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Prevalence of Gastrointestinal Parasite on Cats in Shelter East Surabaya Akbar Wijaya Putra Purnama; Lucia Tri Suwanti; Hani Plumeriastuti; Endang Suprihati; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.926 KB) | DOI: 10.20473/jops.v3i2.16517

Abstract

The purpose of this study was to determine the prevalence of gastrointesatinal parasites in cats at Surabaya shelter. Eighty two samples of cat feces used in this study were taken from Griya satwa shelter and Kiro cat shelter. Feces samples were examined using the native, sedimentation and floating methods. Feces samples gastrointestinal parasite were identified by using a microscope with 400x and 1000x magnifications. Faces samples that are diagnosed positively if there is at least one of the three methods. Feces samples has observed, obtained prevalence rate of 43.9% or 36 positive samples from eighty two samples. The results of chi square analysis showed that p > 0.05, which showed that the parasites taken in the two shelters had clear differences in the prevalence of the gastrointestinal tract parasites. The parasites observed were Isospora sp., Ancylostoma sp., Toxocara cati and Toxocara leonina.
PROFIL PROTEIN TIROSIN KINASE DALAM SEMINAL PLASMA DOMBA MERINO DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS Tantri Nawangwulan; Sri Pantja Madyawati; Hani Plumeriastuti; Trilas Sardjito; Suherni Susilowati; Erma Safitri
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.142 KB) | DOI: 10.20473/ovz.v7i2.2018.91-95

Abstract

The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Semen was collected by artificial vagina and then centrifugated for 40 minutes at 4000 rpm to separated seminal plasma and spermatozoa. Protein was analyzed using SDS-PAGE, to separate each protein based on their molecular weight. The result showed that there were 13 protein bands in 3 seminal plasma samples with an average 149,63 kDa, 139,7 kDa, 114,97 kDa, 109,3 kDa, 97,33 kDa, 93,83 kDa, 86,23 kDa, 77,6 kDa, 64,6 kDa, 52,3 kDa, 41,93 kDa, 38,13 kDa, and 34,5 kDa. In conclusion, it is believed that tyrosine kinase located on the sixth band with molecular weight 93,83 kDa.
PENGARUH PEMBERIAN MADU TERHADAP STAGING SPERMATOGENESIS DAN SEL LEYDIG PADA MENCIT (Mus musculus) YANG DIINFEKSI Toxoplasma gondii Paraswita Eindah Fitri; Wurlina Wurlina; Sri Chusniati; Lucia Tri Suwanti; Mufasirin Mufasirin; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.345 KB) | DOI: 10.20473/ovz.v8i1.2019.10-16

Abstract

The aim of this research is to know about the staging of spermatogenesis and leydig cells in mice that injected by Toxoplasma gondii after giving honey as a preventive substance. This research used 25 mice in age 2-4 month with 20-30 weight. Mice were divided into 5 groups (P0, P1, P2, P3 dan P4). P0 as a control teratment were given aquades in peroral, P1 was a group given honey in doses I (0.08ml/0.2ml/day) during 14 days in peroral, P2 was a group that given infection by T.gondii in doses 103 in peritonial, P3 was given honey in doses I 0.08ml/0.2ml/day) then injected by T.gondii, and P4 was given honey in doses II (0.12ml/0.2ml/day) then injected by T.gondii. This research conducted in 18 days, in day 14th mice were given honey and the day 15th–18th mice injected by T.gondii doses 103. The data result was analyzed with Analysis of Variant (ANOVA) and cotinued with Duncan. Comparision statistical showed with SPSS statistic 20 for Windows. The result of analysis, P0 and P1 were significantly different (p<0.05), with the increase number of spermatogenic and leydig cells. P0 and P2 also significantly different, with the decrease number of spermatogenic and leydig cells. In P2 and P3 group were not significantly different (P>0.05), also in P4 group were not significantly different with P0 and P1. The results of this research showed that honey in doses II can maintain the number of sprematogenic and leydig cells as a preventive substance against T.gondii.
PENGARUH PEMBERIAN JAMUR Fusarium graminearum TERHADAP HISTOPATOLOGI TUBULUS SEMINIFERUS MENCIT (Mus musculus) Fierda Kabayo; Abdul Samik; Ismudiono Ismudiono; Tjuk Imam Restiadi; Soeharsono Soeharsono; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.727 KB) | DOI: 10.20473/ovz.v8i1.2019.54-60

