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Polymorphism of Plasmodium Falciparum Dihydrofolate Reductase and Dihydropteroate Synthase Genes among Pregnant Women with Falciparum Malaria in Banjar District, South Kalimantan Province, Indonesia Fitriah, Fitriah; Sulistyawati, Sriwijayanti; Riyanto, Sugeng; Budiono, Budiono; Basuki, Sukmawati; Dachlan, Yoes P; Uemura, Haruki
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Pregnant women are highly vulnerable to malaria infection in its endemic areas, particularly infection by Plasmodium falciparum that can cause premature, low birth weight, severe anemia in pregnant women, and death. Sulfadoxine-pyrimethamine (SP) for Intermittent Preventive Treatment for pregnant (IPTp) is used for malaria control in pregnancy recommended by the World Health Organization that has already been implemented in Africa. The P. falciparum resistance to SP has been reported in several malarial endemic areas, and mutations in the genes of Plasmodium falciparum Dihydrofolate Reductase (Pfdhfr) and Dihydropteroate Synthase (Pfdhps) are shown to be associated with parasite resistance to SP treatment. Genetic analysis of Pfdhfr and Pfdhps genes in pregnant women infected with P. falciparum has not yet been examined in Indonesia. The cross-sectional study was conducted at two subdistricts, Sungai Pinang and Peramasan, in Banjar district of South Kalimantan Province, where 127 pregnant women were recruited from 2008 to April 2010. Two important mutations in Pfdhfr gene (amino acid positions at N51 and S108) and three in Pfdhps gene (A437, K540 and A581) were analyzed by nested PCR-RFLP method. All of the seven pregnant women samples infected with P. falciparum presented PfDHFR 108N and PfDHPS 437G mutations. One of the samples had the additional mutation at PfDHPS 540, in which Lys is substituted by Glu. These results suggested that P. falciparum might present only some resistance to SP at Sungai Pinang and Peramasan subdistricts, Banjar District, South Kalimantan province, Indonesia. Although there were limited number of samples, this study showed only few mutations of Pfdhfr and Pfdhps genes in P. falciparum at Banjar district, South Kalimantan Province, that suggests SP might be effective for IPTp in this area. Thus, further analysis of the other mutation sites in Pfdhfr and Pfdhps genes and in vivo efficacy study of SP with more sufficient sample numbers will be necessary to confirm this preliminarily result.Keywords: PfDHFR and PfDHPS genes, Pregnant women, Falciparum malaria
Polymorphism of Plasmodium Falciparum Dihydrofolate Reductase and Dihydropteroate Synthase Genes among Pregnant Women with Falciparum Malaria in Banjar District, South Kalimantan Province, Indonesia Fitriah Fitriah; Sriwijayanti Sulistyawati; Sugeng Riyanto; Budiono Budiono; Sukmawati Basuki; Yoes P Dachlan; Haruki Uemura
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pregnant women are highly vulnerable to malaria infection in its endemic areas, particularly infection by Plasmodium falciparum that can cause premature, low birth weight, severe anemia in pregnant women, and death. Sulfadoxine-pyrimethamine (SP) for Intermittent Preventive Treatment for pregnant (IPTp) is used for malaria control in pregnancy recommended by the World Health Organization that has already been implemented in Africa. The P. falciparum resistance to SP has been reported in several malarial endemic areas, and mutations in the genes of Plasmodium falciparum Dihydrofolate Reductase (Pfdhfr) and Dihydropteroate Synthase (Pfdhps) are shown to be associated with parasite resistance to SP treatment. Genetic analysis of Pfdhfr and Pfdhps genes in pregnant women infected with P. falciparum has not yet been examined in Indonesia. The cross-sectional study was conducted at two subdistricts, Sungai Pinang and Peramasan, in Banjar district of South Kalimantan Province, where 127 pregnant women were recruited from 2008 to April 2010. Two important mutations in Pfdhfr gene (amino acid positions at N51 and S108) and three in Pfdhps gene (A437, K540 and A581) were analyzed by nested PCR-RFLP method. All of the seven pregnant women samples infected with P. falciparum presented PfDHFR 108N and PfDHPS 437G mutations. One of the samples had the additional mutation at PfDHPS 540, in which Lys is substituted by Glu. These results suggested that P. falciparum might present only some resistance to SP at Sungai Pinang and Peramasan subdistricts, Banjar District, South Kalimantan province, Indonesia. Although there were limited number of samples, this study showed only few mutations of Pfdhfr and Pfdhps genes in P. falciparum at Banjar district, South Kalimantan Province, that suggests SP might be effective for IPTp in this area. Thus, further analysis of the other mutation sites in Pfdhfr and Pfdhps genes and in vivo efficacy study of SP with more sufficient sample numbers will be necessary to confirm this preliminarily result.Keywords: PfDHFR and PfDHPS genes, Pregnant women, Falciparum malaria
