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Effect of Specific Formulated Feed to Alter the Glucose, Triglycerides and Total Cholesterol Level in Rat (EFEK FORMULASI PAKAN TIKUS TERHADAP PERUBAHAN KADAR GLUKOSA, TRIGLISERIDA DAN KOLESTEROL TOTAL) Jihan Mudrika Rahmi; Ronny Lesmana; Hana Goenawan; Setiawan Setiawan; Vita Murniati Tarawan; Okta Wismandanu; Unang Supratman
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (997.945 KB) | DOI: 10.19087/jveteriner.2019.20.2.179

Abstract

Understanding the molecular mechanism of type 2 diabetes mellitus using human sample is very difficult, therefore utilization of animal model can be the solution for studying diabetes mellitus. However, there is still limited option of the animal model that can describe the process of type 2 diabetes mellitus. The aim of this study is to develop a formulated feed with increased glucose, triglycerides, and total cholesterol as characteristics of type 2 diabetes mellitus. This study was done using a descriptive method and experimental study design for 8 weeks. Total 9 male and 12 female Wistar rats were randomly divided into 3 groups: 1 control group and 2 treatment group (formula 1: high carbohydrate, formula 2: high fat). The increase of delta mean body weight was higher in the group given formula 1 for both male and female rats (f: 52g, m: 71.67g). The glucose level was increase from 4th- 8th week in both gender of rats and higher in the group given formula 1 of female rats (4W: 221.13 mg/dl; 8W: 249.83 mg/dl). The triglycerides level was increased from 4th to 8th week and higher in group given formula 2 either male (4W: 37.42 mg/dl; 8W: 58.92 mg/dl) but not in female rats (4W: 76.78 mg/dl; 8W: 71.22 mg/dl). The cholesterol total level also increased in male (4W: 93.05 mg/dl; 8W: 282.5 mg/dl) and female (4W: 101.58 mg/dl; 8W: 227.17 mg/dl) rats and higher in the group given formula 1. In conclussio, either high carbohydrate or high fat pellet showed potential capability to increase the glucose, triglyceride and total cholesterol level especially in female rats.
DMBA-induced Modulate Estrogen Receptors α and β Breast Cancer’s Animal Model Aziiz Mardanarian Rosdianto; Ahmad Kurniawan; Julia Windi Gunadi; Isa Mahendra; Iwan Setiawan; Hanna Goenawan; Nova Sylviana; Yuni Susanti Pratiwi; Mas Rizky Anggun Adipurna Syamsunarno; Roro Wahyudianingsih; Unang Supratman; Ronny Lesmana
Majalah Kedokteran Bandung Vol 54, No 1 (2022)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v54n1.2479

Abstract

The high incidence of breast cancer cases in the world requires the use of applicative methods. The 7,12-dimethylbenz(a)anthracene (DMBA) induced breast cancer animal model is a widely used chemical-induced animal models for research on breast cancer. However, the molecular mechanism related to DMBA induction remains unclear. Good understanding on DMBA-induced animal models is crucial for studies related to future breast cancer treatments as animal models will provide a deeper understanding of anticancer medication, specifically those aimed for treating breast cancer. The aim of this study was to develop an DMBA-induced animal model for breast cancer. This study used female Wistar rats injected subcutaneously with DMBA as a carcinogen-induced agent (20 mg/kg) to induce tumor. Rat tumors were then evaluated and breast appearance was observed weekly, starting from day 28th after DMBA injection. Breast cancer tissue was then sampled and stored at -80°C until it was used for western blot and histological study. This study indicated that DMBA induced cancer in female Wistar rat’s breasts, and cytoplastic cells and lung metastatic was identified macroscopically and histopathologically. The metabolic sign was observed in the lung and breast sections. Interestingly, the DMBA induction in this study does not only induce organ cancers but also induces estrogen receptors and stimulates signaling of estrogen receptors α (ERα), ERβ, and Akt.
The Effect of Nutmeg Seed (M. fragrans) Extracts Induces Apoptosis in Melanoma Maligna Cell’s (B16-F10) Susianti Susianti; Ronny Lesmana; Supriatno Salam; Euis Julaeha; Yuni Susanti Pratiwi; Nova Sylviana; Hanna Goenawan; Ahmad Kurniawan; Unang Supratman
The Indonesian Biomedical Journal Vol 13, No 1 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i1.1424

