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Sri Subekti
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Isolasi dan Identifikasi Bakteri yang Terdapat pada Saluran Pencernaan Ikan Gurami (Osphronemus gouramy) dengan Pemberian Pakan Komersil yang Berbeda [Isolation And Identification Of Bacterial In The Digestive Organ Of Gurami Fish (Osphronemus gouramy) With Different Commercial Feed] Sri Subekti; Franch Dalahi; Agustono Agustono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11385

Abstract

Abstract Based on the food habits, gurami is omnivorous fish that feed herbivorous carp tend consists of natural food and artificial feed (pellets). The feed is needed to support the growth and survival of Gurami. If feed is given in accordance with the eating habits of Gurami and contain high nutritional Gurami can then growth accelerated faster. Some types of bacteria found in the digestive tract of animals has an important role in order to improve feed utilization, fish health, and environmental quality improvement and microorganisms. In addition, some bacterial flora in the gastrointestinal tract plays an important role and produce several types of enzymes in the digestive tract may contribute to the metabolism of the host. The purpose of this study was to determine the bacteria in the digestive tract of Gurami (Osphronemus gouramy) were given three different types of commercial feed. This study begins with a sampling of Gurami (Oshpronemus gouramy) of maintenance in the Faculty of Fisheries and Marine at Airlangga University in Surabaya and then do stage isolation and identification of bacteria in laboratory bacteria and Microbiology, Faculty of Veterinary Medicine, Airlangga University Surabaya. This study was conducted in July 2013. This research is a descriptive study, the method of solving the problem by describing the subject matter into a form that is easy to understand and based on facts. Results of research on gurami fish that given by three different types of commercial feed, there are two types of bacteria that dominate in the digestive tract of carp, the Pseudomonas sp. and Serratia sp. Both types of bacteria has the potential to help the digestive system of gurami fish. The result of this research are need for further research using the bacterium Pseudomonas sp. and Serratia sp. as probiotics.
Bioaktivitas Minyak Atsiri Daun Bandotan (Ageratum conyzoides) Terhadap Respon Fisiologis (Glukosa Darah Dan Tachiventilasi) Ikan Koi (Cyprinus carpio) [Bioactivity Essential Oil Of Bandotan Leaf (Ageratum conyzoides) On Koi (Cyprinus carpio) Physiological Responses (Blood Glucose And Tachiventilation) ] Laksmi Sulmartiwi; Mardiah Rahma Umami; Moch. Amin Alamsjah; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11388

Abstract

Abstract Koi (C. carpio) is one of famous ornamental fish in Indonesia. Fish often be subject of change or stessor like physical, chemical and biology on culture. Stress in fish caused released cortisol hormone and cathecolamine hormone. Cathecolamine increase glicogenolisis, cardiovascular regulation and respiration fungtion. Blood glucose and tachiventilation (opercular movement) can indicate stress respon in fish. Essential oil of A. conyzoides have central analgesic activity. Analgesic drugs in vertebrae can reduce stress in fish. Essential oil shown inhibitory effect of GABA transminase, an enzime GABA (gammaaminobutryic acid) degradation which can provoking sedation. Sedation effect in fish reduce blood glucose and tachiventilation. Potency bioactive essential oil of bandotan leaf compound haven't been know in aquaculture. The potency based of LC50 (Lethal Consentration 50 %) value Purpose of this research determined effect of essential oil Ageratum conyzoides leaf on Koi (Cyprinus carpio) Phsycology Response (Blood Glucose and Tachiventilation) every 4 hours durung 24 hours and LC50-24 hours. Research was conducted at Laboratory of Faculty of Fisheries and Marine Airlangga University Surabaya. Research design used Completely Randomized Design and followed by Duncan's Multiple range test. Results of analysis of variance (ANAVA) from early observation and every 4 hours during 24 hours day showed that each treatment of essential oil Ageratum conyzoides leaf gave a significantly different effect on the blood glucose and tachiventilation (P <0.05). The highest average of blood glucose was found in D treatment at second observation (443,67 mg/dl) and the lowest was found in C treatment (60,67 mg/dl). The highest average of tachiventilation was found in D treatment at first observation (1730 bit/9 minute) and the lowest was found in A treatment (437,67 bit/9 minute) at early observation. LC50-24 hours essenial oil Ageratum conyzoides leaf is 34,047 ppm. Based on measurements of water quality, treatments was still viable and good to supported viability of Cyprinus carpio during research, water temperature range between 29-31oC, range of dissolved oxigen 4-6 mg/l, pH ranges 7,5-8,3 and 0,0060,27 mg/l on ammonia.
Substitusi Tepung Bungkil Kedelai dengan Fermentasi Daun Lamtoro (Leucaena glauca) Terhadap Pertumbuhan dan Efisiensi Pakan Ikan Nila (Oreochromis niloticus) [Subtitution Of Soybean Meal With Fermented Lamtoro (Leucaena glauca) On Growth And Feed Efficiency Of Tilapia Fish (Oreochromis niloticus) ] Agustono Agustono; Novi Krishatno Putera; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i2.11410

