Boyolali. Penelitian dua siklus ini melibatkan siswa kelas XI-7 dengan teknik pengumpulan data wawancara, observasi, dan studi dokumen, yang dianalisis secara kualitatif dan kuantitatif. Hasil penelitian membuktikan keefektifan metode debat aktif. Rata-rata sikap demokratis siswa meningkat signifikan dari 50% pada pra-siklus menjadi 62,5% pada siklus pertama lalu 87,5% pada siklus 2, dengan indikator toleransi terhadap perbedaan mencapai 100%. Partisipasi siswa juga naik dari 68,75% menjadi 93,75%. Peningkatan ini didukung oleh pendekatan terstruktur, pengalaman belajar nyata, dan peran guru sebagai fasilitator. Tantangan seperti ketimpangan partisipasi dan manajemen waktu berhasil diatasi melalui pelatihan debat, pembagian peran, dan evaluasi berbasis umpan balik. Kesimpulannya, debat aktif efektif membentuk sikap demokratis ketika didukung perencanaan matang, topik kontekstual, dan lingkungan inklusif. Implikasinya, guru disarankan mengadopsi metode interaktif ini untuk menciptakan pembelajaran yang partisipatif. Penelitian lanjutan dapat mengembangkan modul debat berbasis isu lokal agar pendidikan demokrasi tidak hanya teoritis tetapi juga melatih keterampilan sosial siswa sebagai warga negara. ReferensiAbdussamad, Z. (2021). Metode penelitian kualitatif. Syakir Media Press. Arends, R. (2012). Learning to teach (9th ed.). McGraw-Hill. Arends, R. (2015). Learning to teach (10th ed.). McGraw-Hill. Bandura, A. (1986). Social foundations of thought and action: A social cognitive theory. Prentice-Hall. Bernadetta, S., dkk. (2021). Action research in education: Theory and practice. Routledge. Brookfield, S. D. (2017). Becoming a critically reflective teacher (2nd ed.). Jossey-Bass. Dewey, J. (1916). Democracy and education: An introduction to the philosophy of education. Macmillan. Hattie, J. (2009). *Visible learning: A synthesis of over 800 meta-analyses relating to achievement*. Routledge. Johnson, D. W., & Johnson, R. T. (1999). Learning together and alone: Cooperative, competitive, and individualistic learning (5th ed.). Allyn & Bacon. Johnson, D. W., & Johnson, R. T. (2014). Cooperative learning in the classroom. Interaction Book Company.Kementerian Pendidikan dan Kebudayaan. (2022). Panduan pembelajaran pendidikan Pancasila fase F. Kemendikbud.Kementerian Pendidikan, Kebudayaan, Riset, dan Teknologi. (2021). Pedoman implementasi kurikulum merdeka. Kemendikbudristek. Lickona, T. (1991). Educating for character: How our schools can teach respect and responsibility. Bantam. Lie, A., dkk. (2020). Debate pedagogy in Asian contexts. Springer. Majid, A., & Rochman, C. (2020). Pembelajaran interaktif: Teori dan praktik. Remaja Rosdakarya. Miles, M. B., & Huberman, A. M. (2014). Qualitative data analysis: A methods sourcebook (3rd ed.). SAGE. Murdiyana, E. (2020). Validitas data penelitian kualitatif. Universitas Brawijaya Press. Nurdin, E. S. (2022). Model debat aktif dalam pembelajaran. Prenadamedia Group. Nussbaum, M. C. (2010). Not for profit: Why democracy needs the humanities. Princeton University Press. Patton, M. Q. (2015). Qualitative research & evaluation methods (4th ed.). SAGE. Putnam, R. D. (2000). Bowling alone: The collapse and revival of American community. Simon & Schuster. Rosenberg, M. J. (2021). E-learning: Strategies for delivering knowledge in the digital age (2nd ed.). Pfeiffer. Sapriya. (2012). Pendidikan kewarganegaraan: Konsep dan pembelajaran. Rosda. Sapriya. (2019). Pendidikan nilai dalam pembelajaran PKn. Remaja Rosdakarya. Slavin, R. E. (2014). Cooperative learning: Theory, research, and practice (3rd ed.). Pearson. Suharsimi, A. (2006). Prosedur penelitian: Suatu pendekatan praktik (Edisi Revisi). Rineka Cipta. Tomlinson, C. A. (2017). How to differentiate instruction in academically diverse classrooms (3rd ed.). ASCD. Trilling, B., & Fadel, C. (2009). 21st century skills: Learning for life in our times. Jossey-Bass. Vygotsky, L. S. (1978). Mind in society: The development of higher psychological processes. Harvard University Press. Zamroni. (2017). Dinamika pendidikan karakter. UNY Press.
