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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Agromet Jurnal Ilmu dan Teknologi Kelautan Tropis Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Laa Maisyir Jurnal Ekonomi Islam Indonesian Journal of Biotechnology Widyariset PROSIDING SEMINAR KIMIA KOVALEN: Jurnal Riset Kimia Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Jurnal Manajemen Aset Infrastruktur dan Fasilitas Kelola: Journal of Islamic Education Management Indonesian Aquaculture Journal Jurnal Riset Akuakultur Journal of Educational Research and Evaluation BJRA (Bongaya Journal of Research in Accounting) Efektor JSMA (Jurnal Sains Manajemen dan Akuntansi) Widyariset GANDRUNG: Jurnal Pengabdian Kepada Masyarakat Siyasatuna Jurnal Ilmiyah Mahasiswa Siyasah Syariyyah Jurnal Aktiva : Riset Akutansi dan Keuangan Qadauna: Jurnal Ilmiah Mahasiswa Hukum Keluarga Islam Buletin Poltanesa Journal of Applied Civil Engineering and Infrastructure Technology (JACEIT) Multidiciplinary Output Research for Actual and International Issue (Morfai Journal) Jumper : Jurnal Mahasiswa Pendidikan Olahraga Jurnal Media Akuakultur Indonesia Shautuna: Jurnal Ilmiah Mahasiswa Perbandingan Mazhab Arus Jurnal Sosial dan Humaniora Jurnal Pengabdian Inovasi dan Teknologi Kepada Masyarakat SinarFe7 Private Law Journal of Applied Sciences in Accounting, Finance, and Tax Jurnal Ilmiah Pendidikan Dasar (JIPDAS) El-Hadhanah: Indonesian Journal Of Family Law And Islamic Law Economics and Business Journal Jurnal Kedokteran Hewan Jurnal Pengabdian Masyarakat Olahraga dan Kesehatan Vokatek : Jurnal Pengabdian Masyarakat PEDAMATH: Journal on Pedagogical Mathematics Information Technology Education Journal Al Fuadiy: Jurnal Hukum Keluarga Islam Economos : Jurnal Ekonomi dan Bisnis Journal Sultra Research of Law International Journal of Educational and Life Sciences (IJELS) Jurnal Media Akademik (JMA) ENLEKTURER: Journal of Islamic Studies Akrual: Jurnal Bisnis dan Akuntansi Kontemporer HISABUNA: Jurnal Ilmu Falak Journal of Fisheries & Marine Inovasi Teknologi Masyarakat Journal of Mathematics, Computation and Statistics (JMATHCOS) International Journal of Education, Vocational and Social Science Hikamatzu Journal of Multidisciplinary Research Jurnal Bioteknologi & Biosains Indonesia (JBBI) Bersama: Jurnal Pengabdian Kepada Masyarakat Issues in Mathematics Educations (IMED) Journal of Pedagogical and Teacher Professional Development Lumbung Ngabdi: Jurnal Pengabdian Masyarakat At-Tadris: Journal of Islamic Education J-Sil (Jurnal Teknik Sipil dan Lingkungan) Jurnal Bioteknologi & Biosains Indonesia Journal of Arts Education and Design
Alimuddin
Departemen Budidaya Perairan, Fakultas Perikanan Dan Kelautan, Institut Pertanian Bogor Jl. Agatis, Kampus IPB Dramaga 16680
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Journal : HAYATI Journal of Biosciences

 1 
Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine- . ALIMUDDIN; IRMA ANDRIYANI; MUHAMMAD ZAIRIN JUNIOR; HARTON ARFAH; ANNA OCTAVERA; ODANG CARMAN; GORO YOSHIZAKI
HAYATI Journal of Biosciences Vol. 18 No. 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.3.135

Abstract

Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.
Growth Response and Feed Utilization of Giant Gourami (Osphronemus goramy) Juvenile Feeding Different Protein Levels of the Diets Supplemented with Recombinant Growth Hormone DARMAWAN SETIA BUDI; . ALIMUDDIN; MUHAMMAD AGUS SUPRAYUDI
HAYATI Journal of Biosciences Vol. 22 No. 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1463.812 KB) | DOI: 10.4308/hjb.22.1.12