Abstract

Abstract Tilapia fish (Oreochromis niloticus) was known as an important commodity in the world's freshwater. Tilapia fish (O. niloticus) was a omnivores species of fish, which means that can eat plants and animals. Tilapia fish easy to breed, fast growing, relatively has large body size, disease resistance, easy to adapt to the environment, the price was relatively cheap and has a high nutritional value as a source of animal protein. One of the obstacles faced in the feed manufacturing was an expensive raw material availability, therefore, necessary to find alternative feed ingredients that were cheap, quality and be available at all the times. The purpose of this study was to determine the effect of the use lamtoro were fermented with probiotic on growth and feed efficiency of tilapia fish. The research was conducted at the Faculty of Fisheries and Marine University. This study was conducted for 42 days from 25 March - 5 May 2013. The research design used was a completely randomized design (CRD). Materials used in this study was tilapia fish aged 1 month, the body length ranges between 3-5 cm and body weight ranged from 1,3-1,5 gram as 100 individuals. Form of feed pellets substituted with lamtoro fermented dose A. 0% (control), B. 10%, C. 20%, D. 30%, E. 40% with 4 replications. The main parameters observed in this research were the growth rate and feed efficiency. Support parameters was the quality of water. Based on the results of research about substitution of soybean meal with fermented lamtoro in the feed rations were no significantly different effect on the rate of growth and feed efficiency (p> 0.05) due to dissolved oxygen content does not qualify as well as, higher quality feed than feed quality substituted.
Korelasi Pertumbuhan dan Kandungan Karaginan Kappaphycus alvarezii Dan Eucheuma Spinosum Dengan Jarak Penempatan Rakit Apung Yang Berbeda Di Desa Grujugan Kecamatan Gapura Kabupaten Sumenep [Correlation Of Growth And Carragenan Content Of Kappaphycus alvarezii And Eucheuma Spinosum By Different Distance Placement Of Floating Rafts At The Grujugan Village, Sub-District Gapura, Residence Of Sumenep] Sri Subekti; Lina Wafia Asmi; Moch. Amin Alamsjah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i2.11412

Abstract

Abstract The selection of right location is an important succeed factor of the seaweed cultivation to make seaweed a good growth. Seaweed Kappaphycus alvarezii and Eucheuma spinosum contain carrageenan which has been widely used in several industries. The aim of the research is to determine the correlation of growth and carrageenan content K. alvarezii and E. spinosum by different distance placement of floating rafts. Research method using experimental method and experimental design using randomized block design, followed by Duncan's test or Mann Whitney U test and furthermore linear correlation to determine the correlation between growth and carrageenan content. The treatment using different cultivation locations. On K. alvarezii with a distance of 100 m from the beach (RK1), a distance of 400 m from the beach (RK2), and a distance of 700 m from the beach (RK3). E. spinosum at a distance of 100 m from the beach (RE1), a distance of 400 m from the beach (RE2), and the distance of 700 m from the beach (RE3). Each treatment was repeated 9 times. The main parameters observed in this study is the growth rate of once every 10 days, and the content of carrageenan obtained from the extraction of the final harvest. Supporting water quality parameters include: temperature, water transparency, current velocity, substance nutrients (nitrate and phosphate), salinity, and pH. The results showed seaweed K. alvarezii and E. spinosum cultivated at a distance at 400 m from beach has the highest growth rate and yield best carrageenan content. From the results of the calculation of growth and carrageenan content K. alvarezii and E. spinosum with distance floating rafts of different placements had a very strong correlation
Prevalensi Ektoparasit yang Menyerang Benih Ikan Koi (Cyprinus carpio) di Bursa Ikan Hias Surabaya [The Prevalence Of Ectoparasits That Attack The Seed Of Koi Fish In The Stock Of Ornamental Fish Surabaya] Sri Subekti; Novy Prasetya; Kismiyati Kismiyati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11434

