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Prophylaxic study of Combination Ethyl Acetate Fraction of Andrographis paniculata Ness. and Dihidroartemisinin-Piperaquine (DHP) in Malarial Infected Mice Nisa, Hanifah Khairun; Solikhah, Aviatus; Plumeriastuti, Hani
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.203-211

Abstract

The resistance to some antimalarial drugs has had a major impact on the drug selection for malaria prophylaxis. According to previous research, the combination of Andrographis paniculata and Dihydroartemisinin-piperaquine (DHP) can prevent artemisinin resistance, reduce the adverse effects and is highly effective as an antimalarial. This study aims to determine the malaria prophylactic activity and survival time of the combination of A. paniculata ethyl acetate fraction with DHP on P. berghei infected mice. An in vivo malaria prophylaxis study was conducted based on Peters’ prophylactic test method. The combination of A. paniculata ethyl acetate fraction at a dose of 100 mg andrographolide/kg BW was administered twice a day per oral for four days and continued for another day after infection, meanwhile DHP at a dose 1,6 mg/kg BW was administered once a day per oral for three days. These results show that the combination of A. paniculata ethyl acetate fraction with DHP can inhibit 54,35% of the malaria parasite on day 4 and 44,98% on day 5, which was less active than the monotherapy group of A. paniculata ethyl acetate fraction or DHP alone. Also, this combination can increase the survival time of the mice to 6 days.
Comparative appendicular osteology of Tor spp. (Cyprinidae) in Indonesia Akmal, Yusrizal; Yunus, Muchammad; Plumeriastuti, Hani; Dhamayanti, Yeni; Irfannur, Irfannur; Zulkifli, Arief Hidayat; Muliari, Muliari; Batubara, Agung Setia
Depik Vol 13, No 1 (2024): APRIL 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.1.28247

Abstract

Tor tambroides, T. tambra, T. douronensis, and T. soro are native fish species to Indonesia with their natural habitat generally being upstream of rivers in hilly areas with clear water and strong currents. Information of the structure and function of appendicular osteology is necessary to understand how fish accelerate and maintain body balance in the water. This study used 5 samples per fish species obtained from Tor fishermen around the habitat of these fish. The results of the comparison showed morphological differences between the appendicular structure of Tor species, consisting structure of the pinnae pectoralis, pinnae pelvicalis, pinnae dorsalis, pinnae analis, and pinnae caudalis. The highest variation was found in the pinnae caudalis. The pointed shape of the pinnae caudalis with long fin rays indicates that the Tor species is capable to swim at a high speeds.Keywords:ossa appendiculareTor douronensisTor tambraTor tambroidesTor soro
Comparative appendicular osteology of Tor spp. (Cyprinidae) in Indonesia Akmal, Yusrizal; Yunus, Muchammad; Plumeriastuti, Hani; Dhamayanti, Yeni; Irfannur, Irfannur; Zulkifli, Arief Hidayat; Muliari, Muliari; Batubara, Agung Setia
Depik Vol 13, No 1 (2024): APRIL 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.1.28247

Abstract

Tor tambroides, T. tambra, T. douronensis, and T. soro are native fish species to Indonesia with their natural habitat generally being upstream of rivers in hilly areas with clear water and strong currents. Information of the structure and function of appendicular osteology is necessary to understand how fish accelerate and maintain body balance in the water. This study used 5 samples per fish species obtained from Tor fishermen around the habitat of these fish. The results of the comparison showed morphological differences between the appendicular structure of Tor species, consisting structure of the pinnae pectoralis, pinnae pelvicalis, pinnae dorsalis, pinnae analis, and pinnae caudalis. The highest variation was found in the pinnae caudalis. The pointed shape of the pinnae caudalis with long fin rays indicates that the Tor species is capable to swim at a high speeds.Keywords:ossa appendiculareTor douronensisTor tambraTor tambroidesTor soro
Diversity And Characteristics of Bladder Stones in Green Iguanas (Iguana iguana): A Case Report Hendarti, Gracia Angelina; Sofyan, Miayu Soneta; Sari, Dian Ayu Kartika; Plumeriastuti, Hani; Proboningrat, Annise; Dhamayanti, Yeni; Soeharsono, Soeharsono; Lukman, Epy Muhammad; Palupi, Tantri Dyah Widhi; Hernanda, Ary Setya; Ritonga, Mudhita Zikkrullah
Jurnal Medika Veterinaria Vol 19, No 1 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i1.45206