Abstract

BACKGROUND: Nutmeg (Myristica fragrans H.), one of native plants of Maluku Indonesia, has long been used as traditional medicines especially to treat tumors, externally to treat skin infections. M. fragrans also has important biological activities as anticancer. However, antimelanoma activity of M. fragrans remains unknown. The aim of this study is to compare M. fragrans extracts as anticancer on melanoma B16-F10 cells by inducing apoptosis.METHODS: M. fragrans seed was extracted with ethanol then fractionated with n-hexane, ethyl acetate, and n-butanol. B16-F10 melanoma cells were cultured and treated with various doses and tested using resazurin reduction assay. Apoptosis signalling via caspase-3 was measured by using western blot.RESULTS: The extract and fractions of M. fragrans reduced viability of cells with IC50 value for ethanol extract 21.83 µg/mL, ethyl acetate fraction 21.66 µg/mL, n-hexane fraction 47.53 µg/mL, and n-butanol fraction >1,000 µg/mL. The active fraction of ethyl acetate induced apoptosis via caspase-3 proteins similar with cisplatin as positive control in B16-F10 cells at 10 hours treatment.CONCLUSION: Taken together, M. fragrans ethyl acetate fraction has the highest IC50 than n-hexane and n-butanol fractions that significantly inhibited B16-F10 cell proliferation by inducing apoptosis via caspase-3. It provides the insight that it has the most potential activity as a chemopreventive agent for addressing melanoma skin cancer.KEYWORDS: M. fragrans, apoptosis, fraction, melanoma
Ethionamide Alters Thyroid Receptor Gene Expression in Rats' Muscle Ronny Lesmana; Setiawan Setiawan; Ferdyan Efza; Yuni Susanti Pratiwi; Gilang Muhamad Nur Iqbal; Hanna Goenawan; Nova Sylviana; Unang Supratman
The Indonesian Biomedical Journal Vol 12, No 3 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i3.1138

Abstract

BACKGROUND: Ethionamide usage as one of the drug regimens still becomes a challenge due to high numbers of patients developing hypothyroid. Ethionamide had been associated with the inhibition of thyroid hormone (TH) synthesis and interestingly, ethionamide (C8H10N2S)-induced hypothyroidism is supported by its similar structure with thioamides, propythiouracil (C7H8N2S). However, hypothyroidism is not solely caused by its production, it could be caused by signaling alteration. Therefore, knowing that important TH action is determined via genomic pathway, alteration of this receptor could bring serious clinical problem. Unfortunately, there is limited study about the regulation of ethionamide and its connection on TH genomic signaling especially thyroid hormone receptor (TR) gene expression in soleus, gastrocnemius and cardiac muscle.METHODS: Thirty-eight rats were divided into control, ethionamide and propylthiouracyl groups. After 12-week treatment, rat were sacrificed, then gastrocnemius, soleus and cardiac muscles were dissected out, snap freezed using liquid nitrogen, and stored in -80oC until use. RNA was extracted and run for reverse transcription polymerase chain reaction (RT-PCR).RESULTS: In soleus muscle, ethionamide stimulated TR mRNA expressions and deiodinase compared to control group. In contrast, TRα1 gene expression was not affected by ethionamide administration. In gastrocnemius muscle, only TRβ1 gene and Dio2 gene expressions that were significantly increased compared to control group. In cardiac muscle, ethionamide significantly stimulated all the thyroid hormone receptor isoform and iodothyronine deiodinase gene expression compared to the control group.CONCLUSION: Long ethionamide treatment upregulates TR gene expressions and deiodinase in soleus and cardiac muscle, there is different expression pattern of soleus, gastrocnemius and cardiac muscle after ethionamide stimulation.KEYWORDS: ethionamide, hypothyroid, TRα1, TRα2, TRβ1, TRβ2
Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1 Dimpuulina Erna Mariati; Sunarjati Sudigdoadi; Ronny Lesmana; Astrid Feinisa Khairani; Julia Windi Gunadi; Vita Murniati Tarawan; Unang Supratman; Hanna Goenawan
The Indonesian Biomedical Journal Vol 13, No 1 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i1.1428