Abstract

Abstract As the commodity grown, ornamental fish has an interesting added value. Many things can serve as a supporting factor in conducting the business of ornamental fish, such as having economic value and many enthusiasts who already spread to the whole society. One major problem in the cultivation of ornamental fish in Indonesia to this day is about the disease. One of the diseases that often strike in the ornamental fish pond is parasiter disease. In anticipation of losses due to parasitic attacks on fish, is necessary to identify types of ectoparasites that attack the seed of koi fish. This is done sejal early, so that the seeds are already carrying the disease agent could negatively impact on the enlargement and not widespread. The purpose of this study was to determine the type of ectoparasites that attack koi fish and find out the prevalence of ectoparasites that attack koi fish (Cyprinus carpio) in East Java at Prapen Surabaya The method used is a survey method for gathering informationfrom most of the population is considered to represent a particular population. Types of ectoparasites found were Trichodina sp., Chilodonella sp., Myxobollus sp., Ichtyopthirius multifilis, Dactylogyrus sp, Gyrodactylus sp., And Argulus japonicus. Prevalence rate of ectoparasites that infested fish Koi (Cyprinus carpio) in Surabaya fish stock is Trichodina sp. 84%, Chilodonella sp. 31%, Myxobollus sp. 17%, Ichtyopthirius multifilis 4%, 13% Dactylogyrus sp., Gyrodactylus sp. 7% and Argulus sp. 14%.
Kandungan Bahan Kering, Serat Kasar dan Protein Kasar pada Daun Lamtoro (Leucaena glauca) yang Difermentasi dengan Probiotik sebagai Bahan Pakan Ikan [The Content Of Dry Matter, Crude Fiber and Protein from Fermentation Of Leucaena glauca Leaf Using Probiotic As Fish Feed Material] Agustono Agustono; Devy Rahmawati Putri; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 2 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i2.11567

Abstract

Abstract Feed is the element that really support the activities of aquaculture, there for the feed that is available must be adequate and meet the needs of the fish. Efforts to reduce feed costs, some farmers use alternative feed ingredients as a substitute for feed ingredients. Lamtoro leaves in the form of a mixture of flour can be used as fish feed in pellet form. This research been used method with used Completely Randomized Design (CRD) with five treatments and four replications. The treatments used were control (P0), the provision of Probiotics 2% (P1), giving Probiotic 4% (P2), Probiotics administration 6% (P3) and the provision of Probiotics 8% (P4) with each repeated 4 times. Parameters observed were protein content of crude fiber and crude after fermentation for 7 days. These results indicate that administration of probiotics 8% gave significant effect toward crude fiber content in the leaves are fermented and lamtoro which been given Probiotics 6% gave a highly significant effect of the increase in crude protein content in the fermented leaves lamtoro.
Identifikasi dan Prevalensi Ektoparasit pada Ikan Kerapu Tikus (Cromileptes altivelis) di Karamba Jaring Apung Unit Pengelola Budidaya Laut Situbondo [ Identification And Prevalence Of Ectoparasites In Grouper (Cromileptes altivelis) At Floating Net Cage Of Marineculture Management Unit Situbondo] Sri Subekti; Rahayu Kusdarwati; Ferlyn Hendra Wiyatno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11592