Abstract

Bladder stones represent a significant and increasingly common health issue in captive green iguanas (Iguana iguana), often resulting from improper husbandry practices. This case report examines the diversity and characteristics of bladder stones in five green iguanas (three females, two males) presenting with abdominal enlargement and decreased appetite. Surgical removal (cystotomy) was performed, followed by detailed morphometric and chemical analyses of the extracted stones. The stones varied in size (15.3655.01 g), shape, and color (white, cream, and dark brown), yet chemical composition was consistently dominated by calcium oxalate (5260%), tricalcium phosphate (2328%), and uric acid (1012%). Morphometric analysis showed low variation (50%) across measured parameters, indicating homogeneity among the samples. Notably, lighter iguanas tended to harbor heavier stones, suggesting a correlation with disease progression rather than sex or stone color. These findings reinforce the role of dietary imbalances, dehydration, and the anatomical peculiarities of the iguana urinary system in stone formation. Early detection, appropriate diet, and optimal hydration are critical to prevent urolithiasis. This report contributes to the limited data on iguana urolithiasis in Indonesia and highlights the need for broader studies to explore preventive strategies.
The effect of binder mycotoxins on the histopathology of broiler kidneys exposed to a combination of mycotoxins Anastasya, Jelita; Mirni Lamid; Poedji Hastutiek; Erma Safitri; Mohammad Anam Al-Arif; Hani Plumeriastuti
Jurnal Agro Veteriner Vol. 8 No. 2 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i2.74036

Abstract

This study was made to know the histopathological changes of kidney in broiler chicken fed by mix mycotoxin (aflatoxin B1 and ochratoxin A) contaminated feed. A total of 20 chickens aged DOC to 35 days was divided into 4 groups (K-, K+, P1 and P2) each group consisted of 5 chickens. Group K- as a control without the addition of the mix mycotoxin to the fed. Group K+ feed was added with 0.1 mg/kg of aflatoxin B1 and 0.1 mg/kg of ochratoxin A. Group P1 and P2 feed were added mix mycotoxin with the same dose with addition of 1.1 g/kg mycotoxin binder in P1 group and 1.6 g/kg of mycotoxin binder in P2 group. Treatment of contaminated feed has been given for 28 days from day 8 until day 35. At the end of the treatment period (day 35), chickens were euthanized, and histopathological examination was carried out. Kruskal-Wallis test showed a significant difference (p<0.05) on the mean rank of the kidney cell necrosis, cell degeneration, inflammatory cell infiltration, and haemorrhage. The conclusion of this study is that the administration of mycotoxin binder has an effect in preventing and reducing the necrosis, degeneration, inflammatory cell infiltration, and kidney haemorrhage.
Efektivitas Kitosan dari Limbah Kulit Udang terhadap Angiogenesis dalam Penyembuhan Luka Eksisi pada Tikus Putih (Rattus norvegicus) Jantan Dayanti, Efin Windi; Arimbi, Arimbi; Yunita, Maya Nurwartanti; Plumeriastuti, Hani; Purnama, Muhammad Thohawi Elziyad; Wibawati, Prima Ayu
Media Kedokteran Hewan Vol. 32 No. 2 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i2.2021.60-69

