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Coffea canephora Bean Extract Induces NIH3T3 Cell Migration Yessy Ariesanti; Ferry Sandra; Bianda Claresta; Livia Alvita
The Indonesian Biomedical Journal Vol 13, No 2 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i2.1522

Abstract

BACKGROUND: Wound healing is an essential biological process that consists of sequential steps aimed at restoring the architecture and function of damaged cells and tissues. There are empirical evidences of using pure coffee bean powder as an alternative medicine in treating various types of wounds. However, there is limited data on coffee-induced wound healing, especially migration of cells. Therefore, current study was conducted to investigate the role of coffee extract in cell migration, especially fibroblast which is important for wound healing.METHODS: Coffea canephora beans were prepared, extracted and added in the NIH3T3 cell culture in final concentration of 2.5% and 5%. Then cytotoxicity test was performed using Na,30-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzenesulfonic acid hydrate (XTT) assay. Meanwhile, cell migration test was performed with scratch assay. All results were statistically analyzed.RESULTS: The 2.5% or 5% Coffea canephora beans extract (CCBE)-treated NIH3T3 cell numbers were almost similar with the numbers of NIH3T3 cells in starvation medium merely. Meanwhile, 2.5% and 5% CCBE showed significant decrease of the widths of scratched areas compared to starvation medium merely (ANOVA with LSD Post-hoc, p=0.000). After 24 h and 48 h, the average widths of 2.5% and 5% CCBE-treated scratched areas were 235.68±22.79, 50.36±5.29, 229.95±23.01, 27.68±2.83, respectively.CONCLUSION: Since both 2.5% and 5% CCBE are potential in inducing migration of fibroblast (NIH3T3 cell) and do not induce cytotoxicity, the CCBE could be potential as an agent for wound healing.KEYWORDS: coffee, Coffea canephora, NIH3T3, migration, cytotoxicity
Survivin and Telomerase as Radiotherapeutic Response Predictors of Subjects with Stage IIIB Cervical Squamous Cell Carcinoma Fitriyadi Kusuma; Andrijono Andrijono; Ani Retno Prijanti; Laila Nuranna; Sri Mutya Sekarutami; Bambang Sutrisna; Ferry Sandra
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.813

Abstract

BACKGROUND: Cervical cancer is one of the most prevalent cancers in women. Even with similar clinicopathologic features, radiotherapy outcomes are still vary among patients. This research was conducted to measure radiotherapy responses on cervical cancer patients by using Survivin, Telomerase and Cytochrome C.METHODS: Subjects who matched the criteria were selected and requested to fill questionnaires. Subjects were then evaluated with magnetic resonance imaging (MRI) pre- and post-3D conformal radiotherapy. Histopathological study was conducted using resected tumors to determine the differentiation type. Enzyme-linked Immunosorbent Assays for detection of Survivin, Telomerase and Cytochrome C was performed using the resected tumors as well.RESULTS: There were 76 patients in this study. Mean ofage was 50 and diameter of tumor size was 5.35 cm. Mean levels of Survivin, Telomerase and Cytochrome C were 632.82 pg/mL, 5.59 pg/mL and 204.75 ng/mL, respectively. There were significant correlations between radiotherapy response and Survivin (p=0.041) or Telomerase (p=0.022). Subjects with lower Survivin level (<932 pg/mL) had higher 1-year survival rate (63%) than subjects with higher Survivin level (50%). Similar results were obtained for subjects with lower Telomerase level (<5.75 pg/mL), who had higher 1-year survival rate (60%) than subjects with higher Telomerase level (43%).CONCLUSION: Since radiotherapy response is significantly correlated with Survivin and Telomerase levels and subjects with lower Survivin or Telomerase level have higher 1-year survival rate, it can be suggested that Survivin and Telomerase could be potential predictors of radiotherapeutic response for subjects with stage IIIB cervical squamous cell carcinoma.KEYWORDS: cervical cancer, radiotherapy Survivin, Telomerase, Cytochrome C
Caffeic Acid Inhibits NFkappaB Activation of Osteoclastogenesis Signaling Pathway Ferry Sandra; Toshio Kukita; Quan Yong Tang; Tadahiko Iijima
The Indonesian Biomedical Journal Vol 3, No 3 (2011)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v3i3.153