Abstract

Abstract Grouper is a fish that lives in coral reefs, which is the internationally known as groupers or coral reef fishes. These fish have high economic value and potential to be developed in Indonesia. Grouper traded alive, the price is relatively high. The price of grouper at the level fishermen to reach US$ 20 (Rp 200,000, -) for each kilogram.These fish are exported mainly to Hong Kong with high price. Development of grouper aquaculture in floating net a viable alternative to overcome the increasing production of marine fisheries. The problem that often inhibiting fish culture is the diseases emergence, among others, caused by the parasites. The emergence of the disease is the result of interaction between environmental conditions that dont support the cultivation of their life inside, fish (host) that are sensitive and the presence of parasites. Uncontrolled environmental conditions there for the fish become stressed with poor immune systems, and facilitate pathogenic substance attacked hosts. This study aimed to the identify ectoparasites that attacked the grouper (Cromileptes altivelis) in floating net cages and to determine the prevalence rate of ectoparasites that attacked the grouper (Cromileptes altivelis) in floating net cages. The method of this study was descriptive method. Samples were taken with body length between 15-20 cm and four months old from the floating net. The main parameters observed in this study was identification of ectoparasites that attacked the grouper (Cromileptes altivelis) in floating net cages and prevalence rates for each of ectoparasites. While the supporting parameters were that water quality value in floating net cages as follow temperature, pH and salinity were measured during sampling activities. The results showed that of 60 samples were taken from four plots of floating net cages, 21 fish infected with ectoparasites positive. 17 positive fishes infected Pseudorhabdosynochus sp., one sample positive infected Benedenia sp. 3samples positive infected fish Neobenedenia sp. The ectoparasites prevalence of from cages 1, 2, 3 and 4 of 26.66%, 33.33% , 53.33% and 26.66% The suggestion of this study is cleanness improvement of floating net cage management to reduce ectoparasites infectation.
Uji Sensitifitas Sari Buah Pare (Momordica charantia L) pada Bakteri Edwardsiella tarda dengan Metode Difusi Kertas Cakram Secara In Vitro [Sensitivity Test Of Fruit Bitter Melon Juice (Momordica charantia L) On Bacteria Edwardsiella tarda With Paper Disc Diffusion Method In Vitro] Sudarno Sudarno; Selvi Lely Rosanti; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11593

Abstract

Abstract One of the problems encountered in fish farming is the disease attack. The attack of disease that was caused by bacteria is a problem that often occur in aquaculture. Traditional medicine is more readily accepted by communities because is cheap and easily available, have been quite familiar with the community. There were various kind of traditional medicine and has been widely studied chemically content and usefulness. Therefore there were still many plants unknown levels of toxicity, therefore need further study. Bacteria E. tarda spread in some countries such as Europe, Japan, Taiwan, Thailand, United States, Singapore, and Malaysia. While in Indonesia E. tarda were found in Java, Sumatra, and Borneo. The aim of the research is to know the capabilities of the inhibition zone of fruit bitter melon power against the bacteria E. tarda in vitro and knowing whether fruit bitter melon effective in inhibiting the growth of the bacteria E. tarda with gold standard comparator antibiotics Tetracycline. The research was conducted on February 2011 in Bacteriology and Microbiology, laboratory in Fish Quarantine Juanda Sidoarjo district of East Java. The method used in this research is descriptive method that was done in vitro by using sensitivity test of diffusion method with the determination of inhibition zone by comparing with antibiotic inhibition zones. The results showed that bitter melon juice has a bacterial growth inhibition against E. tarda, although when was compared with gold standard antibiotic tetracycline shared considered less sensitive. Observations showed clear zone of bitter melon fruit juice shared with a diameter of 13.3mm, was compared with standard antibiotic tetracycline can inhibit with a minimum diameter of 15mm. Sensitivity test of bitter melon fruit juice will be classified as not susceptible to the bacteria therefore is able to inhibit E. tarda..
Pengaruh Fermentasi Limbah Rumput Laut Gracilaria sp. dengan Bacillus subtilis Terhadap Populasi Plankton Chlorophyceae [Effect Of Waste Seaweed Fermentation Of Gracilaria sp. With Bacillus subtilis Against On Plankton Populations Of Chlorophyceae] Moch. Amin Alamsjah; Rani Frisca Christiana; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11607

