Claim Missing Document
Check
Articles

Porang (Amorphophallus oncophyllus) tuber extract improved the histopathological features of diabetic rat (Rattus norvegicus) testicles Dwi Puspa Sukma Viranda; Rochmah Kurnijasanti; Kadek Rachmawati; Iwan Sahrial Hamid; Pudji Srianto; Hani Plumeriastuti; Lita Rakhma Yustinasari
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.47-57

Abstract

This study aims to determine the effect of porang (Amorphophallus oncophyllus) tuber extract on the histopathological features of the testicle of alloxan induced rats (Rattus norvegicus). Thirty male rats were randomly divided into six groups. Rats in group C- were injected with distilled water, while rats in groups C+, T0, T1, T2, and T3 were injected with 120 mg/kg bw alloxan. Blood glucose was measured three days after alloxan injection. Rats in groups C- and C+ were then administered with 1% Na-CMC, whereas rats in groups T0, T1, T2, and T3 were then administered with 45mg/kg bw metformin, and 100, 200, and 400 mg/kg bw of porang tuber extract, respectively. All solution were given orally once every day for 14 days. Spermatogenic activity was assessed using the Johnsen scoring system and analyzed by Kruskal-Wallis, followed by the Mann-Whitney test. The diameter and epithelial thickness of the seminiferous tubule were measured using image raster software and analyzed using Anova followed by Duncan's test. The results showed that spermatogenesis score, diameter, and epithelial thickness of seminiferous tubule of group C+ were smaller (p <0.05) than group C-. Spermatogenesis scores of groups T0, T1, T2, and T3 were higher (p <0.05) than group C+. Seminiferous tubule diameter and epithelial thickness in groups T0, T1, and T2 were greater (p <0.05) than group C+. It could be concluded that porang tuber extract at a dose of 200 mg/kg bw improved the spermatogenesis score, diameter seminiferous tubule, and thickness of the epithelium of diabetic rats.
The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

Abstract

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Nephroprotective Effect of Dayak Onion (Eleutherine palmifolia) Against Monosodium Glutamate-Induced Renal Toxicity in Mice (Mus musculus) Anggreani Desi Ramadhani Rahajeng; Aldin Akbar Rahmatullah; Cindy Ercha Aulia Putri; Rahmi Sugihartuti; Endang Suprihati; Hani Plumeriastuti; Mohammad Sukmanadi; Iwan Sahrial Hamid
Journal of Applied Veterinary Science And Technology Vol. 5 No. 2 (2024): October 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I2.2024.129-134

Abstract

Background: The toxicological impacts of monosodium glutamate (MSG), a commonly used food additive, present a significant public health concern. Excessive MSG consumption will increase radical oxidative species, inducing a stress oxidative condition that ultimately results in kidney damage. Purpose: This study aimed to explore the nephroprotective effects of ethanol extracts from Dayak onion (Eleutherine palmifolia) against MSG-induced renal toxicity in mice (Mus musculus). Method: Twenty-five 11 week-old male mice, weighing approximately 20 g each, were divided into five research groups. Group C- received only CMC-Na 0.5%, Group C+ received 4000 mg/kg BW MSG, while Groups T1, T2, and T3 were administered 4000 mg/kg BW MSG along with Dayak onion extract at doses of 30 mg/kg BW, 60 mg/kg BW, and 120 mg/kg BW respectively. All treatments were carried out orally for 52 days. The mice were euthanized by cervical dislocation, and their kidneys were extracted for the examination of any histopathological changes. The data underwent analysis utilizing the Kruskal-Wallis test, followed by the Mann-Whitney test. Results: The results as indicated by the histopathological evaluation of the mice’s kidneys revealed significant improvements in the histo-architecture of the kidneys. Supplementation of Dayak onion extract in mice induced with MSG decreased the degeneration and necrosis of the tubule epithelium, and it also repaired the glomerular necrosis. Conclusion: Oral administration of Dayak onion extract to mice exposed to monosodium glutamate demonstrated a notable reduction in kidney damage and helped maintain renal health significantly.
Pengaruh Pemberian Nanocurcumin (Curcuma longa) Terhadap Gambaran Histopatologis Pulmo Tikus (Rattus norvegicus) Yang Dipapar Carbon Black Rahmaniabdi, El Roy Achmad; Mustofa, Imam; Hestianah, Eka Pramyrtha; Widjiati, Widjiati; Kurnijasanti, Rochmah; Plumeriastuti, Hani
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.38