Abstract

BACKGROUND: Caffeic acid (3,4-dihydroxycinnamic acids) is involved in various green plants. Based on our previous report, a major component of sweet potato extracts, possibly caffeic acid, was shown as a promising inhibitor of osteoclastogenesis. However, the effect of caffeic acid in inhibiting osteoclastogenesis needs to be confirmed. The underlying mechanism needs to be disclosed as well.METHODS: Caffeic acid in various concentrations was added to in vitro osteoclastogenesis of receptor activator nuclear factor kB ligand (RANKL)-tumor necrosis factor alpha (TNF-α)-macrophage colony stimulating factor (M-CSF)-induced bone marrow-derived monocyte/macrophage precursor cells (BMMs) and RANKL-TNF-α-induced RAW264 cells D-Clone (RAW-D cells). Tartrate resistant acid phosphatase (TRAP) staining was performed and TRAP-positive polynucleated cells (PNCs) were counted. For apoptosis analysis, caffeic acid-treated BMMs, RAW-D cells and osteoclast-like PNCs were subjected to Sub-G1 Apoptosis and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays. To measure NFkB activity, RAW-D cells were transfected with pNFkB-TA-Luc and subjected to Dual Luciferase Reporter Assay System.RESULTS: Caffeic acid inhibited osteoclastogenesis of RANKL-TNF-α-M-CSF-induced BMMs as well as RANKL-TNF-α-induced RAW-D cells in a dose dependent manner. Caffeic acid did not induce apoptosis in BMMs, RAW-D cells and osteoclast-like PNCs. RANKL-TNF-α-induced NFkB activity in RAW-D was diminished by caffeic acid in a dose dependent manner. Significant NFkB activity inhibtion was observed starting from 1 µg/mL caffeic acid. CONCLUSIONS: Caffeic acid could be a potent osteoclastogenesis inhibitor through inhibition of NFkB activity. Our present study should be further followed up to disclose caffeic acid's possible overlying signaling pathways in inhibiting osteoclastogenesis.KEYWORDS: caffeic acid, osteoclastogenesis, NFkB, RANKL, TNF-α
Cytotoxicity of Alpinia galanga Rhizome Crude Extract on NIH-3T3 Cells Ferry Sandra; Janti Sudiono; Pretty Trisfilha; Deviyanti Pratiwi
The Indonesian Biomedical Journal Vol 9, No 1 (2017)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v9i1.212

Abstract

BACKGROUND: Alpinia galanga (A. galanga) was reported as a potential medicinal source due to its wide effect. A. galanga rhizome crude extract (ARCE) was reported to have high cytotoxic effect in cancer cells, but low in normal cells. However half maximal inhibitory concentration (IC50) of ARCE is not clearly known yet. Hence, current study was conducted to investigate the IC50 of ARCE in normal standard fibroblast cell line, NIH-3T3 cells.METHODS: Rhizomes of A. galanga were collected, peeled, dried, milled and weighed. Extraction was performed using maceration method, then filtered and evaporated. ARCE with various concentrations were applied in NIH-3T3 cells for 24 or 48 hours. Cells were documented and counted with 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) assay.RESULTS: Five hundreds grams of simplicia were macerated with ethanol and evaporated, 1 mg/mL crude extract with total volume of 114 mL was obtained. By addition of ARCE in NIH-3T3 cell culture, number of NIH-3T3 cells were shown less when treated with higher concentration of ARCE. Cell numbers of 0, 3.125, 6.25, 12.5, 25 and 50% ARCE treatment for 24 hours are 11,531, 11,352, 10,920, 10,365, 9,471, 8,360, respectively, meanwhile for 48 hours are 13,219, 12,686, 12,278, 11,390, 10,279, 8,390, respectively.CONCLUSION: IC50 of ARCE in 24 hours treatment was 620.5 mg/mL, while in 48 hours treatment was 666.6 mg/mL. Hence, ARCE is suggested to have low cytotoxic effect in NIH-3T3 cells.KEYWORDS: Alpinia galanga, ginger, extract, cytotoxic, MTT, NIH-3T3 
Caffeic Acid Inhibits RANKL and TNF-α-induced Phosphorylation of p38 Mitogen-activated Protein Kinase in RAW-D Cells Ferry Sandra; Ketherin Ketherin
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.437