Co-Authors Abd. Syakur Abdul Aziz Abdul Chalid Ahmad Afif Nurul Hidayati, Afif Nurul Ageng Triyono Agus Frianto Agus Tinus Ah Yusuf, Ah Aindrianingsih, Aindrianingsih Aini, Wadzifatu Qurrotu Ainistikmalia, Nurin Akhmad Akhmad Alif Tiwi Estriyanti Alpha Fardah Athiyyah Amalia, Firdha Aminuyati Anang Kistyanto Andre Dwijanto Witjaksono Anisya Fitri Aprilia Wulan Arhamuddin Ali, Arhamuddin Arief, Budi Arif Prasetyo Wibowo Arini Julia Wardani Ariyanti , Jeny Mellysa Armida Salsiah Alisjahbana Askandar Tjokroprawiro Asri Wijayanti Astindari Astindari, Astindari Astuti, Ary Dwi AYU WULANDARI Ayuwangi, Bunga Badu’l Mat Saleh Bagus Haryo Kusumaputra, Bagus Haryo Baksono Winardi Bayu Kharisma Budi Utomo Candra Perbawati Candra Rahma Wijaya Putra Cindy Fransisca, Cindy Cita Rosita Sigit Prakoeswa Dachlan, Yoes P Damayanti Damayanti Darmadi Darmadi Darmadi Darmawati, Ratna Puspa Dedi Junaedi, Dedi Dewi Ayu Lestari Dewi, Gita Kusuma Dewie Trie Wijayati Diah Mira Indramaya Diyah Fatmasari Diyah Putri Permata Djoko Sutikno Dominicus Husada Dwi Murtiastutik Dwi Puji Rahayu Rahayu dwiarko nugrohoseno Dwiarko Nugrohoseno Dwijanto Witjaksono , Andre Dwiprabowo, Risky edy suyanto Effendi, Nuri EKO WAHYUDI Endang Susilaningsih ERI PERDANA USHAN Evy Ervianti Failasufa, Hayyu Faizah Yunianti Farid Farid, Farid Fatwarini, Aulia Faujiah, Evi Febrilio, Erlang Prawidha Ferry Hadiyanto Fitriah Fitriah Fitriah Fitriah Giovanni, Giovanni Gunadi Ranu, I Gusti Made Reza Hadi, Hafid Kholidi Hadi, Khusnul Hafid Kholidi Hadi Hanan, Abd. Handoyo Handoyo Hari Windu Asrini Harningtyas, Citra Dwi Hartati Hartati Hartoyo Hartoyo Haruki Uemura Hasibuan, Muhammad Arif Haspada, Deny Herawati, Tutik I Gusti Made Reza Gunadi Ranuh Ika Rahmania Indrian Supheni Iriani, Wahyuni Irianto, Singgih Irmadita Citrashanty, Irmadita Islamiyah, Wardah Rahmatul Januardi, Dicky Jayanti, Lira Wiet Jun Surjanti Khoirunnisa, Nabila Farah Kisyani, Kisyani Kusumaningum, Trias Madanika Laila Kurnia Sari, Laila Kurnia Lestari, Arfena Deah Liesnaningsih Liesnaningsih Linda Astari, Linda Lirawati, Lirawati Lucia Retnowati M Y. Probohoesodo M. Yulianto Listiawan Margono, Hendy Muagiri Maylita Sari Maylita Sari, Maylita Medhi Denisa Alinda, Medhi Denisa Menul Ayu Umborowati, Menul Ayu Miftakhul Jannah, Arinda Misbah, St. Rachmi Mochammad Meddy Danial Mudzakir, Abdul Rachman MUHAMMAD ALI Muhammad Faizi Mukti Ali, Mochammad Muryati Muryati Mustayah ., Mustayah Mustayah Mustayah Nabil Nabil, Nabil Nabilla, Maulidya Nartini, Nartini Nasharuddin Mas Ngestreini, Rahmadani Nadya Nihayati, Hanik Endang Nikie Astorina Yunita Dewanti Nisa, Ratih Rahmania Khoirun Nurbani Yusuf Nurfantri, Nurfantri Nurhasanah, Neli Nurimani, Nurimani Nurjazuli Nurjazuli NURUL HIDAYAH Nurul Pujiastuti Octavia, Dini Hariani Pasulu, Soraya Salle Pertami, Sumirah Budi Pramitha, Riezky Januar Pujiastuti, Nurul - Purnagunawan, Raden Muhamad Putri K. D., Putri K. D. Rachman Iswanto Rahayu, Dian Yuniar Syanti Rahmadewi Rahmadewi Ramdhany, Deny M. Renaldy, Raden Bagus Yanuar Ristya Sylva Imannia Rohmat Taufiq Roihan, lham Mashdar Rose Fitria Lutfiana Rosidawati Wiradirja, Imas Rossyanti, Lynda Rozaq, Khoirur Rudi Hamarno Rusliana, Nanang Salma, Zukhaila Sanusi Sanusi Saranani, Muhaimin Saraswati, Rina Sarjana, IWayan Sasmita, Agus Shalima, Irsyadi Shodiq, Samsul Sidarti Soehita Siswanto Darmadi Sita Hendrakusuma Samsu Siti Fatimah Soenaryo SITI KHOLIFAH Siti Wahyu Wulandari Slamet Wahyudi Sriwijayanti Sulistyawati Sudarman Sudarman Sugeng Riyanto Sugeng Riyanto Sugeng Sutiarso Sugiyanto - Sukmawati Basuki SULISTIYANI SULISTIYANI Supono Supono Suprianto, Herry Suryo Sardjono, Herning Suwandi Suwandi Suwarsono Suwarsono Syarifuddin Dahlan Tengku Riza Zarzani N Tony Seno Aji Tri Cahyo Sepdianto Tri Haryanto, Aris Trisniartami Setyaningrum Uemura, Haruki Umu Nur Latifah Vide Bahtera Dinastiti Vinzy Yulina Wati, Lira Wawan Hermawan Wicaksono, Galih Wasis Widiana, Putri Widyantari, Septiana Wina Aprilia Purnamasari Wiranto, Puji Wolayan, Grahana Ade Candra Yoes P Dachlan Yuandika, Nuke Yusniar Hanani Yuyun Isbanah Zahra, Farah Az Zamzam, Zamzam