Penerapan metode Silver Meal untuk optimalisasi persediaan bahan baku obat nyamuk bakar pada PT. FNM Sukma Pamungkas; Winarno
JENIUS : Jurnal Terapan Teknik Industri Vol 7 No 1 (2026): JENIUS: Jurnal Terapan Teknik Industri
Publisher : LPPMPK - Universitas Muhammadiyah Cileungsi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37373/jenius.v7i1.1950

Abstract

Permintaan produk obat nyamuk bakar di Indonesia mengalami peningkatan signifikan seiring tingginya kesadaran masyarakat akan bahaya penyakit yang ditularkan nyamuk. PT FNM sebagai produsen obat nyamuk menghadapi tantangan dalam pengendalian persediaan bahan baku Tepung Batok akibat fluktuasi permintaan bulanan yang menyebabkan risiko overstock maupun out of stock. Penelitian ini bertujuan untuk menerapkan metode Silver Meal dalam mengoptimalkan pengendalian persediaan bahan baku dan menghitung total biaya yang dikeluarkan perusahaan. Pendekatan yang digunakan adalah metode kuantitatif dengan mengolah data historis pemakaian bahan baku tahun 2024. Data diperoleh melalui observasi, wawancara, dan dokumentasi dari bagian PPIC perusahaan. Metode Silver Meal digunakan untuk menentukan ukuran lot pemesanan yang optimal dan frekuensi pemesanan yang efisien dengan mempertimbangkan biaya pemesanan dan penyimpanan. Hasil pengolahan data menunjukkan bahwa penerapan metode Silver Meal menghasilkan efisiensi yang signifikan, dengan total biaya persediaan sebesar Rp336.523.489 selama satu tahun. Metode ini terbukti dapat menurunkan total biaya dibandingkan dengan sistem pemesanan sebelumnya yang bersifat intuitif. Kesimpulannya, penerapan metode Silver Meal dapat meningkatkan efisiensi pengelolaan persediaan bahan baku di PT FNM dan dapat dijadikan sebagai strategi pengendalian persediaan yang lebih akurat dan ekonomis dalam menghadapi permintaan yang fluktuatif.
Development of Interactive Learning Media Using Genially Based on Game-Based Learning Kuncoro, Bhakti Dwi; Wijianto; Winarno
Research Horizon Vol. 5 No. 6 (2025): Research Horizon - December 2025
Publisher : LifeSciFi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54518/rh.5.6.2025.929

Abstract

This study aims to develop interactive learning media using the Genially platform with a Game-Based Learning approach and to examine its feasibility and effectiveness in improving learning outcomes. This research employed a research and development method based on the Sukmadinata model, which consists of three stages: preliminary study, product development, and product testing. Data were collected through observation, interviews, questionnaires, surveys, and learning achievement tests. The research subjects included material experts and media experts for validation, 12 students of class VIII B in the limited trial, and 32 students of class VIII A in the field trial. Media feasibility was determined based on expert validation and students’ responses. The results indicate that the Genially-based learning media was rated “Very Feasible” by material experts with a score of 93 and by media experts with a score of 85. Students’ responses showed a mean score of 85.5, categorized as “Very Good.” The effectiveness analysis using N-Gain based on pretest and posttest scores yielded a value of 70.05%, indicating a “Moderately Effective” level. These findings confirm that Genially-based game-based learning media can enhance students’ engagement and learning outcomes.