Abstract

BACKGROUND: Caffeic acid inhibits osteoclastogenesis by downregulating expression of Cathepsin K and Nuclear Factor of Activated T cells (NFATc)1, as well as inhibiting activity of Nuclear Factor kB (NFkB). Meanwhile TNF Receptor-associated Factor (TRAF)6 was not influenced by caffeic acid. In order to investigate further caffeic acid's mechanism in inhibiting osteoclastogenesis, regulation of caffeic acid on p38 Mitogen-activated Protein Kinase (MAPK) was investigated.METHODS: RAW-D cells were pretreated with/without caffeic acid and treated with/without 20 ng/mL RANKL and 1 ng/mL TNFα for 0.2, 1, 6, and 12 hour. Tartrate Resistant Acid Phosphatase (TRAP) staining was performed. Then, western blot analysis was performed to detect p38 MAPK and phosphorylated-p38 MAPK. Resulted protein bands were quantified and statistically analyzed.RESULTS: Under induction of 20 ng/mL RANKL and 1 ng/mL TNF-α, RAW-D cells were successfully differentiated into TRAP+ osteoclast-like polynuclear cells. Under treatment of 20 ng/mL of RANKL and 1 ng/mL of TNF-a for 0.2 or 1 hour, significant (p=0,000, T test) increment of phosphorylated p38 MAPK was observed as compared with control. Pretreatment of 10 μg/mL caffeic acid significantly (p=0.000, T test) suppressed the 20 ng/mL of RANKL and 1 ng/mL of TNF-a-induced phosphorylation of p38 MAPK.CONCLUSION: RANKL and TNF-a are potent osteoclastogenesis inductors in RAW-D cells, meanwhile caffeic acid could inhibit the RANKL and TNFa-induced osteoclastogenesis through p38 MAPK.KEYWORDS: caffeic acid, osteoclastogenesis, RANKL, TNF-a, p38, MAPK, RAW-D cells
Comparison of Tumor Necrosis Factor-α Level in Coronary Artery Disease and Coronary Slow Flow of Thrombolysis in Myocardial Infarction Muhammad Diah; Rahmawati Rahmawati; Aznan Lelo; Zulfikri Muhktar; Dharma Lindarto; Ferry Sandra
The Indonesian Biomedical Journal Vol 11, No 3 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i3.826

Abstract

BACKGROUND: Tumor necrosis factor (TNF)-α, an important primary pro-inflammatory cytokine, has a crucial role in the pathogenesis of atherosclerosis. Since the pathophysiological mechanism of coronary slow flow (CSF) is not fully understood, we investigated the level of TNF-α in coronary artery disease (CAD), CSF and healthy subjects.METHODS: This study was conducted in cross-sectional design involving 16 CAD, 18 CSF and 18 healthy subjects. Coronary angiography was recorded at the left anterior oblique, cranial, right anterior oblique, caudal, and horizontal positions. The flow in coronary arteries of the subjects were assessed using Thrombolysis in the Myocardial Infarction (TIMI) frame count method. Peripheral blood-derived serum was collected and level of TNF-α was determined by using highly sensitive enzymelinked immunosorbent assay (ELISA).RESULTS: No significant difference in level of TNF-α in CAD, CSF and healthy subjects (2.72±2.64 pg/mL, 1.88±0.8 pg/mL, 1.64±0.35 pg/mL, respectively) (p=0.087). In addition, there was no correlation between the concentration of TNF-α and TIMI frame count (r<0.2, p>0.05).CONCLUSION: There was no significant difference of TNF-α level in CAD, CSF and healthy subjects. In addition, there was no correlation between the TNF-α level with TIMI frame count as well. Nevertheless, further clinical studies with more subjects are needed.KEYWORDS: TNF-alpha, coronary artery disease, coronary slow flow 
Momordica charantia L. Fruit Fractions inhibit Malondialdehyde Level and Regenerate Hepatic Damage of Hyperglycemic Rats Parawansah Parawansah; I Putu Sudayasa; Andi Noor Kholidha Syarifin; Amirudin Eso; Nuralifah Nuralifah; Wa Ode Siti Rahayu Fathanah; Ferry Sandra
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.963