Abstract

BACKGROUND: Recently, coffee is widely used for preventing photoaging because of its antioxidant capacity. Among two kinds of coffee, robusta coffee has higher content of antioxidant such as chlorogenic acid (CGA) and caffeine. Researchs about robusta coffee bean effect on photoaging due to UVB radiation is still limited. Therefore, the aim of this study was to examine the effect of robusta extract cream (RE cream) on preventing wrinkle in mice induced by ultraviolet-B (UVB) radiation.METHODS: RE cream was made by mixing RE coffee with moisturizing cream in different concentration (10%, 20%, and 40%). Twenty-five male of Mus musculus Balb/c strain mice aged 4 weeks were divided into five groups; control group, UVB group, UVB + 10% RE group, UVB + 20% RE group, and UVB + 40% RE group. The UVB groups were given UVB radiation three times a week with an exposure duration of 100 seconds per time for ten weeks. At the end of the treatment, skin samples were excised and statined histologically, also were analyzed for their protein expression. Evaluation of wrinkles was carried out using the Bissete method before and after treatment. To evaluate the thickness of the epidermis, HE staining was performed, while masson Trichome staining was performed to determine the collagen content.RESULTS: RE cream-treated groups showed lower wrinkle score compared to the control group. Furthermore, in UVB + 10% RE group, the RE cream application reduce wrinkle formation. In UVB + 10% RE group and UVB + 20% RE group, the RE cream application increased epidermal thickness and collagen content (p=0.00). While collagenase, matrix metalloproteinase-1 (MMP-1) expression was lower in UVB + 20% RE group compared to the UVB group (p<0.05), however the MMP1 expression in UVB + 40% RE group was higher than other treatment group.CONCLUSION: RE cream prevents wrinkle by maintaining epidermal thickness and collagen contain. RE cream also decreases MMP-1 expression in mice.KEYWORDS: coffee, collagen, MMP-1, robusta, wrinkle
An Isoflovonoid, Warangalone from the Stem Bark of Dadap Ayam (Erythrina variegata) Tati Herlina; Nasrudin Nasrudin; Unang Supratman; Anas Subarnas; Supriyatna Sutardjo; Hideo Hayashi
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.012 KB)

Abstract

In the course of our continuing search for novel paralytic compound from Indonesian plants, the methanol extract of the stem bark of Erythrina variegata (Leguminosae) showed significant paralytic activity against the third instar larvae of silkworm (Bombyx mori). The purposes of this research were isolation and structural elucidation of paralytic compound from the stem bark of E. variegata. Using the paralytic activity following the separation, the methanol extract was separated by combination of column chromatography to yield prenylisoflavone, warangalone. The chemical structure of warangalone was identified based on spectroscopic evidence and comparison with the previous reported. The paralytic activity of warangalone showed weak activity against the third instar larvae of silkworm (B. mori).
Insecticidal Bufadienolides from The Leaves of Kalanchoe daigremontiana (Crassulaceae) Wawan Hermawan; Rani Maharani; Sofa Fajriah; Revan Hardiawan; Unang Supratman
Jurnal ILMU DASAR Vol 11 No 2 (2010)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.04 KB)

Abstract

Kalanchoe is the biggest genera of Crassulaceae family and distributed in tropical and subtropical regions. This genera is found to be a rich source of biologically active natural products such as triterpenes, flavonoids and steroids. As a part of our continuing search for novel insecticidal compounds from Indonesian Kalanchoe plants, we examined Kalanchoe daigremontiana collected from Bandung region, West Java, Indonesia. The methanolic extract of the dried leaves of K. daigremontiana was concentrated and extracted with methylene chloride The methylene chloride extract exhibited an insecticidal activity toward silkworms. The methylene chloride extract was partitioned between n-hexane and methanol containing 10% water. The active lower layer was extracted with ethyl acetate. By using the insecticidal activity to follow the separations, the ethyl acetate fraction was separated by combination of column chromatography on Kieselgel 60 and ODS to afford two insecticidal bufadienolides 1 and 2. The structures of these compounds were elucidated based on spectroscopic analysis (UV, IR, NMR, 2D-NMR) and comparison with those related data previously reported. In this paper, the isolation, structural elucidation, and insecticidal activities against the third instar larvae of silkworm will be described.
Triterpenoid Pentacyclic Antimalarial Activity from the Leaves of Erythrina variegata Tati Herlina; Unang Supratman; Anas Urbanas; Supriyatna Sutardjo; Noor Rain Abdullah; Hideo Hayashi
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.786 KB)