Abstract

The aim of this research was to show the influence of seminiferous tubules histopathology of mice (Mus musculus) caused Fusarium graminearumexposure. This research was done in April-May 2017 in Microbilogy Laboratory Faculty of Science and Technology, Animal Laboratory, and Microbilogy Laboratory Faculty of Veterinary Medicine UniversitasAirlangga. This research used 20 male mice (Mus musculus) aged 6 weeks with 18-30 gram body weight. The mice devided into four groups: P0 given 0,25 ml Sodium chloride without Fusarium graminearum exposure by oral; P1 given 0,25 ml Fusarium graminearum exposure with dilution 102 by oral; P2 given 0,25 ml Fusarium graminearum exposure with dilution 103 by oral; and P3 given 0,25 ml Fusarium graminearum exposure with dilution 104 by oral. This treatment carried out for 21 days. Each milliliter dilution containing 228x106 spore for P1, 228x107 spore for P2, and 228x108 spore for P3. Then do the surgery and harvesting the testes then performed histopathological examination by scoring of seminiferous tubules. For data analyzing used non parametric difference Kruskall-Wallis and continued with Mann-Whitney. The result of this research was showed that decreased the spermatogenic cells in the process of spermatogenesis significant.
PENGARUH EKSTRAK DAUN PARE (Momordica charantia) TERHADAP FOLIKEL OVARIUM TIKUS (Rattus norvegicus) DALAM KONDISI HYPERGLYCEMIC Ganeswara Muharam Hazmi Rezady; Hani Plumeriastuti; Rimayanti Rimayanti
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.808 KB) | DOI: 10.20473/ovz.v8i1.2019.61-65

Abstract

The purpose of this study was to prove that the provision of pare leaf extract can reduce the process of follicular atrophy in the ovaries caused by hyperglycemic conditions. The total of this study was completed within 20 days, including the induction of Streptozotocin for 5 days, treatment for 14 days, and 1 day for ovarian collection. The K- group as a negative control without being induced by Streptozotocin and received no treatment. The K+ group as a positive control was only induced by Streptozotocin at a dose of 55 mg / Kg BW and D10 without treatment. The T1 group was induced by Streptozotocin at doses of 55 mg / Kg BW and D10 and treated with pare leaves extract (Momordica charantia) at a dose of 1.26 ml / Kg BW. T2 group was induced by Streptozotocin with dose of 55 mg / Kg BW and D10 and was treated with pare leaves extract (Momordica charantia) at a dose of 2.52 ml / Kg BW. Induction of Streptozotocin intraperitoneally and once-daily peroral treatment. The result showed that treatment group could make a significant difference with the positive control. Showing that amount of gap could make Momordica charantia to be alternative treatment for hyperglycemic condition.
Virgin coconut oil protected the diameter and the epithelium thickness of seminiferous tubules of mice (Mus musculus) from oral ethanol induction Vishantheni S Ravi; Soeharsono Soeharsono; Hani Plumeriastuti; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.34-40

Abstract

This study investigated the preventive effect of virgin coconut oil (VCO) on the epithelium thickness and the diameter of the seminiferous tubules induced by ethanol in mice (Mus musculus). This study used 20 male mice as the experimental animal which were divided into five groups with four mice in each group. Negative control (C-) was given 2% Tween and aquadest, while positive control (C+) was given 2% Tween and 33% ethanol. T1, T2, and T3 were respectively given 0.09, 0.19, and 0.37 mL/kg bw VCO and 33% ethanol (0.2 mL/kg bw). VCO was given orally for 39 days, and ethanol was given orally seven days later for 32 days. Ethanol was administered two hours after the VCO administration. Analysis of variance followed by Tukey’s range test on the epithelium thickness and the diameter of the seminiferous tubules showed significant differences (p <0.05) of C+ group from the other groups. Whereas, there was no significant difference (p >0.05) was found among C-, T1, T2 and T3 group. The result concluded that VCO could protect the testis of mice from the damage caused by ethanol.
Moringa oleifera leaf extract restored the diameter and epithelium thickness of the seminiferous tubules of rat (Rattus norvegicus) injected with gentamicin Meidy Mardatillah; Wurlina Wurlina; Ira Sari Yudaniayanti; Hardany Primarizky; Hani Plumeriastuti; Iwan Sahrial Hamid
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.15-21