Abstract

BACKGROUND: Chronic hyperglycemia causes an increase of free radical production and in longterm, the hyperglycemia increases oxidative stress. Among medicinal plants, Momordica charantia L. fruit has been known to overcome hyperglycemia. However, role of M. charantia L. fruit on oxidative stress is not well understood. Therefore, current study was conducted to investigate the effect of M. charantia L. fruit extract on malondialdehyde (MDA) level and hepatic damage in hyperglicemic rat model.METHODS: Twenty five white rats (Rattus novergicus) were induced with Streptozotocin (STZ) and treated with/without glibenclamide, sodium carboxymethyl cellulose (Na-CMC), or M. charantia L. fruit ethanol/ethyl acetate/ n-hexane fraction. After the treatment, rat’s livers were collected and separated for histopathological examination and MDA analysis.RESULTS: The MDA level average of rats before the STZ induction was 1.37 μg/mL. MDA level average was markedly increased (23.85 μg/mL) in rats induced with STZ and treated with Na-CMC merely. The MDA level average of STZ-induced glibenclamide-treated rats was 3.12 μg/mL. Meanwhile, the MDA level averages of STZ-induced M. charantia L. fruit ethanol, ethyl acetate and n-hexane fractions-treated rats were 14.95, 8.98 and 5.37 μg/mL, respectively. The histopathology results of this study showed that adipocytes, dilated sinusoids and central vein thickening were mostly observed in STZ-induced Na-CMC-treated rats. Meanwhile, the STZ-induced ethanol/ethyl acetate/n-hexane fraction-treated rats did not exhibitthose expressions.CONCLUSION: M. charantia L. fruit fractions inhibit the MDA level average in liver tissue and regenerate hepatic damage of STZ-induced rats, especially the n-hexane fraction which could be a potential hepatic antioxidant and regenerative agent.KEYWORDS: Momordica charantia L., malondialdehyde, oxidative stress, hyperglycemia, diabetes mellitus
Free Radical Scavenging and Alpha/Beta-glucosidases Inhibitory Activities of Rambutan (Nephelium lappaceum L.) Peel Extract Wahyu Widowati; Maesaroh Maesaroh; Nurul Fauziah; Pande Putu Erawijantari; Ferry Sandra
The Indonesian Biomedical Journal Vol 7, No 3 (2015)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v7i3.180

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BACKGROUND: Diabetes mellitus (DM) is associated with oxidative reaction and hyperglycemic condition. Human body has an antioxidant defense system toward free radical, but overproduction of free radical causing imbalance condition between the free radical and the antioxidant defense in the body that lead to several diseases, including DM. Glucosidase is an enzyme that hydrolize carbohydrates causing increase of blood glucose level, so by inhibiting this enzyme blood glucose level in plasma could be effectively decreased. Rambutan (Nephelium lappaceum L.) peel has been reported to have many potential roles, such as antioxidant and anti-glycemia. Therefore our current study was conducted to evaluate possible effectivity of Rambutan peel to scavenge free radical and to inhibit α- and β-glucosidases. METHODS:Rambutan peel extraction (RPE) was performed based on maceration method. Geraniin was used as control. For antioxidant study, 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging test was performed. For glucosidase inhibitory activity study,  α- and β-glucosidases inhibitory activity tests were performed. Results were analyzed for median of Inhibitory Concentration (IC50).RESULTS: The scavenging activity of RPE was comparable with Geraniin. Meanwhile, the α-glucosidase inhibitory activity of RPE was higher than the one of Geraniin. The α-glucosidase-inhibitory-activity IC50 of RPE and Geraniin were 0.106±0.080 μg/ml and 16.12±0.29 μg/ml, respectively. The β-glucosidase inhibitory activity of RPE was also higher than the one of Geraniin. The β-glucosidase-inhibitory-activity IC50 of RPE and Geraniin were 7.02±0.99 μg/ml and 19.81±0.66 μg/ml, respectively.CONCLUSION: Since RPE showed comparable free radical scavenging activity with Geraniin and higher α- and β-glucosidases inhibitory activities than Geraniin, RPE could be suggested as a promising antioxidant and antiglycemic agent. KEYWORDS: Nephelium lappaceum L., rambutan, hypoglycemic, antioxidant, free radical, diabetes mellitus, glucosidase, DPPH
Immunohistochemical Expression of EGFR, NF-kB and Cyclin D1 in Sinonasal Inverted Papilloma and Squamous Cell Carcinoma Pudji Rahaju; Rio Auricknaga Kintono; Ahmad Dian Wahyudiono; Arif Satria; Ferry Sandra
The Indonesian Biomedical Journal Vol 12, No 3 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i3.1172

