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Journal : PENDIPA Journal of Science Education

 1 
IDENTIFIKASI BAKTERI Edwardsiella ictaluri PADA IKAN PATIN (Pangasionodon hypophthalmus) Manalu, Yemima Clarica; Aprilia, Dina; Anggraini, Heny; Efendi, Syachrani; Day, Dafzel; Astuti, Indri
PendIPA Journal of Science Education Vol 9 No 1 (2025): February
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.9.1.204-213

Abstract

Ikan patin (Pangasionodon hypophthalmus) merupakan salah satu jenis ikan yang banyak dibudidayakan dan diproduksi secara massal di Indonesia yang memiliki peluang pengembangan dalam skala industri sebagai ikan konsumsi karena mempunyai potensi pengembangan ekonomi yang tinggi. Namun dalam pengembangannya tidak terlepas dari permasalahan budidaya, salah satunya adanya serangan penyakit Enteric Septicemia of Catfish (ESC) yang disebabkan Edwarsiella ictaluri. Metode penelitian yang digunakan adalah praktik langsung terhadap ikan P. hypophthalmus dan diamati morfologi bakteri secara makroskopis dan mikroskopis, serta biokimiawi sesuai dengan standar uji biokimia. Hasil penelitian menunjukkan bahwa bakteri yang menginfeksi P. hypothlasmus adalah E. ictaluri.
Pemeriksaan TiLV (Tilapia Lake Virus) Pada Ikan Nila (Oreochromis niloticus) Budidaya Fitria, Dina; Fathonah, Deffya; Afrianti, Rini; Turrohmah, Azizah; Mujaidiah; Destiana, Anggari Linda; Day, Dafzel; Astuti, Indri
PendIPA Journal of Science Education Vol 9 No 2 (2025): June
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.9.2.310-317