Abstract

Luka eksisi adalah luka yang disebabkan akibat terpotongnya jaringan oleh benda yang tajam (Partogi, 2008). Kitosan mampu sebagai zat dalam penyembuhan luka salah satunya dalam proses angiogenesis. Tujuan penelitian ini yaitu untuk mengetahui efektivitas salep kitosan dari limbah kulit udang terhadap angiogenesis dalam penyembuhan luka eksisi pada tikus putih jantan. Sebanyak dua puluh ekor tikus putih (Rattus norvegicus) jantan dibagi ke dalam lima kelompok perlakuan yaitu K+ luka eksisi dengan pemberian povidone iodine, K- luka eksisi dengan pemberian salep tanpa kitosan, P1 luka eksisi dengan pemberian salep kitosan 1,5%, P2 luka eksisi dengan pemberian salep 2,5% dan P3 luka eksisi dengan pemberian salep kitosan. Terapi diberikan sehari sekali selama tujuh hari. Hasil data rata-rata jumlah pembuluh darah baru dianalisis dengan uji Anova yang menunjukkan adanya perbedaan yang signifikan (p<0,05) dan dilanjutkan dengan uji LSD (Least Significant Differences). Kelompok P1 dan P2 tidak memiliki perbedaan yang nyata, tetapi berbeda nyata dengan kelompok K+, K- dan P3. Kesimpulan dari penelitian ini kitosan efektif dalam meningkatkan jumlah pembuluh darah baru pada proses penyembuhan luka eksisi.
Antimicrobial effect of Methanol and Ethanol Extracts of Kembang Bulan (Tithonia diversifolia) Leaves against Staphylococcus aureus Plumeriastuti, Hani; Habibi, Muhammad Afif; Tehupuring, Benjamin Christoffel; Effendi, Mustofa Helmi; Estoepangestie, Agnes Theresia Soelih; Sarudji, Suryanie; Witaningrum, Adiana Mutamsari
Media Kedokteran Hewan Vol. 32 No. 2 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i2.2021.97-104

Abstract

The purpose of this study was to determine how Staphylococcus aureus responded to Kembang Bulan (Tithonia diversifolia) leaf extract's antibacterial properties. Eight S. aureus bacterial isolates from three Surabaya dairy farms were used in this study. Tithonia diversifolia leaf extract at concentrations of 10%, 5%, 2.5%, 1.25%, and 0% were employed in the study's samples. The antibacterial activity of Tithonia diversifolia leaf extract was assessed in this work using the disk diffusion method and an inhibition zone estimated in millimeters. Based on the findings of this study, it was determined that Tithonia diversifolia leaf extract at 5% and 10% concentrations had 100% antibacterial activity against S. aureus in eight samples. As with S. aureus, Tithonia diversifolia leaf extract at concentrations of 0%, 1.25%, and 2.5% did not exhibit any antibacterial action.
Cytotoxicity of Kembang Bulan (Tithonia diversifolia ) Ethanolic Leaf Extract on Rabbit Limbal Mesenchymal Stem Cells Plumeriastuti, Hani; Zahro, Vida Seanita; Triakoso, Nusdianto; Rantam, Fedik Abdul; Hamid, Iwan Sahrial; Effendi, Mustofa Helmi; Proboningrat, Annise
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.233-239