Abstract

The purpose of this study was to examine the effect of dietary supplementation with recombinant growth hormone (rGH) on the growth and dietary utility of juvenile giant gourami. The rGH was mixed with chicken egg yolk and sprayed on to artificial feed with different protein levels (34, 28, and 21%; isoenergy). Each treatment group of gourami was paired with a control group that received feed of the same protein level, but without rGH supplementation. Juvenile of giant gourami (weight 15.83 + 0.13 g) were fed diets containing rGH, to apparent satiation, 2 times a week. Fish were reared from less than 2 months old  for 42 days in 100 L glass aquaria at an initial density of 10 fish per aquarium. At the end of this period, the biomass and daily growth rate (SGR) of the fish were measured and the feed conversion ratio calculated and compared. Our data showed that fish fed rGH-supplemented diets experienced higher growth than fish in the control groups and showed that fish with higher protein diets experienced higher growth than the groups with less protein diets. The group with the highest biomass gain, SGR, and lowest feed conversion ratio (FCR) was the group fed a 34% protein diet supplemented with rGH. Furthermore, biomass gain, SGR, and FCR in the rGH treatment group with a 28% protein diet matched the measurements of the non-rGH control group receiving a 34% protein diet (P > 0.05). We conclude that giant juvenile gourami given feed supplemented with recombinant growth hormone will experience increased growth and dietary utility compared with gourami given the same feed without supplementation.
Growth, Morphology and Growth Related Hormone Level in Kappaphycus alvarezii Produced by Mass Selection in Gorontalo Waters, Indonesia Siti Fadilah; . Alimuddin; Petrus Rani Pong-Masak; Joko Santoso; Andi Parenrengi
HAYATI Journal of Biosciences Vol. 23 No. 1 (2016): January 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1072.105 KB) | DOI: 10.4308/hjb.23.1.29

Abstract

The use of high quality seed can support the success of the seaweed cultivation. This study was conducted to evaluate the growth performance, morphology and growth related hormone level of brown strain seaweed Kappaphycus alvarezii seed produced by mass selection. Selection was performed in the Tomini Gulf, Gorontalo, based on mass selection of seaweed seed protocol with a slight modification in cut-off 10% of the highest daily growth rate. Selection was carried out for four generations. The selected 4thgeneration of seed was then used in cultivation performance test in the Celebes Sea, North Gorontalo, for three production cycles. The results showed that the selected K. alvarezii has higher clump weight and daily growth rate, longer thallus, more number of branches, and shorter internodes compared to the unselected control and seaweed from the farmer as external control. Furthermore, total sugar content, levels of kinetin hormone and kinetin:indole-3-acetic acid ratio were higher in selected seaweeds than that of unselected control and external control. Thus, mass selection method could be used to produce high growth of seed, and kinetin and indole-3-acetic acid play an important role in growth of K. alvarezii.
Growth, Survival, and Body Composition of Transgenic Common Carp Cyprinus carpio 3rd Generation Expressing Tilapia Growth Hormone cDNA . Kurdianto; . Alimuddin; Nurly Faridah; Goro Yoshizaki; Sri Nuryati; Mia Setiawati
HAYATI Journal of Biosciences Vol. 23 No. 3 (2016): July 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.622 KB) | DOI: 10.4308/hjb.23.3.150

Abstract

Transgenic has been known as one of the applicable methods to improve growth performance of cultured fish. This study was performed to evaluate the growth performance, survival, and body composition of the 3rd generation of growth hormone (GH) transgenic common carp (TG). Juveniles (BW: 1.53 ± 0.03 g) were reared for 60 days in 250-L glass aquarium with stocking density of 25 fishes/aquarium. Fishes were fed with commercial feed (protein content 36%), three times a day to satiation. Growth and survival were measured every 20 days. Our results showed that TG fish has 1.49 times higher in average weight growth (p < 0.05) compared with the non-transgenic common carp (NT). Higher total feed consumption, survival, body protein content, protein and lipid retention, hepatosomatic index, and lower feed conversion ratio were also shown on TG fish compared with NT fish (p < 0.05). However, body lipid content and blood glucose level of TG fish were lower (p < 0.05) compared with the NT fish. Total ammonium nitrogen level in rearing media of TG fish was 51.78% lower (p < 0.05) than that of the NT fish. In conclusion, culturing of GH-TG common carp showed potential to achieve high productivity, efficient, and environmental-friendly aquaculture.
Behavior, Histopathology and Physiological Responses of Rat Fed Diets Containing Growth Hormone Transgenic Fish Meal Alimuddin Alimuddin; Sri Murtini; Nurly Faridah; Sri Nuryati
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.678 KB) | DOI: 10.4308/hjb.26.1.1