Abstract

Abstract One of Indonesia's marine living resources is seaweed. There are about 18,000 species of seaweed around the world and 25 species of which have high economic value. In indonesia, there are 555 types of seaweed and four types of which are known as export commodities, that is Euchema sp., Gracilaria sp., Gelidium sp. and Sargasum sp. One type of algae is much cultivated in the waters of Indonesia is Gracilaria sp. Seaweed is widely cultivated because it has an important role in the effort to increase fish production and preservation of biological resources. However, sometimes the other result are found a lot of waste of seaweed in the field. Utilization of seaweed waste of Gracilaria sp. can be applied into organic fertilizer that is through a biological fermentation process using microbial proteolytic. The aims of the study to determine the effect of the waste seaweed fermentation of Gracilaria sp. with Bacillus subtilis on plankton populations of Chlorophyceae. The research was perform in August 2011 at the Laboratory of Fisheries Education, Faculty of Fisheries and Marine, University of Airlangga, Surabaya. The design of experiments in this study using Completely Randomized Design (CRD) with 6 treatments and 4 replications. The treatments were: (A) control, (B) 10ml/l of seaweed waste liquid without fermentation, (C) 5ml/l of seaweed waste liquid using fermentation, (D) 10ml/l of seaweed waste liquid using fermentation, (E) 15ml/l of seaweed waste liquid using fermentation, (F) 20ml/l of seaweed waste liquid using fermentation. The main parameter is plankton populations of Chlorophyceae, while supporting parameter water quality. Data analysis using analysis of variance (ANOVA), when there is a difference followed by Duncan's Multiple range test. The results showed that the utilization of waste seaweed of Gracilaria sp. Using fermentation with isolated bacterial of Bacillus subtilis produce significantly different population (p <0.05) and D the treatment that is 10ml/l of seaweed waste liquid using fermentation, showed the highest cell density of 264,252 cells / ml. Based on the results of Duncan's Multiple range test showed that the D treatment give the best effect among all of the treatments. Water quality parameters during the study remained within the tolerance limit for the growth of Chlorophyceae, pH 7-8 and the water temperature ranges between 2729o C.
Pengaruh Lama Penyinaran Terhadap Pertumbuhan dan Klorofil A Gracilaria verrucosa Pada Sistem Budidaya Indoor [The Influence Of Radiation Time Of Growth and Chlorophyll A Gracilaria verrucosa In Indoor Cultivation System] Moch. Amin Alamsjah; Nurines Oktavia Ayuningtiaz; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i1.11667