Abstract

Kembang Bulan (Tithonia diversifolia ) is known as an herbal plant that has many useful properties to cure disease, such as diabetes, malaria, and other infections. Instead of much usefulness, T. diversifolia has main biological properties that may induce toxicity in this plant known as sesquiterpene lactones. The aim of this study was to identify the cytotoxicity effect of T. diversifolia leaf extract on limbal mesenchymal stem cells of rabbits using the MTT assay. T. diversifolia leaf extract was divided into 4 concentrations (0.5%, 0.25%, 0.125%, and 0.0625%) and given to limbal mesenchymal stem cells of rabbits in 96-well microplates, which would be incubated for 24 hours. The cytotoxicity result was obtained using the MTT assay method. Every well would be observed to see the cell life level of each concentration. The optical density absorbance was calculated using a microplate reader. T. diversifolia leaf extract with a concentration of 0.0625% has the biggest viability with a level of existence of limbal mesenchymal stem cells of 50%, and the other T. diversifolia leaf extract concentrations of 0.5%, 0.25%, and 0.125% have less than 50% level of existence of limbal mesenchymal stem cells.
Co-Authors AA Sudharmawan, AA Abdul Samik Adiana Mutamsari Witaningrum Adikara, Tatang Santanu Akbar Wijaya Putra Purnama Al arif, Mohammad Anam Al-Anshori, Akhmad Afifudin Aldin Akbar Rahmatullah Amaris, Aulia Puspa Anastasya, Jelita Andriani, Ratna Anggreani Desi Ramadhani Rahajeng Anindya, Afifatus Annisa Nur Fitria Rahma Annise Proboningrat Anwar Ma'ruf Arimbi Arimbi Arimbi Ayang Mahindra Ayu, Zerlinda Dyah Azizatun Nimah Bambang Prajogo Eko Wardoyo Batubara, Agung Setia Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan BUDI UTOMO Budiarto Budiarto Cahyono, Shahani Azpriyanne Chairul A. Nidom Chairul Anwar Christian Marco Hadi Nugroho Christy, Rachella Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Daniel Leonardo Darsono, Roesno Dayanti, Efin Windi Dewita Dewita Dhesy Kartikasari Didik Handijatno Djoko Legowo Dwi Puspa Sukma Viranda Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Erma Safitri ERMA SAFITRI Estoepangestie, Agnes Theresia Soelih Fedik Abdul Rantam Fierda Kabayo Fiqih, Adam Fahmi Galaxy Guardian Galuh Swa Ardhya Ganeswara Muharam Hazmi Rezady Habibi, Muhammad Afif Hana Eliyani Hariadi, Mas'ud Hendarti, Gracia Angelina Heni Puspitasari Hermin Ratnani Hernanda, Ary Setya Hidajati, Nove Hidanah, Sri Imam Mustofa IMAM MUSTOFA Ira Sari Yudaniayanti Irfannur, Irfannur Ismi, Alif Noviana Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Jola Rahmahani Kadek Rachmawati Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Kinasih, Kurnia Nisa Kusnoto Kusnoto Kusnoto Lake, Werenfridus Kono Legowo, Djoko Lilik Maslachah Lita Rakhma Yustinasari Lucia Tri Suwanti, Lucia Tri Lukman, Epy Muhammad Luqman, Epy Muhammad Luthfiatu Kanina Mabluhatus Solehah Mafruchati, Maslichah Meidy Mardatillah Mirni Lamid Mochamad Lazuardi Mohammad Sukmanadi Muchammad Yunus Mufa, Ramy Inas Mahirah Mufasirin Muhammad Thohawi Elziyad Purnama Muliari Muliari Mustika, Yolla Rona Mustofa Helmi Effendi Nidom, Chairul Anwar Niluh Selly Frantika Nisa, Hanifah Khairun Nove Hidajati Novitasari, Seline Putri Nursadida, Milla Nusdianto Triakoso Nusdianto Triakoso Palupi, Tantri Dyah Widhi Paramita, Chici Ayu Paraswita Eindah Fitri Poedji Hastutiek Prameswari, Febriana Hawa Prastiya, Ragil Angga Prestalia Dwi Rachmawati Primarizky, Hardany Pudji Srianto Putri, Anandia Nafisah Rachmani, Sartika Dewi Rahaju Ernawati Rahmaniabdi, El Roy Achmad Rahmi Sugihartuti Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Retno Widyowati Retno Yuli Rimayanti Rimayanti Rina Vitriasari Ritonga, Mudhita Zikkrullah Rochmah Kurnijasanti Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Dian Ayu Kartika Sarmanu, Sarmanu Sarudji, Suryanie Setiawan Koesdarto Sherina Lashita Candrakirana Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Sofyan, Miayu Soneta Solikhah, Aviatus Sri Chusniati Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Suhud, Mohamad Kharis Sunarso, Agus Supranianondo, Koesnoto Suzanita Utama Tantri Nawangwulan Tatik Hernawati Tjuk Imam Restiadi Tony Hartono Tri Wahyu Suprayogi Trilas Sardjito Tyasningsih, Wiwiek Vishantheni S Ravi Weliyani, Trima Wibawati, Prima Ayu Widisuputri, Ni Komang Aprilina Widiyatno, Thomas Valentinus Widjiati w Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Xin, Irene Teh Kai Yeni Dhamayanti Yonatan Dimascahyo Budianto Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Yunus, Muchammad Yusrizal Akmal Yustinasari, Lita Rakhma Yustisiane Ruth Rahadi Zahro, Vida Seanita Zhaza Afililla Zulkifli, Arief Hidayat