Abstract

Background Exposure to carbon black can induce respiratory toxicity, leading to tissue remodeling, inflammation, and fibrosis. Curcumin, a compound from Curcuma longa, has been shown to exert protective effects on lung tissue by inhibiting cell proliferation and reducing alveolar epithelial thickening and inflammatory responses. Objective This study aimed to evaluate the effect of nanocurcumin administration on alveolar septal thickness and fibrosis in the lungs of rats (Rattus norvegicus) exposed to carbon black. Methods Thirty female rats were divided into five groups: the negative control group (K-) received distilled water without carbon black exposure; the positive control group (K+) received distilled water and was exposed to carbon black at a dose of 1064 mg/m³ for 6 hours; and treatment groups P1, P2, and P3 received nanocurcumin at doses of 50, 100, and 200 mg/kg body weight, respectively, along with carbon black exposure at a dose of 1064 mg/m³ for 6 hours/day over 30 days. Results Alveolar septal thickness and fibrosis in the P1, P2, and P3 groups showed significant reductions (P<0.05) compared to the K+ group. Notably, groups P2 and P3 exhibited similar alveolar septal thickness to the K- group, with statistically lower (P<0.05) fibrosis levels than P1 but not significantly different from K- (P>0.05). Conclusion Nanocurcumin administration demonstrates potential in mitigating alveolar septal thickening and fibrosis in rat lungs subjected to carbon black exposure, suggesting a protective role against pulmonary damage from particulate matter.
Detection of Nematode on Feces of Snail at Malang, East Java Niluh Selly Frantika; Kusnoto; Lucia Tri Suwanti; Imam Mustofa; Endang Suprihati; Hani Plumeriastuti
Journal of Parasite Science Vol. 9 No. 1 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i1.65007

Abstract

Acathina fulica is a terrestrial mollusk that often invades terrestrial vegetation that has tropical and subtropical climates. Acathina fulica has been widely reported in environments as diverse as urban areas, forests, capoeiras, caatings, plantations, decomposing materials, savannahs and vegetable gardens. Soil is source of food and a defense strategy for A. fulica, therefore it has an important role for them. Therefore, A. fulica may be host several species of nematodes. Microscopic examination of snails A. fulica fecal samples in Cemorokandang Village, Malang City using the native method aimed to detect Rhabditis spp. The results showed that 14 (12.5%) out of 112 samples contained Rhabditis spp. The genus rhabditis belongs to the Rhabditidae family and is one of the free-living nematodes. This species has long been known and of concern in the veterinary world. The genus rhabditis has been reported to have infected humans and have been isolated from urine samples, vaginal swab, and human stool. The research was conducted in the dry season with low rainfall. Nematode of the genus Rhabditis spp. can be naturally found in humid places and decaying organic matter.
Toxoplasma Oocyst Contamination in Water Sources in Goat-Sheep Farming In Sidoarjo District Mufa, Ramy Inas Mahirah; Eka Pramyrtha Hestianah; Lucia Tri Suwanti; Hani Plumeriastuti; Widya Paramita Lokapirnasari; Tony Hartono; Rina Vitriasari; Retno Yuli; Galaxy Guardian; Heni Puspitasari; Mufasirin
Journal of Parasite Science Vol. 9 No. 1 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i1.67669

Abstract

Toxoplasmosis is one of the zoonotic diseases caused by the protozoa Toxoplasma gondii. The disease infects warm-blooded animals including humans. The purpose of this study was to determine the contamination of Toxoplasma oocysts in water sources in goat-sheep farms in Sidoarjo Regency. A total of 63 water samples were used for the study. Water samples were taken from 5 sub-districts that have many goat-sheep farms, each sub-district has 2 villages, with each village having 6-7 samples. The examination used the floating method and was examined under a microscope with a magnification of 400x. The results of the examination found 21 samples of Toxoplasma gondii oocysts or 33.33% (21/63). The morphology of T.gondii oocysts is round, ovoid or ellipsoid with sizes varying between 8.02 - 12.72 micrometers. Preventive measures are needed by treating water before giving it to livestock.
Prophylaxic study of Combination Ethyl Acetate Fraction of Andrographis paniculata Ness. and Dihidroartemisinin-Piperaquine (DHP) in Malarial Infected Mice Nisa, Hanifah Khairun; Solikhah, Aviatus; Plumeriastuti, Hani
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.203-211

Abstract

The resistance to some antimalarial drugs has had a major impact on the drug selection for malaria prophylaxis. According to previous research, the combination of Andrographis paniculata and Dihydroartemisinin-piperaquine (DHP) can prevent artemisinin resistance, reduce the adverse effects and is highly effective as an antimalarial. This study aims to determine the malaria prophylactic activity and survival time of the combination of A. paniculata ethyl acetate fraction with DHP on P. berghei infected mice. An in vivo malaria prophylaxis study was conducted based on Peters’ prophylactic test method. The combination of A. paniculata ethyl acetate fraction at a dose of 100 mg andrographolide/kg BW was administered twice a day per oral for four days and continued for another day after infection, meanwhile DHP at a dose 1,6 mg/kg BW was administered once a day per oral for three days. These results show that the combination of A. paniculata ethyl acetate fraction with DHP can inhibit 54,35% of the malaria parasite on day 4 and 44,98% on day 5, which was less active than the monotherapy group of A. paniculata ethyl acetate fraction or DHP alone. Also, this combination can increase the survival time of the mice to 6 days.
Comparative appendicular osteology of Tor spp. (Cyprinidae) in Indonesia Akmal, Yusrizal; Yunus, Muchammad; Plumeriastuti, Hani; Dhamayanti, Yeni; Irfannur, Irfannur; Zulkifli, Arief Hidayat; Muliari, Muliari; Batubara, Agung Setia
Depik Vol 13, No 1 (2024): APRIL 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.1.28247