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BACKGROUND: Sinonasal inverted papilloma (SIP), a benign epithelial growth in the sinonasal region with epidermoid epithelial transformation, has been known for its invasiveness, recurrency, and its link with malignancy. Meanwhile sinonasal squamous cell carcinoma (SSCC) is an epithelial malignancy on squamous cells from the sinonasal region. Epidermal growth factor receptor (EGFR), Nuclear Factor kB (NF-kB), and Cyclin D1 are factors those might play important role in proliferation of SIP and SSCC. This research was conducted to investigate the expressions of EGFR, NF-kB and Cyclin D1 in SIP and SSCC.METHODS: A cross-sectional study by examining the EGFR, NF-kB, and Cyclin D1 immunohistochemical expressions of SIP and SSCC was conducted. Subjects whose blocks were used in this research, were diagnosed as SIP and SSCC at the Otorhinolaryngology-Head and Neck Surgery Clinic, Dr. Saiful Anwar General Hospital. Samples were selected, processed for inmmunohistochemistry, evaluated and statistical analyzed.RESULTS: Twenty-four SIP and 9 SSCC subjects with their paraffin blocks were selected. Clear immunohistochemical expressions of EGFR, NF-kB, and Cyclin D1 were observed for both SIP and SSCC. Significantly higher immunostaining levels of EGFR (45.6%, p=0.001) and NF-kB (42.2%, p=0.013) were observed in SSCC. Immunostaining levels of EGFR vs. NF-kB were moderately correlated (p=0.03, r=0.437), while the immunostaining levels of NF-kB vs. Cyclin D1 were strongly correlated (p=0.002, r=0.602).CONCLUSION: Expression of EGFR and NF-kB in SSCC were higher than the EGFR and NF-kB expression in SIP, suggesting that EGFR and NF-kB play important role in sinonasal malignancy.KEYWORDS: sinonasal, inverted papilloma, SCC, EGFR, NF-kB, Cyclin D1
Macerated-Pineapple Core Crude Extract-derived Bromelain Has Low Cytotoxic Effect in NIH-3T3 Fibroblast Dewi Liliany Margaretta; Angliana Chouw; Yanni Dirgantara; Melanie Sadono Djamil; Ferry Sandra
The Indonesian Biomedical Journal Vol 7, No 2 (2015)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v7i2.75