Abstract

AbstractGracilaria cultivation is basically in need of lighting for photosynthesis process which will be a great effect on growth. The use of fluorescent (FL) as a replacement for the sun light is used for fluorescent lamps produce white light, except that fluorescent lights do not increase the room temperature drastically culture (temperature stable) and the intensity of light produced in the culture room suitable for the growth of shoots Gracilaria verrucosa. In addition to light intensity, while irradiation also affects the growth of cultured algae. Long exposures are generally set according to the needs of algae in natural conditions. In the process of photosynthesis is need a light and dark reaction. Light reaction occurs in the conversion of light energy into chemical energy and dark reactions occur for the formation of glucose that used for catabolism system. This study aims to determine the best long exposures of the different long exposures which can affect the growth and the amount of chlorophyll a of G. verrucosa. The methods is an experimental that used Complete Random Design (RAL) as the experimental design. The treatment used is different irradiation time, the treatment A (long irradiation for 24 hours, control), treatment B (irradiation time 12 hours light: 12 hours dark), treatment C (irradiation time 8 hours light: 16 hours dark) and D treatment (irradiation time 16 hours light: 8 hours dark) of each treatment is repeated five times. The main parameters were observed daily growth rate (%/day) and the amount of chlorophyll a (µg/ml) of G. verrucosa. Supporting the observed parameters are pH, temperature (ºC) and salinity (%o) The analysis data is using of variants analysis (ANOVA), if there is a difference data followed by Duncan's Multiple Range Test to determine the best treatment. The results showed that different irradiation time take a real effect (P <0.05) on the growth and the amount of chlorophyll a G. verrucosa. Daily weight growth rate of the highest average found in treatment D (irradiation time 16hour light: 8-hours dark) of 0.62%/day, and the lowest in treatment A (24-hour long light irradiation) of 0.23%/day. The highest number of chlorophyll a average at the end of the study are in treatment D (irradiation time 16-hours light : 8 hours dark) of 0.0068 µg/ml and the lowest in treatment A (24-hour long light irradiation) of 0.0048 µg/ml. The increasing daily growth rate of G. verrucosa can used of the irradiation time 16-hours light : 8-hours dark.
Co-Authors A. Shofy Mubarak Abda Abda Achmad Amarudin Adam Sultoni Ade Fitri Noor Inaya Adelina Grace Aditya Gita Rohmatullah Agustien Zulaidah Agustina Agustina Agustine Zulaidah Agustono - Ahmad Syahrul Mubarak Aji, Joni Murti M Akhmad Taufiq Mukti Aksono HP., Eduardus Bimo Al Rasyid, Lalu Zainul Alfath, Muhammad Badar Aliffiansyah Rizky E Amadeus Michel Goein Ana, A Andriani, Devin Dwi Anggraeni, Rosmayniar Anggun Mayang Sari Anief Rufiyanto Annisa Nur R. P. Apipaturohmah, Pipa Apriyanti, Eny Arifin, Riyadhil Haqqy Arini Arini Arrosyid, Muchson Arya Witantama Aryati Aryati Aryo Fajar Sunartomo Asradinto, Faris Fathan Aulia, Aviva Baswendro, Baswendro Baswindro Berliana, Suci Wahyu Budi Utomo Candra Agustin, Niyar Candra Wahyu Sportyawan Chasanah , Ummi Choirunisa, Safa Fayza Olivia Christian Simarmata, Thesalonica Christine J K Ekawati Cica Yulia Cicilia Dyah Sulistyaningrum Indrawati Damayanti, Wilda Ayu Desy, Yova Yunizar Devy Rahmawati Putri Dewi, Sutan Cadena Kusuma Diah Ayu Puspitarini Dian A, Shallomita Diky Zakaria Diwangkara, Naufal Kresna Dr. Woro Hastuti Satyantini, Ir., M.Si Dwi Yuli Pujiastuti Dwirahmadi, Feby Dwitha Nirmala Dyah Purwaningrum, Shintawati Dzati Utomo Edy Sudrajat, Agus Sarwo Eka Pramyrtha Hestianah Eka Saputra Ellis Endang Nikmawati Elroy A.W, Mada Endang Dewi Masithah Eny Apriyati Fathmawati Fathmawati Fauziah, Diana Fauziah, Dini Fa’adilla A, Nashwa Feby Dwirahmadi Ferlyn Hendra Wiyatno Florentina Sustini Franch Dalahi Galih Candra Prakosa Yudha Giovani Agnelli Susanti Gunanti Mahasri Halimatus