Abstract

Tor tambroides, T. tambra, T. douronensis, and T. soro are native fish species to Indonesia with their natural habitat generally being upstream of rivers in hilly areas with clear water and strong currents. Information of the structure and function of appendicular osteology is necessary to understand how fish accelerate and maintain body balance in the water. This study used 5 samples per fish species obtained from Tor fishermen around the habitat of these fish. The results of the comparison showed morphological differences between the appendicular structure of Tor species, consisting structure of the pinnae pectoralis, pinnae pelvicalis, pinnae dorsalis, pinnae analis, and pinnae caudalis. The highest variation was found in the pinnae caudalis. The pointed shape of the pinnae caudalis with long fin rays indicates that the Tor species is capable to swim at a high speeds.Keywords:ossa appendiculareTor douronensisTor tambraTor tambroidesTor soro
Diversity And Characteristics of Bladder Stones in Green Iguanas (Iguana iguana): A Case Report Hendarti, Gracia Angelina; Sofyan, Miayu Soneta; Sari, Dian Ayu Kartika; Plumeriastuti, Hani; Proboningrat, Annise; Dhamayanti, Yeni; Soeharsono, Soeharsono; Lukman, Epy Muhammad; Palupi, Tantri Dyah Widhi; Hernanda, Ary Setya; Ritonga, Mudhita Zikkrullah
Jurnal Medika Veterinaria Vol 19, No 1 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i1.45206

Abstract

Bladder stones represent a significant and increasingly common health issue in captive green iguanas (Iguana iguana), often resulting from improper husbandry practices. This case report examines the diversity and characteristics of bladder stones in five green iguanas (three females, two males) presenting with abdominal enlargement and decreased appetite. Surgical removal (cystotomy) was performed, followed by detailed morphometric and chemical analyses of the extracted stones. The stones varied in size (15.3655.01 g), shape, and color (white, cream, and dark brown), yet chemical composition was consistently dominated by calcium oxalate (5260%), tricalcium phosphate (2328%), and uric acid (1012%). Morphometric analysis showed low variation (50%) across measured parameters, indicating homogeneity among the samples. Notably, lighter iguanas tended to harbor heavier stones, suggesting a correlation with disease progression rather than sex or stone color. These findings reinforce the role of dietary imbalances, dehydration, and the anatomical peculiarities of the iguana urinary system in stone formation. Early detection, appropriate diet, and optimal hydration are critical to prevent urolithiasis. This report contributes to the limited data on iguana urolithiasis in Indonesia and highlights the need for broader studies to explore preventive strategies.
The effect of binder mycotoxins on the histopathology of broiler kidneys exposed to a combination of mycotoxins Anastasya, Jelita; Mirni Lamid; Poedji Hastutiek; Erma Safitri; Mohammad Anam Al-Arif; Hani Plumeriastuti
Jurnal Agro Veteriner Vol. 8 No. 2 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i2.74036