Abstract

BACKGROUND: Bromelain is a sulfhydryl proteolytic enzyme that can hydrolyze protein, protease or peptide. Bromelain can be found in pineapple stem, fruit and core. Bromelain is composed of 212 amino acid residues with cysteine-25 forming a polypeptide chain that can hydrolyze peptide bonds by H2O. In medicine, bromelain has been developed as antibiotic, cancer drug, anti-inflammatory agent and immunomodulator. In dentistry, bromelain has potential to reduce plaque formation on the teeth and to irrigate root canal.METHODS: Pineapple core was dried for 3 days to get simplicia. Then simplicia was extracted with water solvent for 24 hours. After that, the macerated-pineapple core crude extract-derived bromelain (PCB) was separated by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue (CBB) staining to ensure the presence of bromelain. In cytotoxic test, NIH-3T3 fibroblast cultures were treated with extracts in various concentrations to for 24 or 48 hours. Number of fibroblasts was calculated using 3-(4,5-dimethylthiazol-2- yl)-2,5-Diphenyltetrazolium bromide (MTT) assay.RESULTS: Pineapple core extraction using maceration method produced relative high yield (concentration: 1.5424 g/mL) of bromelain, which was confirmed by CBB staining results with the molecular weight of 33 kDa. Based on cytotoxic test results of PCB on NIH-3T3 fibroblasts, 24-hours-incubation LD50 was 95.7 g/L, while 48-hours-incubation LD50 was 51.1 g/L.CONCLUSION: PCB has low cytotoxic effect in NIH-3T3 fibroblasts.KEYWORDS: bromelain, pineapple, extract, cytotoxic, MTT
Co-Authors Abdul Gani Soulisa Abdul Gani Soulisa Achmad Guntur Hermawan Suryo Adji Achmad Kemal Harzif Addin Trirahmanto Ade Prijanti Dwisaptarini Adriana Todingrante Afiana Rohmani Ahmad Dian Wahyudiono Ahmad Kurnia Ahmad R. Utomo Ahmad R. Utomo Aida Fitri Nazillah Al Mazida Fauzil Aishaqeena Alfred Pakpahan, Alfred Ali Sulaiman Alida Roswita Harahap Alpha Fardah Athiyyah Amalia Isnaeni, Nur Amelia Astriani Putri Aminuddin Aminuddin Amirudin Eso Anang Endaryanto Andayani, Lia H. Andi Asadul Islam Andi Wijaya Andi Wijaya Andon Hestiantoro Andri Sutanto Andrian Nova Fitri Andrijono Andrijono Andy Darma Andy Wirahadikusumah Anggraeni, Rezky Angliana Chouw Angliana Chouw Angliana Chouw Angliana Chouw Ani Retno Prijanti Anie Apriani Anna Fuji Rahimah Annisa Amalia Annisa Putri Ariyani Annistri, Savrinda Anwar Santoso Aprisia, Kartika Dwi Ardian Rizal Aria Kekalih Arief Boediono Ariesanti, Yessy Arif Kurniawan Arif Santoso Arif Satria Arimaswti Arimaswati Ariyani, Annisa Putri Armelia Sari Arum Kartikadewi Arya Nur Ihsan Aryani Hayuningtyas, Ria Aryu Candra Asmarinah Asmarinah Ayasha Hajjar Audreyandra Wahid Ayu Rahadiyanti Ayunita Tri Wirattami Aznan Lelo Bambang Sutrisna Bambang Sutrisna Bastian Nova Benyamin Lukito Bethy Suryawathy Hernowo Bianda Claresta Bianda Claresta Blanca Sarizta Boedi Oetomo Roeslan, Boedi Oetomo Boenjamin Setiawan Britanto Dani Wicaksono Budi Utomo Budiono, Bernardus Parish Bukhari, Agussalim Caecilia Caroline Aliwarga Caesary Cloudya P Caesary Cloudya Panjaitan Cahyono Kaelan Camy F. Surjadi Caroline T. Sardjono Caroline Tan Sardjono Caroline Tan Sardjono Chandra Risdian Chandra Risdian Chantika Amardhia Paramita Putri Ciptadhi Tri Oka Binartha Ciptadhi Tri Oka, Ciptadhi Tri Cloudya P, Caesary Cloudya Panjaitan, Caesary Dea Jane Sungkono Denni Joko Purwanto