Abstract

  The animal model response against genetically modified product may provide food safety information. This study was performed to observe behavior, histopathology and physiological responses of Wistar rat fed on the diet containing growth hormone (GH) transgenic common carp (Cyprinus carpio) meal (Ccm). Thirty rats of three-month-old (BW: 115.67–139.50g) were divided into five treatments (six rats per treatment). The treatments were rats fed on the commercial diet without Ccm (control), a re-pelleted diet containing 15% (NT-15) and 45% non-transgenic Ccm (NT-45), a re-pelleted diet containing 15% (TG-15) and 45% GH transgenic Ccm (TG-45). Rats were kept for three weeks, fed twice a day according to treatment by 30 g/day/rat and water was provided ad-libitum. Rat behavior was observed every day during feeding. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyvuric transaminase (SGPT), urea, and creatinine were measured at initial and the end of the experiment. Histology observation was made for liver, kidney, and brain tissues. Our results showed that behavior, histopathology, SGOT, SGPT, urea and creatinine levels of rat fed with control, NT- and TG-Ccm diets were similar. All rats survived until the end of the experiment. Thus, within the dose level of this study, GH transgenic Ccm showed no toxicity or effect on behavior and physiology of Wistar rats.
Co-Authors . Irmawati . Kurdianto . Suharsono A. Octavera A. Santika A.Anggi Reskiamalia Abdul Halim Talli Abdul Malik Abyadul Fitriah Abyadul Fitriyah Adi Sucipto, Adi Adinda Rizky Tasrianty Afdal Madein Afif Abdurrahman Afpriyaningrum MD Agus Oman Sudrajat Agus Priyadi Ahmad Faisal Ahmad Muzaki Ahmad Ramzi Ghulam Syam Ahmad Zaki Alfiah Mutmainnah Ali Alyaminy, Humaidatul Aminah Zaim Aman Sentosa Panggabean Amar Maruf Amin, Muhmmadiyah Andi Aliah Hidayani Andi Haerul Andi Maulana Kamri Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi, K. Petta Lolo, Arjuni Andri Gemaini Angela Mariana Lusiastuti Anggriani, Emi Anhar Anita zafirah ANNA OCTAVERA Anny Hary Ayu Apriwanto, Indra Aras Syazili Ardo, Okilanda Arief Boediono Arief Eko Prasetiyo Arien Heryansyah Arrayansyah, Faisal Asdar Asep Permana Asep Sopian Asri Ainun Amaliah Atradinal Aulia Asman Aulia Saputra Awi Dassa Ayi Santika Azis Ali Baderiah Baderiah Baderiah Bahri, Nur Alim Benyamin Timotiwu, Paul Bintang, Ilham Bohari Yusuf Boyun Handoyo Budimawan Burhan, Dinah Diyanah Chaeroni, Ahmad Chaerunnisa Chayati, Nurul Chotban, Sippah DARMAWAN SETIA BUDI Darussalam Darwin, Khadijah Darwis Darwis Said, Darwis DEDI JUSADI Delfian Masrura Deny Sapto Chondro Utomo Desy Nurul Astuti Devi Wardan Sari Dian Hardiantho Dian Meutia Sari Diki Nurbaldatun Islam Dinar Tri Soelistyowati Djadir Dwi Hany Yanti Eko Pramono Emma Suryati Emma Suryati Enang Harris Enang Harris Endi Permata Eni Kusrini Eni Kusrini Erma Primanita Hayuningtyas F.H. Pasaribu Fachruddin Fachruddin Fajar Anggraeni Fatihah, Anariski Fatimah Akbal Fauzan Wahib Alsani Fauziah Zainuddin Firman Firman Patawari Firmansyah Fitriadi Fitriyah Husnul Khotimah Fitry Hasdanita Fredinan Yulianda Frischa Faradilla Arwinda Mongan G. Yoshizaki Gemi Triastutik Gloria Ika Satriani Goro Yoshizaki GORO YOSHIZAKI Gunawan Gunawan Gustikasari Amin H. Arfah Hadi Nasbey Hafidah, Riva Halimah Hamda Hamid Habbe Hamzah Hanipa Hardiantho D Harmayani, Ria Harton Arfah Hary Krettiawan Haryanti Haryanti Hasan Nasrullah Hassan, Muhammad Nasiru Herjayanto, Muh. Hisyam Ihsan Husain , Muh. Saleh Ibnu Izzah Idrus Sahar Muhammad Iis Diatin Ika Kartika Ikhlas Amal Alam, Ade Ikhwanul Arifan ILHAM Ilham Minggi Imron Imron Indah Mastuti