Abstract

This study was made to know the histopathological changes of kidney in broiler chicken fed by mix mycotoxin (aflatoxin B1 and ochratoxin A) contaminated feed. A total of 20 chickens aged DOC to 35 days was divided into 4 groups (K-, K+, P1 and P2) each group consisted of 5 chickens. Group K- as a control without the addition of the mix mycotoxin to the fed. Group K+ feed was added with 0.1 mg/kg of aflatoxin B1 and 0.1 mg/kg of ochratoxin A. Group P1 and P2 feed were added mix mycotoxin with the same dose with addition of 1.1 g/kg mycotoxin binder in P1 group and 1.6 g/kg of mycotoxin binder in P2 group. Treatment of contaminated feed has been given for 28 days from day 8 until day 35. At the end of the treatment period (day 35), chickens were euthanized, and histopathological examination was carried out. Kruskal-Wallis test showed a significant difference (p<0.05) on the mean rank of the kidney cell necrosis, cell degeneration, inflammatory cell infiltration, and haemorrhage. The conclusion of this study is that the administration of mycotoxin binder has an effect in preventing and reducing the necrosis, degeneration, inflammatory cell infiltration, and kidney haemorrhage.
Co-Authors AA Sudharmawan, AA Abdul Samik, Abdul Adiana Mutamsari Witaningrum Adikara, Tatang Santanu Agnes Theresia Soelih Estoepangestie Agung Setia Batubara Agung Setia Batubara Akbar Wijaya Putra Purnama Al arif, Mohammad Anam Al-Anshori, Akhmad Afifudin Aldin Akbar Rahmatullah Amaris, Aulia Puspa Anastasya, Jelita Andriani, Ratna Anggreani Desi Ramadhani Rahajeng Anindya, Afifatus Annisa Nur Fitria Rahma Annise Proboningrat Anwar Ma'ruf Arief Hidayat Zulkifli Arimbi Arimbi Arimbi Ayang Mahindra Ayu, Zerlinda Dyah Azizatun Nimah Bachtiar, Harris Yudhanta Bambang Prajogo Eko Wardojo Batubara, Agung Setia Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan Budi Utomo BUDI UTOMO Budiarto Budiarto Cahyono, Shahani Azpriyanne Chairul A. Nidom Chairul Anwar Christian Marco Hadi Nugroho Christy, Rachella Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Daniel Leonardo Darsono, Roesno Dayanti, Efin Windi Dewita Dewita Dhesy Kartikasari Didik Handijatno Djoko Legowo Dwi Puspa Sukma Viranda Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Epy Muhammad Luqman Erma Safitri ERMA SAFITRI Fedik Abdul Rantam Fiqih, Adam Fahmi Fitri, Paraswita Eindah Galaxy Guardian Galuh Swa Ardhya Habibi, Muhammad Afif Hana Eliyani Hendarti, Gracia Angelina Heni Puspitasari Hermin Ratnani Hernanda, Ary Setya Hidanah, Sri Hidayatik, Nanik IMAM MUSTOFA Imam Mustofa Ira Sari Yudaniayanti Irfannur Irfannur Irfannur Irfannur Irfannur, Irfannur Ismi, Alif Noviana Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Kabayo, Fierda Kadek Rachmawati Kartikasari, Dhesy Khairullah, Aswin Rafif Kinasih, Kurnia Nisa Kuncorojakti, Suryo Kusnoto Kusnoto Kusnoto Lake, Werenfridus Kono Legowo, Djoko Lilik Maslachah Lita Rakhma Yustinasari Lucia Tri Suwanti, Lucia Tri Lukman, Epy Muhammad Luthfiatu Kanina Mabluhatus Solehah Mafruchati, Maslichah Mardatillah, Meidy Mas’ud Hariadi Mirni Lamid Mochamad Lazuardi Mohammad Sukmanadi Muchammad Yunus Muchammad Yunus Mufa, Ramy Inas Mahirah Mufasirin Muhammad Thohawi Elziyad Purnama Muliari Muliari Muliari Muliari Mustika, Yolla Rona Mustofa Helmi Effendi Nawangwulan, Tantri Nidom, Chairul Anwar Niluh Selly Frantika Nisa, Hanifah Khairun Nove Hidajati Novitasari, Seline Putri Nursadida, Milla Nusdianto Triakoso Palupi, Tantri Dyah Widhi Paramita, Chici Ayu Poedji Hastutiek Prameswari, Febriana Hawa Prastiya, Ragil Angga Prestalia Dwi Rachmawati Primarizky, Hardany Pudji Srianto Putri, Anandia Nafisah Putri, Mirtanti Kiswandi Rachmani, Sartika Dewi Rahadi, Yustisiane Ruth Rahaju Ernawati Rahmahani, Jola Rahmaniabdi, El Roy Achmad Rahmi Sugihartuti Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Retno Widyowati Retno Yuli Rezady, Ganeswara Muharam Hazmi Rimayanti Rimayanti Rina Vitriasari Ritonga, Mudhita Zikkrullah Rochmah Kurnijasanti S Ravi, Vishantheni Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Dian Ayu Kartika Sarmanu, Sarmanu Setiawan Koesdarto Sherina Lashita Candrakirana Soeharsono Soeharsono Sofyan, Miayu Soneta Solikhah, Aviatus Sri Mulyati Sri Mulyati Sri Pantja Madyawati Suherni Susilowati Suhud, Mohamad Kharis Sukmanadi, Mohammad Sunarso, Agus Supranianondo, Koesnoto Suryanie Sarudji Suzanita Utama Tatik Hernawati Tjuk Imam Restiadi Tony Hartono Tri Wahyu Suprayogi Trilas Sardjito Tyasningsih, Wiwiek Weliyani, Trima Wibawati, Prima Ayu Widisuputri, Ni Komang Aprilina Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Xin, Irene Teh Kai Yeni Dhamayanti Yonatan Dimascahyo Budianto Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Yunus, Muchammad Yusrizal Akmal Zahro, Vida Seanita Zhaza Afililla Zulkifli, Arief Hidayat