Abstract

Nile Tilapia (Oreochromis niloticus) is highly sought after by Indonesian consumers and is one of the leading commodities cultivated in freshwater. However, tilapia farming still frequently encounters challenges that lead to a decline in production, one of which is infection by pathogens such as Tilapia Lake Virus (TiLV). TiLV can infect fish in waters with temperatures above 25°C, with mass mortality peaking at 30°C, reaching a 50% death rate. Proper handling and control measures are essential to manage TiLV outbreaks effectively. TiLV detection in fish can be conducted using the Polymerase Chain Reaction (PCR) method. PCR-based virus detection is a diagnostic technique that identifies the presence of viral genetic material with high specificity and accuracy. This study aimed to detect TiLV infection in farmed Nile tilapia. The procedures included sample collection, necropsy, organ fixation, RNA extraction, PCR amplification, electrophoresis, and gel documentation analysis. Based on the PCR results, Nile tilapia samples tested positive for TiLV infection, as indicated by the presence of a 250 bp DNA band in the gel documentation system, aligning with the TiLV positive marker. Among the three fish showing suspected TiLV symptoms, two (66.6%) were confirmed infected.
Co-Authors . Irawati . Martaliana, . . Rahmawati . Ramiyati . Yusnida, . A.A. Ketut Agung Cahyawan W Abas Yusuf Abdi, Egy Abdul Khair Junaidi Abdul Latief Afandi Afandi Afandi Agstria, Wiwil Cristin Agustina, Puput Radha ahmad yani Akidah, Ihramsari Alamul Huda, Alamul aloysius mering Amalia, Alisha Amallia Putri Amin Fauzi, Amin Amsa, Helfany Ana Fergina Anak Agung Gede Sugianthara Andy Usman Anggari Linda Destiana Anggraini, Heny Anibah, Anibah Annisa Setyanti, Annisa Apriani, Nani Astriani , Susi Astuti, Ade Ari Dwi Aunurahman, Aunurahman Aunurrahman Aunurrahman Aunurrahman AYU LESTARI Azhari, Munir Aziz, Qithfirul Azzahra, Azzahra Azzahrah, Dessy Bessie, Juita L.D. Burhan, Kistian Burhan, Kristian Busri Endang Bustari, Syamsul Candra Maulana, Candra Darini Day, Dafzel Deafirmanda, Yossi Deana, Miranda Putri Dede Suratman Desinta, Desinta Desni Yuniarni Devi, Nurwulan Kusuma Dina Aprilia, Dina Dina Fitria, Dina Duryana, Duryana Dwi Susanto Efendi, Syachrani Efrina, Gusnita Eny Enawaty Erna Purba Evania Suryaningsih, Evania Fadilah . Fadillah Fadillah Faizal Faridah Faridah Fathonah, Deffya Fazry, Saiful Febriani, Lina Dwi Fortanata linda Dewi Gita Deviana, Gita Gusti, Dermawanti Setyo Halida . Hanik Latifah, Hanik Harries Madiistriyatno, Harries Haryani Haryani Hazar, Suariti Herman, Ria Herry Sujaini I Gusti Bagus Wiksuana Ida Riana, Ida Iin Zheniar Winayati, Iin Zheniar Iskandar, Abdul Muis Istiqomah, Novita Nissa Jati, prihatina Juharita, Juharita Julia Julia Jumiarti, Ratna Kasim, Abas Khotif, Muhammad Kriss Diana Kristiono, Natal Kurniawan, Danni Candra Kurniawati, Happy Resa Lestiyaningsih, Ayu Luhur Wicaksono Lukmanulhakim ., Lukmanulhakim Luthfiah, Isnaini M Asrori, M M. Asrori M. Asrori ., M. Asrori M. Thamrin Maemonah, Maemonah Maharani, Syifa Manalu, Yemima Clarica Manggala, Esa Mansyuruddin, Mohammad Marisa Dwi Sukmawati, Marisa Dwi Marmawi R . Martono Martono Maygista, Irena Melati, Eka Mersytiana Atu Mery, Elisa Anna Messakh, Anthon B. Meylinie, Nieta Moenardy, Khalid Kasim Mohammad Asrori Mudinillah, Adam Muhamad Ali Muhammad Asrori Mujaidiah Murni Pratiwi Putri, Murni Pratiwi Musrifah Musrifah, Musrifah Mustaan, Mustaan Naemah, Tien Narsi (F54010014) Naser, Haspul Nazriati Nazriati dkk. Nilasari, Nilasari Noritawati, Ida Novia Dewi , Yosa Nurakhim, Bambang Nurfitrianty, Sri Nurhajijah, Nurhajijah Oktavianita, Oktavianita Pangestu, Gustin Restu Pawadi, Pawadi Polinus, Polinus Prastica, Ayunda Rahmadani Dwi Pratiwi, Nabilla Fujias Pratiwi, Popy Prihatiningrum, Ika Purwanti . PURWANTI PURWANTI Purwantini, Marta Ninik Pustika, Dewi Rabiah, Sitti RafiUddin, Hafiz Rahayu, Retno Suci Rahayu, Umi Puji Rahmah (F54009038) Ramadhani, Eliza Eka Rian, Rian Rianto, Leonov Ridho, Muhammad Mukharom Rini Afrianti Riska Yulanda Riyadah, Riyadah Rokhani, Siti Rokhiyah, Isti Rusianto, Eddy Santoso, Budiawan Sari, Donata Saripudin, Didin Setiawan, Fadli Sherly Franchisca Simare Mare, Gresmid Sisilawati, Sisilawati Siti Nuraisyah Siti Nurwiyanti Slamet, Rokhmad Soemardana, Soemardana Sri Wahyuningsih Suci Ramadhani, Suci Sugiyono Sugiyono Sumarni, RR. Anik Susanti, Yanthi Sutarmanto . Suyanto Suyanto Syahwani Umar Thamrin . Tris Wardani, Tris Turrohmah, Azizah urai salam Verona, Roswita Wiji Astutik, Wiji Yasin Efendi Yeni Kurniati Yolanda, Shanti Yudiarti, Arini Yuline . Yuline, Yuline Yuliyani (F54209055) Zefriyenni Zefriyenni, Zefriyenni Zurniati, Vivi Zurniti, Vivi