Insanul Arif Intan Febrianti Irma Andriani IRMA ANDRIYANI Irmawati, Budimawan Irvan Faizal Irvan Faizal Irwan Irzal Effendi Ita Djuwita Jasmadi Jasmadi JATI, M. SASMITO Jean-Christophe Avarre Joko Santoso Jr., Muhammad Zairin Jubaedah Julie Ekasari Jumrah123 K. Chaitanya Deepti K. Sumantadinata Kabangnga, Felix Jansen Kadriani, Nur Annisa Kamaruddin Kartika, Ni Made Andry Kartini, Selvira Febriani Katarina Manik, Tumiar Ketut Mahardika Ketut Sugama Khaerullah khairuzzazi Khayati, Sri Komar Sumantadinata Komar Sumantadinata Kurniawati, Wd. Intan Leman, Swanggi LESTARI DEWI, AYU Lianasari, Ika Yekti Lidyasari Lisa Risdianti Putri Liza Luky Adrianto Luthfy An-nisa Majid M. Zairin Junior M.Y. Jamal Madein, Afdal Maharani, Adinda Mariani, Yuni Marwah, Andi Marwah Mashud MEDIATY Megawati, Novi Mia Setiawati Miftahul Jannah Mira Mawardi MMSI Irfan ,S. Kom Moch. Noer Alim Mochamad Syaifudin, Mochamad Moh Syaiful Arif Muh Alias L. Rajamuddin Muh Silmi Kaffa Yusuf Muh. Akmal, Andi Muh. Alias L. Rajamuddin Muhamad Lutfi MUHAMMAD AGUS SUPRAYUDI Muhammad Ammar Naufal Muhammad Awal Ramadhan Muhammad Febriansyah Muhammad Firdaus Muhammad Ikhsan Muhammad Jufri Muhammad Muttaqin Muhammad Nasri Katman Muhammad Sabir Muhammad Safir Muhammad Wawan Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammadiyah Amin Muhram, La Ode Mukdin, Khairani Mukni’ah Munawaroh, Zuhrotul MUNTI YUHANA Muslim Muslim Mustakim Mustika Sari, Mustika Musyfikah Ilyas Muzahar N.A. Maswan Nabila Ulfa Nadhirah Nagu Nagu, Nadhirah Naila Izati Zalius Nasrullah Nefi Andriana Fajri Ni Made Andry Kartika Ni Putu Yuria Mendra Nining Asniar Ridzal Noerlitasari Nugraha, Adrianto Fahrizal Nunak Nafiqoh Nur Bambang Priyo Utomo Nur, La Ode Muh.Adam Nur, Rezki Amaliah Nurdin Arsyad Nurfadilla Nurhafida Nurhalija Nurhayati Nurhidayat, Muhammad Nurlaelah Nurlaelah Syarifuddin Nurly Faridah Nurly Faridah Nurmawati Musri Nurul Fadhilah Nurwati Djam’an O. Carman O.Z. Arifin Odang Carman Oman Unju Subandi P. Ratna Kumar Permata Sari, Anggun Petrus Rani Pong-Masak Priatama, Rizki Purwaningsih, Yuli Pustika Ratnawati Putri, Angelina Atika R Rifai R.D. Soejoedono Rahmat Hidayat, T Rahmat Ramdani Rahmi, Kurnia Anggraini Ramadhani Nur Yasfi Ramaita Ratu Siti Aliah Ridwan RIDWAN AFFANDI Risfiyanty, Dwi Kartika Riswandi Rizky Ayu Fatimah Ridwan Rodliyah Rohayu H, Rina Rosidah Rulhendri Rulhendri Rusli RUSTIDJA S. Mariam Safira Qisthina Wardhana Saleh, Andi Mulia Salwa Nur Salsabila Samsu Adi Rahman Sani, NurHaslina Sanusi Saputra, Ade Maulana Saputra, Mardepi Sari , Nur Indah Sari Budi Moria Sembiring Sari, Reska Handayani Sarmila Sekar Sulistyaning Hadiwibowo Setiawati M Shabarullah Shabrina Natasha Effendi Shalsyabila , Zabina Shandi Irawan Sholeh Ridwan Siddiq, Mustagfirah Sindi Fransisci Siska Yudiarti Siti Aslamyah Siti Fadilah Siti Khodijah Siti Nurul Haliza Sitti Fakhriyyah Sitti Zuhaerah Thalhah Slamet Subyakto Soelistyowati DT Soerja Koesnarpadi Soko Nuswantoro Sri Khayati sri murtini . Sri Nuryati Sri Nuryati Srihartini, Yusi St. Fatmawati. L St. Hidayah Triana St. Mukarramah Sudirman Sukenda . Sukenda Sukenda Sukmawati Ali Sumardin Raupu Sundari Sutamrin Syafrianto, Syafrianto Syuhada Restu Danupratama T. Takeuchi Tahrim, Tasdin Talib, Dr. Ahmad Tasdin Tahrim Tedy Murtedjo Tri Setyo Guntoro Usman Mulbar Utut Widyastuti Utut Widyastuti Utut Widyastuti UTUT WIDYASTUTI Viona Amelia Candra Wa Ode NurRadhiah Ridjalu Wahidah Sanusi Wartono Hadie Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Y. Hadiroseyani Yulintine Yulintine Yuni Mariani Yuni Puji Hastuti Yuningsih, Andri Yusra, Andi Yusuf, Niken Yulian Zairin Jr M Zakki Zainun