Devi Nisa Hidayati Deviyanti Pratiwi Dewi Liliany Margaretta, Dewi Liliany Dewi Lilyani Margaretha Dewi Ranggaini Dewi, Dian Andriani Ratna Dhaniar, Afifah Yumna dharma Lindarto, dharma Diah Savitri Ernawati Dian R Laksmitawati Dian Ratih Laksmitawati Diana Nur Afifah, Diana Nur Dina Keumala Sari Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Djoko Wahono Soeatmadji Dominica Dian Saraswati Sumantri Dwi Davidson Rihibiha Dwifulqi, Hernindya Edi Handoko Eka Seftiana Indahsari Elina Ardiani Sidharta Elisabeth Pricilia Sunata Elline, Elline Ellyza Nasrul Elvita Rahmi Daulay Em Yunir, Em Enny Probosari Enos Tangke Arung, Enos Tangke Ergia Latifolia Eriawan Agung Nugroho, Eriawan Agung Erizal Sugiono Erlina Sih Mahanani, Erlina Sih Ervi Afifah Fahriza Abid Sonia Farhat, Farhat Farid Sastra Nagara Farid Sastra Nagara, Farid Sastra Fatmawati Fatmawati Fauzia Purnamasari Febriyeni, Febriyeni Felix Wong Fibryanto, Eko Fillah Fithra Dieny Fitriasih Fitriasih Fitriyadi Kusuma Franciscus D. Suyatna Frans D. Suyatna Frisca Frisca FX Budhianto Suhadi George Mathew Gita Pratama Gwendy Aniko Hadrian, Gary Halim, Johni HANA RATNAWATI Hariyono Winarto Harry Akza Putrawan Harry Murti Harryanto Wijaya Harryanto Wijaya Hartono, Tiffany Harun Iskandar Hayatun Nufus Hayuningtyas, Ria A. Hayuningtyas, Ria Aryani Hendradi Surjotomo Hendy Setyo Yudhanto Heri Wibowo Herqutanto Herqutanto Hidajat, Boerhan Hilary Limen Huda Shalahudin Darusman I Gusti Made Reza Gunadi Ranuh I Putu Sudayasa Ibnu Suryatmojo Ichwan, Solachuddin Jauhari Arief Ida Parwati Idrus Alwi Ihsan Rizal, Muhammad Ikhsan Nur Arifin Ilham Jaya Patellongi Ilhamjaya Patellongi Indra Bachtiar Indradewa, Rhian Indrayadi Gunardi Indriyanti Rafi Sukmawati Indriyanti Rafi Sukmawati Ingrid Suryanti Surono Intan Masyfufah Hanim Irawan Yusuf Irawan Yusuf Irianiwati Widodo Irvan Septrian S P R Irvanyuni Japutri Ivet Suriapranata Jackson Jackson, Jackson James Handojo Jamhari Jamhari Janti Sudiono Janti Sudiono Janti Sudiono Janti Sudiono Janti Sudiono Jasmine Shafira Jasmine Shafira, Jasmine Jeanne A. Pawitan Jeanne A. Pawitan Jenifer Christy Hadimartana Jennifer Putri Jessica Nathalia Chandra Jessica Nathalia Chandra, Jessica Nathalia Joceline Setiawan Johan Arief Budiman Juliani Hardja Buntara, Magdalena Juminten Saimin Junita Briskila Jusak Nugraha Jusuf Rachmat Karina Febriani Hudono Karyono Mintaroem Kent Wijaya Setiawan Ketherin Ketherin Ketherin Ketherin Ketherin Ketherin Ketherin, Ketherin Khairun Nisa Kharima Abdullah Kharima Abdullah, Kharima Khilmi Ainun Nadliroh Kirana, Farenza Okta Komariah Komariah Komariah Komariah Komariah Komariah Kusuma, Yohanna Laifa Annisa Hendarmin Laila Nuranna Lee, Kyung H. Lee, Kyung Hoon Lelyana, Shelly Leo Alberto Porjo Lia Hapsari Andayani Lidya Imelda Laksmi Linda Lison Linda Sari Sembiring Livia Alvita Liza Meutia Sari Louise Anastasya Luki Astuti Luthfiatul Khusna M Novo P Lubis Maesaroh Maesaroh Makmun, Armanto Mansyur Arif Mansyur Arif Marcellus Simadibrata Maria Celinna Maria Celinna Maria Evi Novianti Maria Verena Emeralda Marshel Tendean Marshel Tendean MARTHA ARDIARIA Maulanisa, Sinta C. Maurin Merlina Meita Hendrianingtyas Melanie S Djamil Melanie Sadono Djamil Melanie Sadono Djamil Melanie Sadono Djamil Melanie Sadono Djamil, Melanie Sadono Melina Setiawan Melinia Melinia Meta Ariyani Sidharta Meta Ariyani Sidharta, Meta Ariyani Mirta Hediyati