Abstract

In the course of our continuing research for novel antimalarial agent from Indonesian plants, the methanol extract of the Erythrina variegata leaves showed significant antimalarial activity against Plasmodium falciparum strain K1 in vitro with IC50 6.8 μg/mL. The methanol extract was separated by using variety of chromatography techniques. The chemical structure of an antimalarial compound was determined on the basis of spectroscopic evidence and compared to previous data then this compound is identified as a pentacyclic triterpenoid oleane derivative, namely 3,22,23-trihydroxy-oleane-12-ene. The pentacyclic triterpenoid, 3,22,23-trihydroxy-oleane- 12-ene showed antimalarial activity against 3D7 and K1 strains with IC50 4.3 μg/mL and 24 μg/mL, respectively. These results strongly suggested that E. variegata is a promising sources of antimalarial agents.
In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant Tati Herlina; Unang Supratman; Anas Subarnas; Supriyatna Sutardjo; Suseno Amien; Hideo Hayashi
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp286-290

Abstract

Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards breast cancer cell T47D. In the course of our continuing search for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of E. variegata showed significant anti-cancer activity against breast cancer cell T47D in vitro using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure of an anti-cancer compounds were determined on the basis of spectroscopic evidence and comparison with the previously reported and identified as an erythrina alkaloid (1) and isoflavonoid (2). Compounds (1-2) showed anti-cancer activity against breast cancer cell T47D used with IC50 of  1.0 and 3.3 µg/mL, respectively. This results strongly suggested that E. variegata is promising sources for anti-cancer agents. Keywords: Anti-cancer, Erythrina variegata, Leguminoseae
Flavonoid Compounds from the Herb of Krokot (Lygodium microphyllum) and their antioxidant activity against DPPH Hadi Kuncoro; Kindi Farabi; Laode Rijai; Euis Julaeha; Unang Supratman; Yoshihito Shiono
Journal of Mathematical and Fundamental Sciences Vol. 50 No. 2 (2018)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2018.50.2.7