Co-Authors A. K. Islami A. M. Fuah A. M. Fuah, A. M. A. Sunyoto Abd Razak, Nasrul Anuar Abdus Salam, Abdus Abror, Ghulam Muhammad Achmad Afandi, Achmad ADI BASHORY, KHARISMA Adiputra, Agung Agastya, Yoga Agusti Mardikaningsih AGUSTIE, TEO Agustin, Eva Inaiyah Ahmad Bashori Ahmad Junaidi Alfandi, Aril Alma, Ahmad Faizin Alvin Indra Pratama Amruloh, Yazid Muhammad Anak Agung Ngurah Perwira Redi Andi Hamim Zaidan Andrea Salsalova Annida Lathifa Riandy Putri Anthoni Arif P Arifianto, Deny Aril Afandi Arum Filsafat Ary Artanty Ashari Husen Ashari, Minanti Mayda Audina P Alhabsyi, Gina B. K. Sheehan Berlianisa, Kumala Bramastia C. D. Darmawan Chairul Andrian D. A. Astuti Dandung Wahyu Amrulloh Deddy Chrismianto Deden Ramdani DELLITA, WINDA Demon, Fransiskus Dominikus Deni Zein Tarsidi Destiani, Reza Dian Neli Pratiwi Dirga Candra Styawan Djono DWI DAMAYANTI, ASTI Dwi Setiawati Radjak E. A. Syahrin Edeh Dike Mariske Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Elsyea Adia Tunggadewi Endang Siswati Endrise Septina Rawanoko Febriansyah, Muhammad Ferdy Rehan Pane Gandhi Adidaya H. Nuraini Handoko, Hendri Hari Pamungkas Hendik Murdiyanto Hery Sawiji Hidayah, Ferro Hieronymus Purwanta Hilhamsyah I Made Mariasa I. Nabilah Ika Mariyani Iknasius Lagu Imam Rofiki Indah Kurniawati Isnaini, Illahika Qurotul JULI SAPTA RAHITA, DWI Juliani Pudjowati Kartika Chrysti Suryandari Khusnul Ain Khusnul Ain Kuncoro, Bhakti Dwi Lantapi, Adriansyah Wuriadin Lantapi Leo Agung Sutimin Lestari, Septi Wahyu Luthfiyah, Sari Machmud Al Rasyid, Machmud Maharani, Annisa Lidya Melandhany, Mareta Minanti Mayda Ashari Mintasih Indriayu Mochammad Balafif Mohammad Muchtarom Mudjia Rahardjo Muhammad Febriansyah Muhammad Hendri Nuryadi, Muhammad Hendri Muhammad Hufron Muhammad Kyodan Khalidzky Muhammad Nazmi Saputra Muhlisin Murdiyanto, Hendik Mushofi, Yuskhil N. C. Hartono Natanael Suranta Natasya Thesalonika Nobuo Funabiki, Nobuo Nurdin, Dezy Zahrotul Istiqomah Nursim Okto Risdianto M Pakombong, Vhalentio Pamorti, Ongke Ageng Paulus Rah Adi Pawitra Permatasari, Nindy Permatasari, Perwira Annissa Dyah Pipit Widiatmaka, Pipit Prastyo, Bayu Pratiwi, Dian Neli Prihantoro, Eko Prihartini Widiyanti Priyambodo Pujiyanto Purnama Adi, Fadhil R Yogi Nugraha Raden Mohamad Herdian Bhakti Rahma, Zuyyina Rahman, Safri Ramadhani, Nadia Nur Ramli Rawanoko, Endrise Septine Reno Ismanto Rohman, Muchammad Nurur Salamet Salatnaya, H. Salsalova, Andrea Sandra, Shifa Septi Wahyu Lestari Septilia Regita Cahyani Shifa Sandra Siswanto Soegianto Soelistiono Sudari Sudarsono Sugeng Sholehuddin, Sugeng Suhada Suhada, Suhada Suhariningsih Sukma Pamungkas Sulaeman Suparman Suprobo, Yok Suryani Dyah Astuti Susi Tri Wahyuni Susilo, Yunus Sutimin , Leo Agung Sutimin, Leo Agung Syahrizal Syahrom, Ardiyansyah Syaiful Alam SYAIFUL RAMADHAN, AHMAD Syarifullah Bundang Syuhadah, Nurul Aisyah T. Suryati Thesalonika, Natasya tifani Tri Murwaningsih Triana Rejekiningsih dkk Triuli Novianti Triuli Novianti, Triuli Triyanto V. A. Mendrofa VALDIANSYAH, RAKA WAHYU PRASETYO, YOGA Wahyudi, Edi Wati, Evita Ratna Widodo, W. D. Widya Noventari, Widya Wijayanto, Luqman Wijianto Wijianto Wildan Fatchan Maulidin Y. C. Endrawati Yaqubi, Ahmad Khalil Yoga Duta Bartila Yonatan Yuliana Andriyani Yulianto, Fazri Yunardi, Riky Tri Yunita Maysyaroh Yuskhil Mushofi Zain, Nuril Jannah