Reksodiputro Mohammad Aris Widodo Mohammad Aris Widodo Mohammad Sadikin Mohammad Sadikin Monica Andajana Muhamad Rizki Fadlan Muhammad Diah Muhammad Hamdan Muhammad Ihsan Rizal, Muhammad Ihsan Muhammad Novo Perwira Lubis Muharam, Muharam Muharam, Raden Mulyawati, Sufiah Asri MUTMAINNAH, FITRI Nadhia Sari Afiana Nadhia Sari Afiana, Nadhia Sari Nadiah, Najla Nadya Saputri Halim Najla Nadiah Naliani, Silvia Nani Maharani Nanik Setjowati Nasrul Zubir Neni Susilaningsih Nenni Pratiwi Nova Adrian Noviasti Rahma Utami Novriansyah, Robin Nur Ahmad Tabri Nur Indah Purnamasari Nur Zam Zam Nuralifah Nuralifah, Nuralifah Nurani Hayati Nuri Dyah Indrasari Nurjannah Lihawa Nurrani Mustika Dewi Nurrani Mustika Dewi Nurrani Mustika Dewi, Nurrani Mustika Nurul Fauziah Nyoman Suci Widiastiti Oetomo Roeslan, Boedi Olivia Nauli Komala P Lubis, M Novo Pande Putu Erawijantari Pang, Tiffany Parawansah Parawansah Pendrianto Pendrianto Peter Kabo Peter Sugita Pieri Kumaladewi Prasetya, David Eka Pratitis, Visi Endah Primariadewi Rustamadji Primariadewi Rustamadji Pudji Rahajoe Pudji Rahaju Pukovisa Prawiroharjo, Pukovisa Putri Y Suyanto Putri Y Suyanto, Putri Y Putri, Indah Aprianti Quan Yong Tang Rachma Purwanti Rafika Indah Paramita Rahmawati Rahmawati Rahmawati, Dicha Yuliadewi Rahmi Amtha Rahmi Amtha Rahmi Annisa Ramadhan Karsono Ramadhan Ramadhan Rambu Beppy Hamuaty Rambu Beppy Hamuaty, Rambu Beppy Ranggaini, Dewi Ratna Akbari Ganie Respati, Danendra Rakha Putra Retno Asih Setyoningrum Reynaldo Lambertus Rifqi Fauzan Hakim Riko Nofrizal Rio Auricknaga Kintono Riris L. Puspitasari Riris L. Puspitasari Rita Lahirin Rita Lahirin, Rita Ritawaty Ritawaty Rizal Rizal Rizkia Milladina Hidayatulloh Roihatul Mutiah Ronald Winardi Kartika Rosalina Tjandrawinata Roya Khosravi-Far Roya Khosravi-Far Ryco Giftyan Ardika Saleha Sungkar Saptawati Bardosono Saraswati, Jaya Sari, Armelia Sarwono Waspadji Scania, Alifah Evi Seila Arumwardana Septrian S P R, Irvan Setiawan, Kent Wijaya Siti Mega Komariyah SITI NURUL HIDAYATI Soehartono Soehartono Solachuddin Jauhari Arief Ichwan Sonar Soni Panigoro Soulisa, Abdul Gani Soulissa, Abdul G. Sri Mutya Sekarutami Stefani, Rosita Steffi Kurniawan Steward Hadi Steward Steward Sudarmo, Subijanto Marto Suhendro Suhendro Sukartini, Ninik Susi Tjahyani Susy Tjahyani Suzzana Immanuel Syafiqa Amelia, Dinda Syafira Noor Pratiwi Syakib Bakri Syanindita Puspa Wardhani Syarifin, Andi Noor Kholidha Tadahiko Iijima Tadahiko Iijima Taramalinda, Elizabeth Yuliani Taslim, Nurpudji Astuti Tatsushi Muta Teguh Santoso Teguh, Sharren Timotius Andi Kadrianto Tjandra, Kevin Christian Tjandrawati Mozef Tjandrawati Mozef Tjandrawati Mozef Todung Silalahi Toshio Kukita Toshio Kukita Trijani Suwandi Trisfilha, Pretty Tugas Ratmono Tulandi, Togas Uleng Bahrun Victor Reiner Sutanto, Aldrich Virgi Saputra Wa Ode Siti Rahayu Fathanah WAHYU WIDOWATI Wahyu Widowati Wahyu Widowati Wahyu Widowati Wibi Riawan Widiastiti, Nyoman Suci Widya Wulandari Widyastuti, Wiena Wiena Widyastuti Wijaya, Haryanto William Yusuf, Timotius Winarko Luminturahardjo Wirsma Arif Harahap Yanni Dirgantara Yanni Dirgantara Yanni Dirgantara Yanuarita Tursinawati Yefta Moenadjat Yenti Purnamasari Yohanna Feter Yohanna Feter, Yohanna Yora Nindita Yudi Her Oktaviono, Yudi Her Yuliani Taramalinda, Elizabeth Yusra Yusra Yusrina Lukitasari Yuyus Kusnadi Zulfikar Lubis Zulfikri Muhktar