Abstract

Six flavonoid compounds, kaempferol (1), quercetin (2), acacetin (3), quercetin-3-O-β-D-glucopyranoside (4), kaempferol-3-O-β-D-glucopyranoside (5), and isorhamnetin-3-O-β-D-glucopyranoside (6), were isolated from the methanol extract of the Lygodium microphyllum herb. The chemical structures of compounds 1-6 were identified on the basis of spectroscopic evidence and comparison with previously reported spectral data. Compounds 1-6 were isolated for first time from this plant. Their antioxidant activity against DPPH (2,2-Diphenyl-1-picrylhydrazyl) was evaluated. Among the compounds, quercetin (2) showed high antioxidant activity with IC50 values of 6.94 ± 0.03 μg/mL.
Co-Authors , ,. Melanie - Ruchiyat . Horizon . Susianti Ace Tatang Hidayat Ace Tatang Hidayat Ace Tatang Hidayat Ace Tatang Hidayat Achmad Zainuddin Achmad Zainuddin Ade Akbar Abdilla Ade Kania Ningsih Ade Kania Ningsih, Ade Kania Adel Zamri Agus Safari Agus Safari Agus Safari Agus Safari Agustini, Dewi Meliati Ahmad Darmawan Ahmad Kurniawan Ahmad Kurniawan Ahmad Ramdan Ahmad Yani Nelly Wahyuni Lia Destiarti Akhmad Darmawan Al Arofatus Naini Al Arofatus Naini Al Arofatus Naini Aldo Hartono Almas Widyana Alya Tsamrotul Amir M. Suruwaky Anas Subarnas Anas Urbanas Anastasya Firdausi Andi Rahim Andre A. Sonda Aneu Wahyuni Anjari, Intan Hawina Aprilia Permata Sari Aprilia Permata Sari Aprina, Lutfia Silva Ari Hardianto Ari Hardianto Ari Widiyantoro Arif Rahman Hakim Arif Rahman Hakim Arif Rahman Hakim Arif Rahman Hakim Arlette Setiawan Arlette Setiawan Arlette Setiawan Arlette Setiawan Arlette Setiawan Arlette Suzy Puspa Pertiwi Arto Yuwono Soeroto Asep Supriadin Asri Peni Wulandari Asri Peni Wulandari, Asri Peni Astrid Feinisa Khairani Aziiz Mardanarian Rosdianto Azmi Azhari Azmi Azhari, Azmi Azmi, Mohamad Nurul Benny Joy Betry Pujiastuti Budiman, Yudha P. C Hanny Wijaya Celcius Waranmaselembun Christina Marpaung Dadan Sumiarsa Danar Dono Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati, Darwati, Darwati Deden Indra Dinata Deden Indra Dinata, Deden Indra Desi Harnet Desi Harneti Desi Harneti Desi Harneti Putri Huspa Desi Harneti Putri Huspa Dewa G Katja Dewa Gede Katja Dewa Gede Katja Dewa Gede Katja Dikdik Kurnia Dimpuulina Erna Mariati Dini Oktaviani Dondin Sajuthi Dudi Runadi DUDI RUNADI Edi Suanto, Edi Edi Sukmana Edi Sukmana Edi Sukmana Elis Hastuti Elisabeth Krismayanti Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Erina Hilmayanti Erina Hilmayanti Erina Hilmayanti Erina Hilmayanti Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Euis Julaeha Evan Hadrian Ezatul Ezleen Kamarulzaman Fadlilah, Gina Faizah Maira Faizal Hermanto Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar Fauzi Abdullah Fajar, Mohamad Fajar, Mohamad Fani Rahma Yenita Farabi, Kindi Fathurachman Fathurachman Fauzan Zein Muttaqien Ferdyan Efza Ferry Ferdiansyah Sofian, Ferry Ferdiansyah Filza Yulina Ade Fizrul Indra Lubis Galih Bayu Pratama Galuga Sinalusur Sari Ghina Izdihar Gilang Muhamad Nur Iqbal Gunawan, Latifah Hadi Kuncoro Hadi Kuncoro Hana Goenawan Hanna Goeanawan Hanna Goenawan Harizon Harizon Harlia Harlia Hasbilla, Raihan Fathurrahman Hasnah Osman Hasnah Osman Hedi Paramita Herdanu Rizqullah Hersa Milawati Hersa Milawati Hersa Milawati Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hideo Hayashi Hilmayanti, Erina Huda, Muhammad Badrul Husein Hernadi Bahti Hutagaol, Ricson Pemimpin Ichsan Nurul Bari Ida Ayu Putu Sri Widnyani Ida Nur Farida Ida Nur Farida Ida Nurfarida Ihsan Rahadian Iis Intan Widiyowati Indri Indriyani Indriyani, Indri Inne Suherna Sasmita Intan Hawina Anjari Intan Rahmayanti Iqbal Musthapa Iqbal Wahyu Mustaqim Isa Mahendra Isramiharti Isramiharti Iwan Setiawan Iwan Setiawan Iwan Setiawan Jamaludin Al-Anshori Jihan Mudrika Rahmi Julia Windi Gunadi Julia Windi Gunadi