Abstract

This research aims to determine the effect of Moringa oleifera leaf extract (MLE) on the diameter and epithelium thickness of the seminiferous tubules of rats induced with gentamicin. This study used 25 male rats (Rattus norvegicus) aged 8-12 weeks with 150-200 grams body weight. Negative control group (C-) was injected with aquadest and given 0.5% Na-CMC orally, positive control group (C+) was induced with gentamicin injection of 5 mg/kg BW/day and given 0.5% Na-CMC orally, while groups T1, T2, and T3 were induced with gentamicin at the same dose and treated orally with MLE of 200, 316, 500 mg/kg BW/day. Analysis of variance showed significant differences and further analysis using Duncan Multiple Range Test showed difference in each group. The results showed that treatment with MLE of 500 mg/kg BW/day improved the diameter and epithelium thickness of the seminiferous tubules of rats injected with gentamicin. It could be concluded that MLE restored the diameter and epithelium thickness of the seminiferous tubules of rats induced with gentamicin.
Identification of Ectoparasites in Pearl Catfish (Clarias gariepinus) One and Three Months Ages in Makalele Cultivation Tuban District, Tuban Regency Galuh Swa Ardhya; Azizatun Nimah; Hani Plumeriastuti; Setiawan Koesdarto; Budiarto Budiarto
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1001.861 KB) | DOI: 10.20473/jops.v6i2.34257

Abstract

ABSTRACT             The purpose of this study was to identify ectoparasites that attack pearl catfish and the incidence of ectoparasite infestation in 100 samples of pearl catfish aged one and three months. This research was carried out from January to March 2021 in Makalele's cultivation, Tuban's District, Tuban's Regency. The method used in this research is skin scraping and gill installation. Data analysis using Chi-Square Test with SPSS version 24.0 for windows. The results showed that there were 3 types of ectoparasites found, Trichodina nobilis, Trichodina pediculus, Chilodonella sp., Dactylogyrus sp. and the highest incidence of ectoparasites occurred in young fish compared to old fish with a percentage of 35% and 25%, respectively, and based on the chi-square test there was no significant difference (p️≥0.05). Keywords: Clarias gariepinus, infestation level, ectoparasites.
Effect of taurine on histopathological features of spermatogenesis in seminiferous tubules of mice (Mus musculus) induced by paraquat Yustisiane Ruth Rahadi; Tri Wahyu Suprayogi; Rahmi Sugihartuti; Kadek Rachmawati; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 2 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i2.2022.66-71

Abstract

This study aimed to determine the effect of taurine on the enhancement of the spermatogenetic process in male mice (Mus musculus) induced by paraquat (PQ). Twenty-five male mice (Mus musculus) aged 2-3 months with a bodyweight of around 35 grams were divided randomly into five groups. The K + and the treatment group (P1, P2, and P3) mice were induced using PQ. PQ was given intraperitoneally (IP) twice a week for 21 consecutive days at a dose of 30 mg/kg BW. Two hours after the administration of PQ, P1, P2, and P3 groups were given taurine at a dose of 250, 500, and 1000 mg/kg BW/day for three weeks (Heidari et al., 2019). K- group was given distilled water (IP) only. On day-29, mice were sacrificed for testicles histopathological preparations with hematoxylin-eosin staining. Results showed that the mice exposed to PQ only (the K+ group) had a reduced spermatogenesis score compared to those of the K- group (p <0.05).  Taurine treatment on PQ-exposed mice was followed by an increase spermatogenesis score. The optimal curative dose of taurine was 500 mg/kg (P2 group). However, a higher dose (1000 mg/kg BW) of taurine resulted in a decline in the spermatogenesis score than those of at the 500 mg/kg. It could be concluded that treatment with taurine could enhance the spermatogenetic process of male mice (Mus musculus) induced by PQ.
Determination of sexual maturity of Indonesian box turtle (Cuora amboinensis couro) based on straight carapace length Daniel Leonardo; Pudji Srianto; Iwan Sahrial Hamid; Boedi Setiawan; Djoko Legowo; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.115-122