Sa'diyah Hari B. Notobroto Hari Basuki Notobroto Harsasi Setyawati Hebert Adrianto Heny Arwati Herliana Arsianingtyas Hervina Benazir Ardiyanti Hutama Putra, Windra Ida Ayu Made Mahayani Ida Ayu Puspitasari Idha Kusumawati Iip Saripah, Iip Ike Nur Firdhayani Ilham Harlan Amarullah Indah S Tantular Jamilah, Dewi Siti January Jefry Prasetya Jefry, January Joni Murti Mulyo Aji Jumiyanto Widodo Juniastuti Juniastuti katiah, katiah Khairul Bariyah, Khairul Khuril Zaqyyah Kismiyati , Kismiyati, Kismiyati Kris Cahyo Mulyatno, Kris Cahyo Kurnia, Kiki Adi Kusnoto Kusnoto Kusumawardani, Yustika Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Lenny Widjayanthi Liliana Tedjo Saputra Lina Wafia Asmi Lindarto, Wira Widjaya Lucia Tri Suwanti, Lucia Tri Ludira Sari Luh Putu Suciati Mafinanik, Aliza Febrianti Maosul, Asep Mardiah Rahma Umami Margono, Mohammad Wahyu Mariana, Arni Retno Marina hasan Markus Suryo Utomo Masanori Kameoka, Masanori Mirni Lamid Mochammad Amin Alamsjah Mohamad Akmal A.H. Mohammad Rondhi Mohd Fazrul Hisam Abd Aziz Mohd Ihwan Zakariah Mokhamad Syaom Barliana Muhammad Aiman Yudarana Yudarana Muhammad Amin Muhammad Arief Muhammad Herman Muktiarni, Muktiarni Mupita, Jonah Mustapit , Mustapit Mustapit Mustapit Mustofa, Chairil Hana Musyafa, Muhammad Zainal Ali Naisah, Naila Hijrotin Nastiti, Amayliana Ajeng Nathania Disa Ariesta Andriani Ni Komang Ayu Artiningsih Nia Lestari, Nia Nina Herlina Novi Krishatno Putera Novy Prasetya Nugraha, Rifki Destrizal Nunuk Dyah Retno Lastuti Nur Fitriani Nurfauziana, Tazkia Nurines Oktavia Ayuningtiaz Nurul Dwi Novikarumsari, Nurul Dwi Nurul Kumalasari Nuzuluddin, Taufiq Rizza Oktofianus Sila Paqih, Khaidir Imam Patmawati Patmawati Patmawati Patricia Sembiring Meliala, Susan Barbara Patricia, Susan Barbara Permatasari, Nanda Prabawa, Dewa Gede Benny Raharja Pradiptya Ayu Harsita Puji Basuki Purwaningrum, Shintawati Dyah Puspa Wardhani Puspaningrum , Diah Puspaningrum, Diah Puteri, Whimas Jessica Ayudia Putri, Chairunnisa Ramadhani Putri, Delah Samanta R. Wahid Nur Yusuf R.TD Wisnu Broto Rachmi Masnilah Ragu Harming Kristina Ragu Harming Kristina Rahadianti, Dian Rahayu Kusdarwati Rahmadany, Deya Nathasya Rahmasari, Naila Dwi Rani Frisca Christiana Refi, Refi Rengga Eko Pradipta Renyta Andini, W. Retno Mawarini Sukmariningsih RHJ. Wedyowibowo Ririh Yudhastuti Ririh Yudhastuti Ririn Agustiya Riswanda, Andri Rizhar Eman Karunia Akbar Robi M.A Yahya Rohsarifuddin, Fadiya Furuujihim Rokhani Rokhani Rr. Juni Triastuti Sahidu, Adriana Monica Sahidu, Andriyana Monica Sahrun Salsabila, Salsabila Sampurna, Insan Cita Santi Martini Sapto Andriyono Sari, Dini Yurindiana Sari, Putri Desi Wulan Sarwo Edy Sudrajat, Agus Sasmitaningtyas, Maulidya Tri Sasmito, Adi Selvi Lely Rosanti Sembiring Meliala, Susan Barbara Patricia Septi Septi Setyawati, Febri Shifa Fauziyah Shifangi, Shifangi Shintawati Shintawati Sila, Oktofianus Siti Churrotin, Siti Sitti R. Umniati Soegeng Soegijanto Soehartono Soehartono Soehartono Sofia Sofia, Sofia Sri Kiswati Sri Sumarsih Sri Susilogati Sumarti Subagyo Yotopranoto Sudarko Sudarko Sudarno, Sudarno Suhartiningsih Dwi Nurcahyanti Sukaryo Sukaryo Sukaryo Sukaryo Sukaryo Sulistyowati, Jeni SUPRIYONO . Susiyanti, Rosi Sutrisno, Sutrisno Tanzilia, May Fanny Tegar Putra Purnayudha Teguh Hari Sucipto, Teguh Hari Tjahjaningsih, Wahju Tjahjaningsih, Wahju Tomohiro Kotaki, Tomohiro Tri Budhi Murdiarti Tri Budhi Murdiati Tri Budhi Murdiati Tumangkar, Totok Tutik Juniastuti Ueda, Shuhai Ulia Fajriah Ummi Chasanah Ummi Chasanah, Ummi Valentina, Virliana Titus Vemas, Vemas W, Rico Kusuma Wahyu Wahyu Wanti Wanti Wanti Wanti Wardana, Elizia Flauren Wardhani, Puspa Wasmen Manalu Wedyowibowo, RHJ Widi Astuti Widya Kristiyanti Putri WIHARDJO, EDY Wijoyono, Alvin Putra Wiranda G Piliang Wiranda G Piliang Wulandari Suryaningtyas, Endang Wulanndari, Wahyu Yadi Ruyadi Yarin Dwi Monica Yoes Prijatna Dachlan Yuli Pujiastuti, Dwi Yulia Rahmawati, Yulia Yuniar, Fahdia Maulita Yuristianti Utami, Aisyah Yuwono Marta Dinata Zahira, Naswa Alfina Zahra, Amanatta Zamawi, Irvan