Julinton Sianturi Julinton Sianturi Kadarusman Kansy Haikal Kansy Haikal Kautsari, Arsi Khadijah Awang Khairunnisa, Shofiyah Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khalijah Awang Khlaijah Awang Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kok Tong Wong Kristin Shinta Dewi Kusumiyati Laode Rijai Lilis Siti Aisyah Lilis Siti Aisyah Lilis Siti Aisyah Lilis Siti Aisyah, Lilis Siti Lindung Tri Puspasari Lindung Tri Puspasari M. S. Soedjanaatmadja Maira, Faizah Mantiri, Sisilia A Maryati Maryati Mas Rizky A.A. Syamsunarno Max R.J Runtuwene Max R.J Runtuwene Mayshah Purnamasari MEGANTARA, SANDRA Milawati, Hersa Moelyono Moektiwardoyo Mohamad Fajar Mohamad Fajar Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi bin Mohamad Taib Mohamad Nurul Azmi Mohamad Taib Mohamad Nurul Azmi Mohamad Taib Mohamed Ashraf Ali Mohd. Zaheen Hassan Mohd. Zaheen Hassan Muchlis, Handi Nugraha Muhamad Nurul Azmi Muhamad Nurul Azmi Muhammad Hanafi Muhammad Hanafi Muhammad Hanafi Muhammad Solehin Abd Ghani Muhammad Solehin Abd Ghani Murtihapsari . Mustaqim, Iqbal Wahyu Mustofa, Hidayat Nurul Muttaqin, Fauzan Zein Nadia Mohamed Yusoff Nadya Thufaila Nafiah, Mohamad Azlan Naini, Al Arofatus Nasrudin Nasrudin Nayla Haraswati Noor Rain Abdullah Noor Rain Abdullah Nova Sylviana Nova Sylviana Nulelasari Nurlelasari Nunung Kurniasih Nunung Kurniasih Nunung Kurniasih, Nunung Nur Insani Amir Nur Muhammad Miftah Nurabi Ferdiana Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Nurlelasari Okta Wismandanu Paramita, Hedi Ponis Tarigan Prasetyo, Wibowo Budi Pratama, Galih Bayu Prayudi Santoso Primahana, Gian Purbaya, Sari Purnama Purnama Purnama Purnama Purnama Purnama Purwoko, Agus Puspita Sari Putri, Ghesta Alifka Rahmawati Rahmawati Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Ratu Safitri Ray, Hamidie Ronald Daniel Rejeki, Purwo Sri Revan Hardiawan Richa Mardianingrum Ricson Pemimpin Hutagaol Rika Meliansyah RIKA MELIANSYAH Risyandi Anwar Riza Apriani Rizky Abdulah Rizky Abdullah Romundza, Febbry Ronauli Fitriana Ronny Lesmana Ronny Lesmana Roosje Rosita Oewen, Roosje Rosita Roro Wahyudianingsih Rudi Hendra Rurini Retnowati Rymond Jusuf Rumampuk Safri Ishmayana Safriansyah, Wahyu Salam, Supriatno Salam, Supriyanto Samuel San Parulian Sandra Amalia Riyadi Sari Purbaya Sari Purbaya Sari, Aprilia Permata Satwika Nandiwardhana Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Shabarni Gaffar Shabarni Gaffar Shiono, Yoshihito Sianturi, Julinton Sinaga, Siska Elisahbet Sisilia A Mantiri Siska Elisahbet Sinaga Siska Mulya Octavia Siska Mulya Octavia Siti Hani Pratiwi Sofa Fajriah Sofa Fajriah Sofa Fajriah Sofa Fajriah Sofa Fajriah Sofa Fajriah Sofa Fajriah Srikandi, Srikandi Steffi Triany Arnov Subekti Mauluddin Sudarjat Sudarjat Sunarjati Sudigdoadi Supriatno Supriatno Supriatno Salam Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriatno Supriyatna Supriyatna Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna Sutardjo Supriyatna, - Suseno Amien Susianti Susianti Susianti Susianti Susianti Susianti, Susianti Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Tati Herlina Teddy Budiyansyah Thaigarajan Parumasivam Tiara Prima Amalya Tjandrawati Mozef Toto Subroto Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Reksa Saputra Tri Reksa Saputra Tri Reksa Saputra, Tri Reksa Vicki Nishinarizki Vidia Afina Nuraini Vita Murniati Tarawan Vita Murniati Tarawan W.C. Taylor Wahyu Syafriansyah Wahyuni, Aneu Wawan Hermawan Wawan Hermawan Winda Sukmawati Winda Sukmawati Witriany Rayapratiwi Yeni Mulyani Yenny Febriani Yun Yenny Febriani Yun Yenny Febriani Yun Yenny Febriani Yun, Yenny Febriani Yeong Keng Yoon Yessi Permana Yoshihito shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yum Eryanti Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi, Yuni Susanti Yusup Hidayat