Abstract

Predicting the turtle's reproductive status and sexual maturity is necessary for ex-situ breeding and conservation program. The histology of the seminiferous tubules of the Indonesian box turtle (Cuora amboinensis couro) at various ages has not been studied much. This study aims to develop equations to predict the sexual maturity of Indonesian box turtles based on straight carapace length (SCL). Six turtles with SCL sizes 10-21 cm were sacrificed and their testes were taken for morphometric measurements and histological preparations for Hematoxylin Eosin staining. Pearson’s correlation of SCL with testicular maturity parameters was analyzed, followed by Anova Regression. The results showed that there were differences in the morphometry and topographic anatomy of the Indonesian box turtle testes between SCL below 15 cm and above 15 cm. Six turtles with SCL size of 10-21 cm were sacrificed and their testes were taken for morphometric measurements and histological preparations for Hematoxylin Eosin staining. Pearson's correlation of SCL with testicular maturity parameters was analyzed, followed by Regression Anova. The results showed that there were differences in the morphometry and topographic anatomy of the Indonesian box turtle testes between SCL below 15 cm and above 15 cm.
Co-Authors AA Sudharmawan, AA Abdul Samik Adiana Mutamsari Witaningrum Adikara, Tatang Santanu Akbar Wijaya Putra Purnama Al arif, Mohammad Anam Al-Anshori, Akhmad Afifudin Aldin Akbar Rahmatullah Amaris, Aulia Puspa Anastasya, Jelita Andriani, Ratna Anggreani Desi Ramadhani Rahajeng Anindya, Afifatus Annisa Nur Fitria Rahma Annise Proboningrat Anwar Ma'ruf Arimbi Arimbi Arimbi Ayang Mahindra Ayu, Zerlinda Dyah Azizatun Nimah Bambang Prajogo Eko Wardoyo Batubara, Agung Setia Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan BUDI UTOMO Budiarto Budiarto Cahyono, Shahani Azpriyanne Chairul A. Nidom Chairul Anwar Christian Marco Hadi Nugroho Christy, Rachella Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Daniel Leonardo Darsono, Roesno Dayanti, Efin Windi Dewita Dewita Dhesy Kartikasari Didik Handijatno Djoko Legowo Dwi Puspa Sukma Viranda Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati ERMA SAFITRI Erma Safitri Estoepangestie, Agnes Theresia Soelih Fedik Abdul Rantam Fierda Kabayo Fiqih, Adam Fahmi Galaxy Guardian Galuh Swa Ardhya Ganeswara Muharam Hazmi Rezady Habibi, Muhammad Afif Hana Eliyani Hariadi, Mas'ud Hendarti, Gracia Angelina Heni Puspitasari Hermin Ratnani Hernanda, Ary Setya Hidajati, Nove Hidanah, Sri Imam Mustofa IMAM MUSTOFA Ira Sari Yudaniayanti Irfannur, Irfannur Ismi, Alif Noviana Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Jola Rahmahani Kadek Rachmawati Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Kinasih, Kurnia Nisa Kusnoto Kusnoto Kusnoto Lake, Werenfridus Kono Legowo, Djoko Lilik Maslachah Lita Rakhma Yustinasari Lucia Tri Suwanti, Lucia Tri Lukman, Epy Muhammad Luqman, Epy Muhammad Luthfiatu Kanina Mabluhatus Solehah Mafruchati, Maslichah Meidy Mardatillah Mirni Lamid Mochamad Lazuardi Mohammad Sukmanadi Muchammad Yunus Mufa, Ramy Inas Mahirah Mufasirin Muhammad Thohawi Elziyad Purnama Muliari Muliari Mustika, Yolla Rona Mustofa Helmi Effendi Nidom, Chairul Anwar Niluh Selly Frantika Nisa, Hanifah Khairun Nove Hidajati Novitasari, Seline Putri Nursadida, Milla Nusdianto Triakoso Nusdianto Triakoso Palupi, Tantri Dyah Widhi Paramita, Chici Ayu Paraswita Eindah Fitri Poedji Hastutiek Prameswari, Febriana Hawa Prastiya, Ragil Angga Prestalia Dwi Rachmawati Primarizky, Hardany Pudji Srianto Putri, Anandia Nafisah Rachmani, Sartika Dewi Rahaju Ernawati Rahmaniabdi, El Roy Achmad Rahmi Sugihartuti Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Retno Widyowati Retno Yuli Rimayanti Rimayanti Rina Vitriasari Ritonga, Mudhita Zikkrullah Rochmah Kurnijasanti Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Dian Ayu Kartika Sarmanu, Sarmanu Sarudji, Suryanie Setiawan Koesdarto Sherina Lashita Candrakirana Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Sofyan, Miayu Soneta Solikhah, Aviatus Sri Chusniati Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Suhud, Mohamad Kharis Sunarso, Agus Supranianondo, Koesnoto Suzanita Utama Tantri Nawangwulan Tatik Hernawati Tjuk Imam Restiadi Tony Hartono Tri Wahyu Suprayogi Trilas Sardjito Tyasningsih, Wiwiek Vishantheni S Ravi Weliyani, Trima Wibawati, Prima Ayu Widisuputri, Ni Komang Aprilina Widiyatno, Thomas Valentinus Widjiati w Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Xin, Irene Teh Kai Yeni Dhamayanti Yonatan Dimascahyo Budianto Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Yunus, Muchammad Yusrizal Akmal Yustinasari, Lita Rakhma Yustisiane Ruth Rahadi Zahro, Vida Seanita Zhaza Afililla Zulkifli, Arief Hidayat